CN103352000A - Detecting and counting system for living microorganisms in ship's ballast water - Google Patents
Detecting and counting system for living microorganisms in ship's ballast water Download PDFInfo
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- CN103352000A CN103352000A CN201310293958XA CN201310293958A CN103352000A CN 103352000 A CN103352000 A CN 103352000A CN 201310293958X A CN201310293958X A CN 201310293958XA CN 201310293958 A CN201310293958 A CN 201310293958A CN 103352000 A CN103352000 A CN 103352000A
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Abstract
The invention discloses a detecting and counting system for living microorganisms in a ship's ballast water. The detecting and counting system comprises a microfluidic chip, a radio frequency detecting electrode, a microwave high-frequency connector, a PNA (personality number analysis) network analyzer, a display and a printer; the microfluidic chip is integrated with a micro-channel, the radio frequency detecting electrode and the microwave high-frequency connector; the radio frequency detecting electrode is arranged on a micro-channel detecting section; the output end of the PNA network analyzer is connected with the display and the printer through a signal optical cable respectively, and the PNA network analyzer is used for collecting, filtering and analyzing detected signals. According to the invention, based on the principle of a radio frequency conductivity method, the microfluidic chip is adopted for detecting, so that the size of a detecting device is small, the integration level of the detecting device is high, the detecting is fast, sample consumption is less, energy consumption is lower, and the integral cost is lower. In the invention, signal acquisition, analysis and the like are all accomplished by the PNA network analyzer, and the PNA network analyzer works steadily, has a simple integral component structure and is convenient to operate.
Description
Technical field
The present invention relates to a kind of detection and number system to the microorganism that survives in microorganism is counted and state is distinguished anyway the method, particularly a kind of ballast water for ship.
Background technology
For a long time, boats and ships guarantee stability and safety of navigation with water ballast always.According to the needs of Shipping Business, adjust the drinking water of boats and ships and the metacentric height that steadily reaches safety of hull vertical, horizontal, reduce hull strained, reduce ship vibration, and improve empty cabin seaworthiness.
In recent years, ballast water for ship carries environmental protection, the ecological problem that adventive and pathogenic agent cause and more and more causes the attention of various countries, and studies show that ballast water for ship is to cause the in recent decades one of the main reasons of biotic intrusion problem.Therefore, for better control and management ballast water for ship, reduce the risk of biotic intrusion, International Maritime Organizaton (IMO) has passed through " Management of Ships ' Ballast Water and settling management and control pact " in 2004, must be through processing before the pact mandatory provision ballast water for ship discharging, and satisfy the D-2 emission standard: minimum size be less than 10 more than or equal to the survival biology of 50 μ m in (1) every cubic metre; (2) in every milliliter minimum size less than 50 μ m but be less than 10 more than or equal to the survival biology of 10 μ m; (3) indicator microoraganism of discharging should not surpass the concentration of following regulation: 1. every 100mL is less than 1 cfu(colony-forming unit) poisonous vibrio cholerae (O1 and O139) or less than the 1g zooplankton sample (weight in wet base) of 1 cfu; 2. every 100mL is less than the intestinal bacteria of 250 cfu; 3. every 100mL is less than the enterococcus bacteria of 100 cfu.Wherein, Port State duly authorized official has the right according to Guidelines see and whether satisfy cleanup standard, but the sampling analysis time can not to be caused improperly delay to boats and ships the sampling of taking a sample of the ballast water for ship after processing.
Therefore, in order to satisfy pact for the emission request of ballast water for ship, just need to carry out to the handling property of ballast water for ship accordance and detect.Microorganism detection in the current ballast water for ship and method of counting mainly contain fluorescent microscope counting process, opticmicroscope counting process, cultivate the methods such as counting process, digital image analysis method, the anti-method of solution resistance, photomicrography method, flow cytometry, molecular detecting method and biochemical vigor detection.But these methods are all more time-consuming, and need to utilize relatively ripe experimental installation and instrument to analyze, and are bulky, some detecting instrument even technician's operation that need to be special, and some detection method can't be distinguished the life or death of microorganism.
Summary of the invention
Be to solve prior art above shortcomings parts, the present invention to propose a kind of simple in structure, detection speed fast, detection and the number system of microorganism cells in the ballast water for ship of working stability.
To achieve these goals, technical scheme of the present invention is as follows:
Detection and the number system of survival microorganism comprise micro-fluidic chip, radio frequency detecting electrode, microwave high-frequency junctor, PNA network analyzer, indicating meter, printer and housing in a kind of ballast water for ship; Described micro-fluidic chip is integrated with microchannel, radio frequency detecting electrode and microwave high-frequency junctor; The cross section of described microchannel is that the height of rectangle and internal passages remains unchanged, be divided into entrance, detection segment and outlet section, entrance and outlet section passage width equate, the detection segment passage width is 1/5 of entrance passage, the length of detection segment passage is 5 times of detection segment passage width, the width of radio frequency detecting electrode is 1/10 of detecting end passage length, the length of radio frequency detecting electrode is 10 times of its width, and radio frequency detecting electrode spacing is 1/2 of radio frequency detecting electrode width, entrance and outlet section channel transition adopt contraction transition, its transition length and detection segment equal in length to the passage of detection segment passage; Described radio frequency detecting electrode is arranged on the detection segment of microchannel; Described microwave high-frequency junctor one end links to each other with the radio frequency detecting electrode, and the other end links to each other with the PNA network analyzer; Described micro-fluidic chip is installed in the housing; Described PNA network analyzer, indicating meter and printer integrated installation are in housing; The output terminal of described PNA network analyzer links to each other with indicating meter and printer respectively by the signal optical cable, and the PNA network analyzer gathers, filters and analyze the signal that detects.
Compared with prior art, the present invention has following beneficial effect:
1, the present invention is based on radio frequency electrical inducing defecation by enema and suppository principle, adopt micro-fluidic chip to detect, the detection means small volume, the detection part integrated level is high, and detects fast, and sample consumption is few, and energy consumption is lower, and holistic cost is lower.
2, sampling and analysis of signal of the present invention etc. is finished by the PNA network analyzer, PNA network analyzer working stability, and integral unit is simple in structure, easy to operate.
3, circuit card integrated level of the present invention is high, and compact construction is convenient for carrying, and makes things convenient for Site Detection, especially is convenient to the fields such as maritime affair law enforcement, environmental monitoring.
Description of drawings
The present invention has accompanying drawing 1 width of cloth, wherein:
Fig. 1 is system schematic of the present invention.
Among the figure: 1, liquid storage tank, 2, entrance, 3, the radio frequency detecting electrode, 4, detection segment, 5, outlet section, 6, waste liquid pool, 7, microorganism cells, 8, the microwave high-frequency junctor, 9, the PNA network analyzer, 10, indicating meter, 11, printer, 12, housing.
Embodiment
Below in conjunction with accompanying drawing the present invention is further described.As scheme shown in the l, the detection system of microorganism cells in a kind of ballast water for ship comprises micro-fluidic chip, radio frequency detecting electrode 3, microwave high-frequency junctor 8, PNA network analyzer 9, indicating meter 10, printer 11 and housing 12; Described micro-fluidic chip is integrated with microchannel, radio frequency detecting electrode 3 and microwave high-frequency junctor 8; The cross section of microchannel is that rectangle and internal passages height are fixed, be divided into entrance 2, detection segment 4 and outlet section 5, entrance 2 and outlet section 5 passage widths equate, detection segment 4 passage widths are 1/5 of entrance 2 passages, the length of detection segment 4 passages is 5 times of detection segment passage width, the width of radio frequency detecting electrode 3 is 1/10 of detection segment 4 passage lengths, the length of electrode is 10 times of its width, and interelectrode distance is 1/2 of its width, entrance 2 and outlet section 5 channel transition adopt contraction transition, its transition length and detection segment equal in length to the passage of detection segment 4 passages; The fluid of required detection is entered by fluid entrance 1; Described radio frequency detecting electrode 3 is arranged on the microchannel detection segment 4; Described microwave high-frequency junctor 8 one ends link to each other with radio frequency detecting electrode 3, and the other end links to each other with PNA network analyzer 9; Described micro-fluidic chip is installed in the housing 12; Described PNA network analyzer 9, indicating meter 10 and printer 11 integrated installations are in housing 12; The output terminal of described PNA network analyzer 9 links to each other with indicating meter 10 and printer 11 respectively by the signal optical cable, and 9 pairs of signals that detect of PNA network analyzer gather, filter and analyze.
Principle of work of the present invention is as follows: the present invention adopts the radio frequency conductance detection, because the present invention uses the high-frequency radio frequency signal, radio frequency detecting electrode 3 links to each other with PNA network analyzer 9, when a microorganism is arranged by radio frequency detecting electrode 3 place, on PNA network analyzer 9, can demonstrate the pulse signal of certain amplitude, the number of the pulse signal that record forms, thus realize the microorganism cells that flows through is counted; Although dead, live microorganism of the same race measure-alike but content different, therefore, on PNA network analysis 9, can demonstrate the pulse signal of different amplitudes, and corresponding frequency of each signal, 9 pairs of signals of PNA network analyzer gather, filter and analyze, according to the different wave that occurs in the PNA network analyzer 9, distinguish the life or death of microorganism.
Working process of the present invention is as follows: from liquid storage tank 1 a certain amount of solution that contains microorganism cells 7 is injected into the microchannel, the liquid that contains microorganism cells 7 the flow through entrance 4 of microchannel, detection segment 5, radio frequency detecting electrode 3, outlet section 6 finally enter waste liquid pool 6.When microorganism cells 7 passes through radio frequency detecting electrode 3 place, on PNA network analysis 9, can demonstrate the pulse signal of certain amplitude, the number of the pulse signal that record forms, thus realize the microorganism cells that flows through is counted; Although dead, live microorganism of the same race measure-alike but content different, therefore, on PNA network analysis 9, can demonstrate the pulse signal of different amplitudes, and corresponding frequency of each signal, 9 pairs of signals of PNA network analyzer gather, filter and analyze, according to the different wave that occurs in the PNA network analyzer 9, distinguish the life or death state of same microorganism cells.If want to observe sometime in the section, the signal waveform that microorganism cells 7 produces during by radio frequency detecting electrode 3 place can use printer 11 that it is printed.
Claims (1)
1. detection and the number system of survival microorganism in the ballast water for ship is characterized in that: comprise micro-fluidic chip, radio frequency detecting electrode (3), microwave high-frequency junctor (8), PNA network analyzer (9), indicating meter (10), printer (11) and housing (12); Described micro-fluidic chip is integrated with microchannel, radio frequency detecting electrode (3) and microwave high-frequency junctor (8); The cross section of described microchannel is that the height of rectangle and internal passages remains unchanged, be divided into entrance (2), detection segment (4) and outlet section (5), entrance (2) and outlet section (5) passage width equate, detection segment (4) passage width is 1/5 of entrance (2) passage, the length of detection segment (4) passage is 5 times of detection segment (4) passage width, the width of radio frequency detecting electrode (3) is 1/10 of detecting end passage length, the length of radio frequency detecting electrode (3) is 10 times of its width, and radio frequency detecting electrode (3) spacing is 1/2 of radio frequency detecting electrode (3) width, entrance (2) and outlet section (5) channel transition adopt contraction transition, its transition length and detection segment (4) equal in length to the passage of detection segment (4) passage; Described radio frequency detecting electrode (3) is arranged on the microchannel detection segment (4); Described microwave high-frequency junctor (8) one ends link to each other with radio frequency detecting electrode (3), and the other end links to each other with PNA network analyzer (9); Described micro-fluidic chip is installed in the housing (12); Described PNA network analyzer (9), indicating meter (10) and printer (11) integrated installation are in housing (12); The output terminal of described PNA network analyzer (9) links to each other with indicating meter (10) and printer (11) respectively by the signal optical cable, and PNA network analyzer (9) gathers, filters and analyze the signal that detects.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673665A (en) * | 2015-03-05 | 2015-06-03 | 韩先锋 | Colony count alarming device for vessel ballast water |
| CN106442920A (en) * | 2016-11-11 | 2017-02-22 | 大连海事大学 | Ship ballast water drainage real-time monitoring device and method |
| CN107543907A (en) * | 2017-09-08 | 2018-01-05 | 大连海事大学 | A kind of monitoring in real time and the device of detection ballast water for ship water quality |
| CN110118771A (en) * | 2019-03-29 | 2019-08-13 | 亳州学院 | A kind of intelligent microscopic examination device and microscopy method for active sludge microorganism |
| CN111272757A (en) * | 2020-03-12 | 2020-06-12 | 大连海事大学 | Device and method for judging microalgae activity in ship ballast water based on electric movement speed of microalgae |
| CN114870917A (en) * | 2022-05-09 | 2022-08-09 | 南京大学 | Microfluidic chip for identifying different cells and preparation method and detection platform thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202002870U (en) * | 2010-12-01 | 2011-10-05 | 张洪朋 | Oil liquid particle distinguished counting device |
| KR101071116B1 (en) * | 2007-11-14 | 2011-10-07 | 대한민국 (식품의약품안전청장) | A detector of humoral microorganism |
| CN102628826A (en) * | 2012-04-20 | 2012-08-08 | 大连海事大学 | Oil concentration rapid detection apparatus for oily sewage |
| CN103105352A (en) * | 2013-01-28 | 2013-05-15 | 大连海事大学 | Device and method for rapidly detecting surviving unicellular organisms in ship ballast water |
-
2013
- 2013-07-12 CN CN201310293958XA patent/CN103352000A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101071116B1 (en) * | 2007-11-14 | 2011-10-07 | 대한민국 (식품의약품안전청장) | A detector of humoral microorganism |
| CN202002870U (en) * | 2010-12-01 | 2011-10-05 | 张洪朋 | Oil liquid particle distinguished counting device |
| CN102628826A (en) * | 2012-04-20 | 2012-08-08 | 大连海事大学 | Oil concentration rapid detection apparatus for oily sewage |
| CN103105352A (en) * | 2013-01-28 | 2013-05-15 | 大连海事大学 | Device and method for rapidly detecting surviving unicellular organisms in ship ballast water |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673665A (en) * | 2015-03-05 | 2015-06-03 | 韩先锋 | Colony count alarming device for vessel ballast water |
| CN104673665B (en) * | 2015-03-05 | 2016-06-22 | 韩先锋 | The clump count warning devices of ballast water for ship |
| CN106442920A (en) * | 2016-11-11 | 2017-02-22 | 大连海事大学 | Ship ballast water drainage real-time monitoring device and method |
| CN107543907A (en) * | 2017-09-08 | 2018-01-05 | 大连海事大学 | A kind of monitoring in real time and the device of detection ballast water for ship water quality |
| CN110118771A (en) * | 2019-03-29 | 2019-08-13 | 亳州学院 | A kind of intelligent microscopic examination device and microscopy method for active sludge microorganism |
| CN111272757A (en) * | 2020-03-12 | 2020-06-12 | 大连海事大学 | Device and method for judging microalgae activity in ship ballast water based on electric movement speed of microalgae |
| CN111272757B (en) * | 2020-03-12 | 2022-11-25 | 大连海事大学 | Device and method for judging microalgae activity in ship ballast water based on electric movement speed of microalgae |
| CN114870917A (en) * | 2022-05-09 | 2022-08-09 | 南京大学 | Microfluidic chip for identifying different cells and preparation method and detection platform thereof |
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Application publication date: 20131016 |