CN100398657C - Method of fast degradating pine dust rosin kind substance - Google Patents
Method of fast degradating pine dust rosin kind substance Download PDFInfo
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- CN100398657C CN100398657C CNB2003101121876A CN200310112187A CN100398657C CN 100398657 C CN100398657 C CN 100398657C CN B2003101121876 A CNB2003101121876 A CN B2003101121876A CN 200310112187 A CN200310112187 A CN 200310112187A CN 100398657 C CN100398657 C CN 100398657C
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- rosin
- pine
- pine sawdust
- pine oil
- substances
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Abstract
The present invention relates to a biological fermentation technique, particularly to a method for degrading pine oil class substances in pine dust. Pine contains pine oil, the main component of turpentine in the pine oil is alpha-pinene and beta-pinene, and the stable oil substances with low molecular weight can block and inhibit the growth and development of mycelia of edible fungi; thus, the pine oil class substances are oxidized and degraded within a short time; the original nutrition of materials is not affected, the fungus and mushrooms can develop normally, and thus, pine dust is used for cultivating hericium erinaceus; a quantity of unsaturated fatty acid with volatility and low molecular weight is easily decomposed and oxidized by microbes at high temperature and high moisture; the research also needs further implementation and improvement. The present invention aims to separate and cultivate microbe groups which can degrade pine oil class substances rapidly, a small quantity of inorganic salt and organic substances are added, and a pine oil degradant additive is produced; thus, the present invention provides a method for degrading the pine oil class substances rapidly.
Description
Technical field
The invention belongs to biofermentation technique, relate generally to the method for rosin class material in the degraded pine sawdust.
Background technology
Pine tree contains rosin, turps main component in the rosin is α-Pai Xi and beta-pinene, these undersaturated lipid acid have volatility, but they are very stable at normal temperatures, and these stable lower molecular weight oily substances can hinder and suppress growing of edible fungus mycelium, therefore, at short notice with the oxidative degradation of rosin class material, neither influence the original nutrition of material, make the macro fungi normal development again, utilize the key of pine sawdust culturing edible fungus exactly.Microorganism had research for a long time to the oxidative degradation of lipid material, especially under hot and humid condition, some have volatile lower molecular weight unsaturated fatty acids and are easier to by the microbiological degradation oxidation, oxidation under the natural condition and degraded institute palpus overlong time, can't realize industrialization, research is fit to the method that rosin in the quick degraded pine sawdust of edible mushrooms is cultivated in industrialization, is still waiting further to realize and improve.
The objective of the invention is the microorganism species that separation and Culture goes out the rosin class material of can degrading fast, Fu a spot of inorganic salt and organism are made the rosin degradant additive again, and a kind of method of quick degraded rosin class material is provided.
Summary of the invention
With the rosin class material in the method degraded pine sawdust of biological fermentation, separation and Culture goes out the microorganism species of the rosin class material of can degrading fast, make the rosin degradant additive, thereby the rosin degradation process is quickened, method of the present invention is finished as follows:
1, the preparation of rosin degradant additive:
Select about 2 months pine sawdust of nature stack retting directly as the inoculum of the microbe groups of degradable rosin class material, to be linked in the fresh pine sawdust poach in 1: 2 dry weight ratio in heaps with fresh pine sawdust, nature stack retting 60-75 days, from stockpile, separated each quasi-microorganism of 50-60 strain, substratum with rosin class material filters out the very fast denseer bacterial strain 15-20 strain of growth, add the inorganic nutrient salt (sal epsom that helps microorganism growth after drying in the shade together with substratum therein, potassium primary phosphate and lime carbonate) and organic nitrogen source (urea, vitamins B etc.), add-on is the 0.1-1% of dry and soft wood chip weight, mix thoroughly, promptly make the rosin degradant additive of the rosin class material that is used for degrading fast;
2, degrade the fast method of pine sawdust rosin:
Dry and soft wood genus is screened with vibratory screening apparatus, remove foreign material and macrobead, add prepared rosin degradant additive in above-mentioned 1, add-on is the 5-15% of pine sawdust dry weight, naturally the biological fermentation that carries out in heaps, through 4-6 days, when the windrow core temperature reached 50-52 ℃, pine sawdust rosin substances content was reduced to 5.4g/kg by 25.7g/kg.Compare as follows with contained rosin substances content in 6 months pine sawdusts of pine sawdust nature windrow:
Fermentation time (my god) rosin class substances content (g/kg)
0 25.7
2 23.1
5 5.4
Nature windrow 6 months 4.8
The method of quick degraded pine sawdust rosin class material of the present invention, compare with traditional natural windrow method, pine sawdust is after adding the rosin degradant additive, 5 days rosin classes of biological fermentation material is reduced to 5.4g/kg from 25.7g/kg, and natural windrow 6 months, the content of rosin class material is just reduced to 4.8g/kg, therefore, method of the present invention is effective to quick degraded rosin class material, and this method is consumes energy neither, again not consumable material nutrition, more need not increase equipment and place, and employed rosin degradant additive low price, therefore, this method has the excellent popularization prospect.
Embodiment 1
Preparation rosin degradant additive:
Select 2 months pine sawdust of nature stack retting directly as the inoculum of the microbe groups of degradable rosin class material, to be linked in the fresh pine sawdust poach in 1: 2 dry weight ratio in heaps with fresh pine sawdust, nature stack retting 60 days, add sal epsom, potassium primary phosphate and the lime carbonate inorganic nutrient salt that helps microorganism growth after drying in the shade therein, and urea and vitamins B organic nitrogen source, add-on is 0.2% of a dry and soft wood chip weight, mix thoroughly, promptly make the rosin degradant additive of the rosin class material that is used for degrading fast.
Embodiment 2
Quick degraded pine sawdust rosin:
Dry and soft wood chip is screened with vibratory screening apparatus, remove foreign material and macrobead, add made rosin degradant additive among the embodiment 1, add-on is 5% of a dry and soft wood chip weight, naturally the biological fermentation that carries out in heaps, through 5 days, when the windrow core temperature reached 50-52 ℃, pine sawdust rosin substances content can be reduced to 5.4g/kg by 25.7g/kg.
Claims (1)
1. quick method of degraded pine sawdust rosin class material is characterized in that:
(1) select 2 months pine sawdust of nature stack retting directly as the inoculum of the microbe groups of degradable rosin class material, to be linked in the fresh pine sawdust poach in 1: 2 dry weight ratio in heaps with fresh pine sawdust, nature stack retting 60 days, add sal epsom, potassium primary phosphate and lime carbonate and urea and the vitamins B that helps microorganism growth after drying in the shade therein, add-on is 0.2% of a dry and soft wood chip weight, mix thoroughly, promptly make the rosin degradant additive of the rosin class material that is used for degrading fast;
(2) dry and soft wood chip is screened with vibratory screening apparatus, remove foreign material and macrobead, add above-mentioned rosin degradant additive, add-on is 5% of a dry and soft wood chip weight, naturally the biological fermentation that carries out in heaps, through 5 days, when the windrow core temperature reached 50-52 ℃, pine sawdust rosin substances content was reduced to 5.4g/kg by 25.7g/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101121876A CN100398657C (en) | 2003-11-19 | 2003-11-19 | Method of fast degradating pine dust rosin kind substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101121876A CN100398657C (en) | 2003-11-19 | 2003-11-19 | Method of fast degradating pine dust rosin kind substance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1618979A CN1618979A (en) | 2005-05-25 |
| CN100398657C true CN100398657C (en) | 2008-07-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101121876A Expired - Fee Related CN100398657C (en) | 2003-11-19 | 2003-11-19 | Method of fast degradating pine dust rosin kind substance |
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| CN (1) | CN100398657C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110266831A1 (en) * | 2010-06-09 | 2011-11-03 | Raymond Edward Kalisz | Cut sheet stock mycelium and method |
| CN102440150B (en) * | 2011-10-14 | 2013-04-17 | 姚安农哈哈食用菌开发有限公司 | Processing method for planting edible fungus by replacing hardwood forest wood chips with pine wood chips |
| CN105265163A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Method for degrading harmful substances hindering edible fungi growth in pine/cedar/eucalyptus wood chips |
| CN105265164A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Method for cultivating edible mushrooms by pine/cedar/eucalyptus wood chips |
| CN105503292A (en) * | 2016-01-21 | 2016-04-20 | 北京健源泽农科技有限公司 | Preparation method of organic plant cultivation substrate |
| CN107684821B (en) * | 2017-11-17 | 2020-05-22 | 北京沃太斯环保科技发展有限公司 | Biofiltration filler for VOCs and malodorous gas treatment and preparation method thereof |
| CN109105157A (en) * | 2018-10-25 | 2019-01-01 | 福清市火麒麟食用菌技术开发有限公司 | A kind of preparation method of silk ball bacteria fermentation culture medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85103034A (en) * | 1985-04-16 | 1986-10-15 | 广西灵川县食用菌种厂 | Xianggu mashroom open cultivate technique by bagasse mixed with liquid fungus species |
| CN1058691A (en) * | 1991-03-26 | 1992-02-19 | 福建农学院食用菌实验场 | The mushroom grass replacing wood and grain method for cultivating adible mushroom |
| CN1303837A (en) * | 1999-11-26 | 2001-07-18 | 李翼 | Method for preparing bio-active agent by fermentation of organic wastes |
-
2003
- 2003-11-19 CN CNB2003101121876A patent/CN100398657C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85103034A (en) * | 1985-04-16 | 1986-10-15 | 广西灵川县食用菌种厂 | Xianggu mashroom open cultivate technique by bagasse mixed with liquid fungus species |
| CN1058691A (en) * | 1991-03-26 | 1992-02-19 | 福建农学院食用菌实验场 | The mushroom grass replacing wood and grain method for cultivating adible mushroom |
| CN1303837A (en) * | 1999-11-26 | 2001-07-18 | 李翼 | Method for preparing bio-active agent by fermentation of organic wastes |
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| CN1618979A (en) | 2005-05-25 |
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Address after: 510070 No. 100 martyrs Middle Road, Guangdong, Guangzhou Co-patentee after: Huankai Microbes Tech Co., Ltd., Guangdong Patentee after: Guangdong Institute of Microbiology (Guangdong province microbiological analysis and testing center) Address before: 510070 No. 100 martyrs Middle Road, Guangdong, Guangzhou Co-patentee before: Huankai Microbes Tech Co., Ltd., Guangdong Patentee before: Guangdong Institute of Microbiology |
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Granted publication date: 20080702 Termination date: 20201119 |