CN115650793A - Drought-resistant water-retention microbial agent and preparation method thereof - Google Patents
Drought-resistant water-retention microbial agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a drought-resistant water-retaining microbial agent and a preparation method thereof, belonging to the technical field of agricultural microbial agents. The microbial agent is prepared from the following raw materials: carrier, humic acid, biochar, urea, monoammonium phosphate, monopotassium phosphate, water-retaining drought-resisting microbial agent and water-retaining agent. According to the invention, firstly, the polymer composite hydrogel is used as a main water-retaining agent, so that long-acting water retention is realized, the propagation condition of the microbial agent in a complex environment is improved, and the effect of protecting and stabilizing the microbial agent is achieved. Meanwhile, substances such as humic acid, urea and the like are supplemented, so that rich nutrition is provided for the growth of crops, the growth of the crops is promoted, and the final purpose of increasing both production and income is achieved. The water-retaining agent can be applied to water and soil conservation in various environments, has important value for reducing water and soil loss and forming stable ecological environment, and has remarkable economic value and social value.
Description
Technical Field
The invention belongs to the technical field of agricultural microbial agents, and particularly relates to a drought-resistant water-retaining microbial agent and a preparation method thereof.
Background
In the present agricultural field, water resources are an important problem, the agricultural water waste phenomenon is very serious, more than half of the effective irrigation areas still use the traditional backward irrigation method, so that the agricultural water utilization efficiency is not high, and the utilization rate is low. The problem that the shortage of agricultural water is difficult to solve is solved by simply adding a new water source, and the cost is high, so that the scientific planning of the utilization form of water resources becomes one of effective solutions.
The artificial fertilization is an important measure for improving the soil structure and function, but the traditional compound fertilizer has lower fertilizer efficiency and short effective period. The soil water-retaining agent is used as a novel polymer material, the research on the effect of the special physical structure and chemical composition in soil erosion control is deepened more and more, and the soil water-retaining agent has wide application prospects in the aspects of water and fertilizer conservation, drought resistance and water retention of farmlands, crop yield increase and the like.
The water-retaining agent is applied to a high polymer material with better water-retaining and soil-improving effects in the dry farming agricultural production, has better effects on keeping the soil moisture of the dry farming farmland, improving the soil quality and promoting the yield increase of crops, can effectively improve the carbon and nitrogen contents of soil microorganisms, has a certain relation with the application mode and the application amount of the soil microorganisms, can promote the activity improvement of soil sucrase, protease, polyphenol oxidase, urease and the like, and is favorable for the circulation and conversion of nitrogen and carbon.
At present, the water retention agents commonly used internationally are starch graft acrylate copolymerization cross-linked substances and acrylic acid amine-acrylic acid salt copolymerization cross-linked substances. However, the water-retaining agents have the problems of slow degradation speed, fast failure, soil pollution and the like. In recent years, the development of biological water retention agents has become a research hotspot and proves that the biological water retention agents have obvious effects on improving the soil structure and improving the water storage capacity of the soil.
The existing biological water-retaining agents have the problems of unstable water-retaining effect and single function, and simultaneously need to be applied to soil for a plurality of times, so that the planting cost of farmers is increased, and the burden of the soil is increased to a certain degree, therefore, the biological water-retaining agents are difficult to be effectively applied and popularized in actual production.
Disclosure of Invention
Aiming at the problems in the prior art, the invention develops the high-performance biological water-retaining agent, on one hand, the water-retaining effect of the water-retaining agent is greatly improved, the soil structure and the ecological activity are improved, and on the other hand, the drought resistance of crops is improved, so that the yield and income are increased.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
a drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 40-50 parts of carrier, 40-50 parts of humic acid, 10-20 parts of charcoal, 5-10 parts of urea, 5-8 parts of monoammonium phosphate, 2-3 parts of monopotassium phosphate, 2-6 parts of water-retention and drought-resistant microbial agent and 4-8 parts of water-retaining agent.
Further, the carrier is attapulgite and/or diatomite.
Further, the biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
Further, the water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The Bacillus flexus is purchased from the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC1.15227; the bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) and has a preservation number of CGMCC1.14985.
Further, the preparation method of the water-retention drought-resistant microbial agent comprises the following steps: unfreezing the curvating bacillus and the bacillus subtilis respectively, then coating the unfrozen curvating bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12-24h at 28-30 ℃ to obtain activated curvating bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24-48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
Furthermore, the viable count of the curvulus bacteria liquid is (1-1.5) multiplied by 10 10 cfu/mL; the viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL。
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, and the concentration of the extract is 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
Furthermore, the preparation method of the water-retaining agent comprises the following steps: dispersing 10-15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at the rotating speed of 1000-2000r/min for dissolving, adding 1-5g of urea and 1-5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40-60min, then dropwise adding 5-10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3-5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to be neutral, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retaining drought-resisting microbial powder: unfreezing the curvating bacillus and the bacillus subtilis respectively, then coating the unfrozen curvating bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12-24h at 28-30 ℃ to obtain activated curvating bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24-48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain a water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 10-15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at the rotating speed of 1000-2000r/min for dissolving, adding 1-5g of urea and 1-5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40-60min, then dropwise adding 5-10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3-5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to be neutral, and then drying in vacuum and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
Furthermore, the dosage of the drought-resistant water-retention microbial agent is 5-10 kg/mu, and the microbial agent is applied together with a base fertilizer.
The raw materials used in the invention are commercially available.
The preparation method takes an inorganic porous material as a carrier, loads a water retention agent and a biological agent, and is matched with a proper amount of nutrient substances such as humic acid, urea and the like, so that the water retention and the growth promotion are realized, and meanwhile, the nutrient substances are provided for crops, thereby achieving multiple purposes.
According to the invention, the carboxymethyl cellulose and polyacrylamide are used for preparing the composite hydrogel water-retaining agent, the obtained water-retaining agent can realize high-efficiency water retention, and meanwhile, the material can effectively adsorb and protect the microbial agent, so that the microbial agent is protected while the high-efficiency water retention is realized, the stable exertion of the microbial agent is promoted, the survival property and the anti-reverse activity of the microbial agent are improved, and the final purposes of increasing the yield and the income are realized.
The invention uses Bacillus flexus with the preservation number of CGMCC1.15227 and Bacillus subtilis with the preservation number of CGMCC1.14985 to ferment to obtain the microbial agent. The two have synergistic effect, and play the roles of resisting drought and promoting growth. Firstly, the curvularia lunata can increase the content of proline (Pro) to remove Malondialdehyde (MDA) accumulated in cells and enhance the capability of plants in resisting drought stress, thereby improving the adaptability of crops to drought environment. The bacillus subtilis can secrete active substances such as biological enzyme and the like, has stress resistance on one hand, can degrade nutrient substances and promote the bacillus curvatus to play a role on the other hand, and the bacillus curvatus act together to fundamentally improve the drought resistance of crops.
Advantageous effects
The biological water-retaining agent firstly takes the polymer composite hydrogel as a main water-retaining agent, on one hand, long-acting water retention is realized, on the other hand, the propagation condition of a microbial agent in a complex environment is improved, and the function of protecting and stabilizing the microbial agent is achieved. Meanwhile, substances such as humic acid, urea and the like are supplemented, so that rich nutrition is provided for the growth of crops, the growth of the crops is promoted, and the final purpose of increasing both production and income is achieved. The water-retaining agent can be applied to water and soil conservation in various environments, has important value for reducing water and soil loss and forming stable ecological environment, and has remarkable economic value and social value.
Detailed Description
The technical solution of the present invention is further described below with reference to specific embodiments, but is not limited thereto.
Example 1
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 40 parts of carrier, 40 parts of humic acid, 10 parts of charcoal, 5 parts of urea, 5 parts of monoammonium phosphate, 2 parts of monopotassium phosphate, 2 parts of water-retaining and drought-resisting microbial agent and 4 parts of water-retaining agent.
The carrier is attapulgite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The Bacillus flexus is purchased from China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC1.15227; the bacillus subtilis is purchased from the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC1.14985.
The preparation method of the water-retention drought-resistant microbial agent comprises the following steps: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
The number of viable bacteria in the Bacillus flexus bacterial liquid is (1-1.5) multiplied by 10 10 cfu/mL; the viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 10g of carboxymethyl cellulose in 100mL of 10 mass percent sodium hydroxide solution, rapidly stirring at the rotating speed of 1000r/min for dissolving, adding 1g of urea and 1g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40min, then dropwise adding 5mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retention drought-resistant microbial powder: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 10g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring and dissolving at the rotating speed of 1000r/min, adding 1g of urea and 1g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40min, then dropwise adding 5mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
Example 2
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 45 parts of carrier, 43 parts of humic acid, 12 parts of charcoal, 6 parts of urea, 6 parts of monoammonium phosphate, 2 parts of monopotassium phosphate, 3 parts of water-retaining and drought-resisting microbial agent and 5 parts of water-retaining agent.
The carrier is diatomite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The Bacillus flexus is purchased from China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC1.15227; the bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) and has a preservation number of CGMCC1.14985.
The preparation method of the water-retention drought-resistant microbial agent comprises the following steps: respectively unfreezing the curvulus and the bacillus subtilis, then coating the unfrozen curvulus and the bacillus subtilis on a solid culture medium, and culturing for 20 hours at 28-30 ℃ to obtain activated curvulus strains and bacillus subtilis strains; and respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 36h to obtain a curvulus bacteria liquid and a bacillus subtilis bacteria liquid, carrying out spray drying on the bacteria liquid to obtain curvulus bacteria powder and bacillus subtilis powder, and mixing the two bacteria powders according to the mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
The number of viable bacteria in the Bacillus flexus bacterial liquid is (1-1.5) multiplied by 10 10 cfu/mL; the viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 12g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 2g of urea and 2g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40min, then dropwise adding 6mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retention drought-resistant microbial powder: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 20 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 36h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 12g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 2g of urea and 2g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40min, then dropwise adding 6mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
Example 3
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 45 parts of carrier, 45 parts of humic acid, 15 parts of biochar, 7 parts of urea, 6 parts of monoammonium phosphate, 2 parts of monopotassium phosphate, 4 parts of water-retaining and drought-resisting microbial agent and 5 parts of water-retaining agent.
The carrier is prepared by mixing attapulgite and diatomite according to a mass ratio of 1:1.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The Bacillus flexus is purchased from China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC1.15227; the bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) and has a preservation number of CGMCC1.14985.
The preparation method of the water-retention drought-resistant microbial agent comprises the following steps: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; and respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 48 hours to obtain a curvulus bacteria liquid and a bacillus subtilis bacteria liquid, carrying out spray drying on the bacteria liquid to obtain curvulus bacteria powder and bacillus subtilis powder, and mixing the two bacteria powders according to the mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
The number of viable bacteria in the Bacillus flexus bacterial liquid is (1-1.5) multiplied by 10 10 cfu/mL; the viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 13g of carboxymethyl cellulose in 100mL of 10 mass percent sodium hydroxide solution, rapidly stirring at the rotating speed of 2000r/min for dissolving, adding 3g of urea and 3g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 7mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retention drought-resistant microbial powder: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquids to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 13g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 3g of urea and 3g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 7mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, then sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to mix and granulate, obtaining a final product after granulating, fully drying, packaging and warehousing.
Example 4
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 48 parts of carrier, 45 parts of humic acid, 18 parts of charcoal, 8 parts of urea, 7 parts of monoammonium phosphate, 3 parts of monopotassium phosphate, 5 parts of water-retention and drought-resistant microbial agent and 7 parts of water-retaining agent.
The carrier is diatomite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The Bacillus flexus is purchased from China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC1.15227; the bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) and has a preservation number of CGMCC1.14985.
The preparation method of the water-retention drought-resistant microbial agent comprises the following steps: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
The number of viable bacteria in the bacillus flexus liquid is (1-1.5) multiplied by 10 10 cfu/mL; the viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 12g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 1g of urea and 3g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40min, then dropwise adding 8mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retaining drought-resisting microbial powder: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 12g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 1g of urea and 3g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40min, then dropwise adding 8mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
Example 5
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 50 parts of carrier, 50 parts of humic acid, 20 parts of charcoal, 10 parts of urea, 8 parts of monoammonium phosphate, 3 parts of monopotassium phosphate, 6 parts of water-retention and drought-resistant microbial agent and 8 parts of water-retention agent.
The carrier is diatomite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The Bacillus flexus is purchased from China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC1.15227; the bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) and has a preservation number of CGMCC1.14985.
The preparation method of the water-retention drought-resistant microbial agent comprises the following steps: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquids to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
The number of viable bacteria in the Bacillus flexus bacterial liquid is (1-1.5) multiplied by 10 10 cfu/mL; the number of viable bacteria in the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution by mass concentration, rapidly stirring at the rotating speed of 2000r/min for dissolving, adding 5g of urea and 5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retention drought-resistant microbial powder: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 48 hours to obtain a curvulus bacteria liquid and a bacillus subtilis bacteria liquid, carrying out spray drying on the bacteria liquid to obtain curvulus bacteria powder and bacillus subtilis powder, and mixing the two bacteria powders according to the mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution by mass concentration, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 5g of urea and 5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
Comparative example 1
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 50 parts of carrier, 50 parts of humic acid, 20 parts of charcoal, 10 parts of urea, 8 parts of monoammonium phosphate, 3 parts of monopotassium phosphate, 6 parts of microbial agent and 8 parts of water-retaining agent.
The carrier is diatomite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by fermenting bacillus curvatus with the preservation number of CGMCC1.15227.
The bacillus flexus is purchased from China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC1.15227.
The preparation method of the microbial agent comprises the following steps: unfreezing the curvularia, then coating the curvularia on a solid culture medium, and culturing for 24 hours at 28-30 ℃ to obtain an activated curvularia strain; inoculating the activated strain into a liquid amplification culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a curvating bacillus bacterial solution, and performing spray drying on the bacterial solution to obtain curvating bacillus bacterial powder, thus obtaining the microbial agent.
The number of viable bacteria in the Bacillus flexus bacterial liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution by mass concentration, rapidly stirring at the rotating speed of 2000r/min for dissolving, adding 5g of urea and 5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing microbial powder: unfreezing the curvularia, then coating the curvularia on a solid culture medium, and culturing for 24 hours at 28-30 ℃ to obtain an activated curvularia strain; inoculating the activated strain into a liquid amplification culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a curvating bacillus bacterial solution, and performing spray drying on the bacterial solution to obtain curvating bacillus bacterial powder to obtain a microbial agent;
(2) Preparing a water-retaining agent: dispersing 15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution by mass concentration, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 5g of urea and 5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
This comparative example was carried out in the same manner as example 5 except that only one strain of Bacillus curvatus was used as the microbial agent.
Comparative example 2
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 50 parts of carrier, 50 parts of humic acid, 20 parts of biochar, 10 parts of urea, 8 parts of monoammonium phosphate, 3 parts of monopotassium phosphate, 6 parts of microbial agent and 8 parts of water-retaining agent.
The carrier is diatomite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The microbial agent is bacterial powder obtained by fermenting bacillus subtilis with the preservation number of CGMCC1.14985.
The bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC1.14985.
The preparation method of the microbial agent comprises the following steps: unfreezing the bacillus subtilis, then coating the unfrozen bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain an activated bacillus subtilis strain; inoculating the activated strain into a liquid amplification culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a bacillus subtilis solution, and spray-drying the bacillus subtilis solution to obtain bacillus subtilis powder to obtain the microbial agent.
The viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, and the concentration of the extract is 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The preparation method of the water-retaining agent comprises the following steps: dispersing 15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution by mass concentration, rapidly stirring at the rotating speed of 2000r/min for dissolving, adding 5g of urea and 5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain the water-retaining agent.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing microbial powder: unfreezing the bacillus subtilis, then coating the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain an activated bacillus subtilis strain; inoculating the activated strain into a liquid amplification culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a bacillus subtilis solution, and spray-drying the bacillus subtilis solution to obtain bacillus subtilis powder to obtain a microbial agent;
(2) Preparing a water-retaining agent: dispersing 15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution by mass concentration, rapidly stirring at a rotating speed of 2000r/min for dissolving, adding 5g of urea and 5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 60min, then dropwise adding 10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to neutrality, drying in vacuum, and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
The comparative example is the same as example 5 except that only one species of Bacillus subtilis is used as the microbial agent.
Comparative example 3
A drought-resistant water-retention microbial agent is prepared from the following raw materials in parts by weight: 50 parts of carrier, 50 parts of humic acid, 20 parts of charcoal, 10 parts of urea, 8 parts of monoammonium phosphate, 3 parts of monopotassium phosphate, 6 parts of water-retention and drought-resistant microbial agent and 8 parts of water-retention agent.
The carrier is diatomite.
The biochar is obtained by carrying out anoxic high-temperature pyrolysis on plant straws.
The water-retaining drought-resisting microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
The bacillus flexus of the comparative example is purchased from China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC1.15227; the bacillus subtilis is purchased from China general microbiological culture Collection center (CGMCC) and has a preservation number of CGMCC1.14985.
The preparation method of the water-retention drought-resistant microbial agent comprises the following steps: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; and respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 48 hours to obtain a curvulus bacteria liquid and a bacillus subtilis bacteria liquid, carrying out spray drying on the bacteria liquid to obtain curvulus bacteria powder and bacillus subtilis powder, and mixing the two bacteria powders according to the mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
The number of viable bacteria in the bacillus flexus liquid is (1-1.5) multiplied by 10 10 cfu/mL; the number of viable bacteria in the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL；
The composition of the solid medium was: 1000mL of soybean meal and 300mL of inorganic salt solution, wherein the solution contains K 2 HPO 4 ·3H 2 O 2.0g/L，MgSO 4 ·7H 2 O 0.50g/L，pH8.0。
The composition of the liquid medium was: 15g of sodium chloride, 10g of yeast extract and 15g of tryptone, 1mol/L -1 Adjusting the pH value to 7.0-7.3 with NaOH, and adding ultrapure water to make the volume constant to 1L.
The water-retaining agent is polyacrylamide.
A preparation method of a drought-resistant water-retention microbial agent comprises the following preparation steps:
(1) Preparing water-retention drought-resistant microbial powder: unfreezing the curvularia bacillus and the bacillus subtilis respectively, then coating the unfrozen curvularia bacillus and the bacillus subtilis on a solid culture medium, and culturing for 24 hours at the temperature of 28-30 ℃ to obtain activated curvularia bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquids to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent;
(2) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
The comparative example is the same as example 5 except that the water-retaining agent is polyacrylamide as it is.
Planting test
And (3) test crops: capsicum and winter wheat
The experimental site: production base of Qingchun farmer professional cooperative society in orchid production area of Linyi city, shandong province
Determination of physiological indexes such as malondialdehyde and proline content
Tests were carried out using a potted plant growing method
The cultivation substrate is a mixture of test base garden soil, turfy soil and vermiculite, the proportion is 3. Each pot was planted with 5 pepper seedlings, each test group was 3 pots, and 3 replicates were used. And a blank control was set, i.e. no water-retaining agent was used.
And (5) placing the transplanted peppers in a rain shelter, quantitatively watering, and performing field management in the same way as the conventional field management. And (5) treating the hot pepper for 7 days after 30 days of planting, wherein the continuous moderate drought (the relative water content of the soil is 40-50%) is carried out on the hot pepper. And 8d, taking a fresh sample and returning the fresh sample to the laboratory for performance test.
The proline content is measured by an acid Pluronic method, and the malondialdehyde content is measured by a thiobarbituric acid method.
The test results are shown in table 1:
TABLE 1 influence of Water-retaining Agents on the plant physiological indices malondialdehyde and proline content
The data in the table show that the water-retaining agent can effectively reduce the malondialdehyde content of crops, increase the proline content,
can effectively improve the drought resistance of crops.
Wheat planting test
Designing a field experiment: in the field, a random block design is adopted, the length of a cell is 6m, the width of the cell is 6m, the interval of the cells is 0.5m, each treatment is repeated for 3 times, and the average value of the experimental results is obtained. Planting at 18 days 10 months in 2021 with a seeding rate of 150kg/hm 2 And the wheat line spacing is 20cm.
The fertilizing amount is as follows: 180kgN hm -2 、90kgP·hm -2 、90kgK·hm -2 And the fertilizer is applied as a base fertilizer at one time during soil preparation. All treatments adopt ground drip irrigation mode to irrigate overwintering water, extract water and grout water, 50mm each time.
Test groups: the water-retaining agents of examples 1 to 5 and the water-retaining agents of comparative examples 1 to 3 were applied together with a base fertilizer at an application rate of 5 kg/mu. And simultaneously setting a blank control CK, namely not using a water-retaining agent.
Index and method of measurement
Rainfall in test areas: the rainfall data of the test field is from a farmland climate monitoring system of the test field. Data is provided by the local agricultural weather department.
Considering the actual situation in the test area, the amount of groundwater up-moving supply, deep seepage and surface runoff are all ignored. The formula for calculating the water consumption of crops (ETa, mm) is:
ETa＝P+I+△W
in the formula: p is the precipitation (mm); i is the irrigation quantity (mm); Δ W is the amount of change (mm) in water storage capacity of the soil before and after the test.
Crop Water use efficiency (WUE, kg `hm) -2 `mm -1 ) The calculation formula of (2) is as follows:
WUE＝Y/ETa
wherein Y is the crop yield per unit area (kg. Hm) -2 )。
And (3) taking soil in the test area after harvesting for soil enzyme activity determination:
the phosphatase activity is measured by a nitrobenzene disodium phosphate (p-NPP) method, and the phosphatase activity is expressed as the amount of PNP generated after 1g +/-1 h; urease activity was determined by sodium phenolate-sodium hypochlorite colorimetry using NH generated after 1g + -24h 3 -N amount represents; the activity of the sucrase is measured by a 3,5-dinitrosalicylic acid colorimetric method, and is expressed by the amount of glucose generated after 24 hours and 72 hours by 1g of soil; the catalase activity was determined by potassium permanganate titration.
The planting test results and soil property test results are shown in tables 2-3:
TABLE 2 field test results
TABLE 3 results of soil enzyme activity test
As can be seen from data 2-3 in the table, the wheat yield, water consumption and water utilization rate, and the enzyme activity in the crop soil of the groups of examples 1-5 applying the water retention agent of the invention are superior to those of the comparative example, compared with the comparative example group, the addition of the microbial strains is reduced in the comparative examples 1-2, the synergistic effect among the strains disappears, and the comparative example 3 has no protection effect on the microorganisms, and the planting effect and the soil biological activity are reduced to different degrees. The selection of two strains and the protection effect of the water-retaining gel on microbial strains can be seen, the improvement of crop yield and the improvement of soil ecological activity are realized together, and the raw materials are matched with each other, so that the effect is weak.
It should be noted that the above-mentioned embodiments are only some of the preferred modes for implementing the invention, and not all of them. Obviously, all other embodiments obtained by persons of ordinary skill in the art based on the above-mentioned embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.
Claims (8)
1. The drought-resistant water-retention microbial agent is characterized by comprising the following raw materials in parts by weight: 40-50 parts of carrier, 40-50 parts of humic acid, 10-20 parts of charcoal, 5-10 parts of urea, 5-8 parts of monoammonium phosphate, 2-3 parts of monopotassium phosphate, 2-6 parts of water-retention and drought-resistant microbial agent and 4-8 parts of water-retaining agent.
2. The drought-resistant water-retaining microbial inoculant according to claim 1, wherein the carrier is attapulgite and/or diatomaceous earth.
3. The drought-resistant water-retaining microbial inoculant according to claim 1, wherein the biochar is obtained by anoxic and high-temperature pyrolysis of plant straws.
4. The drought-resistant water-retention microbial agent as claimed in claim 1, wherein the water-retention drought-resistant microbial agent is bacterial powder obtained by respectively fermenting bacillus curvatus with the preservation number of CGMCC1.15227 and bacillus subtilis with the preservation number of CGMCC1.14985 according to the mass ratio of 1:1 are mixed to obtain the product.
5. The drought-resistant water-retention microbial inoculant according to claim 4, wherein the preparation method of the water-retention drought-resistant microbial inoculant comprises the following steps: respectively unfreezing the curvularia and the bacillus subtilis, then coating the unfrozen curvularia and the bacillus subtilis on a solid culture medium, and culturing for 12-24h at 28-30 ℃ to obtain activated curvularia strain and bacillus subtilis strain; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24-48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain the water-retaining drought-resistant microbial agent.
6. The drought-resistant water-retaining microbial agent according to claim 5, wherein the viable count of the Bacillus curvatus bacterial liquid is (1-1.5) x 10 10 cfu/mL; the viable count of the bacillus subtilis liquid is (1-1.5) multiplied by 10 10 cfu/mL。
7. The drought-resistant water-retaining microbial inoculant according to claim 1, wherein the water-retaining agent is prepared by the following steps: dispersing 10-15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at the rotating speed of 1000-2000r/min for dissolving, adding 1-5g of urea and 1-5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40-60min, then dropwise adding 5-10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3-5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to be neutral, drying in vacuum, and crushing to obtain the water-retaining agent.
8. The preparation method of the drought-resistant water-retention microbial agent of any one of claims 1 to 7 is characterized by comprising the following preparation steps:
(1) Preparing water-retention drought-resistant microbial powder: unfreezing the curvating bacillus and the bacillus subtilis respectively, then coating the unfrozen curvating bacillus and the bacillus subtilis on a solid culture medium, and culturing for 12-24h at 28-30 ℃ to obtain activated curvating bacillus strains and bacillus subtilis strains; respectively inoculating the activated strains into a liquid expanded culture medium, culturing at 30 ℃ and 200rpm for 24-48h to obtain a curvating bacillus bacterial liquid and a bacillus subtilis bacterial liquid, performing spray drying on the bacterial liquid to obtain curvating bacillus bacterial powder and bacillus subtilis bacterial powder, and mixing the two bacterial powders according to a mass ratio of 1:1 to obtain a water-retaining drought-resistant microbial agent;
(2) Preparing a water-retaining agent: dispersing 10-15g of carboxymethyl cellulose in 100mL of 10% sodium hydroxide solution, rapidly stirring at the rotating speed of 1000-2000r/min for dissolving, adding 1-5g of urea and 1-5g of polyacrylamide, stirring and mixing at 0-5 ℃ for 40-60min, then dropwise adding 5-10mL of sodium chlorite, stirring for 30min, reacting at 60 ℃ for 3-5h after ultrasonic defoaming to obtain white viscous pasty gel, repeatedly washing with purified water to be neutral, and then drying in vacuum and crushing to obtain a water-retaining agent;
(3) Firstly, putting a carrier into a granulator, sequentially adding water-retaining drought-resistant microbial powder, a water-retaining agent, humic acid, biochar, urea, monoammonium phosphate and monopotassium phosphate, spraying water or a binder to carry out mixing granulation, obtaining a final product after granulation is finished, fully drying, packaging and warehousing.
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| CN117049912B (en) * | 2023-08-16 | 2024-04-19 | 德州市美丽宜居乡村建设服务中心(德州市农村改革与乡村振兴服务中心) | Organic microbial fertilizer for promoting wheat growth and preparation method thereof |
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Application publication date: 20230131 |