CN106047842A - Ramie degumming compound enzyme and preparation method thereof - Google Patents

Ramie degumming compound enzyme and preparation method thereof Download PDF

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Publication number
CN106047842A
CN106047842A CN201610702956.5A CN201610702956A CN106047842A CN 106047842 A CN106047842 A CN 106047842A CN 201610702956 A CN201610702956 A CN 201610702956A CN 106047842 A CN106047842 A CN 106047842A
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parts
enzyme
compound enzyme
beta
amylase
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汪观清
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ANHUI HUALONG HEMP Co Ltd
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ANHUI HUALONG HEMP Co Ltd
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
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    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
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    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
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Abstract

The invention discloses a ramie degumming compound enzyme, which comprises the following raw materials by weight: 2-5 parts of beta-glucosidase, 2-5 parts of alpha-amylase, 1-3 parts of trypsin, 12-18 parts of alkaline pectinase, 30-36 parts of beta-glucanase, 10-15 parts of neutral protease, 15-18 parts of xylanase, 15-26 parts of mannanase, 15-22 parts of bromelain, 0.1-0.4 part of erythritol, 0.1-0.4 part of 2-hydroxypropionic acid, 0.1-0.5 part of sodium citrate, 0.2-0.8 part of potassium sorbate, and 28-40 parts of attapulgite clay. The invention further discloses a preparation method of the ramie degumming compound enzyme. According to the present invention, with the application of the ramie degumming compound enzyme in the ramie degumming, the effects of stable degumming effect, fiber injury reducing and fiber strength increasing can be provided, and the wide range industrial application can be achieved.

Description

China grass degumming compound enzyme and preparation method
Technical field
The present invention relates to a kind of China grass degumming compound enzyme and preparation method, belong to textile applications field.
Background technology
Boehmeria (Bochmeria nivea) genus Urticaceae (Urticaceae) perennial root, herbaceos perennial, former Producing China, be referred to as " China's grass " by European and American countries, yield accounts for the 80% of the world.By analyzing Boehmeria composition, its fiber content More than 60%, its phloem fiber is the longest and solid, the longest in known bast-fibre, is a kind of important weaving The raw material of industry.But be attached with substantial amounts of colloid on ramee, account for gross weight 25-35%, mainly pectin, hemicellulose and Lignins etc., according to the difference of ramee variety, its content may be up to 24-45%.Therefore industrial available fiber is obtained, Must carry out degumming process, and the exploitation of fiber degumming direct relation fiber.
Degumming method conventional in the art is chemistry Caustic soda high pressure kiering method, and the method consumes substantial amounts of soda acid,
Along with acid-base raw materials significantly appreciates, cost is caused to go up at double；Meanwhile, chemical Degumming produces substantial amounts of acidic and alkaline waste water, Serious environmental pollution can be caused.To this end, many research worker are primarily focused on a biological and biological chemical combined degumming On.
Japanese Patent Laid-Open No. Sho 51-149976 discloses a kind of method that microorganism carries out China grass degumming, it is indicated that fungus Containing cellulase in the pectase produced, the condition worked is acidity.Because its degumming effect is unstable, and cause fiber strong Degree declines, and industrial being difficult to is applied.The Chinese invention patent application of Application No. CN85104284 discloses and utilizes brood cell's bar Bacterium carries out the method for China grass degumming, and Chinese patent application CN97109044.0 discloses a strain bacillus alcalophilus and is applied to China grass degumming, Chinese patent ZL 01106844.2 discloses a strain Bacillus subtillis CCTCC M200038 and is applied to China grass degumming.Above-mentioned each patent disclosure of that, is all directly used for Boehmeria by the crude enzyme liquid of its described bacterial strain industrialized production Degumming, 2,007,101,146,871 1 kinds of China grass degumming compound enzymes of Chinese patent disclose a kind of enzyme preparation for China grass degumming, But do not take into account glue qualitative diversity appended by ramee and the situation of pectinase activity height, commercial Application exists all Many problems.
Summary of the invention
For overcoming above-mentioned deficiency, it is an object of the present invention to provide a kind of China grass degumming compound enzyme, use this compound enzyme to apply In China grass degumming, degumming effect can be played and stablize, reduce fibre damage, the effect of raising fibre strength, and can carry out big Range of industrialization is applied.
The solution of the present invention is as follows: a kind of China grass degumming compound enzyme, is composed of the following raw materials by weight: β-glucose Glycosides enzyme 2-5 part, α-amylase 2-5 part, trypsin 1-3 part, alkaline pectase 12-18 part, 1,4 beta-glucanase 30-36 part, in Property protease 10-15 part, xylanase 15-18 part, mannase 15-26 part, bromelain 15-22 part, erythritol 0.1- 0.4 part, 2 hydroxy propanoic acid 0.1-0.4 part, sodium citrate 0.1-0.5 part, potassium sorbate 0.2-0.8 part and attapulgite clay 28- 40 parts.
Further, be composed of the following raw materials by weight: beta-glucosidase 3 parts, α-amylase 4 parts, trypsin 2 parts, Alkaline pectase 16 parts, 1,4 beta-glucanase 32 parts, neutral protease 12 parts, xylanase 16 parts, mannase 19 parts, spinach Trailing plants enzyme 18 parts, erythritol 0.3 part, 2 hydroxy propanoic acid 0.2 part, sodium citrate 0.3 part, potassium sorbate 0.5 part and attapulgite are viscous 31 parts of soil.
Further, be composed of the following raw materials by weight: beta-glucosidase 2 parts, α-amylase 2 parts, trypsin 3 parts, Alkaline pectase 15 parts, 1,4 beta-glucanase 33 parts, neutral protease 11 parts, xylanase 17 parts, mannase 15 parts, spinach Trailing plants enzyme 22 parts, erythritol 0.4 part, 2 hydroxy propanoic acid 0.1 part, sodium citrate 0.14 part, potassium sorbate 0.3 part and attapulgite are viscous 38 parts of soil.
Further, be composed of the following raw materials by weight: beta-glucosidase 4 parts, α-amylase 3 parts, trypsin 1 part, Alkaline pectase 12 parts, 1,4 beta-glucanase 30 parts, neutral protease 14 parts, xylanase 15 parts, mannase 22 parts, spinach Trailing plants enzyme 16 parts, erythritol 0.2 part, 2 hydroxy propanoic acid 0.3 part, sodium citrate 0.35 part, potassium sorbate 0.6 part and attapulgite are viscous 29 parts of soil.
The preparation method of China grass degumming compound enzyme, by each raw material mix homogeneously at normal temperatures, is heated to 35-40 DEG C of stirring Uniformly, mixing time 1-1.5h, i.e. arrives compound enzyme.
Enzyme activity selected by the present invention is as follows: beta-glucosidase 5000U/ml, trypsin 4000U/ml, α-amylase 6000U/ml, alkaline pectase 300U/ml, 1,4 beta-glucanase 4000U/ml, neutral protease 600U/ml, wood Dextranase 500U/ml, mannase 600U/ml, bromelain 50000U/ml.
Present invention bromelain enhances alkaline pectase, glucanase, neutral protease, xylanase, mannan The hydrolysis to pectin of enzyme so that it is be converted into little differentiation compound；α-amylase can improve the hydrolysis of bromelain, causes neutral fruit Xanthan molecule solvation degree is greatly improved, and improves its hydrolysis to hemicellulose.
Present invention attapulgite clay provides carrier for enzyme preparation, adsorb when enzyme preparation plays a role simultaneously pectin and Catabolite, erythritol is conducive to improving the service efficiency of Collagenase, 2 hydroxy propanoic acid, sodium citrate, potassium sorbate Be conducive to improving the adsorption efficiency of attapulgite clay.Comparing tradition enzyme preparation, degumming efficiency is greatly improved.
The method of the invention has cut the step pickling in traditional method, instead of the step soda boiling in traditional handicraft, Saving substantial amounts of acid-base raw materials, have treatment conditions gentle, low cost, fiber quality is good, the advantages such as environmental pollution is little, phase The traditional enzyme preparation of ratio, in hgher efficiency.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Beta-glucosidase used in the present invention, α-amylase, 1,4 beta-glucanase, neutral protease, xylanase, pancreas Protease is produced by Shi Feng bio tech ltd, Shanghai, and alkaline pectase is that Shanghai KDN Biotech Co. Ltd. is raw Produce, mannase be any main component and any producer of vigor the most commercially available；Bromelain is by Dong Henghua road, Nanning biology section Skill Co., Ltd produces.
Embodiment 1
By beta-glucosidase 3 grams, α-amylase 4 grams, trypsin 2 grams, alkaline pectase 16 grams, 1,4 beta-glucanase 32 grams, Neutral protease 12 grams, xylanase 16 grams, mannase 19 grams, bromelain 18 grams, erythritol 0.3 gram, 2-hydroxyl third Acid 0.2 gram, sodium citrate 0.3 gram, potassium sorbate 0.5 gram and attapulgite clay 31 grams, mix homogeneously at normal temperatures, be heated to 35 DEG C stir, mixing time 1h, obtain compound enzyme.
Apply this example according to by Boehmeria binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, removing oil Water, trembling fiber crops, drying, obtain degummed ramie, described enzyme refining step, its main control parameters is: compound enzyme addition 1-3%, bath Liquor ratio is 1:15, pH8.5-9.5, and degumming temperature controls at 40 DEG C, and degummase liquid is kept stirring for state, and usually time is 1h. Measuring ramee uses compound enzyme according to data after PROCESS FOR TREATMENT of the present invention.
Table 1 present example 1 tests data for ramee
The continuous three crowdes of average/% of qualification rate Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 99 1.58 5.27 7.36 0.785 2241
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
Embodiment 2
By beta-glucosidase 2 grams, α-amylase 2 grams, trypsin 3 grams, alkaline pectase 15 grams, 1,4 beta-glucanase 33 grams, Neutral protease 11 grams, xylanase 17 grams, mannase 15 grams, bromelain 22 grams, erythritol 0.4 gram, 2-hydroxyl third Acid 0.1 gram, sodium citrate 0.14 gram, potassium sorbate 0.3 gram and attapulgite clay 38 grams mix homogeneously at normal temperatures, be heated to 38 DEG C stir, mixing time 1.2h, i.e. arrives compound enzyme.
Apply this example according to by Boehmeria binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, removing oil Water, trembling fiber crops, drying, obtain degummed ramie, described enzyme refining step, its main control parameters is: compound enzyme addition 1-3%, bath Liquor ratio is 1:15, pH8.5-9.5, and degumming temperature controls at 40 DEG C, and degummase liquid is kept stirring for state, and usually time is 1h. Measuring ramee uses compound enzyme according to data after PROCESS FOR TREATMENT of the present invention.
Table 2 present example 2 tests data for ramee
The continuous three crowdes of average/% of qualification rate Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 99 0.611 5.85 4.322 0.71 2081
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
Embodiment 3
By beta-glucosidase 4 grams, α-amylase 3 grams, trypsin 1 gram, alkaline pectase 12 grams, 1,4 beta-glucanase 30 grams, Neutral protease 14 grams, xylanase 15 grams, mannase 22 grams, bromelain 16 grams, erythritol 0.2 gram, 2-hydroxyl third Acid 0.3 gram, sodium citrate 0.35 gram, potassium sorbate 0.6 gram and attapulgite clay 29 grams, red mix homogeneously at normal temperatures, heating Stir to 36 DEG C, mixing time 1.5h, i.e. arrives compound enzyme.
Apply this example according to by Boehmeria binding, dress cage, enzyme refining, retted fibre, wash fiber crops, soda boiling, copy fiber crops, rinsing, oil supply, removing oil Water, trembling fiber crops, drying, obtain degummed ramie, described enzyme refining step, its main control parameters is: compound enzyme addition 1-3%, bath Liquor ratio is 1:15, pH8.5-9.5, and adding Disodium oxalate. is 1% and bentonite 2%, and degumming temperature controls at 40 DEG C, and degummase liquid is protected Holding stirring, usually time is 1h.Measuring ramee uses compound enzyme according to data after PROCESS FOR TREATMENT of the present invention.
Table 3 present example 3 tests data for ramee
The continuous three crowdes of average/% of qualification rate Residual gum content/% Fibre strength (gf/D) Runner rate/% Oil-containing/% Fibre count
Index of the present invention ≥ 98 0.52 6.78 3.98 0.72 2067
Certified products index ≥ 90 ＜ 2.8% ＞ 4.5 ＜ 10% 0.6~ 1.8 Nothing
After above-described embodiment, the ramee test data of gained are seen can be seen that, apply compound enzyme of the present invention to process Boehmeria Refining glue, the ramee property indices of gained is the most excellent.
Although the detailed description of the invention to the present invention gives detailed description and illustrates above, but it should be noted that We can carry out various equivalence according to the conception of the present invention to above-mentioned embodiment and change and amendment, and function produced by it is made With still without departing from description contained spiritual time, all should be within protection scope of the present invention.

Claims (5)

1. China grass degumming compound enzyme, it is characterised in that be composed of the following raw materials by weight:
Beta-glucosidase 2-5 part, α-amylase 2-5 part, trypsin 1-3 part, alkaline pectase 12-18 part, beta glucan Enzyme 30-36 part, neutral protease 10-15 part, xylanase 15-18 part, mannase 15-26 part, bromelain 15-22 part, Erythritol 0.1-0.4 part, 2 hydroxy propanoic acid 0.1-0.4 part, sodium citrate 0.1-0.5 part, potassium sorbate 0.2-0.8 part and recessed Convex rod clay 28-40 part.
China grass degumming compound enzyme the most according to claim 1, it is characterised in that be composed of the following raw materials by weight: β- Glucosidase 3 parts, α-amylase 4 parts, trypsin 2 parts, alkaline pectase 16 parts, 1,4 beta-glucanase 32 parts, neutral protein Enzyme 12 parts, xylanase 16 parts, mannase 19 parts, bromelain 18 parts, erythritol 0.3 part, 2 hydroxy propanoic acid 0.2 part, Sodium citrate 0.3 part, potassium sorbate 0.5 part and attapulgite clay 31 parts.
China grass degumming compound enzyme the most according to claim 1, it is characterised in that be composed of the following raw materials by weight: β- Glucosidase 2 parts, α-amylase 2 parts, trypsin 3 parts, alkaline pectase 15 parts, 1,4 beta-glucanase 33 parts, neutral protein Enzyme 11 parts, xylanase 17 parts, mannase 15 parts, bromelain 22 parts, erythritol 0.4 part, 2 hydroxy propanoic acid 0.1 part, Sodium citrate 0.14 part, potassium sorbate 0.3 part and attapulgite clay 38 parts.
China grass degumming compound enzyme the most according to claim 1, it is characterised in that be composed of the following raw materials by weight: β- Glucosidase 4 parts, α-amylase 3 parts, trypsin 1 part, alkaline pectase 12 parts, 1,4 beta-glucanase 30 parts, neutral protein Enzyme 14 parts, xylanase 15 parts, mannase 22 parts, bromelain 16 parts, erythritol 0.2 part, 2 hydroxy propanoic acid 0.3 part, Sodium citrate 0.35 part, potassium sorbate 0.6 part and attapulgite clay 29 parts.
5. according to the preparation method of the arbitrary described China grass degumming compound enzyme of claim 1-4, it is characterised in that by each raw material Mix homogeneously at normal temperatures, is heated to 35-40 DEG C and stirs, and mixing time 1-1.5h i.e. arrives compound enzyme.
CN201610702956.5A 2016-08-22 2016-08-22 Ramie degumming compound enzyme and preparation method thereof Pending CN106047842A (en)

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Application publication date: 20161026