CL2017001225A1 - Materiales y métodos para aumentar el contenido de tocoferol en semillas oleaginosas. - Google Patents

Materiales y métodos para aumentar el contenido de tocoferol en semillas oleaginosas.

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Publication number
CL2017001225A1
CL2017001225A1 CL2017001225A CL2017001225A CL2017001225A1 CL 2017001225 A1 CL2017001225 A1 CL 2017001225A1 CL 2017001225 A CL2017001225 A CL 2017001225A CL 2017001225 A CL2017001225 A CL 2017001225A CL 2017001225 A1 CL2017001225 A1 CL 2017001225A1
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CL
Chile
Prior art keywords
polinucleotide
tocopherol
methods
content
increase
Prior art date
Application number
CL2017001225A
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English (en)
Inventor
Carl Andre
Original Assignee
Basf Plant Science Co Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54601758&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2017001225(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Basf Plant Science Co Gmbh filed Critical Basf Plant Science Co Gmbh
Publication of CL2017001225A1 publication Critical patent/CL2017001225A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/31Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D7/00Edible oil or fat compositions containing an aqueous phase, e.g. margarines
    • A23D7/02Edible oil or fat compositions containing an aqueous phase, e.g. margarines characterised by the production or working-up
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12Fatty acids or derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/185Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19Carboxylic acids, e.g. valproic acid
    • A61K31/20Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A61K31/202Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
    • CCHEMISTRY; METALLURGY
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
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    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19003Linoleoyl-CoA desaturase (1.14.19.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19006DELTA12-fatty-acid desaturase (1.14.19.6), i.e. oleoyl-CoA DELTA12 desaturase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y602/00Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003Long-chain-fatty-acid-CoA ligase (6.2.1.3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

<p>LA PRESENTE INVENCIÓN SE REFIERE POR LO GENERAL AL CAMPO DE LA BIOLOGÍA MOLECULAR Y A INQUIETUDES PARA AUMENTAR EL CONTENIDO DE TOCOFEROL DE UNA PLANTA EN COMPARACIÓN CON UNA PLANTA DE CONTROL, QUE COMPRENDE EXPRESAR EN UNA PLANTA AL MENOS UN POLINUCLEÓTIDO QUE CODIFICA UNA DELTA-12-DESATURASA, AL MENOS UN POLINUCLEÓTIDO QUE CODIFICA UNA DELTA-6-DESATURASA, AL MENOS UN POLINUCLEÓTIDO QUE CODIFICA UNA DELTA-6-ELONGASA, Y AL MENOS UN POLINUCLEÓTIDO QUE CODIFICA UNA DELTA-5-DESATURASA. LA PRESENTE INVENCIÓN TAMBIÉN SE REFIERE A MÉTODOS PARA LA PRODUCCIÓN DE ACEITE, COMPOSICIONES QUE CONTIENEN ÁCIDOS GRASOS O LÍPIDOS, Y A ACEITES Y LÍPIDOS COMO TALES.</p>
CL2017001225A 2014-11-14 2017-05-12 Materiales y métodos para aumentar el contenido de tocoferol en semillas oleaginosas. CL2017001225A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462079622P 2014-11-14 2014-11-14
US201562234373P 2015-09-29 2015-09-29

Publications (1)

Publication Number Publication Date
CL2017001225A1 true CL2017001225A1 (es) 2017-12-01

Family

ID=54601758

Family Applications (4)

Application Number Title Priority Date Filing Date
CL2017001225A CL2017001225A1 (es) 2014-11-14 2017-05-12 Materiales y métodos para aumentar el contenido de tocoferol en semillas oleaginosas.
CL2017001223A CL2017001223A1 (es) 2014-11-14 2017-05-12 Materiales y métodos para la producción de pufa, y composiciones que contienen pufa
CL2017001221A CL2017001221A1 (es) 2014-11-14 2017-05-12 Modificación de lípidos de plantas que contienen pufas
CL2018003505A CL2018003505A1 (es) 2014-11-14 2018-12-06 Materiales y métodos para la producción de pufa, y composiciones que contienen pufa.(divisional solicitud 201701223)

Family Applications After (3)

Application Number Title Priority Date Filing Date
CL2017001223A CL2017001223A1 (es) 2014-11-14 2017-05-12 Materiales y métodos para la producción de pufa, y composiciones que contienen pufa
CL2017001221A CL2017001221A1 (es) 2014-11-14 2017-05-12 Modificación de lípidos de plantas que contienen pufas
CL2018003505A CL2018003505A1 (es) 2014-11-14 2018-12-06 Materiales y métodos para la producción de pufa, y composiciones que contienen pufa.(divisional solicitud 201701223)

Country Status (14)

Country Link
US (12) US11260095B2 (es)
EP (4) EP4151733A1 (es)
JP (7) JP7067926B2 (es)
KR (2) KR102580142B1 (es)
CN (6) CN107580629B (es)
AU (11) AU2015344993C9 (es)
CA (5) CA2967128A1 (es)
CL (4) CL2017001225A1 (es)
EC (3) ECSP17036764A (es)
MX (4) MX2017006291A (es)
MY (1) MY184649A (es)
PH (4) PH12017500876A1 (es)
SG (1) SG11201703858YA (es)
WO (6) WO2016075327A2 (es)

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