BR112021021777A2 - Métodos para a produção de ergotioneína - Google Patents

Métodos para a produção de ergotioneína

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Publication number
BR112021021777A2
BR112021021777A2 BR112021021777A BR112021021777A BR112021021777A2 BR 112021021777 A2 BR112021021777 A2 BR 112021021777A2 BR 112021021777 A BR112021021777 A BR 112021021777A BR 112021021777 A BR112021021777 A BR 112021021777A BR 112021021777 A2 BR112021021777 A2 BR 112021021777A2
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BR
Brazil
Prior art keywords
ergothioneine
production
methods
factories
yeast
Prior art date
Application number
BR112021021777A
Other languages
English (en)
Inventor
Darbani Shirvanehdeh Behrooz
Bruce Kell Douglas
Irina Borodina
Van Der Hoek Steven
Original Assignee
Univ Danmarks Tekniske
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Danmarks Tekniske filed Critical Univ Danmarks Tekniske
Publication of BR112021021777A2 publication Critical patent/BR112021021777A2/pt

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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01006—Methionine adenosyltransferase (2.5.1.6), i.e. adenosylmethionine synthetase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)

Abstract

métodos para a produção de ergotioneína. a presente invenção se refere a fábricas microbianas, em particular, fábricas de levedura, para a produção de ergotioneína. também são fornecidos métodos para produzir ergotioneína em uma célula de levedura, assim como ácidos nucleicos, polipeptídeos, vetores e células hospedeiras úteis.
BR112021021777A 2019-04-30 2020-04-29 Métodos para a produção de ergotioneína BR112021021777A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19171749 2019-04-30
PCT/EP2020/061866 WO2020221795A1 (en) 2019-04-30 2020-04-29 Methods for production of ergothioneine

Publications (1)

Publication Number Publication Date
BR112021021777A2 true BR112021021777A2 (pt) 2022-02-08

Family

ID=66334252

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021021777A BR112021021777A2 (pt) 2019-04-30 2020-04-29 Métodos para a produção de ergotioneína

Country Status (8)

Country Link
US (1) US20220220520A1 (pt)
EP (1) EP3963048A1 (pt)
KR (1) KR20220003035A (pt)
CN (1) CN113993989A (pt)
AU (1) AU2020266254A1 (pt)
BR (1) BR112021021777A2 (pt)
CA (1) CA3137619A1 (pt)
WO (1) WO2020221795A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114277042A (zh) * 2021-12-29 2022-04-05 内蒙古金达威药业有限公司 一种高产麦角硫因的圆红冬孢酵母重组表达菌株及其构建方法和应用
JP7450959B2 (ja) 2022-01-07 2024-03-18 シャメン オアミック バイオテック カンパニー リミテッド 酵母菌およびそのエルゴチオネイン製造における用途
CN115873886B (zh) * 2022-04-27 2023-11-10 武汉合生科技有限公司 生物合成麦角硫因的方法及载体
CN114854659B (zh) * 2022-05-27 2024-03-26 深圳中科欣扬生物科技有限公司 一种麦角硫因生产工艺及其应用
CN114854660B (zh) * 2022-05-27 2024-03-26 深圳中科欣扬生物科技有限公司 一种高产麦角硫因的基因工程菌
CN116286421B (zh) * 2023-03-23 2023-10-27 广州悦荟化妆品有限公司 一种用于生产麦角硫因的毕赤酵母菌株及其构建方法和应用
CN116926103B (zh) * 2023-07-14 2024-01-19 合曜生物科技(南京)有限公司 一种高产麦角硫因的圆红冬孢酵母工程菌的构建方法及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2016014235A (es) * 2014-04-29 2017-02-14 Conagen Inc Biosintesis microbiana de ergotioneina.
JP6263672B1 (ja) * 2016-02-29 2018-01-17 長瀬産業株式会社 エルゴチオネインの発酵生産

Also Published As

Publication number Publication date
WO2020221795A1 (en) 2020-11-05
CA3137619A1 (en) 2020-11-05
KR20220003035A (ko) 2022-01-07
AU2020266254A1 (en) 2021-12-23
CN113993989A (zh) 2022-01-28
US20220220520A1 (en) 2022-07-14
EP3963048A1 (en) 2022-03-09

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