CN101903104A - 综合微液体装置及其方法 - Google Patents
综合微液体装置及其方法 Download PDFInfo
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- CN101903104A CN101903104A CN2008801204884A CN200880120488A CN101903104A CN 101903104 A CN101903104 A CN 101903104A CN 2008801204884 A CN2008801204884 A CN 2008801204884A CN 200880120488 A CN200880120488 A CN 200880120488A CN 101903104 A CN101903104 A CN 101903104A
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- B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
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- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
- B01F25/452—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces
- B01F25/4521—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces the components being pressed through orifices in elements, e.g. flat plates or cylinders, which obstruct the whole diameter of the tube
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- B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
- B01F31/651—Mixing by successively aspirating a part of the mixture in a conduit, e.g. a piston, and reinjecting it through the same conduit into the receptacle
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- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1816—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using induction heating
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
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- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
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- Hematology (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
试剂 | 容量(μL) | 意见 |
样本 | 20 | |
ATL | 20 | 细胞裂解缓冲剂 |
蛋白脢K | 3 | |
AL | 40 | Si膜粘合缓冲剂 |
乙醇 | 40 | 帮助将膜干燥 |
AW1 | 50 | 清洗缓冲剂1 |
AW2 | 50 | 清洗缓冲剂2 |
AE | 50 | DNA洗脱缓冲剂 |
在20μL样本内装载的微生物数量 | 在PCR间隔内相等的基因组装载数量 |
500,000 | 20,000 |
100,000 | 4,000 |
2,500 | 100 |
行号 | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
试验 | 负,苹果汁 | 正,苹果汁 | 正,苹果汁 | 负,奶 | 正,奶 | 正,奶 | 西打 |
Claims (44)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US97951507P | 2007-10-12 | 2007-10-12 | |
US60/979,515 | 2007-10-12 | ||
PCT/US2008/079659 WO2009049268A1 (en) | 2007-10-12 | 2008-10-10 | Integrated microfluidic device and methods |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101903104A true CN101903104A (zh) | 2010-12-01 |
CN101903104B CN101903104B (zh) | 2014-06-25 |
Family
ID=40251703
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200880120488.4A Active CN101903104B (zh) | 2007-10-12 | 2008-10-10 | 综合微流体装置及其方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US9132398B2 (zh) |
EP (1) | EP2265375A1 (zh) |
JP (1) | JP5523327B2 (zh) |
CN (1) | CN101903104B (zh) |
WO (1) | WO2009049268A1 (zh) |
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CN105214744A (zh) * | 2015-10-26 | 2016-01-06 | 深圳华迈兴微医疗科技有限公司 | 一种磁微粒化学发光微流控芯片 |
CN107198341A (zh) * | 2016-09-26 | 2017-09-26 | 梁继森 | 美容液可控面部美容器 |
CN107384776A (zh) * | 2017-08-04 | 2017-11-24 | 深圳市合川医疗科技有限公司 | 微流控芯片 |
CN108722505A (zh) * | 2018-05-11 | 2018-11-02 | 石家庄禾柏生物技术股份有限公司 | 一种三明治结构的试剂盘 |
CN109957492A (zh) * | 2017-12-26 | 2019-07-02 | 安诺优达基因科技(北京)有限公司 | 一种用于二代测序dna文库构建的自动化液体处理工作站 |
CN110257240A (zh) * | 2019-07-16 | 2019-09-20 | 东莞博识生物科技有限公司 | 核酸提取、扩增及检测一体试剂卡 |
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CN113145185A (zh) * | 2021-02-08 | 2021-07-23 | 北京理工大学 | 一种模块化的微流控核酸检测芯片及*** |
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WO2009049268A1 (en) | 2009-04-16 |
US9132398B2 (en) | 2015-09-15 |
US20120115738A1 (en) | 2012-05-10 |
EP2265375A1 (en) | 2010-12-29 |
JP5523327B2 (ja) | 2014-06-18 |
CN101903104B (zh) | 2014-06-25 |
JP2011501665A (ja) | 2011-01-13 |
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