CA2377665A1 - Methodes et compositions permettant d'analyser des analytes - Google Patents
Methodes et compositions permettant d'analyser des analytes Download PDFInfo
- Publication number
- CA2377665A1 CA2377665A1 CA002377665A CA2377665A CA2377665A1 CA 2377665 A1 CA2377665 A1 CA 2377665A1 CA 002377665 A CA002377665 A CA 002377665A CA 2377665 A CA2377665 A CA 2377665A CA 2377665 A1 CA2377665 A1 CA 2377665A1
- Authority
- CA
- Canada
- Prior art keywords
- mutant
- binding
- enzyme
- analyte
- sah
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Inorganic Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
L'invention concerne des compositions et des méthodes permettant d'analyser des analytes, de préférence, des analytes à petites molécules. Ces méthodes d'analyse utilisent, au lieu d'anticorps ou de molécules qui se lient aux analytes ou aux substrats cibles, des enzymes modifiées, appelées enzymes de piégeage par substrat. Ces enzymes modifiées retiennent une affinité de liaison ou présentent une affinité de liaison améliorée pour un substrat ou un analyte cible, mais elles présentent une activité catalytique atténuée par rapport au substrat ou à l'analyte. L'invention concerne également ces enzymes modifiées, et notamment, des S-adénosylhomocystéine (SAH) hydrolases mutantes, retenant sensiblement une affinité de liaison ou présentant une affinité de liaison améliorée pour l'homocystéine ou la S-adénosylhomocystéine, mais présentant une activité catalytique atténuée. L'invention concerne en outre des conjugués des enzymes modifiées et une substance auxiliaire, tel que des agents assistant à la purification ou à la liaison sur un support solide.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/347,878 | 1999-07-06 | ||
US09/347,878 US6376210B1 (en) | 1999-07-06 | 1999-07-06 | Methods and compositions for assaying analytes |
US45720599A | 1999-12-06 | 1999-12-06 | |
US09/457,205 | 1999-12-06 | ||
PCT/US2000/018057 WO2001002600A2 (fr) | 1999-07-06 | 2000-06-30 | Methodes et compositions permettant d'analyser des analytes |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2377665A1 true CA2377665A1 (fr) | 2001-01-11 |
Family
ID=26995460
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002377665A Abandoned CA2377665A1 (fr) | 1999-07-06 | 2000-06-30 | Methodes et compositions permettant d'analyser des analytes |
Country Status (4)
Country | Link |
---|---|
AU (1) | AU5781800A (fr) |
CA (1) | CA2377665A1 (fr) |
GB (1) | GB2368641B (fr) |
WO (1) | WO2001002600A2 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108486007A (zh) * | 2018-03-22 | 2018-09-04 | 嘉兴益诺康生物科技有限公司 | 一种用于降低血尿酸的干酪乳杆菌株、益生菌组合物及其应用 |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7192729B2 (en) | 1999-07-06 | 2007-03-20 | General Atomics | Methods for assaying homocysteine |
AU2003218600C1 (en) | 2002-03-26 | 2009-12-17 | Zensun (Shanghai) Science & Technology Co., Ltd. | ERBB3 based methods and compositions for treating neoplasms |
WO2003106971A2 (fr) | 2002-06-17 | 2003-12-24 | The Regents Of The University Of Colorado | Variants de la cystathionine $g(b)-synthase humaine et leurs procedes de production |
US7098189B2 (en) | 2002-12-16 | 2006-08-29 | Kimberly-Clark Worldwide, Inc. | Wound and skin care compositions |
