CN102305781A - Device for detecting ship domestic sewage - Google Patents
Device for detecting ship domestic sewage Download PDFInfo
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- CN102305781A CN102305781A CN201110222933A CN201110222933A CN102305781A CN 102305781 A CN102305781 A CN 102305781A CN 201110222933 A CN201110222933 A CN 201110222933A CN 201110222933 A CN201110222933 A CN 201110222933A CN 102305781 A CN102305781 A CN 102305781A
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Abstract
The invention discloses a device for detecting ship domestic sewage, comprising a microfluidic chip, a fluorescent detection unit, a signal processing unit and a display unit, wherein the microfluidic chip comprises a microfluidic channel made of a light transmission material and is used for being flown through by a sample solution containing fluorescent dyed bacteria; the fluorescent detection unit is used for giving out a laser to the microfluidic channel and receiving fluorescence generated after the bacteria are excited by the laser; the fluorescence received by the fluorescent detection unit is transformed into an electric signal and is amplified by the signal processing unit; and the display unit is used for counting and displaying the electric signal transformed by the signal processing unit. The detection device of ship domestic sewage provided by the invention can be used for realizing automatic detection on the ship domestic sewage, thus the problems of visual inspection counting by virtue of manual work, long time consumption and big error can be solved; and the detection device has simplicity in operation and high precision and has important social benefit and economic benefit in a maritime affair quantization law enforcement process.
Description
Technical field
The invention belongs to vessel sewage detection technique field, relate in particular to a kind of Sewage from Ships pick-up unit.
Background technology
Sewage from Ships is meant the daily life draining that comes from the ship's staff, and a large amount of pathogenic bacteria that contain in its water body have brought serious threat for the Environmental security of harbour state, and the problem of environmental pollution that it causes just progressively receives the attention of countries in the world.
Therefore, MEPC.2 (V1) that International Maritime Organization (IMO) passed through in 1976 resolution and in revision in 2006 has been carried out strict regulation to Escherichia coli in the sewage etc., and for example, the coliform geometrical mean requires less than 100/100ml etc.
The Sewage from Ships detection mode that prior art provides is that the water body that will collect carries out medium culture, and examines under a microscope, and estimates counting through manual work, and its length consuming time, error are big, can't widespread use in reality.
Summary of the invention
The purpose of the embodiment of the invention is to provide a kind of Sewage from Ships pick-up unit; The Sewage from Ships detection mode that provides with the solution prior art is that the water body that will collect carries out medium culture; And examine under a microscope; Estimate counting through manual work, length consuming time, the problem that error is big.
The embodiment of the invention is achieved in that a kind of Sewage from Ships pick-up unit, and said device comprises:
Confession contains the micro-fluidic chip that the sample solution of fluorescent dye bacterium flows through, and said micro-fluidic chip comprises the fluid channel that a light-transmitting materials forms;
Send laser to said fluid channel, and receive the fluorescence detection unit of the fluorescence that bacterium in the said sample solution produces after by said laser excitation;
The fluorescence that said fluorescence detection unit is received converts electric signal to and the electric signal after the conversion is carried out the signal processing unit of processing and amplifying;
The display unit that connects said signal processing unit, the electric signal after the conversion of said signal processing unit is counted and shown.
In the above-mentioned Sewage from Ships pick-up unit, said fluid channel can be the fluid channel of being processed by poly dimethyl oxosilane or polymethylmethacrylate.
In the above-mentioned Sewage from Ships pick-up unit, said fluid channel further can comprise entrance channel, contracted channel and outlet flow along the direction of inlet-outlet; The internal diameter of said entrance channel and the internal diameter of said outlet flow are all greater than the internal diameter of said contracted channel.
In the above-mentioned Sewage from Ships pick-up unit, said micro-fluidic chip can also comprise:
Place said outlet flow end, drive the power unit that said sample solution flows in said fluid channel.
In the above-mentioned Sewage from Ships pick-up unit, said fluorescence detection unit can comprise:
Send the laser instrument of laser;
First optical filter is used for the parasitic light of the said laser that the said laser instrument of filtering sends;
Dichroic mirror is used to reflect the laser after said first optical filter filters;
First convergent lens is used for the laser of said dichroic mirror reflects is gathered in said contracted channel, and the said fluorescence that said contracted channel sends is gathered in said dichroic mirror;
Second optical filter is used for filtering and disturbs through the laser of the said fluorescence of said dichroic mirror transmission;
Second convergent lens is used for the said fluorescence after said second optical filter optical filtering is gathered in said signal processing unit.
In the above-mentioned Sewage from Ships pick-up unit, said signal processing unit can comprise:
Modular converter is used for converting the said fluorescence that said second convergent lens is assembled into electric signal;
The signal amplification module is used for the said electric signal after the said modular converter conversion is carried out processing and amplifying;
Counting module after being used for the electric signal after the said signal amplification module processing and amplifying counted, is exported to said display unit.
Use Sewage from Ships pick-up unit provided by the invention and can realize automatic detection, avoided existing and estimated counting through manual work to Sewage from Ships, length consuming time, the problem that error is big, and simple to operate, precision is high; Signal processing unit wherein and display unit can be integrated in single-chip microcomputer or the handheld device, so that device volume reduces, are easy to carry, and quantize to have great social significance and economic worth in the enforcing law in maritime affairs.
Description of drawings
Fig. 1 is the structure principle chart of Sewage from Ships pick-up unit provided by the invention;
Fig. 2 is the structural drawing of micro-fluidic chip and fluorescence detection unit among Fig. 1;
Fig. 3 is the structural drawing of signal processing unit among Fig. 1.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
Fig. 1 shows the structural principle of Sewage from Ships pick-up unit provided by the invention.
Sewage from Ships pick-up unit provided by the invention comprises: supply to contain the micro-fluidic chip 11 that the sample solution of fluorescent dye bacterium flows through, it comprises the fluid channel that a light-transmitting materials forms; Send laser to fluid channel, and receive the fluorescence detection unit 12 of the fluorescence that bacterium in the sample solution produces after by this laser excitation; The fluorescence that fluorescence detection unit 12 is received converts electric signal to and the electric signal after the conversion is carried out the signal processing unit 13 of processing and amplifying; The display unit 14 that connects signal processing unit 13, the electric signal after signal processing unit 13 conversions is counted and shown.
Use Sewage from Ships pick-up unit provided by the invention and can realize automatic detection, avoided existing and estimated counting through manual work to Sewage from Ships, length consuming time, the problem that error is big, and simple to operate, precision is high; Signal processing unit 13 wherein can be integrated in single-chip microcomputer or the handheld device with display unit 14, so that device volume reduces, is easy to carry, and quantizes to have great social significance and economic worth in the enforcing law in maritime affairs.
Light-transmitting materials wherein is preferably runner or the polymethylmethacrylate (PMMA) that poly dimethyl oxosilane (PDMS) is processed;
Fig. 2 shows the structure of micro-fluidic chip 11 among Fig. 1 and fluorescence detection unit 12.
Wherein, the fluid channel of micro-fluidic chip 11 further comprises along the direction of inlet-outlet: entrance channel 111, contracted channel 112, outlet flow 114; And the internal diameter of the internal diameter of entrance channel 111 and outlet flow 114 is all greater than the internal diameter of contracted channel 112.Micro-fluidic chip 11 also comprises: place outlet flow 114 ends, drive the power unit 113 that sample solution flows in fluid channel.
Wherein, fluorescence detection unit 12 comprises: the laser instrument 126 that sends laser; First optical filter 125 is used for the parasitic light of the laser that filtering laser instrument 126 sends; Dichroic mirror 122 is used to reflect the laser after first optical filter 125 filters; First convergent lens 121 is used for dichroic mirror 122 laser light reflected are gathered in contracted channel 112, and the fluorescence that contracted channel 112 sends is gathered in dichroic mirror 122; Second optical filter 123 is used for filtering and disturbs through the laser of the fluorescence of dichroic mirror 122 transmissions; Second convergent lens 124 is used for the fluorescence after 123 optical filterings of second optical filter is gathered in signal processing unit 13.
Fig. 3 is the structure of signal processing unit 13 among Fig. 1.
Use Sewage from Ships pick-up unit provided by the invention and can realize automatic detection, avoided existing and estimated counting through manual work to Sewage from Ships, length consuming time, the problem that error is big, and simple to operate, precision is high; Signal processing unit 13 wherein can be integrated in single-chip microcomputer or the handheld device with display unit 14, so that device volume reduces, is easy to carry, and quantizes to have great social significance and economic worth in the enforcing law in maritime affairs.
The above; Only be the preferable embodiment of the present invention; But protection scope of the present invention is not limited thereto; Any technician who is familiar with the present technique field is in the technical scope that the present invention discloses; Be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, all should be encompassed within protection scope of the present invention.
Claims (6)
1. a Sewage from Ships pick-up unit is characterized in that, said device comprises:
Confession contains the micro-fluidic chip that the sample solution of fluorescent dye bacterium flows through, and said micro-fluidic chip comprises the fluid channel that a light-transmitting materials forms;
Send laser to said fluid channel, and receive the fluorescence detection unit that bacterium in the said sample solution produces fluorescence after by said laser excitation;
The fluorescence that said fluorescence detection unit is received converts electric signal to and the electric signal after the conversion is carried out the signal processing unit of processing and amplifying;
The display unit that connects said signal processing unit, the electric signal after the conversion of said signal processing unit is counted and shown.
2. Sewage from Ships pick-up unit as claimed in claim 1 is characterized in that, said fluid channel is the fluid channel of being processed by poly dimethyl oxosilane or polymethylmethacrylate.
3. Sewage from Ships pick-up unit as claimed in claim 1 is characterized in that, said fluid channel further comprises entrance channel, contracted channel and outlet flow along the direction of inlet-outlet; The internal diameter of said entrance channel and the internal diameter of said outlet flow are all greater than the internal diameter of said contracted channel.
4. Sewage from Ships pick-up unit as claimed in claim 3 is characterized in that, said micro-fluidic chip also comprises:
Place said outlet flow end, drive the power unit that said sample solution flows in said fluid channel.
5. Sewage from Ships pick-up unit as claimed in claim 3 is characterized in that, said fluorescence induction detecting unit comprises:
Send the laser instrument of laser;
First optical filter is used for the parasitic light of the said laser that the said laser instrument of filtering sends;
Dichroic mirror is used to reflect the laser after said first optical filter filters;
First convergent lens is used for the laser of said dichroic mirror reflects is gathered in said contracted channel, and the said fluorescence that said contracted channel sends is gathered in said dichroic mirror;
Second optical filter is used for filtering and disturbs through the laser of the said fluorescence of said dichroic mirror transmission;
Second convergent lens is used for the said fluorescence after said second optical filter optical filtering is gathered in said signal processing unit.
6. Sewage from Ships pick-up unit as claimed in claim 5 is characterized in that, said signals collecting and processing unit comprise:
Modular converter is used for converting the said fluorescence that said second convergent lens is assembled into electric signal;
The signal amplification module, be used for after the conversion of said modular converter said electric signal carry out processing and amplifying;
Counting module after being used for the electric signal after the said signal amplification module processing and amplifying counted, is exported to said display unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110222933A CN102305781A (en) | 2011-08-04 | 2011-08-04 | Device for detecting ship domestic sewage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110222933A CN102305781A (en) | 2011-08-04 | 2011-08-04 | Device for detecting ship domestic sewage |
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| CN102305781A true CN102305781A (en) | 2012-01-04 |
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| CN201110222933A Pending CN102305781A (en) | 2011-08-04 | 2011-08-04 | Device for detecting ship domestic sewage |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103105352A (en) * | 2013-01-28 | 2013-05-15 | 大连海事大学 | Device and method for rapidly detecting surviving unicellular organisms in ship ballast water |
| CN103364382A (en) * | 2013-07-12 | 2013-10-23 | 大连海事大学 | Ship domestic sewage detection device |
| CN104007091A (en) * | 2013-02-26 | 2014-08-27 | 中国科学院天津工业生物技术研究所 | High-throughput detection system for microbe based on droplet microfluidic chip |
| CN105806768A (en) * | 2016-04-28 | 2016-07-27 | 绍兴文理学院 | Sewage laser test table |
| CN106610415A (en) * | 2016-12-28 | 2017-05-03 | 安徽壹诺环境工程有限公司 | Soil and underground water monitoring system and method |
| CN109374584A (en) * | 2018-08-27 | 2019-02-22 | 九江精密测试技术研究所 | A kind of ballast water for ship biological effectiveness real time on-line monitoring device |
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| US4686372A (en) * | 1983-05-09 | 1987-08-11 | Mitsubishi Denki Kabushiki Kaisha | Method and apparatus for measuring cell counts of Methanogens or methane producing activity thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103105352A (en) * | 2013-01-28 | 2013-05-15 | 大连海事大学 | Device and method for rapidly detecting surviving unicellular organisms in ship ballast water |
| CN104007091A (en) * | 2013-02-26 | 2014-08-27 | 中国科学院天津工业生物技术研究所 | High-throughput detection system for microbe based on droplet microfluidic chip |
| CN103364382A (en) * | 2013-07-12 | 2013-10-23 | 大连海事大学 | Ship domestic sewage detection device |
| CN105806768A (en) * | 2016-04-28 | 2016-07-27 | 绍兴文理学院 | Sewage laser test table |
| CN106610415A (en) * | 2016-12-28 | 2017-05-03 | 安徽壹诺环境工程有限公司 | Soil and underground water monitoring system and method |
| CN109374584A (en) * | 2018-08-27 | 2019-02-22 | 九江精密测试技术研究所 | A kind of ballast water for ship biological effectiveness real time on-line monitoring device |
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Application publication date: 20120104 |