DE60001731D1 - Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen - Google Patents

Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen

Info

Publication number
DE60001731D1
DE60001731D1 DE60001731T DE60001731T DE60001731D1 DE 60001731 D1 DE60001731 D1 DE 60001731D1 DE 60001731 T DE60001731 T DE 60001731T DE 60001731 T DE60001731 T DE 60001731T DE 60001731 D1 DE60001731 D1 DE 60001731D1
Authority
DE
Germany
Prior art keywords
particles
numbers
photon counts
fluorescent molecules
photon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60001731T
Other languages
English (en)
Other versions
DE60001731T2 (de
Inventor
Kauko Palo
Pete Kask
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Evotec OAI AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP99112104A external-priority patent/EP1063502A1/de
Priority claimed from US09/338,439 external-priority patent/US6376843B1/en
Application filed by Evotec OAI AG filed Critical Evotec OAI AG
Publication of DE60001731D1 publication Critical patent/DE60001731D1/de
Application granted granted Critical
Publication of DE60001731T2 publication Critical patent/DE60001731T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/457—Correlation spectrometry, e.g. of the intensity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J2003/2866—Markers; Calibrating of scan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)
  • Spectrometry And Color Measurement (AREA)
DE60001731T 1999-04-29 2000-04-29 Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen Expired - Lifetime DE60001731T2 (de)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US13165799P 1999-04-29 1999-04-29
US131657P 1999-04-29
US338439 1999-06-23
EP99112104A EP1063502A1 (de) 1999-06-23 1999-06-23 Verfahren zur Charakterisierung fluoreszierender Moleküle oder anderer Teilchen unter Verwendung von erzeugenden Funktionen
EP99112104 1999-06-23
US09/338,439 US6376843B1 (en) 1999-06-23 1999-06-23 Method of characterizing fluorescent molecules or other particles using generating functions
EP99125745 1999-12-23
EP99125745 1999-12-23
US18156400P 2000-02-10 2000-02-10
US181564P 2000-02-10
PCT/EP2000/003890 WO2000066985A1 (en) 1999-04-29 2000-04-29 A method of characterizing fluorescent molecules or other particles using generating functions

Publications (2)

Publication Number Publication Date
DE60001731D1 true DE60001731D1 (de) 2003-04-24
DE60001731T2 DE60001731T2 (de) 2004-02-05

Family

ID=56290015

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60001731T Expired - Lifetime DE60001731T2 (de) 1999-04-29 2000-04-29 Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen

Country Status (7)

Country Link
EP (1) EP1175602B1 (de)
JP (1) JP3559525B2 (de)
AT (1) ATE235045T1 (de)
AU (1) AU4559600A (de)
DE (1) DE60001731T2 (de)
DK (1) DK1175602T3 (de)
WO (1) WO2000066985A1 (de)

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US20090172827A1 (en) 2005-05-27 2009-07-02 Johannes Pohlner Kcnn3 as diagnostic and therapeutic target for neurodegenerative diseases
JP4929277B2 (ja) 2005-05-30 2012-05-09 武田薬品工業株式会社 神経変性疾患に対する診断上及び治療上の標的ｐｒｋｘタンパク質
US20090133135A1 (en) 2005-06-01 2009-05-21 Evotec Neurosciences Gmbh Diagnostic and Therapeutic Target SLC39A11 Proteins for Neurodegenerative Diseases
ATE495269T1 (de) 2005-06-16 2011-01-15 Evotec Neurosciences Gmbh Adarb2 proteine als diagnostische und therapeutische targets für neurodegenerative erkrankungen
JP2009278866A (ja) * 2006-09-04 2009-12-03 Olympus Corp Ｄｎａに於けるｄｎａ結合性タンパク質の結合部位の検出方法
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen
US9157804B2 (en) 2009-01-21 2015-10-13 Rare Light, Inc. Correlation interferometric methods, devices and systems for low cost and rugged spectroscopy
CN102869982B (zh) * 2010-03-01 2014-04-30 奥林巴斯株式会社 光学分析装置、光学分析方法
WO2012014778A1 (ja) 2010-07-26 2012-02-02 オリンパス株式会社 発光プローブを用いて溶液中の希薄粒子を検出する方法
WO2012032955A1 (ja) 2010-09-10 2012-03-15 オリンパス株式会社 複数の波長帯域の光計測を用いた光分析方法
CN103097878B (zh) 2010-09-10 2015-07-22 奥林巴斯株式会社 使用单个发光颗粒的光强度的光学分析方法
JP5737704B2 (ja) * 2010-09-27 2015-06-17 株式会社ライフテック 蛍光一粒子検出方法および検出システム
EP2620763A4 (de) 2010-10-19 2015-05-27 Olympus Corp Optische analyse zum beobachten von polarisationsdaten einzelner lichtemittierender teilchen, verfahren für optische analysen und computerprogramm für optische analysen
EP2631631B1 (de) 2010-11-25 2016-01-20 Olympus Corporation Photometrische analysevorrichtung und photometrisches analyseverfahren unter verwendung von wellenlängeneigenschaften eines aus einem einzigen leuchtenden teilchen emittierten lichts
JP5856983B2 (ja) 2011-01-20 2016-02-10 オリンパス株式会社 単一発光粒子からの光の検出を用いた光分析方法及び光分析装置
JP5856984B2 (ja) 2011-01-26 2016-02-10 オリンパス株式会社 核酸分子の多型識別方法
EP2669376B1 (de) 2011-01-26 2017-08-16 Olympus Corporation Verfahren zur identifizierung von polymorphismen von nukleinsäuremolekülen
CN103460026B (zh) 2011-03-29 2015-06-10 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
WO2012141019A1 (ja) 2011-04-13 2012-10-18 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
EP2700935A4 (de) 2011-04-18 2014-10-22 Olympus Corp Quantitatives bestimmungsverfahren für zielpartikel, photometrische analysevorrichtung und computerprogramm für photometrische analysen
JP6013335B2 (ja) 2011-08-11 2016-10-25 オリンパス株式会社 標的粒子の計数方法
JP6013337B2 (ja) 2011-08-15 2016-10-25 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
JP6013338B2 (ja) 2011-08-26 2016-10-25 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
WO2013031309A1 (ja) 2011-08-26 2013-03-07 オリンパス株式会社 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム
CN103765194B (zh) 2011-08-30 2016-02-17 奥林巴斯株式会社 目标粒子的检测方法
CN103765197B (zh) 2011-08-30 2016-03-02 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
WO2013069504A1 (ja) 2011-11-10 2013-05-16 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
DE102012100098B4 (de) * 2012-01-06 2021-09-16 Becker & Hickl Gmbh Verfahren zur Aufzeichnung von zeitlichen Änderungen der Zeitfunktion eines optischen Signals mit räumlicher Auflösung entlang einer Linie im Raum
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CN105593667A (zh) 2013-10-07 2016-05-18 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
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Also Published As

Publication number Publication date
EP1175602B1 (de) 2003-03-19
DK1175602T3 (da) 2003-05-26
EP1175602A1 (de) 2002-01-30
WO2000066985A9 (en) 2002-02-14
JP3559525B2 (ja) 2004-09-02
ATE235045T1 (de) 2003-04-15
JP2002543414A (ja) 2002-12-17
WO2000066985A1 (en) 2000-11-09
AU4559600A (en) 2000-11-17
DE60001731T2 (de) 2004-02-05

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Legal Events

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8327 Change in the person/name/address of the patent owner

Owner name: OLYMPUS CORPORATION, TOKIO/TOKYO, JP

8328 Change in the person/name/address of the agent

Representative=s name: PATENTANWAELTE VON KREISLER, SELTING, WERNER ET COL

8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: PATENTANWAELTE SCHAEFER EMMEL HAUSFELD, 22043 HAMB