CN102067850A - Application of psoralen as agricultural disinfectant or pesticide - Google Patents
Application of psoralen as agricultural disinfectant or pesticide Download PDFInfo
- Publication number
- CN102067850A CN102067850A CN2011100500884A CN201110050088A CN102067850A CN 102067850 A CN102067850 A CN 102067850A CN 2011100500884 A CN2011100500884 A CN 2011100500884A CN 201110050088 A CN201110050088 A CN 201110050088A CN 102067850 A CN102067850 A CN 102067850A
- Authority
- CN
- China
- Prior art keywords
- psoralen
- insect
- agricultural
- purposes according
- pesticide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 title claims abstract description 81
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 239000000575 pesticide Substances 0.000 title claims abstract description 9
- 239000000645 desinfectant Substances 0.000 title abstract 4
- 241000238631 Hexapoda Species 0.000 claims abstract description 17
- 241000209140 Triticum Species 0.000 claims abstract description 13
- 235000021307 Triticum Nutrition 0.000 claims abstract description 13
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 7
- 241000223218 Fusarium Species 0.000 claims abstract description 7
- 208000031888 Mycoses Diseases 0.000 claims abstract description 7
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract description 6
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 6
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 6
- 240000007594 Oryza sativa Species 0.000 claims abstract description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 6
- 206010039509 Scab Diseases 0.000 claims abstract description 6
- 241000221662 Sclerotinia Species 0.000 claims abstract description 6
- 235000009566 rice Nutrition 0.000 claims abstract description 6
- 239000002917 insecticide Substances 0.000 claims description 13
- 230000000844 anti-bacterial effect Effects 0.000 claims description 9
- 239000003899 bactericide agent Substances 0.000 claims description 9
- 241000500437 Plutella xylostella Species 0.000 claims description 8
- 239000003905 agrochemical Substances 0.000 claims description 8
- 201000010099 disease Diseases 0.000 claims description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 8
- 239000000839 emulsion Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000009472 formulation Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 240000002791 Brassica napus Species 0.000 claims description 5
- 241000409991 Mythimna separata Species 0.000 claims description 5
- 241000255969 Pieris brassicae Species 0.000 claims description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
- 241000272639 Brachycaudus mimeuri Species 0.000 claims description 4
- 241000258937 Hemiptera Species 0.000 claims description 4
- 241000256247 Spodoptera exigua Species 0.000 claims description 4
- 241000985245 Spodoptera litura Species 0.000 claims description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 4
- 239000002671 adjuvant Substances 0.000 claims description 4
- 239000000417 fungicide Substances 0.000 claims description 4
- 239000011593 sulfur Substances 0.000 claims description 4
- 229910052717 sulfur Inorganic materials 0.000 claims description 4
- 241000256259 Noctuidae Species 0.000 claims description 3
- 239000004530 micro-emulsion Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000002728 pyrethroid Substances 0.000 claims description 3
- 238000009736 wetting Methods 0.000 claims description 3
- 241000255777 Lepidoptera Species 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000375 suspending agent Substances 0.000 claims description 2
- 239000003986 organophosphate insecticide Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 230000003405 preventing effect Effects 0.000 abstract description 5
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 231100000053 low toxicity Toxicity 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 abstract 3
- 230000007613 environmental effect Effects 0.000 abstract 2
- 240000000385 Brassica napus var. napus Species 0.000 abstract 1
- 238000000034 method Methods 0.000 description 11
- 239000004480 active ingredient Substances 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000005303 weighing Methods 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 150000004040 pyrrolidinones Chemical class 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical group C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 231100000225 lethality Toxicity 0.000 description 4
- 229920001285 xanthan gum Polymers 0.000 description 4
- 241000813090 Rhizoctonia solani Species 0.000 description 3
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- -1 polyoxyethylene Polymers 0.000 description 3
- 239000013558 reference substance Substances 0.000 description 3
- 239000005660 Abamectin Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000223221 Fusarium oxysporum Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- RRZXIRBKKLTSOM-XPNPUAGNSA-N avermectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 RRZXIRBKKLTSOM-XPNPUAGNSA-N 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 229960002483 decamethrin Drugs 0.000 description 2
- OWZREIFADZCYQD-NSHGMRRFSA-N deltamethrin Chemical compound CC1(C)[C@@H](C=C(Br)Br)[C@H]1C(=O)O[C@H](C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 OWZREIFADZCYQD-NSHGMRRFSA-N 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- BULVZWIRKLYCBC-UHFFFAOYSA-N phorate Chemical compound CCOP(=S)(OCC)SCSCC BULVZWIRKLYCBC-UHFFFAOYSA-N 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 description 1
- RNMDNPCBIKJCQP-UHFFFAOYSA-N 5-nonyl-7-oxabicyclo[4.1.0]hepta-1,3,5-trien-2-ol Chemical compound C(CCCCCCCC)C1=C2C(=C(C=C1)O)O2 RNMDNPCBIKJCQP-UHFFFAOYSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 239000005946 Cypermethrin Substances 0.000 description 1
- 241001046947 Ectropis obliqua Species 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 229960005424 cypermethrin Drugs 0.000 description 1
- KAATUXNTWXVJKI-UHFFFAOYSA-N cypermethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OC(C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 KAATUXNTWXVJKI-UHFFFAOYSA-N 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- OEBRKCOSUFCWJD-UHFFFAOYSA-N dichlorvos Chemical compound COP(=O)(OC)OC=C(Cl)Cl OEBRKCOSUFCWJD-UHFFFAOYSA-N 0.000 description 1
- 229950001327 dichlorvos Drugs 0.000 description 1
- FAXIJTUDSBIMHY-UHFFFAOYSA-N diethoxy-(2-ethylsulfanylethoxy)-sulfanylidene-$l^{5}-phosphane;1-diethoxyphosphorylsulfanyl-2-ethylsulfanylethane Chemical compound CCOP(=O)(OCC)SCCSCC.CCOP(=S)(OCC)OCCSCC FAXIJTUDSBIMHY-UHFFFAOYSA-N 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229960001952 metrifonate Drugs 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- NFACJZMKEDPNKN-UHFFFAOYSA-N trichlorfon Chemical compound COP(=O)(OC)C(O)C(Cl)(Cl)Cl NFACJZMKEDPNKN-UHFFFAOYSA-N 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000004562 water dispersible granule Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of psoralen as an agricultural disinfectant or pesticide. The psoralen serving as the pesticide is used for preventing and controlling lepidopterous and homopterous insects, and the psoralen serving as the disinfectant is used for preventing and controlling agricultural fungus diseases and particularly has obvious preventing and controlling effects on sclerotinia rot of colza, cucumber fusarium wilt, rice sheath blight, wheat scab and wheat sharp eyespot. The psoralen serving as the agricultural disinfectant and pesticide is characterized by good effect, broad spectrum, low toxicity, no residue, environmental friendliness and no public hazard, and is particularly favorable for environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to psoralen prepares agricultural fungicides or bactericide as active component purposes.
Background technology
Pest are taken place in the many regional large tracts of land of China, and frequency is in rising trend, and harm increases the weight of.Still be the major control measure at present with the chemical pesticide control.Because the long-term chemical pesticide control that uses, cause insect that number of chemical insecticides such as organochlorine, organic phosphor, carbamate, pyrethroid have been produced pesticide resistance in various degree, control efficiency significantly descends, make the peasant have to strengthen dosage, still reduced the safety of agricultural product again to improve preventive effect.
In addition, in the agricultural production, the pathogen of damage to crops also has fungi, bacterium, virus etc., having the greatest impact of fungi wherein, and crop production reduction is obvious, because of plant disease especially be can not be ignored by the economic loss that fungus-caused disease causes.
Psoralen, English name: Psoralen, chemical name: 7H-Furo (3,2-g) (1) benzopyran-7-one, molecular weight: 186.16, molecular structure:
Psoralen is colourless acicular crystal (ethanol), and 189～190 ℃ of fusing points are dissolved in ethanol, chloroform, are slightly soluble in water, ether and benzinum.Existing report psoralen acts on human body has an anastalsis to uterus, nose and gum etc. are hemorrhage; Bronchial smooth muscle there is the diastole effect; The leukaemia there is stronger lethal effect (IC50 is 4ug/ml), in addition, also has antitumaous effect.But, the pertinent literature or the report that do not have psoralen on agricultural, to use so far as bactericide or insecticide.
Summary of the invention
The objective of the invention is to explore psoralen as the application of active component at preparation agricultural fungicides or bactericide.
