CN112617027A - Bdelloecium bdellovii and preparation method and use method thereof - Google Patents
Bdelloecium bdellovii and preparation method and use method thereof Download PDFInfo
- Publication number
- CN112617027A CN112617027A CN202011638866.7A CN202011638866A CN112617027A CN 112617027 A CN112617027 A CN 112617027A CN 202011638866 A CN202011638866 A CN 202011638866A CN 112617027 A CN112617027 A CN 112617027A
- Authority
- CN
- China
- Prior art keywords
- bacteria
- growth
- water
- leech
- vitamin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 131
- 241000545744 Hirudinea Species 0.000 claims abstract description 116
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 82
- 230000001737 promoting effect Effects 0.000 claims abstract description 39
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims abstract description 34
- 235000013793 astaxanthin Nutrition 0.000 claims abstract description 34
- 229940022405 astaxanthin Drugs 0.000 claims abstract description 34
- 239000001168 astaxanthin Substances 0.000 claims abstract description 34
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims abstract description 34
- 238000002156 mixing Methods 0.000 claims abstract description 23
- 230000009286 beneficial effect Effects 0.000 claims abstract description 19
- 239000000126 substance Substances 0.000 claims abstract description 17
- 230000000975 bioactive effect Effects 0.000 claims abstract description 16
- 235000021323 fish oil Nutrition 0.000 claims abstract description 16
- 241000604931 Bdellovibrio bacteriovorus Species 0.000 claims abstract description 13
- 239000002131 composite material Substances 0.000 claims abstract description 12
- 238000013329 compounding Methods 0.000 claims abstract description 12
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 11
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 11
- 230000036039 immunity Effects 0.000 claims abstract description 11
- 239000000787 lecithin Substances 0.000 claims abstract description 11
- 229940067606 lecithin Drugs 0.000 claims abstract description 11
- 235000010445 lecithin Nutrition 0.000 claims abstract description 11
- 229940088594 vitamin Drugs 0.000 claims abstract description 11
- 229930003231 vitamin Natural products 0.000 claims abstract description 11
- 235000013343 vitamin Nutrition 0.000 claims abstract description 11
- 239000011782 vitamin Substances 0.000 claims abstract description 11
- 241000081271 Phaffia rhodozyma Species 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 8
- 239000007952 growth promoter Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 235000013372 meat Nutrition 0.000 claims description 24
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 23
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 23
- 238000000855 fermentation Methods 0.000 claims description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 210000002826 placenta Anatomy 0.000 claims description 15
- 238000007789 sealing Methods 0.000 claims description 15
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 12
- 241000237858 Gastropoda Species 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 11
- 238000007865 diluting Methods 0.000 claims description 11
- 239000004310 lactic acid Substances 0.000 claims description 11
- 235000014655 lactic acid Nutrition 0.000 claims description 11
- 235000020681 well water Nutrition 0.000 claims description 11
- 239000002349 well water Substances 0.000 claims description 11
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 claims description 10
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 10
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 claims description 10
- 241000233866 Fungi Species 0.000 claims description 10
- 108010055460 bivalirudin Proteins 0.000 claims description 10
- OIRCOABEOLEUMC-GEJPAHFPSA-N bivalirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)CNC(=O)CNC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 OIRCOABEOLEUMC-GEJPAHFPSA-N 0.000 claims description 10
- 229960001500 bivalirudin Drugs 0.000 claims description 10
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 10
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 10
- 229960003512 nicotinic acid Drugs 0.000 claims description 10
- 235000019198 oils Nutrition 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 10
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 10
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 9
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 9
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 9
- 235000017281 sodium acetate Nutrition 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 8
- 230000000243 photosynthetic effect Effects 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 239000003963 antioxidant agent Substances 0.000 claims description 7
- 235000006708 antioxidants Nutrition 0.000 claims description 7
- 241000604933 Bdellovibrio Species 0.000 claims description 6
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 6
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
- 230000003078 antioxidant effect Effects 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 6
- 239000000839 emulsion Substances 0.000 claims description 6
- 239000003205 fragrance Substances 0.000 claims description 6
- 238000000265 homogenisation Methods 0.000 claims description 6
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 108010085443 Anserine Proteins 0.000 claims description 5
- 229920002101 Chitin Polymers 0.000 claims description 5
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 108010024636 Glutathione Proteins 0.000 claims description 5
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 5
- SLRNWACWRVGMKD-UHFFFAOYSA-N L-anserine Natural products CN1C=NC(CC(NC(=O)CCN)C(O)=O)=C1 SLRNWACWRVGMKD-UHFFFAOYSA-N 0.000 claims description 5
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 5
- 241000210053 Potentilla elegans Species 0.000 claims description 5
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 5
- 229930003451 Vitamin B1 Natural products 0.000 claims description 5
- 229930003471 Vitamin B2 Natural products 0.000 claims description 5
- 229930003537 Vitamin B3 Natural products 0.000 claims description 5
- 229930003571 Vitamin B5 Natural products 0.000 claims description 5
- 229930003316 Vitamin D Natural products 0.000 claims description 5
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 5
- 229930003427 Vitamin E Natural products 0.000 claims description 5
- 229930003448 Vitamin K Natural products 0.000 claims description 5
- MYYIAHXIVFADCU-QMMMGPOBSA-N anserine Chemical compound CN1C=NC=C1C[C@H](NC(=O)CC[NH3+])C([O-])=O MYYIAHXIVFADCU-QMMMGPOBSA-N 0.000 claims description 5
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 claims description 5
- 229960002079 calcium pantothenate Drugs 0.000 claims description 5
- 235000009508 confectionery Nutrition 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 239000003995 emulsifying agent Substances 0.000 claims description 5
- 229960000304 folic acid Drugs 0.000 claims description 5
- 235000019152 folic acid Nutrition 0.000 claims description 5
- 239000011724 folic acid Substances 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229960003180 glutathione Drugs 0.000 claims description 5
- 229960000367 inositol Drugs 0.000 claims description 5
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000001471 micro-filtration Methods 0.000 claims description 5
- 235000001968 nicotinic acid Nutrition 0.000 claims description 5
- 239000011664 nicotinic acid Substances 0.000 claims description 5
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 5
- 229940055726 pantothenic acid Drugs 0.000 claims description 5
- 235000019161 pantothenic acid Nutrition 0.000 claims description 5
- 239000011713 pantothenic acid Substances 0.000 claims description 5
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims description 5
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 5
- 229960002477 riboflavin Drugs 0.000 claims description 5
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 229960003080 taurine Drugs 0.000 claims description 5
- 229960003495 thiamine Drugs 0.000 claims description 5
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 5
- 235000010374 vitamin B1 Nutrition 0.000 claims description 5
- 239000011691 vitamin B1 Substances 0.000 claims description 5
- 235000019164 vitamin B2 Nutrition 0.000 claims description 5
- 239000011716 vitamin B2 Substances 0.000 claims description 5
- 235000019160 vitamin B3 Nutrition 0.000 claims description 5
- 239000011708 vitamin B3 Substances 0.000 claims description 5
- 235000009492 vitamin B5 Nutrition 0.000 claims description 5
- 239000011675 vitamin B5 Substances 0.000 claims description 5
- 235000019158 vitamin B6 Nutrition 0.000 claims description 5
- 239000011726 vitamin B6 Substances 0.000 claims description 5
- 235000019166 vitamin D Nutrition 0.000 claims description 5
- 239000011710 vitamin D Substances 0.000 claims description 5
- 150000003710 vitamin D derivatives Chemical class 0.000 claims description 5
- 235000019165 vitamin E Nutrition 0.000 claims description 5
- 229940046009 vitamin E Drugs 0.000 claims description 5
- 239000011709 vitamin E Substances 0.000 claims description 5
- 235000019168 vitamin K Nutrition 0.000 claims description 5
- 239000011712 vitamin K Substances 0.000 claims description 5
- 150000003721 vitamin K derivatives Chemical class 0.000 claims description 5
- 229940045997 vitamin a Drugs 0.000 claims description 5
- 229940011671 vitamin b6 Drugs 0.000 claims description 5
- 229940046008 vitamin d Drugs 0.000 claims description 5
- 229940046010 vitamin k Drugs 0.000 claims description 5
- 241000186361 Actinobacteria <class> Species 0.000 claims description 4
- 239000003910 polypeptide antibiotic agent Substances 0.000 claims description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 2
- 108700042778 Antimicrobial Peptides Proteins 0.000 claims 1
- 102000044503 Antimicrobial Peptides Human genes 0.000 claims 1
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000192700 Cyanobacteria Species 0.000 claims 1
- 102000000521 Immunophilins Human genes 0.000 claims 1
- 108010016648 Immunophilins Proteins 0.000 claims 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims 1
- 241001655322 Streptomycetales Species 0.000 claims 1
- 239000001056 green pigment Substances 0.000 claims 1
- 229910001629 magnesium chloride Inorganic materials 0.000 claims 1
- 230000001546 nitrifying effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 230000029087 digestion Effects 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 210000003608 fece Anatomy 0.000 abstract description 3
- 239000010871 livestock manure Substances 0.000 abstract 2
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 16
- 239000000047 product Substances 0.000 description 14
- 235000017557 sodium bicarbonate Nutrition 0.000 description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 8
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 240000006439 Aspergillus oryzae Species 0.000 description 4
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 4
- 241000108664 Nitrobacteria Species 0.000 description 4
- 241000187759 Streptomyces albus Species 0.000 description 4
- 108010070741 Tachypleus tridentatus tachyplesin peptide Proteins 0.000 description 4
- 235000019270 ammonium chloride Nutrition 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000000091 immunopotentiator Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 241001446247 uncultured actinomycete Species 0.000 description 4
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 3
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000000215 hyperchromic effect Effects 0.000 description 3
- 239000002932 luster Substances 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- ZJQFYZCNRTZAIM-PMXBASNASA-N tachyplesin Chemical compound C([C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@H](C(N[C@H]2CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=3C=CC=CC=3)NC(=O)[C@@H](NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](N)CCCCN)CSSC[C@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC2=O)C(=O)N[C@@H](CCCNC(N)=N)C(N)=O)C(=O)N1)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZJQFYZCNRTZAIM-PMXBASNASA-N 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000019155 vitamin A Nutrition 0.000 description 3
- 239000011719 vitamin A Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 208000028774 intestinal disease Diseases 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000002068 Glycopeptides Human genes 0.000 description 1
- 108010015899 Glycopeptides Proteins 0.000 description 1
- 241000168517 Haematococcus lacustris Species 0.000 description 1
- 108010007267 Hirudins Proteins 0.000 description 1
- 102000007625 Hirudins Human genes 0.000 description 1
- 241000237903 Hirudo Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 101710158668 Placental protein Proteins 0.000 description 1
- 102000008217 Pregnancy Proteins Human genes 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003657 drainage water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229940006607 hirudin Drugs 0.000 description 1
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000003621 irrigation water Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000013586 microbial product Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000009671 shengli Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a leech growth bacterium, a preparation method and a use method thereof. The bdellovibrio bacteriovorus is prepared by compounding 50-70% of growth promoting bacteria and 30-50% of green bacteria; the green bacteria comprise 2.6-2.8% of emulsified astaxanthin, 1.9-2.9% of lecithin, 1.3-2.3% of DHA, 2.5-3.5% of deep sea fish oil, 2.6-3.6% of wall-broken Phaffia rhodozyma, 1.8-2.8% of immunity growth promoter, 6-8% of composite beneficial bacteria, 2-3% of various vitamins, 1-2% of bioactive substances, 1.5-4% of crude protein and 53.5-73.7% of water. The preparation method comprises the steps of preparing the green manure bacteria and the growth promoting bacteria, and mixing and stirring the green manure bacteria and the growth promoting bacteria uniformly according to the formula amount. After dilution, the mixture is used in combination with bait or is splashed in the whole pool. The leech growth bacterium can effectively promote the growth of leeches, enhance the digestion and absorption capacity of leeches on bait, reduce the pollution of leech excrement on water quality, and also has good food calling effect.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and relates to a leech growth bacterium for leech culture, and a preparation method and a use method thereof.
Background
The leech is a traditional special medicinal aquatic animal in China, and the dried product of the leech is soaked in the water and then is used as a medicine in traditional Chinese medicine. With the application and improvement of modern biotechnology, the demand of leeches is increasing year by extraction and processing of hirudin, medical clinical application, and development, development and popularization of Chinese patent medicines. Meanwhile, since numerous leeches are imported from China in Japan, Korea and southeast Asia, the market of domestic leeches is in short supply and the price is increased. Huge market demand, and wide market prospect for artificial breeding of leeches. The development of leech culture has good market prospect and economic benefit.
Leech cultivation has high requirements on water quality, and once a water source is polluted, the leech cultivation has the possibility of 'total military coverage'. In the existing leech culture process, because the leech excrement pollutes water quality, the digestion and absorption capacity of leech on bait is poor, so that the leech grows slowly, and the output rate of the leech is reduced.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a leech growth bacterium which can effectively promote the growth of leeches, enhance the digestion and absorption capacity of leeches on bait and reduce the pollution of leech excrement on water quality, a preparation method and a use method thereof.
The purpose of the invention is realized by the following technical scheme:
the invention relates to a leech growth promoting bacterium, which is prepared by compounding 50-70% of growth promoting bacterium and 30-50% of green bacterium; the green bacteria comprise 2.6-2.8% of emulsified astaxanthin, 1.9-2.9% of lecithin, 1.3-2.3% of DHA, 2.5-3.5% of deep sea fish oil, 2.6-3.6% of wall-broken Phaffia rhodozyma, 1.8-2.8% of immunity growth promoter, 6-8% of composite beneficial bacteria, 2-3% of various vitamins, 1-2% of bioactive substances, 1.5-4% of crude protein and 53.5-73.7% of water.
Further, the immunopotentiator comprises 20% pantothenic acid, 20% inositol, 20% nicotinic acid, 20% folic acid, and 20% amino acids.
Further, the compound beneficial bacteria comprise 20% of EM bacteria, 25% of yeast, 15% of lactic acid bacteria, 20% of bdellovibrio and 20% of nitrobacteria.
Further, the multivitamins include 10% vitamin a, 10% vitamin D, 12% vitamin E, 10% vitamin K, 12% vitamin B1, 12% vitamin B2, 13% vitamin B3, 11% vitamin B5, and 10% vitamin B6.
Further, the bioactive substances comprise 15% taurine, 15% EPA, 15% PHA, 15% glutathione, 15% anserine, 12% tachyplesin antimicrobial peptide and 13% chitin.
Furthermore, the bdellovibrio bacteriovorus is prepared by compounding 60% of growth promoting bacteria and 40% of green bacteria.
The percentages stated in the invention are mass percentages.
The growth promoting bacteria of the invention are five effective strains in five types of microbial floras of photosynthetic bacteria, lactic acid bacteria, saccharomycetes, actinomycetes and fungi, through respective domestication, rejuvenation and culture, by utilizing the characteristic that beneficial substances produced by the microbial floras in the respective growth processes can mutually serve as growth substrates and form symbiotic proliferation relationship, a proper flora structure is selected, pig placental protein containing natural growth promoting factors is used as a culture medium to ferment and expand the floras, and finally, a compound probiotic growth promoting microbial product with complex flora, complete functions and stable structure is formed, so that the synergistic effect of various strains is realized, the effect of the natural growth promoting factors is effectively exerted, the intestinal tract of animals is balanced, the growth of animal organisms is promoted, the culture ecological environment is optimized, and the growth promoting bacteria are safe, stable and pollution-free, A pollution-free high-efficiency green environment-friendly feed additive.
Further, the growth promoting bacteria are prepared by the following preparation method:
firstly, taking a proper amount of pig placenta, quickly freezing, cutting into small diced meat, and mincing into uniform meat pulp to obtain the pig placenta meat pulp for later use.
② 0.5-1 g of ammonia chloride (NH4Cl), 0.75-1.25 g of sodium bicarbonate (NaHCO3), 0.25-0.75 g of dipotassium hydrogen phosphate (K2HPO4), 1.5-2 g of sodium acetate (CH3COONa), 0.3-0.5 g of magnesium chloride (MgCl2), 1.5-2.5 g of sodium chloride (NaCl) and 8-12 g of glucose (C6H12O6) are added into every 1L of distilled water to prepare a culture solution for later use.
③ taking the photosynthetic bacteria (Rhodopseudomonas palustris (P. rhodopseudomonas palustris) with the strain content of 30 multiplied by 108A lactic acid bacteria (Lactobacillus bulgaricus) content of 5 × 108The content of yeast (Saccharomyces cerevisiae) is 20 × 108The content of fungus (Aspergillus oryzae) strain is 5 × 106The content of actinomycete (Streptomyces albus) is 5 × 106And/ml or more, and compounding according to the volume ratio of 55-65:15-25:5-15:3-6:3-6 to prepare the strain for later use.
And thirdly, pouring the strain prepared in the third step and the culture solution prepared in the second step into a fermentation tank according to the volume ratio of 1:3-5, sealing, keeping the pH value at 4.0-5.0 and the temperature at 23-28 ℃, performing anaerobic fermentation for 4-10 days, and preparing a bacterial solution for later use when the material emits slightly sweet and sour strong distiller's yeast fragrance, which indicates that the fermentation is successful.
Fifthly, uniformly mixing the pig placenta meat slurry obtained in the step I and the bacterial liquid obtained in the step II according to the weight ratio of 1:2-4, pouring the mixture into a fermentation tank, sealing, keeping the pH value at 4.0-5.0 and the temperature at 23-28 ℃, and performing anaerobic fermentation culture for 4-10 days to obtain the growth-promoting bacteria.
The preparation method of the bdellovibrio bacteriovorus comprises the following steps:
the method comprises the following steps:
s1, preparing emulsified astaxanthin; the preparation method of the emulsified astaxanthin comprises the following steps:
(1) dissolving an emulsifier and an antioxidant in deionized water, and fully mixing to obtain a water phase;
(2) uniformly mixing fish oil and astaxanthin to obtain an oil phase;
(3) respectively pouring the water phase obtained in the step (1) and the oil phase obtained in the step (2) into two feed inlets of a dual-channel micro-jet device for homogenization;
(4) and (4) carrying out microfiltration, light protection and sealing treatment on the emulsion after circulating and homogenizing for 1 time to obtain the emulsified astaxanthin.
S2: and then the emulsified astaxanthin prepared by the S1 is mixed with lecithin, DHA, deep sea fish oil, wall-broken phaffia rhodozyma, immunity growth promoting elements, composite beneficial bacteria, a plurality of vitamins, bioactive substances, crude protein and water according to the formula amount and stirred evenly to prepare the Qingsu bacteria.
And (II) mixing and stirring the growth promoting bacteria and the biotin bacteria uniformly according to the formula amount to obtain the hirulog bacteria.
The method for using the leech growth bacteria comprises the following steps: spraying the diluted leech bait into bait for matching with the bait in each stage of leech cultivation, or spraying the diluted leech bait into a whole pond after being diluted by cultivation water; the dosage is as follows:
1) feeding with bait: diluting 150 ml of the leech growth bacteria with 250 ml of well water (or purified water) by 2 kg, and directly spraying 100 kg of bait snails for feeding;
2) promoting appetite and attracting food: diluting 150 ml of the leech growth bacteria with 250 ml of well water (or purified water) by 1 kg of well water, and directly spraying 50 kg of bait snails for feeding;
3) splashing of the leech growth bacterium water body: 50-100 ml of the leech growth bacteria is diluted by 50-100 times of culture water and directly sprinkled once every 5-8 days in each mu of pond (1 m of water depth).
The invention has the beneficial effects that:
the invention relates to a leech growth-promoting microbe, which is characterized in that more than ten effective strains in five microbial floras of photosynthetic bacteria, lactic acid bacteria, wall-broken phaffia rhodozyma, actinomycetes, filamentous bacteria and the like separated from intestinal tracts of healthy aquatic animals, haematococcus pluvialis and high-yield culture water are domesticated, rejuvenated and cultured respectively, the characteristics that beneficial substances generated in the respective growth processes of the microbial floras can be mutually used as growth substrates and form symbiotic proliferation relationship are utilized, a proper flora structure is selected, animal protoembryonic protein containing natural growth promoting factors and an antioxidant are used as culture media to ferment and expand the floras, and trace elements, vitamins and nano growth promoting substances necessary for leech growth are compounded at the same time, and finally, a compound probiotic growth-promoting microbe product with complex flora, complete functions and stable structure is formed.
The inventive leech growth bacterium can effectively promote the growth of leeches, enhance the digestion and absorption capacity of leeches to bait, reduce the bait coefficient, reduce the pollution of leech feces to water quality, and has good food calling effect.
The invention relates to a leech growth bacterium which is a new-generation microecological preparation product developed by utilizing natural animal and plant antioxidants and nano growth promoting factors according to the theory of animal nutrition, does not contain chemical hormones, and is a necessary product for producing non-resistance green leeches.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
The embodiment relates to a leech growth-promoting bacterium which is prepared by compounding 60 percent of growth-promoting bacterium and 40 percent of green bacterium;
the said fungus, Hongbaoqingsu, includes 2.7% emulsified astaxanthin, 2.4% lecithin, 1.8% DHA, 3% deep sea fish oil, 3.1% wall-broken red hair yeast, 2.3% immunity growth promoter, 7% composite beneficial bacteria, 2.5% multivitamins, 1.5% bioactive matter, 2.75% crude protein and 70.95% water.
The immunopotentiator comprises 20% pantothenic acid, 20% inositol, 20% nicotinic acid, 20% folic acid and 20% amino acid.
The compound beneficial bacteria comprise 20% of EM bacteria, 25% of yeast, 15% of lactic acid bacteria, 20% of bdellovibrio and 20% of nitrobacteria.
The multivitamins include 10% vitamin A, 10% vitamin D, 12% vitamin E, 10% vitamin K, 12% vitamin B1, 12% vitamin B2, 13% vitamin B3, 11% vitamin B5, and 10% vitamin B6.
The bioactive substances comprise 15% taurine, 15% EPA, 15% PHA, 15% glutathione, 15% anserine, 12% tachyplesin antibacterial peptide and 13% chitin.
The growth promoting bacterium is Hongbao growth promoting bacterium, and is prepared by the following preparation method:
taking 500g of pig placenta, quickly freezing at the temperature of-20 ℃, cutting into small diced meat with the diameter of 1.5cm multiplied by 1.5cm, and placing the diced meat into a meat grinder for homogenate to obtain pig placenta meat pulp for later use.
② 0.75 g of ammonia chloride (NH4Cl), 1 g of sodium bicarbonate (NaHCO3), 0.5 g of dipotassium hydrogen phosphate (K2HPO4), 1.75 g of sodium acetate (CH3COONa), 0.4 g of magnesium chloride (MgCl2), 2 g of sodium chloride (NaCl) and 10 g of glucose (C6H12O6) are added with distilled water and added to 1000 ml to prepare a culture solution for later use.
③ taking the photosynthetic bacteria (Rhodopseudomonas palustris (P. rhodopseudomonas palustris) with the strain content of 30 multiplied by 108A lactic acid bacteria (Lactobacillus bulgaricus) content of 5 × 108The content of yeast (Saccharomyces cerevisiae) is 20 × 108The content of fungus (Aspergillus oryzae) strain is 5 × 106The content of actinomycete (Streptomyces albus) is 5 × 106And/ml or more, and compounding according to the volume ratio of 60:20:10:5:5 to prepare the strain for later use.
And fourthly, pouring the strain prepared in the third step and the culture solution prepared in the second step into a fermentation tank according to the volume ratio of 1:4, sealing, keeping the pH value at 4.0-5.0 and the temperature at 23-28 ℃, performing anaerobic fermentation for 4-10 days, and obtaining a bacterial solution for later use after the materials emit slightly sweet and sour strong distiller's yeast fragrance.
And fourthly, uniformly mixing the pig placenta meat slurry obtained in the step IV and the bacterial liquid obtained in the step IV according to the weight ratio of 1:3, pouring the mixture into a fermentation tank, sealing, keeping the pH value at 4.0-5.0 and the temperature at 23-28 ℃, and carrying out anaerobic fermentation culture for 4-10 days to obtain the growth-promoting bacteria.
The preparation method of the bdellovibrio bacteriovorus comprises the following steps:
the method comprises the following steps:
s1, preparing emulsified astaxanthin; the preparation method of the emulsified astaxanthin comprises the following steps:
(1) dissolving an emulsifier and an antioxidant in deionized water, and fully mixing to obtain a water phase;
(2) uniformly mixing fish oil and astaxanthin to obtain an oil phase;
(3) respectively pouring the water phase obtained in the step (1) and the oil phase obtained in the step (2) into two feed inlets of a dual-channel micro-jet device for homogenization;
(4) and (4) carrying out microfiltration, light protection and sealing treatment on the emulsion after circulating and homogenizing for 1 time to obtain the emulsified astaxanthin.
S2: and then the emulsified astaxanthin prepared by the S1 is mixed with lecithin, DHA, deep sea fish oil, wall-broken phaffia rhodozyma, immunity growth promoting elements, composite beneficial bacteria, a plurality of vitamins, bioactive substances, crude protein and water according to the formula amount and stirred evenly to prepare the Qingsu bacteria.
And (II) mixing and stirring the growth promoting bacteria and the biotin bacteria uniformly according to the formula amount to obtain the hirulog bacteria.
The method for using the hirulog of the embodiment is as follows: after being diluted, the water is sprayed into bait for matching with the bait or is sprayed into the whole pond after being diluted by culture water.
[ application scope ] is suitable for each stage of leech cultivation.
(dosage) 1, feeding with bait: diluting 200 ml of the feed with 2 kg of well water (or purified water), and directly spraying 100 kg of bait snails for feeding;
2. appetizing and attracting food: diluting 200 ml of the feed with 1 kg of well water (or purified water), and directly spraying 50 kg of bait snails for feeding;
3. splashing water: 100 ml of the product is directly sprayed after being diluted by 50 times with culture water every mu of pond (1 m in water depth), and the product is sprayed once every 7 days.
Example 2
The embodiment relates to a leech growth-promoting bacterium which is prepared by compounding 50 percent of growth-promoting bacterium and 50 percent of green bacterium;
the said fungus, Hongbaoqingsu, includes 2.6% emulsified astaxanthin, 2.9% lecithin, 2.3% DHA, 2.5% deep sea fish oil, 2.6% wall-broken red hair yeast, 2.8% immunity growth promoter, 8% composite beneficial bacteria, 3% multivitamins, 1% bioactive matter, 4% crude protein and 68.3% water.
The immunopotentiator comprises 20% pantothenic acid, 20% inositol, 20% nicotinic acid, 20% folic acid and 20% amino acid.
The compound beneficial bacteria comprise 20% of EM bacteria, 25% of yeast, 15% of lactic acid bacteria, 20% of bdellovibrio and 20% of nitrobacteria.
The multivitamins include 10% vitamin A, 10% vitamin D, 12% vitamin E, 10% vitamin K, 12% vitamin B1, 12% vitamin B2, 13% vitamin B3, 11% vitamin B5, and 10% vitamin B6.
The bioactive substances comprise 15% taurine, 15% EPA, 15% PHA, 15% glutathione, 15% anserine, 12% tachyplesin antibacterial peptide and 13% chitin.
The growth promoting bacterium is Hongbao growth promoting bacterium, and is prepared by the following preparation method:
taking 500g of pig placenta, quickly freezing at-20 ℃, cutting into small diced meat with the diameter of 1.5cm multiplied by 1.5cm, and placing the diced meat into a meat grinder for homogenate to obtain pig placenta meat pulp for later use.
Sixthly, 0.5 g of ammonia chloride (NH4Cl), 0.75 g of sodium bicarbonate (NaHCO3), 0.25 g of dipotassium phosphate (K2HPO4), 1.5 g of sodium acetate (CH3COONa), 0.3 g of magnesium chloride (MgCl2), 1.5 g of sodium chloride (NaCl) and 8 g of glucose (C6H12O6) are taken, distilled water is added into the mixture, and the mixture is added to 1000 ml to prepare a culture solution for later use.
The content of photosynthetic bacteria (rhodopseudomonas palustris) strains is 30 multiplied by 108A lactic acid bacteria (Lactobacillus bulgaricus) content of 5 × 108The content of yeast (Saccharomyces cerevisiae) is 20 × 108The content of fungus (Aspergillus oryzae) strain is 5 × 106The content of actinomycete (Streptomyces albus) is 5 × 106And/ml or more, and compounding according to the volume ratio of 55:15:5:3:3 to obtain the strain for later use.
And fourthly, pouring the strain prepared in the third step and the culture solution prepared in the second step into a fermentation tank according to the volume ratio of 1:5, sealing, keeping the pH value at 4.0 and the temperature at 28 ℃, performing anaerobic fermentation for 4 days, and obtaining a bacterial solution for later use when the materials emit slightly sweet and sour strong distiller's yeast fragrance.
Eighthly, uniformly mixing the pig placenta meat slurry obtained in the step I and the bacterial liquid obtained in the step II according to the weight ratio of 1:4, pouring the mixture into a fermentation tank, sealing, keeping the pH value at 4.0 and the temperature at 28 ℃, and carrying out anaerobic fermentation culture for 4 days to obtain the growth-promoting bacteria.
The preparation method of the bdellovibrio bacteriovorus comprises the following steps:
the method comprises the following steps:
s1, preparing emulsified astaxanthin; the preparation method of the emulsified astaxanthin comprises the following steps:
(1) dissolving an emulsifier and an antioxidant in deionized water, and fully mixing to obtain a water phase;
(2) uniformly mixing fish oil and astaxanthin to obtain an oil phase;
(3) respectively pouring the water phase obtained in the step (1) and the oil phase obtained in the step (2) into two feed inlets of a dual-channel micro-jet device for homogenization;
(4) and (4) carrying out microfiltration, light protection and sealing treatment on the emulsion after circulating and homogenizing for 1 time to obtain the emulsified astaxanthin.
S2: and then the emulsified astaxanthin prepared by the S1 is mixed with lecithin, DHA, deep sea fish oil, wall-broken phaffia rhodozyma, immunity growth promoting elements, composite beneficial bacteria, a plurality of vitamins, bioactive substances, crude protein and water according to the formula amount and stirred evenly to prepare the Qingsu bacteria.
And (II) mixing and stirring the growth promoting bacteria and the biotin bacteria uniformly according to the formula amount to obtain the hirulog bacteria.
The method for using the hirulog of the embodiment is as follows: after being diluted, the water is sprayed into bait for matching with the bait or is sprayed into the whole pond after being diluted by culture water.
[ application scope ] is suitable for each stage of leech cultivation.
(dosage) 1, feeding with bait: diluting 150 ml of the feed with 2 kg of well water (or purified water), and directly spraying 100 kg of bait snails for feeding;
2. appetizing and attracting food: diluting 150 ml of the feed with 1 kg of well water (or purified water), and directly spraying 50 kg of bait snails for feeding;
3. splashing water: 50 ml of the product is directly sprayed after being diluted by 100 times with culture water every mu of pond (1 m in water depth), and the product is sprayed once every 5 days.
Example 3
The embodiment relates to a leech growth-promoting bacterium, which is prepared by compounding 70 percent of growth-promoting bacterium and 30 percent of green bacterium;
the said fungus, Hongbaoqingsu, includes 2.8% emulsified astaxanthin, 1.9% lecithin, 1.3% DHA, 3.5% deep sea fish oil, 23.6% wall-broken red hair yeast, 1.8% immunity growth promoter, 6% composite beneficial bacteria, 2% multivitamins, 2% bioactive matter, 1.5% crude protein and 53.6% water.
The immunopotentiator comprises 20% pantothenic acid, 20% inositol, 20% nicotinic acid, 20% folic acid and 20% amino acid.
The compound beneficial bacteria comprise 20% of EM bacteria, 25% of yeast, 15% of lactic acid bacteria, 20% of bdellovibrio and 20% of nitrobacteria.
The multivitamins include 10% vitamin A, 10% vitamin D, 12% vitamin E, 10% vitamin K, 12% vitamin B1, 12% vitamin B2, 13% vitamin B3, 11% vitamin B5, and 10% vitamin B6.
The bioactive substances comprise 15% taurine, 15% EPA, 15% PHA, 15% glutathione, 15% anserine, 12% tachyplesin antibacterial peptide and 13% chitin.
The growth promoting bacterium is Hongbao growth promoting bacterium, and is prepared by the following preparation method:
ninthly, taking 500g of pig placenta, quickly freezing at the temperature of minus 20 ℃, cutting into small diced meat with the size of 1.5cm multiplied by 1.5cm, and placing the diced meat into a meat grinder for homogenate to obtain pig placenta meat pulp for later use.
(r) 1 g of ammonia chloride (NH4Cl), 1.25 g of sodium bicarbonate (NaHCO3), 0.75 g of dipotassium hydrogen phosphate (K2HPO4), 2 g of sodium acetate (CH3COONa), 0.5 g of magnesium chloride (MgCl2), 2.5 g of sodium chloride (NaCl) and 12 g of glucose (C6H12O6) are taken, distilled water is added into the mixture, and the mixture is added to 1000 ml to prepare a culture solution for later use.
And fourthly, pouring the strain prepared in the third step and the culture solution prepared in the second step into a fermentation tank according to the volume ratio of 1:3, sealing, keeping the pH value at 5.0 and the temperature at 23 ℃, performing anaerobic fermentation for 10 days, and obtaining a bacterial solution for later use when the materials emit slightly sweet and sour strong distiller's yeast fragrance.
The preparation method of the bdellovibrio bacteriovorus comprises the following steps:
the method comprises the following steps:
s1, preparing emulsified astaxanthin; the preparation method of the emulsified astaxanthin comprises the following steps:
(1) dissolving an emulsifier and an antioxidant in deionized water, and fully mixing to obtain a water phase;
(2) uniformly mixing fish oil and astaxanthin to obtain an oil phase;
(3) respectively pouring the water phase obtained in the step (1) and the oil phase obtained in the step (2) into two feed inlets of a dual-channel micro-jet device for homogenization;
(4) and (4) carrying out microfiltration, light protection and sealing treatment on the emulsion after circulating and homogenizing for 1 time to obtain the emulsified astaxanthin.
S2: and then the emulsified astaxanthin prepared by the S1 is mixed with lecithin, DHA, deep sea fish oil, wall-broken phaffia rhodozyma, immunity growth promoting elements, composite beneficial bacteria, a plurality of vitamins, bioactive substances, crude protein and water according to the formula amount and stirred evenly to prepare the Qingsu bacteria.
And (II) preparing the growth promoting bacteria according to the preparation method of the growth promoting bacteria.
And (III) mixing and stirring the bacterial strain of the green hormone and the growth promoting bacteria uniformly according to the formula amount to prepare the leech growth bacteria.
The method for using the hirulog of the embodiment is as follows: after being diluted, the water is sprayed into bait for matching with the bait or is sprayed into the whole pond after being diluted by culture water.
[ application scope ] is suitable for each stage of leech cultivation.
(dosage) 1, feeding with bait: diluting 250 ml of the feed with 2 kg of well water (or purified water), and directly spraying 100 kg of bait snails for feeding;
2. appetizing and attracting food: diluting 250 ml of the feed with 1 kg of well water (or purified water), and directly spraying 50 kg of bait snails for feeding;
3. splashing water: 75 ml of the product is diluted by 75 times of culture water and directly sprayed in each mu of pond (1 m in water depth), and the product is sprayed once every 8 days.
The efficacy, main ingredients, and use notes of the hirulog products prepared in examples 1 to 3 are as follows:
[ EFFECT ]
1. Contains amino acids, polypeptide, multiple vitamins, natural growth promoting factor and nanometer growth promoting factor, and can promote rapid growth of Hirudo and increase weight gain rate;
2. contains trace elements and astaxanthin, maintains the nutrient balance of bait, promotes the healthy development of leeches, has super strong natural hyperchromic effect, can improve the natural body color of leeches and improve the commodity value of the leeches;
3. the organic acid can improve the digestion and utilization rate of the leech on bait and reduce the culture cost;
4. the leech intestinal flora balance is maintained, the incidence rate of intestinal diseases is reduced, and the pollution of leech excrement organic matters to water quality is reduced;
5. the leech growth bacteria containing natural astaxanthin can improve the anti-strain capacity of leeches, repair damaged cells and tissues, and effectively improve the anti-variation capacity of leeches when used before sudden change of weather;
6. has the advantages of fermentation fragrance and good food calling effect.
[ physicochemical Properties ] brownish red viscous emulsion.
[ MAIN COMPONENTS ] photosynthetic bacteria, lactobacillus, wall-broken Phaffia rhodozyma, actinomycetes, filamentous fungi, trace elements, amino acids, various vitamins, astaxanthin, natural growth promoting factors, nano growth promoting factors, glycopeptides, etc.
[ active ingredient content ] 20 × 108 probiotics per gram.
[ Note ] 1, it cannot be used when leech has intestinal diseases or is fed with antibiotics;
2. the product has no high temperature resistance, and can be used for diluting water with temperature lower than 40 deg.C;
3. the product is not affected by water temperature and illumination, and can ensure good water quality after being used frequently;
4. the product has better using effect when being matched with 'Hongbao shengli bacteria' and 'Hongbao EM bacteria'.
[ PACKAGE ] 200 ml/bottle 30 bottles/box.
[ STORAGE ] the storage effect is best in a shady and cool, dry, dark and ventilated place, and the storage effect in a refrigerator cold room (5-10 ℃).
[ shelf life ] for 12 months
Application examples
Use case of leech growth bacteria in leech culture process
Treating a water body: two adjacent leech culture ponds with the water surface of 1 mu are taken for comparative tests in leech culture ponds with the water surface of 1 mu in adjacent fish platforms in Shandong Jining City, counties of Li loft Tegong and village, the water depth is about 1 m, the ponds are in the south-north direction, and are rectangular, and the drainage and irrigation water is convenient. The leech seed throwing time is 7 months and 20 days in 2020, the leech seed stocking specification is 1 cm in body length and 5 ten thousand per kilogram, the leech seed stocking is high in activity, smooth in body surface, bright in color, free of damage and pathology, and the leech seed stocking density is as follows: 15 ten thousand per mu, divided into an experimental group and a control group, and the treatment period is 3 months.
Problem analysis: during the experiment, after the leech growth bacteria are sprinkled and fed in the whole pond in a mixing manner with the snail bait, the contrast influence on the survival rate, the weight gain rate, the body color and the commodity purchase price of the leech is exerted.
The treatment scheme is as follows:
experimental groups: 100 ml of the leech growth bacteria of the embodiment 1 and a proper amount of culture water (diluted by 50 times) are uniformly mixed on each mu of water surface (the depth of water is 1 m) and then are uniformly sprinkled once every 7 days.
Control group: no bdellovibrio bacteriovorus is used.
The control group and the experimental group are fed with baits once a week, and other feeding conditions and feeding amount are the same.
The results were followed after treatment, and the number of samples taken per time was the average weight obtained for 100 leech larvae.
Observation dates are 7 months, 20 days-8 months, 19 days-8 months, 20 days-9 months, 19 days-9 months, 20 days-10 months, 19 days
Control group: firstly, about 6 kg of dead leeches are found in the pond inspection, the average weight of the sampled live leeches is 0.4 g/strip, and the number of the dead leeches is 15000. ② about 30 kg of dead leeches are found in pond patrol, the average weight of the sampled live leeches is 1.5 g/strip, and the number of the dead leeches is 20000. ③ about 212.5 kg of dead leeches are found in the pond patrol, the average weight of the sampled live leeches is 8.5 g/one leech, and the number of the dead leeches is 25000. Remarking: the total number of dead leeches is 60000, and the survival rate is 60%.
Commercial phase and dry body acquisition description: part of the leeches are blackish in appearance and color, and the natural black luster of the leeches is lost. The dried leeches are crisp when broken, the leeches have tongue-killing feeling after being astringent when tasted, the acquisition market feeds back and sells the leeches generally, and the highest acquisition price is 1000 yuan/kg.
Experimental groups: firstly, about 6 kg of dead leeches are found in pond patrol, the average weight of the sampled live leeches is 0.5 g/strip, and the number of the dead leeches is 12000. ② about 25 kg of dead leeches are found in pond patrol, the average weight of the sampled live leeches is 2.5 g/strip, and the number of dead leeches is 10000. ③ about 50 kg of dead leeches are found in the pond patrol, the average weight of the sampled live leeches is 10 g/piece, and the number of the dead leeches is 5000. Remarking: 27000 dead leeches and 82 percent survival rate.
Commercial phase and dry body acquisition description: the leeches have natural black luster on the back of the appearance, have toughness when being broken, have colloid-like luster on the cross section, have light taste and fishy smell, and have elastic meat quality when being touched by hands; is deeply favored by the purchasing market, and the maximum purchasing price of the dried leech can reach 1400 yuan/kg.
Wherein, the survival rate is (the total number of the thrown seedlings-the total number of dead leeches)/the total number of the thrown seedlings.
Case analysis:
through observation of the whole experimental process, the following conclusions can be drawn:
(I) the growth speed of leeches is obviously accelerated
During the experiment period, the growth speed of the experimental group leech is obviously accelerated compared with that of the control group, and the average weight of the experimental group leech is finally increased by 15 percent compared with that of the control group.
(II) obviously improving the survival rate of leeches
During the experiment period, the survival rate of the leech is improved by 22 percent compared with that of the control group after the experimental group is added with the leech growth bacteria.
(III) has natural hyperchromic effect and can improve commodity value
The leech growth bacterium has super strong natural hyperchromic effect on leeches, can improve the natural body color of the leeches, and improves the commodity value of the leeches.
Claims (10)
1. A bdellovibrio bacteriovorus is characterized in that the bdellovibrio bacteriovorus is prepared by compounding 50-70% of growth promoting bacteria and 30-50% of green bacteria; the green bacteria comprise 2.6-2.8% of emulsified astaxanthin, 1.9-2.9% of lecithin, 1.3-2.3% of DHA, 2.5-3.5% of deep sea fish oil, 2.6-3.6% of wall-broken Phaffia rhodozyma, 1.8-2.8% of immunity growth promoter, 6-8% of composite beneficial bacteria, 2-3% of various vitamins, 1-2% of bioactive substances, 1.5-4% of crude protein and 53.5-73.7% of water.
2. The bdellovibrio bacteriovorus according to claim 1, wherein the bdellovibrio bacteriovorus is prepared by mixing 60% of growth-promoting bacteria and 40% of cyanobacteria.
3. The bacterium of claim 1 or 2, wherein the immunophilin comprises 20% pantothenic acid, 20% inositol, 20% niacin, 20% folic acid, 20% amino acids.
4. The bdellovibrio bacteriovorus according to claim 1 or 2, wherein the composite beneficial bacteria comprise 20% of EM bacteria, 25% of yeast, 15% of lactic acid bacteria, 20% of bdellovibrio bacteriovorus and 20% of nitrifying bacteria.
5. The hirulog according to claim 1 or 2, wherein the multivitamins comprise 10% vitamin a, 10% vitamin D, 12% vitamin E, 10% vitamin K, 12% vitamin B1, 12% vitamin B2, 13% vitamin B3, 11% vitamin B5, 10% vitamin B6.
6. The bdellovibrio according to claim 1 or 2, wherein the bioactive substance comprises 15% taurine, 15% EPA, 15% PHA, 15% glutathione, 15% anserine, 12% tachyplesin-like antimicrobial peptide, 13% chitin.
7. The bdellovibrio according to claim 1 or 2, wherein the growth-promoting bacteria are prepared by the following method:
taking a proper amount of pig placenta, quickly freezing, cutting into small diced meat, and mincing into uniform meat pulp to obtain pig placenta meat pulp for later use;
② 0.5-1 g of ammonia chloride, 0.75-1.25 g of sodium bicarbonate, 0.25-0.75 g of dipotassium phosphate, 1.5-2 g of sodium acetate, 0.3-0.5 g of magnesium chloride, 1.5-2.5 g of sodium chloride and 8-12 g of glucose are added into every 1 liter of distilled water to prepare culture solution for standby;
③ taking the content of the rhodopseudomonas palustris strain in the photosynthetic bacteria to be 30 multiplied by 108A lactobacillus bulgaricus strain content of 5 × 10 in the lactic acid bacteria8Per ml or above, the content of Saccharomyces cerevisiae strain in the yeast is 20 × 108/ml or more, the content of Aspergillus species in the fungus is 5 × 106White color in actinomycetes/ml or moreThe streptomycete strain content is 5 multiplied by 106Or more than one, and compounding according to the volume ratio of 55-65:15-25:5-15:3-6:3-6 to prepare strains for later use;
thirdly, pouring the strain prepared in the third step and the culture solution prepared in the second step into a fermentation tank according to the volume ratio of 1:3-5, sealing, keeping the pH value at 4.0-5.0 and the temperature at 23-28 ℃, performing anaerobic fermentation for 4-10 days, and preparing a bacterial solution for later use when the materials emit slightly sweet and sour strong distiller's yeast fragrance, which indicates that the fermentation is successful;
fifthly, uniformly mixing the pig placenta meat slurry obtained in the step I and the bacterial liquid obtained in the step II according to the weight ratio of 1:2-4, pouring the mixture into a fermentation tank, sealing, keeping the pH value at 4.0-5.0 and the temperature at 23-28 ℃, and performing anaerobic fermentation culture for 4-10 days to obtain the growth-promoting bacteria.
8. A process for the preparation of the bacterium hirudinium according to any one of claims 1 to 7, comprising the following steps:
the method comprises the following steps:
s1, preparing emulsified astaxanthin; the preparation method of the emulsified astaxanthin comprises the following steps:
(1) dissolving an emulsifier and an antioxidant in deionized water, and fully mixing to obtain a water phase;
(2) uniformly mixing fish oil and astaxanthin to obtain an oil phase;
(3) respectively pouring the water phase obtained in the step (1) and the oil phase obtained in the step (2) into two feed inlets of a dual-channel micro-jet device for homogenization;
(4) and (4) carrying out microfiltration, light protection and sealing treatment on the emulsion after circulating homogenization to obtain the emulsified astaxanthin.
S2: and then the emulsified astaxanthin prepared by the S1 is mixed with lecithin, DHA, deep sea fish oil, wall-broken phaffia rhodozyma, immunity growth promoting elements, composite beneficial bacteria, a plurality of vitamins, bioactive substances, crude protein and water according to the formula amount and stirred evenly to prepare the Qingsu bacteria.
And (II) mixing and stirring the growth promoting bacteria and the green pigment bacteria uniformly according to the formula amount to obtain the hirulog bacteria.
9. A method for using the leech growth bacteria according to any one of claims 1 to 7, wherein the leech growth bacteria is diluted and sprayed into bait to be matched with the bait, or is diluted with culture water and sprayed into a whole pond.
10. The method of using the hirulog according to claim 9, characterized in that the amount is:
feeding with bait: diluting 150 ml of the leech growth bacteria with 250 ml of well water or purified water, and directly spraying 100 kg of bait snails for feeding;
appetizing and attracting food: diluting 150 ml of the leech growth bacteria with 250 ml of well water or purified water, and directly spraying 50 kg of bait snails for feeding;
splashing of the leech growth bacterium water body: 50-100 ml of the leech growth bacteria are diluted by 50-100 times of culture water and directly sprinkled in the pond with the depth of 1 meter per mu, and the sprinkling is carried out once every 5-8 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011638866.7A CN112617027A (en) | 2020-12-31 | 2020-12-31 | Bdelloecium bdellovii and preparation method and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011638866.7A CN112617027A (en) | 2020-12-31 | 2020-12-31 | Bdelloecium bdellovii and preparation method and use method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112617027A true CN112617027A (en) | 2021-04-09 |
Family
ID=75290064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011638866.7A Pending CN112617027A (en) | 2020-12-31 | 2020-12-31 | Bdelloecium bdellovii and preparation method and use method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112617027A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078003A (en) * | 2006-05-23 | 2007-11-28 | 上海创博生态工程有限公司 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
| CN101397541A (en) * | 2007-09-29 | 2009-04-01 | 上海泓宝绿色水产科技发展有限公司 | Micro-ecological formulation promoting growth fungus containing natural growth-promoting factor and preparation method thereof |
| CN101396065A (en) * | 2007-09-29 | 2009-04-01 | 上海泓宝绿色水产科技发展有限公司 | Microecology preparation using bovine placenta protein as natural nutrient factor and preparation method thereof |
| CN105639204A (en) * | 2016-01-18 | 2016-06-08 | 山东宝来利来生物工程股份有限公司 | Biological color enhancing agent and application thereof to aquatic animal breeding |
| CN107361251A (en) * | 2017-08-24 | 2017-11-21 | 无为县慧富水蛭养殖专业合作社 | A kind of leech raising resistance feed and preparation method thereof |
| CN110742862A (en) * | 2019-11-14 | 2020-02-04 | 厦门昶科生物工程有限公司 | Astaxanthin emulsion and preparation method thereof |
| CN111000091A (en) * | 2019-12-19 | 2020-04-14 | 集美大学 | Emulsified astaxanthin compound preparation for promoting normal growth of catarrh eel |
-
2020
- 2020-12-31 CN CN202011638866.7A patent/CN112617027A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078003A (en) * | 2006-05-23 | 2007-11-28 | 上海创博生态工程有限公司 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
| CN101397541A (en) * | 2007-09-29 | 2009-04-01 | 上海泓宝绿色水产科技发展有限公司 | Micro-ecological formulation promoting growth fungus containing natural growth-promoting factor and preparation method thereof |
| CN101396065A (en) * | 2007-09-29 | 2009-04-01 | 上海泓宝绿色水产科技发展有限公司 | Microecology preparation using bovine placenta protein as natural nutrient factor and preparation method thereof |
| CN105639204A (en) * | 2016-01-18 | 2016-06-08 | 山东宝来利来生物工程股份有限公司 | Biological color enhancing agent and application thereof to aquatic animal breeding |
| CN107361251A (en) * | 2017-08-24 | 2017-11-21 | 无为县慧富水蛭养殖专业合作社 | A kind of leech raising resistance feed and preparation method thereof |
| CN110742862A (en) * | 2019-11-14 | 2020-02-04 | 厦门昶科生物工程有限公司 | Astaxanthin emulsion and preparation method thereof |
| CN111000091A (en) * | 2019-12-19 | 2020-04-14 | 集美大学 | Emulsified astaxanthin compound preparation for promoting normal growth of catarrh eel |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108707563B (en) | Composite fermentation bacterial agent and preparation method of fish protein amino acid water-soluble fertilizer | |
| CN101341927B (en) | Microbial feed additive liquid agent for pig and preparation technique | |
| CN107032504B (en) | Water quality improver for aquatic products and preparation method thereof | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN104232497A (en) | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation | |
| CN109468249A (en) | A kind of microbial bacterial agent and its application on livestock and poultry cultivation | |
| CN101591631A (en) | EM original solution and production method thereof | |
| CN111187731A (en) | Biological bottom-improving algae-culturing water quality improving microbial inoculum and preparation method thereof | |
| CN105755088A (en) | Method for inducing haematococcus pluvialis to produce C40H52O4 | |
| CN104621357A (en) | Novel liquid feed additive and preparation method thereof | |
| CN109548962A (en) | A kind of complex micro organism fungicide comprising lactobacillus plantarum and its application | |
| CN101805698A (en) | Preparation method of microbial compound preparation based on various probiotic fermenting cultures | |
| CN103667140A (en) | Compound synergism method of enterococcus faecalis and application thereof | |
| CN102334596A (en) | Preparation method of rapeseed meal fermented feed | |
| CN101748065B (en) | Production method for feeding monascus microbial preparations | |
| CN109055264A (en) | A kind of microbial bacterial agent and preparation method thereof for holothruian cultures | |
| CN109221606A (en) | A kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof | |
| CN111109176B (en) | Three-stage water purification system for aquaculture | |
| CN105272452A (en) | Fertilizer water agent for aquaculture pool and preparation method thereof | |
| CN101082034A (en) | Preparation of phycomycetes bacterium and method for restoring aquaculture environment | |
| CN112617027A (en) | Bdelloecium bdellovii and preparation method and use method thereof | |
| CN107828704A (en) | A kind of composite bacteria agent for digesting antibiotic and its application | |
| KR101750288B1 (en) | Composition for Controlling Citrus Mite and Method for Controlling Citrus Mite Using the Same | |
| TW202133731A (en) | Use of nutritional composition being prepared by adding a yeast into a soybean protein culture medium and an antrodia camphorata extract to perform fermentation | |
| CN112544782A (en) | Composite microecological preparation and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |