ATE400650T1 - Verfahren zur herstellung von nukleinsäuremolekülen - Google Patents
Verfahren zur herstellung von nukleinsäuremolekülenInfo
- Publication number
- ATE400650T1 ATE400650T1 AT02023385T AT02023385T ATE400650T1 AT E400650 T1 ATE400650 T1 AT E400650T1 AT 02023385 T AT02023385 T AT 02023385T AT 02023385 T AT02023385 T AT 02023385T AT E400650 T1 ATE400650 T1 AT E400650T1
- Authority
- AT
- Austria
- Prior art keywords
- oligonucleotide
- recognition site
- stranded
- restriction enzyme
- ligation product
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP02023385A EP1411122B1 (de) | 2002-10-18 | 2002-10-18 | Verfahren zur Herstellung von Nukleinsäuremolekülen |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE400650T1 true ATE400650T1 (de) | 2008-07-15 |
Family
ID=32039153
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02023385T ATE400650T1 (de) | 2002-10-18 | 2002-10-18 | Verfahren zur herstellung von nukleinsäuremolekülen |
Country Status (9)
Country | Link |
---|---|
US (1) | US7695906B2 (de) |
EP (1) | EP1411122B1 (de) |
AT (1) | ATE400650T1 (de) |
AU (1) | AU2003278103A1 (de) |
CA (1) | CA2502431C (de) |
DE (1) | DE60227525D1 (de) |
DK (1) | DK1411122T3 (de) |
ES (1) | ES2310197T3 (de) |
WO (1) | WO2004035781A1 (de) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
US8137906B2 (en) | 1999-06-07 | 2012-03-20 | Sloning Biotechnology Gmbh | Method for the synthesis of DNA fragments |
DK1314783T3 (da) | 2001-11-22 | 2009-03-16 | Sloning Biotechnology Gmbh | Nukleinsyrelinkere og deres anvendelse i gensyntese |
AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
ATE483020T1 (de) | 2004-01-23 | 2010-10-15 | Sloning Biotechnology Gmbh | Enzymatische herstellung von nukleinsäuremolekülen |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
WO2009082488A2 (en) | 2007-12-24 | 2009-07-02 | Bergenbio As | Methods for creating and identifying functional rna interference elements |
US9115352B2 (en) | 2008-03-31 | 2015-08-25 | Sloning Biotechnology Gmbh | Method for the preparation of a nucleic acid library |
CA2747535C (en) | 2008-12-18 | 2020-01-14 | Iti Scotland Limited | Method for assembly of polynucleic acid sequences |
WO2011032181A2 (en) | 2009-09-14 | 2011-03-17 | Dyax Corp. | Libraries of genetic packages comprising novel hc cdr3 designs |
US9187777B2 (en) | 2010-05-28 | 2015-11-17 | Gen9, Inc. | Methods and devices for in situ nucleic acid synthesis |
GB2481425A (en) | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
EP2609201B1 (de) | 2010-08-26 | 2016-03-02 | E. I. du Pont de Nemours and Company | Mutante delta-9-elongasen und ihre verwendung bei der herstellung von mehrfach ungesättigten fettsäuren |
AU2011293180B2 (en) | 2010-08-26 | 2017-03-02 | E. I. Du Pont De Nemours And Company | Recombinant microbial host cells for high eicosapentaenoic acid production |
EP2638157B1 (de) * | 2010-11-12 | 2015-07-22 | Gen9, Inc. | Verfahren und vorrichtungen für nukleinsäuresynthese |
US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
WO2013017950A1 (en) * | 2011-07-29 | 2013-02-07 | Cellectis | High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules |
WO2013032850A2 (en) | 2011-08-26 | 2013-03-07 | Gen9, Inc. | Compositions and methods for high fidelity assembly of nucleic acids |
CN102584955A (zh) * | 2011-12-31 | 2012-07-18 | 北京唯尚立德生物科技有限公司 | 一种转录激活子样效应因子的固相合成方法 |
DE102012101347B4 (de) * | 2012-02-20 | 2014-01-16 | Markus Fuhrmann | Verfahren zur Herstellung einer Nukleinsäure- Bibliothek mit mindestens zwei benachbarten variablen Codon- Tripletts |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
CN111876409A (zh) | 2012-04-24 | 2020-11-03 | Gen9股份有限公司 | 在体外克隆中分选核酸和多重制备物的方法 |
CN113512577A (zh) | 2012-06-25 | 2021-10-19 | Gen9股份有限公司 | 用于核酸组装和高通量测序的方法 |
GB201612011D0 (en) * | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
GB2566986A (en) | 2017-09-29 | 2019-04-03 | Evonetix Ltd | Error detection during hybridisation of target double-stranded nucleic acid |
CN108913736B (zh) * | 2018-07-10 | 2021-08-24 | 中国海洋大学 | 单链寡核苷酸的制备方法 |
US20230075082A1 (en) * | 2021-03-09 | 2023-03-09 | Smobio Technology, Inc. | Method for synthesizing dna |
WO2022221853A1 (en) | 2021-04-13 | 2022-10-20 | Elegen Corp. | Methods and compositions for cell-free cloning |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5273879A (en) * | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
DE4343591A1 (de) * | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
DE19812103A1 (de) | 1998-03-19 | 1999-09-23 | Bernauer Annette | Verfahren zur Synthese von Nucleinsäuremolekülen |
US6333149B1 (en) | 1999-06-04 | 2001-12-25 | Triad Biotechnology, Inc. | NMR-solve method for rapid identification of bi-ligand drug candidates |
DE19925862A1 (de) * | 1999-06-07 | 2000-12-14 | Diavir Gmbh | Verfahren zur Synthese von DNA-Fragmenten |
JP2001127864A (ja) | 1999-10-29 | 2001-05-11 | Suriibii Enterprises Japan:Kk | 携帯電話機用イヤホンマイク及び携帯電話機 |
-
2002
- 2002-10-18 ES ES02023385T patent/ES2310197T3/es not_active Expired - Lifetime
- 2002-10-18 EP EP02023385A patent/EP1411122B1/de not_active Expired - Lifetime
- 2002-10-18 DK DK02023385T patent/DK1411122T3/da active
- 2002-10-18 DE DE60227525T patent/DE60227525D1/de not_active Expired - Lifetime
- 2002-10-18 AT AT02023385T patent/ATE400650T1/de active
-
2003
- 2003-10-17 AU AU2003278103A patent/AU2003278103A1/en not_active Abandoned
- 2003-10-17 CA CA2502431A patent/CA2502431C/en not_active Expired - Lifetime
- 2003-10-17 WO PCT/EP2003/011551 patent/WO2004035781A1/en not_active Application Discontinuation
- 2003-10-17 US US10/531,556 patent/US7695906B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP1411122A1 (de) | 2004-04-21 |
EP1411122B1 (de) | 2008-07-09 |
CA2502431A1 (en) | 2004-04-29 |
DE60227525D1 (de) | 2008-08-21 |
WO2004035781A1 (en) | 2004-04-29 |
US20060194202A1 (en) | 2006-08-31 |
CA2502431C (en) | 2013-04-02 |
US7695906B2 (en) | 2010-04-13 |
AU2003278103A1 (en) | 2004-05-04 |
ES2310197T3 (es) | 2009-01-01 |
DK1411122T3 (da) | 2008-11-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE400650T1 (de) | Verfahren zur herstellung von nukleinsäuremolekülen | |
ATE483020T1 (de) | Enzymatische herstellung von nukleinsäuremolekülen | |
ATE451342T1 (de) | Verfahren zur herstellung von 1-olefinen durch katalytische spaltung von 1-alkoxyalkanen | |
ATE483032T1 (de) | Verfahren zur herstellung von oligonukleotiden | |
DK1836302T3 (da) | Ligeringsbaseret RNA-amplifikation | |
WO2007010251A3 (en) | Preparation of templates for nucleic acid sequencing | |
WO2007065035A3 (en) | Synthesis of error-minimized nucleic acid molecules | |
ATE414767T1 (de) | Nukleinsäure-linker und deren verwendung in der gensynthese | |
SI2258847T1 (sl) | Nadaljnje nove oblike molekul interferenčne RNA | |
DE60124363D1 (de) | Methode zur Herstellung von genormten und/oder subtrahierten cDNA | |
WO2005030984A3 (en) | Method for small volume nucleic acid synthesis | |
ATE335852T1 (de) | Happier mapping | |
WO2005021786A8 (en) | A method of sequencing nucleic acids by ligation of labelled oligonucleotides | |
WO2003102232A3 (de) | Vermehrung von ribonukleinsäuren | |
WO2005054270A3 (en) | Identification and design of sirna molecules | |
ATE431407T1 (de) | Verfahren zur herstellung von dna | |
WO2002083943A3 (de) | Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen | |
DE60219632D1 (de) | Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon | |
ATE358172T1 (de) | Verfahren zur herstellung von olefinen. | |
DE60123334D1 (de) | Methode für die Produktion von Nukleotiden durch Fermentierung | |
EP1333089A4 (de) | Verfahren zur synthese einzelsträngiger nukleinsäure | |
ATE261981T1 (de) | Verfahren zur herstellung von alkylphosphonsäuren | |
ATE318803T1 (de) | Verfahren zur herstellung von laktonen | |
ATE223499T1 (de) | Von kreisförmiger matrize abhängige kettenreaktion | |
ATE538200T2 (de) | Verfahren zur herstellung einer nukleinsäurenbibliothek |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 1411122 Country of ref document: EP |