WO2013016873A1 - Ship domestic sewage detection device - Google Patents

Ship domestic sewage detection device Download PDF

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Publication number
WO2013016873A1
WO2013016873A1 PCT/CN2011/078008 CN2011078008W WO2013016873A1 WO 2013016873 A1 WO2013016873 A1 WO 2013016873A1 CN 2011078008 W CN2011078008 W CN 2011078008W WO 2013016873 A1 WO2013016873 A1 WO 2013016873A1
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WIPO (PCT)
Prior art keywords
fluorescence
domestic sewage
laser
processing unit
ship domestic
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PCT/CN2011/078008
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French (fr)
Chinese (zh)
Inventor
张洪朋
李冬青
孙野青
黄文�
Original Assignee
Zhang Hongpeng
Li Dongqing
Sun Yeqing
Huang Wen
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Publication date
Application filed by Zhang Hongpeng, Li Dongqing, Sun Yeqing, Huang Wen filed Critical Zhang Hongpeng
Priority to PCT/CN2011/078008 priority Critical patent/WO2013016873A1/en
Publication of WO2013016873A1 publication Critical patent/WO2013016873A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/008—Originating from marine vessels, ships and boats, e.g. bilge water or ballast water
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/36—Biological material, e.g. enzymes or ATP
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/40—Liquid flow rate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/019—Biological contaminants; Fouling

Definitions

  • the invention belongs to the technical field of ship sewage detection, and particularly relates to a ship domestic sewage detecting device. Background technique
  • Ship domestic sewage refers to the daily life drainage of people from the ship.
  • the large amount of pathogenic bacteria contained in the water body poses a serious threat to the environmental safety of the port state.
  • the environmental pollution caused by it is gradually receiving the attention of all countries in the world.
  • the domestic sewage detection method provided by the prior art is that the collected water body is cultured in a medium and observed under a microscope, and the manual measurement and counting are performed manually, which takes a long time and a large error, and cannot be widely applied in practice. Summary of the invention
  • the object of the embodiments of the present invention is to provide a ship domestic sewage detecting device, which solves the problem that the domestic sewage detection method provided by the prior art is that the collected water body is cultured in a medium and observed under a microscope, and the visual counting is performed by manual. , a long time, a large error.
  • a ship domestic sewage detecting device comprising: a microfluidic chip for flowing a sample solution containing fluorescent staining bacteria, wherein the microfluidic chip comprises a light transmissive material Microchannel
  • a fluorescence detecting unit that emits laser light to the microchannel and receives fluorescence of the sample solution in which the bacteria are excited by the laser;
  • a signal processing unit that converts fluorescence received by the fluorescence detecting unit into an electrical signal and amplifies the converted electrical signal
  • the micro flow path may be a micro flow path made of polydimethyl siloxane or polymethyl methacrylate.
  • the micro flow channel may further include an inlet flow path, a contraction flow path, and an outlet flow path in the direction of the inlet-outlet; the inner diameter of the inlet flow path and the inner diameter of the outlet flow path are both It is larger than the inner diameter of the contraction flow path.
  • the microfluidic chip may further include:
  • the fluorescence detecting unit may include:
  • a first filter for filtering stray light in the laser emitted by the laser
  • a dichroic mirror for reflecting the laser light filtered by the first filter
  • a first collecting lens for collecting the laser light reflected by the dichroic mirror in the contracting flow channel, and collecting the fluorescent light emitted by the contracting flow channel to the dichroic mirror;
  • a second filter for filtering laser interference in the fluorescence transmitted through the dichroic mirror; a second collecting lens for collecting the fluorescent light after filtering the second filter In the signal processing unit.
  • the signal processing unit may include:
  • a conversion module configured to convert the fluorescence collected by the second concentrating lens into an electrical signal
  • a signal amplification module configured to perform amplification processing on the electrical signal converted by the conversion module
  • the signal amplification module amplifies the processed electrical signal and outputs it to the display unit.
  • the ship domestic sewage detecting device provided by the invention can realize the automatic detection of the domestic sewage of the ship, avoiding the problems of manual counting and counting, long time and large error, and the operation is simple and the precision is high; wherein the signal processing unit
  • the display unit and the display unit can be integrated into the single chip microcomputer or the handheld device, so that the device volume is reduced and portable, and has important social significance and economic value in the maritime quantitative law enforcement process.
  • 1 is a schematic structural view of a ship domestic sewage detecting device provided by the present invention
  • 2 is a structural view of the microfluidic chip and the fluorescence detecting unit of FIG. 1;
  • FIG. 3 is a structural diagram of the signal processing unit of Figure 1.
  • Fig. 1 shows the structural principle of the ship domestic sewage detecting device provided by the present invention.
  • the ship domestic sewage detecting device comprises: a microfluidic chip 11 for flowing a sample solution containing fluorescent dyeing bacteria, comprising a micro flow channel formed by a light transmissive material; emitting laser light to the micro flow channel, and receiving the sample a fluorescence detecting unit 12 that generates fluorescence after being excited by the laser in the solution; a signal processing unit 13 that converts fluorescence received by the fluorescence detecting unit 12 into an electrical signal and amplifies the converted electrical signal; and connects the signal processing unit 13.
  • a display unit 14 that counts and displays the converted electrical signal by the signal processing unit 13.
  • the ship domestic sewage detecting device provided by the invention can realize the automatic detection of the domestic sewage of the ship, avoiding the problems of manual counting and counting, long time and large error, and the operation is simple and the precision is high; wherein the signal processing unit 13 and the display unit 14 can be integrated in a single-chip computer or a handheld device, so that the device is reduced in size and portable, and has important social and economic value in the maritime quantitative law enforcement process.
  • the light transmissive material is preferably a channel made of polydimethyl siloxane (PDMS) or polymethyl methacrylate (PMMA);
  • Fig. 2 shows the structure of the microfluidic chip 11 and the fluorescence detecting unit 12 of Fig. 1.
  • the micro flow channel of the microfluidic chip 11 further includes an inlet flow channel 111, a contraction flow channel 112, and an outlet flow channel 114 in the direction of the inlet-outlet port; and the inner diameter of the inlet flow channel 111 and the inner diameter of the outlet flow channel 114 are both It is larger than the inner diameter of the contraction flow passage 112.
  • the microfluidic chip 11 further includes a power unit 113 placed at the end of the outlet flow path 114 to drive the sample solution to flow in the micro flow path.
  • the fluorescence detecting unit 12 includes: a laser 126 that emits laser light; a first filter 125 for filtering stray light in the laser light emitted by the laser 126; and a dichroic mirror 122 for reflecting the first filter 125 filtered laser light; a first collecting lens 121 for collecting the laser light reflected by the dichroic mirror 122 in the shrinking flow path 112, and collecting the fluorescent light emitted from the shrinking flow path 112 to the dichroic mirror 122; Filter The light sheet 123 is configured to filter out laser interference in the fluorescence transmitted through the dichroic mirror 122; and the second focusing lens 124 is configured to collect the fluorescence filtered by the second filter 123 in the signal processing unit 13.
  • FIG. 3 is a diagram showing the structure of the signal processing unit 13 of Figure 1.
  • the signal processing unit 13 includes: a conversion module 131, configured to convert the fluorescence collected by the second focusing lens 124 into an electrical signal; a signal amplifying module 132, configured to perform amplification processing on the converted electrical signal of the conversion module 131; The electrical signals used for amplifying the signal amplification module 132 are counted and output to the display unit 14.
  • the ship domestic sewage detecting device provided by the invention can realize the automatic detection of the domestic sewage of the ship, avoiding the problems of manual counting and counting, long time and large error, and the operation is simple and the precision is high; wherein the signal processing unit 13 and the display unit 14 can be integrated in a single-chip computer or a handheld device, so that the device is reduced in size and portable, and has important social and economic value in the maritime quantitative law enforcement process.

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Abstract

Disclosed is a ship domestic sewage detection device, which comprises: a micro flow control chip (11), which is provided with a micro runner of light transmission material and through which a sample solution containing bacteria with fluorescence dyeing flows; a fluorescence detecting unit (12) emitting laser to the micro runner and receiving the fluorescence generated by using the laser to excite the bacteria; a signal processing unit (13) for converting the fluorescence received by the fluorescence detecting unit (12) into an electrical signal and amplifying the electric signal; and a display unit (14) for counting and displaying the electric signal converted by the signal processing unit (13). The ship domestic sewage detection device may realize automatic detection of ship domestic sewage and has simple operation and high precision.

Description

一种船舶生活污水检测装置 技术领域  Ship domestic sewage detecting device
本发明属于船舶污水检测技术领域， 尤其涉及一种船舶生活污水检测装置。 背景技术  The invention belongs to the technical field of ship sewage detection, and particularly relates to a ship domestic sewage detecting device. Background technique
船舶生活污水是指来自于船上人员的日常生活排水， 其水体中含有的大量 致病菌给港口国的环境安全带来了严重威胁， 其引起的环境污染问题， 正逐步 受到世界各国的重视。  Ship domestic sewage refers to the daily life drainage of people from the ship. The large amount of pathogenic bacteria contained in the water body poses a serious threat to the environmental safety of the port state. The environmental pollution caused by it is gradually receiving the attention of all countries in the world.
因此， 国际海事组织在 1976年通过的 MEPC. 2 (VI )决议并于 2006年修订， 对污水中的大肠杆菌等进行了严格的规定， 例如， 大肠菌群几何平均值要求小 于 100个 /100ml等。  Therefore, the resolution of MEPC. 2 (VI) adopted by the International Maritime Organization in 1976 was revised in 2006 to strictly regulate E. coli in sewage. For example, the geometric mean value of coliforms is less than 100/100ml. Wait.
现有技术提供的船舶生活污水检测方式是将采集到的水体进行培养基培 养， 并在显微镜下观察， 通过人工进行目测计数， 其耗时长、 误差大， 无法在 实际中广泛应用。 发明内容  The domestic sewage detection method provided by the prior art is that the collected water body is cultured in a medium and observed under a microscope, and the manual measurement and counting are performed manually, which takes a long time and a large error, and cannot be widely applied in practice. Summary of the invention
本发明实施例的目的在于提供一种船舶生活污水检测装置， 以解决现有技 术提供的船舶生活污水检测方式是将采集到的水体进行培养基培养， 并在显微 镜下观察， 通过人工进行目测计数， 耗时长、 误差大的问题。  The object of the embodiments of the present invention is to provide a ship domestic sewage detecting device, which solves the problem that the domestic sewage detection method provided by the prior art is that the collected water body is cultured in a medium and observed under a microscope, and the visual counting is performed by manual. , a long time, a large error.
本发明实施例是这样实现的， 一种船舶生活污水检测装置， 所述装置包括: 供含有荧光染色细菌的样本溶液流过的微流控芯片， 所述微流控芯片包括 一透光材质形成的微流道；  The embodiment of the present invention is implemented as follows: a ship domestic sewage detecting device, the device comprising: a microfluidic chip for flowing a sample solution containing fluorescent staining bacteria, wherein the microfluidic chip comprises a light transmissive material Microchannel
向所述微流道发出激光， 并接收所述样本溶液中细菌被所述激光激发后产 生的荧光的荧光检测单元；  a fluorescence detecting unit that emits laser light to the microchannel and receives fluorescence of the sample solution in which the bacteria are excited by the laser;
将所述荧光检测单元接收到的荧光转换成电信号并对转换后的电信号进行 放大处理的信号处理单元；  a signal processing unit that converts fluorescence received by the fluorescence detecting unit into an electrical signal and amplifies the converted electrical signal;
连接所述信号处理单元、 对所述信号处理单元转换后的电信号进行计数并 显示的显示单元。 Connecting the signal processing unit, counting the electrical signals converted by the signal processing unit, and counting Display unit displayed.
上述船舶生活污水检测装置中， 所述微流道可以是由聚二甲基氧硅垸或聚 甲基丙烯酸甲酯制成的微流道。  In the above ship domestic sewage detecting device, the micro flow path may be a micro flow path made of polydimethyl siloxane or polymethyl methacrylate.
上述船舶生活污水检测装置中， 所述微流道沿入口-出口的方向进一步可以 包括入口流道、 收缩流道以及出口流道； 所述入口流道的内径和所述出口流道 的内径均大于所述收缩流道的内径。  In the above-mentioned ship domestic sewage detecting device, the micro flow channel may further include an inlet flow path, a contraction flow path, and an outlet flow path in the direction of the inlet-outlet; the inner diameter of the inlet flow path and the inner diameter of the outlet flow path are both It is larger than the inner diameter of the contraction flow path.
上述船舶生活污水检测装置中， 所述微流控芯片还可以包括：  In the above-mentioned ship domestic sewage detecting device, the microfluidic chip may further include:
置于所述出口流道端、 驱动所述样本溶液在所述微流道中流动的动力单元。 上述船舶生活污水检测装置中， 所述荧光检测单元可以包括：  A power unit placed at the outlet flow path end to drive the sample solution to flow in the micro flow channel. In the above-mentioned ship domestic sewage detecting device, the fluorescence detecting unit may include:
发出激光的激光器；  a laser that emits laser light;
第一滤光片， 用于滤除所述激光器发出的所述激光中的杂散光；  a first filter for filtering stray light in the laser emitted by the laser;
二向色镜， 用于反射经所述第一滤光片滤光后的激光；  a dichroic mirror for reflecting the laser light filtered by the first filter;
第一聚集透镜， 用于将所述二向色镜反射的激光聚集于所述收缩流道， 并 将所述收缩流道发出的所述荧光聚集于所述二向色镜；  a first collecting lens for collecting the laser light reflected by the dichroic mirror in the contracting flow channel, and collecting the fluorescent light emitted by the contracting flow channel to the dichroic mirror;
第二滤光片， 用于滤除经所述二向色镜透射的所述荧光中的激光干扰； 第二聚集透镜， 用于将所述第二滤光片滤光后的所述荧光聚集于所述信号 处理单元。  a second filter for filtering laser interference in the fluorescence transmitted through the dichroic mirror; a second collecting lens for collecting the fluorescent light after filtering the second filter In the signal processing unit.
上述船舶生活污水检测装置中， 所述信号处理单元可以包括：  In the ship domestic sewage detecting device, the signal processing unit may include:
转换模块， 用于将所述第二聚集透镜聚集的所述荧光转换为电信号； 信号放大模块， 用于将所述转换模块转换后的所述电信号进行放大处理； 计数模块， 用于对所述信号放大模块放大处理后的电信号进行计数后， 输 出给所述显示单元。  a conversion module, configured to convert the fluorescence collected by the second concentrating lens into an electrical signal; a signal amplification module, configured to perform amplification processing on the electrical signal converted by the conversion module; The signal amplification module amplifies the processed electrical signal and outputs it to the display unit.
应用本发明提供的船舶生活污水检测装置可以实现对船舶生活污水的自动 检测， 避免了现有通过人工进行目测计数， 耗时长、 误差大的问题， 且操作简 单、 精度高； 其中的信号处理单元和显示单元可集成于单片机或手持设备中， 以使得装置体积减小， 便于携带， 在海事量化执法过程中， 具有重要的社会意 义和经济价值。 附图说明  The ship domestic sewage detecting device provided by the invention can realize the automatic detection of the domestic sewage of the ship, avoiding the problems of manual counting and counting, long time and large error, and the operation is simple and the precision is high; wherein the signal processing unit The display unit and the display unit can be integrated into the single chip microcomputer or the handheld device, so that the device volume is reduced and portable, and has important social significance and economic value in the maritime quantitative law enforcement process. DRAWINGS
图 1是本发明提供的船舶生活污水检测装置的结构原理图; 图 2是图 1中微流控芯片以及荧光检测单元的结构图; 1 is a schematic structural view of a ship domestic sewage detecting device provided by the present invention; 2 is a structural view of the microfluidic chip and the fluorescence detecting unit of FIG. 1;
图 3是图 1中信号处理单元的结构图。  Figure 3 is a structural diagram of the signal processing unit of Figure 1.
具体实施方式 detailed description
为了使本发明的目的、 技术方案及优点更加清楚明白， 以下结合附图及实 施例， 对本发明进行进一步详细说明。 应当理解， 此处所描述的具体实施例仅 仅用以解释本发明， 并不用于限定本发明。  The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
图 1示出了本发明提供的船舶生活污水检测装置的结构原理。  Fig. 1 shows the structural principle of the ship domestic sewage detecting device provided by the present invention.
本发明提供的船舶生活污水检测装置包括： 供含有荧光染色细菌的样本溶 液流过的微流控芯片 11， 其包括一透光材质形成的微流道； 向微流道发出激光， 并接收样本溶液中细菌被该激光激发后产生的荧光的荧光检测单元 12; 将荧光 检测单元 12接收到的荧光转换成电信号并对转换后的电信号进行放大处理的信 号处理单元 13; 连接信号处理单元 13、 对信号处理单元 13转换后的电信号进 行计数并显示的显示单元 14。  The ship domestic sewage detecting device provided by the invention comprises: a microfluidic chip 11 for flowing a sample solution containing fluorescent dyeing bacteria, comprising a micro flow channel formed by a light transmissive material; emitting laser light to the micro flow channel, and receiving the sample a fluorescence detecting unit 12 that generates fluorescence after being excited by the laser in the solution; a signal processing unit 13 that converts fluorescence received by the fluorescence detecting unit 12 into an electrical signal and amplifies the converted electrical signal; and connects the signal processing unit 13. A display unit 14 that counts and displays the converted electrical signal by the signal processing unit 13.
应用本发明提供的船舶生活污水检测装置可以实现对船舶生活污水的自动 检测， 避免了现有通过人工进行目测计数， 耗时长、 误差大的问题， 且操作简 单、 精度高； 其中的信号处理单元 13和显示单元 14可集成于单片机或手持设 备中， 以使得装置体积减小， 便于携带， 在海事量化执法过程中， 具有重要的 社会意义和经济价值。  The ship domestic sewage detecting device provided by the invention can realize the automatic detection of the domestic sewage of the ship, avoiding the problems of manual counting and counting, long time and large error, and the operation is simple and the precision is high; wherein the signal processing unit 13 and the display unit 14 can be integrated in a single-chip computer or a handheld device, so that the device is reduced in size and portable, and has important social and economic value in the maritime quantitative law enforcement process.
其中的透光材质优选为聚二甲基氧硅垸 （PDMS ) 制成的流道或聚甲基丙烯 酸甲酯 （PMMA);  The light transmissive material is preferably a channel made of polydimethyl siloxane (PDMS) or polymethyl methacrylate (PMMA);
图 2示出了图 1中微流控芯片 11以及荧光检测单元 12的结构。  Fig. 2 shows the structure of the microfluidic chip 11 and the fluorescence detecting unit 12 of Fig. 1.
其中， 微流控芯片 11 的微流道沿入口 -出口的方向进一步包括： 入口流道 111、 收缩流道 112、 出口流道 114; 且入口流道 111的内径和出口流道 114的 内径均大于收缩流道 112的内径。微流控芯片 11还包括：置于出口流道 114端、 驱动样本溶液在微流道中流动的动力单元 113。  The micro flow channel of the microfluidic chip 11 further includes an inlet flow channel 111, a contraction flow channel 112, and an outlet flow channel 114 in the direction of the inlet-outlet port; and the inner diameter of the inlet flow channel 111 and the inner diameter of the outlet flow channel 114 are both It is larger than the inner diameter of the contraction flow passage 112. The microfluidic chip 11 further includes a power unit 113 placed at the end of the outlet flow path 114 to drive the sample solution to flow in the micro flow path.
