AR126938A1 - Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento - Google Patents

Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento

Info

Publication number
AR126938A1
AR126938A1 ARP220102358A ARP220102358A AR126938A1 AR 126938 A1 AR126938 A1 AR 126938A1 AR P220102358 A ARP220102358 A AR P220102358A AR P220102358 A ARP220102358 A AR P220102358A AR 126938 A1 AR126938 A1 AR 126938A1
Authority
AR
Argentina
Prior art keywords
gene
max1
plant
glyma
endogenous
Prior art date
Application number
ARP220102358A
Other languages
English (en)
Inventor
Lolita George Mathew
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of AR126938A1 publication Critical patent/AR126938A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Esta invención se refiere a composiciones y métodos para modificar genes de Más Crecimiento Axilar 1 (MAX1) en plantas, opcionalmente, para mejorar la arquitectura de la planta y/o para mejorados rasgos de rendimiento. La invención además se refiere a plantas que tienen mejorada arquitectura de la planta y/o rasgos de rendimiento mejorado producidas usando los métodos y las composiciones de la invención. Reivindicación 1: Una planta o parte de planta de la misma que comprende por lo menos una mutación en un gen endógeno de Más Crecimiento Axilar 1 (MAX1) que codifica un polipéptido de monooxigenasa de citocromo P450 (MAX1). Reivindicación 35: Una célula vegetal que comprende por lo menos una mutación dentro de un gen endógeno de Más Crecimiento Axilar 1 (MAX1), donde la por lo menos una mutación es una sustitución de base, inserción de base, o deleción de base que se introduce usando un sistema de edición que comprende un dominio de unión de ácido nucleico que se une a un sitio diana en el gen endógeno MAX1. Reivindicación 63: Un método para elaborar una planta, que comprende: (a) poner en contacto una población de células vegetales que comprenden un gen endógeno de Más Crecimiento Axilar 1 (MAX1) con una nucleasa unida a un dominio de unión de ácido nucleico (por ej., sistema de edición) que se une a una secuencia (i) que tiene por lo menos 80% de identidad de secuencia con una secuencia de nucleótidos de cualquiera de SEQ ID Nº 69, 70, 93, 94, 115, 116, 140 o 141, (ii) que comprende una región que tiene por lo menos 90% de identidad con cualquiera de SEQ ID Nº 72 - 91, 96 - 113, 118 - 138, o 143 - 164; (iii) que codifica una secuencia de aminoácidos que tiene por lo menos 80% de identidad de secuencia con cualquiera de SEQ ID Nº 71, 95, 117, o 142, y/o (iv) que codifica una región que tiene por lo menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 92, 114, 139 o 165, y/o; (b) seleccionar una célula vegetal de la población de células vegetales en la cual un gen endógeno MAX1 ha sido mutado, a fin de producir una célula vegetal que comprende una mutación en el gen endógeno MAX1; y (c) cultivar la célula vegetal seleccionada hasta lograr una planta. Reivindicación 80: Un ácido nucleico guía que se une a un sitio diana en un gen de Más Crecimiento Axilar 1 (MAX1), donde el sitio diana está en una región del gen MAX1 que tiene por lo menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 72 - 91, 96 - 113, 118 - 138, o 143 - 164, opcionalmente una región del gen MAX1 que tiene por lo menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 77 - 79, 81 - 83, 88, 90, 91, 101 - 103, 105 - 107, 113, 121, 124, 125, 127 - 129, 132 - 138, 148 - 150, 152 - 154, o 160 - 164. Reivindicación 84: Un sistema de edición de genes que comprende una proteína efectora CRISPR-CAS en asociación con un ácido nucleico guía, donde el ácido nucleico guía comprende una secuencia espaciadora que se une a un gen endógeno de Más Crecimiento Axilar 1 (MAX1). Reivindicación 91: Una planta de soja o parte de planta de ella que comprende por lo menos una mutación no natural en por lo menos un gen endógeno de Más Crecimiento Axilar 1 (MAX1) que tiene el número de identificación de gen (gen ID) de Glyma.04g052100 (MAX1a), Glyma.06g052700 (MAX1b), Glyma.14g096900 (MAX1c) y/o Glyma.17g227500 (MAX1d). Reivindicación 92: Un ácido nucleico guía que se une a un ácido nucleico diana en un gen de Más Crecimiento Axilar 1 (MAX1) que tiene el número de identificación de gen (gen ID) de Glyma.04g052100 (MAX1a), Glyma.06g052700 (MAX1b), Glyma.14g096900 (MAX1c) y/o Glyma.17g227500 (MAX1d).
ARP220102358A 2021-09-02 2022-08-31 Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento AR126938A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163240132P 2021-09-02 2021-09-02

Publications (1)

Publication Number Publication Date
AR126938A1 true AR126938A1 (es) 2023-11-29

Family

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Family Applications (1)

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ARP220102358A AR126938A1 (es) 2021-09-02 2022-08-31 Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento

Country Status (4)

Country Link
US (1) US20230151379A1 (es)
AR (1) AR126938A1 (es)
CA (1) CA3230668A1 (es)
WO (1) WO2023034891A1 (es)

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