BR112018009161A2 - limitação da trealose produzida por levedura em fermentação - Google Patents

limitação da trealose produzida por levedura em fermentação

Info

Publication number
BR112018009161A2
BR112018009161A2 BR112018009161-8A BR112018009161A BR112018009161A2 BR 112018009161 A2 BR112018009161 A2 BR 112018009161A2 BR 112018009161 A BR112018009161 A BR 112018009161A BR 112018009161 A2 BR112018009161 A2 BR 112018009161A2
Authority
BR
Brazil
Prior art keywords
production
trehalose
limitation
fermenting yeast
host cells
Prior art date
Application number
BR112018009161-8A
Other languages
English (en)
Inventor
Charles F. Rice
Ryan Skinner
Trisha Barrett
Aaron Argyros
Original Assignee
Lallemand Hungary Liquidity Management Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lallemand Hungary Liquidity Management Llc filed Critical Lallemand Hungary Liquidity Management Llc
Priority to BR122023025807-0A priority Critical patent/BR122023025807A2/pt
Publication of BR112018009161A2 publication Critical patent/BR112018009161A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01028—Alpha,alpha-trehalase (3.2.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente divulgação se refere a células hospedeiras de levedura recombinante que possuem (i) uma primeira modificação genética para reduzir a produção de uma ou mais enzimas nativas que funcionam para produzir glicerol ou regular a síntese de glicerol e/ou permitir a produção de uma glucoamilase heteróloga, e (ii) uma segunda modificação genética para reduzir a produção de uma ou mais enzimas nativas que funcionam para produzir trealose ou regular a síntese de trealose e/ou permitir a expressão de uma trealase heteróloga. as células hospedeiras de levedura recombinante podem ser utilizadas para limitar a produção de trealose (produzida por levedura) (especialmente trealose extracelular) durante a fermentação e, em algumas modalidades, podem aumentar a produção de um produto de fermentação (tal como, por exemplo, etanol).
BR112018009161-8A 2015-11-06 2016-11-04 limitação da trealose produzida por levedura em fermentação BR112018009161A2 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122023025807-0A BR122023025807A2 (pt) 2015-11-06 2016-11-04 Célula hospedeira de levedura recombinante e método para aumentar a produção de um produto de fermentação

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562251885P 2015-11-06 2015-11-06
US62/251,885 2015-11-06
PCT/IB2016/056658 WO2017077504A1 (en) 2015-11-06 2016-11-04 Limiting yeast-produced trehalose in fermentation

Publications (1)

Publication Number Publication Date
BR112018009161A2 true BR112018009161A2 (pt) 2019-04-16

Family

ID=57321370

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112018009161-8A BR112018009161A2 (pt) 2015-11-06 2016-11-04 limitação da trealose produzida por levedura em fermentação
BR122023025807-0A BR122023025807A2 (pt) 2015-11-06 2016-11-04 Célula hospedeira de levedura recombinante e método para aumentar a produção de um produto de fermentação

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR122023025807-0A BR122023025807A2 (pt) 2015-11-06 2016-11-04 Célula hospedeira de levedura recombinante e método para aumentar a produção de um produto de fermentação

Country Status (7)

