CN101451132B - Complex enzyme preparation for China grass degumming as well as preparation method and use thereof - Google Patents
Complex enzyme preparation for China grass degumming as well as preparation method and use thereof Download PDFInfo
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- CN101451132B CN101451132B CN2007101146871A CN200710114687A CN101451132B CN 101451132 B CN101451132 B CN 101451132B CN 2007101146871 A CN2007101146871 A CN 2007101146871A CN 200710114687 A CN200710114687 A CN 200710114687A CN 101451132 B CN101451132 B CN 101451132B
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- grass degumming
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- 240000008564 Boehmeria nivea Species 0.000 title claims abstract description 57
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 28
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 239000000835 fiber Substances 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 27
- 150000001875 compounds Chemical class 0.000 claims abstract description 21
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 9
- 102100032487 Beta-mannosidase Human genes 0.000 claims abstract description 9
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 9
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 9
- 108010055059 beta-Mannosidase Proteins 0.000 claims abstract description 9
- 238000007670 refining Methods 0.000 claims abstract 3
- 239000007788 liquid Substances 0.000 claims description 14
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 6
- 239000012752 auxiliary agent Substances 0.000 claims description 4
- 239000004753 textile Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 206010044565 Tremor Diseases 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 abstract description 26
- 241000193755 Bacillus cereus Species 0.000 abstract description 9
- 108010002430 hemicellulase Proteins 0.000 abstract description 9
- 229940059442 hemicellulase Drugs 0.000 abstract description 7
- 108010059820 Polygalacturonase Proteins 0.000 abstract description 6
- 238000000855 fermentation Methods 0.000 abstract description 5
- 230000004151 fermentation Effects 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 5
- 238000011218 seed culture Methods 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 108010093305 exopolygalacturonase Proteins 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 4
- 238000013329 compounding Methods 0.000 abstract 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 abstract 1
- 229920001277 pectin Polymers 0.000 description 9
- 235000010987 pectin Nutrition 0.000 description 9
- 239000001814 pectin Substances 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 7
- 239000002131 composite material Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 4
- 238000012797 qualification Methods 0.000 description 4
- 108090000856 Lyases Proteins 0.000 description 3
- 102000004317 Lyases Human genes 0.000 description 3
- 229920002230 Pectic acid Polymers 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 239000000084 colloidal system Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000010318 polygalacturonic acid Substances 0.000 description 3
- 235000011121 sodium hydroxide Nutrition 0.000 description 3
- 241000218215 Urticaceae Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005554 pickling Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193375 Bacillus alcalophilus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 102100031149 Deoxyribonuclease gamma Human genes 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 241001134446 Niveas Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 108010031616 deoxyribonuclease gamma Proteins 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002879 macerating effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
- -1 zytase Proteins 0.000 description 1
Landscapes
- Chemical Or Physical Treatment Of Fibers (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a compound enzyme preparation for ramie degumming, and a preparation method thereof. The method comprises the steps of taking bacillus cereus obtained through separation as an original strain, preparing high-enzyme-activity alkaline pectinase which contains glucanase and neutral protease through seed culture and liquid-submerged fermentation, and compounding an appropriate amount of xylanase and mannanase on the basis of the alkaline pectinase so as to prepare the compound enzyme preparation. The invention also discloses a ramie degumming process, and the compound enzyme preparation is applied to an enzyme refining step in the ramie degumming process. The process enables the alkaline pectinase and hemicellulase to play a role jointly by compounding a plurality of biological enzymes, so as to achieve the aim of degumming. The process can save acid-base raw materials, and has the advantages of mild treatment conditions, low cost, good fiber quality, little pollution on environment and the like.
Description
Affiliated technical field
The present invention relates to a kind of China grass degumming and use compound enzymic preparation, especially also relate to the preparation method of said compound enzymic preparation and the application in china grass degumming process thereof.
Background technology
Ramie (Bochmeria nivea) belongs to Urticaceae (Urticaceae) perennial root, and per nnial herb originates in China, is called " China's grass " by European and American countries, and output accounts for 80% of the world.Through analyzing the ramie composition, its fibre content is more than 60%, and its phloem fiber is especially long and solid, and is carefully the longest in known bast-fibre, is a kind of important textile industry raw material.Yet being attached with a large amount of colloids on the ramee, accounting for gross weight 25-35%, mainly is pectin, semicellulose and xylogen etc., and according to the difference of ramee variety, its content can be up to 24-45%.Therefore to obtain industry and go up available fiber, the processing of must coming unstuck, and the come unstuck development and use of direct relation fiber of fiber.
Degumming method commonly used in the art is chemical caustic soda high pressure kiering method, and this method consumes a large amount of soda acids, along with acid-base raw materials appreciates significantly, causes cost to go up at double; Simultaneously, chemical Degumming produces a large amount of acidic and alkaline waste waters, can cause serious environmental to pollute.For this reason, many researchists are primarily focused on biological and biological chemical combined the coming unstuck.
Japanese Patent Laid is opened clear 51-149976 and is disclosed the method that a kind of mikrobe carries out China grass degumming, points out to contain cellulase in the mycetogenetic polygalacturonase, and acting condition is acid.Because of its degumming effect instability, and cause fibre strength to descend, be difficult in the industry use.Application number is that the Chinese invention patent application of CN85104284 discloses and utilizes bacillus to carry out the method for China grass degumming; One Chinese patent application CN97109044.0 discloses a strain bacillus alcalophilus and has been applied to China grass degumming, and Chinese patent ZL 01106844.2 discloses a strain Bacillus subtillis CCTCC M200038 and has been applied to China grass degumming.The disclosed content of above-mentioned each patent, all direct crude enzyme liquid with its said bacterial strain suitability for industrialized production is used for China grass degumming, does not take into account the situation of appended gelationus variety of ramee and pectinase activity height, on industrial application, has problems.
Summary of the invention
Technical problem to be solved by this invention provides a kind of compound enzymic preparation and preparation method thereof, application method, with processing that ramee is come unstuck, improves economic and social benefit.
China grass degumming according to the invention is used compound enzymic preparation, and its composition comprises:
Alkaline pectase is polygalacturonic acid lyase, LSD, neutral protease and hemicellulase.
Above-mentioned China grass degumming is used compound enzymic preparation, and said hemicellulase is zytase and mannase.
Above-mentioned China grass degumming is used compound enzymic preparation, in the compound enzymic preparation enzyme of each composition while still alive power be: alkaline pectase 300U/ml, LSD 4000U/ml, neutral protease 600U/ml, zytase 500U/ml, mannase 600U/ml.
Above-mentioned China grass degumming is used compound enzymic preparation; Said alkaline pectase obtains through following method: the bacillus cereus (Bacillus cereus) to be separated to is a starting strain, makes the alkaline pectase that high enzyme is lived through seed culture and liquid submerged fermentation.
Above-mentioned China grass degumming is applied to china grass degumming process with compound enzymic preparation, and said degumming technology comprises: with ramie prick, dress cage, enzyme whites silk, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, de-oiled water, tremble fiber crops, dry and obtain degummed ramie.
The described enzyme of above-mentioned china grass degumming process is practiced, and its main control parameters is:
Compound enzymic preparation addition 1-3%, textile auxiliary agent 0.2%-0.5% is and the ramie weight ratio; Ramie and come unglued liquid bath raio: 1: 12-1: 15, pH8.5-9.5, the temperature of coming unstuck is controlled at 40-50 ℃, and degummase liquid keeps static or dynamically, usually time is 2-10h.
Biological-chemical combination degumming method of the present invention can industrial application through the repeatedly test of different size.The bacillus cereus (Bacillus cereus) that obtains with separation is a starting strain, makes the alkaline pectin zymin that high enzyme is lived through seed culture and liquid submerged fermentation.Simultaneously, the enzyme component of preparation is carried out analysis revealed, except that the alkaline pectase that contains high vigor (referring to the polygalacturonic acid lyase), also contain LSD, neutral protease in the alkaline pectin zymin.The present invention is on the basis of original alkaline pectase, LSD and neutral protease, and more composite again hemicellulases are mainly zytase and mannase, can effectively remove the colloid in the ramie.
The present invention is composite through multiple enzyme, and its alkaline pectase and hemicellulase are played a role jointly, and the pectin in the hydrolysis ramie, semicellulose class material reach the purpose of coming unstuck.Advantages such as the method for the invention has cut the step pickling of one in the traditional method, has substituted the step soda boiling of one in the traditional technology, has saved a large amount of acid-base raw materials, has the treatment condition gentleness, and cost is low, and fiber quality is good, and environmental pollution is little.
After ramee uses compound enzymic preparation according to art breading according to the invention; The degummed ramie specific targets are: continuous three batches of average >=95% of degummed ramie qualification rate, residual gum content 2.54%, fibre strength 5.05gf/D, runner rate 7.26%, oil-containing 1.03%, fibre count 2073
It is salable product that ramie factory degummed ramie reaches following requirement, degummed ramie qualification rate >=90%, and residual gum content＜2.8%, fibre strength＞4.5gf/D, runner rate＜10%, oil-containing are between 0.6%～1.8%.
Embodiment
The starting material that the present invention relates to, the China grass degumming of having introduced except that this specification sheets is with the compound enzymic preparation, and all the other are the starting material of selling on the market.
Below in conjunction with specific embodiment the present invention is further specified.
Embodiment 1
The bacillus cereus (Bacillus cereus) that obtains with separation is a starting strain; Make the alkaline pectin zymin that high enzyme is lived through seed culture and liquid submerged fermentation; Composite again hemicellulase; Be mainly zytase and mannase, the enzyme that finally makes each composition in prozyme liquid power while still alive is: alkaline pectase 300U/ml, LSD 4000U/ml, neutral protease 600U/ml, zytase 500U/ml, mannase 600U/ml.Measure the vigor of polygalacturonase, zytase, mannase, an enzyme activity unit is defined as under condition determination, discharges the required enzyme amount of 1 μ mol galacturonic acid (or wood sugar or seminose) by the substrate PM.
The present invention carries out composite through multiple enzyme, alkaline pectase and hemicellulase are played a role jointly, is used for pectin, the semicellulose class material of hydrolysis ramie, to reach the purpose of coming unstuck.
Embodiment 2
China grass degumming of the present invention is used compound enzymic preparation, be applied to china grass degumming process, its technical process comprises:
With ramie prick, dress cage, enzyme whites silk, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, de-oiled water, tremble fiber crops, dry and obtain degummed ramie.
Above-mentioned technology is a kind of biological one chemical combined degumming technology, and center division technique step is consistent with traditional chemical Degumming method, describes with regard to its improvements below.
Described enzyme is practiced step:
To be that the compound enzymic preparation of 1-3%, the textile auxiliary agent of 0.2%-0.5% add come unglued liquid with the ramie weight ratio; The bath raio of ramie and come unglued liquid is: 1: 12-1: 15, and adjustment come unglued liquid pH value is 8.5-9.5, adds ramie and comes unstuck, and temperature is controlled at 40-50 ℃, and come unglued liquid keeps static or dynamically, usually time is 2-10h.
Described retted fibre step is:
After enzyme is practiced end, carry out retted fibre technology, concrete operations are: after the ramie enzyme is practiced completion, cover with plastic cloth, at 40 ℃ of left and right sides temperature held 12-24h.
Describedly wash numb step and be:
Completion is come unstuck the fiber crops of the system of macerating with 90 ℃ hot water sterilization 30min.
Described soda boiling step is:
The fiber crops of coming unstuck after the sterilization are added in the alkali lye, and NaOH concentration is 6-10g/L, and adds a certain amount of auxiliary agent according to the production needs, and addition is 0.2%-0.5% (in a ramie weight), and normal pressure boils and boils 2-4h.
Thereafter operation is undertaken by the traditional technology routine.
Through after the above-mentioned art breading, the degummed ramie specific targets are: continuous three batches of average >=95% of degummed ramie qualification rate, residual gum content 2.54%, fibre strength 5.05gf/D, runner rate 7.26%, oil-containing 1.03%, fibre count 2073.
It is salable product that ramie factory degummed ramie reaches following requirement, degummed ramie qualification rate >=90%, and residual gum content＜2.8%, fibre strength＞4.5gf/D, runner rate＜10%, oil-containing are between 0.6%～1.8%.
Biological-chemical combination degumming method of the present invention can industrial application through the repeatedly test of different size.The bacillus cereus (Bacillus cereus) that obtains with separation is a starting strain, makes the alkaline pectin zymin that high enzyme is lived through seed culture and liquid submerged fermentation.Simultaneously, the enzyme component of preparation is carried out analysis revealed, except that the alkaline pectase that contains high vigor (referring to the polygalacturonic acid lyase), also contain LSD, neutral protease in the alkaline pectin zymin.The present invention is on the basis of original alkaline pectase, LSD and neutral protease, and more composite again hemicellulases are mainly zytase and mannase, can effectively remove the colloid in the ramie.
The present invention is composite through multiple enzyme, and its alkaline pectase and hemicellulase are played a role jointly, and the pectin in the hydrolysis ramie, semicellulose class material reach the purpose of coming unstuck.Advantages such as the method for the invention has cut the step pickling of one in the traditional method, has substituted the step soda boiling of one in the traditional technology, has saved a large amount of acid-base raw materials, has the treatment condition gentleness, and cost is low, and fiber quality is good, and environmental pollution is little.
Claims (2)
1. a China grass degumming is used compound enzymic preparation, and it consists of:
Alkaline pectase 300U/ml, LSD 4000U/ml, neutral protease 600U/ml, zytase 500U/ml, mannase 600U/ml.
2. carry out the method for China grass degumming with the said China grass degumming of claim 1 with compound enzymic preparation, said method comprises:
With ramie prick, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, de-oiled water, tremble fiber crops, oven dry; Obtain degummed ramie; Described enzyme refining step; Its main control parameters is: compound enzymic preparation addition 1-3%, and textile auxiliary agent 0.2%-0.5% is and the ramie weight ratio; Ramie and come unglued liquid bath raio: 1: 12-1: 15, pH8.5-9.5, the temperature of coming unstuck is controlled at 40-50 ℃, and degummase liquid keeps static or dynamically, usually time is 2-10h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2007101146871A CN101451132B (en) | 2007-11-28 | 2007-11-28 | Complex enzyme preparation for China grass degumming as well as preparation method and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007101146871A CN101451132B (en) | 2007-11-28 | 2007-11-28 | Complex enzyme preparation for China grass degumming as well as preparation method and use thereof |
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| CN101451132A CN101451132A (en) | 2009-06-10 |
| CN101451132B true CN101451132B (en) | 2012-05-16 |
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Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684571B (en) * | 2009-08-19 | 2012-07-11 | 沅江浣溪沙酶技术有限公司 | Method for biologically degumming bast fibers |
| CN101787570B (en) * | 2009-08-19 | 2013-01-02 | 沅江浣溪沙酶技术有限公司 | Bast fiber pectase degumming method |
| CN102191566B (en) * | 2010-03-04 | 2015-11-25 | 上海康地恩生物科技有限公司 | The method of a kind of ramie fresh retting enzyme preparation and preparation method thereof and the fresh retting of ramie |
| CN102094249B (en) * | 2010-04-16 | 2015-02-04 | 汉寿鑫源纺织印染有限责任公司 | Method for degumming ramie by weakly alkaline liquid biological enzyme |
| CN102002467B (en) * | 2010-08-13 | 2012-11-07 | 东华大学 | Bacillus cereus DA3 strain and preparation method and application thereof |
| CN103774245B (en) * | 2014-02-25 | 2016-04-27 | 武汉纺织大学 | Ramie chain type continuous is without the useless point method for fiber that comes unstuck |
| CN104250858B (en) * | 2014-07-14 | 2016-06-08 | 东华大学 | A kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare bastose |
| CN105239167B (en) * | 2015-11-03 | 2018-05-22 | 宁波凯丽安科技股份有限公司 | A kind of high intensity is modified mulberry fibre |
| CN105239168B (en) * | 2015-11-03 | 2019-01-22 | 浙江品创知识产权服务有限公司 | A kind of mulberry fibre of antibacterial environment protection |
| CN106318927A (en) * | 2016-08-22 | 2017-01-11 | 安徽省华龙麻业有限公司 | Complex enzyme applied into ramie degumming, and preparation method thereof |
| CN106047842A (en) * | 2016-08-22 | 2016-10-26 | 安徽省华龙麻业有限公司 | Ramie degumming compound enzyme and preparation method thereof |
| CN106047841A (en) * | 2016-08-22 | 2016-10-26 | 安徽省华龙麻业有限公司 | Composite enzyme preparation for ramie boiling degumming and preparation method thereof |
| CN106222154A (en) * | 2016-08-22 | 2016-12-14 | 安徽省华龙麻业有限公司 | A kind of compound enzymic preparation improving China grass degumming efficiency and preparation method thereof |
| CN106047843B (en) * | 2016-08-22 | 2017-12-15 | 安徽省华龙麻业有限公司 | A kind of ramie boiling-off degumming complex enzyme formulation and preparation method thereof |
| CN106587553A (en) * | 2016-12-06 | 2017-04-26 | 钦州市高新技术产业服务中心 | Complex enzyme preparation and preparation method thereof |
| CN107268091B (en) * | 2017-06-30 | 2019-04-23 | 中国农业科学院麻类研究所 | A kind of bluish dogbane bast Degumming method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
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