The present invention is by studies have shown that in a large number, and psoralen has excellent insecticidal activity as the agricultural fungicides that active component prepares, and can be used for preventing and treating various pest on crop, especially for control Lepidoptera and Homoptera insect.Lepidopterous insects can be leaf (night) moth section, diamond-back moth section, Sulfur butterfly insect; Described noctuid is preferably prodenia litura, beet armyworm, mythimna separata; Diamond-back moth section insect is preferably diamond-back moth; The Sulfur butterfly insect is preferably cabbage caterpillar.Described Homoptera insect is the Aphidiadae insect, is preferably black peach aphid.
The agricultural bacteriocide that psoralen prepares as active component also has excellent bactericidal activity, can be applicable to the disease on the various crops of control, be particularly suitable for preventing and treating agricultural fungal diseases, as: sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, wheat sharp eyespot.
Psoralen is that separation and purification obtains or commercially available getting from natural plants.Psoralen is mixed with any one formulation that allows on the agricultural chemicals as active component and insecticides adjuvant, excipients.All right and other agriculture chemical compounding of psoralen is mixed and made into any one formulation that allows on the agricultural chemicals with insecticides adjuvant, excipients.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Used auxiliary agent can adopt calcium dodecyl benzene sulfonate, pyrrolidones, polyoxyethylene polyoxy third rare block compound, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other used agricultural chemicals are organic phosphates, as: thimet (3911), demeton (1059, E1059), parathion (1605, E605), dichlorvos (DDV), Rogor, chlorophos etc.; Pyrethroid, as: cypermethrin, decamethrin, kill bacterium fat etc.; The microbial source insecticide is as bacillus thuringiensis,Bt (Bt), Avermectin, white muscardine fungi etc.
Beneficial effect of the present invention compared with the prior art: psoralen is as insecticide or bactericide, and insecticidal spectrum, antimicrobial spectrum are wide, and desinsection, antibacterial time are short, and the lasting period is long, is insecticide, the bactericide of agriculture coideal.Compare with general chemical pesticide; insect had tag, stomach poison function, effect is remarkable, and psoralen derives from the extract of natural plants; have low toxicity, noresidue, pollution-free, non-harmful characteristics for economic crops such as various grains, melon and fruit, vegetables, more favorable environment protection.
Embodiment
Below by embodiment effect of the present invention is described further:
Embodiment 1, psoralen are to the control of agricultural fungal diseases
Strains tested Fusarium graminearum (Fusarium.graminearm) obtains from the separation of wheat scab fringe; Fusarium oxysporum bacterium (F.oxysporum) obtains from the separation of cucumber fusarium axysporum diseased plant; Rhizoctonia solani Kuhn (Rhizoctonia solani) obtains from the separation of rice sheath blight disease leaf; Cereal rhizoctonia (Rhizoctonia solani) obtains from the separation of wheat sharp eyespot leaf; Sclerotinite (Sclerotioia selerotiorum) obtains from the separation of sclerotinia rot of colza bacterial strain.Reagent agent psoralen (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute) is dissolved in acetone with it and makes 1000 μ g/mL mother liquors, and 4 ℃ of preservations are standby.PDA medium: 200g peeling potato is cut into piece, boil 10min after, get juice, add 20g agar, be heated to fusing, add 20g glucose again, water is settled to 1000mL.
Adopt the mycelial growth rate determination method that the inhibition of hypha,hyphae growth is measured, pre-incubated fungi edge is beaten and is got bacterium dish (diameter 5mm) from the PDA flat board, the reagent agent mother liquor is evenly mixed with medium, make that the reagent agent ultimate density is respectively 0,1,2.5,5,10ppm, mixed liquor impouring diameter respectively is to make flat board in the sterilization culture dish of 9cm, every ware is inoculated a bacterium dish, and mycelia faces up, and every processing repeats 3 times.25 ℃ are cultured to the contrast bacterium colony and are covered with culture dish 2/3rds when above, and the right-angled intersection method is measured bacterium colony growth diameter.Calculate growth inhibition ratio by colony diameter mean value (mm).Data to gained are handled with DPS V3.01 professional version software analysis, draw calibration curve and EC
50Value.Positive reference substance is Osthole (available from a Nat'l Pharmaceutical ﹠ Biological Products Control Institute), calculates the conk inhibiting rate with same concentration with quadrat method.
Psoralen can be prevented and treated sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, wheat sharp eyespot effectively as can be seen from the result, concentration EC during these several fungal diseases are suppressed
50Be 5.46～28.71ppm, concrete experimental result sees Table 1; And the positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, these 5 kinds of fungal diseases inhibition of wheat sharp eyespot
50Be respectively 43.33,41.82,26.84,56.94,39.96ppm, demonstrated fully the remarkable result of agricultural chemicals of the present invention by the contrast experiment.
Table 1 psoralen is to agriculture fungi toxicity test result
Embodiment 2 psoralens as active component to agricultural insect management
Lay eggs to pupating in indoor artificial feed raising for field population 3～6 instar larvaes of gathering for trying the worm source, select indoor F1 and F2 and use (wherein, cabbage caterpillar is that population is gathered in the field) for toxicity test for 2～3 instar larvaes of big or small basically identical.Accurately take by weighing psoralen (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute), it is dissolved in acetone, making concentration is the confession examination mother liquor of 2.000mg/ml, is diluted to 6 different concentration as standby for reagent liquid with the 1%TritonX-100 aqueous emulsion then.With defoliator (wherein with fresh clean cabbage leaves, mythimna separata is a fresh tea leaf in its with wheat leaf blade, tea geometrid) make the leaf dish that diameter is 1.5cm, take out after then it being soaked 10 seconds in for reagent liquid, the back is taken out to dry and is placed the culture dish that contains the 1% water agar or the filter paper of preserving moisture, insert the examination worm of hungry 3h, place artificial climate indoor [(25 ± 1) ℃, L/D=14/10, RH 75%], the soup of each concentration is handled 30 cephalonts.The blank group adds 1%Triton100 aqueous emulsion dipping blade with acetone, and positive reference substance is Osthole (available from a Nat'l Pharmaceutical ﹠ Biological Products Control Institute), handles the examination worm with the same leaf dipping method of same concentration.48h checks lethality, calculates each dead borer population of handling, borer population alive, calculates larval mortality.Calculate LC with the biometric counting according to handling with management system software Polo-Plus program (LeOra Software 2002)
50Value.
The result shows: in the 48h, the psoralen of variable concentrations is effective to prodenia litura, beet armyworm, diamond-back moth, cabbage caterpillar, mythimna separata, black peach aphid, concrete lethality and LC
50See Table 2; And positive controls, the lethality that examination worm prodenia litura, beet armyworm, mythimna separata, the black peach aphid that concentration is handled for the 1.000mg/ml Osthole tags is all less than 20%, and the examination worm cabbage caterpillar that concentration is handled for the 2.000mg/ml Osthole, the lethality of diamond-back moth are respectively 80%, 70%.Show that by comparative trial psoralen has the advantage that the desinsection time is short, the desinsection scope is wide as agricultural insecticide.
Table 2 psoralen is to the virulence test result of insect
Embodiment 3:
Take by weighing conventional auxiliary materials such as psoralen and lignin, diatomite according to common process, make active ingredient concentration and be 2% wetting powder.
Embodiment 4:
Take by weighing psoralen and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, the conventional auxiliary material of solvent wet goods according to common process, make active ingredient concentration and be 2% missible oil.
Embodiment 5:
Claim conventional auxiliary materials such as psoralen and wooden sodium sulfonate, kaolin according to common process, make active ingredient concentration and be 2% water dispersible granules.
Embodiment 6:
Take by weighing psoralen and solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 1% aqueous emulsion.
Embodiment 7:
Take by weighing conventional auxiliary materials such as psoralen and calcium dodecyl benzene sulfonate, pyrrolidones according to common process, make active ingredient concentration and be 1% microemulsion.
Embodiment 8:
Take by weighing psoralen and thimet according to 1: 5 ratio of weight ratio, with solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 6% aqueous emulsion.
Embodiment 9:
Take by weighing psoralen and decamethrin according to 6: 1 ratio of weight ratio, with solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 7% aqueous emulsion.
Embodiment 10:
Take by weighing psoralen and Avermectin according to 1: 3 ratio of weight ratio, with solvent naphtha, pyrrolidones, conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 4% aqueous emulsion.
Claims (10)
1. psoralen is as the purposes of active component in preparation agricultural fungicides or bactericide.
2. purposes according to claim 1 is characterized in that psoralen is mixed with any one formulation that allows on the agricultural chemicals as active component and insecticides adjuvant, excipients; Perhaps psoralen is mixed with any one formulation that allows on the agricultural chemicals as active component and other agricultural chemicals and insecticides adjuvant, excipients.
3. purposes according to claim 2 is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Described other agricultural chemicals are organophosphorus insecticide, pyrethroid pesticide or microbial source insecticide.
4. purposes according to claim 1 is characterized in that described insecticide is used to prevent and treat Lepidoptera and Homoptera insect.
5. purposes according to claim 4 is characterized in that described lepidopterous insects is noctuid, diamond-back moth section insect, Sulfur butterfly insect.
6. purposes according to claim 5 is characterized in that described noctuid is prodenia litura, beet armyworm, mythimna separata; Described diamond-back moth section insect is a diamond-back moth; Described Sulfur butterfly insect is a cabbage caterpillar.
7. purposes according to claim 4 is characterized in that described Homoptera insect is the Aphidiadae insect.
8. purposes according to claim 7 is characterized in that described Aphidiadae insect is a black peach aphid.
9. purposes according to claim 1 is characterized in that psoralen is used to prevent and treat agricultural fungal diseases as bactericide.
10. application according to claim 9 is characterized in that described agricultural fungal diseases is sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, wheat sharp eyespot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110050088 CN102067850B (en) | 2011-03-02 | 2011-03-02 | Application of psoralen as agricultural disinfectant or pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110050088 CN102067850B (en) | 2011-03-02 | 2011-03-02 | Application of psoralen as agricultural disinfectant or pesticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102067850A true CN102067850A (en) | 2011-05-25 |
| CN102067850B CN102067850B (en) | 2013-07-17 |
Family
ID=44026757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110050088 Expired - Fee Related CN102067850B (en) | 2011-03-02 | 2011-03-02 | Application of psoralen as agricultural disinfectant or pesticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102067850B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105646430A (en) * | 2016-03-28 | 2016-06-08 | 石鸿娟 | Natural product capable of being developed into agricultural sterilizing agent and preparation method thereof |
| CN107484766A (en) * | 2017-08-29 | 2017-12-19 | 郑州市汇川农业科技有限公司 | Agricultural chemicals and preparation, application process based on natural cumarin essential oil in common fig leaf juice |
| CN107743970A (en) * | 2017-12-06 | 2018-03-02 | 惠州市支持网知识产权运营有限公司 | Bactericidal composition containing psoralen and gold alloy hydride |
| CN107787995A (en) * | 2017-12-06 | 2018-03-13 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of Pesticidal combination and its application |
| KR20180037979A (en) * | 2015-07-31 | 2018-04-13 | 센트로 데 인제니에리아 제네티카 와이 바이오테크놀로지아 | How to Treat Plant Diseases |
| CN107912440A (en) * | 2017-12-06 | 2018-04-17 | 赖进九 | A kind of Pesticidal combination and its application |
| CN107950550A (en) * | 2017-12-11 | 2018-04-24 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN107950549A (en) * | 2017-12-06 | 2018-04-24 | 温妙云 | A kind of Pesticidal combination |
| CN108477187A (en) * | 2018-04-16 | 2018-09-04 | 杜桂香 | A kind of bactericidal composition containing psoralen and coumoxystrobin |
| CN108633893A (en) * | 2018-04-16 | 2018-10-12 | 张友兰 | Bactericidal composition containing psoralen and dimoxystrobin |
| CN108633894A (en) * | 2018-04-16 | 2018-10-12 | 赖振明 | A kind of bactericidal composition containing psoralen and Nuo Wo mycins |
| CN108633892A (en) * | 2018-04-16 | 2018-10-12 | 张月婵 | Bactericidal composition containing psoralen and octyl trichlamide |
| CN109090160A (en) * | 2018-09-14 | 2018-12-28 | 安徽省益农化工有限公司 | A kind of bactericidal aqueous emulsion of the environment-friendly type containing a variety of Chinese medicine composition |
| CN110178849A (en) * | 2019-05-24 | 2019-08-30 | 江苏省中国科学院植物研究所 | Purposes of the psoralen in prevention and treatment phytobacterial disease |
| CN113929696A (en) * | 2021-11-16 | 2022-01-14 | 南开大学 | Acylthiourea psoralen derivatives, and preparation method and application thereof |
| CN113929694A (en) * | 2021-11-16 | 2022-01-14 | 南开大学 | Sulfonyl hydrazide psoralen derivatives, and preparation method and application thereof |
| CN115005226A (en) * | 2022-06-02 | 2022-09-06 | 广西壮族自治区农业科学院 | Insecticidal composition for preventing and treating corn armyworm |
| CN117256632A (en) * | 2023-09-20 | 2023-12-22 | 南京金色庄园农产品有限公司 | Biological combined medicament for preventing and treating powdery mildew of fruits and vegetables and high-efficiency prevention and treatment method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101213980A (en) * | 2008-01-02 | 2008-07-09 | 沈阳化工学院 | Biological pesticide using psoralidin to prevent and control plant fungal diseases |
-
2011
- 2011-03-02 CN CN 201110050088 patent/CN102067850B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101213980A (en) * | 2008-01-02 | 2008-07-09 | 沈阳化工学院 | Biological pesticide using psoralidin to prevent and control plant fungal diseases |
Non-Patent Citations (2)
| Title |
|---|
| PSRAST: "《Genetically Engineered Food - Safety Problems》", 1997, article "Plant-Pesticides in GE-food-A potential health risk" * |
| 胡丽丽等: "无花果叶中抑菌活性成分-补骨脂素的研究", 《青岛农业大学学报(自然科学版)》, vol. 24, no. 4, 2007, pages 266 * |
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108135176A (en) * | 2015-07-31 | 2018-06-08 | 遗传工程与生物技术中心 | For treating the method for plant disease |
| CN108135176B (en) * | 2015-07-31 | 2021-06-04 | 遗传工程与生物技术中心 | Method for treating plant diseases |
| US11013235B2 (en) | 2015-07-31 | 2021-05-25 | Centro De Ingeniería Genética Y Biotecnología | Method for treating diseases in plants |
| KR20180037979A (en) * | 2015-07-31 | 2018-04-13 | 센트로 데 인제니에리아 제네티카 와이 바이오테크놀로지아 | How to Treat Plant Diseases |
| US10993438B2 (en) | 2015-07-31 | 2021-05-04 | Centro De Ingeniería Genética Y Biotecnología | Method for treating diseases in plants |
| RU2739944C2 (en) * | 2015-07-31 | 2020-12-30 | Сентро Де Инхеньерия Хенетика И Биотекнолохия | Method of treating plant diseases |
| CN105646430B (en) * | 2016-03-28 | 2018-01-09 | 温岭市创嘉信息科技有限公司 | A kind of natural products that can develop into agricultural bacteriocide and preparation method thereof |
| CN105646430A (en) * | 2016-03-28 | 2016-06-08 | 石鸿娟 | Natural product capable of being developed into agricultural sterilizing agent and preparation method thereof |
| CN107484766A (en) * | 2017-08-29 | 2017-12-19 | 郑州市汇川农业科技有限公司 | Agricultural chemicals and preparation, application process based on natural cumarin essential oil in common fig leaf juice |
| CN107912440A (en) * | 2017-12-06 | 2018-04-17 | 赖进九 | A kind of Pesticidal combination and its application |
| CN107743970A (en) * | 2017-12-06 | 2018-03-02 | 惠州市支持网知识产权运营有限公司 | Bactericidal composition containing psoralen and gold alloy hydride |
| CN107950549A (en) * | 2017-12-06 | 2018-04-24 | 温妙云 | A kind of Pesticidal combination |
| CN107787995A (en) * | 2017-12-06 | 2018-03-13 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of Pesticidal combination and its application |
| CN107950550A (en) * | 2017-12-11 | 2018-04-24 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN108633893A (en) * | 2018-04-16 | 2018-10-12 | 张友兰 | Bactericidal composition containing psoralen and dimoxystrobin |
| CN108633894A (en) * | 2018-04-16 | 2018-10-12 | 赖振明 | A kind of bactericidal composition containing psoralen and Nuo Wo mycins |
| CN108633892A (en) * | 2018-04-16 | 2018-10-12 | 张月婵 | Bactericidal composition containing psoralen and octyl trichlamide |
| CN108477187A (en) * | 2018-04-16 | 2018-09-04 | 杜桂香 | A kind of bactericidal composition containing psoralen and coumoxystrobin |
| CN109090160A (en) * | 2018-09-14 | 2018-12-28 | 安徽省益农化工有限公司 | A kind of bactericidal aqueous emulsion of the environment-friendly type containing a variety of Chinese medicine composition |
| CN110178849B (en) * | 2019-05-24 | 2021-03-23 | 江苏省中国科学院植物研究所 | Application of psoralen in preventing and treating plant bacterial diseases |
| CN110178849A (en) * | 2019-05-24 | 2019-08-30 | 江苏省中国科学院植物研究所 | Purposes of the psoralen in prevention and treatment phytobacterial disease |
| CN113929696A (en) * | 2021-11-16 | 2022-01-14 | 南开大学 | Acylthiourea psoralen derivatives, and preparation method and application thereof |
| CN113929694A (en) * | 2021-11-16 | 2022-01-14 | 南开大学 | Sulfonyl hydrazide psoralen derivatives, and preparation method and application thereof |
| CN113929696B (en) * | 2021-11-16 | 2024-05-14 | 南开大学 | Acyl thiourea psoralen derivatives, and preparation method and application thereof |
| CN115005226A (en) * | 2022-06-02 | 2022-09-06 | 广西壮族自治区农业科学院 | Insecticidal composition for preventing and treating corn armyworm |
| CN117256632A (en) * | 2023-09-20 | 2023-12-22 | 南京金色庄园农产品有限公司 | Biological combined medicament for preventing and treating powdery mildew of fruits and vegetables and high-efficiency prevention and treatment method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102067850B (en) | 2013-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102067850B (en) | Application of psoralen as agricultural disinfectant or pesticide | |
| Edirisinghe et al. | Chitosan controls postharvest anthracnose in bell pepper by activating defense-related enzymes | |
| CN104531574B (en) | A kind of bacillus amyloliquefaciens gfj 4 and combinations thereof | |
| CN106070338B (en) | A kind of compounding agricultural insecticidal bactericidal composition and its application | |
| CN104322558A (en) | Sterilization composition and applications thereof | |
| MARQUES et al. | Antimicrobial and insecticidal activities of cashew tree gum exudate | |
| CN1327770C (en) | Sterilization composition containing carbendazim of multiple antimycin | |
| CN102657235B (en) | Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof | |
| CN103704224A (en) | Shenqinmycin-avermectins suspoemulsion used for controlling plant nematodes and preparation method of the suspoemulsion | |
| KR101837622B1 (en) | Composition for controlling plant diseases comprising an extract of Maesa japonica, and method for controlling plant diseases using the same | |
| CN106857581A (en) | A kind of nematicidal composition containing trifluoro miaow pyridine acid amides | |
| CN105340892B (en) | A kind of purposes of native compound murrayone as agricultural bacteriocide | |
| CN103109821B (en) | Use of Columbianetin as agricultural pesticide | |
| CN103858874B (en) | Application of natural compound as agricultural bactericide | |
| Laznik et al. | Efficacy of three natural substances against apple aphid (Aphis pomi De Geer, Aphididae, Homoptera) under laboratory conditions | |
| CN105028435B (en) | A kind of native compound is as the purposes of agricultural bacteriocide | |
| CN103749546A (en) | Isaria fumosorosea and beta-cypermethrin oil suspension and applications thereof | |
| CN103190405B (en) | Use of natural compound as agricultural bactericide or pesticide | |
| CN103109822B (en) | Application of byakangelicin used as agricultural insect antifeedant and pesticide | |
| CN104996458A (en) | Pesticide composition and applications thereof | |
| CN105432631B (en) | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide | |
| Modi et al. | Use of urine and dung samples of cow and goat for biological control of phytopathogenic fungus Colletrotrichum falcatum | |
| CN103109820B (en) | Use of columbianadin as agricultural insect antifeedant and pesticide | |
| CN103141491B (en) | Applications of natural compound as agriculture bactericide | |
| CN107006499A (en) | Line style pyranocoumarin compound Xanthyletine as agricultural bacteriocide purposes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130717 Termination date: 20150302 |
|
| EXPY | Termination of patent right or utility model |