其中， 荧光检测单元 12包括： 发出激光的激光器 126; 第一滤光片 125， 用于滤除激光器 126发出的激光中的杂散光； 二向色镜 122， 用于反射经第一滤 光片 125滤光后的激光； 第一聚集透镜 121， 用于将二向色镜 122反射的激光聚 集于收缩流道 112， 并将收缩流道 112发出的荧光聚集于二向色镜 122; 第二滤 光片 123， 用于滤除经二向色镜 122 透射的荧光中的激光干扰； 第二聚集透镜 124， 用于将第二滤光片 123滤光后的荧光聚集于信号处理单元 13。 The fluorescence detecting unit 12 includes: a laser 126 that emits laser light; a first filter 125 for filtering stray light in the laser light emitted by the laser 126; and a dichroic mirror 122 for reflecting the first filter 125 filtered laser light; a first collecting lens 121 for collecting the laser light reflected by the dichroic mirror 122 in the shrinking flow path 112, and collecting the fluorescent light emitted from the shrinking flow path 112 to the dichroic mirror 122; Filter The light sheet 123 is configured to filter out laser interference in the fluorescence transmitted through the dichroic mirror 122; and the second focusing lens 124 is configured to collect the fluorescence filtered by the second filter 123 in the signal processing unit 13.
图 3是图 1中信号处理单元 13的结构。  Figure 3 is a diagram showing the structure of the signal processing unit 13 of Figure 1.
信号处理单元 13包括： 转换模块 131， 用于将第二聚集透镜 124聚集的荧 光转换为电信号； 信号放大模块 132， 用于将转换模块 131转换后的电信号进行 放大处理； 计数模块 134， 用于对信号放大模块 132放大处理后的电信号进行计 数后， 输出给显示单元 14。  The signal processing unit 13 includes: a conversion module 131, configured to convert the fluorescence collected by the second focusing lens 124 into an electrical signal; a signal amplifying module 132, configured to perform amplification processing on the converted electrical signal of the conversion module 131; The electrical signals used for amplifying the signal amplification module 132 are counted and output to the display unit 14.
应用本发明提供的船舶生活污水检测装置可以实现对船舶生活污水的自动 检测， 避免了现有通过人工进行目测计数， 耗时长、 误差大的问题， 且操作简 单、 精度高； 其中的信号处理单元 13和显示单元 14可集成于单片机或手持设 备中， 以使得装置体积减小， 便于携带， 在海事量化执法过程中， 具有重要的 社会意义和经济价值。  The ship domestic sewage detecting device provided by the invention can realize the automatic detection of the domestic sewage of the ship, avoiding the problems of manual counting and counting, long time and large error, and the operation is simple and the precision is high; wherein the signal processing unit 13 and the display unit 14 can be integrated in a single-chip computer or a handheld device, so that the device is reduced in size and portable, and has important social and economic value in the maritime quantitative law enforcement process.
以上所述， 仅为本发明较佳的具体实施方式， 但本发明的保护范围并不局 限于此， 任何熟悉本技术领域的技术人员在本发明揭露的技术范围内， 根据本 发明的技术方案及其发明构思加以等同替换或改变， 都应涵盖在本发明的保护 范围之内。  The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, and any technical person skilled in the art within the technical scope disclosed by the present invention, the technical solution according to the present invention Equivalent substitutions or modifications of the inventive concept are intended to be included within the scope of the invention.

Claims

权 利 要 求 书 Claim
1、 一种船舶生活污水检测装置， 其特征在于， 所述装置包括： A ship domestic sewage detecting device, characterized in that the device comprises:
供含有荧光染色细菌的样本溶液流过的微流控芯片， 所述微流控芯片包括 一透光材质形成的微流道；  a microfluidic chip for flowing a sample solution containing fluorescently stained bacteria, the microfluidic chip comprising a microfluidic channel formed of a light transmissive material;
向所述微流道发出激光， 并接收所述样本溶液中细菌被所述激光激发后产 生荧光的荧光检测单元；  And emitting a laser to the microchannel, and receiving a fluorescence detecting unit that generates fluorescence after the bacteria in the sample solution are excited by the laser;
将所述荧光检测单元接收到的荧光转换成电信号并对转换后的电信号进行 放大处理的信号处理单元；  a signal processing unit that converts fluorescence received by the fluorescence detecting unit into an electrical signal and amplifies the converted electrical signal;
连接所述信号处理单元、 对所述信号处理单元转换后的电信号进行计数并 显示的显示单元。  And a display unit that connects the signal processing unit, counts and displays the converted electrical signal of the signal processing unit.
2、 如权利要求 1所述的船舶生活污水检测装置， 其特征在于， 所述微流 道是由聚二甲基氧硅垸或聚甲基丙烯酸甲酯制成的微流道。  The ship domestic sewage detecting device according to claim 1, wherein the microchannel is a microchannel made of polydimethylsiloxane or polymethyl methacrylate.
3、 如权利要求 1所述的船舶生活污水检测装置， 其特征在于， 所述微流 道沿入口-出口的方向进一步包括入口流道、 收缩流道以及出口流道； 所述入口 流道的内径和所述出口流道的内径均大于所述收缩流道的内径。  The ship domestic sewage detecting device according to claim 1, wherein the micro flow path further includes an inlet flow path, a contraction flow path, and an outlet flow path in an inlet-outlet direction; Both the inner diameter and the inner diameter of the outlet flow passage are larger than the inner diameter of the contraction flow passage.
4、 如权利要求 3所述的船舶生活污水检测装置， 其特征在于， 所述微流 控芯片还包括：  4. The ship domestic sewage detecting device according to claim 3, wherein the microfluidic chip further comprises:
置于所述出口流道端、 驱动所述样本溶液在所述微流道中流动的动力单元。 A power unit placed at the outlet flow path end to drive the sample solution to flow in the micro flow channel.
5、 如权利要求 3所述的船舶生活污水检测装置， 其特征在于， 所述荧光 诱导检测单元包括： The ship domestic sewage detecting device according to claim 3, wherein the fluorescence inducing detecting unit comprises:
发出激光的激光器；  a laser that emits laser light;
第一滤光片， 用于滤除所述激光器发出的所述激光中的杂散光；  a first filter for filtering stray light in the laser emitted by the laser;
二向色镜， 用于反射经所述第一滤光片滤光后的激光；  a dichroic mirror for reflecting the laser light filtered by the first filter;
第一聚集透镜， 用于将所述二向色镜反射的激光聚集于所述收缩流道， 并 将所述收缩流道发出的所述荧光聚集于所述二向色镜；  a first collecting lens for collecting the laser light reflected by the dichroic mirror in the contracting flow channel, and collecting the fluorescent light emitted by the contracting flow channel to the dichroic mirror;
第二滤光片， 用于滤除经所述二向色镜透射的所述荧光中的激光干扰； 第二聚集透镜， 用于将所述第二滤光片滤光后的所述荧光聚集于所述信号 处理单元。  a second filter for filtering laser interference in the fluorescence transmitted through the dichroic mirror; a second collecting lens for collecting the fluorescent light after filtering the second filter In the signal processing unit.
6、 如权利要求 5所述的船舶生活污水检测装置， 其特征在于， 所述信号 采集及处理单元包括： 转换模块， 用于将所述第二聚集透镜聚集的所述荧光转换为电信号； 信号放大模块， 用于将所述转换模块转换后的的所述电信号进行放大处理； 计数模块， 用于对所述信号放大模块放大处理后的电信号进行计数后， 输 出给所述显示单元。 The ship domestic sewage detecting device according to claim 5, wherein the signal collecting and processing unit comprises: a conversion module, configured to convert the fluorescence collected by the second concentrating lens into an electrical signal; a signal amplification module, configured to perform amplification processing on the electrical signal converted by the conversion module; The electrical signals amplified by the signal amplifying module are counted and output to the display unit.
PCT/CN2011/078008 2011-08-04 2011-08-04 Ship domestic sewage detection device WO2013016873A1 (en)

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