BRPI0407911A - método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos - Google Patents

método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos

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Publication number
BRPI0407911A
BRPI0407911A BRPI0407911-6A BRPI0407911A BRPI0407911A BR PI0407911 A BRPI0407911 A BR PI0407911A BR PI0407911 A BRPI0407911 A BR PI0407911A BR PI0407911 A BRPI0407911 A BR PI0407911A
Authority
BR
Brazil
Prior art keywords
producing
neutral metalloprotease
transglutaminase
microbial
pro
Prior art date
Application number
BRPI0407911-6A
Other languages
English (en)
Inventor
Yukiko Umezawa
Keiichi Yokoyama
Yoshimi Kikuchi
Masayo Date
Norimasa Onishi
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BRPI0407911A publication Critical patent/BRPI0407911A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODO PARA PRODUZIR UMA TRANSGLUTAMINASE ATIVA MICROBIANA A PARTIR DE UMA PRó-TRANSGLUTAMINASE MICROBIANA, METALOPROTEASE NEUTRA DE ACTINOMICETOS, MOLéCULA DE áCIDO NUCLEICO, E, MéTODO PARA PRODUZIR UMA METALOPROTEASE NEUTRA DE ACTINOMICETOS". A presente invenção fornece uma metaloprotease neutra de actinomicetos que seletivamente cliva uma parte de pró-estrutura de uma pró-transglutaminase microbiana e um gene codificando a mesma. Uma transglutaminase ativa microbiana da qual a parte de pró-estrutura foi excisada pode ser produzida cultivando-se um microorganismo tendo um gene que codifica a metaloprotease neutra de actinomicetos descrita acima introduzido no mesmo, permitindo que o microorganismo produza a metaloprotease neutra de actinomicetos e depois tratando a pró-transglutaminase microbiana com o mesmo.
BRPI0407911-6A 2003-03-07 2004-03-05 método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos BRPI0407911A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003061623 2003-03-07
PCT/JP2004/002923 WO2004078973A1 (ja) 2003-03-07 2004-03-05 微生物トランスグルタミナーゼの製造法

Publications (1)

Publication Number Publication Date
BRPI0407911A true BRPI0407911A (pt) 2006-02-14

Family

ID=32958965

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0407911-6A BRPI0407911A (pt) 2003-03-07 2004-03-05 método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos

Country Status (13)

Country Link
US (2) US7704707B2 (pt)
EP (1) EP1602722B1 (pt)
JP (1) JP4482938B2 (pt)
KR (1) KR20050106502A (pt)
CN (1) CN100381569C (pt)
AT (1) ATE496995T1 (pt)
BR (1) BRPI0407911A (pt)
CA (1) CA2518049C (pt)
DE (1) DE602004031198D1 (pt)
DK (1) DK1602722T3 (pt)
ES (1) ES2356149T3 (pt)
RU (1) RU2316594C2 (pt)
WO (1) WO2004078973A1 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SK287800B6 (sk) * 2000-01-21 2011-10-04 Ajinomoto Co., Inc. Spôsob výroby L-lyzínu
BRPI0407911A (pt) 2003-03-07 2006-02-14 Ajinomoto Kk método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos
WO2005103278A1 (ja) * 2004-04-20 2005-11-03 Ajinomoto Co., Inc. タンパク質の製造法
JP4631436B2 (ja) * 2004-12-22 2011-02-16 岡山県 メタロエンドペプチダーゼ
CN101248176B (zh) * 2005-08-30 2011-05-11 味之素株式会社 制备蛋白酶活性降低的含转谷氨酰胺酶制品的方法
JP4973496B2 (ja) * 2005-08-30 2012-07-11 味の素株式会社 トランスグルタミナーゼ含有物のプロテアーゼ活性測定方法及び接着用トランスグルタミナーゼ製剤
EP2123756B1 (en) * 2007-02-15 2014-06-04 Ajinomoto Co., Inc. Transglutaminase having disulfide bond introduced therein
PL2138049T3 (pl) 2007-03-29 2014-11-28 Ajinomoto Kk Preparat enzymatyczny do adhezji i sposób wytwarzania uformowanego adhezyjnie produktu żywnościowego
WO2009066785A1 (ja) 2007-11-19 2009-05-28 Ajinomoto Co., Inc. 繊維加工物及びその製造法
EP2255823A4 (en) * 2008-02-15 2011-03-09 Ajinomoto Kk GUT IMMUNE-STIMULATOR
EP2302066A4 (en) * 2008-07-09 2012-02-08 Ajinomoto Kk METHOD FOR PRODUCING AMINOHYDROXYBENZOIC ACID
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
EP2404999B1 (en) 2009-03-06 2015-04-29 Ajinomoto Co., Inc. Thermotolerant transglutaminase originating in actinomyces
WO2012176919A1 (ja) 2011-06-23 2012-12-27 味の素株式会社 免疫グロブリン折りたたみ構造を持つタンパク質と、サブユニット構造となりうるタンパク質とを融合させた単量体タンパク質からなる多量体タンパク質の調製方法
JP6064912B2 (ja) 2011-10-25 2017-01-25 味の素株式会社 タンパク質の分泌生産法
WO2013065869A1 (en) 2011-11-02 2013-05-10 Ajinomoto Co.,Inc. Method for secretory production of protein
IN2014CN04002A (pt) * 2011-11-02 2015-10-23 Ajinomoto Kk
CN104379742A (zh) 2012-05-29 2015-02-25 味之素株式会社 生产3-乙酰氨基-4-羟基苯甲酸的方法
CN107075492B (zh) * 2014-11-10 2021-07-27 诺维信公司 金属蛋白酶及其用途
CN108103041A (zh) * 2018-02-02 2018-06-01 泰兴市东圣生物科技有限公司 一种热稳定微生物转谷氨酰胺酶及其编码基因
CN113528479B (zh) * 2020-04-13 2023-01-24 中国科学院微生物研究所 一种转谷氨酰胺酶的高效制备方法及其专用工程菌
CN113528478B (zh) * 2020-04-13 2023-01-20 中国科学院微生物研究所 一种高效生产转谷氨酰胺酶的方法及其专用工程菌
KR102277403B1 (ko) * 2021-01-27 2021-07-14 씨제이제일제당 주식회사 신규한 리보뉴클레아제 p 변이체 및 이를 이용한 l-글루탐산 생산 방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0665280B2 (ja) * 1987-03-04 1994-08-24 味の素株式会社 タンパクゲル化剤及びそれを用いるタンパクのゲル化方法
JPH0970291A (ja) 1995-06-30 1997-03-18 Ajinomoto Co Inc 人工トランスポゾンを用いた遺伝子増幅方法
CN1174889A (zh) * 1997-06-16 1998-03-04 中国医学科学院医药生物技术研究所 一种链霉菌产生的新型纤溶酶
KR100565030B1 (ko) * 1999-09-30 2006-03-30 아지노모토 가부시키가이샤 트랜스글루타미나제의 제조방법
WO2002081694A1 (fr) * 2001-03-30 2002-10-17 Ajinomoto Co.,Inc. Procede de secretion et de production de proteine
BRPI0407911A (pt) * 2003-03-07 2006-02-14 Ajinomoto Kk método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos
WO2005103278A1 (ja) * 2004-04-20 2005-11-03 Ajinomoto Co., Inc. タンパク質の製造法

Also Published As

Publication number Publication date
DK1602722T3 (da) 2011-05-16
WO2004078973A1 (ja) 2004-09-16
CN1759180A (zh) 2006-04-12
DE602004031198D1 (de) 2011-03-10
JPWO2004078973A1 (ja) 2006-06-08
US20100159560A1 (en) 2010-06-24
US7704707B2 (en) 2010-04-27
ES2356149T3 (es) 2011-04-05
CN100381569C (zh) 2008-04-16
RU2005127869A (ru) 2006-02-10
KR20050106502A (ko) 2005-11-09
EP1602722B1 (en) 2011-01-26
EP1602722A1 (en) 2005-12-07
ATE496995T1 (de) 2011-02-15
CA2518049A1 (en) 2004-09-16
RU2316594C2 (ru) 2008-02-10
US20060019367A1 (en) 2006-01-26
EP1602722A4 (en) 2006-08-02
JP4482938B2 (ja) 2010-06-16
CA2518049C (en) 2013-05-07
US8105802B2 (en) 2012-01-31

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 24, 8O C/C 13, 10, 22 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.