ATE403142T1 - Verfahren zum chrakterisieren von proben von sekundärlicht emittierenden partikeln - Google Patents

Verfahren zum chrakterisieren von proben von sekundärlicht emittierenden partikeln

Info

Publication number
ATE403142T1
ATE403142T1 AT01986914T AT01986914T ATE403142T1 AT E403142 T1 ATE403142 T1 AT E403142T1 AT 01986914 T AT01986914 T AT 01986914T AT 01986914 T AT01986914 T AT 01986914T AT E403142 T1 ATE403142 T1 AT E403142T1
Authority
AT
Austria
Prior art keywords
counting
photons
histogram
molecules
signal
Prior art date
Application number
AT01986914T
Other languages
English (en)
Inventor
Christian Eggeling
Peet Kask
Claus Seidel
Joerg Schaffer
Original Assignee
Evotec Ag
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Ag, Max Planck Gesellschaft filed Critical Evotec Ag
Application granted granted Critical
Publication of ATE403142T1 publication Critical patent/ATE403142T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01986914T 2000-12-21 2001-12-20 Verfahren zum chrakterisieren von proben von sekundärlicht emittierenden partikeln ATE403142T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00128092 2000-12-21
EP00128142 2000-12-21

Publications (1)

Publication Number Publication Date
ATE403142T1 true ATE403142T1 (de) 2008-08-15

Family

ID=26071680

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01986914T ATE403142T1 (de) 2000-12-21 2001-12-20 Verfahren zum chrakterisieren von proben von sekundärlicht emittierenden partikeln

Country Status (6)

Country Link
US (2) US20040099813A1 (de)
EP (1) EP1344043B1 (de)
AT (1) ATE403142T1 (de)
DE (1) DE60135148D1 (de)
DK (1) DK1344043T3 (de)
WO (1) WO2002050516A2 (de)

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Publication number Priority date Publication date Assignee Title
US7810380B2 (en) 2003-03-25 2010-10-12 Tearlab Research, Inc. Systems and methods for collecting tear film and measuring tear film osmolarity
US8020433B2 (en) * 2003-03-25 2011-09-20 Tearlab Research, Inc. Systems and methods for a sample fluid collection device
EP1424548A1 (de) * 2002-09-27 2004-06-02 Evotec OAI AG Partikel-Detektionsverfahren und Mikroskopiereinrichtung zur Partikel-Detektion
EP1617221A4 (de) * 2003-04-23 2006-08-02 Olympus Corp Screening-verfahren für eine zur bindung an einen rezeptor fähige substanz
EP1678495A4 (de) * 2003-10-20 2007-06-27 Univ California Transduktionssysteme im nanomassstab zum nachweis molekularer wechselwirkungen
WO2005107404A2 (en) * 2004-05-03 2005-11-17 The Penn State Research Foundation Methods and systems for nanoparticle enhancement of signals
WO2006014437A2 (en) * 2004-07-07 2006-02-09 The Penn State Research Foundation Methods and systems for nanoparticle enhancement of signals
DE102005008196B4 (de) * 2005-02-22 2019-08-29 Leica Microsystems Cms Gmbh Verfahren zum Erkennen von Dunkelzuständen bei der spektroskopischen oder mikroskopischen Untersuchung von fluoreszenten Proben und Vorrichtung zur spektroskopischen oder mikroskopischen Untersuchung von fluoreszenten Proben
EP2051062B1 (de) * 2007-10-16 2011-02-16 Gwangju Institute of Science and Technology Vorrichtung zur Messung der Fluoreszenzlebenszeit
US8676744B2 (en) * 2007-10-25 2014-03-18 Lawrence Livermore National Security, Llc Physics-based, Bayesian sequential detection method and system for radioactive contraband
DE102008004549B4 (de) * 2008-01-15 2013-04-18 PicoQuant GmbH. Unternehmen für optoelektronische Forschung und Entwicklung Vorrichtung und Verfahren zur simultanen zeitaufgelösten Einzelphotonenregistrierung aus einer Mehrzahl von Detektionskanälen
EP2250530A1 (de) * 2008-02-25 2010-11-17 The University of Melbourne Verfahren zur herstellung einer einzelphotonenquelle
CN110296959B (zh) * 2008-06-10 2022-05-03 嘉睿特热***有限公司 粒子检测
EP2186827A1 (de) 2008-11-14 2010-05-19 HS LifeSciences Ltd. Ersatzmarkergerichtetes cDNA-Klonen von selektiv induzierten mRNAs
DE102010005723A1 (de) * 2010-01-26 2011-07-28 WITec Wissenschaftliche Instrumente und Technologie GmbH, 89081 Raman-Vorrichtung, insbesondere Raman-Mikroskop mit einer Vorrichtung zur Detektion eines Raman-Signals einer Probe
DE102010047237B4 (de) * 2010-08-13 2021-07-01 Leica Microsystems Cms Gmbh Verfahren zum Trennen von Detektionssignalen im Strahlengang einer optischen Einrichtung
WO2012103423A2 (en) * 2011-01-28 2012-08-02 Cornell University Computer-implemented platform for automated fluorescence imaging and kinetic analysis
DE102011052686B4 (de) * 2011-08-12 2013-09-05 Leica Microsystems Cms Gmbh Einrichtung und Verfahren zum Verteilen von Beleuchtungslicht und Detektionslicht in einem Mikroskop in Abhängigkeit des jeweiligen Polarisierungszustands und Mikroskop mit einer solchen Einrichtung
US8982338B2 (en) * 2012-05-31 2015-03-17 Thermo Scientific Portable Analytical Instruments Inc. Sample analysis
EP3699577B1 (de) * 2012-08-20 2023-11-08 Illumina, Inc. System für auf fluoreszenzlebensdauer basierende sequenzierung
WO2014080932A1 (ja) * 2012-11-26 2014-05-30 国立大学法人大阪大学 光学顕微鏡、および、光学顕微鏡のオートフォーカス装置
RU2530468C1 (ru) * 2013-05-17 2014-10-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Нижегородский государственный технический университет им. Р.Е. Алексеева" (НГТУ) Способ измерения параметров однофотонных источников излучения инфракрасного диапазона
CN104165852B (zh) * 2014-08-25 2016-09-28 广西师范大学 一种简单快速测定氟离子的共振瑞利散射能量转移光谱法
AU2015320685B2 (en) 2014-09-23 2021-01-28 Tearlab Research, Inc. Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest
CN111122540A (zh) * 2019-12-25 2020-05-08 桂林电子科技大学 基于时间相关单光子探测技术的多功能光纤探针***
GB2609456A (en) * 2021-08-02 2023-02-08 Advanced Instr Ltd An apparatus and method for calculating a number of photons associated with bound fluorescent molecules

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IL49776A (en) * 1976-06-13 1979-05-31 Elscint Ltd Method and appararus for differentiating of normal and abnormal mammalian cells
US5380663A (en) * 1984-12-24 1995-01-10 Caribbean Microparticles Corporation Automated system for performance analysis and fluorescence quantitation of samples
US5504337A (en) * 1990-10-10 1996-04-02 Joseph R. Lakowicz Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry
US5323008A (en) * 1992-03-23 1994-06-21 Diatron Corporation Fluorometer detection system
US6137584A (en) * 1996-11-27 2000-10-24 Max-Planck-Gesellschaft Zur Method and device for determining predetermined properties of target particles of a sample medium
US6049380A (en) * 1997-11-12 2000-04-11 Regents Of The University Of California Single molecule identification using selected fluorescence characteristics

Also Published As

Publication number Publication date
WO2002050516A3 (en) 2002-09-19
WO2002050516A8 (en) 2002-07-25
DE60135148D1 (de) 2008-09-11
US20070085025A1 (en) 2007-04-19
EP1344043B1 (de) 2008-07-30
DK1344043T3 (da) 2008-11-24
EP1344043A2 (de) 2003-09-17
US20040099813A1 (en) 2004-05-27
WO2002050516A2 (en) 2002-06-27

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