AR245534A1 - Conjunto de reactivos (kit) de aplicacion en inmunoensayos para enzimas - Google Patents

Conjunto de reactivos (kit) de aplicacion en inmunoensayos para enzimas

Info

Publication number
AR245534A1
AR245534A1 AR87307799A AR30779987A AR245534A1 AR 245534 A1 AR245534 A1 AR 245534A1 AR 87307799 A AR87307799 A AR 87307799A AR 30779987 A AR30779987 A AR 30779987A AR 245534 A1 AR245534 A1 AR 245534A1
Authority
AR
Argentina
Prior art keywords
substrate
galos
absorbance
marker
wave
Prior art date
Application number
AR87307799A
Other languages
English (en)
Original Assignee
Senoro Diagnostics Partners
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Senoro Diagnostics Partners filed Critical Senoro Diagnostics Partners
Application granted granted Critical
Publication of AR245534A1 publication Critical patent/AR245534A1/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y10S436/818—Human chorionic gonadotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

UN CONJUNTO DE REACTIVOS PARA REALIZAR INMUNOENSAYOS DE ENZIMAS QUE UTILIZAN UNO O MAS PARES DE ENZIMA MARCADORA/SUBSTRATO QUE CUANTIFICA UNA SUSTANCIA EN ENSAYO MIDIENDO LA ABSORBENCIA DE UN PRODUCTO COLOREADO FORMADO MEDIANTE CONVERSION DEL SUBSTRATO DE UN PAR DE ENZIMA MARCADORA/SUBSTRATO EN DONDE LA ABSORCION DE ENSAYO POR EL PRODUCTO DE LA CONVERSION DE SUBSTRATO MEDIANTE POR LO MENOS UNA ENZIMA MARCADORA EN O CERCA DEL PICO DE LONGITUD DE ONDA PARA LAS MEDICIONES DE ABSORBANCIA PARA QUE EL PAR DE ENZIMAS MARCADORAS/SUBSTRATO EXCEDA EL ALCANCE LINEAL DEL DETECTOR, EN DONDEEL ALCANCE LINEAL DE DICHO ENSAYO SE EXTIENDE SIN REDUCIR SU SENSITIVIDAD MEDIANTE LA MEDICION DE DICHA CONVERSION DE SUBSTRATO EN UNA LONGITUD DE ONDA REDUCIDA EN DONDE LA ABSORBANCIA POR EL PRODUCTO RELEVANTE ES SIGNIFICATIVAMENTE MENOR QUE EN DICHA LONGITUD DE ONDA PICO, Y MEDIANTE EL CALCULO DE LA VERDADERA ABSORBANCIA EN DICHA LONGITUD DE ONDA PICO UTILIZANDO EL RESULTADO DEL ANALISIS DE REGRESION LINEA L DE MEDICION DE ABSORBANCIA OBTENIDA CON LAS CONDICIONES STANDARD DE PRODUCTO EN DICHAS LONGITUDES DE ONDA PICO Y REDUCIDAS DENTRO DEL RANGO LINEAL DEL DETECTOR COMPRENDIENDO POR LO MENOS UN PAR DE ENZIMA MARCADORA- SUBSTRATO SELECCIONADO ENTRE BETA-GALATOSIDASA/NITROFENIL/BETA-D- GALACTOSIDO Y FOSFATASA ALCALINA/MONOFOSFATO DE FENOLFTALEINA, Y OPCIONALMENTE UNA SOLUCION BUFFER.
AR87307799A 1986-06-10 1987-06-08 Conjunto de reactivos (kit) de aplicacion en inmunoensayos para enzimas AR245534A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB868614084A GB8614084D0 (en) 1986-06-10 1986-06-10 Immunoassay

Publications (1)

Publication Number Publication Date
AR245534A1 true AR245534A1 (es) 1994-01-31

Family

ID=10599225

Family Applications (1)

Application Number Title Priority Date Filing Date
AR87307799A AR245534A1 (es) 1986-06-10 1987-06-08 Conjunto de reactivos (kit) de aplicacion en inmunoensayos para enzimas

Country Status (12)

Country Link
US (1) US4914023A (es)
EP (1) EP0249357B1 (es)
JP (1) JPH0718877B2 (es)
AR (1) AR245534A1 (es)
AT (1) ATE93321T1 (es)
AU (1) AU601434B2 (es)
CA (1) CA1284104C (es)
DE (1) DE3787051T2 (es)
ES (1) ES2046203T3 (es)
GB (1) GB8614084D0 (es)
IL (1) IL82790A (es)
ZA (1) ZA874173B (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4954435A (en) * 1987-01-12 1990-09-04 Becton, Dickinson And Company Indirect colorimetric detection of an analyte in a sample using ratio of light signals
IL85581A (en) * 1987-05-21 1992-05-25 Technicon Instr Substrate for beta-galactosidase comprising derivatives of 4-nitrophenyl-beta-d-galactopyranoside and beta-galactosidase immunoassay containing said substrate
GB8801199D0 (en) * 1988-01-20 1988-02-17 Iq Bio Ltd Dual enzyme assay
FR2654836B1 (fr) * 1989-11-17 1994-01-28 Biotrol Sa Laboratoires Appareil d'execution automatique d'un immunodosage en plusieurs etapes successives d'au moins une substance biologique dans une pluralite d'echantillons biologiques, procede et reactif mettant en óoeuvre ledit appareil.
EP0597951B1 (en) * 1991-07-26 1999-03-31 Dade Chemistry Systems Inc. An assay with signal detection in the presence of a suspended solid support
GB9913560D0 (en) * 1999-06-10 1999-08-11 Cortecs Diagnostics Limited Mixing apparatus and method of mixing
DE10223960A1 (de) * 2002-05-29 2003-12-11 Kist Europe Forschungsges Mbh Enzymimmunoassay
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
ES2208121B1 (es) * 2002-11-29 2005-08-16 Consejo Sup. Investig. Cientificas Anticuerpos y antigenos inmovilizados sobre particulas magneticas de silice como biosensores.
CN103890163B (zh) 2011-06-19 2016-09-14 阿博根公司 用于样品采集的装置、溶液和方法
AU2015226811B2 (en) * 2014-03-07 2021-07-15 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
JP2018528763A (ja) 2015-07-31 2018-10-04 リサーチ インスティチュート アット ネイションワイド チルドレンズ ホスピタル バイオフィルムの除去のためのペプチドおよび抗体
WO2017066719A2 (en) * 2015-10-14 2017-04-20 Research Institute At Nationwide Children's Hospital Hu specific interfering agents
CN114813603B (zh) * 2022-03-09 2023-06-20 南京林业大学 一种无标准品快速表征酚羟基类化合物卤代反应转化率的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3174064D1 (en) * 1980-10-15 1986-04-17 Takeda Chemical Industries Ltd Method for immunochemical assay and kit therefor
DE3377531D1 (en) * 1982-09-29 1988-09-01 Serono Diagnostics Ltd Immunoassay of antigens
US4690890A (en) * 1984-04-04 1987-09-01 Cetus Corporation Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay
US4672028A (en) * 1984-05-23 1987-06-09 Icn Micromedic Systems, Inc. Compositions and method for simultaneous multiple array of analytes using radioisotope chelate labels
US4954435A (en) * 1987-01-12 1990-09-04 Becton, Dickinson And Company Indirect colorimetric detection of an analyte in a sample using ratio of light signals

Also Published As

Publication number Publication date
IL82790A (en) 1991-06-30
AU7405987A (en) 1987-12-17
AU601434B2 (en) 1990-09-13
CA1284104C (en) 1991-05-14
US4914023A (en) 1990-04-03
EP0249357A2 (en) 1987-12-16
ATE93321T1 (de) 1993-09-15
ZA874173B (en) 1987-12-11
EP0249357B1 (en) 1993-08-18
EP0249357A3 (en) 1989-10-04
DE3787051D1 (de) 1993-09-23
ES2046203T3 (es) 1994-02-01
JPH0718877B2 (ja) 1995-03-06
JPS6366463A (ja) 1988-03-25
IL82790A0 (en) 1987-12-20
DE3787051T2 (de) 1994-03-03
GB8614084D0 (en) 1986-07-16

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