WO2004061422A2 (fr) * | 2002-12-31 | 2004-07-22 | Rodi Pharma, Inc. | Jeux ordonnes d'enzymes de liaison et procedes proteomiques haut debit |
US7097968B2 (en) | 2003-07-10 | 2006-08-29 | General Atomics | Methods and compositions for assaying homocysteine |
US8476034B2 (en) | 2003-07-10 | 2013-07-02 | General Atomics | Methods and compositions for assaying homocysteine |
US7485666B2 (en) | 2004-06-17 | 2009-02-03 | Kimberly-Clark Worldwide, Inc. | Vaginal health products |
US9675678B2 (en) | 2013-01-29 | 2017-06-13 | The Regents Of The University Of Colorado, A Body Corporate | Compositions and methods for treatment of homocystinuria |
WO2015006934A1 (fr) * | 2013-07-17 | 2015-01-22 | 国家纳米科学中心 | Puce de criblage de médicaments à petites molécules, procédé de construction correspondant et application correspondante |
CN104111337B (zh) * | 2014-05-07 | 2016-01-06 | 山东博科生物产业有限公司 | 抗干扰性强的同型半胱氨酸检测试剂盒 |
CN106434786B (zh) * | 2015-08-21 | 2020-08-25 | 光明乳业股份有限公司 | 一种由干酪乳杆菌发酵制备胞外多糖的方法 |
LT3373922T (lt) | 2015-11-09 | 2022-04-11 | The Regents Of The University Of Colorado, A Body Corporate | Kompozicijos ir būdai skirti naudoti homocistinurijos gydyme |
WO2018195006A1 (fr) | 2017-04-17 | 2018-10-25 | The Regents Of The University Of Colorado, A Body Corporate | Optimisation d'enzymothérapie de remplacement pour le traitement de l'homocystinurie |
CN110097928B (zh) * | 2019-04-17 | 2022-03-11 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | 一种基于肠道菌群预测组织微量元素含量的预测方法和预测模型 |
CN114460159B (zh) * | 2022-02-17 | 2023-11-03 | 河南中医药大学 | 基于photo-ATRP信号放大策略的ALP活性检测试剂盒及其使用方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3881931T2 (de) * | 1987-04-10 | 1993-12-16 | Univ Southern Australia | Bestimmung von Kaliumionen in Flüssigkeiten. |
GB9022304D0 (en) * | 1990-10-15 | 1990-11-28 | Ares Serono Res & Dev Ltd | Assay technique |
ES2196116T5 (es) * | 1992-01-22 | 2012-04-11 | Axis-Shield Asa | Ensayo de homocisteína |
US5679548A (en) * | 1993-02-02 | 1997-10-21 | The Scripps Research Institute | Methods for producing polypeptide metal binding sites and compositions thereof |
WO1998020156A1 (fr) * | 1996-11-04 | 1998-05-14 | Merck Frosst Canada & Co. | Test de liaison d'hydrolases |
DE19757571A1 (de) * | 1997-12-23 | 1999-06-24 | Hartmut Prof Dr Oswald | Ein einfacher und hochempfindlicher Nachweis von Adenosin in kleinen Proben |
-
2000
- 2000-06-30 WO PCT/US2000/018057 patent/WO2001002600A2/fr active Application Filing
- 2000-06-30 GB GB0200425A patent/GB2368641B/en not_active Expired - Fee Related
- 2000-06-30 CA CA002377665A patent/CA2377665A1/fr not_active Abandoned
- 2000-06-30 AU AU57818/00A patent/AU5781800A/en not_active Abandoned
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108486007A (zh) * | 2018-03-22 | 2018-09-04 | 嘉兴益诺康生物科技有限公司 | 一种用于降低血尿酸的干酪乳杆菌株、益生菌组合物及其应用 |
Also Published As
Publication number | Publication date |
---|---|
WO2001002600A3 (fr) | 2002-01-10 |
WO2001002600A2 (fr) | 2001-01-11 |
AU5781800A (en) | 2001-01-22 |
GB0200425D0 (en) | 2002-02-27 |
GB2368641A (en) | 2002-05-08 |
WO2001002600A9 (fr) | 2002-07-25 |
GB2368641B (en) | 2004-10-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |