CN106047843B - A kind of ramie boiling-off degumming complex enzyme formulation and preparation method thereof - Google Patents

A kind of ramie boiling-off degumming complex enzyme formulation and preparation method thereof Download PDF

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CN106047843B
CN106047843B CN201610702961.6A CN201610702961A CN106047843B CN 106047843 B CN106047843 B CN 106047843B CN 201610702961 A CN201610702961 A CN 201610702961A CN 106047843 B CN106047843 B CN 106047843B
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CN106047843A (en
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汪观清
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Jingde Hanhai Xingyun Intelligent Technology Research And Development Co ltd
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ANHUI HUALONG HEMP Co Ltd
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Abstract

A kind of ramie boiling-off degumming complex enzyme formulation and preparation method thereof, consisting of alpha amylase, alkaline pectase, beta glucan enzyme, neutral proteinase, zytase, mannonase papain, keratinase, sorbierite, 2 hydracrylic acids, sodium oxalate and bentonitic mixture, obtained enzyme preparation greatly improves ramie boiling-off degumming rate, and the time significantly shortens.

Description

A kind of ramie boiling-off degumming complex enzyme formulation and preparation method thereof
Technical field
The present invention relates to a kind of complex enzyme formulation and preparation method thereof, more particularly to a kind of ramie boiling-off degumming complex enzyme Preparation and preparation method thereof.
Background technology
Ramie (Bochmeria nivea) belongs to Urticaceae (Urticaceae) perennial root, and herbaceos perennial is former Is produced from China, is referred to as " China's grass " by European and American countries, yield accounts for the 80% of the world.By analyzing ramie composition, its fiber content More than 60%, its bast fiber is especially long and solid, most thin most long in known bast-fibre, is a kind of important weaving The raw material of industry.But substantial amounts of colloid is attached with ramee, account for gross weight 25-35%, mainly pectin, hemicellulose and Lignin etc., according to the difference of ramee variety, its content may be up to 24-45%.Therefore industrial available fiber is obtained, Degumming process, and the utilization of fiber degumming direct relation fiber must be carried out.
Conventional Degumming method is chemical caustic soda high pressure kiering method in the art, and this method consumes substantial amounts of soda acid, As acid-base raw materials significantly appreciate, cost is caused to go up at double；Meanwhile chemical Degumming produces substantial amounts of acidic and alkaline waste water, can make Into serious environmental pollution.Therefore, many researchers are primarily focused on biology and a biological chemical combined degumming.
Japanese Patent Laid-Open No. Sho 51-149976 discloses a kind of method that microorganism carries out China grass degumming, it is indicated that fungi Contain cellulase in caused pectase, the condition to work is acidity.Because its degumming effect is unstable, and cause fiber strong Degree declines, and is industrially difficult to apply.Application No. CN85104284 Chinese invention patent application, which discloses, utilizes brood cell's bar The method that bacterium carries out China grass degumming, Chinese patent application CN97109044.0 disclose one plant of bacillus alcalophilus and are applied to China grass degumming, Chinese patent ZL 01106844.2 disclose one plant of Bacillus subtillis CCTCC M200038 and are applied to China grass degumming.Above-mentioned each patent disclosure of that, is directly used for ramie by the crude enzyme liquid of its bacterial strain industrialized production Degumming, a kind of 2007101146871 China grass degumming of Chinese patent disclose a kind of enzyme preparation with complex enzyme formulation and taken off for ramie Glue, but the situation of glue qualitative diversity and pectinase activity height appended by ramee is not taken into account, deposited in commercial Application In problems.
The content of the invention
The present invention provides a kind of complex enzyme formulation, using papain enhance alkaline pectase, dextranase, in The hydrolysis to pectin of property protease, zytase, mannase, makes it be converted into small differentiation compound；Alpha-amylase can The hydrolysis of papain is improved, causes neutral pectin molecule solvation degree to greatly improve, bentonite provides load for enzyme preparation Body, while pectin and its catabolite are adsorbed when enzyme preparation plays a role, sorbierite is advantageous to improve the use effect of keratin Rate, 2 hydroxy propanoic acid, sodium oxalate are advantageous to improve bentonitic adsorption efficiency, and complex enzyme formulation is made.
A kind of ramie boiling-off degumming complex enzyme formulation, consisting of alpha-amylase, alkaline pectase, beta glucan Enzyme, neutral proteinase, zytase, mannonase papain, keratinase, sorbierite, 2 hydroxy propanoic acid, oxalic acid Sodium and bentonitic mixture.
Present invention also offers the ramie boiling-off degumming complex enzyme formulation preparation method, by the parts by weight of alpha-amylase 5, alkali Property pectase 15 parts by weight, the parts by weight of 1,4 beta-glucanase 45, the parts by weight of neutral proteinase 12 ~ 14, the weight of zytase 16 ~ 18 Measure part, the parts by weight of mannase 22, the parts by weight of papain 22, the parts by weight of keratinase 32, the weight of sorbierite 0.1 ~ 0.5 Amount part, the parts by weight of 2 hydroxy propanoic acid 0.3 ~ 0.5, the parts by weight of sodium oxalate 0.3 and bentonite are well mixed at normal temperatures for 16 ~ 31%, It is heated to 35 DEG C to stir, mixing time 1h, obtains complex enzyme formulation.
Its preferable preparation method is by the parts by weight of alpha-amylase 5, the parts by weight of alkaline pectase 15,1,4 beta-glucanase 45 Parts by weight, the parts by weight of neutral proteinase 12 ~ 13, the parts by weight of zytase 16 ~ 17, the parts by weight of mannase 22, pawpaw egg The white parts by weight of enzyme 22, the parts by weight of keratinase 32, the parts by weight of sorbierite 0.1 ~ 0.4, the parts by weight of 2 hydroxy propanoic acid 0.3 ~ 0.4, grass The sour parts by weight of sodium 0.3 and bentonite are well mixed at normal temperatures for 21 ~ 31%, are heated to 35 DEG C and are stirred, mixing time 1h, Obtain complex enzyme formulation.
By ramie binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, de-oiled water, tremble it is numb, dry, Degummed ramie, described enzyme refining step are obtained, its main control parameters is：Complex enzyme formulation addition 1-3%, body lotion ratio are 1： 15, pH8.5-9.5, it is 1% and bentonite 2% to add sodium oxalate, and at 40-50 DEG C, degumming enzyme liquid keeps stirring degumming temperature control Mix state, usually time 1-2h.
Beneficial effects of the present invention:
（1）Traditional papain is used for the degraded of albumen, and the present invention enhances alkalescence with a small amount of papain The hydrolysis to pectin of pectase, dextranase, neutral proteinase, zytase, mannase, makes it be converted into small point Compound；
（2）Alpha-amylase can improve the hydrolysis of papain, cause neutral pectin molecule solvation degree to carry significantly Height, and improve its hydrolysis to hemicellulose；
（3）Papain used in the present invention comes from the boat chemical products Co., Ltd of Henan hundred million, and alpha-amylase is Pei founds bioengineering（Shanghai）Co., Ltd, alkaline pectase produce for Shanghai KDN Biotech Co. Ltd., and β-Portugal gathers Carbohydrase is sold by Shanghai Ji Ning Industrial Co., Ltd.s, and keratinase is Jinan Nuo Neng bioengineering Co., Ltd, neutral protein Enzyme, zytase, mannase are that any main component and any producer of vigor are commercially available；
（4）Enzyme activity selected by the present invention is as follows successively:Alpha-amylase 7000U/ml, alkaline pectase 300U/ml, 1,4 beta-glucanase 4000U/ml, neutral proteinase 600U/ml, zytase 500U/ml, mannase 600U/ml, wood Melon protease 50000U/ml and keratinase 100000U/ml
（5）Bentonite provides carrier for enzyme preparation, while pectin and its catabolite are adsorbed when enzyme preparation plays a role, Sorbierite is advantageous to improve the service efficiency of keratin, and 2 hydroxy propanoic acid, sodium oxalate are advantageous to improve bentonitic adsorption efficiency.
（6）The enzyme preparation compares traditional enzyme preparation, and degumming efficiency greatly improves.
The method of the invention has cut the step pickling in conventional method, instead of the step soda boiling in traditional handicraft, Substantial amounts of acid-base raw materials are saved, there is the advantages that treatment conditions are gentle, and cost is low, and fiber quality is good, and environmental pollution is small, phase It is more efficient than traditional enzyme preparation.
Embodiment
With reference to embodiment, the present invention is described in further detail, but does not form any limit to the present invention System.
A kind of ramie boiling-off degumming complex enzyme formulation, consisting of alpha-amylase, alkaline pectase, 1,4 beta-glucanase, Neutral proteinase, zytase, mannonase papain, keratinase, sorbierite, 2 hydroxy propanoic acid, sodium oxalate and Bentonitic mixture.
Present invention also offers the ramie boiling-off degumming complex enzyme formulation preparation method, by the parts by weight of alpha-amylase 5, alkali Property pectase 15 parts by weight, the parts by weight of 1,4 beta-glucanase 45, the parts by weight of neutral proteinase 12 ~ 14, the weight of zytase 16 ~ 18 Measure part, the parts by weight of mannase 22, the parts by weight of papain 22, the parts by weight of keratinase 32, the weight of sorbierite 0.1 ~ 0.5 Amount part, the parts by weight of 2 hydroxy propanoic acid 0.3 ~ 0.5, the parts by weight of sodium oxalate 0.3 and bentonite are well mixed at normal temperatures for 16 ~ 31%, It is heated to 35 DEG C to stir, mixing time 1h, obtains complex enzyme formulation.
Further, priority scheme is by the parts by weight of alpha-amylase 5, the parts by weight of alkaline pectase 15,1,4 beta-glucanase 45 parts by weight, the parts by weight of neutral proteinase 12 ~ 13, the parts by weight of zytase 16 ~ 17, the parts by weight of mannase 22, pawpaw The parts by weight of protease 22, the parts by weight of keratinase 32, the parts by weight of sorbierite 0.1 ~ 0.4, the parts by weight of 2 hydroxy propanoic acid 0.3 ~ 0.4, The parts by weight of sodium oxalate 0.3 and bentonite are well mixed at normal temperatures for 21 ~ 31%, are heated to 35 DEG C and are stirred, mixing time 1h, obtain complex enzyme formulation.
By ramie binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, de-oiled water, tremble it is numb, dry, Degummed ramie, described enzyme refining step are obtained, its main control parameters is：Complex enzyme formulation addition 1-3%, body lotion ratio are 1： 15, pH8.5-9.5, it is 1% and bentonite 2% to add sodium oxalate, and at 40-50 DEG C, degumming enzyme liquid keeps stirring degumming temperature control Mix state, usually time 1-2h.
Example 1
By alpha-amylase 5kg, alkaline pectase 15kg, 1,4 beta-glucanase 45kg, neutral proteinase 12kg, xylan Enzyme 16kg, mannase 22kg, papain 22kg, keratinase 32kg, sorbierite 0.1kg, 2 hydroxy propanoic acid 0.3kg, sodium oxalate 0.3kg and bentonite are that 21kg is well mixed at normal temperatures, are heated to 35 DEG C and stir, mixing time 1h, obtain complex enzyme formulation.
Using this example according to by ramie binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, take off Profit, fiber crops, drying are trembled, obtain degummed ramie, described enzyme refining step, its main control parameters is：Complex enzyme formulation addition 1- 3%, body lotion ratio is 1：15, pH8.5-9.5, it is 1% and bentonite 2% to add sodium oxalate, and degumming temperature control is at 40 DEG C, degumming Enzyme liquid is kept stirring for state, usually time 1h.Measurement data finds ramee using complex enzyme formulation according to institute of the present invention After stating PROCESS FOR TREATMENT, the data measured are shown in Table 1.
The present example 1 of table 1 is used for ramee test data
Continuous three criticize flat of qualification rate/% Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 99 1.54 5.05 7.26 0.76 2073
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
Example 2
By alpha-amylase 5kg, alkaline pectase 15kg, 1,4 beta-glucanase 45kg, neutral proteinase 13kg, xylan Enzyme 17kg, mannase 22kg, papain 22kg, keratinase 32kg, sorbierite 0.4kg, 2 hydroxy propanoic acid 0.4kg, sodium oxalate 0.3kg and bentonite 31kg are well mixed at normal temperatures, are heated to 35 DEG C and are stirred, mixing time 1h, Obtain complex enzyme formulation.
Using this example according to by ramie binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, take off Profit, fiber crops, drying are trembled, obtain degummed ramie, described enzyme refining step, its main control parameters is：Complex enzyme formulation addition 1- 3%, body lotion ratio is 1：15, pH8.5-9.5, it is 1% and bentonite 2% to add sodium oxalate, and degumming temperature control is at 40 DEG C, degumming Enzyme liquid is kept stirring for state, usually time 1h.Measurement data finds ramee using complex enzyme formulation according to institute of the present invention After stating PROCESS FOR TREATMENT, the data measured are shown in Table 2.
The present example 2 of table 2 is used for ramee test data
Continuous three criticize flat of qualification rate/% Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 99 0.54 5.55 4.26 0.66 2073
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
Example 3
Alpha-amylase 5kg, alkaline pectase 15kg, 1,4 beta-glucanase 45kg, neutral proteinase 12.5kg, wood is poly- Carbohydrase 16.5kg, mannase 22kg, papain 22kg, keratinase 32kg, sorbierite 0.25kg, 2- hydroxyl third Sour 0.35kg, sodium oxalate 0.3kg and bentonite 26kg are well mixed at normal temperatures, are heated to 35 DEG C and are stirred, mixing time 1h, obtain complex enzyme formulation.
Using this example according to by ramie binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, take off Profit, fiber crops, drying are trembled, obtain degummed ramie, described enzyme refining step, its main control parameters is：Complex enzyme formulation addition 1- 3%, body lotion ratio is 1：15, pH8.5-9.5, it is 1% and bentonite 2% to add sodium oxalate, and degumming temperature control is at 40 DEG C, degumming Enzyme liquid is kept stirring for state, usually time 1h.Measurement data finds ramee using complex enzyme formulation according to institute of the present invention After stating PROCESS FOR TREATMENT, the data measured are shown in Table 3.
The present example 3 of table 3 is used for ramee test data
Continuous three criticize flat of qualification rate/% Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 98 0.14 6.55 3.26 0.66 2079
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
The above described is only a preferred embodiment of the present invention, being not the limitation for making other forms to the present invention, appoint What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc. Imitate embodiment.But it is every without departing from technical solution of the present invention content, the technical spirit according to the present invention is to above example institute Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.

Claims (3)

  1. A kind of 1. ramie boiling-off degumming complex enzyme formulation, it is characterised in that:Consisting of the parts by weight of alpha-amylase 5, alkaline fruit The parts by weight of glue enzyme 15, the parts by weight of 1,4 beta-glucanase 45, neutral proteinase 12~14 parts by weight, zytase 16~18 parts by weight, The parts by weight of mannase 22, the parts by weight of papain 22, the parts by weight of keratinase 32, the .1 of sorbierite 0~0 .5 weight Part, the .3 of 2 hydroxy propanoic acid 0~0 .5 parts by weight, the .3 parts by weight of sodium oxalate 0 and the parts by weight of bentonite 21 ~ 31.
  2. A kind of 2. preparation method of ramie boiling-off degumming complex enzyme formulation as claimed in claim 1, it is characterised in that：By α- The parts by weight of amylase 5, the parts by weight of alkaline pectase 15, the parts by weight of 1,4 beta-glucanase 45, neutral proteinase 12~14 parts by weight, Zytase 16~18 parts by weight, the parts by weight of mannase 22, the parts by weight of papain 22, the parts by weight of keratinase 32, The .1 of sorbierite 0~0 .5 parts by weight, the .3 of 2 hydroxy propanoic acid 0~0 .5 parts by weight, the .3 parts by weight of sodium oxalate 0 and bentonite 21 ~ 31 Parts by weight are well mixed at normal temperatures, are heated to 35 DEG C and are stirred, mixing time 1h, obtain complex enzyme formulation.
  3. A kind of 3. preparation method of ramie boiling-off degumming complex enzyme formulation according to claim 2, it is characterised in that：Will The parts by weight of alpha-amylase 5, the parts by weight of alkaline pectase 15, the parts by weight of 1,4 beta-glucanase 45, neutral proteinase 12~13 weight Part, zytase 16~17 parts by weight, the parts by weight of mannase 22, the parts by weight of papain 22, the weight of keratinase 32 Part, the .1 of sorbierite 0~0 .4 parts by weight, the .3 of 2 hydroxy propanoic acid 0~0 .4 parts by weight, the .3 parts by weight of sodium oxalate 0 and bentonite are 21 ~ 31 parts by weight are well mixed at normal temperatures, are heated to 35 DEG C and are stirred, mixing time 1h, obtain complex enzyme formulation.
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