ATE430195T1 - Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle - Google Patents

Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle

Info

Publication number
ATE430195T1
ATE430195T1 AT03816282T AT03816282T ATE430195T1 AT E430195 T1 ATE430195 T1 AT E430195T1 AT 03816282 T AT03816282 T AT 03816282T AT 03816282 T AT03816282 T AT 03816282T AT E430195 T1 ATE430195 T1 AT E430195T1
Authority
AT
Austria
Prior art keywords
nucleic acid
compositions
cells
host cell
methods
Prior art date
Application number
AT03816282T
Other languages
English (en)
Inventor
Michael Domanico
Matt Meyers
Kristian Keane
Lisa Braun
Thomas Kolzau
Original Assignee
Qiagen North American Holdings
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings filed Critical Qiagen North American Holdings
Application granted granted Critical
Publication of ATE430195T1 publication Critical patent/ATE430195T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
AT03816282T 2003-03-12 2003-12-16 Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle ATE430195T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/387,646 US20040180445A1 (en) 2003-03-12 2003-03-12 Methods and compositions for purification of nucleic acid from a host cell
PCT/US2003/040175 WO2004081175A2 (en) 2003-03-12 2003-12-16 Methods and compositions for purification of nucleic acid from a host cell

Publications (1)

Publication Number Publication Date
ATE430195T1 true ATE430195T1 (de) 2009-05-15

Family

ID=32961928

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03816282T ATE430195T1 (de) 2003-03-12 2003-12-16 Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle

Country Status (7)

Country Link
US (2) US20040180445A1 (de)
EP (1) EP1601966B1 (de)
CN (1) CN1768265A (de)
AT (1) ATE430195T1 (de)
AU (1) AU2003297244A1 (de)
DE (1) DE60327471D1 (de)
WO (1) WO2004081175A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
AU2004236740A1 (en) * 2003-05-02 2004-11-18 Sigma-Aldrich Co. Solid phase cell lysis and capture platform
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
WO2005054435A2 (en) * 2003-11-26 2005-06-16 Eppendorf Ag Methods and compositions for in vitro amplification of extrachromosomal nucleic acid
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
KR20070103417A (ko) * 2005-01-21 2007-10-23 더 뉴욕 블러드 센터, 인코포레이티드 핵산의 추출 및 확인방법
CN101163802A (zh) * 2005-04-19 2008-04-16 杜邦公司 通过靶基因组的分级分离增加基于核酸的生物体检测的灵敏性
KR100622606B1 (ko) 2005-06-16 2006-09-11 (주)엘피스바이오텍 단일공정에 의한 플라스미드 ｄｎａ 정제용 조성물과 그를이용하여 플라스미드 ｄｎａ를 정제하는 방법
JP2007006799A (ja) * 2005-06-30 2007-01-18 Tokyo Institute Of Technology プラスミドｄｎａの精製方法及びプラスミドｄｎａ精製用キット
US8030034B2 (en) * 2005-12-09 2011-10-04 Promega Corporation Nucleic acid purification with a binding matrix
JP2009528845A (ja) * 2006-03-08 2009-08-13 プロメガ・コーポレーション 低分子量ｒｎａの精製方法
US20080003574A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Kits for RNA extraction
US8012685B2 (en) * 2006-08-01 2011-09-06 Applied Biosystems, Llc Detection of analytes and nucleic acids
DE602007010986D1 (de) * 2006-12-13 2011-01-13 Roche Diagnostics Gmbh Verwendung von acetalen zur isolation von nukleinsäuren
US20080146789A1 (en) * 2007-03-20 2008-06-19 Braman Jeffrey C Methods for the separation of biological molecules using dioxolane
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
WO2009129524A2 (en) * 2008-04-18 2009-10-22 The General Hospital Corporation High-throughput plasmid dna preparation
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
CN107058617A (zh) * 2010-07-29 2017-08-18 霍夫曼-拉罗奇有限公司 通用样品制备
US9725754B2 (en) * 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
WO2012028525A2 (en) * 2010-08-30 2012-03-08 F. Hoffmann-La Roche Ag Method for producing a lipid particle, the lipid particle itself and its use
US8791063B2 (en) 2011-08-25 2014-07-29 Hoffmann-La Roche, Inc. Shortened tetranectin-apolipoprotein A-I fusion protein, a lipid particle containing it, and uses thereof
ES2567091T3 (es) 2012-04-05 2016-04-19 F. Hoffmann-La Roche Ag Compuestos de amina para la preparación selectiva de muestras biológicas
US10072284B2 (en) 2012-06-21 2018-09-11 Monsanto Technology Llc Lysis buffer and methods for extraction of DNA from plant material
CN107254466A (zh) * 2017-07-01 2017-10-17 济凡生物科技（北京）有限公司 一种一步法提取质粒dna的试剂盒和方法
CN113186185B (zh) * 2020-01-14 2023-05-26 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法
CN112626174B (zh) * 2020-12-16 2024-05-07 广州源井生物科技有限公司 一种细胞裂解液及其应用
WO2024006548A1 (en) * 2022-07-01 2024-01-04 Sherlock Biosciences, Inc. Nucleic acid preparation

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1185197A (en) * 1981-04-30 1985-04-09 Akira Furuya Lysozyme-sensitive microorganism
US4652517A (en) * 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
AU609824B2 (en) * 1987-06-15 1991-05-09 Southern Cross Biotech Pty Ltd. Production of proteins in active forms
EP0295859B1 (de) * 1987-06-15 1994-11-17 Southern Cross Biotech Pty.Ltd. Herstellung von Proteinen in aktiver Form
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
GB9020594D0 (en) * 1990-09-21 1990-10-31 Procter & Gamble Cleansing compositions
DE59502334D1 (de) * 1994-01-07 1998-07-02 Qiagen Gmbh Verfahren zum zerkleinern von hochmolekularen strukturen
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
GB2297975B (en) * 1995-01-14 1998-08-05 Procter & Gamble Cleansing compositions
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
GB9607963D0 (en) * 1996-04-17 1996-06-19 Unilever Plc Cleansing composition
EP2290099B1 (de) * 1998-02-02 2012-10-10 QIAGEN North American Holdings, Inc. Verfahren zur Isolierung, Amplifizierung und Charakterisierung von DNS
JP2002531518A (ja) * 1998-12-04 2002-09-24 プロヴァリス・ユーケー・リミテッド インスリン含有医薬組成物
DE10006590B4 (de) * 2000-02-11 2007-10-18 Qiagen North American Holdings, Inc. Verwendung funktionalisierter Membranen bzw. Matrizes zur Aufreinigung von Nukleinsäuren sowie entsprechende Verfahren
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
JP2004504330A (ja) * 2000-07-13 2004-02-12 インヴィトロジェン　コーポレーション 溶解マトリクスを用いてタンパク質およびペプチドの迅速な抽出および単離のための方法および組成物
US6548256B2 (en) * 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
ES2305023T3 (es) * 2000-10-31 2008-11-01 F. Hoffmann-La Roche Ag Metodos para el analisis de componentes no proteinaceos usando una proteasa de una cepa de bacilo.
DE10106199A1 (de) * 2001-02-10 2002-08-22 Eppendorf Ag Verfahren zur Isolierung von Nukleinsäuren aus einer nukleinsäurehaltigen flüssigen Probe
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US6534472B1 (en) * 2001-11-13 2003-03-18 Colgate-Palmolive Company Antibacterial cleaning wipe
WO2004066921A2 (en) * 2003-01-21 2004-08-12 Smithkline Beecham Corporation Erbb4 co-crystal
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor

Also Published As

Publication number Publication date
WO2004081175A2 (en) 2004-09-23
US7863050B2 (en) 2011-01-04
CN1768265A (zh) 2006-05-03
EP1601966B1 (de) 2009-04-29
US20090246859A1 (en) 2009-10-01
AU2003297244A1 (en) 2004-09-30
WO2004081175A3 (en) 2005-02-03
DE60327471D1 (de) 2009-06-10
US20040180445A1 (en) 2004-09-16
EP1601966A4 (de) 2007-05-02
EP1601966A2 (de) 2005-12-07
AU2003297244A8 (en) 2004-09-30

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