CN101757220A - Injection for treating piglet bacillary dysentery and preparation method thereof - Google Patents
Injection for treating piglet bacillary dysentery and preparation method thereof Download PDFInfo
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- CN101757220A CN101757220A CN200910309557A CN200910309557A CN101757220A CN 101757220 A CN101757220 A CN 101757220A CN 200910309557 A CN200910309557 A CN 200910309557A CN 200910309557 A CN200910309557 A CN 200910309557A CN 101757220 A CN101757220 A CN 101757220A
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Abstract
The invention discloses an injection for treating piglet bacillary dysentery and a preparation method thereof. 1000ml of injection comprises the following raw materials: 6-15g of Chinese anemone extractive, 5-10g of ash bark extractive, 5-15g of radix scutellariae extractive, 5-10g of amur corktree bark extractive and 10-20g of gentamycin sulfate. The invention mainly treats the piglet bacillary dysentery, has obvious curative effect particularly on piglet yellow-white dysentery, adopts an injection mode for drug administration, solves the difficulties of low drug bioavailability caused by incomplete piglet bioavailability function, and has the advantages of fast response, good curative effect, convenient use, no toxic or side effect, safety and reliability.
Description
Technical field
The present invention relates to a kind of animal-use drug, be specifically related to a kind of injection for the treatment of piglet bacillary dysentery and preparation method thereof.
Background technology
Because of the baby pig diarrhoea that bacterial infection causes very common, it comprises the baby swine paratyphoid that is caused by Salmonella infection, the piglet pujos blancos, the yellow scours that cause by coli-infection, infect the piglet red dysentery that causes by C type clostridieum welchii, with diarrhoea, fever and high mortality is cardinal symptom, and morbidity is more in 20 ages in days sees.Yellow and white dysentery of piglet is being collectively referred to as of yellow scour of piglet and piglet pujos blancos, and its M ﹠ M is all higher, is that to cause piglet to breed motility rate low, influences one of principal disease of pig industry benefit.Yellow scour of piglet claims the early onset colibacillosis again, mainly betides the piglet in a few hours to 7 age in days of birth back, primary disease especially with the sickness rate of the first-born pig that sow pig is farrowed for the highest, morbidity suddenly, mortality rate is also high.Piglet pujos blancos claims tardy property colibacillosis again, is caused by some serum of Escherichia coli, is a kind of acute infectious intestinal disease of 2-3 piglet in age in week, and sickness rate is higher, and case fatality rate is lower.
The yellow scour of piglet Clinical symptoms includes the curdled milk small pieces for drawing stench yellow pulpous state loose stool, slack anus, the anus loose stool of emerging during seizure.Morbidity piglet spirit is depressed, and inappetence dewaters rapidly, becomes thin, dies of exhaustion.Analyse common neck, subcutaneous abdomen edema, intestinal expands, and contains yellow pulpous state content of volume and gas, and intestinal mucosa is catarrhal inflammation to be changed, and the most serious with duodenum, jejunum, ileum take second place, and mesenteric lymph node has the little petechia of diffusivity.The piglet pujos blancos Clinical symptoms is that piglet is suffered from diarrhoea suddenly, feces is creamy white, canescence, faint yellow or yellow green, the paste sample has special stench flavor, and feces is rarer sometimes, have bubble, sometimes be mixed with the blood streak, the defecation increased frequency can reach for several times every day, defecation incontinence or proctoptosis when serious, vomiting sometimes.The morbidity piglet day by day becomes thin, and spirit is depressed, and inappetence is thick disorderly matt by hair, is afraid of cold, and accelerated breathing peels off and stays alone, or single lying prostrate in the pad grass, finally because of extremely weak dead.Analyse and be changed to that appearance is pale becomes thin, dehydration is serious, and intestinal wall is poor to follow the string, intestinal mucosa hyperemia, and hyporrhea, intestinal contents increases, and is water sample or foam sample.
Escherichia coli are very wide in distributed in nature, also often are present in the intestinal of pig, under normal circumstances can not cause morbidity.Bad when the feeding and management of piglet, the pig house health is bad, and is sombre moist, the weather cataclysm, and the milk of sow is crossed rare or overrich, when causing the piglet resistance to reduce, will cause a disease.The escherichia coli that eject in the sick pig body, its virulence strengthens, and when healthy piglet has eaten the food that sick swine excrement pollutes, just can cause morbidity.Therefore, 1 dysentery is arranged in the farrow, if untimely taking measures, will very fast propagation.Clinical treatment is used antibiotics always at present, because generation drug resistance and piglet gastrointestinal function are incomplete, therapeutic effect is unsatisfactory.
