CN104388236A

CN104388236A - Grape wine as well as preparation method and application thereof

Info

Publication number: CN104388236A
Application number: CN201410776047.7A
Authority: CN
Inventors: 汪程远; 王元忠
Original assignee: CHONGQING LMLYA BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
Current assignee: CHONGQING LMLYA BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
Priority date: 2014-12-15
Filing date: 2014-12-15
Publication date: 2015-03-04
Anticipated expiration: 2034-12-15
Also published as: CN104388236B

Abstract

The invention belongs to the field of brewing grape wine and discloses a grape wine with high resveratrol content as well as a preparation method and application of the grape wine. The grape wine is prepared from the following traditional Chinese materials, which are in homology of medicine and food, including medlar, pachyma cocos, peanut vines, sealwort, cinnamon, mulberry fruits and grapes. The grape wine has the health preservation efficacy of the grape wine and also has the health preservation drug efficacy of the traditional Chinese materials. According to the grape wine disclosed by the invention, the deficiency of the traditional health wine of poor taste is improved, and the strong and mellow taste of the grape wine is also kept. Proved by experiments, as the resveratrol content of the grape wine is obviously higher than that of the traditional grape wine, the grape wine is good in quality. The grape wine accords with the requirements of compatibility of the traditional Chinese materials, does not have toxic and side effects, achieves the purposes of balancing and tonifying yin and yang, is suitable for both males and females, is capable of gently tonifying kidney, aiding liver and the kidney, invigorating blood and essence and strengthening bones and muscles and has the obvious efficacies of tonifying yang, improving sexuality, resisting fatigue and ageing, strengthening immunity, softening blood vessels, whitening face, keeping facial appearance, delaying ageing and the like.

Description

A kind of grape wine and preparation method thereof and application

Technical field

The invention belongs to grape wine brew field, be specifically related to a kind of grape wine and preparation method thereof and application, especially relate to high grape wine of a kind of Resveratrol content and preparation method thereof and application.

Background technology

Resveratrol (Resveratrol, be called for short Res), also known as resvertrol, chemistry by name trans-3,4,5-trihydroxy--stilbene (3,4,5-trihydrolystilbence), it is for resisting the polyphenol substance of Infected with Pathogenic Fungi in plant materials, and exist with free state resveratrol or combined polidatin at nature, the content of polidatin is generally higher than the content of its aglycon resveratrol.Resveratrol and polidatin have cis and trans dividing, and often exist with stable trans forms in plant, its trans-isomer(ide) physiologically active is better than cis-isomeride, and under uviolizing, trans resveratrol can be converted into cis-isomeride.The molecular structure of resveratrol (glycosides) is as follows

Resveratrol was just found as far back as nineteen twenty-four, within 1940, obtain first from the root of Mao Yeli (Yeramtrumgrandiflorum), Nonomura in 1963 etc. propose the effective constituent that resveratrol is some Chinese herbal treatment inflammation, cardiovascular disorder, adjustment lipid metabolism etc.In recent years, increasing research both at home and abroad shows, trans-resveratrol has various biological effectiveness.Trans-resveratrol can be combined by ER in human body, regulates Blood Cholesterol level, effectively reduces the danger of people's cardiovascular diseases; Trans-resveratrol can also suppress low-density lipoprotein peroxidation and platelet aggregation, regulates lipopoteins metaboilic level, reduces human body lipoprotein, has the atherosis and coronary heart disease effect of good prevention of arterial.Trans-resveratrol also has strong anti-oxidation and anti-free radical function, the trans-resveratrol of 1.3g/mL can obviously suppress Rat Erythrocytes autoxidation haemolysis and by H ₂o ₂the oxidative hemolysis caused, and have obvious restraining effect to the generation of the inside and outside lipid peroxide of mouse core, liver, brain, kidney.American scientist in 1997 has delivered the sensation that paper that resveratrol has an antitumour activity causes scientific circles on " science " magazine.Trans-resveratrol has anti-mutation and antitumous effect, can suppress cyclo-oxygenase and catalatic activity, all has suppression and even reverse effect in initial, propagation, development 3 main phase of cancer cells; Trans-resveratrol can make mouse skin cancer cells can reduce 98% at most, can induce the differentiation of people's promyelocytic leukemia cell line HL-60, is called the another green cancer therapy drug newly after taxol.The same year, chemical structure and a kind of female hormone-stilboestrol of the discovery such as doctor Gehm resveratrol are closely similar, can be combined and it is activated by ER in body, play the intracellular signaling effect of female hormone, the osteoporosis relevant to female dimacteric syndrome can be improved as natural phant female hormone.Trans-resveratrol has obvious radiate protective action, and affects bone metabolism etc.In addition, trans-resveratrol and derivative thereof also play an important role in liver protecting, activation longevity gene, control nervous system disease etc.Therefore resveratrol has become one of the focus of world today's functional food, medicine and other fields research.

Within 1976, Langcake and Pryce finds to there is resveratrol in grape leave, its synthesis suffer uv irradiating, mechanical wounding and fungi infestation time sharply strengthen, can resist grey mold the infecting of the grape pathogenic bacteria such as downy mildew, be called as plant antibiotic, resveratrol starts the attention being subject to grape breeding scholar and phytopathologist thus.1989 the World Health Organization (WHO) carry out " world's cardiovascular disorder Controlling System " research draw " French antinomy ", French living habit contain and cause the high risk factor-hypercholesterolemia of coronary heart disease, smoking and shortage motion, but their sickness rate of coronary heart disease is more much lower than North America people, it is relevant that research finds that this and Frenchman like to drink red wine, because be rich in physiologically active ingredient resveratrol in red wine.Trans-resveratrol as can be seen here in grape wine has important health-care effect to human body.

Current sales volume vinous is growing, and product category is various, and the quality good and the bad mixes.The content of the trans-resveratrol in current domestic common dry red winew is at 0.3-2.67mg/L, and the ideal health care dosage of trans-resveratrol is every daily ingestion 2-10mg/ days, reach health-care effect to need to drink a large amount of dry red winews, but a large amount of grape wine of drinking can cause taking in too much alcohol, and does not meet grape wine noble quality, elegant culture of sampling wine.

There is the preparation of wine improving trans-resveratrol amount at present, mainly can be summarized as following a few class:

1, with the grape of high Resveratrol content for grape wine prepared by raw material.If application number is the Chinese patent of CN200610129321.7, this invention induces the synthesis of trans-resveratrol by the grape variety being rich in trans-resveratrol and uv-radiation choosing specific producing region, adopt the trans-resveratrol in specific technique stripping Pericarpium Vitis viniferae as much as possible, and farthest prevent the oxidation of trans-resveratrol, the fine and smooth alcohol of vinosity and, elegant fragrance is noble, in limpid Ruby red.Method and the common Wine-making technique of this invention do not conflict, and do not need specific equipment, technical arrangement plan is quite convenient, and social benefit is huge.The grape variety of high trans-resveratrol is uv-radiation induction, and its toxigenic capacity is high, and the proterties of high trans-resveratrol is not easy heredity inherits, once after the proterties of high trans-resveratrol disappears, and can not the grape wine of continuous production height trans-resveratrol.

2, in grape wine, trans-resveratrol is added.As application number method disclosed in the Chinese patent of CN200880122683.0 comprises: add the trans-resveratrol being obtained from grape in a part for bulk wine, form trans-resveratrol enriched material, with in bulk wine, add described trans-resveratrol enriched material, produce trans-resveratrol increase grape wine.The production cost of the method is low.As application number be CN03112486.0 Chinese patent specification sheets in disclose production of high content white veratrum alkaloids spirit embodiment and disclose one ton of high-content of resveratrol drinks production instance: increase 10mg by often liter of grape wine Resveratrol content and calculate, trans-resveratrol 10 grams need be prepared, grape wine 1000kg, get grape wine appropriate, low-temperature distillation obtains more than 70%v/v grape wine seminal fluid 50mL, 10g trans-resveratrol is added in above-mentioned grape wine seminal fluid, after fully dissolving, add in 1000mL grape wine, after full and uniform, then this wine is added in one ton of grape wine, be uniformly mixed, obtained high-content of resveratrol grape wine.Aforesaid method needs additionally to provide trans-resveratrol, and trans-resveratrol is if the words cost of natural extract is higher, and natural resource are limited, and the method for chemosynthesis provides trans-resveratrol can cause disadvantageous effect by environment, does not meet the theory of environmental protection.

3, polydatin is changed into trans-resveratrol.Application number is that the Chinese patent of CN 201210539571.3 has opened a kind of method of enrichment trans-resveratrol in liquor brewing process, described method comprises: by brewing wine, under aspergillus tubigensis and/or yeast effect, progressively fermentation generates alcohol and multiple by product, and the polydatin simultaneously added changes into trans-resveratrol.The method can not change the brewing process of wine, but the enzyme that polydatin changes into trans-resveratrol is glucuroide, so cannot ensure that polydatin changes into the transformation efficiency of trans-resveratrol.

4, the Resveratrol content in grape is improved.Application number is disclose a kind of method raising Resveratrol content in grape leaf, grape stem and grape fruit in the Chinese patent of CN 201010509046.8, uses colloidal aluminum inductor to induce grape material.Trans-resveratrol in grape fruit and grape production related by-products (blade, stem) can be induced to raise, efficient, stable, low residue, low toxicity.But the residual of aluminium salt can not be ignored the harm of human body, and the pollution of a large amount of aluminium salt pair environment is also quite serious.

Therefore, how to improve the content of trans-resveratrol in grape wine and become a new research direction.

Summary of the invention

In view of this, the present invention is directed to the defect of prior art, grape wine that a kind of Resveratrol content is high and preparation method thereof and purposes are provided.

For realizing object of the present invention, the present invention adopts following technical scheme:

A kind of grape wine, is made up of following raw material: matrimony vine 50 parts-100 parts, 40 parts-100 parts, Poria cocos, Caulis arachidis hypogaeae 50 parts-100 parts, sealwort 50 parts-70 parts, Chinese cassia tree 25 parts-50 parts, 45 parts-60 parts, mulberry fruit, grape 20000 parts-34000 parts.

Present invention also offers a kind of preparation of wine, the 20000 pretreated grapes of weight part-34000 weight part and 50 weight part-100 weight part matrimony vines, 40 weight part-100 weight part Poria cocos, 50 weight part-100 weight part Caulis arachidis hypogaeaes, 50 weight part-70 weight part sealworts, 25 weight part-50 weight part Chinese cassia trees, 45 weight part-60 weight part mulberry fruits are mixed into row zymamsis, after through secondary fermentation, ageing, after allotment;

Or first the pretreated grape of 20000 weight part-34000 weight part is carried out zymamsis, after through secondary fermentation, ageing, then with 50 weight part-100 weight part matrimony vines, 40 weight part-100 weight part Poria cocos, 50 weight part-100 weight part Caulis arachidis hypogaeaes, 50 weight part-70 weight part sealworts, 25 weight part-50 weight part Chinese cassia trees, 45 weight part-60 weight part mulberry fruit mixing preparations.

In some embodiments, grape described in described preparation method is Merlot, Cabernet Sauvignon.

In some embodiments, pre-treatment described in described preparation method is the synthesis of inducing trans-resveratrol in grape after adopting Whitfield's ointment to spray process with uviolizing.

In some embodiments, in described preparation method, described SA concentration is 60mg/L-100mg/L, described UV inducing amount 0.9KJ/m ²-1.28KJ/m ², 310 seconds-430 seconds.

In some embodiments, in described preparation method, described zymamsis is 18%-25% for adjusting Sucus Vitis viniferae sugar degree, acid content is 0.6%-0.8%, inoculate the yeast of 0.3wt%-0.5wt%, 0.3wt%-0.5wt% polygalacturonase and 0.3wt%-0.5wt% glucuroide to ferment, control leavening temperature between yeast phase below 28-30 DEG C, ferment to fermented liquid residual sugar and be down to less than 0.06%.

In some embodiments, in described preparation method, described yeast is saccharomyces bayanus.

In some embodiments, in described preparation method, described polygalacturonase is HE GRAND CRU polygalacturonase.

Present invention also offers the grape wine that described preparation method obtains.

Present invention also offers described grape wine and prepare the purposes in the medicine for the treatment of and suffering from a deficiency of the kidney.

Grape wine of the present invention is made up of the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, Caulis arachidis hypogaeae, sealwort, Chinese cassia tree, mulberry fruit and grape.Not only there is the health maintenance effect of grape wine itself, and make it have the health drug effect of Chinese medicine.Also improve the deficiency that the mouthfeel of Traditional health care wine is not good enough simultaneously, maintain the mouthfeel of strong sweet-smelling vinous.Experiment shows that grape wine Resveratrol content of the present invention is apparently higher than import and domestic grape wine, and quality is more excellent.Grape wine of the present invention meets the requirement of traditional Chinese medicine compatibility, have no side effect, reach equilibrium between yin and yang with the object of mending, be not only applicable to male population, also be applicable to female group simultaneously, can gentle kidney tonifying, liver and kidney tonifying, replenishing vital essence and blood, strengthening the bones and muscles, have obvious establishing-Yang, improve sexuality, antifatigue, anti-ageing, strengthen that immunity, vessel softening, beautifying face and moistering lotion, enhancing are immune, the effect such as delay senility, to suffering from a deficiency of the kidney, symptom plays significant curative effect, is suitable for that body is tired, soreness of waist and knee joint, chilly are afraid of cold, the crowd that suffers from a deficiency of the kidney of impotence erectile problem, insomnia and dreamful sleep.

Embodiment

Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.

Wherein, described matrimony vine refers to commodity " wolfberry fruit ", substantially refers to the dry mature fruit deriving from lycium barbarum.Matrimony vine property is flat, taste is sweet, returns liver, kidney channel, has effect that is nourishing liver and kidney, replenishing vital essence to improve eyesight, lose for consumptive disease essence, soreness of waist and knee joint, dizzy tinnitus, impotence and seminal emission, the disease such as interior heat is quenched one's thirst, the deficiency of blood is sallow, blurred vision is not clear.

Poria cocos is the dry sclerotia of On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf.Poria cocos taste is sweet, light, and property is put down, the thoughts of returning home, lung, spleen, kidney channel, has effect of promoting diuresis to eliminate damp pathogen, invigorating the spleen, the peaceful heart, dizzyly to throb with fear for oedema oliguria, phlegm and retained fluid, insufficiency of the spleen food less, the disease such as loose stool has loose bowels, confused and worried, palpitation with fear insomnia.

Sealwort (formal name used at school: Polygonatum sibiricum), has another name called: polygonatum sibiricum Redoute, yellow chicken dish, shaft of a writing brush dish, claw ginseng, Rhizome of Tendrilleaf Solomonseal, shank are joined.For the rhizome of Liliaceae Polygonatum sealwort, P. cyrtonema, hot river sealwort, P. kingianum, polygonatum cirrhifolium Royle etc.Sealwort nature and flavor are sweet, have tonifying spleen, the effect that moistening lung is promoted the production of body fluid.

Chinese cassia tree (Classification system: Cinnamomum cassia Presl), having another name called beautiful osmanthus, male osmanthus, cajaput, great Gui, peppery osmanthus, safety tree, cassia, is the dry bark of canella Chinese cassia tree.Complement sun, warming the spleen and stomach, removing accumulated cold, promoting blood circulation.Control decline of the fire from the gate of life, the cold weak pulse of limb, yang depletion is collapsed, and stomachache is had loose bowels, and cold hernia is run quickly globefish, waist knee crymodynia, through closing abdominal mass, and cloudy subcutaneous ulcer, streamer, and empty sun is more floating, heat in the upper and cold in the lower.

Mulberry fruit is the mature fruit of Moraceae deciduous tree mulberry tree, and what deficiency of the liver and kindey and deficiency of blood essence were lost has a dizzy spell; Soreness of waist tinnitus; Early whitening of beard and hair; Insomnia and dreamful sleep; Tianjin wound is thirsty; Quench one's thirst; The dry constipation of intestines.

Grape wine of the present invention is made up of the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, Caulis arachidis hypogaeae, sealwort, Chinese cassia tree, mulberry fruit and grape.Not only there is the health maintenance effect of grape wine itself, and make it have the health drug effect of Chinese medicine.Meanwhile, also improve the deficiency that the mouthfeel of Traditional health care wine is not good enough, maintain the mouthfeel of strong sweet-smelling vinous.Experiment shows that grape wine Resveratrol content of the present invention is apparently higher than import and domestic grape wine, and quality is more excellent.

Present invention also offers a kind of preparation of wine, the 20000 pretreated grapes of weight part-34000 weight part and 50 weight part-100 weight part matrimony vines, 40 weight part-100 weight part Poria cocos, 50 weight part-100 weight part Caulis arachidis hypogaeaes, 50 weight part-70 weight part sealworts, 25 weight part-50 weight part Chinese cassia trees, 45 weight part-60 weight part mulberry fruits are mixed into row zymamsis, after allocate after secondary fermentation, ageing;

Medicine fermentative processing together with fresh grape not only can retain the nutritive ingredient in raw material, can also obtain the benefit materials that many microbial metabolisms produce, very favourable to HUMAN HEALTH.

Experiment shows, the grape wine Resveratrol content described medicinal material fermentation together with fresh grape obtained is rear and Chinese medicine mixed preparing grape wine apparently higher than fresh grape fermentation.Therefore, in some preferred embodiments, described preparation of wine for the 20000 pretreated grapes of weight part-34000 weight part and 50 weight part-100 weight part matrimony vines, 40 weight part-100 weight part Poria cocos, 50 weight part-100 weight part Caulis arachidis hypogaeaes, 50 weight part-70 weight part sealworts, 25 weight part-50 weight part Chinese cassia trees, 45 weight part-60 weight part mulberry fruits are mixed into row zymamsis, after allocate after secondary fermentation, ageing.

Wherein, in some embodiments, the described medicinal material mixed with grape pulverizes and sieves in advance.

In some preferred embodiments, sieve described in as crossing 14 mesh sieves.

In preparation method of the present invention, described grape can select any grape variety well known by persons skilled in the art.In some embodiments, grape described in preparation method of the present invention is Merlot or Cabernet Sauvignon.

In some embodiments, in described preparation method, the amount of application of described SA is that per kilogram grape sprays 100mlSA solution,

In some embodiments, in described preparation method, described SA concentration is 60mg/L-100mg/L.

In some embodiments, in described preparation method, described UV inducing amount is 0.9KJ/m ²-1.28KJ/m ², 310 seconds-430 seconds.

In certain embodiments, in described preparation method, described grape is Merlot, and SA concentration is 100mg/L, UV inducing amount is 430 seconds, 1.28KJ/m ².

In certain embodiments, in described preparation method, described grape is Cabernet Sauvignon, and SA concentration is 60mg/L, UV inducing amount is 310 seconds, 0.9KJ/m ².

After uviolizing, grape deposits 72h in darkroom after treatment, and zymamsis is carried out in then impurity elimination after washing, the broken grape slurry that obtains.

Wherein, in some embodiments, described washing is first with ortho-water cleaning, then uses washed with de-ionized water three times.

In some embodiments, described impurity elimination is specially and adopts craft or destemming crusher to remove carpopodium.

In some preferred embodiments, described yeast fermentation power (CO ₂) > 950ml, moisture content < 5%.

In some preferred embodiments, described polygalacturonase enzyme activity 400,000.

In some preferred embodiments, described glucuroide enzyme activity 100,000.

Stir 2-4 the every day in wherein said zymamsis with in the medicinal powder making floating and skin slag press-in Sucus Vitis viniferae.

In some embodiments, the method of described adjustment Sucus Vitis viniferae sugar degree and acid content is add sulfurous acid solution 0.066%-0.117% in the grape slurry of fragmentation, sulfur dioxide concentration is made to reach 0.004%-0.006%, add sucrose 11.9wt%-23.4wt%, control at 7 °-18 ° to make the alcoholic strength after fermentation.

In some preferred embodiments, in described preparation method, described yeast is saccharomyces bayanus.

In some preferred embodiments, in described preparation method, described polygalacturonase is HE GRANDCRU polygalacturonase.

In preparation method of the present invention, after zymamsis, carry out secondary fermentation and ageing.

In some embodiments, described secondary fermentation is for control in room temperature by broth temperature, and filter wine liquid separation skin slag and obtain original wine, the sulfurous gas adding 0.3%-0.5% in original wine carries out secondary fermentation.

After secondary fermentation, wine liquid is filled liquor storage vessel sealing to preserve, temperature controls at 18-25 DEG C, and period those skilled in the art can carry out malic-lactic acid fermentation as required.

In some embodiments, described ageing is that control temperature carries out ageing at 10-15 DEG C, relative humidity 85%-90%, and carries out down bucket, bucketful and clarifying treatment.

Preparation method of the present invention, allocates obtained wine liquid after ageing.

In some embodiments, described in be allocated as and measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product and carry out blending process, storage 20-30 days.

Preparation method of the present invention, also comprises the steps such as essence filter, sterilizing packing after allotment.

In some embodiments, described essence filter carries out essence filter for wine liquid being crossed 0.45 μm of filter membrane.

In some embodiments, described sterilizing is packed as and passes into sterilizer 90 DEG C/min quick sterilization, bottling sealing, sterilization 20min in the water of 70 ~ 72 DEG C.

Zymamsis described in preparation method of the present invention and secondary fermentation can be carried out in any one fermentor tank well known by persons skilled in the art, as good Buddhist nun's virtue fermentor tank, vertical spray fermentor tank etc.

In some embodiments, described fermentor tank is good Buddhist nun's virtue fermentor tank.

Mouse test result display vinous of the present invention, the grape wine of security and not dosing wine fermentation maintains an equal level, it can significantly improve mouse sperm quantity, activity and survival rate, increase mouse testis and epididymis tissue weight, correct deficiency of yang Mouse Weight, plasma cortisol, suprarenal gland weight, improve mouse hypoxia tolerance and Burden-Swimming Ability of KM etc., simultaneously these results medicinal liquor of being better than existing health care liquor for strengthening Yang (i.e. kidney-replenishing healthy medicated wine) and utilizing existing establishing-Yang formula of Chinese materia medica preparation to make, show grape wine energy yin yang complementarity of the present invention, equilibrium between yin and yang in mediation body, can nourishing blood to tranquillize the mind, reinforcing the kindey to control nocturnal emission, the all diseases caused suffering from a deficiency of the kidney and sub-health state have obvious curative effects.

In addition, of the present invention have level conditions to take obvious curative effects through clinical Patients with Kidney Deficiency Type:

A, take through clinical 90 routine impotence patients, the patient of 30-45 day 97% feels to refresh oneself, and energetic, take 6 months continuously, the patient of 95% can carry out normal sexual life, stops drink and does not rebound;

B, to take through clinical 10 routine patients, take 3 months continuously, 8 routine patient's sperm quantities increase 30-55%; Take through clinical 25 routine cold sperm patients, the patient of 20-30 day 97% feels that cold sperm phenomenon is alleviated, and in 2-3 month, the patient of 96% occurs without cold sperm phenomenon;

C, take through clinical 12 routine rheumatism, similar rheumatism, arthritic, the disease sites pain connecting drink 30-45 days 96% patients obviously alleviates, and connects drink 4 months 94% patients and represents and drinking period disease sites without obvious pain;

D, the patient that frequently to get up in the night to urinate through clinical 23 examples take, and take 30-45 days 99% patients frequency of getting up in the night to urinate and obviously reduce, connect drink after 3 months 98% patient without symptom of getting up in the night to urinate, and not rebound after stopping drinking;

E, to take through clinical 9 routine dysmenorrhoea patients, connect drink 2-3 month 97% patient and represent that dysmenorrhoea obviously alleviates, connect drink after 3 months, 96% patient stops after drink also without obvious dysmenorrhoea sense;

F, take through clinical 12 routine patients, connect drink 3-5 month 95% patient and represent that the elasticity of skin and glossiness increase, drink continuously, patient's sensory effect of 94% is obvious:

Therefore present invention also offers grape wine of the present invention and prepare the purposes in the medicine for the treatment of and suffering from a deficiency of the kidney.

Those skilled in the art can determine according to the age of user, body weight, sex, natural health situation, nutritional status etc. the instructions of taking that grape wine of the present invention is concrete.In some embodiments, grape wine instructions of taking of the present invention is 50-100ml every day, takes the best when having a meal, and connect drink once week visible obvious curative effects, long-term taking can be promoted longevity, and has no side effect.

Grape wine of the present invention is made up of the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, Caulis arachidis hypogaeae, sealwort, Chinese cassia tree, mulberry fruit and grape.Not only there is the health maintenance effect of grape wine itself, and make it have the health drug effect of Chinese medicine.Also improve the deficiency that the mouthfeel of Traditional health care wine is not good enough simultaneously, maintain the mouthfeel of strong sweet-smelling vinous.Experiment shows that grape wine Resveratrol content of the present invention is apparently higher than import and domestic grape wine, and quality is more excellent.Grape wine of the present invention meets the requirement of traditional Chinese medicine compatibility, have no side effect, reach equilibrium between yin and yang with the object of mending, be not only applicable to male population, also be applicable to female group simultaneously, can gentle kidney tonifying, liver and kidney tonifying, replenishing vital essence and blood, strengthening the bones and muscles, have obvious establishing-Yang, improve sexuality, antifatigue, anti-ageing, strengthen that immunity, vessel softening, beautifying face and moistering lotion, enhancing are immune, the effect such as delay senility, to suffering from a deficiency of the kidney, symptom plays significant curative effect, is suitable for that body is tired, soreness of waist and knee joint, chilly are afraid of cold, the crowd that suffers from a deficiency of the kidney of impotence erectile problem, insomnia and dreamful sleep.In addition alleviate by having various diseases such as prolapse of lumbar intervertebral disc, blockage of main and collateral channels, rheumatism chill, the empty chilly of body, menoxenia, qi and blood are not smooth the adjustment of kidney and improve.Grape wine formula provided by the invention does not add any non-traditional Chinese medicine ingredients, simultaneously these Chinese medicine excellent in taste, and self is with the taste of sweetness, and does not affect the mouthfeel of grape wine itself, and the effective constituent effect of medicine itself and the effect of grape wine itself.

In order to understand the present invention further, below in conjunction with embodiment, grape wine provided by the invention and preparation method thereof is described in detail.Wherein HE GRAND CRU polygalacturonase is purchased from French laffort company; Saccharomyces bayanus is purchased from Dou Yi bio tech ltd, Shanghai.

Embodiment 1: preparation method of the present invention prepares grape wine

1, take matrimony vine, Poria cocos, Caulis arachidis hypogaeae, sealwort, Chinese cassia tree, each 50 grams of mulberry fruit Chinese medicinal materials respectively, pulverized 14 object sieves, and loaded in good Buddhist nun's virtue fermentor tank.

2, get 20Kg fresh grape (Merlot, Cabernet Sauvignon etc.) in addition, first adopt Whitfield's ointment (SA) to spray the synthesis of trans-resveratrol in process rear ultraviolet (UV) radiation-induced grape.Concrete grammar is:

1), 20Kg fresh grape adopt Whitfield's ointment (SA) spray process, per kilogram grape sprays 100mlSA solution, and the suitable concentration Merlot that SA sprays is 100mg/L, Cabernet Sauvignon is deposit 72h in darkroom after 60mg/L process;

2), take out SA and spray the grape after process, with the synthesis from veratryl alcohol in ultraviolet (UV) radiation-induced grape, UV induces optimal dose Merlot to be 430 seconds (1.28KJ/m ²), Cabernet Sauvignon is 310 seconds (0.9KJ/m ²), deposit 72h in darkroom after process;

3), take out the grape that UV irradiates, first with ortho-water cleaning, then use washed with de-ionized water three times, adopt craft or destemming crusher to remove carpopodium, the fragmentation of fruit grain is obtained grape slurry, add in good Buddhist nun's virtue fermentor tank.

3, in the grape slurry of fragmentation, add sulfurous acid 0.066%, make sulfur dioxide concentration reach 0.004%, add 3.6kg sucrose, stir, adjustment Sucus Vitis viniferae sugar degree is 18%, and acid content is 0.6%, the saccharomyces bayanus (fermenting power (CO of inoculation 0.3wt% ₂) > 950ml moisture content < 5%) and 0.3wt%HE GRANDCRU polygalacturonase (enzyme activity 400,000) and 0.3wt% glucuroide (enzyme activity 100,000) ferment, leavening temperature is controlled below 28-30 DEG C between yeast phase, fermentation is down to less than 0.06% to fermented liquid residual sugar and is fermented, leavening temperature is controlled below 28-30 DEG C between yeast phase, every day stirs 2-4 time, makes the medicinal powder of floating and skin slag be pressed in Sucus Vitis viniferae;

4, the 9 angel's fermented liquid residual sugars that ferment are down to less than 0.06%, and broth temperature controls in room temperature, filter wine liquid separation skin slag and obtain original wine; The sulfurous gas adding 0.3% in original wine carries out secondary fermentation;

5, liquor storage vessel sealing preservation filled by wine, and temperature controls at 18-25 DEG C, and period carries out malic-lactic acid fermentation as required;

6, then control temperature carries out ageing at 10-15 DEG C, relative humidity 85%-90%, and carries out down bucket, bucketful and clarifying treatment;

7, measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product and carry out blending process, store 28 days; Wine liquid is crossed 0.45 μm of filter membrane and carry out essence filter, pass into sterilizer 90 DEG C/min quick sterilization, bottling sealing, in the water of 70-72 DEG C, sterilization 20min, is up to the standards and gets final product code, vanning, warehouse-in.

Embodiment 2: Resveratrol content measures

Sample: trans-resveratrol standard substance (purity is greater than 99%), purchased from Sigma-Aldrich company.

Reagent: methyl alcohol, acetonitrile are chromatographically pure, all the other reagent are analytical pure.

Plant and instrument: Waters e2695 liquid chromatograph (being furnished with Waters2998 diode-array detector and Empower data handling system, Waters, US); Chromatographic condition: chromatographic column is UltimateC ₁₈post (4.6*250mm, 5 μm). mobile phase A is acetonitrile, and Mobile phase B is 0.01% phosphate aqueous solution, isocratic elution, A:B=25:75 (v/v).Flow velocity 1.0m L/min, sample size is 20 μ L, and determined wavelength is 306nm, and column temperature is 35 DEG C.

Sample preparation: Example 1 obtains wine samples and commercial glucose wine sample respectively, centrifugal concentrating removing ethanol, add 10mL deionized water, with 10mL extraction into ethyl acetate enzymolysis solution, extract 4 times, collect extraction liquid centrifugal concentrating removing ethyl acetate, as test liquid after dissolving by 250 μ L moving phases (acetonitrile: 0.01% phosphate aqueous solution=25:75 (v/v)).

The preparation of standard solution: take 25mg trans-resveratrol standard substance, is settled to 25m L with dissolve with methanol, makes 1L storing solution.Getting appropriate storing solution methanol dilution becomes concentration to be the trans-resveratrol standard solution of 0.2mg/L, 0.5mg/L, 1.0mg/L, 1.5mg/L, 2.0mg/L, 5.0mg/L, 10mg/L.

Be placed on 365nm ultraviolet lamp place and irradiate 2h, make trans-resveratrol be partially converted into cis-resveratrol, and calculate cis-resveratrol and trans-resveratrol content by differential technique.

The content of table 1 resveratrol in wines

Evaluate quality vinous usually using Resveratrol content as important reference index, as can be seen from Table 1, commercially available import grape wine is higher than domestic grape wine Resveratrol content, quality is good, domestic grape wine, due to the deficiency in manufacture craft and store method, causes trans-resveratrol to lose more serious.And grape wine Resveratrol content provided by the invention is apparently higher than import and domestic grape wine, quality is more excellent.

Embodiment 3:

Get 20Kg fresh grape by method described in embodiment 1, first adopt Whitfield's ointment (SA) to spray the synthesis fermentation of trans-resveratrol in process rear ultraviolet (UV) radiation-induced grape for grape wine; Then matrimony vine, Poria cocos, Caulis arachidis hypogaeae, sealwort, Chinese cassia tree, each 50 grams of mulberry fruit Chinese medicinal materials is taken, pulverized 14 object sieves, the grape wine standby with fermentation mixes, measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product to carry out blending process, store 28 days; Wine liquid is crossed 0.45 μm of filter membrane and carry out essence filter, pass into sterilizer 90 DEG C/min quick sterilization, bottling sealing, in the water of 70-72 DEG C, sterilization 20min, is up to the standards and gets final product code, vanning, warehouse-in.

Detect Resveratrol content according to the detection method described in embodiment 2, compare with the grape wine prepared according to method described in embodiment 1 simultaneously, the results are shown in Table 2.

The different preparation method's Resveratrol content of table 2 compares

Processing scheme	Trans-resveratrol mg/L	Cis-resveratrol mg/L
			1	1.91	0.25
2	1.71	0.18

Note: processing scheme 1 is for according to method medicine fermentative processing together with fresh grape described in embodiment 1; Processing scheme 2 be fresh grape fermentation for after grape wine with Chinese medicine mixed preparing grape wine.

From table 2 result, the grape wine Resveratrol content that medicine fermentation together with fresh grape obtains is rear and Chinese medicine mixed preparing grape wine apparently higher than fresh grape fermentation.

Embodiment 4: different yeast is on the impact of fermentation for Resveratrol content vinous

By method described in embodiment 1, select different yeast to ferment respectively, other operations are identical with embodiment 1 with material mixture ratio.Detect the rear Resveratrol content of fermentation according to the detection method described in embodiment 2, the results are shown in Table 3.

The different yeast of table 3 is on the impact of fermentation for Resveratrol content vinous

Group	Trans-resveratrol mg/L	Cis-resveratrol mg/L
			Saccharomyces bayanus	2.04	0.48
RC212 yeast	1.82	0.34
			F15 yeast	1.63	0.18

From table 3 result, according to method described in embodiment 1, the grape wine Resveratrol content selecting saccharomyces bayanus fermentation standby is apparently higher than RC212 yeast fermentation and F15 yeast.

Embodiment 5: different polygalacturonase is on the impact of fermentation for Resveratrol content vinous

By method described in embodiment 1, select different polygalacturonases to ferment respectively, other operations are identical with embodiment 1 with material mixture ratio.Detect the rear Resveratrol content of fermentation according to the detection method described in embodiment 2, the results are shown in Table 4.

The different polygalacturonase of table 4 is on the impact of fermentation for Resveratrol content vinous

Group	Trans-resveratrol mg/L	Cis-resveratrol mg/L
			HE GRAND CRU polygalacturonase	2.04	0.48
HE polygalacturonase	1.87	0.37

From table 4 result, according to method described in embodiment 1, the grape wine Resveratrol content selecting HE GRAND CRU polygalacturonase fermentation standby is apparently higher than HE polygalacturonase.

Embodiment 6: safety evaluation is tested

Get grape wine and the same batch of each 1000ml of grape wine not adding medicinal material and ferment of the embodiment of the present invention 1 preparation, be evaporated to 50ml respectively, as grape wine test liquid.Separately get cleaning grade Kunming mouse 60, body weight 20 ± 2 grams, male and female half and half, every day early, evening respectively gives a grape wine and grape wine concentrated solution, at every turn for 0.2ml/10g mouse is heavy, give 7 days continuously, observe mouse outward appearance, behavior, appetite, body weight and stool and urine, result shows two clock test liquids and does not all occur unusual phenomenon, 60 none death of mouse, therefore cannot ask and calculate its medium lethal dose (LD50), only roughly can calculate that the maximum tolerance of its oral administration is the concentration >40ml/kg body weight of 1:20, it is quantity more than 1000 times, illustrate that grape wine edible safety of the present invention is without side effect effect.

Embodiment 7: mouse sperm impact test

Test grouping: commercially available three animal penes wine group (lot identification mark 20100725), commercially available deer antler wine group (lot identification mark 20100618), commercial glucose wine group (lot identification mark 20100813), embodiment 1 group, commercially available strength wine group (lot identification mark 20100821) and physiological saline group.

Test method: get cleaning grade male mice in kunming 60, body weight 20 ± 2 grams, be divided into 6 groups at random, give above-mentioned medicinal liquor, grape wine and physiological saline respectively, the heavy gavage of 0.1ml/10g mouse, once a day, continuous 7 days, animal was taken off cervical vertebra in the 8th day and put to death, win rapidly epididymis tissue, after scales/electronic balance weighing, be placed in mortar and grind, dilute 15 times with ringer solution, count sperm quantity under the microscope, and observe its activity situation, survival rate, sperm count conversion is ten thousand/10mg epididymis tissue, the results are shown in Table 5.

Table 5 sperm affect test-results ( n=10)

Group	Sperm quantity (ten thousand/10mg epididymis tissue)	Living spermatozoa percentage
			Physiological saline group	233.1±11.5	58.7±6.7％
Grape wine group	235.3±10.1	64.1±4.1％
			Embodiment 1 group	275.9±20.1	88.6±4.5％
Strength wine group	237.6±12.4	60.3±6.7％
			Three animal penes wine group	236.9±16.3	64.7±5.5％
Deer antler wine group	247.3±13.6	68.6±7.3％

The most directly reacting for male sex's insufficiency of kidney-YANG is exactly the decline of sexuality, and sperm quantity and survival rate are the important indicators of sexuality, and the raising of these indexs indicates the lifting of sexuality, shows to have improvement result to insufficiency of kidney-YANG.From table 5 result, gavage gives grape wine of the present invention, and mouse sperm quantity, survival rate are all higher, and is obviously better than existing commercially available medicinal liquor and wine product group and physiological saline group, show that grape wine of the present invention can improve sexuality, have significant curative effect to insufficiency of kidney-YANG symptom.

Embodiment 8: mouse sexual gland impact test

Test grouping: with embodiment 4 clustering method.

Test method: get cleaning grade male mice in kunming 60, body weight 20 ± 2 grams, be divided into 6 groups at random, give above-mentioned medicinal liquor, grape wine and physiological saline respectively, the heavy gavage of 0.1m1/10g mouse, once a day, continuous 7 days, animal was taken off cervical vertebra in 8th day to put to death, get each mouse preputial gland, prostate gland seminal vesicle, the fat that carefully removing tissue is accompanying, liquid on surface is sucked with filter paper, ten thousand/ balance on weigh, and conversion is on average every mouse testis and epididymis tissue wet, the results are shown in Table 6.

Table 6 sexual gland affect test-results ( n=10)

Group	Testis weight in wet base (mg)	Epididymis tissue wet (mg)
			Physiological saline group	45.7±3.3	83.6±10.3

Grape wine group	46.1±7.5	87.1±11.5
			Embodiment 1 group	57.5±12.4	113.2±12.4
Strength wine group	47.6±4.7	97.8±11.3
			Three animal penes wine group	50.1±7.3	97.4±12.7
Deer antler wine group	48.5±6.2	97.8±12.3

Impel the growth of sexual organ to be means of enhancing ability, and sexuality to improve be the reaction improving male sex's insufficiency of kidney-YANG.From table 6 result, grape wine of the present invention can increase testis and epididymis tissue wet, and is obviously better than existing commercially available medicinal liquor and wine product group and physiological saline group.Show that grape wine of the present invention has androgen-like action, the growth of sexual organ can be impelled, have significant curative effect to insufficiency of kidney-YANG symptom.

Embodiment 9: on the impact of mice with kidney-yang deficiency body weight, plasma cortisol, suprarenal gland seminal vesicle weights

Test grouping: with embodiment 4 clustering method.

Test method: cleaning grade male mice in kunming 120, body weight about 20 grams, subcutaneous injection 0.5% hydrocortisone 0.2m1//day, continuous 7 days.Visual animal body weight obviously alleviates around here, average out to about 21 grams, have an appointment 30% animal dead, all visible hair of surviving animals loosens, chilly, the insufficiency of kidney-YANG symptoms such as limbs are sent out cool, poor appetite, dispirited.Test the 8th day, animal is divided into 6 groups by body weight Stochastic Equilibrium, give above-mentioned 4 groups of medicinal liquor, grape wine and physiological saline respectively, the heavy gavage of 0.1ml/10g mouse, once a day, continuous 12 days, duration of test, every 4 days record body weight once.Test the 21st day, extract eyeball of mouse, get blood by orbital vein, anticoagulant heparin, after centrifugal, quantitatively draw blood plasma, by northern immunoreagent institute method, utilize EJ-2008r immunity counter, carry out plasma cortisol mensuration.Group difference is compared by the T method of inspection.Get blood to weigh simultaneously, and win bilateral adrenal glands seminal vesicle, put in Bownis liquid and fix 24 hours, then surface liquid is sucked with filter paper, carefully except its hetero-organizations such as degreases, ten thousand/balance weighs weight in wet base, and conversion is average every mouse suprarenal gland milligram weight in wet base, carry out statistical treatment by the method for inspection, the results are shown in Table 7.

Table 7 detected result

As shown in Table 7, grape wine of the present invention has obvious curative effects to mice with kidney-yang deficiency, can improve the situation that body weight reduces, plasma cortisol reduces, suprarenal gland weight reduces because subcutaneous injection hydrocortisone occurs, and result is better than physiological saline group and existing commercially available medicinal liquor and wine product group.Show that grape wine of the present invention not only has correction " deficiency of yang " Mouse Weight and the low effect of suprarenal gland endocrine function, but also there is the effect of promotion " deficiency of yang " animal adrenal gland weightening finish.

Embodiment 10: mouse mating simultaneous test

Test grouping: with embodiment 4 clustering method.

Test method: get healthy male mice in kunming 60 (body weight 20 ± 2 grams) and mate its acquired experience of 2 angels with female elementization of the same race (only mating front 48h abdominal injection progynon B 20 μ g/) female mice (body weight 20 ± 2 grams).Take out female mouse, male mouse is had three days off.Male mouse is divided into 6 groups, and each group gives above-mentioned 4 groups of medicinal liquor, grape wine and physiological saline respectively, and the heavy gavage of 0.1ml/10g mouse, every day 1 time, continuous 7 days, the 8th day male mice and female mouse mated by 1:5 quantity.Record its mating latent period (to the time of mouse the 1st mating from mating) and 1 hour mating number of times, the results are shown in Table 8.

Table 8 mouse mating test-results ( n=10)

Group	Average mating latent period (min)	Average mating number of times/hour
			Physiological saline group	29.8±2.9	4.3±0.1
Grape wine group	26.6±2.8	4.6±0.1
			Embodiment 1 group	16.6±2.3	9.8±0.3
Strength wine group	26.5±2.7	6.1±0.2
			Three animal penes wine group	25.8±2.9	5.5±0.1
Deer antler wine group	26.2±3.3	5.3±0.4

The raising of sexual potency improves the most directly embodying of male sex's insufficiency of kidney-YANG.As seen from the results in Table 8, grape wine of the present invention, compared with all the other 5 groups, shortens mating latent period, adds mating number of times.Show that grape wine of the present invention can improve sexual potency, have significant curative effect to insufficiency of kidney-YANG symptom.

Embodiment 11: mouse hypoxia tolerance simultaneous test

Test grouping: with embodiment 4 clustering method.

Test method: get healthy male mice in kunming (body weight 20 ± 2 grams) 60, be divided into 6 groups at random, often organize 10, each group gives above-mentioned 4 groups of medicinal liquor and physiological saline respectively, the heavy gavage of 0.1mL/10g mouse, every day 1 time, continuous 7 days, after 1 hour, each group of mouse is put into respectively in the 8th day gavage the 250mL wide-necked bottle (putting 1 mouse for every bottle) filling 5g sodica calx.With the airtight bottleneck of Vaseline, timing immediately, until breath stopped.For index, respectively organize hypoxia endurance time with mouse survival time in airtight wide-necked bottle, the results are shown in Table 9.

Table 9 mouse hypoxia endurance test result ( n=10)

Group	Hypoxia endurance time (min)
		Physiological saline group	15.9±1.5
Grape wine group	21.1±1.3
		Embodiment 1 group	23.4±1.2
Strength wine group	18.1±1.1
		Three animal penes wine group	17.3±1.7
Deer antler wine group	18.3±1.1

Insufficiency of kidney-YANG patient except sexuality goes down, main sign be exactly fatiguability, spirit not good, hypoxia-bearing capability difference causes fatiguability, the not good major cause of spirit, and thus raising mouse hypoxia-bearing capability can improve fatiguability, spirit is not good, thus improves insufficiency of kidney-YANG illness.As seen from the results in Table 9, grape wine of the present invention significantly increases mouse hypoxia endurance time, and is better than other 5 groups, shows that it has significant curative effect to fatiguability, the spirit insufficiency of kidney-YANG symptom such as not good.

Embodiment 12: mouse swimming exercise simultaneous test

Test grouping: with embodiment 4 clustering method.

Test method: get healthy male mice in kunming (body weight 20 ± 2 grams) 60, be divided into 6 groups at random, often organize 10, each group gives above-mentioned 4 groups of medicinal liquor and physiological saline respectively, the heavy gavage of 0.1mL/10g mouse, every day 1 time, continuous 7 days, fasten 2 grammes per square metre little iron ring at each mouse tail line in the 8th day gavage after 1 hour, mouse is put into the bucket of depth of water 20cm, timing immediately, sinks under water completely no longer till upstream until mouse, the results are shown in Table 10.

Table 10 mouse swimming exercise result ( n=10)

Group	Swimming time (min)
		Physiological saline group	19.3±1.5
Grape wine group	21.5±1.2
		Embodiment 1 group	26.7±2.3
Strength wine group	19.5±1.4
		Three animal penes wine group	19.3±2.1
Deer antler wine group	19.6±1.9

Sportive efforts endurance is a reaction health energy whether vigorous important indicator, be also that the middle energy of insufficiency of kidney-YANG is not good, fatiguability, direct performance slow in reacting etc., thus improve exercise tolerance and muscle power is also the improved direct embodiment of insufficiency of kidney-YANG.As seen from the results in Table 10, grape wine of the present invention significantly increases mouse swimming time, and is better than other 5 groups, shows that it is not good to energy, fatiguability, the insufficiency of kidney-YANG symptom such as slow in reacting have significant curative effect.

Embodiment 13: to CAMP, E2, T, Cu in deficiency of the kidney yin mice plasma ⁺⁺, Zn ⁺⁺the impact of content

Test grouping: Normal group, Kidney-yin-vacuity control group, commercially available three animal penes wine group (lot identification mark: 20100312), commercially available deer antler wine group (lot identification mark: 20100619), commercial glucose wine group (lot identification mark 20100813), embodiment 1 group, commercially available strength wine group (lot identification mark 20100821).

Test method: Kunming mouse 450, male and female half and half, body weight (20 ± 2) g, purchased from Medical University Of Chongqing's Experimental Animal Center.Fasting 12h before experiment, freely drinks water.Laboratory temperature (24 ± 2) DEG C, humidity 60%.Model mice subcutaneous injection hydrocortisone 1mg/ only, the next day 1 time, totally 3 times, present deficiency of the kidney yin symptom.The capacity physiological saline such as Normal group injection.2h after injection, by following grouping perfusion respectively.Normal group and Kidney-yin-vacuity control group (physiological saline 20ml/kg); Grape wine group (20ml/kg); Embodiment 1 group (20ml/kg); Strength wine group (20ml/kg); Three animal penes wine group (20ml/kg); Deer antler wine group (20ml/kg).Each treated animal gastric infusion every day once, continuous 7 days.Broken end gets blood, separated plasma, with measured by radioimmunoassay CAMP, E2, T content, uses aas determination Cu ⁺⁺, Zn ⁺⁺content, the results are shown in Table 11.

Each group of table 11 is to CAMP, E2, T, Cu in mice plasma ⁺⁺, Zn ⁺⁺content influence ( n=10)

Note: * represents that embodiment 1 group has significant difference P < 0.05 compared with Normal group

Table 11 result is known, takes CAMP, E2, Cu after grape wine of the present invention ⁺⁺, Zn ⁺⁺content obviously reduces, T content obviously raises, and show that grape wine of the present invention has obvious curative effects to deficiency of the kidney yin mouse, significant difference is remarkable.

Embodiment 14: the impact of deficiency of the kidney yin mouse being engulfed to phagocyte imdex, test for humoral immunity

Test grouping: with embodiment 13 clustering method.

Test method: obtain deficiency of the kidney yin mouse model by the method for embodiment 13.

By following grouping perfusion respectively.Normal group and Kidney-yin-vacuity control group (physiological saline 20ml/kg); Grape wine group (20ml/kg); Embodiment 1 group (20ml/kg); Strength wine group (20ml/kg); Three animal penes wine group (20ml/kg); Deer antler wine group (20ml/kg).Each treated animal gastric infusion every day once, continuous 7 days.

1, carbon granules test is engulfed: get male mice 70, be divided into 7 groups at random, often organize 10,2h after last administration, (stoste is centrifugal with front 2000rmp for tail vein injection 50% india ink 0.1ml/10g, discard throw out, 2 times are diluted with 0.9%N.S) 2min, 10min afterwards, get blood 25ul from eyeground respectively, be dissolved in 2ml distilled water, with normal mouse blood for blank, survey absorbancy (OD) at 72 type spectrophotometer 600nm wavelength places.Index K is cleaned up in calculating, K=1/8 × lg (OD ₂/ OD ₁₀), OD ₂, OD ₁₀be respectively 2min and 10min OD value.

2, test for humoral immunity: get male mice 70, be divided into 7 groups at random, often organize 10, administration mouse peritoneal on the 1st is injected 5% sheep red blood cell (SRBC) (SRBC) 0.2ml and is carried out initial immunity, within the 5th day after immunity, extracts eyeball and gets blood, separation of serum.Get 1:5000 dilute serum 1ml, 5%SRBC0.5ml, guinea pig serum 1ml, mixing, blank pipe replaces mice serum with NS, and other is the same.37 DEG C of water-bath 10min, after water-bath termination reaction, the centrifugal 10min of 2000rmp, gets supernatant liquor 1ml, adds Dou Shi reagent 3.25ml red corpuscle HD50 control tube, gets SRBC 0.25ml, add 4ml Dou Shi reagent, fully shake up.Place 10min, in 540nm place, measure each pipe absorbance respectively.Calculate half hemolysis value (HC50).

Half hemolysis value=sample light absorption value/SRBC HD50 light absorption value * extension rate

Statistical study adopts NDST, and measurement data t checks, and enumeration data X2 checks, and analyzes the significance between each group, and contrasts between two, and significance level is P=0.05.Result is as shown in table 12:

Table 12 is cleaned up exponential sum and is calculated HD50 Data-Statistics after respectively organizing medication

Group	Dosage (ml/kg)	Clean up index (× 10 ^-3)	HC50
				Normal group	20	19.81±9.35	318.76±48.71
Model group	20	6.59±2.53	97.89±20.91
				Grape wine group	20	15.36±5.61	171.37±21.45
Embodiment 1 group	20	22.75±11.68	285.61±36.39
				Strength wine group	20	14.19±5.36	154.39±17.29
Three animal penes wine group	20	27.61±7.98	194.21±32.60
				Deer antler wine group	20	44.78±11.34	255.26±47.83

From table 12 result, deficiency of the kidney yin mouse engulfs phagocyte imdex and HC50 obviously reduces, and grape wine of the present invention obviously can strengthen deficiency of the kidney yin mouse phagocyte imdex and antibody-producing capacity, than commercially available health promoting wine and grape wine Be very effective.

Embodiment 15: preparation method of the present invention prepares grape wine

Take 100g matrimony vine, 100g Poria cocos, 100g Caulis arachidis hypogaeae, 50g sealwort, 25g Chinese cassia tree, 50g mulberry fruit respectively, pulverized 14 object sieves, and loaded in good Buddhist nun's virtue fermentor tank.Get 34Kg fresh grape (Merlot, Cabernet Sauvignon etc.) in addition, Whitfield's ointment (SA) is first adopted to spray process, per kilogram grape sprays 100mlSA solution, and the suitable concentration Merlot that SA sprays is 100mg/L, Cabernet Sauvignon is 60mg/L, deposits 72h after process in darkroom; Taking-up SA sprays the grape after process, and with the synthesis from veratryl alcohol in ultraviolet (UV) radiation-induced grape, UV induces optimal dose Merlot to be 430 seconds (1.28KJ/m ²), Cabernet Sauvignon is 310 seconds (0.9KJ/m ²), deposit 72h in darkroom after process.Take out grape that UV irradiates first with ortho-water cleaning, then use washed with de-ionized water three times, grape harvest by hand or destemming crusher remove carpopodium, the fragmentation of fruit grain is obtained grape slurry, adds in good Buddhist nun's virtue fermentor tank; In the grape slurry of fragmentation, add sulfurous acid 0.117%, make sulfur dioxide concentration reach 0.007%, add 8.5kg sucrose, stir.Adjustment Sucus Vitis viniferae sugar degree is 25%, and acid content is 0.6%, the saccharomyces bayanus (fermenting power (CO of inoculation 0.3wt% ₂) > 950ml moisture content < 5%) and 0.3wt%HE GRAND CRU polygalacturonase (enzyme activity 400,000) and 0.3wt%-0.5wt% glucuroide (enzyme activity 100,000) ferment, leavening temperature is controlled below 28-30 DEG C between yeast phase, every day stirs 2-4 time, makes the medicinal powder of floating and skin slag be pressed in Sucus Vitis viniferae; The 8 days secondary fermentation liquid residual sugars that ferment are down to less than 0.06%, and broth temperature controls in room temperature, filter wine liquid separation skin slag and obtain original wine; The sulfurous gas adding 0.3% in original wine carries out secondary fermentation, and wine is filled liquor storage vessel sealing and preserved, and temperature controls at 18-25 DEG C, and period carries out malic-lactic acid fermentation as required; Then control temperature carries out ageing at 10-15 DEG C, relative humidity 85%-90%, and carries out down bucket, bucketful and clarifying treatment; Measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product and carry out blending process, store 28 days; Wine liquid is crossed 0.45 μm of filter membrane and carry out essence filter, pass into sterilizer 90 DEG C/min quick sterilization, bottling sealing, in the water of 70-72 DEG C, sterilization 20min, is up to the standards and gets final product code, vanning, warehouse-in.

Carry out correlation detection according to the method for embodiment 2, embodiment 6-14, the grape wine prepared of the present embodiment is to the insufficiency of kidney-YANG symptom such as impotence, sexual hypofunction, fatiguability, hypoimmunity, energy is not good and have significant curative effect, and effect is better than existing healthcare product.

Embodiment 16: preparation method of the present invention prepares grape wine

Take 60g matrimony vine, 40g Poria cocos, 80g Caulis arachidis hypogaeae, 50g sealwort, 25g Chinese cassia tree, 45g mulberry fruit respectively, pulverized 14 object sieves, and loaded in good Buddhist nun's virtue fermentor tank.

Get 20Kg fresh grape (Merlot, Cabernet Sauvignon etc.) in addition, Whitfield's ointment (SA) is first adopted to spray process, per kilogram grape sprays 100mlSA solution, and the suitable concentration Merlot that SA sprays is 100mg/L, Cabernet Sauvignon is 60mg/L, deposits 72h after process in darkroom; Taking-up SA sprays the grape after process, and with the synthesis from veratryl alcohol in ultraviolet (UV) radiation-induced grape, UV induces optimal dose Merlot to be 430 seconds (1.28KJ/m ²), Cabernet Sauvignon is 310 seconds (0.9KJ/m ²), deposit 72h in darkroom after process.Take out the grape that UV irradiates, first with ortho-water cleaning, then use washed with de-ionized water three times, grape harvest by hand or destemming crusher remove carpopodium, the fragmentation of fruit grain is obtained grape slurry, adds in good Buddhist nun's virtue fermentor tank.

In the grape slurry of fragmentation, add sulfurous acid 0.066%, make sulfur dioxide concentration reach 0.004%, add 4kg sucrose, stir.Adjustment Sucus Vitis viniferae sugar degree is 20%, and acid content is 0.6%, the saccharomyces bayanus (fermenting power (CO of inoculation 0.5wt% ₂) > 950ml moisture content < 5%) and 0.5wt%HE GRAND CRU polygalacturonase (enzyme activity 400,000) and 0.5wt%-0.5wt% glucuroide (enzyme activity 100,000) ferment, leavening temperature is controlled below 28-30 DEG C between yeast phase, every day stirs 2-4 time, makes the medicinal powder of floating and skin slag be pressed in Sucus Vitis viniferae; The 9 angel's fermented liquid residual sugars that ferment are down to less than 0.06%, and broth temperature controls in room temperature, filter wine liquid separation skin slag and obtain original wine; The sulfurous gas adding 0.3% in original wine carries out secondary fermentation, and wine is filled liquor storage vessel sealing and preserved, and temperature controls at 18-25 DEG C, and period carries out malic-lactic acid fermentation as required; Then control temperature carries out ageing at 10-15 DEG C, relative humidity 85%-90%, and carries out down bucket, bucketful and clarifying treatment; Measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product and carry out blending process, store 28 days; Wine liquid is crossed 0.45 μm of filter membrane and carry out essence filter, pass into sterilizer 90 DEG C/min quick sterilization, bottling sealing, in the water of 70-72 DEG C, sterilization 20min, is up to the standards and gets final product code, vanning, warehouse-in.

Carry out correlation detection according to the method for embodiment 2, embodiment 6-14, to impotence, sexual hypofunction, fatiguability, hypoimmunity, energy is not good etc., insufficiency of kidney-YANG symptom has significant curative effect to the grape wine prepared of the present embodiment, and effect is better than existing healthcare product.

Embodiment 17: preparation method of the present invention prepares grape wine

Take 100g matrimony vine, 75g Poria cocos, 80g Caulis arachidis hypogaeae, 70g sealwort, 35g Chinese cassia tree, 60g mulberry fruit respectively, pulverized 14 object sieves, and loaded in good Buddhist nun's virtue fermentor tank.

Get 24Kg fresh grape (Merlot, Cabernet Sauvignon etc.) in addition, Whitfield's ointment (SA) is first adopted to spray process, per kilogram grape sprays 100mlSA solution, and the suitable concentration Merlot that SA sprays is 100mg/L, Cabernet Sauvignon is 60mg/L, deposits 72h after process in darkroom; Taking-up SA sprays the grape after process, and with the synthesis from veratryl alcohol in ultraviolet (UV) radiation-induced grape, UV induces optimal dose Merlot to be 430 seconds (1.28KJ/m ²), Cabernet Sauvignon is 310 seconds (0.9KJ/m ²), deposit 72h in darkroom after process.Take out the grape that UV irradiates, first with ortho-water cleaning, then use washed with de-ionized water three times, grape harvest by hand or destemming crusher remove carpopodium, the fragmentation of fruit grain is obtained grape slurry, adds in good Buddhist nun's virtue fermentor tank.

In the grape slurry of fragmentation, add sulfurous acid 0.1%, make sulfur dioxide concentration reach 0.006%, add 5.2kg sucrose, stir.Adjustment Sucus Vitis viniferae sugar degree is 22%, and acid content is 0.6%, the saccharomyces bayanus (fermenting power (CO of inoculation 0.3wt% ₂) > 950ml moisture content < 5%) and 0.3wt%HE GRAND CRU polygalacturonase (enzyme activity 400,000) and 0.3wt%-0.5wt% glucuroide (enzyme activity 100,000) ferment, leavening temperature is controlled below 28-30 DEG C between yeast phase, every day stirs 2-4 time, makes the medicinal powder of floating and skin slag be pressed in Sucus Vitis viniferae; The 9 days fermented liquid residual sugars that ferment are down to less than 0.06%, and broth temperature controls in room temperature, filter wine liquid separation skin slag and obtain original wine; The sulfurous gas adding 0.3% in original wine carries out secondary fermentation, and wine is filled liquor storage vessel sealing and preserved, and temperature controls at 18-25 DEG C, and period carries out malic-lactic acid fermentation as required; Then control temperature carries out ageing at 10-15 DEG C, relative humidity 85%-90%, and carries out down bucket, bucketful and clarifying treatment; Measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product and carry out blending process, store 28 days; Wine liquid is crossed 0.45 μm of filter membrane and carry out essence filter, pass into sterilizer 90 DEG C/min quick sterilization, bottling sealing, in the water of 70-72 DEG C, sterilization 20min, is up to the standards and gets final product code, vanning, warehouse-in.

Embodiment 18: preparation method of the present invention prepares grape wine

Take 160g matrimony vine, 110g Poria cocos, 130g Caulis arachidis hypogaeae, 120g sealwort, 60g Chinese cassia tree, 120g mulberry fruit respectively, pulverized 14 object sieves, and loaded in good Buddhist nun's virtue fermentor tank.

Get 56Kg fresh grape (Merlot, Cabernet Sauvignon etc.) in addition, Whitfield's ointment (SA) is first adopted to spray process, per kilogram grape sprays 100mlSA solution, and the suitable concentration Merlot that SA sprays is 100mg/L, Cabernet Sauvignon is 60mg/L, deposits 72h after process in darkroom; Taking-up SA sprays the grape after process, and with the synthesis from veratryl alcohol in ultraviolet (UV) radiation-induced grape, UV induces optimal dose Merlot to be 430 seconds (1.28KJ/m ²), Cabernet Sauvignon is 310 seconds (0.9KJ/m ²), deposit 72h in darkroom after process.Take out the grape that UV irradiates, first with ortho-water cleaning, then use washed with de-ionized water three times, grape harvest by hand or destemming crusher remove carpopodium, the fragmentation of fruit grain is obtained grape slurry, adds in good Buddhist nun's virtue fermentor tank.

In the grape slurry of fragmentation, add sulfurous acid 0.066%, make sulfur dioxide concentration reach 0.004%, add 10.08kg sucrose, stir.Adjustment Sucus Vitis viniferae sugar degree is 18%, and acid content is 0.6%, the saccharomyces bayanus (fermenting power (CO of inoculation 0.3wt% ₂) > 950ml moisture content < 5%) and 0.3wt%HE GRANDCRU polygalacturonase (enzyme activity 400,000) and 0.3wt%-0.5wt% glucuroide (enzyme activity 100,000) ferment, leavening temperature is controlled below 28-30 DEG C between yeast phase, every day stirs 2-4 time, makes the medicinal powder of floating and skin slag be pressed in Sucus Vitis viniferae; The 9 days secondary fermentation liquid residual sugars that ferment are down to less than 0.06%, and broth temperature controls in room temperature, filter wine liquid separation skin slag and obtain original wine; The sulfurous gas adding 0.3% in original wine carries out secondary fermentation, and wine is filled liquor storage vessel sealing and preserved, and temperature controls at 18-25 DEG C, and period carries out malic-lactic acid fermentation as required; Then control temperature carries out ageing at 10-15 DEG C, relative humidity 85%-90%, and carries out down bucket, bucketful and clarifying treatment; Measure pol, wine degree, the acidity of original wine, adjust to prescribed value respectively according to the physical and chemical index of finished product and carry out blending process, store 28 days; Wine liquid is crossed 0.45 μm of filter membrane and carry out essence filter, pass into sterilizer 90 DEG C/min quick sterilization, bottling sealing, in the water of 70-72 DEG C, sterilization 20min, is up to the standards and gets final product code, vanning, warehouse-in.

Embodiment 19: clinical trial

1, slowly certain, man, 40 years old, to be consciously at ordinary times short of physical strength, impotence is lifted, and breathes hard, manual work lethargy.Through tcm diagnosis for for suffering from a deficiency of the kidney.Drink grape wine described in the embodiment of the present invention 1 to carry out taking good care of and treating, every day drinks 50 milliliters after supper, drinks 3 months continuously, and muscle power is restored, energetic, impotence transference cure, supports and hard.Think after TCM pulse diagnosis, its renal function reaches the same with normal people.Confirm that its illness obtains medical treatment, muscle power and energy are restored.Prove that grape wine of the present invention is to suffering from a deficiency of the kidney effectively.

2, Lee, man, 38 years old, conscious dampness of scrotum, the empty hidrosis of body, erected unfavorable.Think through tcm diagnosis, insufficiency of kidney-YANG.Drink grape wine described in the embodiment of the present invention 1 to nurse one's health, every day 1 time, each 80 milliliters, drink after supper.Through drinking 45 days, treating shape and disappearing.Scrotum is no longer moist, and vigorous generation, rapidly with strong, consciously feels full of physical strength.Prove the effect that grape wine of the present invention creates treatment and regulates.

3, Zhu, man, 47 years old, conscious weak all over, breathe hard, night sweat.Think through tcm diagnosis, caused by suffering from a deficiency of the kidney.Drink grape wine described in the embodiment of the present invention 1 to take good care of.Drink 50 milliliters at every turn, respectively drink 1 time after every day supper and before sleep, drink every day with 100 milliliters.After drinking through 2 months, from head to foot effectively, phenomenon of breathing hard disappear, night sweat stop to.Prove that grape wine of the present invention creates the effect of conditioning.

4, Lee, man, 52 years old, conscious thanks to gas, head fan, impotence, little energy having sexual intercourse thing.Think through tcm diagnosis, seriously suffer from a deficiency of the kidney and held back.Drink grape wine described in the embodiment of the present invention 1 carry out treating and nurse one's health.Drink 50 milliliters every day, drink after supper, after drinking 3 months, feel and no longer lose gas, vital capacity increases, head fan transference cure, no longer impotence, and during having sexual intercourse, positive tool is powerful and vigorous, proves that grape wine of the present invention creates treatment and opsonization.

5, money, man, 56 years old, having sexual intercourse difficulty, impotence sometimes, sometimes premature ejaculation, perplexed its orthobiosis often.Think insufficiency of kidney-YANG through tcm diagnosis, sexual disorder, suggestion kidney tonifying method is treated.To drink described in the embodiment of the present invention 15 grape wine every day 60 milliliters, after drinking 2 months, shut out certain with a smile in whereabouts doctor explain the situation, say that having sexual intercourse is as young man, is in a cheerful frame of mind.Prove that grape wine of the present invention weakens successful to treatment man sexual function.

6, Zhao, man, 63 years old, within 2006, suffer from sexual dysfunction, with suffering from a deficiency of the kidney, soreness of the waist and knees illness, through hospitalize poor effect, start to drink this grape wine from October, 2008, after half a year, the state of an illness takes a turn for the better gradually, after this adheres to drinking grape wine described in the embodiment of the present invention 16 50 milliliters every night, so far more than 4 years, not only cure and suffered from a deficiency of the kidney and the disease such as sexual dysfunction, and agerasia, hale and hearty, look as 50 how old people.

7, Lee, man, 42 years old, suffers from night sweat, lumbago due to renal deficiency, the not normal illness of sexual function, and control little more for a long time, bad during fashion, take grape wine described in the embodiment of the present invention 16 and be clearly better after 3 months, return to one's perfect health after half a year, still adhering to taking at present, physique is become better and better.

8, river, man, 49 years old, suffer from deficiency of the liver and kindey, waist knee was weak, blockage of main and collateral channels illness.In poor health, lassitude, very micro-through in many ways curing curative effect.In May, 2009 starts to take grape wine described in the embodiment of the present invention 17, and October, sb.'s illness took a favorable turn, and the first half of the year in 2010 is almost recovered, and clothes are to now, and physical appearance is good.

9, certain is shut out, man, 60 years old, after patient tumbles for 2008 by motorcycle, waist bar is sprained and is not fully recovered always, and often show pain in the back, find that tumble injury position goes out the obvious hyperosteogeny of item in November, 2010, its trick in winter is ice-cold, chilly is afraid of cold, the tired power of waist, insomnia and dreamful sleep, impotence erectile problem.Rise on August 26th, 2011 and start to take grape wine described in the embodiment of the present invention 17,70 milliliters are taken every night during supper, after serveing on 15 days, pain in the back obviously alleviates, and takes 45 days continuously afterwards, pain in the back completely dissolve, hyperosteogeny is obviously eliminated to go hospital's photo to find, sleep is good and dream is few, and trick is warmed up, and body is strengthened, energetic, positive tool is sturdy strong in 30 years old young man.

10, Zheng, female, 52 years old, suffers from rheumatism, similar rheumatism, sacroiliitis, whenever raining, all can knee pain when the change of weather, very micro-through in many ways curing curative effect.In October, 2010 starts to take grape wine described in the embodiment of the present invention 18, connects drink 15 days, and painful area has and obviously alleviates, and drink 2 months continuously, during drinking, disease sites is without obvious pain.

11, Huang, female, 47 years old, suffer from frequent micturition, urgent urination, the symptom such as frequently to get up in the night to urinate, take grape wine 7-15 days described in the embodiment of the present invention 18, frequency of getting up in the night to urinate obviously reduces, and urine urgency-frequency is also improved, and connects drink 1-2 month, without getting up in the night to urinate symptom, does not also rebound after stopping drinking.

12, wear certain, female, 36 years old, suffer from soreness of the waist and knees, two skelasthenias, dizzy tinnitus, insomnia and dreamful sleep, originally thinking what oneself each side's pressure caused greatly, is deficiency of the kidney yin through tcm diagnosis.Take grape wine 15-25 days described in the embodiment of the present invention 18, physique is obviously improved, and sleep quality significantly improves, and connect drink more than 2 months, illness does not occur substantially, and the symptom that also do not rebound during stopping drink occurs.

13, for certain, female, 28 years old, patient's warp was few, emaciated physique, hectic fever night sweat.Take grape wine described in the embodiment of the present invention 18 15 days-1 month (stopping using during menstrual period), symptom has clear improvement, and connects drink 3 months, and occur without illness, physique is also significantly improved and improves.

The explanation of above embodiment just understands method of the present invention and core concept thereof for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.

Claims

1. a grape wine, be made up of following raw material:

Matrimony vine 50 parts-100 parts, 40 parts-100 parts, Poria cocos, Caulis arachidis hypogaeae 50 parts-100 parts, sealwort 50 parts-70 parts, Chinese cassia tree 25 parts-50 parts, 45 parts-60 parts, mulberry fruit, grape 20000 parts-34000 parts.

2. a preparation of wine, the 20000 pretreated grapes of weight part-34000 weight part and 50 weight part-100 weight part matrimony vines, 40 weight part-100 weight part Poria cocos, 50 weight part-100 weight part Caulis arachidis hypogaeaes, 50 weight part-70 weight part sealworts, 25 weight part-50 weight part Chinese cassia trees, 45 weight part-60 weight part mulberry fruits are mixed into row zymamsis, after allocate after secondary fermentation, ageing;

3. preparation method according to claim 2, described grape is Merlot, Cabernet Sauvignon.

4. preparation method according to claim 2, described pre-treatment is the synthesis of inducing trans-resveratrol in grape after adopting Whitfield's ointment to spray process with uviolizing.

5. preparation method according to claim 4, described SA concentration is 60mg/L-100mg/L, described UV inducing amount 0.9KJ/m ²-1.28KJ/m ², 310 seconds-430 seconds.

6. preparation method according to claim 2, described zymamsis is 18%-25% for adjusting Sucus Vitis viniferae sugar degree, acid content is 0.6%-0.8%, inoculate the yeast of 0.3wt%-0.5wt%, 0.3wt%-0.5wt% polygalacturonase and 0.3wt%-0.5wt% glucuroide to ferment, control leavening temperature between yeast phase below 28-30 DEG C, ferment to fermented liquid residual sugar and be down to less than 0.06%.

7. preparation method according to claim 6, described yeast is saccharomyces bayanus.

8. preparation method according to claim 6, described polygalacturonase is HE GRAND CRU polygalacturonase.

9. the grape wine that obtains of preparation method described in claim 2-8.

10. the purposes in the medicine of suffering from a deficiency of the kidney treated by grape wine described in claim 1 and claim 9 in preparation.