DE60044977D1 - Cocktail von nukleaseinhibitoren - Google Patents

Cocktail von nukleaseinhibitoren

Info

Publication number
DE60044977D1
DE60044977D1 DE60044977T DE60044977T DE60044977D1 DE 60044977 D1 DE60044977 D1 DE 60044977D1 DE 60044977 T DE60044977 T DE 60044977T DE 60044977 T DE60044977 T DE 60044977T DE 60044977 D1 DE60044977 D1 DE 60044977D1
Authority
DE
Germany
Prior art keywords
nuclease
antibodies
inhibitors
antibody
nuclease inhibitors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60044977T
Other languages
English (en)
Inventor
Matthew M Winkler
Antoni W Kudlicki
Brittan L Pasloske
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ambion Inc
Original Assignee
Ambion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Application granted granted Critical
Publication of DE60044977D1 publication Critical patent/DE60044977D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Alcoholic Beverages (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
DE60044977T 1999-09-24 2000-09-25 Cocktail von nukleaseinhibitoren Expired - Lifetime DE60044977D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15587499P 1999-09-24 1999-09-24
PCT/US2000/026485 WO2001021830A1 (en) 1999-09-24 2000-09-25 Nuclease inhibitor cocktail

Publications (1)

Publication Number Publication Date
DE60044977D1 true DE60044977D1 (de) 2010-10-28

Family

ID=22557124

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60044977T Expired - Lifetime DE60044977D1 (de) 1999-09-24 2000-09-25 Cocktail von nukleaseinhibitoren

Country Status (5)

Country Link
US (3) US6664379B1 (de)
EP (1) EP1220944B1 (de)
AT (1) ATE481499T1 (de)
DE (1) DE60044977D1 (de)
WO (1) WO2001021830A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
DE60044977D1 (de) * 1999-09-24 2010-10-28 Ambion Inc Cocktail von nukleaseinhibitoren
EP1373570B1 (de) * 2001-03-08 2007-09-12 Invitrogen Corporation Verbessertes in-vitro-synthesesystem
EP1300465A1 (de) * 2001-10-08 2003-04-09 Agrobiogen GmbH Biotechnologie Verfahren und Vorrichtung zur Isolierung von RNS-Proben
EP2163652B2 (de) 2002-01-28 2023-05-31 Life Technologies Corporation Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP3067429A1 (de) * 2002-09-03 2016-09-14 Quanta Biosciences, Inc. Verbesserte verfahren zur cdna-synthese
ES2784011T3 (es) 2002-10-16 2020-09-21 Streck Inc Procedimiento y dispositivo para recoger y preservar las células para su análisis
US7291459B2 (en) * 2002-12-10 2007-11-06 University Of Alabama At Huntsville Nucleic acid detector and method of detecting targets within a sample
WO2004076640A2 (en) * 2003-02-25 2004-09-10 Ambion, Inc. Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same
US20050059054A1 (en) † 2003-07-25 2005-03-17 Richard Conrad Methods and compositions for preparing RNA from a fixed sample
US20050208501A1 (en) 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US8460684B2 (en) 2004-03-19 2013-06-11 Wisconsin Alumni Research Foundation Nuclease inhibitors and methods for their use
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2006051552A2 (en) * 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
DE102005015005A1 (de) * 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
EP2066686A4 (de) 2006-10-06 2010-07-14 Dna Genotek Inc Stabilisierungszusammensetzungen und verfahren zur extraktion von ribonukleinsäure
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
MX2011003480A (es) * 2008-10-06 2011-04-21 Symphogen As Metodo para identificar y seleccionar candidatos de farmaco para productos de farmacos de combinacion.
EP2175020A1 (de) * 2008-10-13 2010-04-14 Roche Diagnostics GmbH Verringerung der RNase-Aktivität in komplexen flüssigen Proben
EP2346993A1 (de) * 2008-10-30 2011-07-27 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Effiziente expression verkürzter menschlicher rnaset2 in e.coli
WO2010071833A1 (en) 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
US11634747B2 (en) * 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
ES2649572T3 (es) 2009-02-18 2018-01-12 Streck Inc. Conservación de ácidos nucleicos fuera de las células
WO2011008553A1 (en) * 2009-06-29 2011-01-20 Life Technologies Corporation Rna sample stabilization in the presence of a transitional metal
WO2011057184A1 (en) 2009-11-09 2011-05-12 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample
CN102243154B (zh) * 2011-04-02 2016-05-11 中山大学附属第一医院 一种周围神经切片染色的方法
WO2012151391A2 (en) 2011-05-04 2012-11-08 Streck, Inc. Inactivated virus compositions and methods of preparing such compositions
MX347611B (es) 2011-06-19 2017-05-04 Abogen Inc Dispositivos, soluciones y metodos para recoleccion de muestras.
WO2013055898A1 (en) * 2011-10-14 2013-04-18 The Trustees Of The University Of Pennsylvania Methods for enhancing the efficacy of microrna mediated interference
US11931740B2 (en) 2012-02-13 2024-03-19 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US11485968B2 (en) 2012-02-13 2022-11-01 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US9101930B2 (en) 2012-02-13 2015-08-11 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
JP6236864B2 (ja) * 2012-06-11 2017-11-29 東ソー株式会社 癌の検出方法及び膵臓特異的リボヌクレアーゼ１を認識する抗体
WO2014047141A1 (en) * 2012-09-19 2014-03-27 Beckman Coulter, Inc. USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS
WO2014066376A1 (en) * 2012-10-25 2014-05-01 Neumodx Molecular, Inc. Method and materials for isolation of nucleic acid materials
WO2015013244A1 (en) 2013-07-24 2015-01-29 Streck, Inc. Compositions and methods for stabilizing circulating tumor cells
CA2939124C (en) 2014-02-07 2023-08-29 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
EP3158085B1 (de) 2014-06-18 2020-12-02 Clear Gene, Inc. Verfahren zur schnellanalyse von biologischen markern
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
ES2963004T3 (es) 2015-09-09 2024-03-22 Drawbridge Health Inc Dispositivos para la recopilación, estabilización y conservación de muestras
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
CA3008273A1 (en) 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
US11506655B2 (en) 2016-07-29 2022-11-22 Streck, Inc. Suspension composition for hematology analysis control
US20230272465A1 (en) * 2020-07-13 2023-08-31 The Board Of Trustees Of The Leland Stanford Junior University Systems and Methods to Enhance RNA Transcription and Uses Thereof
CN114249829B (zh) * 2021-12-20 2022-09-20 南京诺唯赞生物科技股份有限公司 一种RNase抑制剂的单克隆抗体及其制备方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58116498A (ja) 1981-12-28 1983-07-11 Takeda Chem Ind Ltd Ｉｌ‐２をコードする新規伝令ｒｎａの製造法
US5278062A (en) 1987-04-03 1994-01-11 Amgen Proteolytic enzymes
US5151504A (en) * 1989-11-17 1992-09-29 E. R. Squibb & Sons, Inc. Method for purification of monoclonal antibodies
US5470971A (en) 1991-03-11 1995-11-28 The University Of Medicine And Dentistry Of New Jersey Stress-induced proteins, genes coding therefor, transformed cells of organisms, methods and applications
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US6110968A (en) * 1995-12-26 2000-08-29 The Picower Institute For Medical Research Methods for treatment predicated on the presence of advanced glycosylation endproducts in tobacco and its combustion byproducts
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
EP0901369A1 (de) 1996-05-02 1999-03-17 The Procter & Gamble Company Dispergierte tenside-komplexe enthaltende topische zusammensetzungen
JPH11508612A (ja) 1996-05-02 1999-07-27 ザ、プロクター、エンド、ギャンブル、カンパニー 分散した界面活性剤錯体を含む局所用組成物
US5968784A (en) 1997-01-15 1999-10-19 Chugai Pharmaceutical Co., Ltd. Method for analyzing quantitative expression of genes
US5972613A (en) * 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
US6869604B1 (en) * 1998-03-27 2005-03-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinant anti-tumor RNAse
US7264932B2 (en) 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
DE60044977D1 (de) * 1999-09-24 2010-10-28 Ambion Inc Cocktail von nukleaseinhibitoren
US6793019B2 (en) 2002-07-10 2004-09-21 Abb Offshore Systems, Inc. Tapered ramp positive lock latch mechanism
US7021540B2 (en) * 2002-07-30 2006-04-04 Canon Kabushiki Kaisha Storage medium mounting/dismounting mechanism and information processing apparatus
WO2004076640A2 (en) 2003-02-25 2004-09-10 Ambion, Inc. Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same

Also Published As

Publication number Publication date
EP1220944B1 (de) 2010-09-15
EP1220944A1 (de) 2002-07-10
US7445901B2 (en) 2008-11-04
US6664379B1 (en) 2003-12-16
EP1220944A4 (de) 2005-06-22
US20070148174A1 (en) 2007-06-28
ATE481499T1 (de) 2010-10-15
US20050026284A1 (en) 2005-02-03
US7163793B2 (en) 2007-01-16
WO2001021830A1 (en) 2001-03-29

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