BR0009420A - Método de detecção de diluição simples e canal único - Google Patents
Método de detecção de diluição simples e canal únicoInfo
- Publication number
- BR0009420A BR0009420A BR0009420-0A BR0009420A BR0009420A BR 0009420 A BR0009420 A BR 0009420A BR 0009420 A BR0009420 A BR 0009420A BR 0009420 A BR0009420 A BR 0009420A
- Authority
- BR
- Brazil
- Prior art keywords
- single channel
- whole blood
- sample
- blood
- detection
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 239000012895 dilution Substances 0.000 title abstract 2
- 238000010790 dilution Methods 0.000 title abstract 2
- 210000004369 blood Anatomy 0.000 abstract 4
- 239000008280 blood Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 238000004458 analytical method Methods 0.000 abstract 1
- 210000000601 blood cell Anatomy 0.000 abstract 1
- 238000004820 blood count Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000003113 dilution method Methods 0.000 abstract 1
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 238000000684 flow cytometry Methods 0.000 abstract 1
- 210000000265 leukocyte Anatomy 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 210000001995 reticulocyte Anatomy 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Ecology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dispersion Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
Abstract
"MéTODO DE DETECçãO DE DILUIçãO SIMPLES E CANAL úNICO". A invenção fornece um método e sistema de diluição simples para identificar, analisar e quantificar os componentes celulares do sangue integral utilizando um único canal de um analisador de hematologia automatizado utilizando citometria de fluxo e a detecção da luz espalhada e absorvida por cada célula. O método envolve a utilização de um corante orgânico na solução reagente para tingir o ácido nucleico de reticulócitos, incluindo plaquetas reticuladas, e células de sangue brancas na amostra. O método de canal único é particularmente útil para determinar contagens de hemácias e avaliar parâmetros de uma amostra de sangue integral, para amostras de sangue tanto humanos como de mamíferos não-humanos. O aparelho para efetuar o método fornece um analisador económico, compacto e economizador de espaço para realizar a analise completa de células sang³íneas em uma amostra de sangue integral de mamífero.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12720999P | 1999-03-31 | 1999-03-31 | |
PCT/US2000/008512 WO2000058727A1 (en) | 1999-03-31 | 2000-03-31 | Single channel, single dilution detection method |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0009420A true BR0009420A (pt) | 2002-04-09 |
Family
ID=22428869
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0009420-0A BR0009420A (pt) | 1999-03-31 | 2000-03-31 | Método de detecção de diluição simples e canal único |
Country Status (8)
Country | Link |
---|---|
US (1) | US6524858B1 (pt) |
EP (1) | EP1166110A1 (pt) |
JP (1) | JP2002540426A (pt) |
AU (1) | AU775260B2 (pt) |
BR (1) | BR0009420A (pt) |
CA (1) | CA2367780A1 (pt) |
IL (1) | IL145669A0 (pt) |
WO (1) | WO2000058727A1 (pt) |
Families Citing this family (56)
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JP2003010101A (ja) * | 2001-04-27 | 2003-01-14 | Fuji Photo Film Co Ltd | 内視鏡装置の撮像方法および装置 |
US6706526B2 (en) * | 2002-01-24 | 2004-03-16 | Coulter International Corp. | Low formaldehyde producing blood diluent |
KR20050118668A (ko) * | 2003-01-21 | 2005-12-19 | 마이크로닉스 인코포레이티드. | 유체의 미세유체식 조작, 증폭 및 분석, 예를 들어 세균검정 및 항글로불린 시험을 위한 방법 및 시스템 |
US20040228765A1 (en) * | 2003-05-14 | 2004-11-18 | Witty Thomas R. | Point of care diagnostic platform |
US20040228766A1 (en) * | 2003-05-14 | 2004-11-18 | Witty Thomas R. | Point of care diagnostic platform |
EP1500932A1 (en) * | 2003-07-21 | 2005-01-26 | Michael J. Sommer | A lysing reagent for the analysis and enumeration of residual white blood cells in leukocyte-reduced blood banking products suitable for the use in an automated clinical analyzer |
US7095492B2 (en) * | 2003-12-19 | 2006-08-22 | Beckman Coulter, Inc. | Method and apparatus for measuring cell-by-cell hemoglobin |
JP4417143B2 (ja) * | 2004-03-11 | 2010-02-17 | シスメックス株式会社 | 試料分析装置、プログラムおよびそのプログラムを記録した記録媒体 |
WO2006014958A2 (en) * | 2004-07-26 | 2006-02-09 | Bayer Healthcare Llc | Platelet activation markers as predictors of disease and of response to therapy and for monitoring therapeutic progress |
FR2883972B1 (fr) * | 2005-03-31 | 2007-11-16 | C2 Diagnostics Sa | Procede pour l'analyse d'un echantillon de sang et appareil et reactif pour sa mise en oeuvre |
JP4848555B2 (ja) * | 2005-04-19 | 2011-12-28 | 関東化学株式会社 | 尿用防腐剤組成物 |
US7344890B2 (en) * | 2005-11-09 | 2008-03-18 | Beckman Coulter, Inc. | Method for discriminating platelets from red blood cells |
US7804594B2 (en) | 2006-12-29 | 2010-09-28 | Abbott Laboratories, Inc. | Method and apparatus for rapidly counting and identifying biological particles in a flow stream |
JP4926812B2 (ja) * | 2007-02-01 | 2012-05-09 | シスメックス株式会社 | 血球分析装置および体液分析方法 |
CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
US8102161B2 (en) * | 2007-09-25 | 2012-01-24 | Tdk Corporation | Stable output in a switching power supply by smoothing the output of the secondary coil |
US8159670B2 (en) * | 2007-11-05 | 2012-04-17 | Abbott Laboratories | Method and apparatus for rapidly counting and identifying biological particles in a flow stream |
CN105440725A (zh) * | 2008-01-04 | 2016-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料,组合物及在生物样品染色中的用途 |
CN101602762B (zh) * | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
FR2933192B1 (fr) * | 2008-06-25 | 2010-09-17 | Horiba Abx Sas | Dispositif et procede de mesure electro optique destines a la classification et au comptage d'elements microscopiques. |
CN101726579B (zh) * | 2008-10-17 | 2014-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液检测试剂和方法 |
JP5203889B2 (ja) * | 2008-10-28 | 2013-06-05 | シスメックス株式会社 | 検体分析装置及び検体分析方法 |
US8367358B2 (en) * | 2008-12-17 | 2013-02-05 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Reagent, kit and method for differentiating and counting leukocytes |
JP4969596B2 (ja) * | 2009-02-04 | 2012-07-04 | シスメックス株式会社 | 試料分析装置、試料分析方法およびプログラム |
JP5871792B2 (ja) * | 2009-04-27 | 2016-03-01 | アボット・ラボラトリーズAbbott Laboratories | 自動血液分析器において、レーザーからの前方散乱を用いることによる、赤血球細胞を白血球細胞から判別する方法 |
CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
US8911669B2 (en) | 2009-08-24 | 2014-12-16 | Abbott Laboratories | Method for flagging a sample |
US8538499B2 (en) * | 2009-09-23 | 2013-09-17 | Lightouch Medical, Inc. | Process and apparatus for non-invasive, continuous in vivo measurement of hematocrit |
US8906308B2 (en) | 2010-01-15 | 2014-12-09 | Abbott Laboratories | Method for determining volume and hemoglobin content of individual red blood cells |
FR2956207B1 (fr) * | 2010-02-10 | 2012-05-04 | Horiba Abx Sas | Dispositif et procede de mesures multiparametriques de microparticules dans un fluide |
US9097704B2 (en) | 2010-05-05 | 2015-08-04 | Abbott Laboratories | Method for hematology analysis |
CN101846626B (zh) * | 2010-05-24 | 2012-07-11 | 天津商业大学 | 一种利用流式细胞仪检测腹腔吞噬细胞活性的方法 |
US9091677B2 (en) | 2010-08-09 | 2015-07-28 | Beckman Coulter, Inc. | Isotonic buffered composition and method that enables counting of cells |
JP5868982B2 (ja) | 2010-09-16 | 2016-02-24 | ザ ジェネラル ホスピタル コーポレイション | 診断のための赤血球動力学 |
US9005909B2 (en) | 2011-01-06 | 2015-04-14 | Rigel Pharmaceuticals, Inc. | Whole blood assay for measuring AMPK activation |
CN102279147B (zh) * | 2011-03-08 | 2012-12-19 | 山东大学威海分校 | 刺参体腔细胞吞噬活性的流式细胞检测方法 |
US10512669B2 (en) | 2011-06-02 | 2019-12-24 | The Regents Of The University Of California | Blockade of inflammatory proteases with cyclic peptides |
WO2014008424A1 (en) * | 2012-07-05 | 2014-01-09 | Beckman Coulter, Inc. | Method and apparatus for determining white blood cell counts |
CN104903699A (zh) * | 2012-12-31 | 2015-09-09 | 贝克曼考尔特公司 | 未成熟血小板的计数***和方法 |
DE102013203839B4 (de) | 2013-03-06 | 2014-10-16 | Siemens Healthcare Diagnostics Products Gmbh | Verfahren zur Analyse einer Blutprobe und zugehöriges Hämatologie-System |
EP2972315B1 (en) * | 2013-03-12 | 2020-01-15 | Abbott Laboratories | System for optical analysis of the mean corpuscular volume of red blood cells |
DE102013216362A1 (de) | 2013-08-19 | 2015-02-19 | Siemens Healthcare Diagnostics Products Gmbh | Analyseverfahren zur Klassifikationsunterstützung |
WO2016043683A1 (en) * | 2014-09-18 | 2016-03-24 | Ciftci Ihsan Hakki | A blood count device |
CA2980133A1 (en) | 2015-03-25 | 2016-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Single cell analysis using secondary ion mass spectrometry |
EP3076333A1 (de) | 2015-04-02 | 2016-10-05 | Siemens Aktiengesellschaft | Analyseverfahren zur klassifikation einer testdispersion hinsichtlich des vorliegens einer sichelzellenanämie |
US10955423B2 (en) | 2015-12-15 | 2021-03-23 | The General Hospital Corporation | Methods of estimating blood glucose and related systems |
US10024780B2 (en) * | 2015-12-18 | 2018-07-17 | Abbott Laboratories (Diagnostics Division) | Methods for detecting events in a flow cytometer |
CN108463722B (zh) * | 2016-03-30 | 2021-09-21 | 西门子医疗保健诊断公司 | 用于处理血小板细胞数据的***、方法和设备 |
US11293852B2 (en) | 2016-04-07 | 2022-04-05 | The General Hospital Corporation | White blood cell population dynamics |
US11162945B2 (en) | 2016-04-11 | 2021-11-02 | Board Of Regents, The University Of Texas System | Methods and compositions for detecting single T cell receptor affinity and sequence |
EP3452825A4 (en) * | 2016-05-04 | 2020-01-08 | Labthroughput LLC | SYSTEM AND METHOD FOR DISTINGUISHING BLOOD COMPONENTS |
CN108303363B (zh) * | 2017-01-12 | 2023-08-22 | 长春市布拉泽医疗科技有限公司 | 一种血细胞分析方法及使用该方法的血细胞分析仪 |
ES2837438T3 (es) * | 2017-09-29 | 2021-06-30 | Siemens Healthcare Gmbh | Detección específica de malaria con microscopía óptica digital |
WO2019206298A1 (zh) * | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | 试剂、分析血小板的方法及血液细胞分析仪 |
WO2019206267A1 (zh) * | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | 光学检测***、血细胞分析仪及血小板检测方法 |
CN113495167B (zh) * | 2021-06-24 | 2024-05-03 | 迈克医疗电子有限公司 | 血细胞分析仪的试剂加载方法及装置 |
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US4727020A (en) | 1985-02-25 | 1988-02-23 | Becton, Dickinson And Company | Method for analysis of subpopulations of blood cells |
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JP3467310B2 (ja) * | 1994-04-21 | 2003-11-17 | シスメックス株式会社 | 白血球分析用試薬及び白血球の分類方法 |
US5888752A (en) * | 1995-05-16 | 1999-03-30 | Bayer Corporation | Universal rinse reagent and method for use in hematological analyses of whole blood samples |
US5817519A (en) * | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
US5830764A (en) * | 1996-11-12 | 1998-11-03 | Bayer Corporation | Methods and reagent compositions for the determination of membrane surface area and sphericity of erythrocytes and reticulocytes for the diagnosis of red blood cell disorders |
US6114173A (en) * | 1997-04-03 | 2000-09-05 | Bayer Corporation | Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood |
US6197593B1 (en) * | 1998-10-20 | 2001-03-06 | Coulter International Corp. | Method for enumerating blood cells |
US6060322A (en) * | 1998-10-20 | 2000-05-09 | Coulter International Corp. | Method for identification of reticulated cells |
-
2000
- 2000-03-31 EP EP00919919A patent/EP1166110A1/en not_active Withdrawn
- 2000-03-31 AU AU40531/00A patent/AU775260B2/en not_active Ceased
- 2000-03-31 JP JP2000608174A patent/JP2002540426A/ja active Pending
- 2000-03-31 WO PCT/US2000/008512 patent/WO2000058727A1/en not_active Application Discontinuation
- 2000-03-31 BR BR0009420-0A patent/BR0009420A/pt not_active Application Discontinuation
- 2000-03-31 IL IL14566900A patent/IL145669A0/xx unknown
- 2000-03-31 US US09/539,568 patent/US6524858B1/en not_active Expired - Lifetime
- 2000-03-31 CA CA002367780A patent/CA2367780A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1166110A1 (en) | 2002-01-02 |
AU775260B2 (en) | 2004-07-29 |
AU4053100A (en) | 2000-10-16 |
JP2002540426A (ja) | 2002-11-26 |
WO2000058727A1 (en) | 2000-10-05 |
US6524858B1 (en) | 2003-02-25 |
IL145669A0 (en) | 2002-06-30 |
CA2367780A1 (en) | 2000-10-05 |
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FA10 | Dismissal: dismissal - article 33 of industrial property law | ||
B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |