ES2130179T3 - Reactivos y metodos de ensayo incluyendo un indicador conteniendo fenazina. - Google Patents

Reactivos y metodos de ensayo incluyendo un indicador conteniendo fenazina.

Info

Publication number
ES2130179T3
ES2130179T3 ES92920269T ES92920269T ES2130179T3 ES 2130179 T3 ES2130179 T3 ES 2130179T3 ES 92920269 T ES92920269 T ES 92920269T ES 92920269 T ES92920269 T ES 92920269T ES 2130179 T3 ES2130179 T3 ES 2130179T3
Authority
ES
Spain
Prior art keywords
analyte
phenazine
semiquinoid
sample
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92920269T
Other languages
English (en)
Inventor
Christopher D Wilsey
Helmut Freitag
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Boehringer Mannheim Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Corp filed Critical Boehringer Mannheim Corp
Application granted granted Critical
Publication of ES2130179T3 publication Critical patent/ES2130179T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LA INVENCION ES RELATIVA A REACTIVO QUE CONTIENE UN COMPUESTO DE FENAZINA Y UNA ENZIMA CON METODOS PARA MEDIR LA CONCENTRACION O DETECTAR LA PRESENCIA DE UN ANALITO EN UNA MUESTRA. EL COMPUESTO QUE CONTIENE FENAZINA DEBE SER DE CLASE SUFICIENTE PARA FORMAR UN SEMIQUINOIDE (EL INDICADOR DE COLOR) POR REACCION INCLUYENDO LA ENZIMA, ANALITO, Y COMPUESTO QUE CONTIENE FENAZINA. SIGNIFICATIVAMENTE, EL COMPUESTO QUE CONTIENE FENAZINA DEBE ESTAR EN CANTIDAD SUFICIENTE PARA CORRELACIONAR LA CONCENTRACION DE SEMIQUINOIDE CON LA CONCENTRACION DE ANALITO DE LA MUESTRA, O PARA DETECTAR LA PRESENCIA DEL ANALITO EN LA MUESTRA. EL REACTIVO PUEDE ADEMAS INCLUIR UN TAMPON Y UN SURFACTANTE. EL REACTIVO PUEDE SER INCORPORADO DENTRO DE UNA PELICULA Y PUEDE ESTAR PROVISTO EN FORMA DE KIT. DICHOS METODOS INCLUYEN MEDIDA (O DETECCION) ESPECTROFOTOMETRICA DEL INDICADOR SEMIQUINOIDE EN LONGITUDES DE ONDA MAYORES DE ALREDEDOR DE580 NANOMETROS, LO CUAL REDUCE LAS INTERFERENCIAS DEBIDAS A LA PRESENCIA DE HEMOGLOBINA, BILIRUBINA Y TURBIDITA. ADICIONALMENTE, ESTOS METODOS DE ENSAYO INCLUYEN PERIODOS DE INCUBACION CORTOS, DE MENOS DE ALREDEDOR DE UN MINUTO PARA MEDIDA DE ABSORCION ESPECTROFOTOMETRICA DE MUESTRAS DE PRUEBA PARA ENSAYOS REALIZADOS SOBRE SOLUCIONES Y MENOS DE ALREDEDOR DE 1,5 MINUTOS PARA MEDIDAS DE REFLECTANCIA O TRANSMITANCIA ESPECTROFOTOMETRICA PARA ENSAYOS REALIZADOS SOBRE PELICULAS.
ES92920269T 1991-09-19 1992-09-18 Reactivos y metodos de ensayo incluyendo un indicador conteniendo fenazina. Expired - Lifetime ES2130179T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US76227891A 1991-09-19 1991-09-19

Publications (1)

Publication Number Publication Date
ES2130179T3 true ES2130179T3 (es) 1999-07-01

Family

ID=25064591

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92920269T Expired - Lifetime ES2130179T3 (es) 1991-09-19 1992-09-18 Reactivos y metodos de ensayo incluyendo un indicador conteniendo fenazina.

Country Status (5)

Country Link
EP (1) EP0606296B1 (es)
JP (1) JP3468517B2 (es)
DE (1) DE69228554T2 (es)
ES (1) ES2130179T3 (es)
WO (1) WO1993006487A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10303265A1 (de) 2003-01-28 2004-07-29 Roche Diagnostics Gmbh Fluorimetrische Bestimmung von Analyten durch ein intramolekulares Quencher-Fluorophor-Konjugat
DE10304448A1 (de) 2003-02-04 2004-08-12 Roche Diagnostics Gmbh Fluorimetrische Bestimmung von Analyten durch Amin-N-Oxide als Redoxindikatoren
US7880061B2 (en) 2003-02-25 2011-02-01 The Curators Of The University Of Missouri Soybean variety SN97-6946
EP1853598B1 (en) 2005-03-01 2012-11-21 Life Technologies Corporation Chemical probe compounds that become fluorescent upon reduction, and methods for their use
US8642847B2 (en) 2007-06-21 2014-02-04 Lee K. French Corn inbreds like FAR601 and hybrids thereof
AU2008308486B2 (en) 2007-10-05 2014-08-07 Corteva Agriscience Llc Methods for transferring molecular substances into plant cells
US8008037B2 (en) 2008-03-27 2011-08-30 Roche Diagnostics Operations, Inc. Matrix composition with alkylphenazine quaternary salt and a nitrosoaniline
EP2362723A1 (en) 2008-11-04 2011-09-07 Dow Agrosciences LLC Omega-9 quality brassica juncea
US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
US10968257B2 (en) 2018-04-03 2021-04-06 The Broad Institute, Inc. Target recognition motifs and uses thereof
US12004476B2 (en) 2022-01-26 2024-06-11 Monsanto Technology Llc. Cotton variety 20R750B3XF
WO2024020360A1 (en) 2022-07-18 2024-01-25 Pairwise Plants Services, Inc. Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc'

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4243539A (en) * 1979-08-06 1981-01-06 Mobil Oil Corporation Antioxidant stabilized lubricant compositions
US4391904A (en) * 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
JPS5818167A (ja) * 1981-07-24 1983-02-02 Fuji Photo Film Co Ltd 分析フイルム及びこれを用いる分析方法
US4849330A (en) * 1984-04-27 1989-07-18 Molecular Devices Corporation Photoresponsive redox detection and discrimination
US4857271A (en) * 1985-02-07 1989-08-15 Eastman Kodak Company Reducible compounds and analytical compositions, elements and methods utilizing same
US4803161A (en) * 1986-01-31 1989-02-07 Eastman Kodak Company Biological and analytical uses of phenalenone and benzphenalenone compounds
US4853186A (en) * 1986-05-30 1989-08-01 Eastman Kodak Company Water-compatible reducible compounds and their use in analytical compositions and methods
US4912035A (en) * 1987-06-11 1990-03-27 Eastman Kodak Company Method for minimizing interference by reductants when detecting cells in biological fluids
US5013669A (en) * 1988-06-01 1991-05-07 Smithkline Diagnostics, Inc. Mass producible biologically active solid phase devices

Also Published As

Publication number Publication date
EP0606296A4 (en) 1997-02-05
WO1993006487A1 (en) 1993-04-01
DE69228554D1 (de) 1999-04-08
EP0606296A1 (en) 1994-07-20
JPH06510905A (ja) 1994-12-08
JP3468517B2 (ja) 2003-11-17
DE69228554T2 (de) 1999-08-19
EP0606296B1 (en) 1999-03-03

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