BR112017000167A2 - dispositivo de microfluidos - Google Patents

dispositivo de microfluidos

Info

Publication number
BR112017000167A2
BR112017000167A2 BR112017000167-5A BR112017000167A BR112017000167A2 BR 112017000167 A2 BR112017000167 A2 BR 112017000167A2 BR 112017000167 A BR112017000167 A BR 112017000167A BR 112017000167 A2 BR112017000167 A2 BR 112017000167A2
Authority
BR
Brazil
Prior art keywords
fluid connection
fluid
channel
microfluidic device
cell channels
Prior art date
Application number
BR112017000167-5A
Other languages
English (en)
Other versions
BR112017000167B1 (pt
Inventor
Baltekin Özden
I. Andersson Dan
Original Assignee
Johan Elf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johan Elf filed Critical Johan Elf
Publication of BR112017000167A2 publication Critical patent/BR112017000167A2/pt
Publication of BR112017000167B1 publication Critical patent/BR112017000167B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Toxicology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)

Abstract

um dispositivo de microfluidos (1) compreende um substrato (10) transparente para a imagiologia e tem uma pluralidade de canais de células espacialmente definidos e separados (20) que tem uma dimensão para acomodar as células em monocamada. uma respectiva primeira extremidade (22) dos canais de células (20) está em ligação de fluido com um canal de entrada de fluxo (30) que tem uma primeira extremidade (32) em ligação de fluido com uma primeira porta de fluido (31) e uma segunda extremidade (34) em ligação de fluido com uma segunda porta de fluido (33). uma respectiva segunda extremidade (24) dos canais de células (20) está em ligação de fluido com uma primeira extremidade (42) de um canal respectivo de lavagem (40) que tem uma segunda extremidade (44) em ligação de fluido com um canal de saída de fluxo (50). o canal de saída de fluxo (50) está em conexão de fluido com uma terceira porta de fluido (51). os canais de lavagem (40) têm uma dimensão pequena demais para acomodar células.
BR112017000167-5A 2014-07-07 2015-06-12 Dispositivo de microfluidos, método de carregamento de dispositivo de microfluidos, método para testes de sensibilidade aos antibióticos, método para genotipagem de células in situ e método para a caracterização do fenótipo de células BR112017000167B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE1450860 2014-07-07
SE1450860-0 2014-07-07
WOPCT/SE2015/050227 2015-02-27
PCT/SE2015/050227 WO2016007063A1 (en) 2014-07-07 2015-02-27 Phenotypic characterization and in situ genotyping of a library of genetically different cells
PCT/SE2015/050685 WO2016007068A1 (en) 2014-07-07 2015-06-12 Microfluidic device

Publications (2)

Publication Number Publication Date
BR112017000167A2 true BR112017000167A2 (pt) 2018-01-16
BR112017000167B1 BR112017000167B1 (pt) 2021-08-24

Family

ID=55064558

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112017000168-3A BR112017000168B1 (pt) 2014-07-07 2015-02-27 Método e sistema de caracterização de uma biblioteca de uma pluralidade de linhagens de células de diferentes genótipos e possuindo regiões variáveis diferentes
BR112017000167-5A BR112017000167B1 (pt) 2014-07-07 2015-06-12 Dispositivo de microfluidos, método de carregamento de dispositivo de microfluidos, método para testes de sensibilidade aos antibióticos, método para genotipagem de células in situ e método para a caracterização do fenótipo de células

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR112017000168-3A BR112017000168B1 (pt) 2014-07-07 2015-02-27 Método e sistema de caracterização de uma biblioteca de uma pluralidade de linhagens de células de diferentes genótipos e possuindo regiões variáveis diferentes

Country Status (11)

Country Link
US (3) US10570437B2 (pt)
EP (2) EP3167061B1 (pt)
JP (3) JP6600349B2 (pt)
CN (2) CN106471122B (pt)
AU (2) AU2015288389B2 (pt)
BR (2) BR112017000168B1 (pt)
CA (2) CA2954360C (pt)
DK (1) DK3167044T3 (pt)
ES (1) ES2762914T3 (pt)
PL (1) PL3167044T3 (pt)
WO (2) WO2016007063A1 (pt)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106471122B (zh) * 2014-07-07 2020-07-03 阿斯特瑞格诊断公司 在遗传上不同的细胞的文库的表型鉴定及原位基因分型
US11046952B2 (en) 2015-03-16 2021-06-29 The Broad Institute, Inc. Encoding of DNA vector identity via iterative hybridization detection of a barcode transcript
CA3160383A1 (en) * 2015-05-11 2016-11-17 Illumina, Inc. Platform for discovery and analysis of therapeutic agents
EP3472359B1 (en) 2016-06-21 2022-03-16 10X Genomics, Inc. Nucleic acid sequencing
CA3057295A1 (en) * 2017-03-22 2018-09-27 Astrego Diagnostics Ab Phenotypic characterization of cells
EP3704058A4 (en) * 2017-10-31 2021-11-10 Astrego Diagnostics AB MICROFLUIDIC DEVICE FOR CELLULAR CHARACTERIZATION
US11527324B2 (en) 2017-11-10 2022-12-13 Reliant Immune Diagnostics, Inc. Artificial intelligence response system based on testing with parallel/serial dual microfluidic chip
US11124821B2 (en) 2017-11-10 2021-09-21 Reliant Immune Diagnostics, Inc. Microfluidic testing system with cell capture/analysis regions for processing in a parallel and serial manner
US11041185B2 (en) 2017-11-10 2021-06-22 Reliant Immune Diagnostics, Inc. Modular parallel/serial dual microfluidic chip
US11200986B2 (en) 2017-11-10 2021-12-14 Reliant Immune Diagnostics, Inc. Database and machine learning in response to parallel serial dual microfluidic chip
US10930380B2 (en) 2017-11-10 2021-02-23 Reliant Immune Diagnostics, Inc. Communication loop and record loop system for parallel/serial dual microfluidic chip
US10930381B2 (en) 2017-11-10 2021-02-23 Reliant Immune Diagnostics, Inc. Microfluidic testing system for mobile veterinary applications
JP7335249B2 (ja) * 2018-02-16 2023-08-29 アストレゴ ダイアグノスティクス エービー 微小流体装置での細胞捕捉
CN111615630B (zh) * 2018-02-16 2023-09-15 阿斯特瑞格诊断公司 微流体装置
JP7282327B2 (ja) * 2018-08-23 2023-05-29 株式会社デンソー 細胞固定デバイス、細胞固定方法、及び細胞操作方法
SE542462C2 (en) 2018-09-20 2020-05-12 Astrego Diagnostics Ab Sample loading cartridge for a microfluidic device
US11421273B2 (en) * 2018-12-03 2022-08-23 California Institute Of Technology In situ readout of DNA barcodes
CN109580485A (zh) * 2019-01-18 2019-04-05 大连理工大学 一种用于红细胞超分辨成像的固定装置和使用方法
AU2020354648A1 (en) * 2019-09-26 2022-04-14 Jumpcode Genomics, Inc. Method and system for targeted nucleic acid sequencing
WO2022197490A1 (en) * 2021-03-16 2022-09-22 Scale Biosciences Inc. Method for adding spatially-addressable barcodes to nucleic acids of a cellular sample in situ
EP4234087B1 (en) 2022-02-24 2024-07-03 Sysmex Astrego AB Cartridge and method of analysing a biological sample

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2666849A3 (en) 2002-04-01 2014-05-28 Fluidigm Corporation Microfluidic particle-analysis systems
DE60328392D1 (de) * 2002-04-30 2009-08-27 Gyros Patent Ab Mittels einer integrierten mikrofluidischen vorrichtung
WO2003093802A1 (en) 2002-04-30 2003-11-13 Gyros Ab Integrated microfluidic device (ea)
CN101165161B (zh) * 2007-07-27 2013-10-30 中国科学院上海微***与信息技术研究所 一种微流体浓度梯度细胞培养芯片及其制备方法和应用
EP2178641B1 (en) * 2007-08-09 2018-04-11 Progenity, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
FR2937050A1 (fr) 2008-10-10 2010-04-16 Inst Curie Dispositif de culture cellulaire
EP2350652A2 (en) * 2008-10-10 2011-08-03 Cnrs-Dae Cell sorting device
US20100102007A1 (en) 2008-10-23 2010-04-29 Abdel Magid Kalo Process for Treatment of Sewage Waste Water
US8936762B2 (en) * 2009-09-01 2015-01-20 Trustees Of Boston University High throughput multichannel reader and uses thereof
CN101705179A (zh) * 2009-12-13 2010-05-12 周小棉 肿瘤细胞耐药性检测芯片
US20130115606A1 (en) * 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
KR101720378B1 (ko) 2010-09-29 2017-03-28 더 브리검 앤드 우먼즈 하스피털, 인크. 세포 상호작용의 고 처리량 조사를 위한 장치
CA2826131C (en) * 2011-02-02 2019-11-05 Jay Ashok Shendure Massively parallel continguity mapping
KR101391679B1 (ko) 2011-07-15 2014-05-07 도꾸리쯔교세이호징 가가꾸 기쥬쯔 신꼬 기꼬 세포 배양 장치, 세포 배양 장기 관찰 장치, 세포 장기 배양 방법, 및 세포 배양 장기 관찰 방법
US9957472B2 (en) 2011-09-22 2018-05-01 Georgia Tech Research Corporation Deterministic high-density single-cell trap array
EP2782866A4 (en) * 2011-11-23 2015-08-05 Univ Toronto DEVICES AND METHODS FOR PRODUCING FLAT POLYMERIC MATERIALS USING MICROFLUIDIC DEVICES
US9149806B2 (en) * 2012-01-10 2015-10-06 Biopico Systems Inc Microfluidic devices and methods for cell sorting, cell culture and cells based diagnostics and therapeutics
US9494568B2 (en) 2012-02-03 2016-11-15 Neobios Pte Ltd Passive microfluidic device and a method of forming the same
EP3285062B1 (en) 2012-02-29 2019-08-07 Fluidigm Corporation Methods, systems, and devices for multiple single-cell capturing and processing using microfluidics
CA2868117C (en) * 2012-05-08 2021-08-10 Cellecta, Inc. Clonal analysis of functional genomic assays and compositions for practicing same
US10168317B2 (en) 2012-06-08 2019-01-01 Cornell University Microfluidic device, system, and method for tracking single cells and single cell lineages
JP6401700B2 (ja) 2012-06-29 2018-10-10 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
CN102796667B (zh) * 2012-08-21 2014-07-30 北京大学 一种可布置不同细胞密度的微流控芯片及其应用
CA2894511C (en) * 2012-12-11 2021-12-07 Albert Einstein College Of Medicine Of Yeshiva University Methods for high throughput receptor:ligand identification
WO2014152048A2 (en) * 2013-03-14 2014-09-25 Cytonome/St, Llc Assemblies and methods for reducing optical crosstalk in particle processing systems
CN106471122B (zh) * 2014-07-07 2020-07-03 阿斯特瑞格诊断公司 在遗传上不同的细胞的文库的表型鉴定及原位基因分型

Also Published As

Publication number Publication date
US20170159048A1 (en) 2017-06-08
CA2954360C (en) 2022-11-22
EP3167044A1 (en) 2017-05-17
DK3167044T3 (da) 2019-12-02
CN106471122B (zh) 2020-07-03
US10913969B2 (en) 2021-02-09
JP2020054365A (ja) 2020-04-09
JP7066206B2 (ja) 2022-05-13
EP3167061B1 (en) 2019-02-20
EP3167061A4 (en) 2018-02-28
CN106661530B (zh) 2021-06-04
BR112017000167B1 (pt) 2021-08-24
US20170137861A1 (en) 2017-05-18
US20180320216A1 (en) 2018-11-08
CA2954360A1 (en) 2016-01-14
AU2015288329A1 (en) 2017-01-12
CA2954378A1 (en) 2016-01-14
EP3167061A1 (en) 2017-05-17
CN106661530A (zh) 2017-05-10
JP6629287B2 (ja) 2020-01-15
US10570437B2 (en) 2020-02-25
ES2762914T3 (es) 2020-05-26
JP6600349B2 (ja) 2019-10-30
AU2015288389A1 (en) 2017-01-12
WO2016007068A1 (en) 2016-01-14
EP3167044B1 (en) 2019-10-09
CA2954378C (en) 2022-11-22
AU2015288329B2 (en) 2020-07-23
BR112017000168A2 (pt) 2018-01-16
PL3167044T3 (pl) 2020-08-24
BR112017000168B1 (pt) 2023-04-18
AU2015288389B2 (en) 2021-05-13
JP2017520262A (ja) 2017-07-27
US10041104B2 (en) 2018-08-07
EP3167044A4 (en) 2018-06-20
JP2017519517A (ja) 2017-07-20
WO2016007063A1 (en) 2016-01-14
CN106471122A (zh) 2017-03-01

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Owner name: ASTREGO DIAGNOSTICS AB (SE)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/06/2015, OBSERVADAS AS CONDICOES LEGAIS.