Summary of the invention
One of purpose of the present invention provides a kind of injection for the treatment of piglet bacillary dysentery.
Two of purpose of the present invention provides a kind of preparation method for the treatment of the injection of piglet bacillary dysentery.
The present invention is achieved through the following technical solutions:
A kind of injection for the treatment of piglet bacillary dysentery comprises following raw material in every 1000ml injection: Radix Pulsatillae extract 6～15g, Cortex Fraxini extract 5～10g, Radix Scutellariae extract 5～15g, Cortex Phellodendri extract 5～10g, gentamycin sulfate 10～20g.
Preferably: comprise following raw material in every 1000ml injection: Radix Pulsatillae extract 10g, Cortex Fraxini extract 7g, Radix Scutellariae extract 12g, Cortex Phellodendri extract 6g, gentamycin sulfate 14g.
Wherein also comprise adjuvant: ethanol, benzyl alcohol, tween 80 and sodium sulfite, consumption of ethanol is 80ml～250ml in every 1000ml injection, the consumption of benzyl alcohol is 50ml～200ml, and the consumption of tween 80 is 1ml～20ml, and the consumption of sodium sulfite is 0.5g～1.5g.
Raw materials used and the adjuvant of above-mentioned injection all is commercially available prod.
The present invention selects for use the effect of raw material to be summarized as follows:
Radix Pulsatillae extract: heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, antibiotic, cure mainly the poultry toxic-heat and blood stasis, damp and hot intestinal Huang, bacterial enteritis;
Cortex Fraxini extract: heat clearing and damp drying, astringent therapy makes eye bright, and is used for damp-heat diarrhea, and enteritis is had loose bowels;
Radix Scutellariae extract: have antibacterial, diuresis, antiinflammatory, resistance attitude and spasmolysis;
Cortex Phellodendri extract: heat clearing and damp drying, pathogenic fire purging remove to be steamed, and skin ulcer is treated in detoxifcation, have antibiotic, antiviral, the protozoacide effect is used for damp-heat dysentery;
Gentamycin sulfate: aminoglycosides broad ectrum antibiotic, multiple gram-negative bacteria and positive bacteria are all had antibacterial and bactericidal action, stronger to effects such as gram-negative bacteria such as bacillus pyocyaneus, aerobacteria, pneumobacillus, Salmonella, escherichia coli and Bacillus proteus and golden Portugal bacterium.
A kind of preparation method for the treatment of the injection of piglet bacillary dysentery, concrete steps are as follows:
(1) Radix Pulsatillae extract, Cortex Fraxini extract and Cortex Phellodendri extract mixing add distilled water 100ml～150ml, and ethanol 200ml～400ml stirred 4 ℃ of standing over night 2～4 hours;
(2) filtration step (1) gained mixed liquor, filtrate decompression is concentrated into half of original volume;
(3) tween 80, benzyl alcohol add in step (2) the gained concentrated solution, and 50 ℃～65 ℃ were stirred 2～5 hours down, make it abundant mixing, until clarification;
(4) gentamycin sulfate dissolved in distilled water adds in step (3) the gained mixed liquor;
(5) the Radix Scutellariae extract adding distil water is made into suspension, and the NaOH solution to wherein dripping 1mol/L dissolves fully until Radix Scutellariae extract, joins afterwards in step (4) the gained mixed liquor;
(6) sodium sulfite dissolved in distilled water adds in step (5) the gained mixed liquor;
(7) add active carbon in the mixed liquor of step (6) gained, the back that stirs was placed sucking filtration 30～60 minutes;
(8) distilled water is settled to 1000ml, adjust pH to 7～8;
(9) filter, packing, sterilization gets finished product.
The preparation method of the injection of above-mentioned treatment piglet bacillary dysentery, the Radix Pulsatillae extract consumption is 6～15g in wherein every 1000ml injection, the Cortex Fraxini extract consumption is 5～10g, the Radix Scutellariae extract consumption is 5～15g, the Cortex Phellodendri extract consumption is 5～10g, and the gentamycin sulfate consumption is 10～20g.
Preferably: the Radix Pulsatillae extract consumption is 10g in every 1000ml injection, and the Cortex Fraxini extract consumption is 7g, and the Radix Scutellariae extract consumption is 12g, and the Cortex Phellodendri extract consumption is 6g, and the gentamycin sulfate consumption is 14g.
The benzyl alcohol consumption is 50ml～200ml in every 1000ml injection, and the tween 80 consumption is 1ml～20ml, and the sodium sulfite consumption is 0.5g～1.5g.
The present invention has the following advantages:
(1) adopts the bonded prescription of Chinese medicine and western medicine, both solved and to have produced drug-fast problem, embodied the feasibility and the multiformity of Chinese prescription again, reach the effect for the treatment of both the principal and secondary aspects of a disease;
(2) determined curative effect, obvious to the piglet bacillary dysentery therapeutic effect, especially evident in efficacy at yellow and white dysentery of piglet;
(3) adopt the injecting method medication, solved the low difficult problem of medicine bioavailability that does not cause entirely owing to the piglet gastrointestinal function, instant effect, good effect, and also easy to use;
(4) the present invention is without any side effects, and is safe and reliable.
The specific embodiment
Below in conjunction with embodiment the present invention is elaborated.
Embodiment 1
Accurately take by weighing Radix Pulsatillae extract 6g, Cortex Fraxini extract 5g, Radix Scutellariae extract 5g, Cortex Phellodendri extract 5g, gentamycin sulfate 10g, ethanol 200ml, benzyl alcohol 50ml, tween 80 1ml, sodium sulfite 0.5g.
The preparation method of injection is as follows:
(1) Radix Pulsatillae extract that will take by weighing, Cortex Fraxini extract and Cortex Phellodendri extract mixing add distilled water 100ml, and ethanol 200ml stirred 4 ℃ of standing over night 2 hours;
(2) filtration step (1) gained mixed liquor, filtrate decompression is concentrated into half of original volume;
(3) tween 80, benzyl alcohol add in step (2) the gained concentrated solution, are heated to 50 ℃, stir 5 hours, make it abundant mixing, until clarification;
(4) gentamycin sulfate dissolved in distilled water adds in step (3) the gained mixed liquor;
(5) the Radix Scutellariae extract adding distil water is made into suspension, and the NaOH solution to wherein dripping 1mol/L dissolves fully until Radix Scutellariae extract, joins afterwards in step (4) the gained mixed liquor;
(6) sodium sulfite dissolved in distilled water adds in step (5) the gained mixed liquor;
(7) add the 2g active carbon in the mixed liquor of step (6) gained, the back that stirs was placed sucking filtration 30 minutes;
(8) distilled water is settled to 1000ml, adjust pH to 7;
(9) filter, packing, autoclaving is 30 minutes under 105 ℃, 0.5Mpa, finished product.
Embodiment 2
Accurately take by weighing Radix Pulsatillae extract 15g, Cortex Fraxini extract 10g, Radix Scutellariae extract 15g, Cortex Phellodendri extract 10g, gentamycin sulfate 20g, ethanol 400ml, benzyl alcohol 200ml, tween 80 20ml, sodium sulfite 1.5g.
The preparation method of injection is as follows:
(1) Radix Pulsatillae extract that will take by weighing, Cortex Fraxini extract and Cortex Phellodendri extract mixing add distilled water 150ml, and ethanol 400ml stirred 4 ℃ of standing over night 4 hours;
(2) filtration step (1) gained mixed liquor, filtrate decompression is concentrated into half of original volume;
(3) tween 80, benzyl alcohol add in step (2) the gained concentrated solution, are heated to 60 ℃, stir 3 hours, make it abundant mixing, until clarification;
(4) gentamycin sulfate dissolved in distilled water adds in step (3) the gained mixed liquor;
(5) the Radix Scutellariae extract adding distil water is made into suspension, and the NaOH solution to wherein dripping 1mol/L dissolves fully until Radix Scutellariae extract, joins afterwards in step (4) the gained mixed liquor;
(6) sodium sulfite dissolved in distilled water adds in step (5) the gained mixed liquor;
(7) add the 5g active carbon in the mixed liquor of step (6) gained, the back that stirs was placed sucking filtration 60 minutes;
(8) distilled water is settled to 1000ml, adjust pH to 8;
(9) filter, packing, autoclaving is 30 minutes under 105 ℃, 0.5Mpa, finished product.
Embodiment 3
Accurately take by weighing Radix Pulsatillae extract 10g, Cortex Fraxini extract 7g, Radix Scutellariae extract 12g, Cortex Phellodendri extract 6g, gentamycin sulfate 14g, ethanol 300ml, benzyl alcohol 120ml, tween 80 10ml, sodium sulfite 1g.
The preparation method of injection is as follows:
(1) Radix Pulsatillae extract that will take by weighing, Cortex Fraxini extract and Cortex Phellodendri extract mixing add distilled water 120ml, and ethanol 300ml stirred 4 ℃ of standing over night 3 hours;
(2) filtration step (1) gained mixed liquor, filtrate decompression is concentrated into half of original volume;
(3) tween 80, benzyl alcohol add in step (2) the gained concentrated solution, are heated to 65 ℃, stir 2 hours, make it abundant mixing, until clarification;
(4) gentamycin sulfate dissolved in distilled water adds in step (3) the gained mixed liquor;
(5) the Radix Scutellariae extract adding distil water is made into suspension, and the NaOH solution to wherein dripping 1mol/L dissolves fully until Radix Scutellariae extract, joins afterwards in step (4) the gained mixed liquor;
(6) sodium sulfite dissolved in distilled water adds in step (5) the gained mixed liquor;
(7) add the 3g active carbon in the mixed liquor of step (6) gained, the back that stirs was placed sucking filtration 60 minutes;
(8) distilled water is settled to 1000ml, adjust pH to 7.5;
(9) filter, packing, autoclaving is 30 minutes under 105 ℃, 0.5Mpa, finished product.
The medication of this medicine is intramuscular injection, and according to the piglet body weight, dose is 0.1ml/kg, and 2 times on the one, three is a course of treatment.
The injection that embodiment 1～embodiment 3 makes sees Table 1 to the extracorporeal bacteria inhibitor test measurement result of swine escherichia coli.
Table 1 injection of the present invention is to the extracorporeal bacteria inhibitor test measurement result of swine escherichia coli
(unit: mg/ml)
| Medicine | ??E.coli??1(MIC) | ??E.coli??2(MIC) | ??E.coli??3(MIC) | ??E.coli??4(MIC) | ??E.coli??5(MIC) | ??E.coli??6(MIC) | ?MIC 50 | ?MIC 90 |
| Embodiment 1 product | ??250 | ??62.5 | ??62.5 | ??62.5 | ??250 | ??500 | ?62.5 | ?250 |
| Embodiment 2 products | ??125 | ??31.5 | ??31.3 | ??31.3 | ??125 | ??125 | ?31.3 | ?125 |
| Embodiment 3 products | ??125 | ??31.5 | ??62.5 | ??31.3 | ??250 | ??125 | ?62.5 | ?125 |
Annotate: E.coli is the colibacillary abbreviation of English.
Claims (8)
1. an injection for the treatment of piglet bacillary dysentery is characterized in that, comprises following raw material in every 1000ml injection: Radix Pulsatillae extract 6～15g, Cortex Fraxini extract 5～10g, Radix Scutellariae extract 5～15g, Cortex Phellodendri extract 5～10g, gentamycin sulfate 10～20g.
2. the injection of treatment piglet bacillary dysentery according to claim 1 is characterized in that, preferably: comprise following raw material in every 1000ml injection: Radix Pulsatillae extract 10g, Cortex Fraxini extract 7g, Radix Scutellariae extract 12g, Cortex Phellodendri extract 6g, gentamycin sulfate 14g.
3. the injection of treatment piglet bacillary dysentery according to claim 1 and 2 is characterized in that, wherein also comprises adjuvant: ethanol, benzyl alcohol, tween 80 and sodium sulfite.
4. the injection of treatment piglet bacillary dysentery according to claim 3, it is characterized in that, consumption of ethanol is 80ml～250ml in wherein every 1000ml injection, the consumption of benzyl alcohol is 50ml～200ml, the consumption of tween 80 is 1ml～20ml, and the consumption of sodium sulfite is 0.5g～1.5g.
5. the preparation method of the injection of the described treatment piglet bacillary dysentery of claim 1 is characterized in that concrete steps are as follows:
(1) Radix Pulsatillae extract, Cortex Fraxini extract and Cortex Phellodendri extract mixing add distilled water 100ml～150ml, and ethanol 200ml～400ml stirred 4 ℃ of standing over night 2～4 hours;
(2) filtration step (1) gained mixed liquor, filtrate decompression is concentrated into half of original volume;
(3) tween 80, benzyl alcohol add in step (2) the gained concentrated solution, and 50 ℃～65 ℃ were stirred 2～5 hours down;
(4) gentamycin sulfate dissolved in distilled water adds in step (3) the gained mixed liquor;
(5) the Radix Scutellariae extract adding distil water is made into suspension, and the NaOH solution to wherein dripping 1mol/L dissolves fully until Radix Scutellariae extract, joins afterwards in step (4) the gained mixed liquor;
(6) sodium sulfite dissolved in distilled water adds in step (5) the gained mixed liquor;
(7) add active carbon in the mixed liquor of step (6) gained, the back that stirs was placed sucking filtration 30～60 minutes;
(8) distilled water is settled to 1000ml, adjust pH to 7～8;
(9) filter, packing, sterilization gets finished product.
6. the preparation method of the injection of treatment piglet bacillary dysentery according to claim 5, it is characterized in that, the Radix Pulsatillae extract consumption is 6～15g in every 1000ml injection, the Cortex Fraxini extract consumption is 5～10g, the Radix Scutellariae extract consumption is 5～15g, the Cortex Phellodendri extract consumption is 5～10g, and the gentamycin sulfate consumption is 10～20g.
7. the preparation method of the injection of treatment piglet bacillary dysentery according to claim 6, it is characterized in that, preferably: the Radix Pulsatillae extract consumption is 10g in every 1000ml injection, the Cortex Fraxini extract consumption is 7g, the Radix Scutellariae extract consumption is 12g, the Cortex Phellodendri extract consumption is 6g, and the gentamycin sulfate consumption is 14g.
8. the preparation method of the injection of treatment piglet bacillary dysentery according to claim 7, it is characterized in that, the consumption of benzyl alcohol is 50ml～200ml in every 1000ml injection, and the consumption of tween 80 is 1ml～20ml, and the consumption of sodium sulfite is 0.5g～1.5g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910309557A CN101757220A (en) | 2009-11-11 | 2009-11-11 | Injection for treating piglet bacillary dysentery and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910309557A CN101757220A (en) | 2009-11-11 | 2009-11-11 | Injection for treating piglet bacillary dysentery and preparation method thereof |
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| CN200910309557A Pending CN101757220A (en) | 2009-11-11 | 2009-11-11 | Injection for treating piglet bacillary dysentery and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102973702A (en) * | 2012-08-23 | 2013-03-20 | 郑州后羿制药有限公司 | Piglet yellow and white scour treatment compound traditional Chinese medicine composition and preparation method thereof |
| CN103263552A (en) * | 2013-05-09 | 2013-08-28 | 广西百通动物药业有限公司 | Pig gastrointestinal function improvement traditional Chinese medicine and preparation method thereof |
| CN103385928A (en) * | 2013-07-10 | 2013-11-13 | 吉林大学 | Compound medical preparation for curing diarrhea of young livestock and applications thereof |
-
2009
- 2009-11-11 CN CN200910309557A patent/CN101757220A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102973702A (en) * | 2012-08-23 | 2013-03-20 | 郑州后羿制药有限公司 | Piglet yellow and white scour treatment compound traditional Chinese medicine composition and preparation method thereof |
| CN102973702B (en) * | 2012-08-23 | 2014-12-17 | 郑州后羿制药有限公司 | Piglet yellow and white scour treatment compound traditional Chinese medicine composition and preparation method thereof |
| CN103263552A (en) * | 2013-05-09 | 2013-08-28 | 广西百通动物药业有限公司 | Pig gastrointestinal function improvement traditional Chinese medicine and preparation method thereof |
| CN103385928A (en) * | 2013-07-10 | 2013-11-13 | 吉林大学 | Compound medical preparation for curing diarrhea of young livestock and applications thereof |
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Application publication date: 20100630 |