CN102250807B - Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof - Google Patents
Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a microbial agent for Chinese silvergrass ensilage as well as a preparation method and application thereof. The microbial agent is composed of 0.3*10<9>-1.5*10<9> cfu/g Lactobacillus casei, 0.3*10<9>-1.5*10<9> cfu/g of Lactobacillus plantarum, 0.4*10<9>-2*10<9> cfu/g of Saccharomyces cerevisiae and 0.4*10<9>-2*10<9> cfu/g of Trichoderma viride, wherein the total amount of microorganisms is 0.68*10<9>-3.4*10<9> cfu/g. By using the microbial agent to manufacture the Chinese silvergrass ensilage, the maturation time of the Chinese silvergrass ensilage is shortened, the probability of feed deterioration is reduced, the protein content is increased, the cellulose content is reduced, and the palatability is enhanced.
Description
Technical field
The present invention relates to a kind of microbiobacterial agent and its preparation method and application, particularly a kind of microbiobacterial agent for Chinese silvergrass class silage and its preparation method and application.
Background technology
Ensiling refers to and under air-proof condition, makes silage fermentation can within considerable time, keep the metastable a kind of preservation technique of its quality.Silage refer to the green grass that easily damaged by the oxygen consumption microorganism or the high storage of other moisture content under anaerobic condition by lactobacillus-fermented make can prolonged preservation feed.This Feed Energy is effectively preserved the nutritive ingredient of dark green plant, guarantees fresh and tender juice, quality softness, good palatability when raw material is dark green, food consumption is large, digestive utilization ratio is high, is a kind of extraordinary feed to ruminating animals such as cattle and sheep especially.Nutrition rate of loss 8% ~ 10% after the ensiling ensiling, reach 30%～50% even higher and dry preservation nutritive loss rate.In ensilage, due to the effect of microorganism, the feed fibre cellulose content can descend 3%～6%, and crude protein content improves 0.8%～2%.The tradition ensiling due to utilize be green fodder self with microorganism fermented, thereby quality is difficult to control, and the situation of ensiling failure usually occurs, thereby causes that the feed palatability is poor, quality descends.The Guangxi province mountain and hill is numerous, and wild Chinese silvergrass resource is very abundant, how effectively to utilize these resources to have a great deal of practical meanings for the raising of ruminant animal.
In current disclosed patent documentation, China Patent No. is that ZL200610078589.2 " a kind of composite microbial feed additive and production method thereof and purposes " discloses a kind of microorganism feed addictive, comprises yeast saccharomyces cerevisiae, plant lactobacillus and lactobacterium casei cheese subspecies.This microorganism is to be added in animal-feed, is mainly in order to improve efficiency of feed utilization and cultivated animals body immunity, reduces ill probability and the quality of improving cultivated animals, reduces the animal rearing cost.All not identical with effect and the purposes of the microbiobacterial agent for Chinese silvergrass class silage of patent application of the present invention.
Summary of the invention
The technical problem to be solved in the present invention is: a kind of microbiobacterial agent for Chinese silvergrass class silage and its preparation method and application is provided, adopt the making of this microbiobacterial agent for Chinese silvergrass class silage, shortened Chinese silvergrass silage maturation time, increased protein content, reduce content of cellulose, overcome above-mentioned the deficiencies in the prior art part.
The technical scheme solved the problems of the technologies described above is: a kind of microbiobacterial agent for Chinese silvergrass class silage, this microbiobacterial agent be the Cheesecake Bacterium lacticum (
lactobacillus casei) 0.3 * 10
9~ 1.5 * 10
9cfu/g, plant lactobacillus (
lactobacillus plantarum) 0.3 * 10
9~ 1.5 * 10
9cfu/g, yeast saccharomyces cerevisiae (
saccharomyces cerevisiae) 0.4 * 10
8~ 0.2 * 10
9cfu/g and viride (
trichoderma viride) 0.4 * 10
8~ 0.2 * 10
9cfu/g forms, and the microorganism total amount is 0.68 * 10
9～3.4 * 10
9cfu/g.
This microbiobacterial agent is Cheesecake Bacterium lacticum 1.0 * 10
9~ 1.2 * 10
9cfu/g, plant lactobacillus 1.0 * 10
9~ 1.2 * 10
9cfu/g, yeast saccharomyces cerevisiae 0.1 * 10
9~ 0.2 * 10
9cfu/g and viride 0.1 * 10
9~ 0.2 * 10
9cfu/g forms, and the microorganism total amount is 2.2 * 10
9～2.8 * 10
9cfu/g.
A kind of preparation method of the microbiobacterial agent for Chinese silvergrass class silage comprises the following steps:
(1) microorganism culturing:
A. the cultivation of lactobacterium casei:
Al. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4cl and KH
2pO
4concentration be 1.0g/L, make substratum after high-temperature sterilization;
A2. enlarged culturing step by step: the amount of yeast culture thing as required, adopt enlarged culturing step by step, get the original lactobacterium casei liquid spawn access of 5～10 μ l 100ml substratum, cultivate 10h～14h under 33～35 ℃, the condition of 140～160rpm, the lactobacterium casei bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml; If need more substantial yeast culture thing, be by volume more 5～10% inoculum size will before cultured lactobacterium casei kind receive in new substratum, cultivate 10～14h and get final product under 33～35 ℃, the condition of 140～160rpm, the lactobacterium casei bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml;
B. the cultivation of plant lactobacillus:
Bl. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4cl and KH
2pO
4concentration be 1.0g/L, make substratum after high-temperature sterilization;
B2. enlarged culturing step by step: the amount of yeast culture thing as required, adopt enlarged culturing step by step, get primordial plant Bacterium lacticum liquid spawn 5～10 μ l access 100ml substratum, cultivate 10～14h under 35～37 ℃, the condition of 140～160rpm, the plant lactobacillus bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml; If need more substantial yeast culture thing, be by volume more 5～10% inoculum size will before cultured plant lactobacillus kind receive in new substratum, cultivate 10～14h and get final product under 35～37 ℃, the condition of 140～160rpm, the plant lactobacillus bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml;
C. the cultivation of yeast saccharomyces cerevisiae:
Cl. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4the concentration of Cl is 2.0g/L, KH
2pO
4concentration be 1.5g/L, make substratum after high-temperature sterilization;
C2. enlarged culturing step by step: the amount of yeast culture thing as required, adopt enlarged culturing step by step, get the original yeast saccharomyces cerevisiae liquid spawn access of 5～10 μ l 100ml substratum, cultivate 12h～16h under 28～30 ℃, the condition of 140～160rpm, the yeast saccharomyces cerevisiae bacterial concentration after cultivation is 0.2 * 10
9~ 1.0 * 10
9cfu/ml; If need more substantial yeast culture thing, be by volume more 5～10% inoculum size will before cultured yeast saccharomyces cerevisiae bacterial classification receive in new substratum, cultivate 12～16h and get final product under 28～30 ℃, the condition of 140～160rpm, the yeast saccharomyces cerevisiae bacterial concentration after cultivation is 0.2 * 10
9~ 1.0 * 10
9cfu/ml;
D. the cultivation of viride:
D1. the preparation of substratum: remove the skin potato, add water boil 25～35min after being cut into small pieces, with after filtered through gauze, get filtered liquid, add 0.05～0.2g glucose and 0.08～0.1g agarose meter according to every gram potato, add glucose and agarose in filtered liquid, moisturizing is by the filtered liquid constant volume again, filtered liquid volume after constant volume and potato mass ratio are 5～6ml:1g, after high-temperature sterilization, be down flat plate and make solid medium, getting the original viride point of slant preservation plants on solid medium, 25～30 ℃, standing cultivation 5 days～7 days, treat that the mycelia surface grows a large amount of spores and gets final product, each flat board is got 1～3ml stroke-physiological saline solution and is rinsed the spore make the mycelia surface and come off and obtain spore suspension, by stroke-physiological saline solution, by the spore suspension concentration dilution, be 0.2 * 10 again
9~ 1.0 * 10
9cfu/ml is standby,
(2) thalline and the preparation of spore mixed solution:
By cultured lactobacterium casei bacterium liquid, plant lactobacillus bacterium liquid, yeast saccharomyces cerevisiae suspension and viride spore suspension, the ratio of 3:3:2:2 mixes by volume, remove the upper strata substratum after centrifugal, the thalline obtained uses the physiological saline of 10 ~ 15% centrifugal front bacterium liquid cumulative volumes to mix again;
(3) adsorption dry:
Get the bacterium liquid that step (2) obtains, by 1ml bacterium liquid: 10g wheat bran is got wheat bran, and bacterium liquid evenly is sprayed to the wheat bran surface, and fully mixes, and air-dryly below 40 ℃ is less than 15% to moisture, obtains microbial inoculum.
In step (1) microorganism culturing, described high-temperature sterilization refers at 115～121 ℃ of lower sterilizing 20～30min.
In step (1) microorganism culturing, described original lactobacterium casei is to separate from sour milk product; The primordial plant Bacterium lacticum is to separate from the Chinese silvergrass class silage of spontaneous fermentation; Original yeast saccharomyces cerevisiae is to separate from distillery's vinasse; Original viride is to separate from natural rotten wood.
Microbiobacterial agent of the present invention can be used in Chinese silvergrass class silage, its concrete using method is: with this microbiobacterial agent of the warm water soaking of 30～35 ℃ 25～40min, the bacterium liquid obtained is, after 1:95～1:105 mixes, to carry out the ensiling preservation by general Silaging method to get final product with the Chinese silvergrass class green fodder after pulverizing by weight; The weight ratio of described warm water and microbiobacterial agent is 450～500:1.
Adopt the making of the present invention's microbiobacterial agent for Chinese silvergrass class silage, the ensiling maturation time shortens 4 ~ 8 days than spontaneous fermentation; After ensiling 5 months, content of cellulose reduces by 3%～5% than the silage of spontaneous fermentation, and crude protein content increases by 5%～10% than the silage of spontaneous fermentation.Adopt the making of the present invention's microbiobacterial agent for Chinese silvergrass class silage, can shorten Chinese silvergrass silage maturation time, reduce the rotten probability of feed, increase protein content, reduce content of cellulose, strengthen palatability.
In addition, molasses are in industrial sugaring process, after crystallization of sucrose, remaining uncrystallizable, but still contain the liquid residue than polysaccharide, and it is the by product of sugar industry.A lot of sugar refineries are processed molasses as waste material.And the present invention adopts molasses to carry out the cultivation of microorganism, realized recycling of waste material, not only the preparation cost of microbial inoculum is low, and is the processing problem that sugar refinery has solved the waste material molasses to a certain extent, is conducive to environment protection.
Below, the technical characterictic of microbiobacterial agent for Chinese silvergrass class silage of the present invention and its preparation method and application is further described in conjunction with the embodiments.
Embodiment
Embodiment 1: for the microbiobacterial agent of Chinese silvergrass class silage, following bacterial classification, consist of:
Lactobacterium casei 1.5 * 10
9cfu/g
Plant lactobacillus 1.5 * 10
9cfu/g
Yeast saccharomyces cerevisiae 0.2 * 10
9cfu/g
Viride 0.2 * 10
9cfu/g
The microorganism total amount is 3.4 * 10
9cfu/g.
Embodiment 2: for the microbiobacterial agent of Chinese silvergrass class silage, following bacterial classification, consist of:
Lactobacterium casei 0.3 * 10
9cfu/g
Plant lactobacillus 0.3 * 10
9cfu/g
Yeast saccharomyces cerevisiae 0.4 * 10
8cfu/g
Viride 0.4 * 10
8cfu/g
The microorganism total amount is 0.68 * 10
9cfu/g.
Embodiment 3: for the microbiobacterial agent of Chinese silvergrass class silage, following bacterial classification, consist of:
Lactobacterium casei 1.0 * 10
9cfu/g
Plant lactobacillus 0.4 * 10
9cfu/g
Yeast saccharomyces cerevisiae 0.1 * 10
9cfu/g
Viride 0.2 * 10
9cfu/g
The microorganism total amount is 1.7 * 10
9cfu/g.
Embodiment 4: for the microbiobacterial agent of Chinese silvergrass class silage, following bacterial classification, consist of:
Lactobacterium casei 0.8 * 10
9cfu/g
Plant lactobacillus 1.0 * 10
9cfu/g
Yeast saccharomyces cerevisiae 0.15 * 10
9cfu/g
Viride 0.8 * 10
8cfu/g
The microorganism total amount is 2.03 * 10
9cfu/g.
Embodiment 5: the preparation method for the microbiobacterial agent of Chinese silvergrass class silage comprises the following steps:
(1) microorganism culturing:
A. the cultivation of lactobacterium casei:
Al. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4the concentration of Cl is 1.0g/L, KH
2pO
4concentration be 1.0g/L, 121 ℃ of sterilizing 20min make substratum;
A2. enlarged culturing step by step: get the original lactobacterium casei liquid spawn access of 10 μ l 100ml substratum, 33 ℃, 150rpm are cultivated 10h～14h, and the lactobacterium casei bacterial concentration obtained after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml; Cultured bacterium liquid 30mL is transferred in the new substratum of 300ml, and 33 ℃, 150rpm are cultivated 10h～14h, and the lactobacterium casei bacterial concentration obtained after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml.
B. the cultivation of plant lactobacillus:
Bl. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4the concentration of Cl is 1.0g/L, KH
2pO
4concentration be 1.0g/L, 121 ℃ of sterilizing 20min make substratum;
B2. enlarged culturing step by step: get 10 μ l primordial plant Bacterium lacticum liquid spawns access 100ml substratum, 37 ℃, 150rpm are cultivated 10h～14h, and the plant lactobacillus bacterial concentration obtained after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml; Cultured bacterium liquid 30mL is transferred in the new substratum of 300ml, and 37 ℃, 150rpm are cultivated 10h～14h, and the plant lactobacillus bacterial concentration obtained after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml.
C. the cultivation of yeast saccharomyces cerevisiae:
Cl. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4the concentration of Cl is 2.0g/L, KH
2pO
4concentration be 1.5g/L, 121 ℃ of sterilizing 20min make substratum;
C2. enlarged culturing step by step: get the original yeast saccharomyces cerevisiae liquid spawn access of 10 μ l 100ml substratum, 28 ℃, 150rpm are cultivated 12h～16h, and the yeast saccharomyces cerevisiae bacterial concentration after cultivation is 0.2 * 10
9~ 1.0 * 10
9cfu/ml; Cultured bacterium liquid 20ml is transferred in the new substratum of 200ml, and 28 ℃, 150rpm are cultivated 12h～16h, and the yeast saccharomyces cerevisiae bacterial concentration after cultivation is 0.2 * 10
9~ 1.0 * 10
9cfu/ml.
D. the cultivation of viride:
D1. the preparation of substratum: adopt the potato glucose culture medium culturing, get 200g peeling potato, be cut into small pieces, add in pot and add water boil 30min, with after 2 layers of filtered through gauze, get filtered liquid and add 20g glucose, the 20g agarose, supply water by the filtered liquid constant volume to 1000ml, after 121 ℃ of sterilizing 20min, be down flat plate and make solid medium.Getting the original viride bacterial classification point of slant preservation plants in solid medium, 28 ℃, standing cultivation 5 days～7 days, after the mycelia surface grows a large amount of spores, each flat board is got the spore that 2 ml stroke-physiological saline solution are washed lower mycelia surface, then is diluted to 0.2 * 10 by stroke-physiological saline solution
9~ 1.0 * 10
9cfu/ml makes spore suspension, prepares altogether spore suspension 200ml.
thalline and the preparation of spore mixed solution:
Get cultured lactobacterium casei bacterium liquid, each 300ml of plant lactobacillus bacterium liquid, the yeast saccharomyces cerevisiae suspension prepared, each 200ml of viride spore suspension, mix, and removes the upper strata substratum after centrifugal.Thalline is mixed evenly with 100ml physiological saline.
adsorption dry:
Get the thalline and the spore mixed solution 100ml that prepare, then get 1000g wheat bran, bacterium liquid evenly is sprayed to the wheat bran surface, and fully mix, air-dryly below 40 ℃ be less than 15% to moisture and obtain microbial inoculum.The contained microbe population of the microbial inoculum of making is: lactobacterium casei 0.3 * 10
9~ 1.5 * 10
9cfu/g, plant lactobacillus 0.3 * 10
9~ 1.5 * 10
9cfu/g, yeast saccharomyces cerevisiae 0.4 * 10
8~ 0.2 * 10
9cfu/g and viride 0.4 * 10
8~ 0.2 * 10
9cfu/g, the microorganism total amount is 0.68 * 10
9～3.4 * 10
9cfu/g.
In the present embodiment step (1) microorganism culturing, described original lactobacterium casei is to separate from sour milk product; The primordial plant Bacterium lacticum is to separate from the Chinese silvergrass class silage of spontaneous fermentation; Original yeast saccharomyces cerevisiae is to separate from distillery's vinasse; Original viride is to separate from natural rotten wood.
The cultivation of the lactobacterium casei of the present embodiment step (1) microorganism, plant lactobacillus, yeast saccharomyces cerevisiae is to adopt the method for enlarged culturing step by step, what specifically need through enlarged culturing, the amount of yeast culture thing is according to actual needs determined, can cultivate and obtain by one-level if the biomass needed is less, if need more substantial yeast culture thing, need to obtain by two-stage, three grades or more multistage cultivation; The cultivation of the viride of described step (1) microorganism is to adopt solid medium to be cultivated, and the quantity of bacterial classification is as required determined the quantity of the flat-plate solid substratum that needs preparation, with cultivation, obtains suitable bacterial classification amount.
Embodiment 6: the application for the microbiobacterial agent of Chinese silvergrass class silage at Chinese silvergrass class silage, soak 10g microbial inoculum 30min with the warm water 5kg of 30 ℃, then with after Chinese silvergrass class green fodder after 500kg pulverizes mixes, carry out the ensiling preservation by general Silaging method and get final product.
Above embodiment further illustrates of the present invention, can not be for the restriction to the invention protection domain.
Claims (4)
1. the microbiobacterial agent for Chinese silvergrass class silage is characterized in that: should comprise the following steps for preparation method of the microbiobacterial agent of Chinese silvergrass class silage:
(1) microorganism culturing:
A. the cultivation of lactobacterium casei:
Al. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4cl and KH
2pO
4concentration be 1.0g/L, make substratum after high-temperature sterilization;
A2. enlarged culturing step by step: the amount of yeast culture thing as required, adopt enlarged culturing step by step, get the original lactobacterium casei liquid spawn access of 5～10 μ l 100ml substratum, cultivate 10h～14h under 33～35 ℃, the condition of 140～160rpm, the lactobacterium casei bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml; If need more substantial yeast culture thing, be by volume more 5～10% inoculum size will before cultured lactobacterium casei kind receive in new substratum, cultivate 10～14h and get final product under 33～35 ℃, the condition of 140～160rpm, the lactobacterium casei bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml;
B. the cultivation of plant lactobacillus:
Bl. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4cl and KH
2pO
4concentration be 1.0g/L, make substratum after high-temperature sterilization;
B2. enlarged culturing step by step: the amount of yeast culture thing as required, adopt enlarged culturing step by step, get primordial plant Bacterium lacticum liquid spawn 5～10 μ l access 100ml substratum, cultivate 10～14h under 35～37 ℃, the condition of 140～160rpm, the plant lactobacillus bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml; If need more substantial yeast culture thing, be by volume more 5～10% inoculum size will before cultured plant lactobacillus kind receive in new substratum, cultivate 10～14h and get final product under 35～37 ℃, the condition of 140～160rpm, the plant lactobacillus bacterial concentration after cultivation is 1.0 * 10
9~ 5.0 * 10
9cfu/ml;
C. the cultivation of yeast saccharomyces cerevisiae:
Cl. the preparation of substratum: water dilutes 8～10 times by molasses in mass ratio, then adds NH
4cl and KH
2pO
4, make NH in molasses solution
4the concentration of Cl is 2.0g/L, KH
2pO
4concentration be 1.5g/L, make substratum after high-temperature sterilization;
C2. enlarged culturing step by step: the amount of yeast culture thing as required, adopt enlarged culturing step by step, get the original yeast saccharomyces cerevisiae liquid spawn access of 5～10 μ l 100ml substratum, cultivate 12h～16h under 28～30 ℃, the condition of 140～160rpm, the yeast saccharomyces cerevisiae bacterial concentration after cultivation is 0.2 * 10
9~ 1.0 * 10
9cfu/ml; If need more substantial yeast culture thing, be by volume more 5～10% inoculum size will before cultured yeast saccharomyces cerevisiae bacterial classification receive in new substratum, cultivate 12～16h and get final product under 28～30 ℃, the condition of 140～160rpm, the yeast saccharomyces cerevisiae bacterial concentration after cultivation is 0.2 * 10
9~ 1.0 * 10
9cfu/ml;
D. the cultivation of viride:
D1. the preparation of substratum: remove the skin potato, add water boil 25～35min after being cut into small pieces, with after filtered through gauze, get filtered liquid, add 0.05～0.2g glucose and 0.08～0.1g agarose meter according to every gram potato, add glucose and agarose in filtered liquid, moisturizing is by the filtered liquid constant volume again, filtered liquid volume after constant volume and potato mass ratio are 5～6ml:1g, after high-temperature sterilization, be down flat plate and make solid medium, getting the original viride point of slant preservation plants in solid medium, 25～30 ℃, standing cultivation 5 days～7 days, treat that the mycelia surface grows a large amount of spores and gets final product, each flat board is got 1～3ml stroke-physiological saline solution and is rinsed the spore make the mycelia surface and come off and obtain spore suspension, by stroke-physiological saline solution, by the spore suspension concentration dilution, be 0.2 * 10 again
9~ 1.0 * 10
9cfu/ml is standby,
(2) thalline and the preparation of spore mixed solution:
By cultured lactobacterium casei bacterium liquid, plant lactobacillus bacterium liquid, yeast saccharomyces cerevisiae suspension and viride spore suspension, the ratio of 3:3:2:2 mixes by volume, remove the upper strata substratum after centrifugal, the thalline obtained uses the physiological saline of 10 ~ 15% centrifugal front bacterium liquid cumulative volumes to mix again;
(3) adsorption dry:
Get the bacterium liquid that step (2) obtains, by 1ml bacterium liquid: 10g wheat bran is got wheat bran, and bacterium liquid evenly is sprayed to the wheat bran surface, and fully mixes, and air-dryly below 40 ℃ is less than 15% to moisture, obtains microbial inoculum.
2. the microbiobacterial agent for Chinese silvergrass class silage according to claim 1, it is characterized in that: in step (1) microorganism culturing, described high-temperature sterilization refers at 115～121 ℃ of lower sterilizing 20～30min.
3. the microbiobacterial agent for Chinese silvergrass class silage according to claim 1 and 2, it is characterized in that: in step (1) microorganism culturing, described original lactobacterium casei is to separate from sour milk product; The primordial plant Bacterium lacticum is to separate from the Chinese silvergrass class silage of spontaneous fermentation; Original yeast saccharomyces cerevisiae is to separate from distillery's vinasse; Original viride is to separate from natural rotten wood.
4. the application of the microbiobacterial agent for Chinese silvergrass class silage as claimed in claim 1, it is characterized in that: with this microbiobacterial agent of the warm water soaking of 30～35 ℃ 25～40min, the bacterium liquid obtained is, after 1:95～1:105 mixes, to carry out the ensiling preservation by general Silaging method to get final product with the Chinese silvergrass class green fodder after pulverizing by weight; The weight ratio of described warm water and microbiobacterial agent is 450～500:1.
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| CN106490344A (en) * | 2016-01-13 | 2017-03-15 | 中弘新缘生物科技（天津）有限公司 | A kind of hybrid giant napier silage inoculants bacteria and preparation method thereof |
| CN109302954A (en) * | 2018-11-22 | 2019-02-05 | 中国科学院青岛生物能源与过程研究所 | Utilize southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem |
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