ES2068860T3 - Potenciacion de señal en un ensayo para una enzima. - Google Patents
Potenciacion de señal en un ensayo para una enzima.Info
- Publication number
- ES2068860T3 ES2068860T3 ES89114719T ES89114719T ES2068860T3 ES 2068860 T3 ES2068860 T3 ES 2068860T3 ES 89114719 T ES89114719 T ES 89114719T ES 89114719 T ES89114719 T ES 89114719T ES 2068860 T3 ES2068860 T3 ES 2068860T3
- Authority
- ES
- Spain
- Prior art keywords
- enzyme
- signal
- liquid
- unknown
- modulator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
EL METODO PARA EL TRATAMIENTO DE UNA ENZIMA DESCONOCIDA QUE SE SUPONE QUE ESTA PRESENTE EN UN LIQUIDO INCLUYE LA AMPLIFICACION DE LA SEÑAL MEDIANTE EL USO DE UNA SEGUNDA ENZIMA Y UN MODULADOR BLOQUEADOR PARA LA SEGUNDA ENZIMA. LA ENZIMA DESCONOCIDA DENTRO DEL LIQUIDO SEPARA UN GRUPO DE BLOQUEO DEL MODULADOR BLOQUEADO. EL MODULADOR RESULTANTE ACTIVA O INHIBE LA SEGUNDA ENZIMA LA CUAL CATALIZA UNA REACCION INDICADORA EN LA CUAL UN SUBSTRATO ES CONVERTIDO EN UN PRODUCTO. LA PRESENCIA O AUSENCIA DE LA ENZIMA DESCONOCIDA EN EL LIQUIDO ES INDICADA POR UNA SEÑAL, TAL COMO UN CAMBIO DE COLOR O UNA PREPARACION DE CAMBIO DE COLOR, ASOCIADA CON LA REACCION INDICADORA. LA CONCENTRACION DE LA ENZIMA EN LA MUESTRA PUEDE SER DETERMINADA MEDIANTE LA MEDIACION DE LA SEÑAL. EL INVENTO INCLUYE UN KIT DE MATERIALES UTILES PARA LA REALIZACION DEL METODO DEL INVENTO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/230,933 US4962024A (en) | 1988-08-11 | 1988-08-11 | Signal enhancement in assay for an enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2068860T3 true ES2068860T3 (es) | 1995-05-01 |
Family
ID=22867123
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89114719T Expired - Lifetime ES2068860T3 (es) | 1988-08-11 | 1989-08-09 | Potenciacion de señal en un ensayo para una enzima. |
Country Status (12)
Country | Link |
---|---|
US (1) | US4962024A (es) |
EP (1) | EP0354548B1 (es) |
JP (1) | JPH02113899A (es) |
AT (1) | ATE119205T1 (es) |
AU (1) | AU621646B2 (es) |
DE (1) | DE68921385T2 (es) |
DK (1) | DK392489A (es) |
ES (1) | ES2068860T3 (es) |
FI (1) | FI95485C (es) |
GR (1) | GR3015261T3 (es) |
MY (1) | MY104160A (es) |
TW (1) | TW204371B (es) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5279937A (en) * | 1992-04-30 | 1994-01-18 | Detechnology Canada | Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays |
DE4217474A1 (de) * | 1992-05-27 | 1993-12-02 | Boehringer Mannheim Gmbh | Verfahren und Mittel zur Bestimmung eines Analyts |
JP3334558B2 (ja) * | 1997-04-23 | 2002-10-15 | 富士レビオ株式会社 | 酵素免疫測定方法及び試験片 |
AU730364B2 (en) * | 1997-09-23 | 2001-03-08 | Bristol-Myers Squibb Company | Selective cPLA2 inhibitors |
DE10135027A1 (de) * | 2001-07-18 | 2003-02-06 | Solvay Pharm Gmbh | Verwendung Trifluoracetylalkyl-substituierter Phenyl-, Phenol- und Benzoylderivate in der Behandlung und/oder Prophylaxe von Obestias und deren Begleit- und/oder Folgeerkrankungen |
WO2003064679A2 (en) * | 2002-01-30 | 2003-08-07 | Id Biomedical Corporation | Methods for detecting vancomycin-resistant microorganisms and compositions therefor |
US20050191704A1 (en) * | 2004-03-01 | 2005-09-01 | Kimberly-Clark Worldwide, Inc. | Assay devices utilizing chemichromic dyes |
US7906276B2 (en) * | 2004-06-30 | 2011-03-15 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
US7094528B2 (en) * | 2004-06-30 | 2006-08-22 | Kimberly-Clark Worldwide, Inc. | Magnetic enzyme detection techniques |
US7402423B2 (en) * | 2004-08-13 | 2008-07-22 | Biomed Solutions, Llc | Apparatus for the detection of pepsin |
US20080057528A1 (en) * | 2006-08-30 | 2008-03-06 | Kimberly-Clark Worldwide, Inc. | Detection of hydrogen peroxide released by enzyme-catalyzed oxidation of an analyte |
EP4183773A1 (en) * | 2010-05-21 | 2023-05-24 | Siemens Healthcare Diagnostics Inc. | Zwitterionic reagents |
CN107827759B (zh) * | 2016-06-29 | 2020-07-31 | 山东转化科技有限公司 | 烷基胺醚衍生表面活性剂及其制备方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3852157A (en) * | 1971-05-14 | 1974-12-03 | Syva Corp | Compounds for enzyme amplification assay |
US4134792A (en) * | 1976-12-06 | 1979-01-16 | Miles Laboratories, Inc. | Specific binding assay with an enzyme modulator as a labeling substance |
CA1190223A (en) * | 1977-11-01 | 1985-07-09 | Anthony C. Richardson | Substrates for enzymes |
US4492751A (en) * | 1978-04-10 | 1985-01-08 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing an enzyme substrate as label |
US4409140A (en) * | 1979-04-23 | 1983-10-11 | Smith Robert E | Substrates and method for determining enzymes |
US4446231A (en) * | 1979-10-03 | 1984-05-01 | Self Colin H | Immunoassay using an amplified cyclic detection system |
DE3274017D1 (en) * | 1981-03-07 | 1986-12-04 | Colin Henry Self | Assay and use |
US4472499A (en) * | 1982-01-22 | 1984-09-18 | American Hoechst Corporation | Reagents for the determination of enzymes |
US4521511A (en) * | 1982-09-22 | 1985-06-04 | Enzyme Technology Company | Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations |
US4463040A (en) * | 1982-12-29 | 1984-07-31 | Polaroid Corporation | Coating-bead stabilization apparatus |
US4810631A (en) * | 1986-05-12 | 1989-03-07 | Becton, Dickinson And Company | Signal enhancement in immunoassay by modulation of chemical catalysis |
US4835099A (en) * | 1986-11-20 | 1989-05-30 | Becton, Dickinson And Company | Signal enhancement in immunoassay by modulation of enzymatic catalysis |
-
1988
- 1988-08-11 US US07/230,933 patent/US4962024A/en not_active Expired - Fee Related
-
1989
- 1989-08-08 AU AU39408/89A patent/AU621646B2/en not_active Ceased
- 1989-08-09 ES ES89114719T patent/ES2068860T3/es not_active Expired - Lifetime
- 1989-08-09 EP EP89114719A patent/EP0354548B1/en not_active Expired - Lifetime
- 1989-08-09 DE DE68921385T patent/DE68921385T2/de not_active Expired - Fee Related
- 1989-08-09 AT AT89114719T patent/ATE119205T1/de not_active IP Right Cessation
- 1989-08-10 FI FI893789A patent/FI95485C/fi not_active IP Right Cessation
- 1989-08-10 MY MYPI89001096A patent/MY104160A/en unknown
- 1989-08-10 DK DK392489A patent/DK392489A/da not_active Application Discontinuation
- 1989-08-11 JP JP1209479A patent/JPH02113899A/ja active Pending
- 1989-09-01 TW TW078106780A patent/TW204371B/zh active
-
1995
- 1995-03-02 GR GR950400384T patent/GR3015261T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
EP0354548A3 (en) | 1991-06-12 |
FI95485B (fi) | 1995-10-31 |
FI893789A0 (fi) | 1989-08-10 |
TW204371B (es) | 1993-04-21 |
FI95485C (fi) | 1996-02-12 |
EP0354548B1 (en) | 1995-03-01 |
AU621646B2 (en) | 1992-03-19 |
DK392489A (da) | 1990-02-12 |
DE68921385D1 (de) | 1995-04-06 |
GR3015261T3 (en) | 1995-06-30 |
DE68921385T2 (de) | 1995-07-27 |
JPH02113899A (ja) | 1990-04-26 |
FI893789A (fi) | 1990-02-12 |
AU3940889A (en) | 1990-02-15 |
ATE119205T1 (de) | 1995-03-15 |
US4962024A (en) | 1990-10-09 |
DK392489D0 (da) | 1989-08-10 |
EP0354548A2 (en) | 1990-02-14 |
MY104160A (en) | 1994-02-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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