CN101935647A - 一种提取微生物dna的试剂盒及其方法 - Google Patents
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Abstract
Description
本发明方法 | Mo Bio UltraClean Soil DNA Kit | |
DNA浓度(μg/ml) | 104 | 86 |
OD260/OD280 | 1.7 | 1.86 |
OD260/OD230 | 0.94 | 0.87 |
预变性 | 变性 | 退火 | 延伸 | 末次延伸 |
94℃3min | 94℃45s | 50℃45s | 72℃1min30s,35个循环 | 72℃10min |
Claims (10)
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Cited By (27)
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CN103649298A (zh) * | 2011-05-12 | 2014-03-19 | 精密科学公司 | 核酸的分离 |
CN104178481A (zh) * | 2014-09-05 | 2014-12-03 | 福建师范大学 | 一种30分钟内提取土壤微生物基因组dna的试剂盒 |
CN104531680A (zh) * | 2014-12-29 | 2015-04-22 | 福建师范大学 | 快速提取动物粪便微生物基因组dna的试剂盒及提取方法 |
CN104560953A (zh) * | 2014-12-29 | 2015-04-29 | 福建师范大学 | 快速提取淤泥微生物基因组dna的试剂盒及提取方法 |
CN104560951A (zh) * | 2014-12-03 | 2015-04-29 | 复旦大学泰州健康科学研究院 | 一种宏基因组dna的提取方法及其试剂盒 |
CN105176970A (zh) * | 2015-07-16 | 2015-12-23 | 北京百迈客生物科技有限公司 | 一种适用于高通量测序的样品中微生物dna提取方法 |
CN105821036A (zh) * | 2016-06-03 | 2016-08-03 | 南京大学 | 一种蚯蚓肠道微生物总dna的提取方法 |
CN106381344A (zh) * | 2016-09-07 | 2017-02-08 | 林勤 | 一种基于ddPCR的高灵敏EBV DNA定量检测试剂盒及使用方法 |
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CN106754874A (zh) * | 2016-12-06 | 2017-05-31 | 浙江指针基因科技有限公司 | 一种从人体粪便样本中提取dna的试剂盒及其使用方法 |
CN106906208A (zh) * | 2017-05-04 | 2017-06-30 | 廊坊恒益生物技术有限公司 | 一种高效提取核酸的方法 |
CN107151666A (zh) * | 2016-03-03 | 2017-09-12 | 上海市农业科学院 | 一种水体中微生物dna的提取方法 |
CN108342382A (zh) * | 2018-01-30 | 2018-07-31 | 广东海大畜牧兽医研究院有限公司 | 一种核酸快速提取方法及其试剂盒 |
CN108368539A (zh) * | 2015-09-10 | 2018-08-03 | 生命技术公司 | 环境或生物样本中的核酸纯化 |
CN109266642A (zh) * | 2017-07-18 | 2019-01-25 | 天根生化科技(北京)有限公司 | 磁珠法提取粪便微生物基因组的试剂盒及提取方法 |
WO2019180519A1 (en) * | 2018-03-17 | 2019-09-26 | Metagenom Bio Holdings Pte Ltd. | Method and kit for isolation of nucleic acids from a sample |
WO2020014458A1 (en) * | 2018-07-11 | 2020-01-16 | Monsanto Technology Llc | Extraction of polynucleotides |
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CN113088516A (zh) * | 2021-05-13 | 2021-07-09 | 张帮周 | 用于粪便微生物基因组的dna提取试剂盒及提取方法 |
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CN113717970A (zh) * | 2021-10-15 | 2021-11-30 | 苏州中科先进技术研究院有限公司 | 一种粪便基因组提取试剂盒及使用方法 |
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CN114369594A (zh) * | 2022-02-22 | 2022-04-19 | 上海派森诺生物科技有限公司 | 一种粪便内微生物的总dna的提取试剂盒和提取方法 |
CN115058415A (zh) * | 2022-08-18 | 2022-09-16 | 南京集思慧远生物科技有限公司 | 一种快速、高质量、通用型基因组dna提取试剂盒及dna提取方法 |
CN115532242A (zh) * | 2022-10-18 | 2022-12-30 | 广州美基生物科技有限公司 | 腐殖酸吸附剂及其制备方法土壤dna提取试剂盒 |
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CN1990863A (zh) * | 2005-12-30 | 2007-07-04 | 华中农业大学 | 一种土壤总dna小量快速提取方法 |
CN101372689A (zh) * | 2007-08-22 | 2009-02-25 | 中国科学院沈阳应用生态研究所 | 一种土壤微生物基因组dna提取方法 |
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CN1936005A (zh) * | 2006-08-29 | 2007-03-28 | 中国科学院南海海洋研究所 | 从土壤中提取微生物基因组dna的试剂盒及方法 |
CN101372689A (zh) * | 2007-08-22 | 2009-02-25 | 中国科学院沈阳应用生态研究所 | 一种土壤微生物基因组dna提取方法 |
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CN105821036A (zh) * | 2016-06-03 | 2016-08-03 | 南京大学 | 一种蚯蚓肠道微生物总dna的提取方法 |
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CN106754874A (zh) * | 2016-12-06 | 2017-05-31 | 浙江指针基因科技有限公司 | 一种从人体粪便样本中提取dna的试剂盒及其使用方法 |
CN106906208A (zh) * | 2017-05-04 | 2017-06-30 | 廊坊恒益生物技术有限公司 | 一种高效提取核酸的方法 |
CN109266642A (zh) * | 2017-07-18 | 2019-01-25 | 天根生化科技(北京)有限公司 | 磁珠法提取粪便微生物基因组的试剂盒及提取方法 |
CN108342382B (zh) * | 2018-01-30 | 2020-12-15 | 广东海大畜牧兽医研究院有限公司 | 一种核酸快速提取方法及其试剂盒 |
CN108342382A (zh) * | 2018-01-30 | 2018-07-31 | 广东海大畜牧兽医研究院有限公司 | 一种核酸快速提取方法及其试剂盒 |
WO2019180519A1 (en) * | 2018-03-17 | 2019-09-26 | Metagenom Bio Holdings Pte Ltd. | Method and kit for isolation of nucleic acids from a sample |
WO2020014458A1 (en) * | 2018-07-11 | 2020-01-16 | Monsanto Technology Llc | Extraction of polynucleotides |
CN112195177A (zh) * | 2020-10-28 | 2021-01-08 | 上海慕柏生物医学科技有限公司 | 一种核酸提取方法及试剂盒 |
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