ATE481499T1 - Cocktail von nukleaseinhibitoren - Google Patents

Cocktail von nukleaseinhibitoren

Info

Publication number
ATE481499T1
ATE481499T1 AT00966924T AT00966924T ATE481499T1 AT E481499 T1 ATE481499 T1 AT E481499T1 AT 00966924 T AT00966924 T AT 00966924T AT 00966924 T AT00966924 T AT 00966924T AT E481499 T1 ATE481499 T1 AT E481499T1
Authority
AT
Austria
Prior art keywords
nuclease
antibodies
inhibitors
antibody
nuclease inhibitors
Prior art date
Application number
AT00966924T
Other languages
English (en)
Inventor
Matthew Winkler
W Kudlicki
Brittan Pasloske
Original Assignee
Ambion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Application granted granted Critical
Publication of ATE481499T1 publication Critical patent/ATE481499T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Alcoholic Beverages (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
AT00966924T 1999-09-24 2000-09-25 Cocktail von nukleaseinhibitoren ATE481499T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15587499P 1999-09-24 1999-09-24
PCT/US2000/026485 WO2001021830A1 (en) 1999-09-24 2000-09-25 Nuclease inhibitor cocktail

Publications (1)

Publication Number Publication Date
ATE481499T1 true ATE481499T1 (de) 2010-10-15

Family

ID=22557124

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00966924T ATE481499T1 (de) 1999-09-24 2000-09-25 Cocktail von nukleaseinhibitoren

Country Status (5)

Country Link
US (3) US6664379B1 (de)
EP (1) EP1220944B1 (de)
AT (1) ATE481499T1 (de)
DE (1) DE60044977D1 (de)
WO (1) WO2001021830A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
US6664379B1 (en) * 1999-09-24 2003-12-16 Ambion, Inc. Nuclease inhibitor cocktail
EP1373570B1 (de) * 2001-03-08 2007-09-12 Invitrogen Corporation Verbessertes in-vitro-synthesesystem
EP1300465A1 (de) * 2001-10-08 2003-04-09 Agrobiogen GmbH Biotechnologie Verfahren und Vorrichtung zur Isolierung von RNS-Proben
US20030170617A1 (en) 2002-01-28 2003-09-11 Pasloske Brittan L. Crude biological derivatives competent for nucleic acid detection
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
ES2579803T3 (es) 2002-09-03 2016-08-16 Quanta Biosciences, Inc. Composiciones y métodos mejorados para la síntesis de ADNc
ES2784011T3 (es) 2002-10-16 2020-09-21 Streck Inc Procedimiento y dispositivo para recoger y preservar las células para su análisis
US7291459B2 (en) * 2002-12-10 2007-11-06 University Of Alabama At Huntsville Nucleic acid detector and method of detecting targets within a sample
WO2004076640A2 (en) * 2003-02-25 2004-09-10 Ambion, Inc. Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same
CN1882688B (zh) † 2003-07-25 2014-07-16 安比恩股份有限公司 用于分离小rna分子的方法和组合物
US20050208501A1 (en) 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US8460684B2 (en) 2004-03-19 2013-06-11 Wisconsin Alumni Research Foundation Nuclease inhibitors and methods for their use
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2006051552A2 (en) * 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
DE102005015005A1 (de) * 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
US20100099149A1 (en) 2006-10-06 2010-04-22 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
AU2009301505A1 (en) * 2008-10-06 2010-04-15 Symphogen A/S Method for identifying and selecting drug candidates for combinatorial drug products
EP2175020A1 (de) * 2008-10-13 2010-04-14 Roche Diagnostics GmbH Verringerung der RNase-Aktivität in komplexen flüssigen Proben
US20110207667A1 (en) * 2008-10-30 2011-08-25 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Efficient expression of truncated human rnaset2 in e. coli
EP2367954B1 (de) * 2008-12-19 2015-02-18 Life Technologies Corporation Proteinase k inhibitore, verfahren und zusammensetzungen davon
US11634747B2 (en) * 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
WO2010096323A1 (en) 2009-02-18 2010-08-26 Streck, Inc. Preservation of cell-free nucleic acids
WO2011008553A1 (en) * 2009-06-29 2011-01-20 Life Technologies Corporation Rna sample stabilization in the presence of a transitional metal
EP3103883A1 (de) 2009-11-09 2016-12-14 Streck, Inc. Stabilisierung von rna in und extraktion aus intakten zellen in einer blutprobe
CN102243154B (zh) * 2011-04-02 2016-05-11 中山大学附属第一医院 一种周围神经切片染色的方法
US9956281B2 (en) 2011-05-04 2018-05-01 Streck, Inc. Inactivated virus compositions and methods of preparing such compositions
EP3150702B1 (de) 2011-06-19 2021-05-19 DNA Genotek, Inc. Vorrichtungen, lösungen und verfahren zur probenentnahme
WO2013055898A1 (en) * 2011-10-14 2013-04-18 The Trustees Of The University Of Pennsylvania Methods for enhancing the efficacy of microrna mediated interference
CN114134029A (zh) 2012-02-13 2022-03-04 纽莫德克斯莫勒库拉尔公司 用于处理和检测核酸的微流体盒
US11485968B2 (en) 2012-02-13 2022-11-01 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US11931740B2 (en) 2012-02-13 2024-03-19 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
JP6236864B2 (ja) * 2012-06-11 2017-11-29 東ソー株式会社 癌の検出方法及び膵臓特異的リボヌクレアーゼ１を認識する抗体
BR112015005718A2 (pt) * 2012-09-19 2017-07-04 Beckman Coulter Inc uso de íons divalentes, proteases, detergentes, e ph baixo na extração de ácidos nucleicos
ES2741749T3 (es) * 2012-10-25 2020-02-12 Neumodx Molecular Inc Método y materiales para el aislamiento de materiales de ácido nucleico
ES2938048T3 (es) 2013-07-24 2023-04-04 Streck Llc Composiciones y procedimientos para estabilizar las células tumorales circulantes
WO2015120406A1 (en) 2014-02-07 2015-08-13 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
EP3825411A1 (de) 2014-06-18 2021-05-26 Clear Gene, Inc. Verfahren, zusammensetzungen und vorrichtungen zur schnellanalyse von biologischen markern
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
EP4289356A3 (de) 2015-09-09 2024-02-28 Drawbridge Health, Inc. Vorrichtungen zur probeentnahme, stabilisierung und konservierung
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
WO2017106790A1 (en) 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
US11506655B2 (en) 2016-07-29 2022-11-22 Streck, Inc. Suspension composition for hematology analysis control
US20230272465A1 (en) * 2020-07-13 2023-08-31 The Board Of Trustees Of The Leland Stanford Junior University Systems and Methods to Enhance RNA Transcription and Uses Thereof
CN114249829B (zh) * 2021-12-20 2022-09-20 南京诺唯赞生物科技股份有限公司 一种RNase抑制剂的单克隆抗体及其制备方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58116498A (ja) 1981-12-28 1983-07-11 Takeda Chem Ind Ltd Ｉｌ‐２をコードする新規伝令ｒｎａの製造法
US5278062A (en) 1987-04-03 1994-01-11 Amgen Proteolytic enzymes
US5151504A (en) * 1989-11-17 1992-09-29 E. R. Squibb & Sons, Inc. Method for purification of monoclonal antibodies
US5470971A (en) 1991-03-11 1995-11-28 The University Of Medicine And Dentistry Of New Jersey Stress-induced proteins, genes coding therefor, transformed cells of organisms, methods and applications
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US6110968A (en) * 1995-12-26 2000-08-29 The Picower Institute For Medical Research Methods for treatment predicated on the presence of advanced glycosylation endproducts in tobacco and its combustion byproducts
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
DE69723430T2 (de) 1996-05-02 2004-04-15 The Procter & Gamble Company, Cincinnati Topische zusammensetzung mit dispergiertem tensidkomplex
WO1997040817A1 (en) 1996-05-02 1997-11-06 The Procter & Gamble Company Topical compositions comprising dispersed surfactant complex
US5968784A (en) 1997-01-15 1999-10-19 Chugai Pharmaceutical Co., Ltd. Method for analyzing quantitative expression of genes
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
US6869604B1 (en) * 1998-03-27 2005-03-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinant anti-tumor RNAse
US7264932B2 (en) 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
US6664379B1 (en) 1999-09-24 2003-12-16 Ambion, Inc. Nuclease inhibitor cocktail
US6793019B2 (en) 2002-07-10 2004-09-21 Abb Offshore Systems, Inc. Tapered ramp positive lock latch mechanism
US7021540B2 (en) * 2002-07-30 2006-04-04 Canon Kabushiki Kaisha Storage medium mounting/dismounting mechanism and information processing apparatus
WO2004076640A2 (en) 2003-02-25 2004-09-10 Ambion, Inc. Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same

Also Published As

Publication number Publication date
WO2001021830A1 (en) 2001-03-29
US20070148174A1 (en) 2007-06-28
US7445901B2 (en) 2008-11-04
US20050026284A1 (en) 2005-02-03
EP1220944B1 (de) 2010-09-15
EP1220944A4 (de) 2005-06-22
DE60044977D1 (de) 2010-10-28
US6664379B1 (en) 2003-12-16
EP1220944A1 (de) 2002-07-10
US7163793B2 (en) 2007-01-16

Similar Documents

Publication Publication Date Title
ATE481499T1 (de) Cocktail von nukleaseinhibitoren
ATE549414T1 (de) Verfahren zur isolierung von zellen
DK1421389T3 (da) Neurofibrilmarkører
EP2336187B8 (de) Zusammensetzungen und Verfahren von monoklonalen oder polyklonalen Antikörpern, die spezifisch für T-Zell Subpopulationen sind
DK0988307T3 (da) Fast fase-isolering af nucleinsyre
WO2005026209A3 (en) Monoclonal antibodies against hmgb1
PT2305715T (pt) Anticorpo monoclonal para a proteína de ligação a osteoprotegerina
CR9969A (es) Anticuerpos anti-globulomero ab, porciones de union a antigeno de estos, hibridomas correspondientes, acidos nucleicos, vectores, celulas huesped, metodos para producir dichos anticuerpos, composiciones que comprenden dichos anticuerpos
ATE412432T1 (de) Internalisierende erbb2 antikörper
ATE519863T1 (de) Nicht-fluoreszente energieübertragung
YU28503A (sh) Humanizovana anti-lt-beta-r antitela
EA199700087A1 (ru) Способ ингибирования катепсина к
EP2186884A3 (de) Monoklonale Antikörpergegen HCV Kernantigen
ATE536552T1 (de) Kompetitven differential screening
WO2002027031A3 (en) Methods and reagents for live-cell gene expression quantification
DE50115166D1 (de) System zur einfachen Nukleinsäureanalytik
DK0709455T3 (da) Monoklonalt antistof med humanglicentin, hybridoma til fremstilling af antistoffet og assaymetode for humanglicentin ved an
AU2003283240A1 (en) Antibody for isolating and/or identifying mesenchymal stem cells and method for isolating and/or identifying mesenchymal stem cells
CA2419046A1 (en) Method for detecting or measuring hbv
SE0002835D0 (sv) Method and kit for production of monoclonal antibodies
CO5300499A1 (es) Ensayo de inmunoabsorbente ligado a enzima de inhibicion (elisa) para deteccion y cuantificacion de anticuerpos en muestras biologicas
DK1161522T3 (da) Fremgangsmåde til isolering af CD8+-celler og beslægtede hybridomaceller, antistoffer og polypeptider
DE59813125D1 (de) Srcr domäne-enthaltendes protein
DE69227776D1 (de) Monoklonale antikörper gegen mutmassliche hepatitis c-virus ns5-proteine und ihre anwendungsmethoden
BR0204612A (pt) Anticorpos monoclonais antinúcleo de hcv

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties