CN113461179A - Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment - Google Patents
Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment Download PDFInfo
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Abstract
The invention discloses rhodopseudomonas palustris ZT-MG2 and application thereof, which is preserved in China general microbiological culture Collection center in 30 months 6 in 2021, the preservation number of the strain is CGMCC NO.22804, and the classification and the name are as follows: rhodopseudomonas palustris (Rhodopseudomonas palustris). The bacterial strain is inoculated into the microcystis aeruginosa to obviously inhibit the growth of the algae, the inhibition effect is gradually obvious along with the increase of the inoculation amount, the bacterial strain has obvious inhibition and lethal effects on the microcystis aeruginosa, and the bacterial strain has obvious removal effect on algal toxins generated by the microcystis aeruginosa.
Description
Technical Field
The invention belongs to the field of environmental microorganisms, and particularly relates to rhodopseudomonas palustris with a function of specifically inhibiting algae growth and application of the rhodopseudomonas palustris in a eutrophicated water body.
Background
In recent years, with the rapid development of industrial and agricultural production and social and economic aspects in China, the amount of wastewater and domestic sewage discharged into rivers and lakes is increased, so that the environment is continuously worsened, particularly the water environment, and a large area of water bloom appears in rivers and lakes. The hazard of the water bloom is huge, on one hand, the algae is propagated in a large quantity, the dissolved oxygen content and the transparency of the water body are reduced, a large amount of aquatic organisms die, and the balance of an aquatic ecosystem is damaged. On the other hand, algal toxins secreted by various algae are accumulated in protozoa, shellfish, shrimps, fish and the like through food chains, and seriously threaten the life safety of aquaculture industry and human beings. Therefore, in the face of severe water bloom pollution, measures for improving the water environment are urgently needed.
At present, the water bloom treatment method is mainly divided into physical, chemical and biological 3 types. The physical methods include mechanical salvage, air flotation technology, ultrasonic method, adsorption method, filtration method and the like.
The method can be used as an emergency treatment measure when the blue algae explodes in a large area without obvious negative effects. The air floatation technology is a water purification method in which a large amount of highly dispersed fine bubbles are introduced or generated into water to be treated, and the fine bubbles are adhered to impurities and flocs to form floating slag with specific gravity smaller than that of the water, and the floating slag floats on the water surface by means of buoyancy to complete solid-liquid separation. The adsorbents such as the activated carbon and the like not only can remove algae at a high rate in water, but also have a certain adsorption effect on algal toxins, but also have high post-treatment cost and limit the wide-range use.
The physical algae removal methods have the advantages of simple method, no secondary pollution, capability of preventing external domain biological invasion and the like, are suitable for emergency treatment measures, but are temporary and permanent for a plurality of water bodies, need special instruments and equipment, and consume a large amount of manpower and material resources for treating large-area bloom problems.
The chemical method mainly refers to the effect of removing dryness and inhibiting algae by using a chemical algicide and a flocculant. However, chemical methods of inhibiting algae inhibit the growth of algae cells and ultimately cause the death of algae cells. But will have an influence on the growth and development of other animals and plants and will produce secondary pollution. Destroying the near-shore ecosystem and controlling the algal bloom only can play a short-term effect.
It is seen that physical and chemical control methods work in many specific cycles, but are more cost effective, efficient and more environmentally damaging than biological control methods. Under the current large background with higher and higher environmental protection requirements. The inhibition of water bloom by biological methods becomes a hotspot of research. Wherein, the microbial treatment is found to be a hot spot in the next research. The bacteria in the microorganism act on the algae in 2 ways of directly dissolving the algae and indirectly dissolving the algae. Many of the algicidal bacteria are gram-negative bacteria, and mainly include myxobacteria (Mysobacter), Cytophaga (Cytophaga), Bacillus (Bacillus), and the like. Some species of myxobacteria can directly contact algae cells by secreting cellulolytic enzymes to digest the host cell walls and thereby lyse the entire algae cell. The indirect algae dissolving aims at inhibiting algae by secreting extracellular substances such as protein, polypeptide and amino acid or by competing on nutrient substances such as nitrogen, phosphorus and the like.
Rhodopseudomonas palustris is one of photosynthetic bacteria (PSB for short), and is one of the oldest microorganisms on earth. The PSB thalli is rich in nutrition, has the protein content of 65 percent, is rich in various bioactive substances, has strong adaptability, can tolerate high-concentration organic wastewater and strong decomposition and transformation capacity, and has certain tolerance and decomposition capacity on toxic substances such as phenol, cyanogen and the like. And the rhodopseudomonas palustris is facultative anaerobe, has a fast growth period, simple culture conditions and strong adaptability in a non-pigment synthesis stage, and is suitable for the related report that the rhodopseudomonas palustris is not found to be used for inhibiting the growth of algae in wide practical application at present.
Disclosure of Invention
The invention aims to provide a novel photosynthetic bacterium belonging to the genus Rhodopseudomonas of the family Rhodospirillaceae, so as to solve the problem of large-area propagation of algae in the existing eutrophic water body.
The purpose of the invention can be realized by the following technical scheme:
the rhodopseudomonas palustris strain ZT-MG2 provided by the invention is obtained by separating a manioca at a crab pond opening of a Zhang hong Yi aquaculture center in Zhang Gugang in 11 months in 2017. The nature of which is photosynthetic bacteria that inhibit the growth of algae. The strain is preserved in China general microbiological culture Collection center (CGMCC) at 30 months 6 in 2021, with the preservation number of CGMCC: 22804, categorically named: rhodopseudomonas palustris (Rhodopseudomonas palustris) with the address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101.
the rhodopseudomonas palustris provided by the invention has the function of inhibiting the growth of algae in eutrophic water.
The rhodopseudomonas palustris provided by the invention can grow white single colonies on a TY culture medium after 24 hours under a non-illumination anaerobic condition, and can grow red single colonies on the TY culture medium after 4 days under an illumination anaerobic condition. Can grow at 18-40 deg.c and has wide adaptability and may be used in natural water in river and lake.
The invention also provides application of the rhodopseudomonas palustris ZT-MG2 in environmental sewage treatment. In some embodiments, in eutrophic water bodies, and in more particular examples, in the inhibition of algae growth in eutrophic wastewater. In a specific example, the algae is microcystis aeruginosa or/and oscillatoria tremoris.
The invention also provides a microbial inoculum containing the rhodopseudomonas palustris ZT-MG 2.
The invention also provides application of the microbial inoculum in environmental sewage treatment. In some embodiments, in eutrophic water bodies, and in more particular examples, in the inhibition of algae growth in eutrophic wastewater. In a specific example, the algae is microcystis aeruginosa or/and oscillatoria tremoris.
The invention also provides a purifying agent, which contains the rhodopseudomonas palustris ZT-MG 2.
The purifying agent of the present invention has total viable bacteria concentration of 1 × 106～1×1010CFU/ml, preferably 1X 107～1×109CFU/ml, more preferably 1X 108CFU/ml。
The invention also provides application of the purifying agent in environmental sewage treatment. In some embodiments, in eutrophic water bodies, and in more particular examples, in the inhibition of algae growth in eutrophic wastewater. In a specific example, the algae is blue algae, and preferably, the algae is microcystis aeruginosa or/and oscillatoria alga.
The invention has the beneficial effects that:
1. the research shows that the dominant rhodopseudomonas palustris which is separated and screened from different sources and can effectively inhibit the growth of algae can grow to the maximum concentration on a TY culture medium after 24 hours under the non-illumination anaerobic condition, and can normally grow at the temperature of 18-40 ℃, so that the rhodopseudomonas palustris provided by the invention has high activity and strong adaptability, and can be widely used in the treatment process of various eutrophic water bodies for inhibiting the algae.
2. The strain obtained by screening is domesticated into a high-efficiency and stable strain, the phenomenon of obviously inhibiting the growth of algae is caused when the strain is inoculated into the microcystis aeruginosa, and the inhibition effect is gradually obvious along with the increase of the inoculation amount. Further indicates that the strain has high activity, strong adaptability, strong algae inhibition capability and high removal efficiency of microcystis aeruginosa. .
3. Has obvious inhibiting and killing effect on microcystis aeruginosa and obvious removing effect on algal toxins produced by the microcystis aeruginosa, namely, the concentration of the algae can be controlled from the source and the amount of chlorophyll a and the amount of the algal toxins can be obviously reduced.
Drawings
FIG. 1 is a colony morphology of an algae inhibiting strain ZT-MG2 provided by the invention under non-light and non-anaerobic conditions;
FIG. 2 shows the colony morphology of the algal inhibiting strain ZT-MG2 provided by the present invention under the light anaerobic condition;
FIG. 3 shows the growth curve of the algal inhibiting strain ZT-MG2 under the non-light and non-anaerobic condition;
FIG. 4 is the optimum pH value of the alga-inhibiting strain ZT-MG2 provided by the invention under the non-light and non-anaerobic condition;
FIG. 5 is the optimum temperature of the alga-inhibiting strain ZT-MG2 under the non-light anaerobic condition;
FIG. 6 shows the reduction rate of the strain ZT-MG2 for Microcystis aeruginosa FACHB-930 chlorophyll a under actual simulation conditions;
FIG. 7 shows the reduction of microcystis aeruginosa FACHB-930 algal toxin by the strain ZT-MG 2;
FIG. 8 is a graph showing the inhibition of Microcystis aeruginosa FACHB-930 by the strain ZT-MG2 under actual simulation conditions;
FIG. 9 shows the lethality of the strain ZT-MG2 to Microcystis aeruginosa FACHB-930 under actual simulation conditions;
FIG. 10 is a practical diagram of the death of Microcystis aeruginosa FACHB-930 by the strain ZT-MG2 under practical simulated conditions.
Detailed Description
The present invention is further illustrated by the following examples, in which experimental procedures not specifically identified are generally performed according to methods known in the art or according to manufacturer's recommendations.
The research adopts the reduction of the strain on the algae chlorophyll a and the algae toxin as evaluation criteria.
TY medium ratios used in the following examples: 5g of peptone, 3g of yeast powder and calcium chloride (CaCl)2)0.67g, 1000mL of deionized water and 12g of agar powder are added, and the pH value is natural. 121 ℃ and 20 min.
Example 1 screening and identification of photosynthetic bacteria
1. Screening of photosynthetic bacteria
1) 5g of sludge at the bottom of a crab pond, a lobster pond and a turtle pond of a Zhang hong Yi aquaculture center are respectively added into a transparent plastic bottle filled with 500mL of photosynthetic bacteria culture medium, and the transparent plastic bottle is placed under the illumination intensity of 2000lux at the temperature of 30 ℃ for irradiation for one week.
2) 10mL of each of culture water of the mouth of the crab, the mouth of the lobster and the mouth of the turtle pond of Zhang hong Ganghou aquaculture center are added into a transparent plastic bottle filled with 500mL of photosynthetic bacteria culture medium, and the transparent plastic bottle is placed under the condition that the illumination intensity is 2000lux at the temperature of 30 ℃ for irradiation for one week.
3) When the medium in the plastic bottle became reddish brown, 1% inoculation was performed, and this was repeated 4 times until the color of the medium became red.
4) 900 mul of sterile water is pre-packaged in a 2mL centrifuge tube, and 100 mul of enriched sludge liquid and pond water are respectively packaged in the 2mL centrifuge tube containing the sterile water.
5) And finally coating the mixture on a TY solid culture medium, and placing the mixture in an anaerobic tank for culture, wherein the anaerobic tank is placed at the temperature of 30 ℃ and the illumination intensity is 2000 lux.
6) The same purification method as that of yeast.
7) And selecting a single colony, putting the single colony into a 3mL ground anaerobic bottle, and filling the anaerobic bottle with a sterilized TY culture medium. Then placing the bottle in a greenhouse with the illumination intensity of 2000lux at the temperature of 30 ℃ for irradiation for a week, placing the bottle in the greenhouse with weaker illumination for 1 day after the liquid in the anaerobic bottle turns red, and finally storing the bottle in the dark.
2. Identification of photosynthetic bacteria
2.1 extraction of Total DNA from bacteria
1) MG2 single colony was picked up and inoculated into 3ml photosynthetic bacteria liquid medium, and shake-cultured for 12 hours at 28 ℃ in a constant temperature shaker at 180 rpm/min.
2) 2mL of each of the bacterial solutions was centrifuged at 12,000rpm/min for 2min, and the supernatant was discarded.
3) The cells were resuspended in 1mL of sterile water, centrifuged at 12,000rpm/min for 2min, the supernatant was discarded, and the cells were washed 3 times.
4) Add 270. mu.L of 1 XTE buffer, blow and mix well to make the thalli fully re-suspended, add 15. mu.L of lysozyme, ice-bath for 30 min.
5) Adding 15 μ L10% SDS and 10 μ L100 μ g/mL proteinase K in sequence, mixing well with a scraper, and placing in 37 deg.C water bath for 30 min.
6) 200 μ L of 5mol/L NaCl pre-cooled at 4 ℃ is added, and the mixture is stirred vigorously by a scraper plate and mixed evenly.
7) Adding 500 μ L phenol chloroform for extraction, stirring with scraper, and centrifuging at 12,000rpm/min for 10 min.
8) After centrifugation, the mixture was divided into three layers, the supernatant from the uppermost layer was transferred to a new 2mL centrifuge tube, and extraction was repeated until no white flocculent precipitate was present at the interface.
9) The obtained supernatant was transferred to a 1.5mL EP tube, and 1 XTE buffer (about 400. mu.L) in 0.8 volume was added, and isopropyl alcohol (about 500. mu.L) in 1 volume was added thereto, and the mixture was inverted and mixed, and then placed in an ultra-low temperature refrigerator at-70 ℃ for 10 to 15 min.
10) The sample was removed, centrifuged at 12,000rpm/min for 10min, the supernatant discarded, and the pellet washed 3 times with 70% ethanol which had been pre-cooled at 4 ℃.
11) Naturally blow-drying, adding 30 μ L sterilized ddH2After O dissolved, it was stored at 4 ℃ until use.
2.216 s rRNA Gene amplification
PCR amplification was performed using 16s rRNA universal primers, the reaction system is shown in Table 2, and the primer sequences are shown in Table 1:
table 116 s rRNA Universal primer sequences
TABLE 2 PCR reaction system (volume of system 50. mu.L)
And (3) PCR reaction conditions: pre-denaturation at 95 ℃ for 5 min; denaturation at 94 ℃ for 30s, annealing at 55 ℃ for 30s, and extension at 72 ℃ for 1.5min (30 cycles); terminal extension at 72 ℃ for 10 min. And taking 3 mu L of PCR product with about 1,500bp of PCR product to carry out agarose gel electrophoresis detection, and sequencing the PCR product if the band is bright and clear, wherein the sequence is shown as SEQ ID NO. 1. Sequencing was performed by Nanjing Spojin and the resulting sequences were submitted to NCBI nucleic acid database (http:// blast. NCBI. nlm. nih. gov) for sequence alignment.
Through identification, the single bacterium ZT-MG2 is a rhodopseudomonas palustris, the bacterial strain presents a typical rhodopseudomonas palustris colony morphology, the colony morphology is round, the surface is smooth, the edge is neat, and the color is brownish red. The colony is circular under the anaerobic culture of illumination, gradually changes from white to light pink, and then changes to brownish red as shown in figure 2; under non-light anaerobic culture conditions, colonies appear as white circles with growth cycles faster than those under light anaerobic conditions, as shown in FIG. 1. In 11 months in 2017, the separated Remandong swallow is separated from a crab pond opening of an aquaculture center with high stand at Zhang Jia gang. The nature of which is photosynthetic bacteria that inhibit the growth of algae. The strain is preserved in China general microbiological culture Collection center (CGMCC) at 30 months 6 in 2021, with the preservation number of CGMCC: 22804, categorically named: rhodopseudomonas palustris (Rhodopseudomonas palustris) with the address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101.
example 2 growth curves of photosynthetic bacteria
Selecting a single colony of the strain ZT-MG2 shown in figure 1 to be arranged in TY liquid culture medium, and shake-culturing for 24h in a non-illumination constant temperature shaking table at 28 ℃ and 180rpm/min to prepare respective seed liquid. The seed solutions were inoculated into 5mL of TY liquid medium at 1% inoculum size, and 3 replicates per plant were used. Shaking and culturing at 28 deg.C and 180rpm/min in constant temperature shaking table, and measuring OD every 2 hr600. As shown in fig. 3, the optimum value has been reached in 24 hours.
Example 3 determination of optimum pH and optimum temperature of photosynthetic bacteria
A single colony of the strain ZT-MG2 shown in figure 1 is picked up and cultured in TY liquid culture medium with shaking at 28 ℃ for 24 hours to obtain a fresh seed solution. The strain was inoculated from seed solution into 5ml of TY liquid medium with pH values of 4.0, 5.0, 6.0, 7.0 and 8.0 at an inoculum size of 1%, and 3 replicates per strain were used. Shaking and culturing at 28 deg.C and 180rpm/min in a non-light constant temperature shaking table for 24 hr, and determining OD600. As shown in fig. 4. The optimum pH of the photosynthetic bacteria is between 5 and 8.
A single colony of the strain ZT-MG2 shown in FIG. 1 was selected and cultured in TY liquid medium at 28 ℃ for 24 hours to obtain a fresh seed solution. Taking respective seed liquid, inoculating 5mL of TY liquid according to 1% inoculation amountIn the medium, each strain was replicated 3 times. Shaking and culturing in a constant temperature shaker at 20 deg.C, 30 deg.C, 40 deg.C, 50 deg.C, and 180rpm/min for 24 hr, and determining OD600. As shown in FIG. 5, the optimum temperature of the strain ZT-MG2 was 25 ℃ to 45 ℃.
Example 4 inhibition of blue algae by photosynthetic bacteria in practical simulations
1. Determination of chlorophyll a
Firstly, 2mL of algae liquid which is uniformly mixed is taken, centrifuged for 15min at 3,000rpm, supernatant is removed, and then algae bodies are placed in a 10mL centrifuge tube and stored at-20 ℃ for more than 12 h.
② preheating 90 percent ethanol in a water bath kettle at the temperature of 80-85 ℃.
③ taking out the centrifuge tube, adding 8mL of hot ethanol immediately, and then placing the centrifuge tube in a water bath kettle at 80 ℃ for extraction for 2 min.
And fourthly, immediately wrapping the mixture with tin foil paper after the mixture is taken out, and extracting the mixture for 4 to 6 hours in dark in a dark place.
Fifthly, taking out the mixture and then centrifuging the mixture for 10min at 3,000rpm to obtain supernatant.
Volume fixed to 10mL with 90% ethanol
Seventhly, adjusting the solution to 0 by 90 percent ethanol, and measuring OD665 and OD 750.
⑧Cha＝(27.1×V1×OD665-OD750))/V2
V1: volume of 90% ethanol;
v2: volume of extracted algal solution
2. Algal toxin content determination
1) 1mL of the well-mixed algal solution was taken every 2 days, centrifuged at 3,000rpm for 15min, and the supernatant was collected.
2) The remaining algal bodies were added to 2mL of 75% methanol, frozen at-70 ℃ for 30min, then subjected to water bath at 40 ℃ for 30min, then shaken for 30min, and then centrifuged at 6,000rpm for 15min, and this was repeated 3 times, and the supernatants were collected.
3) The kit is balanced for 20min at room temperature, and then the algae toxin is measured
4) Setting standard product holes and sample holes, wherein 50 mu L of standard products with different concentrations are added into the standard product holes respectively; adding 10 mu L of sample to be detected into a sample hole to be detected, and then adding 40 mu L of sample diluent;
5) then 100. mu.L of detection antibody labeled with horseradish peroxidase (HRP) was added to each of the standard wells and the sample wells, the reaction wells were sealed with a sealing plate film, and incubated in an incubator at 37 ℃ for 60 min.
6) Discarding the liquid, patting dry on absorbent paper, adding 350 μ L of washing solution into each well, standing for 1min, throwing off the washing solution, patting dry on absorbent paper, and washing the plate for 5 times in this way.
7) 50. mu.L of substrate A, B was added to each well and incubated at 37 ℃ for 15min in the absence of light.
8) Add stop solution 50. mu.L per well, measure OD value of each well at 450nm wavelength within 15 min.
3. The algae liquid sample source for determining the blue algae inhibition effect is culture water of Zhang Jia gang hong high stand aquaculture center and algae therein, the algae comprises microcystis aeruginosa and Oscillating algae, in the culture water environment, the content of nitrogen and phosphorus in the water body is very high due to the metabolic waste of the culture water products and redundant baits thereof, and an important reason for water body eutrophication is that a large amount of nutrient salt is input, so the experimental condition is that a bacterial strain ZT-MG2 is added into the static culture water, the content of chlorophyll a of the blue algae in the water body is determined at different time, and the removal rate is calculated.
1) And (3) carrying out enrichment culture on the microcystis aeruginosa in the culture water with abundant nutrition in Zhang hong Kong, adding 250mL of the microcystis aeruginosa cultured to dark green into a 350mm by 40mm test tube, and placing the test tube open.
2) Then the photosynthetic bacteria ZT-MG2 cultured to logarithmic phase is inoculated into the algae liquid according to the inoculation amount of 1 percent and 5 per thousand.
3) The chlorophyll-a content was determined every 2 days.
4) Determination of lethality
Because the microcystis aeruginosa has small volume and is difficult to count under a microscope, the dyeing counting method adopting PI dyeing is adopted, namely the dyeing counting is carried out
1mL of the algae liquid mixed uniformly is taken from each group, centrifuged for 15min at 3,000rpm, and the supernatant is discarded
② adding a PI staining agent diluted by 100 times, shading and staining for 10min, discarding the PI staining agent, then adding 200 μ L PBS, centrifuging for 10min at 3,000rpm, discarding the supernatant, and washing for 3 times.
③ evenly mixing the algae liquid of the microcystis aeruginosa, sucking 20 mu L of the algae liquid, placing the mixture on a glass slide, and observing the mixture on a laser confocal microscope. The algae which emits red fluorescence is dead algae, and the algae which emits no fluorescence is survival algae. The mortality was calculated from dead/total algae.
The experimental results show that:
(1) after 8 days of experimental period, 1% and 5 ‰ ZT-MG2 was inoculated, and the color change of the algae solution is shown in FIG. 8. From apparent observation, the green color faded and turned to pale red, indicating that: compared with a treatment group (left picture) without ZT-MG2, the treatment group (right picture) inoculated with 1% and 5% of ZT-MG2 has obvious phenomenon of inhibiting the growth of algae, and the inhibition effect is gradually obvious along with the increase of inoculation amount, as shown in figure 9, the bacterial strain is inoculated into eutrophic water body with the inoculation amount of 1% and the lethality rate to the algae reaches 45% on the 2 nd day, 90% on the fourth day and 97% on the eighth day.
(2) By day 8 after inoculation of the treatment group with ZT-MG2, the treatment group with 1% inoculum size had achieved a reduction in chlorophyll a of over 85%, and the treatment group with 5% inoculum size had achieved a reduction in chlorophyll a of 77%, as shown in fig. 6. When the treatment group inoculated with ZT-MG2 detects the phycotoxin on the 8 th day, the content of the phycotoxin is obviously reduced from 2.5 mu g/mL to 1.2 mu g/mL, and the reduction amount is more than 50% of that of the treatment group not inoculated with ZT-MG2, as shown in figure 7, the content of the phycotoxin is obviously reduced. The reduction amount of chlorophyll a and algal toxin is a basic index for reflecting the water quality condition in the water quality detection of algae enrichment. The experimental results show that: the bacterial strain ZT-MG2 has obvious inhibiting effect on Microcystis aeruginosa, and has obvious removing effect on algal toxin produced by Microcystis aeruginosa.
(3) Because the microcystis aeruginosa is small in size, the microcystis aeruginosa is difficult to count by a microscope, and the microcystis aeruginosa is observed and counted by a PI dyeing mode. Since PI is a DNA-binding dye, it produces red fluorescence, but is not membrane-permeable, cannot permeate living cells, and can only stain dead cells with ruptured cell membranes. When observed under a fluorescence microscope, normal cells can not be stained, early apoptotic cells show weak red light, late apoptotic cells show enhanced red light, and dead cells show strong red fluorescence. As shown in FIG. 10, the death number of Microcystis aeruginosa in CK group is almost zero, which is consistent with the chlorophyll a condition, and the fluctuation hardly occurs. 1% of the inoculated photosynthetic bacteria can cause the death number of the microcystis aeruginosa to be obviously increased, and the death number of the microcystis aeruginosa is obviously increased. The ZT-MG2 can cause the death of microcystis aeruginosa, thereby inhibiting the growth of the microcystis aeruginosa.
Sequence listing
<110> Nanjing university of agriculture
<120> application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1357
<212> DNA
<213> Rhodopseudomonas palustris (Rhodopseudomonas palustris)
<400> 1
cggctgcccc cattgctggt tagcgcaccg tcttgcaggt aaagccaact cccatggtgt 60
gacgggcggt gtgtacaagg cccgggaacg tattcaccgt ggcatgctga tccacgatta 120
ctagcgattc caacttcatg ggctcgagtt gcagagccca atccgaactg agacggcttt 180
ttgagatttg cgaagggtca ccccttagct tcccattgtc accgccattg tagcacgtgt 240
gtagcccagc ccgtaagggc catgaggact tgacgtcatc cccaccttcc tcgcggctta 300
tcaccggcag tctccttaga gtgctcaact aaatggtagc aactaaggac gggggttgcg 360
ctcgttgcgg gacttaaccc aacatctcac gacacgagct gacgacagcc atgcagcacc 420
tgtgctccag gctccgaaga gaaggtcacg tctctgcgac cggtcctgga catgtcaagg 480
gctggtaagg ttctgcgcgt tgcgtcgaat taaaccacat gctccaccgc ttgtgcgggc 540
ccccgtcaat tcctttgagt tttaatcttg cgaccgtact ccccaggcgg aatgcttaaa 600
gcgttagctg cgccactagt gagtaaaccc actaacggct ggcattcatc gtttacggcg 660
tggactacca gggtatctaa tcctgtttgc tccccacgct ttcgtgcctc agcgtcagta 720
atggcccagt gagccgcctt cgccactggt gttcttgcga atatctacga atttcacctc 780
tacactcgca gttccactca cctctgccat actcaagact tccagtatca aaggcagttc 840
tggagttgag ctccaggctt tcacctctga cttagaaacc cgcctacgca ccctttacgc 900
ccagtgattc cgagcaacgc tagccccctt cgtattaccg cggctgctgg cacgaagtta 960
gccggggctt attcttgcgg taccgtcatt atcttcccgc acaaaagagc tttacaaccc 1020
tagggccttc atcactcacg cggcatggct ggatcaggct ttcgcccatt gtccaatatt 1080
ccccactgct gcctcccgta ggagtttgga ccgtgtctca gtcccaacgc gggcgatcat 1140
cctctcagac cagctactga tcgtcgcctt ggtgagccat tacctcacca actagctaat 1200
cagacgcggg ccgctctttc ggcgataaat ctttccccgt aagggcttat ccgctaatag 1260
cacaagtttc cctgtgttgt tccgaaccaa aaggtacgtt cccacgcgtt actcacccgt 1320
ctgccactga cgtattgcta cgcccgttcg actcgca 1357
Claims (10)
1. Rhodopseudomonas palustris ZT-MG2, is classified and named as: rhodopseudomonas palustris (Rhodopseudomonas palustris) with a preservation number of CGMCC NO. 22804.
2. An inoculant comprising the Rhodopseudomonas palustris strain ZT-MG2 of claim 1.
3. A decontaminant comprising rhodopseudomonas palustris strain ZT-MG2 according to claim 1.
4. The purifying agent as claimed in claim 3, wherein the purifying agent contains the total concentration of viable bacteriaIs 1 × 106～1×1010CFU/ml。
5. The purifying agent as claimed in claim 4, wherein the total concentration of viable bacteria in the purifying agent is 1 x 107～1×109CFU/ml。
6. The purifying agent as claimed in claim 5, wherein the total concentration of viable bacteria in the purifying agent is 1 x 108CFU/ml。
7. The bacterial strain ZT-MG2 according to claim 1, the microbial inoculum according to claim 2, or the purifying agent according to any one of claims 3 to 6, for use in environmental sewage treatment.
8. Use according to claim 7, wherein the environmental effluent is a highly eutrophicated water body.
9. The strain ZT-MG2 according to claim 1, the microbial inoculum according to claim 2 and the purifying agent according to any one of claims 3 to 6 are used for inhibiting the growth of algae in eutrophic sewage.
10. The use according to claim 9, wherein the algae is cyanobacteria, preferably, the algae is microcystis aeruginosa or/and oscillatoria.
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