ATE360065T1 - Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon - Google Patents
Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davonInfo
- Publication number
- ATE360065T1 ATE360065T1 AT02736176T AT02736176T ATE360065T1 AT E360065 T1 ATE360065 T1 AT E360065T1 AT 02736176 T AT02736176 T AT 02736176T AT 02736176 T AT02736176 T AT 02736176T AT E360065 T1 ATE360065 T1 AT E360065T1
- Authority
- AT
- Austria
- Prior art keywords
- protein synthesis
- dsdna
- fragment
- cell
- pcr
- Prior art date
Links
- 230000014616 translation Effects 0.000 title abstract 6
- 238000001243 protein synthesis Methods 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 10
- 102000053602 DNA Human genes 0.000 abstract 7
- 239000012634 fragment Substances 0.000 abstract 6
- 230000000692 anti-sense effect Effects 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001201356A JP4768155B2 (ja) | 2001-07-02 | 2001-07-02 | 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360065T1 true ATE360065T1 (de) | 2007-05-15 |
Family
ID=19038333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02736176T ATE360065T1 (de) | 2001-07-02 | 2002-06-24 | Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7195895B2 (de) |
| EP (1) | EP1411131B1 (de) |
| JP (1) | JP4768155B2 (de) |
| AT (1) | ATE360065T1 (de) |
| CA (1) | CA2452396A1 (de) |
| DE (1) | DE60219632T2 (de) |
| WO (1) | WO2003004703A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4768155B2 (ja) * | 2001-07-02 | 2011-09-07 | 独立行政法人理化学研究所 | 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法 |
| JP4441170B2 (ja) * | 2002-11-28 | 2010-03-31 | 独立行政法人理化学研究所 | Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法 |
| TW200526786A (en) * | 2003-02-10 | 2005-08-16 | Tatsuya Sawazaki | Automatic process for protein synthesis and apparatus therefor |
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| JP4590249B2 (ja) * | 2004-11-17 | 2010-12-01 | 独立行政法人理化学研究所 | 糖タンパク質合成用の無細胞タンパク質合成システム |
| JP4868731B2 (ja) | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | 哺乳動物培養細胞由来の無細胞タンパク質合成システム |
| JP4787488B2 (ja) * | 2004-11-19 | 2011-10-05 | 独立行政法人理化学研究所 | 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液 |
| US20070117179A1 (en) * | 2005-09-27 | 2007-05-24 | Invitrogen Corporation | In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles |
| WO2008106660A2 (en) * | 2007-03-01 | 2008-09-04 | Invitrogen Corporation | Isolated phospholipid-protein particles |
| CN101849022A (zh) * | 2007-10-22 | 2010-09-29 | 莫诺匡特私人有限公司 | Dna扩增方法 |
| JP5704677B2 (ja) * | 2007-11-05 | 2015-04-22 | 独立行政法人理化学研究所 | 膜タンパク質の製造方法 |
| KR20130023091A (ko) * | 2011-08-26 | 2013-03-07 | (주)바이오니아 | 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법 |
| US20160333397A1 (en) * | 2014-01-27 | 2016-11-17 | Hitachi, Ltd. | Method and device for analyzing reaction liquid after nucleic acid amplification reaction, and device for processing reaction liquid after nucleic acid amplification reaction |
| US11618777B2 (en) | 2015-07-31 | 2023-04-04 | Shigeyuki Yokoyama | Method of manufacturing membrane protein and utilization thereof |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0491790A (ja) | 1990-08-02 | 1992-03-25 | Shionogi & Co Ltd | ２段階ｐｃｒ法 |
| AU1322692A (en) * | 1990-11-05 | 1992-05-26 | United States Of America, As Represented By The Secretary Of The Army, The | Method for the in vitro production of protein from a dna sequence without cloning |
| WO1992011390A1 (en) * | 1990-12-17 | 1992-07-09 | Idexx Laboratories, Inc. | Nucleic acid sequence detection by triple helix formation |
| JPH09234074A (ja) * | 1996-03-04 | 1997-09-09 | Sumitomo Electric Ind Ltd | アダプター二本鎖ｄｎａ及びそれを用いたｄｎａ増幅方法 |
| WO1997046696A1 (en) * | 1996-06-07 | 1997-12-11 | Genentech, Inc. | Methods for in vitro protein synthesis |
| US6136568A (en) * | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
| US6607878B2 (en) * | 1997-10-06 | 2003-08-19 | Stratagene | Collections of uniquely tagged molecules |
| JP2001520054A (ja) | 1997-10-23 | 2001-10-30 | エグザクト サイエンシーズ コーポレイション | Ｐｃｒを用いる分子診断におけるコンタミネーションを検出するための方法 |
| WO1999057992A1 (en) * | 1998-05-14 | 1999-11-18 | Clontech Laboratories, Inc. | Compositions and methods for protein purification based on a metal ion affinity site |
| EP1163359A4 (de) * | 1999-03-24 | 2005-01-26 | Gene Therapy Systems Inc | Verfahren zur erzeugung von transkriptionell aktiven dns-fragmenten |
| US6303337B1 (en) * | 1999-08-25 | 2001-10-16 | Amber Gen. Inc. | N-terminal and C-terminal markers in nascent proteins |
| US7981617B2 (en) * | 2000-08-30 | 2011-07-19 | Cellfree Sciences Co., Ltd. | Transcription template for cell-free protein synthesis and method using the same |
| JP4768155B2 (ja) * | 2001-07-02 | 2011-09-07 | 独立行政法人理化学研究所 | 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法 |
| DE10158904A1 (de) * | 2001-11-30 | 2003-06-12 | Roche Diagnostics Gmbh | Verfahren zur Herstellung von linearen DNA Fragmenten für die in vitro Expression von Proteinen |
| DE60305394T2 (de) | 2002-08-30 | 2007-02-15 | Eastman Kodak Company | Tintenstrahltintenzusammensetzung und druckverfahren |
-
2001
- 2001-07-02 JP JP2001201356A patent/JP4768155B2/ja not_active Expired - Fee Related
-
2002
- 2002-06-24 CA CA002452396A patent/CA2452396A1/en not_active Abandoned
- 2002-06-24 EP EP02736176A patent/EP1411131B1/de not_active Expired - Lifetime
- 2002-06-24 DE DE60219632T patent/DE60219632T2/de not_active Expired - Lifetime
- 2002-06-24 AT AT02736176T patent/ATE360065T1/de not_active IP Right Cessation
- 2002-06-24 WO PCT/JP2002/006261 patent/WO2003004703A1/ja active IP Right Grant
-
2003
- 2003-12-31 US US10/748,055 patent/US7195895B2/en not_active Expired - Lifetime
-
2006
- 2006-03-14 US US11/374,114 patent/US7846694B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1411131A1 (de) | 2004-04-21 |
| CA2452396A1 (en) | 2003-01-16 |
| US7195895B2 (en) | 2007-03-27 |
| US20060147990A1 (en) | 2006-07-06 |
| EP1411131B1 (de) | 2007-04-18 |
| EP1411131A4 (de) | 2005-11-30 |
| WO2003004703A1 (en) | 2003-01-16 |
| JP2003009880A (ja) | 2003-01-14 |
| US20040214292A1 (en) | 2004-10-28 |
| US7846694B2 (en) | 2010-12-07 |
| DE60219632D1 (de) | 2007-05-31 |
| DE60219632T2 (de) | 2008-01-17 |
| JP4768155B2 (ja) | 2011-09-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |