NO20063278L - Hurtigfremstilling av nukleinsyrer ved enzymatisk fordoyelse - Google Patents

Hurtigfremstilling av nukleinsyrer ved enzymatisk fordoyelse

Info

Publication number
NO20063278L
NO20063278L NO20063278A NO20063278A NO20063278L NO 20063278 L NO20063278 L NO 20063278L NO 20063278 A NO20063278 A NO 20063278A NO 20063278 A NO20063278 A NO 20063278A NO 20063278 L NO20063278 L NO 20063278L
Authority
NO
Norway
Prior art keywords
nucleic acids
enzymatic digestion
rapid preparation
biological sample
nucleic acid
Prior art date
Application number
NO20063278A
Other languages
English (en)
Inventor
Fuqiang Chen
Original Assignee
Sigma Aldrich Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co filed Critical Sigma Aldrich Co
Publication of NO20063278L publication Critical patent/NO20063278L/no

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Den foreliggende oppfinnelsen tilveiebringer metoder, sammensetninger og sett for isolering og rensing av minst én nukleinsyre direkte fra en biologisk prøve eller for fremstilling av en biologisk prøve for etterfølgende isolering og rensing av minst én nukleinsyre, uten fjerning av den biologiske prøvens cellenæringsmedium eller cellulære væske.
NO20063278A 2003-12-30 2006-07-14 Hurtigfremstilling av nukleinsyrer ved enzymatisk fordoyelse NO20063278L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53362403P 2003-12-30 2003-12-30
PCT/US2004/043980 WO2005068662A1 (en) 2003-12-30 2004-12-30 Rapid preparation of nucleic acids by enzymatic digestion

Publications (1)

Publication Number Publication Date
NO20063278L true NO20063278L (no) 2006-09-29

Family

ID=34794239

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20063278A NO20063278L (no) 2003-12-30 2006-07-14 Hurtigfremstilling av nukleinsyrer ved enzymatisk fordoyelse

Country Status (7)

Country Link
US (1) US20050164260A1 (no)
EP (1) EP1709197A4 (no)
JP (1) JP2007516729A (no)
KR (1) KR20060129340A (no)
IL (1) IL176609A0 (no)
NO (1) NO20063278L (no)
WO (1) WO2005068662A1 (no)

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DE102005057334A1 (de) 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
EP2281826B1 (en) * 2008-04-02 2016-02-17 Rbc Bioscience Corp. Reagents, chaotropic agents, kits and methods for isolating nucleic acids based on magnetic cellulose materials
CN102575220B (zh) 2009-09-03 2015-09-16 贝克顿·迪金森公司 用于直接化学裂解的方法和组合物
US8816064B2 (en) 2010-09-30 2014-08-26 Phynexus, Inc. Purification of nucleic acids
WO2014018195A1 (en) 2012-06-21 2014-01-30 Monsanto Technology Llc Lysis buffer and methods for extraction of dna from plant material
CN103571824B (zh) * 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
TWI555850B (zh) * 2012-08-09 2016-11-01 財團法人工業技術研究院 用於分離核酸的組合物及方法
WO2016183292A1 (en) * 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
AU2017205283A1 (en) * 2016-01-08 2018-07-19 Pathoquest Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in acellular biological fluids
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
CN110249048A (zh) * 2016-09-15 2019-09-17 太阳基因组学公司 用于从样品中的一种或多种类型的微生物的多样性群体中提取核酸分子的通用方法
US10428370B2 (en) 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

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US4843012A (en) * 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
GB8725606D0 (en) * 1987-11-02 1987-12-09 Soc D Etudes Prod Chimique Preparation polynucleotides
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
EP0473575B1 (en) * 1989-05-22 1994-04-13 Genetics Institute, Inc. Improved composition for isolating and purifying nucleic acid and improved method using same
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ プラント ジェネティクス 磁気性粒子を使用する生物学的材料の単離
SE9103215D0 (sv) * 1991-11-04 1991-11-04 Kabi Pharmacia Ab A method and device for dosing a liquid preparation
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5438129A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
US6037465A (en) * 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
US5660984A (en) * 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
DE29601618U1 (de) * 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
DE19821060A1 (de) * 1997-09-23 1999-04-15 Bundesrepublik Deutschland Let Ko-stimulierendes Polypeptid von T-Zellen, monoklonale Antikörper sowie die Herstellung und deren Verwendung
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US6174704B1 (en) * 1998-12-23 2001-01-16 Pierce Chemical Company Method for recovery of proteins prepared by recombinant DNA procedures
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Also Published As

Publication number Publication date
EP1709197A4 (en) 2007-07-04
US20050164260A1 (en) 2005-07-28
JP2007516729A (ja) 2007-06-28
KR20060129340A (ko) 2006-12-15
IL176609A0 (en) 2006-10-31
EP1709197A1 (en) 2006-10-11
WO2005068662A1 (en) 2005-07-28

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