ATE287948T1 - Plasmide dna reinigung durch peg-fällung und säulen-chromatographie - Google Patents
Plasmide dna reinigung durch peg-fällung und säulen-chromatographieInfo
- Publication number
- ATE287948T1 ATE287948T1 AT97936375T AT97936375T ATE287948T1 AT E287948 T1 ATE287948 T1 AT E287948T1 AT 97936375 T AT97936375 T AT 97936375T AT 97936375 T AT97936375 T AT 97936375T AT E287948 T1 ATE287948 T1 AT E287948T1
- Authority
- AT
- Austria
- Prior art keywords
- plasmid dna
- column chromatography
- dna purification
- peg precipitation
- dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/692,590 US5707812A (en) | 1996-08-06 | 1996-08-06 | Purification of plasmid DNA during column chromatography |
| PCT/US1997/013647 WO1998005767A1 (en) | 1996-08-06 | 1997-08-05 | Purification of plasmid dna by peg-precipitation and column chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE287948T1 true ATE287948T1 (de) | 2005-02-15 |
Family
ID=24781207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97936375T ATE287948T1 (de) | 1996-08-06 | 1997-08-05 | Plasmide dna reinigung durch peg-fällung und säulen-chromatographie |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5707812A (de) |
| EP (1) | EP0920497B1 (de) |
| JP (1) | JP3905132B2 (de) |
| AT (1) | ATE287948T1 (de) |
| CA (1) | CA2262820C (de) |
| DE (1) | DE69732360T2 (de) |
| WO (1) | WO1998005767A1 (de) |
Families Citing this family (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| US6348450B1 (en) | 1997-08-13 | 2002-02-19 | The Uab Research Foundation | Noninvasive genetic immunization, expression products therefrom and uses thereof |
| US6706693B1 (en) | 1997-08-13 | 2004-03-16 | The Uab Research Foundation | Vaccination by topical application of genetic vectors |
| US20030125278A1 (en) * | 1997-08-13 | 2003-07-03 | Tang De-Chu C. | Immunization of animals by topical applications of a salmonella-based vector |
| US6716823B1 (en) | 1997-08-13 | 2004-04-06 | The Uab Research Foundation | Noninvasive genetic immunization, expression products therefrom, and uses thereof |
| US20030045492A1 (en) * | 1997-08-13 | 2003-03-06 | Tang De-Chu C. | Vaccination by topical application of recombinant vectors |
| US6111096A (en) | 1997-10-31 | 2000-08-29 | Bbi Bioseq, Inc. | Nucleic acid isolation and purification |
| US7626017B2 (en) * | 1997-10-31 | 2009-12-01 | Pressure Biosciences, Inc. | Pressure-enhanced extraction and purification |
| US6214586B1 (en) * | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| DE69922740T2 (de) | 1998-05-11 | 2005-12-08 | Tosoh Corp., Shinnanyo | Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie |
| WO1999058664A1 (en) | 1998-05-14 | 1999-11-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| DE19916534A1 (de) * | 1999-04-13 | 2000-10-19 | Gsf Forschungszentrum Umwelt | RNA-Isolierungs-Kit |
| US20040009936A1 (en) * | 1999-05-03 | 2004-01-15 | Tang De-Chu C. | Vaccine and drug delivery by topical application of vectors and vector extracts |
| IL146695A0 (en) | 1999-05-28 | 2002-07-25 | Bio Science Contract Productio | Methods of dna purification and purified dna |
| US20020010145A1 (en) * | 1999-07-12 | 2002-01-24 | Willson Richard C. | Apparatus, methods and compositions for biotechnical separations |
| US6617108B1 (en) | 1999-07-12 | 2003-09-09 | Technology Licensing Co. Llc | Methods and compositions for biotechnical separations using selective precipitation by compaction agents |
| IL147644A0 (en) | 1999-07-23 | 2002-08-14 | Genentech Inc | Method for rnase-and organic solvent-free plasmid dna purification using tangential flow filtration |
| CA2316113C (en) | 1999-08-18 | 2008-04-01 | David E. Pulleyblank | Method for nucleic acid purification using iodine |
| US6464976B1 (en) | 1999-09-07 | 2002-10-15 | Canji, Inc. | Methods and compositions for reducing immune response |
| US20020012990A1 (en) * | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| DE10006591B4 (de) * | 2000-02-11 | 2007-03-29 | Eppendorf Ag | Verfahren zur Aufreinigung von Nukleinsäuren |
| PT102491B (pt) | 2000-07-10 | 2003-02-28 | Inst Superior Tecnico | Processo para producao e purificacao de dna plasmidico |
| US7074333B2 (en) * | 2001-01-19 | 2006-07-11 | Millipore Corporation | Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration |
| DE10113815A1 (de) * | 2001-03-21 | 2002-10-02 | Eppendorf Ag | Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen |
| US6406892B1 (en) | 2001-05-23 | 2002-06-18 | Bio-Rad Laboratories, Inc. | Acetate-free purification of plasmid DNA on hydroxyapatite |
| US20030211970A1 (en) * | 2001-06-01 | 2003-11-13 | Samuel Nochumson | Processing of plasmid-containing fluids |
| KR100502473B1 (ko) * | 2001-11-08 | 2005-07-20 | 동아제약주식회사 | 플라스미드 ｄｎａ의 분리방법 |
| KR100463415B1 (ko) * | 2001-12-12 | 2004-12-23 | 씨제이 주식회사 | 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법 |
| US6833238B2 (en) * | 2002-01-04 | 2004-12-21 | Applera Corporation | Petal-array support for use with microplates |
| US20040016702A1 (en) * | 2002-07-26 | 2004-01-29 | Applera Corporation | Device and method for purification of nucleic acids |
| AU2003267175A1 (en) | 2002-09-13 | 2004-04-30 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
| US20040157244A1 (en) * | 2002-12-23 | 2004-08-12 | Vical Incorporated | Process for purification of plasmid DNA |
| EP2364769B1 (de) | 2003-05-30 | 2016-01-13 | VGXI, Inc. | Vorrichtungen und Verfahren zur Biomaterialherstellung |
| US20050181378A1 (en) * | 2004-02-18 | 2005-08-18 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| US20050196856A1 (en) * | 2004-02-18 | 2005-09-08 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| US20060160122A1 (en) * | 2004-02-18 | 2006-07-20 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| US7504243B2 (en) | 2004-03-19 | 2009-03-17 | The United States Of America As Represented By The Department Of Health And Human Services | Methods for the production of biliverdin |
| US7527929B2 (en) | 2004-07-30 | 2009-05-05 | Agencourt Bioscience Corporation | Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers |
| JP4971997B2 (ja) | 2005-01-31 | 2012-07-11 | メルク・シャープ・エンド・ドーム・コーポレイション | プラスミドｄｎａの精製方法 |
| AU2006212024A1 (en) * | 2005-02-11 | 2006-08-17 | Dynal Biotech Asa | Method for isolating nucleic acids comprising the use of ethylene glycol multimers |
| CN101163494A (zh) * | 2005-02-23 | 2008-04-16 | Uab研究基金会 | 烷基糖苷增强的接种 |
| WO2006113209A1 (en) * | 2005-04-15 | 2006-10-26 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods and compositions for producing an enhanced immune response to a human papillomavirus immunogen |
| US7960105B2 (en) * | 2005-11-29 | 2011-06-14 | National Institutes Of Health | Method of DNA analysis using micro/nanochannel |
| GB2443505B (en) * | 2006-09-26 | 2008-12-31 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2445441B (en) * | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2445442A (en) * | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
| US20080300397A1 (en) * | 2007-05-31 | 2008-12-04 | Ge Healthcare Uk Limited | Modified spin column for simple and rapid plasmid dna extraction |
| WO2009100172A1 (en) | 2008-02-07 | 2009-08-13 | Ge Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| WO2009111336A2 (en) * | 2008-02-29 | 2009-09-11 | Shizhong Chen | Methods of purifying plasmid dna |
| AU2013329110B2 (en) | 2012-10-12 | 2017-05-04 | Sage Science, Inc. | Side-eluting molecular fractionator |
| US11525155B2 (en) | 2013-03-18 | 2022-12-13 | Qiagen Gmbh | Stabilisation of biological samples |
| EP3207163B1 (de) | 2014-10-15 | 2020-05-27 | Sage Science, Inc. | Vorrichtungen, verfahren und systeme zur automatisierten verarbeitung von nukleinsäuren und elektrophoretischen probenvorbereitung |
| US20170239658A1 (en) * | 2014-10-16 | 2017-08-24 | Ezra S. Abrams | Methods and systems for controlling the shear modulus of genomic length dsdna molecules |
| JP7054678B2 (ja) | 2015-11-20 | 2022-04-14 | ワシントン・ユニバーシティ | ゲノムｄｎａ断片の標的化された精製のための調製用電気泳動方法 |
| US11867661B2 (en) | 2017-04-07 | 2024-01-09 | Sage Science, Inc. | Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification |
| EP3969584A1 (de) * | 2019-05-15 | 2022-03-23 | Translate Bio, Inc. | Verfahren zur reinigung von messenger-rna |
| CN115612684A (zh) * | 2022-11-01 | 2023-01-17 | 北京启辰生生物科技有限公司 | Gmp级质粒dna大规模简化生产方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01304886A (ja) * | 1988-05-31 | 1989-12-08 | Sekisui Chem Co Ltd | Ｄｎａの精製方法 |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
-
1996
- 1996-08-06 US US08/692,590 patent/US5707812A/en not_active Expired - Lifetime
-
1997
- 1997-08-05 AT AT97936375T patent/ATE287948T1/de not_active IP Right Cessation
- 1997-08-05 CA CA002262820A patent/CA2262820C/en not_active Expired - Fee Related
- 1997-08-05 EP EP97936375A patent/EP0920497B1/de not_active Expired - Lifetime
- 1997-08-05 DE DE69732360T patent/DE69732360T2/de not_active Expired - Lifetime
- 1997-08-05 WO PCT/US1997/013647 patent/WO1998005767A1/en active IP Right Grant
- 1997-08-05 JP JP50813098A patent/JP3905132B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0920497B1 (de) | 2005-01-26 |
| JP3905132B2 (ja) | 2007-04-18 |
| EP0920497A1 (de) | 1999-06-09 |
| US5707812A (en) | 1998-01-13 |
| JP2000515385A (ja) | 2000-11-21 |
| WO1998005767A1 (en) | 1998-02-12 |
| DE69732360T2 (de) | 2006-04-06 |
| DE69732360D1 (de) | 2005-03-03 |
| CA2262820A1 (en) | 1998-02-12 |
| CA2262820C (en) | 2007-10-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |