CN111170807A - Large-scale cultivation method for forest humic bacterial manure - Google Patents
Large-scale cultivation method for forest humic bacterial manure Download PDFInfo
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- CN111170807A CN111170807A CN201911398205.9A CN201911398205A CN111170807A CN 111170807 A CN111170807 A CN 111170807A CN 201911398205 A CN201911398205 A CN 201911398205A CN 111170807 A CN111170807 A CN 111170807A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a large-scale culture method of forest humic acid bacterial manure, and belongs to the field of biological fertilizers. The method comprises the following steps: collecting humus at the bottom of fallen leaves in a forest as parent strains; fully mixing the parent strain and the nutrient material, and sprinkling water to keep the water content of the mixture at 30-40%; mixing and piling for 30-45 days at normal temperature; circularly culturing for multiple times. The invention adopts forest humus soil as parent strain, the strain is diversified, and the specific nutrient material is adopted for pile fermentation, so that the humus soil is diversified in microorganism and good in permeability. The humus soil is applied to soil for crop cultivation, so that the use amount of the fertilizer can be reduced by 80%, the yield can be increased by 1.5-1.8 times, and the humus soil has a remarkable effect on ecological planting.
Description
Technical Field
The invention relates to the field of biological fertilizers, and in particular relates to a large-scale culture method of forest humic bacterial fertilizers.
Background
The humus soil is formed by long-term rotting and fermentation of dead branches and leaves of trees in surface soil layers in forests. The fertilizer contains a large amount of microbial bacteria, organic matters and various nutrient elements of different types through microbial decomposition, and is a good fertilizer in the planting industry. The existing humus soil is generally formed by directly mixing poultry manure with soil and then piling the mixture, and although the inside of the humus soil contains a large amount of nutrient elements and organic matters, the variety of microorganisms is few. Or directly adding microbial powder into humus soil, but the strain is still single.
The forest humus soil contains a large amount of organic matters, and the humus soil contains diversified strains, so that the soil permeability can be improved, the soil aggregate structure can be increased, and the soil is fertile. Although humus soil is widely and routinely used, large-scale cultivation of diversified primary microorganisms through forest humus soil is still in the blank stage.
Disclosure of Invention
The invention provides a large-scale cultivation method of forest humic bacterial manure, which is simple in method and diversified in strain distribution.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a large-scale cultivation method of forest humic bacterial manure comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, and sprinkling water to keep the water content of the mixture at 30-40%; mixing and piling for 30-45 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, and mixing thoroughly, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, and mixing thoroughly, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
Further, the humus soil in the step (1) is humus soil with the fallen leaf bottom not more than 10 cm.
Further, in the step (1), the ratio of the parent strain to the nutrient is 1: (1-3).
Preferably, the ratio of the parent strain to the nutrient in the step (1) is 1: 2.
further, in the step (2), the ratio of the first culture soil to the supplemented nutrient materials is 1: (0.5-1).
Preferably, the ratio of the first culture soil to the supplemented nutrient materials in the step (2) is 1: 0.8.
further, in the step (3), the ratio of the second culture soil to the supplemented nutrient materials is 1: (0.5-1).
Preferably, the ratio of the second culture soil to the supplemented nutrient materials in the step (3) is 1: 0.5.
further, the nutrient material comprises the following components in parts by weight: 10-20 parts of 1-2 cm-long crushed straw or wood chips, 20-30 parts of vegetable leaves, 30-40 parts of brown sugar, 20-30 parts of bagasse and 5-10 parts of fruit residues.
Preferably, the nutrient material comprises the following components in parts by weight: 15 parts of 1-2 cm-long crushed straws or sawdust, 30 parts of vegetable leaves, 30 parts of brown sugar, 20 parts of bagasse and 5 parts of fruit residues.
Further, the pile fermentation is to spread a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
The method for culturing the forest humic acid bacterial fertilizer on a large scale has the beneficial effects that:
(1) the invention adopts forest humus soil as parent strain, the strain is diversified, and the pile fermentation is carried out by adopting specific nutrient material, the microorganism is diversified, and the humus soil has good permeability.
(2) The humus soil is rich in organic matters and nutrient elements, and the application of the humus soil to soil for crop cultivation can reduce the use amount of chemical fertilizers by 80 percent and increase the yield by 1.5 to 1.8 times, thereby having remarkable effect on ecological planting.
Detailed Description
The present invention will be described in further detail with reference to the following examples.
Example 1
A large-scale cultivation method of forest humic bacterial manure comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains; wherein the humus soil is humus soil with the bottom of fallen leaves not more than 10 cm;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, wherein the weight percentage of the parent strain to the nutrient material is 1:2, and watering to keep the water content of the mixture at 30%; mixing and piling for 30 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, and fully mixing according to the proportion of 1:0.5, and sprinkling water to keep the water content of the mixture at 35%; performing pile fermentation culture at normal temperature for 20 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, and mixing at a ratio of 1:0.5, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
The nutrient material comprises the following components in parts by weight: 10 parts of 1-2 cm-long crushed straws or sawdust, 30 parts of vegetable leaves, 40 parts of brown sugar, 20 parts of bagasse and 5 parts of fruit residues.
Piling, namely paving a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
Example 2
A large-scale cultivation method of forest humic bacterial manure comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains; wherein the humus soil is humus soil with the bottom of fallen leaves not more than 10 cm;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, wherein the weight percentage of the parent strain to the nutrient material is 1:3, and watering to keep the water content of the mixture at 30%; mixing and piling for 30 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, fully mixing according to the proportion of 1:1, and sprinkling water to keep the water content of the mixture to be 35%; performing pile fermentation culture at normal temperature for 20 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, mixing at a ratio of 1:1, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
The nutrient material comprises the following components in parts by weight: 10 parts of 1-2 cm-long crushed straws or sawdust, 30 parts of vegetable leaves, 40 parts of brown sugar, 20 parts of bagasse and 5 parts of fruit residues.
Piling, namely paving a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
Example 3
A large-scale cultivation method of forest humic bacterial manure comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains; wherein the humus soil is humus soil with the bottom of fallen leaves not more than 10 cm;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, wherein the weight percentage of the parent strain to the nutrient material is 1:2, and watering to keep the water content of the mixture at 30%; mixing and piling for 30 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, and fully mixing according to the proportion of 1:0.8, and sprinkling water to keep the water content of the mixture at 35%; performing pile fermentation culture at normal temperature for 20 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, and mixing at a ratio of 1:0.5, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
The nutrient material comprises the following components in parts by weight: 10 parts of 1-2 cm-long crushed straws or sawdust, 30 parts of vegetable leaves, 40 parts of brown sugar, 20 parts of bagasse and 5 parts of fruit residues.
Piling, namely paving a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
Example 4
A large-scale cultivation method of forest humic bacterial manure comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains; wherein the humus soil is humus soil with the bottom of fallen leaves not more than 10 cm;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, wherein the weight percentage of the parent strain to the nutrient material is 1:2, and watering to keep the water content of the mixture at 30%; mixing and piling for 30 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, and fully mixing according to the proportion of 1:0.8, and sprinkling water to keep the water content of the mixture at 35%; performing pile fermentation culture at normal temperature for 20 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, and mixing at a ratio of 1:0.5, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
The nutrient material comprises the following components in parts by weight: 20 parts of 1-2 cm-long crushed straws or sawdust, 20 parts of vegetable leaves, 30 parts of brown sugar, 30 parts of bagasse and 10 parts of fruit residues.
Piling, namely paving a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
Example 5
A large-scale cultivation method of forest humic bacterial manure comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains; wherein the humus soil is humus soil with the bottom of fallen leaves not more than 10 cm;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, wherein the weight percentage of the parent strain to the nutrient material is 1:2, and watering to keep the water content of the mixture at 30%; mixing and piling for 30 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, and fully mixing according to the proportion of 1:0.8, and sprinkling water to keep the water content of the mixture at 35%; performing pile fermentation culture at normal temperature for 20 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, and mixing at a ratio of 1:0.5, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
The nutrient material comprises the following components in parts by weight: 15 parts of 1-2 cm-long crushed straws or sawdust, 30 parts of vegetable leaves, 30 parts of brown sugar, 20 parts of bagasse and 5 parts of fruit residues.
Piling, namely paving a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and embodiments of the present invention are explained herein by using specific examples, and the descriptions of the embodiments are only used to help understanding the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the present description should not be construed as a limitation to the present invention.
Claims (7)
1. A large-scale cultivation method of forest humic bacterial manure is characterized by comprising the following steps: the method comprises the following steps:
(1) collecting humus at the bottom of fallen leaves in a forest as parent strains;
(2) a first culture stage: fully mixing the parent strain and the nutrient material, and sprinkling water to keep the water content of the mixture at 30-40%; mixing and piling for 30-45 days at normal temperature to obtain first culture soil;
(3) and (3) second culture: supplementing nutrient materials after the first culture stage, and mixing thoroughly, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain second culture soil;
(4) and (3) third culture: supplementing nutrient materials after the second culture stage, and mixing thoroughly, and sprinkling water to keep the water content of the mixture at 30-40%; performing pile fermentation culture at normal temperature for 20-30 days to obtain third culture soil;
(5) taking the third culture soil as a parent strain, and adding nutrient materials for circulating culture.
2. The method for culturing forest humic bacterial manure on a large scale according to claim 1, which is characterized by comprising the following steps: in the step (1), the humus soil is humus soil with the fallen leaf bottom not more than 10 cm.
3. The method for culturing forest humic bacterial manure on a large scale according to claim 1, which is characterized by comprising the following steps: in the step (1), the ratio of the parent strain to the nutrient material is 1: (1-3).
4. The method for culturing forest humic bacterial manure on a large scale according to claim 1, which is characterized by comprising the following steps: in the step (2), the proportion of the first culture soil to the supplemented nutrient materials is 1: (0.5-1).
5. The method for culturing forest humic bacterial manure on a large scale according to claim 1, which is characterized by comprising the following steps: in the step (3), the ratio of the second culture soil to the supplemented nutrient materials is 1: (0.5-1).
6. The method for culturing forest humic bacterial manure on a large scale according to claim 1, which is characterized by comprising the following steps: the nutrient material comprises the following components in parts by weight: 10-20 parts of 1-2 cm-long crushed straw or wood chips, 20-30 parts of vegetable leaves, 30-40 parts of brown sugar, 20-30 parts of bagasse and 5-10 parts of fruit residues.
7. The method for culturing forest humic bacterial manure on a large scale according to claim 1, which is characterized by comprising the following steps: the pile fermentation is to spread a layer of 3-5 cm straws on the surface of the mixture; turning over in the pile fermentation process to ensure that the temperature in the pile fermentation is not more than 40 ℃ and oxygen is available.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113068561A (en) * | 2021-04-06 | 2021-07-06 | 广西壮族自治区药用植物园 | Organic planting and converting method for illicium verum forest |
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| CN113068561A (en) * | 2021-04-06 | 2021-07-06 | 广西壮族自治区药用植物园 | Organic planting and converting method for illicium verum forest |
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Application publication date: 20200519 |