CN101078003A - Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof - Google Patents
Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof Download PDFInfo
- Publication number
- CN101078003A CN101078003A CN 200610026795 CN200610026795A CN101078003A CN 101078003 A CN101078003 A CN 101078003A CN 200610026795 CN200610026795 CN 200610026795 CN 200610026795 A CN200610026795 A CN 200610026795A CN 101078003 A CN101078003 A CN 101078003A
- Authority
- CN
- China
- Prior art keywords
- preparation
- water
- bacteria
- water body
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to a kind of microbic reagent curing breed aquatics water by multi-bacteria. It can degrade and transform organic contamination of water, prohibite the growth of harmful algae and pathogeny bacteria, and promote propagation of beneficial creature, forming good entironment for growth of aquatic animal. Environment select breed and abduction select breed are adopted in this invention, spontaneous aberration and directional breed are combined, fermentated technology of original compound breed and domestication are used to make many bacteria of improved water mixed and matched each other. What' s more, growth factor and special carrier are added for promoting the growth of microbe, guaranteeing the efficiency of improvement. This product can take the place of antibiotic in feed, producing organic farm products which are green, safe and antibiotic-free.
Description
Technical field
The present invention relates to a kind of many bacterial classifications preparation for comprehensive treatment of cultivation water body that utilizes, be by in water body, directly adding, each bacterial classification effectively utilizes the organism of eutrophication in the water body, can decompose and transform the various organic contamination goods and materials in the water body rapidly, suppress the growth of harmful algae and pathogenic bacteria, promote beneficial organism breeding in the animal intestinal, form the optimum ecotope that is suitable for aquatic animal to grow.Belong to the agro-ecology product technique field.
Background technology
Flourish along with China aquaculture intensification, cultivation water environment pollutes serious day by day, because aquatic animal disease that environmental degradation causes or the communicable disease that causes thus increase severely in a large number, has become the major obstacle that the restriction culture fishery develops in a healthy way.Life-time service microbiotic and the control of other chemicalses are cultured disease and have been caused a series of environment and social concern, and its outstanding behaviours is microbiotic and the residual increase of harmful chemical in the product, and the fishery products price remains inactive over long periods of time.
Found that beneficial bacteria can improve the aquaculture water environment, promoted growth of animal in 1963.Parker1974 uses " Probiohc (s) " speech to describe the beneficial microorganism that uses to animal first, and it is defined as: help intestinal microflora equilibrated microorganism and material.In Hamburg, Germany symposial in 1994, scholars with Probiotics narrow sense be defined as " improve the balance of microorganism and enzyme, or stimulate specificity and nonspecific immunity mechanism viable bacteria and (or) dead bacterium (comprising component and product) ".Usefulness bacterium, light and bacteriums such as Austin, yeast and little algae are improved the microbial balance of aquatic animal internal and external environment, stimulate specificity and nonspecific immunity mechanism, promote growth, also they are called " Probiotics ".
Up to now, the most tangible bacterial classification of effect at middle improvement water body mainly contains photosynthetic bacterium, genus bacillus, milk-acid bacteria, actinomycetes etc., because they adhere to different genera separately, photosynthetic bacterium is photoautotrophy, and other kind is a chemoheterotrophy, genus bacillus is a spore production bacteria, and other kind is non-sporeformer, and growth conditions and nutritional needs difference are huge, and the microbial preparation of current improvement water body is of a great variety, but how based on single culture, and the effect of single culture only is embodied in a certain respect, is difficult to its effect is combined effectively in all conventional fermentations, and effect of integrated pest management is relatively poor, though the part composite fungus agent contains multiple bacterial classification, but content is lower, is about 1,000,000,000/gram, is difficult to form in water body dominant microflora, owing to the suitable inactivation of liquid preparation, improved effect is difficult to guarantee simultaneously.And current on the market photosynthetic bacteria is because the viable bacteria form of this bacterium is fine, proportion is little, if adopt the method for the aquaculture water of directly splashing, its viable bacteria is difficult for being deposited to pond bottom, can't play the good effect of improving the base ring border.
At present, the patent of having applied for, application number is CN02111869.8, the method that is entitled as the microcapsule life tonifying clean water composite bacteria repairing aquaculture environment, it is characterized in that: will be from multiple beneficial bacterial strains such as the isolated photosynthetic bacterium of nature, sporeformer, milk-acid bacterias, cultivate by combined fermentation process, handle, be re-dubbed a kind of microcapsule life tonifying clean water composite bacteria at last through freeze-drying or microcapsule.Because bacterial strain adheres to different kind (chemoheterotrophy and photoautotrophy, product gemma and non-product gemma) separately in the technology, the growth conditions difference is huge, and do not do the special cultivation of inducing and tame, be difficult in the spontaneous fermentation its combination, adopt microcapsule to granulate and lyophilize simultaneously, its thalline inactivation rate height, production cost are big, and improved effect is difficult to guarantee.
Summary of the invention
The purpose of this invention is to provide a kind of making between each bacterial classification mutually and coordinate, organic complementary, guarantee each bacterial strain in the product in water body, bring into play the best physiologically active utilize many bacterial classifications preparation for comprehensive treatment of cultivation water body and preparation method.
For realizing above purpose, technical scheme of the present invention provides a kind of many bacterial classifications preparation for comprehensive treatment of cultivation water body that utilizes, it is characterized in that, formulated by following weight percentages: Rhodopseudomonas palustris 25-32%, milk-acid bacteria 18-22%, saccharomyces cerevisiae 14-20%, subtilis 15-24%, actinomycetes 3-27%.
Preparation method of the present invention, it is characterized in that, adopt the probiotics of the multiple improvement water body of enrichment, utilization is induced seed selection to combine with post-directed training and is optimized seed selection, adopt compound cultivation to cultivate the fermentation technique that combines with domestication, interpolation is suitable for the symbiotic somatomedin of multiple thalline during the fermentation, adds the carrier that is suitable for the thalline long term in aftertreatment, the steps include:
The first step: the seed selection of producing bacterial strain
A. strain improvement: in the liquid of the wild carp enteron aisle of health, isolate plant lactobacillus, in the active sludge of sewage work, isolate subtilis, in the beer fermentation waste material, isolate saccharomyces cerevisiae, in the good shrimp pool of aquaculture water, isolate Rhodopseudomonas palustris, near sewage work, isolate actinomycetes in the soil, in the laboratory, make bacterial classification and preserve;
B. induce breeding: above five kinds of bacterial classifications are induced, carry out flat board dilution counting, select bodily form rule, fast growth, evident characteristic bacterial strain and preserved under ultraviolet irradiation;
C. bacterial classification shake flask test: rhodopseudomonas is inoculated sub-photosynthetic bacteria culture medium, plant lactobacillus is inoculated in the milk-acid bacteria substratum, saccharomyces cerevisiae is inoculated in MacConkey agar, subtilis is inoculated in the nutrient agar medium, under 35-40 ℃ of temperature, cultivated 22-28 hour, the separation of ruling, it is fast to select growth, thalline evident characteristic photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus, actinomycetes, in the laboratory, preserve, be inoculated in the above liquid nutrient medium, under 35-40 ℃ of temperature, cultivate 24-36 hour shake-flask culture, and live bacterial count arrives till 10 hundred million/gram at least;
D. the production production of hybrid seeds: bacterial classification transferred nutrient agar medium is housed does in the eggplant bottle of substratum, under 35-40 ℃ of temperature, cultivated 45-50 hour, treat that eggplant bottle surface lawn is covered with, and can take out when band is yellow in white.Putting into 2-6 ℃ refrigerator then preserves;
Second step: the independent and compound cultivation of useful bacterial strain
A. take by weighing by weight ratio by the good various bacterial classifications of the step 1 production production of hybrid seeds;
B. the compound cultivation of milk-acid bacteria, yeast, photosynthetic bacterium: with milk-acid bacteria, yeast, photosynthetic bacterium is the Rhodopseudomonas palustris combined inoculation in 120-130 ℃, the substratum of 30-45min high-temperature sterilization, in fermentor tank, stir 30min, its rotating speed is 200-240r/min, the plastic tank of putting into after stirring after 200L sterilizes left standstill 10-15 days, leave standstill the gas of measuring Ph value and release output in the process every day, prevent the animal feeds bucket explosion, fermentation ends when treating Ph arrival 3.0-4.0;
C. subtilis, actinomycetic compound cultivation: subtilis, actinomycetes combined inoculation are closed in the actinomycetic complex medium in 120-130 ℃, the withered grass gemma of 30-45min high-temperature sterilization, mixing speed is 200r/min-240r/min, culture temperature is 35-40 ℃, air flow 1: 1, fermentation time is 22-26 hour, and microscopically is observed when 90-95% gives birth to spore and finished;
The 3rd step: the domestication of flora and enlarged culturing
A. the bacterium liquid after will cultivating is inoculated in 120-130 ℃, the domestication and enlarged culturing base of 30-45min high-temperature sterilization by the 4-6% adding proportion, in fermentor tank, stir 30min, its rotating speed is 200-240r/min, the plastic tank of putting into after stirring after 200L sterilizes left standstill 10-15 days, leave standstill the gas of measuring Ph value and release output in the process every day, prevent the animal feeds bucket explosion, fermentation ends when treating Ph value arrival 3.0-4.0;
B. take a sample three times in the fermenting process, measure moisture and Ph value.Take a sample after the fermentation ends, measure moisture, Ph value and viable bacteria content;
The 4th step: add carrier
A. the enlarged culturing liquid that ferments adds 120-130 ℃, the carrier of 30-45min high-temperature sterilization in the 0.5-1% ratio, pours into to stir;
B. add the significant parameter of good leavened prod: total viable count 1,500,000,000-2,500,000,000/gram, pH value 3.0-4.0, physical behavior: Vandyke brown liquid contains a small amount of white depositions.
Described milk-acid bacteria, yeast, photosynthetic bacteria substratum are at least two kinds of mixtures in dregs of rice powder, urea, ammonium sulfate, wheat bran, yeast powder, Semen Maydis powder, molasses, potassium primary phosphate, Sodium phosphate dibasic, trimagnesium phosphate, sal epsom, the sterilized water, and its weight percent proportioning is: molasses 16-22%, sal epsom 0.5-1.5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, Sodium phosphate dibasic 0-1%, urea 1-3%, sterilized water 71.5-81.5%.
Described domestication and enlarged culturing base are at least two kinds of mixtures in dregs of rice powder, urea, ammonium sulfate, wheat bran, yeast powder, Semen Maydis powder, molasses, potassium primary phosphate, Sodium phosphate dibasic, trimagnesium phosphate, sal epsom, the sterilized water, and its weight percent proportioning is: dregs of beans 3-6%, ammonium sulfate 1-2%, yeast powder 1-3%, Semen Maydis powder 6-10%, molasses 12-20%, Sodium phosphate dibasic 0-1%, trimagnesium phosphate 0-1%, production factor solution 0.8-1%, sterilized water 57-77%.
Described somatomedin concentration is to add 6 water iron(ic) chloride 4-6g, cupric sulfate pentahydrate 0.004-0.06g, boric acid 0.8-1g, 7 water zinc sulphate 0.8-1g, Xiao Suangu 0.4-0.5g, 4 water Manganous chloride tetrahydrate 0.05g in 1000ml water.
Described production the 4th step interpolation carrier is made up of with lime carbonate oblique native waste stone dust and feed, and its weight percent proportioning is zeolite powder 70-90%, lime carbonate 10-30%.
Described complex medium be molasses 16-22%, sal epsom o.5-1.5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, Sodium phosphate dibasic 0-1%, urea 1-3%, sterilized water 71.5-81.5%.
The present invention adopts advanced environment seed selection and the technology of inducing seed selection to combine, spontaneous mutation and post-directed training are combined, adopt the compound cultivation of original creation and the fermentation technique that domestication combines simultaneously, make between each bacterial classification and coordinate mutually, organic complementary, simultaneously, the present invention adds in cultivation and is suitable for the symbiotic special somatomedin of thalline and is suitable for the special carrier that thalline plays a role, utilize beneficial microorganism to absorb ammonia nitrogen at water body, nitrous acid nitrogen and hydrogen sulfide etc., effectively decompose larger molecular organics, suppress a large amount of breedings of pathogenic bacterium simultaneously, to guarantee that each bacterial strain is brought into play best physiologically active in the product in water body.
Photosynthetic bacterium is that Rhodopseudomonas palustris is a gram negative bacterium, can grow under the condition that the light anaerobic is arranged, breed, and also can grow under the condition of unglazed aerobic.There is the light time thalline can utilize luminous energy, with H
2S and organism grow as carbon source with CO2 or organism as hydrogen donor.Photosynthetic bacterium can absorb the objectionable impurities in the water body, grow up to own virtuous cell, and formation advantage group, suppress the pathogeny microorganism growth, purify water, the photosynthetic bacterium of this patent is owing to added the settled carrier of suitable thalline, make the viable bacteria form of this bacterium sturdy, than great, the aquaculture water of directly splashing, its viable bacteria is deposited to pond bottom easily, can play the good effect of improving the base ring border.
Lactobacillus belongs to the anaerobies population, and lactic acid has germicidal action, can suppress the harmful microorganism activity, pathogenic bacterium and organism corruption, and organism such as xylogen, VITAMIN are decomposed, help animals and plants and absorb.
Yeast flora belongs to the aerobe group, can provide propagation matrix for milk-acid bacteria, actinomycetes etc., for animals and plants provide single cell protein.
Gram positive actinomycetes belongs to the aerobe group, can obtain matrix from photosynthetic bacterium, produces various microbiotic, VITAMIN and enzyme, directly suppresses pathogenic bacteria propagation, promotes beneficial microorganism propagation.Actinomycetes and photosynthetic bacterium mix uses better.
Genus bacillus is an aerobic bacteria, when the aquaculture water dissolved oxygen is high, its reproduction speed is accelerated, the efficient of decomposing larger molecular organics improves, therefore in this bacterium of splashing, start oxygenating machine, can make it form the population advantage rapidly in the water body breeding, because genus bacillus is a chemoheterotrophic bacteria, therefore when the deterioration of aquaculture water bottom environment, when algae is not good mutually, can use genus bacillus as early as possible, genus bacillus of the present invention is dense, can utilize larger molecular organics matter rapidly, the organic substance mineralising can be generated inorganic salt simultaneously and provide nutrition for unicellular algae, the photosynthesis of unicellular algae is again organic oxidation, the breathing of microorganism, the breathing of aquatic animal provides oxygen, moves in circles, constitute a benign ecological circulation, make the bacterium in the pond reach balance mutually with algae mutually, keep stable water colour, build good bottom environment.
This patent product carries out complex ferment according to the growth characteristics, and total viable count reaches more than 2,000,000,000/gram, has added the carrier of each bacterial classification effect simultaneously, has guaranteed improved effect.This product has been listed national Spark Plan project product in, also is 863 Program project product simultaneously, at present in Shanghai, the big provinces of aquaculture such as Guangdong, Zhejiang, Jiangsu apply, and have obtained good culture benefit and social benefit.
Advantage of the present invention is:
1, adopts environment seed selection and induce breeding technique, spontaneous mutation and post-directed training are combined, guaranteed the high reactivity and the high yield of purpose bacterial strain;
2, with compound cultivation and the fermentation technique that domestication combines, both guaranteed the high-speed rapid growth of probiotics, guaranteed that again its meta-bolites keeps fully, guaranteed the effect of product to greatest extent to aquaculture water;
3, rhodopseudomonas effectively utilizes organic nutriment of enrichment in the water body in the product, suppress the growth of harmful algae, genus bacillus and actinomycetes produce special bacitracin, can from photosynthetic bacterium, obtain matrix, produce various microbiotic, VITAMIN and enzyme, directly suppress pathogenic bacteria propagation, cereuisiae fermentum produces abundant single cell protein, guarantee the high-speed rapid growth of cultivated animals, lactic acid bacteria class is by the cultivated animals intestinal absorption, keep the balance of profitable strain in the enteron aisle, produce the pH value that lactic acid, acetate, propionic acid etc. reduce organismic internal environment; These bacterial classifications interact, and influence each other, and the best that keeps water body to be in " fertile, alive, tender, refreshing " is cultured state;
4, in water body, grow and the effect characteristics according to each bacterial classification, add synthetic required somatomedin and the carrier of rich trace elements and thalline, solved shortcomings such as single culture viable bacteria content in breed is low, the effect time length is short, guaranteed continuing of product effect with efficient;
5, the probiotics of the occurring in nature seed selection adopted of this patent meets feedstuff industry 318 bacterial classifications and adds standard, can significantly reduce and alternative feed in antibiotic use, produce green, safety, the residual organic farm products of antibiotic-free;
6, adopt food crop and common feedstuffs carrier as the main medium raw material, reduced production cost, use fermentation and production unit commonly used simultaneously, be easy in aquaculture, promote.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
The first step: the seed selection of producing bacterial classification
A, strain improvement: in the wild carp intestinal secretion thing of health, utilize the milk-acid bacteria substratum to isolate plant lactobacillus, in the active sludge of sewage work, utilize nutrient agar to isolate subtilis, in the beer fermentation waste material, utilize yeast to select substratum to isolate saccharomyces cerevisiae, in the good shrimp pool of aquaculture water, utilize photosynthetic bacteria culture medium to isolate rhodopseudomonas, nutrient agar is isolated actinomycetes in the soil near sewage work, makes bacterial classification and preserve in the laboratory; The present invention adopts following bacterial classification:
Rhodopseudomonas palustris (ATCC17001) Rhodopseudomonas palustris, Lactobacillusplantarum (ATCC8014) plant lactobacillus, Saccharomyces cerevisiae (IFO0203) saccharomyces cerevisiae, Bacillus subtilis (1.398) subtilis, Nocardia Nocardia bacteria;
B. induce breeding: with above five kinds of bacterial classifications power under the ultraviolet ray be 15W, distance for the irradiation of the UV-light of 30cm under 30min, induce, bacteria suspension after inducing carries out flat board dilution counting, selects bodily form rule, fast growth, the preservation of evident characteristic bacterial strain.
C. bacterial classification shake flask test: Rhodopseudomonas palustris is inoculated in photosynthetic bacteria culture medium, plant breast bar is inoculated in Lactic-bacteria Medium I (milk-acid bacteria substratum), saccharomyces cerevisiae is inoculated in Maxwell (Meclary) agar, subtilis is inoculated in the nutrient agar medium (LB substratum), under 35 ℃ of temperature, cultivated 22-28 hour, carrying out setting-out separates, it is fast to select growth, thalline evident characteristic photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus, actinomycetes, in the laboratory, preserve, be inoculated in the above liquid nutrient medium, under 35 ℃ of temperature, carry out 24-36 hour shake-flask culture, carry out live bacterial count and arrive till at least 10 hundred million/gram;
Each screening culture medium component:
Milk-acid bacteria substratum: yeast extract paste 7.5g, peptone 7.5g, glucose) 10g, KH2PO42g, the 100ml of Tomato juice, Tween (tween) 800.5ml, Disti distilled water) 900ml pH7.0;
Yeast is selected substratum: glucose 1g, KCl1.8g, yeast extract 2.5g, sodium-acetate 8.2g, agar 15-20g, distilled water 1000ml;
Nutrient agar: extractum carnis 3g, peptone 10g, NaCl5g, agar 15-20g, water 1000ml, pH7.0-7.2;
MacConkey agar is glucose 1g, KCI 1.8g, yeast extract 2.5g, sodium-acetate 8.2g, agar 15-20g, distilled water 1000ml;
Photosynthetic bacteria substratum: potassium primary phosphate 0.5g, ammonium sulfate 1.0g, 7 water magnesium sulfate 0.2g, sodium-chlor 0.2g, 2 water calcium chloride 0.05g, yeast extract paste 0.1g, peptone 0.5g, sodium-acetate 0.5g, Sodium.alpha.-ketopropionate 0.5g, growth factor solution 1mL, trace element solution 1mL, distilled water 1000ml
These substratum are all on the books in the laboratory operation book, for conventionally known to one of skill in the art;
D, the production production of hybrid seeds: bacterial classification transferred respectively nutrient agar medium is housed does in the eggplant bottle of substratum, under 35-40 ℃ of temperature, cultivated 45-50 hour, treat that eggplant bottle surface lawn is covered with, and can take out when band is yellow in white, put into 2-6 ℃ refrigerator then and preserve;
Second step: the independent and compound cultivation of useful bacterial strain
A. take by weighing the production bacterial classification by weight ratio: Rhodopseudomonas palustris 26%, milk-acid bacteria 18%, saccharomyces cerevisiae 15%, subtilis 15%, actinomycetes 26%;
B. milk-acid bacteria, yeast, photosynthetic bacterium are the compound cultivation of Rhodopseudomonas palustris: lawn in the eggplant bottle is inoculated in 121 ℃, the substratum of 30min high-temperature sterilization, in fermentor tank, stir 30min, its rotating speed is 200r/min, the plastic tank of putting into after stirring after 200L sterilizes left standstill 14 days, leave standstill the gas of measuring Ph value and release output in the process every day, prevent the animal feeds bucket explosion, treat that Ph arrives 3.5 o'clock fermentation ends.
C. subtilis, actinomycetic compound cultivation: eggplant bottle lawn is inoculated in 121 ℃, the complex medium of 30min high-temperature sterilization, mixing speed is 200r/min r/min, culture temperature is 35 ℃, air flow 1: 1, fermentation time is 28 hours, and microscopically is observed when 90-95% gives birth to spore and finished;
Milk-acid bacteria, yeast, photosynthetic bacteria complex medium: molasses 16%, sal epsom 0.5%, ammonium sulfate 1%, potassium primary phosphate 0.5%, Sodium phosphate dibasic 0.5%, urea 1%, sterilized water 80.5%.Subtilis, actinomycetic complex medium: bean cake powder 2%, Semen Maydis powder 3%, potassium primary phosphate 0.4%, SODIUM PHOSPHATE, MONOBASIC 1.6%, sal epsom 0.6%, urea 1.5%, molasses 0.1%, sterilized water 90.8%.
The 3rd step: the domestication of flora and enlarged culturing
Bacterium liquid after the domestication is owing to grow in different yeastings, so though concentration height, growth are fast, but the speed of growth descends in same substratum, need carry out the domestication of same substratum, simultaneously, add growth factor solution during the fermentation, promote the bacterial classification high-speed rapid growth, so the present invention carries out the domestication and the enlarged culturing of flora after strain culturing, its specific practice is:
A. the bacterium liquid after will cultivating is inoculated in 121 ℃, the domestication and enlarged culturing base of 45min high-temperature sterilization by 4% adding proportion, stirs 30min in fermentor tank, and its rotating speed is 220r/min, adds the somatomedin of 0.1% ratio simultaneously.The plastic tank of putting into after stirring after 200L sterilizes left standstill 12 days, left standstill the gas of measuring Ph value and release output in the process every day, prevented the animal feeds bucket explosion, treated that Ph arrives 3.5 o'clock fermentation ends;
B. take a sample three times in the fermenting process, measure moisture and Ph value.Take a sample after the fermentation ends, measure moisture, Ph value and viable bacteria content;
Domestication and enlarged culturing base are: dregs of beans 3%, ammonium sulfate 1%, yeast powder 1%, Semen Maydis powder 6%, molasses 12%, Sodium phosphate dibasic 0.5%, trimagnesium phosphate 0.5%, sterilized water 77%;
Growth factor solution (adding in the 1000ml water): Iron trichloride hexahydrate 5g, cupric sulfate pentahydrate 0.05g, boric acid 1g, 7 water zinc sulphate 1g, Xiao Suangu 0.5g, 4 water Manganous chloride tetrahydrate 0.05g, constant volume adds in the enlarged culturing base in 0.1% ratio in the 1kg sterilized water;
The 4th step: add carrier
A. the expansion liquid that ferments is the carrier that 200 purposes are made up of zeolite powder 80%, lime carbonate 20% in 121 ℃ of 0.5% ratio interpolations, 45min high-temperature sterilization granularity, pours into to stir.
B. add the significant parameter of good leavened prod; Total viable count 2,000,000,000/gram, pH value 3.5-4.0, physical form: Vandyke brown liquid, special fermenting aroma is arranged, contain a small amount of white depositions.
Embodiment 2
The first step: the seed selection of producing bacterial classification
With embodiment 1
Second step: the independent and compound cultivation of useful bacterial strain
A. take by weighing the production bacterial classification by weight ratio: Rhodopseudomonas palustris 30%, milk-acid bacteria 20%, saccharomyces cerevisiae 18%, subtilis 20%, actinomycetes 12%;
The compound cultivation of b, milk-acid bacteria, yeast, photosynthetic bacterium: lawn in the eggplant bottle is inoculated in 120 ℃, the substratum of 40min high-temperature sterilization, in fermentor tank, stir 30min, its rotating speed is 200r/min, the plastic tank of putting into after stirring after 200L sterilizes left standstill 14 days, leave standstill the gas of measuring Ph value and release output in the process every day, prevent the animal feeds bucket explosion, treat that Ph arrives 3.5 o'clock fermentation ends.
C, subtilis, actinomycetic compound cultivation: eggplant bottle lawn is inoculated in 120 ℃, the substratum of 40min high-temperature sterilization, mixing speed is 200r/min r/min, culture temperature is 35 ℃, air flow 1: 0.8, fermentation time is 28 hours, and microscopically is observed when 90-95% gives birth to spore and finished; Milk-acid bacteria, yeast, photosynthetic bacteria complex medium: molasses 16-22%, sal epsom o.5-1.5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, Sodium phosphate dibasic 0-1%, urea 1-3%, sterilized water 71.5-81.5%.Subtilis, actinomycetic complex medium are with example 1;
The domestication and the enlarged culturing of the 3rd step, flora
A, with embodiment 1;
The ratio of adding strain liquid is 6%, domestication and enlarged culturing base: dregs of beans 6%, ammonium sulfate 2%, yeast powder 3%, Semen Maydis powder 9%, molasses 18%, Sodium phosphate dibasic 1%, trimagnesium phosphate 1%, sterilized water 60%; Sampling is three times in b, the fermenting process, measures moisture and Ph value.Take a sample after the fermentation ends, measure moisture, Ph value and viable bacteria content.
The 4th step, interpolation carrier
It is 1: 1 carrier by oblique native waste stone dust and feed calcium carbonate proportion that a, the enlarged culturing liquid that ferments add 121 ℃, 45min high-temperature sterilization in 1% ratio, and granularity is 200 orders, pours in the stirrer to stir.
B, with embodiment 1;
The present invention combines the most tangible several microbial strainss of effect in the water body improvement at present, breeding and domestication complex ferment technology are induced in employing, improve its activity and proliferation rate in water body greatly, added the special carrier that is suitable for the thalline effect simultaneously, guaranteed the lasting of improved effect.
Culture the pool by every 10-15 mu during use, 1 meter depth of water, this product 1kg splashes, aquaculture water will be clear by turbid change after using, culture in the popularization experiment at aquatic products station at the Shanghai Nanhui District, the ammonia-nitrogen content average specific control group on the test pool has reduced by 70%, and nitrite has reduced by 54%, and dissolved oxygen has improved 9.4%.Harmful algae quantity obviously reduces by 200/ml, and water body becomes grass green by muddy green.
Claims (7)
1. one kind is utilized many bacterial classifications preparation for comprehensive treatment of cultivation water body, it is characterized in that, formulated by following weight percentages: Rhodopseudomonas palustris 25-32%, milk-acid bacteria 18-22%, saccharomyces cerevisiae 15-20%, subtilis 15-23%, actinomycetes 4-26%.
2. a kind of preparation method who utilizes many bacterial classifications preparation for comprehensive treatment of cultivation water body according to claim 1, it is characterized in that, adopt the probiotics of the multiple improvement water body of enrichment, utilization is induced seed selection to combine with post-directed training and is optimized seed selection, adopt compound cultivation to cultivate the fermentation technique that combines with domestication, interpolation is suitable for the symbiotic somatomedin of multiple thalline during the fermentation, adds the carrier that is suitable for the thalline long term in aftertreatment, the steps include:
The first step: the seed selection of producing bacterial strain
A. strain improvement: in the wild carp intestinal secretion thing of health, utilize the milk-acid bacteria substratum to isolate plant lactobacillus, in the active sludge of sewage work, utilize nutrient agar to isolate subtilis, in the beer fermentation waste material, utilize yeast to select substratum to isolate saccharomyces cerevisiae, in the good shrimp pool of aquaculture water, utilize photosynthetic bacteria culture medium to isolate rhodopseudomonas, nutrient agar is isolated actinomycetes in the soil near sewage work, makes bacterial classification and preserve in the laboratory;
B. induce breeding: above five kinds of bacterial classifications are induced, carry out flat board dilution counting, select bodily form rule, fast growth, evident characteristic bacterial strain and preserved under ultraviolet irradiation;
C. bacterial classification shake flask test: rhodopseudomonas is inoculated in photosynthetic bacteria culture medium, plant lactobacillus is inoculated in the milk-acid bacteria substratum, saccharomyces cerevisiae is inoculated in MacConkey agar, subtilis is inoculated in the nutrient agar medium, under 35-40 ℃ of temperature, cultivated 22-28 hour, the separation of ruling, it is fast to select growth, thalline evident characteristic photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus, actinomycetes, in the laboratory, preserve, be inoculated in the above liquid nutrient medium, under 35-40 ℃ of temperature, cultivate 24-36 hour shake-flask culture, and live bacterial count arrives till 10 hundred million/gram at least;
D. the production production of hybrid seeds: bacterial classification transferred nutrient agar medium is housed does in the eggplant bottle of substratum, under 35-40 ℃ of temperature, cultivated 45-50 hour, treat that eggplant bottle surface lawn is covered with, and can take out when band is yellow in white.Putting into 2-6 ℃ refrigerator then preserves;
Second step: the independent and compound cultivation of useful bacterial strain
A. take by weighing by weight ratio by the good various bacterial classifications of the step 1 production production of hybrid seeds;
B. the compound cultivation of milk-acid bacteria, yeast, photosynthetic bacterium: with milk-acid bacteria, yeast, photosynthetic bacterium is the Rhodopseudomonas palustris combined inoculation in 120-130 ℃, the substratum of 30-45min high-temperature sterilization, in fermentor tank, stir 30min, its rotating speed is 200-240r/min, the plastic tank of putting into after stirring after 200L sterilizes left standstill 10-15 days, leave standstill the gas of measuring Ph value and release output in the process every day, prevent the animal feeds bucket explosion, fermentation ends when treating Ph arrival 3.0-4.0;
C. subtilis, actinomycetic compound cultivation: subtilis, actinomycetes combined inoculation are closed in the actinomycetic complex medium in 120-130 ℃, the withered grass gemma of 30-45min high-temperature sterilization, mixing speed is 200r/min-240r/min, culture temperature is 35-40 ℃, air flow 1: 1, fermentation time is 22-26 hour, and microscopically is observed when 90-95% gives birth to spore and finished;
The 3rd step: the domestication of flora and enlarged culturing
A. the bacterium liquid after will cultivating is inoculated in 120-130 ℃, the domestication and enlarged culturing base of 30-45min high-temperature sterilization by the 4-6% adding proportion, in fermentor tank, stir 30min, its rotating speed is 200-240r/min, the plastic tank of putting into after stirring after 200L sterilizes left standstill 10-15 days, leave standstill the gas of measuring Ph value and release output in the process every day, prevent the animal feeds bucket explosion, fermentation ends when treating Ph value arrival 3.0-4.0;
B. take a sample three times in the fermenting process, measure moisture and Ph value.Take a sample after the fermentation ends, measure moisture, Ph value and viable bacteria content;
The 4th step: add carrier
A. the enlarged culturing liquid that ferments adds 120-130 ℃, the carrier of 30-45min high-temperature sterilization in the 0.5-1% ratio, pours into to stir;
B. add the significant parameter of good leavened prod: total viable count 1,500,000,000-2,500,000,000/gram, pH value 3.0-4.0, physical behavior: Vandyke brown liquid contains a small amount of white depositions.
3. a kind of preparation method who utilizes many bacterial classifications preparation for comprehensive treatment of cultivation water body according to claim 2, it is characterized in that, described milk-acid bacteria, yeast, the photosynthetic bacteria substratum is sterilized water and dregs of rice powder, urea, ammonium sulfate, wheat bran, yeast powder, Semen Maydis powder, molasses, potassium primary phosphate, Sodium phosphate dibasic, trimagnesium phosphate, at least two kinds of mixtures in the sal epsom, its weight percent proportioning is: molasses 16-22%, sal epsom 0.5-1.5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, Sodium phosphate dibasic 0-1%, urea 1-3%, sterilized water 71.5-81.5%.
4. a kind of preparation method who utilizes many bacterial classifications preparation for comprehensive treatment of cultivation water body according to claim 2, it is characterized in that, described domestication and enlarged culturing base are sterilized water and dregs of rice powder, urea, ammonium sulfate, wheat bran, yeast powder, Semen Maydis powder, molasses, potassium primary phosphate, Sodium phosphate dibasic, trimagnesium phosphate, at least two kinds of mixtures in the sal epsom, its weight percent proportioning is: dregs of beans 3-6%, ammonium sulfate 1-2%, yeast powder 1-3%, Semen Maydis powder 6-10%, molasses 12-20%, Sodium phosphate dibasic 0-1%, trimagnesium phosphate 0-1%, production factor solution 0.8-1%, sterilized water 57-77%.
5. a kind of many bacterial classifications preparation for comprehensive treatment of cultivation water body that utilizes according to claim 2, it is characterized in that described somatomedin concentration is to add 6 water iron(ic) chloride 4-6g, cupric sulfate pentahydrate 0.004-0.06g, boric acid 0.8-1g, 7 water zinc sulphate 0.8-1g, Xiao Suangu 0.4-0.5g, 4 water Manganous chloride tetrahydrate 0.05g in 1000ml water.
6. a kind of many bacterial classifications preparation for comprehensive treatment of cultivation water body that utilizes according to claim 2, it is characterized in that, described production the 4th step interpolation carrier is made up of with lime carbonate oblique native waste stone dust and feed, and its weight percent proportioning is zeolite powder 70-90%, lime carbonate 10-30%.
7. a kind of many bacterial classifications preparation for comprehensive treatment of cultivation water body that utilizes according to claim 2, it is characterized in that described complex medium is molasses 16-22%, sal epsom 0.5-1.5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, Sodium phosphate dibasic 0-1%, urea 1-3%, sterilized water 71.5-81.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610026795 CN101078003A (en) | 2006-05-23 | 2006-05-23 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610026795 CN101078003A (en) | 2006-05-23 | 2006-05-23 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101078003A true CN101078003A (en) | 2007-11-28 |
Family
ID=38905673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610026795 Pending CN101078003A (en) | 2006-05-23 | 2006-05-23 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101078003A (en) |
Cited By (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948757A (en) * | 2010-09-30 | 2011-01-19 | 大连三科生物工程有限公司 | Composite microbial additive for aquatic products, preparation method thereof and use thereof |
| WO2011063597A1 (en) * | 2009-11-25 | 2011-06-03 | Wang Liping | Complex microbial preparation for treating diabetes and preparative method and use thereof |
| CN102258796A (en) * | 2011-06-28 | 2011-11-30 | 黄小娃 | Microbial deodorant and preparation method thereof |
| CN101536736B (en) * | 2009-04-25 | 2012-06-13 | 陈家珍 | Special fishing fertilizer for aquaculture and preparation method thereof |
| CN102531198A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Water body cleansing agent |
| CN102703359A (en) * | 2012-06-12 | 2012-10-03 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| CN103193329A (en) * | 2013-04-15 | 2013-07-10 | 中国水产科学研究院淡水渔业研究中心 | Biological agent for controlling excessive propagation of blue-green algae of aquaculture water and preparation method of biological agent |
| CN103539267A (en) * | 2013-11-06 | 2014-01-29 | 广东宏益鳄鱼产业有限公司 | Method for improving water quality of alligator pond |
| CN103583880A (en) * | 2013-10-18 | 2014-02-19 | 上海永凡生物科技有限公司 | Microbial fermentation feed for litopenaeus vannamei |
| CN103966130A (en) * | 2014-05-04 | 2014-08-06 | 上海创博生物工程有限公司 | Stress-resistant microbial preparation for prawn, and preparation method of microbial preparation |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN104498382A (en) * | 2014-09-30 | 2015-04-08 | 国家***第三海洋研究所 | Microbial preparation for improving aquatic product culture environment and preparation method thereof |
| CN104488969A (en) * | 2014-11-03 | 2015-04-08 | 沧州旺发生物技术研究所有限公司 | Microecological preparation for inhibiting blue-green algae |
| CN104845910A (en) * | 2015-05-13 | 2015-08-19 | 河海大学 | Biochemical reagent and preparation method and application thereof |
| CN105174654A (en) * | 2015-10-16 | 2015-12-23 | 中国科学院水生生物研究所 | Deodorization method for landscape water body |
| CN105211104A (en) * | 2015-08-28 | 2016-01-06 | 上海创博生态工程有限公司 | A kind of preparation method of pet breeding environment modifying agent |
| CN105347511A (en) * | 2015-11-30 | 2016-02-24 | 威海地宝生物科技有限公司 | Compound microbial preparation for aquaculture and method |
| CN105439380A (en) * | 2015-12-12 | 2016-03-30 | 常州大学 | Method for removing organic matters in salt-containing waste water |
| CN105454731A (en) * | 2015-12-28 | 2016-04-06 | 上海创博生态工程有限公司 | Microorganism feed dedicated for Trachidermus fasciatus Heckel and preparation method thereof |
| CN106234842A (en) * | 2016-08-03 | 2016-12-21 | 大连海洋大学 | Industrialized culture feed for prawn and preparation method thereof |
| CN106915828A (en) * | 2017-04-05 | 2017-07-04 | 新疆金兰德泰环保科技有限公司 | A kind of water remediation microbial inoculum and preparation method thereof |
| CN108424872A (en) * | 2018-03-09 | 2018-08-21 | 江西润垚生物科技有限公司 | A kind of preparation method of aquaculture composite bacteria agent |
| CN108813223A (en) * | 2018-07-03 | 2018-11-16 | 珠海市西科水产养殖有限公司 | A kind of aquaculture specific complex microorganism formulation and preparation method thereof |
| CN109607819A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of control of microorganisms aquaculture method |
| CN110122404A (en) * | 2019-04-15 | 2019-08-16 | 威海格润环保科技股份有限公司 | A kind of ocean bottom holothurian cultivation oxygen supply purification device and method |
| CN110127827A (en) * | 2019-05-30 | 2019-08-16 | 田其珍 | A kind of preparation method of antibacterial high adsorption flocculant |
| EP3572500A1 (en) * | 2011-01-12 | 2019-11-27 | Concentric Ag Corporation | Microbial compositions and methods |
| CN110832067A (en) * | 2017-07-03 | 2020-02-21 | 橡树生物公司 | Novel microbial biomass based feed products |
| CN111040978A (en) * | 2020-01-14 | 2020-04-21 | 江阴市赈兴生物工程有限公司 | Probiotic fertilizer water product based on aquaculture environment biological domestication and preparation method thereof |
| CN111040970A (en) * | 2019-12-27 | 2020-04-21 | 河北九华勘查测绘有限责任公司 | Compound microbial agent, preparation method thereof and application thereof in black and odorous water body remediation |
| CN111316941A (en) * | 2020-03-03 | 2020-06-23 | 中国水产科学研究院珠江水产研究所 | Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings |
| CN112617027A (en) * | 2020-12-31 | 2021-04-09 | 上海泓宝绿色水产股份有限公司 | Bdelloecium bdellovii and preparation method and use method thereof |
| CN113461179A (en) * | 2021-08-06 | 2021-10-01 | 南京农业大学 | Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment |
| CN113750778A (en) * | 2021-10-14 | 2021-12-07 | 济宁市金益菌生物科技有限公司 | Preparation method of biological deodorant and biological deodorant |
-
2006
- 2006-05-23 CN CN 200610026795 patent/CN101078003A/en active Pending
Cited By (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536736B (en) * | 2009-04-25 | 2012-06-13 | 陈家珍 | Special fishing fertilizer for aquaculture and preparation method thereof |
| WO2011063597A1 (en) * | 2009-11-25 | 2011-06-03 | Wang Liping | Complex microbial preparation for treating diabetes and preparative method and use thereof |
| CN101948757A (en) * | 2010-09-30 | 2011-01-19 | 大连三科生物工程有限公司 | Composite microbial additive for aquatic products, preparation method thereof and use thereof |
| EP3572500A1 (en) * | 2011-01-12 | 2019-11-27 | Concentric Ag Corporation | Microbial compositions and methods |
| CN102258796A (en) * | 2011-06-28 | 2011-11-30 | 黄小娃 | Microbial deodorant and preparation method thereof |
| CN102531198A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Water body cleansing agent |
| CN102703359A (en) * | 2012-06-12 | 2012-10-03 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| CN102703359B (en) * | 2012-06-12 | 2014-11-26 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| CN103193329A (en) * | 2013-04-15 | 2013-07-10 | 中国水产科学研究院淡水渔业研究中心 | Biological agent for controlling excessive propagation of blue-green algae of aquaculture water and preparation method of biological agent |
| CN103193329B (en) * | 2013-04-15 | 2014-06-18 | 中国水产科学研究院淡水渔业研究中心 | Biological agent for controlling excessive propagation of blue-green algae of aquaculture water and preparation method of biological agent |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN104232497B (en) * | 2013-06-19 | 2019-01-15 | 上海泓宝绿色水产股份有限公司 | A kind of compound EM flora preparation and preparation method thereof for improving water quality of multi-cultur es |
| CN103583880A (en) * | 2013-10-18 | 2014-02-19 | 上海永凡生物科技有限公司 | Microbial fermentation feed for litopenaeus vannamei |
| CN103583880B (en) * | 2013-10-18 | 2015-04-08 | 上海永凡生物科技有限公司 | Microbial fermentation feed for litopenaeus vannamei |
| CN103539267B (en) * | 2013-11-06 | 2016-01-13 | 广东宏益鳄鱼产业有限公司 | The method of a kind crocodile pond water quality improvement |
| CN103539267A (en) * | 2013-11-06 | 2014-01-29 | 广东宏益鳄鱼产业有限公司 | Method for improving water quality of alligator pond |
| CN103966130A (en) * | 2014-05-04 | 2014-08-06 | 上海创博生物工程有限公司 | Stress-resistant microbial preparation for prawn, and preparation method of microbial preparation |
| CN104498382A (en) * | 2014-09-30 | 2015-04-08 | 国家***第三海洋研究所 | Microbial preparation for improving aquatic product culture environment and preparation method thereof |
| CN104498382B (en) * | 2014-09-30 | 2017-10-20 | 国家***第三海洋研究所 | A kind of microorganism formulation improved applied to culture environment of aquatic products and preparation method thereof |
| CN104488969B (en) * | 2014-11-03 | 2017-10-03 | 沧州旺发生物技术研究所有限公司 | A kind of probiotics for suppressing blue-green algae |
| CN104488969A (en) * | 2014-11-03 | 2015-04-08 | 沧州旺发生物技术研究所有限公司 | Microecological preparation for inhibiting blue-green algae |
| CN104845910A (en) * | 2015-05-13 | 2015-08-19 | 河海大学 | Biochemical reagent and preparation method and application thereof |
| CN105211104A (en) * | 2015-08-28 | 2016-01-06 | 上海创博生态工程有限公司 | A kind of preparation method of pet breeding environment modifying agent |
| CN105174654B (en) * | 2015-10-16 | 2018-08-21 | 中国科学院水生生物研究所 | A method of being used for landscape water body deodorization |
| CN105174654A (en) * | 2015-10-16 | 2015-12-23 | 中国科学院水生生物研究所 | Deodorization method for landscape water body |
| CN105347511A (en) * | 2015-11-30 | 2016-02-24 | 威海地宝生物科技有限公司 | Compound microbial preparation for aquaculture and method |
| CN105439380A (en) * | 2015-12-12 | 2016-03-30 | 常州大学 | Method for removing organic matters in salt-containing waste water |
| CN105454731A (en) * | 2015-12-28 | 2016-04-06 | 上海创博生态工程有限公司 | Microorganism feed dedicated for Trachidermus fasciatus Heckel and preparation method thereof |
| CN106234842A (en) * | 2016-08-03 | 2016-12-21 | 大连海洋大学 | Industrialized culture feed for prawn and preparation method thereof |
| CN106915828A (en) * | 2017-04-05 | 2017-07-04 | 新疆金兰德泰环保科技有限公司 | A kind of water remediation microbial inoculum and preparation method thereof |
| CN110832067A (en) * | 2017-07-03 | 2020-02-21 | 橡树生物公司 | Novel microbial biomass based feed products |
| CN108424872A (en) * | 2018-03-09 | 2018-08-21 | 江西润垚生物科技有限公司 | A kind of preparation method of aquaculture composite bacteria agent |
| CN108813223A (en) * | 2018-07-03 | 2018-11-16 | 珠海市西科水产养殖有限公司 | A kind of aquaculture specific complex microorganism formulation and preparation method thereof |
| CN109607819A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of control of microorganisms aquaculture method |
| CN110122404A (en) * | 2019-04-15 | 2019-08-16 | 威海格润环保科技股份有限公司 | A kind of ocean bottom holothurian cultivation oxygen supply purification device and method |
| CN110127827A (en) * | 2019-05-30 | 2019-08-16 | 田其珍 | A kind of preparation method of antibacterial high adsorption flocculant |
| CN111040970A (en) * | 2019-12-27 | 2020-04-21 | 河北九华勘查测绘有限责任公司 | Compound microbial agent, preparation method thereof and application thereof in black and odorous water body remediation |
| CN111040978A (en) * | 2020-01-14 | 2020-04-21 | 江阴市赈兴生物工程有限公司 | Probiotic fertilizer water product based on aquaculture environment biological domestication and preparation method thereof |
| CN111316941A (en) * | 2020-03-03 | 2020-06-23 | 中国水产科学研究院珠江水产研究所 | Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings |
| CN112617027A (en) * | 2020-12-31 | 2021-04-09 | 上海泓宝绿色水产股份有限公司 | Bdelloecium bdellovii and preparation method and use method thereof |
| CN113461179A (en) * | 2021-08-06 | 2021-10-01 | 南京农业大学 | Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment |
| CN113750778A (en) * | 2021-10-14 | 2021-12-07 | 济宁市金益菌生物科技有限公司 | Preparation method of biological deodorant and biological deodorant |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101078003A (en) | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof | |
| CN103910548B (en) | Biological composite fertilizer aqua and production method thereof | |
| CN102924134B (en) | Alga microorganism fertilizer agent and preparation method thereof | |
| CN102219310B (en) | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture | |
| CN103468613B (en) | Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum | |
| CN1757616A (en) | Ferment fungi biological organic fish guano, and its prodn. method | |
| CN101974434B (en) | Multi-bacterium microbial compound preparations for aquaculture and preparation method thereof | |
| CN1911865A (en) | Method of producing active nutritional fertilizer using old domostic garbage | |
| CN101595943A (en) | A kind of production method of additive of bait for photosynthetic bacteria aquaculture | |
| CN108949739A (en) | A kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN1760146A (en) | Nourishing water transfer agent for aquiculture | |
| CN102719380B (en) | Aquatic compound microecologics and preparation method thereof | |
| CN102286376A (en) | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof | |
| CN103013855A (en) | Compound microbial preparation and production process thereof | |
| CN103695336A (en) | Microbial preparation for improving bottom ecological environment of prawn pond and preparation method thereof | |
| CN108813223A (en) | A kind of aquaculture specific complex microorganism formulation and preparation method thereof | |
| CN106854627A (en) | A kind of composite bacteria agent for promoting the flat angle stratiomyiid larval growth of speck and application | |
| CN1190145C (en) | Process for producing stalk protein feed by multi-culture united anaerobic fermentation | |
| CN101078004A (en) | Microorganism preparation for modifying water body by using bacterial and manufacturing method thereof | |
| CN100467584C (en) | Composite microecological agent for aquaculture and scenery water body and its preparation method | |
| CN104651282A (en) | Preparation method of compound photosynthetic bacterial preparation | |
| CN1171539C (en) | Nutrients additive for biological feed of livestock and fowls and its preparing process | |
| CN1271171C (en) | Plant edaphon ecological rehabilitation modifier and method for making same | |
| CN105110489A (en) | Water-purifying and weed-protecting biological agent for shrimp and crab culture in high-temperature period as well as preparation method and application of biological agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20071128 |