Country Link
US (5) US10570421B2 (pt)
EP (1) EP3371317A1 (pt)
CN (1) CN108474010A (pt)
BR (2) BR112018009161A2 (pt)
CA (1) CA3004228A1 (pt)
MX (1) MX2018005692A (pt)
WO (1) WO2017077504A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3004228A1 (en) 2015-11-06 2017-05-11 Lallemand Hungary Liquidity Management Llc Limiting yeast-produced trehalose in fermentation
US11041218B2 (en) 2017-05-04 2021-06-22 Cargill, Incorporated Genetically modified trehalase-expressing yeasts and fermentation processes using such genetically modified yeasts
WO2019063542A1 (en) * 2017-09-29 2019-04-04 Dsm Ip Assets B.V. IMPROVED ETHANOL PRODUCTION WITHOUT GLYCEROL
EP3827088A1 (en) 2018-07-25 2021-06-02 Novozymes A/S Enzyme-expressing yeast for ethanol production
CN109182301B (zh) * 2018-08-17 2021-12-07 广东溢多利生物科技股份有限公司 一种二糖降解酶基因及其应用
WO2020057476A1 (zh) * 2018-09-17 2020-03-26 南京百斯杰生物工程有限公司 海藻糖酶在发酵生产中的应用
MX2021003955A (es) 2018-10-08 2021-05-27 Novozymes As Levadura que expresa enzimas para la produccion de etanol.
WO2020100072A1 (en) * 2018-11-13 2020-05-22 Lallemand Hungary Liquidity Management Llc Synergistic bacterial and yeast combinations
US20220220487A1 (en) * 2018-11-13 2022-07-14 Lallemand Hungary Liquidity Management Llc Combined expression of trehalose producing and trehalose degrading enzymes
US20220307036A1 (en) 2019-08-06 2022-09-29 Novozymes A/S Fusion proteins for improved enzyme expression
US20210163995A1 (en) 2019-11-29 2021-06-03 Lallemand Hungary Liquidity Management Llc Process for displacing an exogenous enzyme
BR112022010434A2 (pt) 2019-11-29 2022-10-11 Lallemand Hungary Liquidity Man Llc Levedura que expressa glucoamilase heteróloga
WO2021229429A2 (en) * 2020-05-11 2021-11-18 Lallemand Hungary Liquidity Management Llc Recombinant yeast host cell expressing an hydrolase
WO2022261003A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation
WO2023079048A1 (en) * 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
CN114350726A (zh) * 2022-01-11 2022-04-15 华南理工大学 一种以纤维素为底物合成海藻糖的方法
CN117568197A (zh) * 2023-11-20 2024-02-20 湖南农业大学 一种生产***素F2α的酵母工程菌及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0513437A (pt) * 2004-07-16 2008-05-06 Univ Delft Tech célula hospedeira eucariótica com a capacidade para isomerizar diretamente xilose em xilulose, e, processos para produzir etanol e um produto de fermentação
CA2782128C (en) * 2009-11-30 2019-01-15 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
EP2576762A4 (en) 2010-06-03 2013-12-04 Mascoma Corp YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE
CN103298932B (zh) * 2010-11-08 2016-08-10 诺维信公司 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸
BR112013025753A8 (pt) 2011-04-05 2018-06-12 Lallemand Hungary Liquidity Man Llc Métodos para aprimoramento do rendimento de produto e produção em um micro-organismo através da adição de aceptores de elétrons alternativos
BR112016009365A2 (pt) 2013-10-28 2017-09-19 Danisco Us Inc Método para aumentar, melhorar e estender a produção de etanol e método para diminuir as concentrações finais de dp2 em um produto de fermentação
US20160264927A1 (en) 2013-10-28 2016-09-15 Danisco Us Inc. Large Scale Genetically Engineered Active Dry Yeast
EP3122876B1 (en) * 2014-03-28 2020-11-25 Danisco US Inc. Altered host cell pathway for improved ethanol production
CA3004228A1 (en) * 2015-11-06 2017-05-11 Lallemand Hungary Liquidity Management Llc Limiting yeast-produced trehalose in fermentation
BR112022010434A2 (pt) * 2019-11-29 2022-10-11 Lallemand Hungary Liquidity Man Llc Levedura que expressa glucoamilase heteróloga

Also Published As

Publication number Publication date
US20180320203A1 (en) 2018-11-08
US11634734B2 (en) 2023-04-25
CN108474010A (zh) 2018-08-31
US20230265463A1 (en) 2023-08-24
US20200231991A1 (en) 2020-07-23
US20210163998A1 (en) 2021-06-03
US10947568B2 (en) 2021-03-16
BR122023025807A2 (pt) 2024-01-16
US10570421B2 (en) 2020-02-25
US20210163999A1 (en) 2021-06-03
MX2018005692A (es) 2019-03-14
US11572576B2 (en) 2023-02-07
WO2017077504A1 (en) 2017-05-11
CA3004228A1 (en) 2017-05-11
EP3371317A1 (en) 2018-09-12

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B154 Notification of filing of divisional application [chapter 15.50 patent gazette]

Free format text: O PEDIDO FOI DIVIDIDO NO BR122023025807-0 PROTOCOLO 870230108318 EM 07/12/2023 20:09.

B06A Patent application procedure suspended [chapter 6.1 patent gazette]