CN113750778A - Preparation method of biological deodorant and biological deodorant - Google Patents

Preparation method of biological deodorant and biological deodorant Download PDF

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CN113750778A
CN113750778A CN202111198311.XA CN202111198311A CN113750778A CN 113750778 A CN113750778 A CN 113750778A CN 202111198311 A CN202111198311 A CN 202111198311A CN 113750778 A CN113750778 A CN 113750778A
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fermentation tank
biological deodorant
liquid
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producing
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冯霞
程艳军
潘文文
李兴红
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Jining Jinyizhu Biotechnology Co ltd
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Abstract

The invention provides a preparation method of a biological deodorant and the biological deodorant, and relates to the technical field of deodorants. The preparation method of the biological deodorant is suitable for industrial mass production, is safe and efficient, and can be used for rapidly preparing the biological deodorant and simultaneously ensuring the deodorization effect of the biological deodorant. The biological deodorant can rapidly and thoroughly decompose sulfur-containing and nitrogen-containing organic matters and ammonia gas which generate odor, has acid fragrance after fermentation, plays a role in deodorization, and can be widely used for deodorizing different polluted environments.

Description

Preparation method of biological deodorant and biological deodorant
Technical Field
The invention relates to the technical field of deodorizers, and particularly relates to a preparation method of a biological deodorizer and the biological deodorizer.
Background
Deodorants are mainly of the following types: physical deodorant, chemical deodorant, microbial deodorant, plant deodorant, composite deodorant, etc.
Wherein the physical deodorant is deodorized by a physical method, and only the local concentration or relative concentration of the odor is changed as a result of not changing the odor components by utilizing the physical properties of the deodorant or the odor. Adsorption deodorants, masking deodorants, etc. are common. The chemical deodorant is a deodorant that is produced by converting a generated malodorous substance into a non-malodorous substance by oxidation, reductive decomposition, neutralization reaction, addition reaction, condensation reaction, ion exchange reaction, or the like. The microbial deodorant is a process that malodorous substances dissolved in water are absorbed in the bodies of microbes by the microbes and degraded by the metabolic activity of the microbes. Microbial deodorization can be divided into three stages: firstly, the malodorous gas is dissolved, namely, the malodorous gas is transferred from a gas phase to a liquid phase; secondly, the malodorous components in the water solution are adsorbed and absorbed by microorganisms; thirdly, the malodorous components entering the microbial cells are used as nutrient substances to be decomposed and utilized by the microbes, so that pollutants are removed.
The existing microbial deodorant has a narrow application range and cannot be produced in large batch, so that a biological deodorant is needed to be provided, fecal sewage is efficiently treated, odor is eliminated, the breeding environment is improved, and the pollution to the environment is reduced.
Disclosure of Invention
The invention aims to provide a preparation method of a biological deodorant, which is suitable for industrial mass production, is safe and efficient, and can quickly prepare the biological deodorant and ensure the deodorization effect of the biological deodorant.
The invention also aims to provide a biological deodorant which can rapidly and thoroughly decompose sulfur-containing and nitrogen-containing organic matters and ammonia gas which generate odor, has acid fragrance after fermentation, plays a role in deodorization, and can be widely used for deodorizing different polluted environments.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
In a first aspect, an embodiment of the present application provides a preparation method of a biological deodorant, including the following steps:
and (3) sterilization: opening boiler steam to sterilize the fermentation tank body, and cooling the fermentation tank body after sterilization to obtain a first fermentation tank body and a second fermentation tank body with preset volumes;
preparing a first bacterial liquid: opening a water purifier, adding pure water into the first fermentation tank, adding yeast, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria into the first fermentation tank, sequentially adding peptone, glucose, brown sugar, agar powder, malt extract and sodium chloride, culturing for 24 hours at 36.3-37.2 ℃ to obtain a first preformed product liquid, transferring the first preformed product liquid into the second fermentation tank, culturing for 24 hours at 26-29 ℃, and then putting the second preformed product liquid into a first raw bacteria liquid tank to obtain a first bacteria liquid;
preparing a second bacterial liquid: opening a water purification machine, adding pure water into the first fermentation tank body, adding yeast and lactobacillus into the first fermentation tank body, sequentially adding peptone, glucose, sodium acetate, magnesium sulfate heptahydrate and brown sugar, culturing at 36.3-37.2 ℃ for 24 hours to obtain a second preformed product liquid, transferring the second preformed product liquid into the second fermentation tank body, culturing at 26-29 ℃ for 24 hours, and then putting the second preformed product liquid into a second raw bacterium liquid tank to obtain a second bacterium liquid;
mixing: and (3) mixing the first bacterial liquid and the second bacterial liquid according to the ratio of 1: the mixture is put into the first fermentation tank body according to the proportion of 1, mixed and stirred, and then filled into a ton barrel.
In a second aspect, the present application provides a biological deodorant as described above, which includes yeast, bacillus subtilis, streptomyces microflavus, photosynthetic bacteria and lactobacillus.
In summary, compared with the prior art, the embodiments of the present invention have at least the following advantages or beneficial effects:
the preparation method of the biological deodorant provided by the invention is suitable for industrial mass production, is safe and efficient, can be used for quickly preparing the biological deodorant and simultaneously ensuring the deodorization effect of the biological deodorant, is used for efficiently concentrating the product, and does not need to invest a large amount of equipment.
The biological deodorant provided by the invention is prepared by applying the preparation method of the biological deodorant, and 1) the biological deodorant can effectively remove ammonia nitrogen, hydrogen sulfide, methyl mercaptan, other organic amine organic sulfur, volatile organic acid and other odors in the environment; 2) the product is non-toxic and harmless, does not cause secondary pollution to the environment and animals, and meets the requirement of environmental development; 3) the number of beneficial bacteria in the environment is increased, the breeding of harmful bacteria is reduced, and the environment is beautified; 4) the adaptability is strong: the product is prepared by fermenting a plurality of flora, can adapt to aerobic and anaerobic environments and can adapt to various temperatures and pH values; 5) can be widely used for deodorizing different polluted environments.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In a first aspect, an embodiment of the present application provides a preparation method of a biological deodorant, including the following steps:
and (3) sterilization: opening boiler steam to sterilize the fermentation tank body, and cooling the fermentation tank body after sterilization to obtain a first fermentation tank body and a second fermentation tank body with preset volumes;
preparing a first bacterial liquid: opening a water purifier, adding pure water into the first fermentation tank, adding yeast, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria into the first fermentation tank, sequentially adding peptone, glucose, brown sugar, agar powder, malt extract and sodium chloride, culturing for 24 hours at 36.3-37.2 ℃ to obtain a first preformed product liquid, transferring the first preformed product liquid into the second fermentation tank, culturing for 24 hours at 26-29 ℃, and then putting the second preformed product liquid into a first raw bacteria liquid tank to obtain a first bacteria liquid;
preparing a second bacterial liquid: opening a water purification machine, adding pure water into the first fermentation tank body, adding yeast and lactobacillus into the first fermentation tank body, sequentially adding peptone, glucose, sodium acetate, magnesium sulfate heptahydrate and brown sugar, culturing at 36.3-37.2 ℃ for 24 hours to obtain a second preformed product liquid, transferring the second preformed product liquid into the second fermentation tank body, culturing at 26-29 ℃ for 24 hours, and then putting the second preformed product liquid into a second raw bacterium liquid tank to obtain a second bacterium liquid;
mixing: and (3) putting the first bacterial liquid and the second bacterial liquid into the first fermentation tank according to the proportion of 1:1, mixing and stirring, and filling into a ton barrel after stirring.
In some embodiments of the invention, in the step of preparing the first bacterial liquid:
the mass ratio of the microzyme, the bacillus subtilis and the streptomyces microflavus is 40:360:5, wherein the microzyme is 5 multiplied by 109CFU/g, Bacillus subtilis 2X 1010CFU/g, Streptomyces microflavus 3.6X 1010CFU/g。
In some embodiments of the invention, in the step of preparing the first bacterial liquid:
the mass ratio of peptone, glucose, brown sugar, agar powder, malt extract and sodium chloride is 2000:1000:1000:20:5: 1.
In some embodiments of the invention, in the step of preparing the second bacterial liquid:
the mass ratio of yeast and lactobacillus is 20:24, wherein the yeast is 5 × 109CFU/g, the lactobacillus is 1 × 1011CFU/g。
In some embodiments of the invention, in the step of preparing the second bacterial liquid:
the mass ratio of peptone, glucose, sodium acetate, magnesium sulfate heptahydrate and brown sugar is 60:80:30:6: 60.
In some embodiments of the invention, in the step of preparing the first bacterial liquid:
when the culture is carried out for 24 hours at the temperature of 36.3-37.2 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a first target flora, wherein the first target flora is saccharomycetes, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria.
In some embodiments of the invention, in the step of preparing the second bacterial liquid:
and when the culture is carried out for 24 hours at the temperature of 36.3-37.2 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of the mixed bacteria of a second target flora, wherein the second target flora is saccharomycetes and lactobacillus.
In some embodiments of the invention, in the step of mixing:
and (3) observing and adjusting the number, the production form and the pH value of the bacteria after mixing and stirring, wherein the pH value is 3.2-3.8.
In some embodiments of the invention, in the step of sterilizing:
the sterilization time is 20min, and the preset capacity is 30 tons.
In a second aspect, embodiments of the present application also provide a biological deodorant, which includes yeast, bacillus subtilis, streptomyces microflavus, photosynthetic bacteria, and lactobacillus.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The embodiment aims to provide a preparation method of a biological deodorant, which comprises the following steps:
and (3) sterilization: opening boiler steam to sterilize the fermentation tank body, and cooling the fermentation tank body after sterilization to obtain a first fermentation tank body and a second fermentation tank body with preset volumes;
preparing a first bacterial liquid: opening a water purifier, adding pure water into the first fermentation tank, adding 40kg of yeast, 360kg of bacillus subtilis, 5kg of streptomyces microflavus and 100L of photosynthetic bacteria into the first fermentation tank, sequentially adding 200kg of peptone, 100kg of glucose, 100kg of brown sugar, 2kg of agar powder, 0.5kg of malt extract and 0.1kg of sodium chloride, culturing for 24 hours at 36.3 ℃ to obtain a first preform solution, transferring the first preform solution into the second fermentation tank, culturing for 24 hours at 26 ℃, and then putting into a first raw bacteria solution tank to obtain a first bacteria solution;
preparing a second bacterial liquid: opening a water purification machine, adding pure water into the first fermentation tank body, adding 20kg of yeast and 24kg of lactobacillus into the first fermentation tank body, sequentially adding 80kg of peptone, 60kg of glucose, 30kg of sodium acetate, 6kg of magnesium sulfate heptahydrate and 60kg of brown sugar, culturing for 24 hours at 36.3 ℃ to obtain a second preform liquid, transferring the second preform liquid into the second fermentation tank body, culturing for 24 hours at 26 ℃, and then putting into a second raw bacterium liquid tank to obtain a second bacterium liquid;
mixing: and (3) putting the first bacterial liquid and the second bacterial liquid into the first fermentation tank according to the proportion of 1:1, mixing and stirring, and filling into a ton barrel after stirring.
Wherein the yeast is 5 × 109CFU/g, Bacillus subtilis 2X 1010CFU/g, Streptomyces microflavus 3.6X 1010CFU/g, Bacillus subtilis 1 × 1011CFU/g。
In the step of preparing the first bacterial liquid, when the first bacterial liquid is cultured for 24 hours at 36.3 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a first target flora, wherein the first target flora is saccharomycetes, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria.
In the step of preparing the second bacterial liquid, when the second bacterial liquid is cultured for 24 hours at 36.3 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a second target flora, wherein the second target flora is saccharomycetes and lactobacillus.
In the mixing step, the number, the production form and the pH value of the bacteria are observed and adjusted after mixing and stirring, wherein the pH value is 3.2-3.8.
In the sterilization step, the sterilization time is 20min, and the preset capacity is 30 tons.
The biological deodorant can be produced in large batch with the preset capacity of 30 tons, and the production efficiency is improved.
The biological deodorant is a microbial product prepared by preferential composite fermentation of probiotics, contains various organic acids, bioactive enzymes and bacterial groups, wherein saccharomycetes and lactic acid bacteria decompose organic macromolecules in garbage into micromolecular acids and soluble substances through aerobic and anaerobic fermentation of the microorganisms, inhibit the activities and reproduction of pathogenic bacteria and sclerotinia sclerotiorum, gradually eliminate harmful bacteria, improve the decomposition path of organic matters, reduce the release amount of ammonia and hydrogen sulfide and the generation of ammonia substances, and achieve the effect of deodorization. Can rapidly and thoroughly decompose sulfur-containing and nitrogen-containing organic matters and ammonia gas which generate odor, has acid fragrance after fermentation, and plays a role in deodorization. Is divided into a garbage station deodorant, a farm deodorant, a slaughterhouse deodorant, a sewage deodorant, a toilet deodorant and a microorganism autoclaving deodorant.
The preparation method of the biological deodorant is suitable for industrial mass production, is safe and efficient, can quickly prepare the biological deodorant and simultaneously ensure the deodorization effect of the biological deodorant, is efficient and concentrated, and does not need to invest a large amount of equipment.
Example 2
The embodiment aims to provide a preparation method of a biological deodorant, which comprises the following steps:
and (3) sterilization: opening boiler steam to sterilize the fermentation tank body, and cooling the fermentation tank body after sterilization to obtain a first fermentation tank body and a second fermentation tank body with preset volumes;
preparing a first bacterial liquid: opening a water purifier, adding pure water into the first fermentation tank, adding 40kg of yeast, 360kg of bacillus subtilis, 5kg of streptomyces microflavus and 100L of photosynthetic bacteria into the first fermentation tank, sequentially adding 200kg of peptone, 100kg of glucose, 100kg of brown sugar, 2kg of agar powder, 0.5kg of malt extract and 0.1kg of sodium chloride, culturing at 37.2 ℃ for 24 hours to obtain a first preform solution, transferring the first preform solution into the second fermentation tank, culturing at 29 ℃ for 24 hours, and then putting into a first raw bacteria solution tank to obtain a first bacteria solution;
preparing a second bacterial liquid: opening a water purification machine, adding pure water into the first fermentation tank body, adding 20kg of yeast and 24kg of lactobacillus into the first fermentation tank body, sequentially adding 80kg of peptone, 60kg of glucose, 30kg of sodium acetate, 6kg of magnesium sulfate heptahydrate and 60kg of brown sugar, culturing at 37.2 ℃ for 24 hours to obtain a second preform liquid, transferring the second preform liquid into the second fermentation tank body, culturing at 29 ℃ for 24 hours, and then putting into a second raw bacterium liquid tank to obtain a second bacterium liquid;
mixing: and (3) putting the first bacterial liquid and the second bacterial liquid into the first fermentation tank according to the proportion of 1:1, mixing and stirring, and filling into a ton barrel after stirring.
Wherein the yeast is 5 × 109CFU/g, Bacillus subtilis 2X 1010CFU/g, Streptomyces microflavus 3.6X 1010CFU/g, Bacillus subtilis 1 × 1011CFU/g。
In the step of preparing the first bacterial liquid, when the first bacterial liquid is cultured for 24 hours at 37.2 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a first target flora, wherein the first target flora is saccharomycetes, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria.
In the step of preparing the second bacterial liquid, when the second bacterial liquid is cultured for 24 hours at 37.2 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a second target flora, wherein the second target flora is saccharomycetes and lactobacillus.
In the mixing step, the number, the production form and the pH value of the bacteria are observed and adjusted after mixing and stirring, wherein the pH value is 3.2-3.8.
In the sterilization step, the sterilization time is 20min, and the preset capacity is 30 tons.
The biological deodorant can be produced in large batch with the preset capacity of 30 tons, and the production efficiency is improved.
The biological deodorant is a microbial product prepared by preferential composite fermentation of probiotics, contains various organic acids, bioactive enzymes and bacterial groups, wherein saccharomycetes and lactic acid bacteria decompose organic macromolecules in garbage into micromolecular acids and soluble substances through aerobic and anaerobic fermentation of the microorganisms, inhibit the activities and reproduction of pathogenic bacteria and sclerotinia sclerotiorum, gradually eliminate harmful bacteria, improve the decomposition path of organic matters, reduce the release amount of ammonia and hydrogen sulfide and the generation of ammonia substances, and achieve the effect of deodorization. Can rapidly and thoroughly decompose sulfur-containing and nitrogen-containing organic matters and ammonia gas which generate odor, has acid fragrance after fermentation, and plays a role in deodorization. Is divided into a garbage station deodorant, a farm deodorant, a slaughterhouse deodorant, a sewage deodorant, a toilet deodorant and a microorganism autoclaving deodorant.
The preparation method of the biological deodorant is suitable for industrial mass production, is safe and efficient, can quickly prepare the biological deodorant and simultaneously ensure the deodorization effect of the biological deodorant, is efficient and concentrated, and does not need to invest a large amount of equipment.
Example 3
The embodiment aims to provide a preparation method of a biological deodorant, which comprises the following steps:
and (3) sterilization: opening boiler steam to sterilize the fermentation tank body, and cooling the fermentation tank body after sterilization to obtain a first fermentation tank body and a second fermentation tank body with preset volumes;
preparing a first bacterial liquid: opening a water purification machine, adding pure water into the first fermentation tank body, then adding 40kg of yeast, 360kg of bacillus subtilis, 5kg of streptomyces microflavus and 100L of photosynthetic bacteria into the first fermentation tank body, sequentially adding 200kg of peptone, 100kg of glucose, 100kg of brown sugar, 2kg of agar powder, 0.5kg of malt extract and 0.1kg of sodium chloride, culturing for 24 hours at 36.8 ℃ to obtain a first preform solution, transferring the first preform solution into the second fermentation tank body, culturing for 24 hours at 27.5 ℃, and then placing into a first raw bacteria solution tank to obtain a first bacteria solution;
preparing a second bacterial liquid: opening a water purifier, adding pure water into the first fermentation tank body, adding 20kg of yeast and 24kg of lactobacillus into the first fermentation tank body, sequentially adding 80kg of peptone, 60kg of glucose, 30kg of sodium acetate, 6kg of magnesium sulfate heptahydrate and 60kg of brown sugar, culturing for 24 hours at 36.8 ℃ to obtain a second preformed product liquid, transferring the second preformed product liquid into the second fermentation tank body, culturing for 24 hours at 27.5 ℃, and then putting the second preformed product liquid into a second raw bacteria liquid tank to obtain a second bacteria liquid;
mixing: and (3) putting the first bacterial liquid and the second bacterial liquid into the first fermentation tank according to the proportion of 1:1, mixing and stirring, and filling into a ton barrel after stirring.
Wherein the yeast is 5 × 109CFU/g, Bacillus subtilis 2X 1010CFU/g, Streptomyces microflavus 3.6X 1010CFU/g, Bacillus subtilis 1 × 1011CFU/g。
In the step of preparing the first bacterial liquid, when the first bacterial liquid is cultured for 24 hours at 36.8 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a first target flora, wherein the first target flora is saccharomycetes, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria.
In the step of preparing the second bacterial liquid, when the second bacterial liquid is cultured for 24 hours at 36.8 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a second target flora, wherein the second target flora is saccharomycetes and lactobacillus.
In the mixing step, the number, the production form and the pH value of the bacteria are observed and adjusted after mixing and stirring, wherein the pH value is 3.2-3.8.
In the sterilization step, the sterilization time is 20min, and the preset capacity is 30 tons.
The biological deodorant can be produced in large batch with the preset capacity of 30 tons, and the production efficiency is improved.
The biological deodorant is a microbial product prepared by preferential composite fermentation of probiotics, contains various organic acids, bioactive enzymes and bacterial groups, wherein saccharomycetes and lactic acid bacteria decompose organic macromolecules in garbage into micromolecular acids and soluble substances through aerobic and anaerobic fermentation of the microorganisms, inhibit the activities and reproduction of pathogenic bacteria and sclerotinia sclerotiorum, gradually eliminate harmful bacteria, improve the decomposition path of organic matters, reduce the release amount of ammonia and hydrogen sulfide and the generation of ammonia substances, and achieve the effect of deodorization. Can rapidly and thoroughly decompose sulfur-containing and nitrogen-containing organic matters and ammonia gas which generate odor, has acid fragrance after fermentation, and plays a role in deodorization. Is divided into a garbage station deodorant, a farm deodorant, a slaughterhouse deodorant, a sewage deodorant, a toilet deodorant and a microorganism autoclaving deodorant.
The preparation method of the biological deodorant is suitable for industrial mass production, is safe and efficient, can quickly prepare the biological deodorant and simultaneously ensure the deodorization effect of the biological deodorant, is efficient and concentrated, and does not need to invest a large amount of equipment.
Examples of effects
The purpose of this effect example is to verify the effect of the biological deodorant provided in the foregoing embodiment.
1) Diluting the deodorant by 20 times, and directly spraying the deodorant on the surface of the livestock manure to obtain 100 ML/cubic meter stock solution;
2) the device is matched with the existing spraying and deodorizing equipment for use;
3) washing the ground: deodorizing a colony house: diluting the deodorizer by 10-30 times, and uniformly spraying onto various parts (ground, corners, cages, excrement and urine tanks) of the colony house with a sprayer. Spraying for 2-3 times per week, or spraying once per week after odor is reduced;
4) the septic tank is deodorized (1) the capacity of the prior septic tank is diluted by 10 times according to 1L of deodorant stock solution per cube and then is uniformly sprayed into the tank, and the deodorant stock solution is continuously sprayed every day for 3 days and then is diluted by 10 times according to 100ML of deodorant stock solution per cube and then is sprayed. (2) Deodorizing newly discharged excrement: adding 100ML of deodorant stock solution into each cubic volume of excrement according to the new quantity of excrement collected in the septic tank every day, diluting by 10 times and spraying.
The biological deodorant utilizes the metabolism of microorganism to odor components and biological enzyme to degrade odor components, thereby reducing odor concentration. But wide application in the rubbish transfer station, msw incineration factory, plant, slaughterhouse, food production factory and family bathroom, pet and animal breeding place.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims (10)

1. The preparation method of the biological deodorant is characterized by comprising the following steps:
and (3) sterilization: opening boiler steam to sterilize the fermentation tank body, and cooling the fermentation tank body after sterilization to obtain a first fermentation tank body and a second fermentation tank body with preset volumes;
preparing a first bacterial liquid: opening a water purifier, adding pure water into the first fermentation tank, adding yeast, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria into the first fermentation tank, sequentially adding peptone, glucose, brown sugar, agar powder, malt extract and sodium chloride, culturing for 24 hours at 36.3-37.2 ℃ to obtain a first preformed product liquid, transferring the first preformed product liquid into the second fermentation tank, culturing for 24 hours at 26-29 ℃, and then putting the second preformed product liquid into a first raw bacteria liquid tank to obtain a first bacteria liquid;
preparing a second bacterial liquid: opening a water purification machine, adding pure water into the first fermentation tank body, adding yeast and lactobacillus into the first fermentation tank body, sequentially adding peptone, glucose, sodium acetate, magnesium sulfate heptahydrate and brown sugar, culturing at 36.3-37.2 ℃ for 24 hours to obtain a second preformed product liquid, transferring the second preformed product liquid into the second fermentation tank body, culturing at 26-29 ℃ for 24 hours, and then putting the second preformed product liquid into a second raw bacterium liquid tank to obtain a second bacterium liquid;
mixing: and (3) mixing the first bacterial liquid and the second bacterial liquid according to the ratio of 1: the mixture is put into the first fermentation tank body according to the proportion of 1, mixed and stirred, and then filled into a ton barrel.
2. The method for producing a biological deodorant according to claim 1, wherein in the step of producing the first bacterial liquid:
the mass ratio of the microzyme, the bacillus subtilis and the streptomyces microflavus is 40:360:5, wherein the microzyme is 5 multiplied by 109CFU/g, Bacillus subtilis 2X 1010CFU/g, Streptomyces microflavus 3.6X 1010CFU/g。
3. The method for producing a biological deodorant according to claim 2, wherein in the step of producing the first bacterial liquid:
the mass ratio of peptone, glucose, brown sugar, agar powder, malt extract and sodium chloride is 2000:1000:1000:20:5: 1.
4. The method for producing a biological deodorant according to claim 1, wherein in the step of producing the second bacterial liquid:
the mass ratio of yeast and lactobacillus is 20:24, wherein the yeast is 5 × 109CFU/g, the lactobacillus is 1 × 1011CFU/g。
5. The method for producing a biological deodorant according to claim 4, wherein in the step of producing the second bacterial liquid:
the mass ratio of peptone, glucose, sodium acetate, magnesium sulfate heptahydrate and brown sugar is 60:80:30:6: 60.
6. The method for producing a biological deodorant according to claim 1, wherein in the step of producing the first bacterial liquid:
when the culture is carried out for 24 hours at the temperature of 36.3-37.2 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of mixed bacteria of a first target flora, wherein the first target flora is saccharomycetes, bacillus subtilis, streptomyces microflavus and photosynthetic bacteria.
7. The method for producing a biological deodorant according to claim 6, wherein in the step of producing the second bacterial liquid:
and when the culture is carried out for 24 hours at the temperature of 36.3-37.2 ℃, sampling every 4 hours to detect the quantity, the production form, the pH value and the production condition of the mixed bacteria of a second target flora, wherein the second target flora is saccharomycetes and lactobacillus.
8. The method for preparing a biological deodorant according to claim 7, characterized in that in the mixing step:
and (3) observing and adjusting the number, the production form and the pH value of the bacteria after mixing and stirring, wherein the pH value is 3.2-3.8.
9. The method for preparing a biological deodorant according to claim 1, characterized in that in the step of sterilizing:
the sterilization time is 20min, and the preset capacity is 30 tons.
10. A biological deodorant is characterized in that the biological deodorant comprises yeast, bacillus subtilis, streptomyces microflavus, photosynthetic bacteria and lactobacillus.
CN202111198311.XA 2021-10-14 2021-10-14 Preparation method of biological deodorant and biological deodorant Pending CN113750778A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101078003A (en) * 2006-05-23 2007-11-28 上海创博生态工程有限公司 Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof
CN104962490A (en) * 2015-06-09 2015-10-07 日照益康有机农业科技发展有限公司 Enteromorpha microbial agent and preparation method thereof
CN106047770A (en) * 2016-08-08 2016-10-26 江苏春语生物科技有限公司 Composite liquid microbial agent with long quality guarantee period and preparation method thereof
CN111057662A (en) * 2019-12-04 2020-04-24 鸿坤土壤修复科技(苏州)有限公司 Long-acting quality-guaranteeing compound microbial agent and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101078003A (en) * 2006-05-23 2007-11-28 上海创博生态工程有限公司 Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof
CN104962490A (en) * 2015-06-09 2015-10-07 日照益康有机农业科技发展有限公司 Enteromorpha microbial agent and preparation method thereof
CN106047770A (en) * 2016-08-08 2016-10-26 江苏春语生物科技有限公司 Composite liquid microbial agent with long quality guarantee period and preparation method thereof
CN111057662A (en) * 2019-12-04 2020-04-24 鸿坤土壤修复科技(苏州)有限公司 Long-acting quality-guaranteeing compound microbial agent and preparation method thereof

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