AR106616A1 - Generación de loci de rasgos complejos en soja y métodos de uso - Google Patents

Generación de loci de rasgos complejos en soja y métodos de uso

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Publication number
AR106616A1
AR106616A1 ARP160103396A ARP160103396A AR106616A1 AR 106616 A1 AR106616 A1 AR 106616A1 AR P160103396 A ARP160103396 A AR P160103396A AR P160103396 A ARP160103396 A AR P160103396A AR 106616 A1 AR106616 A1 AR 106616A1
Authority
AR
Argentina
Prior art keywords
target site
double chain
site
methods
endonuclease
Prior art date
Application number
ARP160103396A
Other languages
English (en)
Inventor
K Young Joshua
Bryan Woodward John
Lu Zhan
J Bin Kinney Anthony
Mark Cigan Andrew
Marco Chaky Julian
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of AR106616A1 publication Critical patent/AR106616A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporciona composiciones y métodos para producir un locus de rasgo complejo en una ventana genómica de una planta de soja que comprende (i) al menos un sitio objetivo transgénico para integración sitio-específica integrado en al menos un sitio objetivo de rotura de cadena doble, (ii) al menos un sitio objetivo de rotura de cadena doble y al menos un transgén, (iii) al menos un sitio objetivo de rotura de cadena doble alterado o (iv) cualquier combinación de (i) - (iii). El sitio objetivo de rotura de la cadena doble puede ser., pero no se limita a, un sitio objetivo para una endonucleasa con dedos de zinc, una endonucleasa transformada por ingeniería genética, una meganucleasa, un TALEN y/o una endonucleasa Cas. La ventana genómica de la planta puede comprender al menos un locus genómico de interés tal como un casete de rasgos, un transgén, un gen mutado, un gen nativo, un gen editado o un sitio objetivo para la integración sitio-específica (SSI).
ARP160103396A 2015-11-06 2016-11-07 Generación de loci de rasgos complejos en soja y métodos de uso AR106616A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562251847P 2015-11-06 2015-11-06

Publications (1)

Publication Number Publication Date
AR106616A1 true AR106616A1 (es) 2018-01-31

Family

ID=57233952

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160103396A AR106616A1 (es) 2015-11-06 2016-11-07 Generación de loci de rasgos complejos en soja y métodos de uso

Country Status (4)

Country Link
US (1) US20180258438A1 (es)
AR (1) AR106616A1 (es)
BR (1) BR112018007796A2 (es)
WO (1) WO2017079026A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10030245B2 (en) 2011-03-23 2018-07-24 E I Du Pont De Nemours And Company Methods for producing a complex transgenic trait locus
CN108064129A (zh) 2014-09-12 2018-05-22 纳幕尔杜邦公司 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法
CN108009401B (zh) * 2017-11-29 2021-11-02 内蒙古大学 一种筛选指纹图谱遗传标记的方法
EP3894550A4 (en) 2018-12-14 2023-01-04 Pioneer Hi-Bred International, Inc. NEW CRISPR-CAS SYSTEMS FOR GENOME EDITING
CA3186862A1 (en) 2020-08-18 2022-02-24 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof

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BR112018007796A2 (pt) 2018-10-30
WO2017079026A1 (en) 2017-05-11
US20180258438A1 (en) 2018-09-13

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