AR066196A1 - Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono - Google Patents

Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono

Info

Publication number
AR066196A1
AR066196A1 ARP080101213A ARP080101213A AR066196A1 AR 066196 A1 AR066196 A1 AR 066196A1 AR P080101213 A ARP080101213 A AR P080101213A AR P080101213 A ARP080101213 A AR P080101213A AR 066196 A1 AR066196 A1 AR 066196A1
Authority
AR
Argentina
Prior art keywords
strain
production
carbon source
propanediol
microorganism
Prior art date
Application number
ARP080101213A
Other languages
English (en)
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of AR066196A1 publication Critical patent/AR066196A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B10/00—Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procedimiento para la preparacion de una cepa del microorganismo para la produccion de 1,2-propanodiol a partir de una fuente de carbono. Este procedimiento comprende: el crecimiento de una cepa inicial bajo presion selectiva en un medio de crecimiento apropiado, dicha cepa bacteriana inicial formada por una atenuacion de la expresion del gen tpiA y una atenuacion de la expresion de al menos un gen que participa en la conversion del metilglioxal en lactato para promover la evolucion en dicha cepa inicial, la seleccion y aislamiento de la cepa evolucionada con un aumento de la tasa de produccion de 1,2-propanodiol, la reconstruccion de un gen tpiA funcional en la cepa evolucionada. También se refiere a la cepa evolucionada obtenida, que además puede estar genéticamente modificada para optimizar la conversion de una fuente de carbono en 1,2-propanodiol sin subproductos y con la mejor produccion posible.
ARP080101213A 2007-03-23 2008-03-25 Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono AR066196A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IB2007001680 2007-03-23

Publications (1)

Publication Number Publication Date
AR066196A1 true AR066196A1 (es) 2009-08-05

Family

ID=38814484

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080101213A AR066196A1 (es) 2007-03-23 2008-03-25 Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono

Country Status (14)

Country Link
US (1) US8298807B2 (es)
EP (1) EP2109681B1 (es)
JP (1) JP5570821B2 (es)
KR (1) KR101528943B1 (es)
CN (1) CN101641446A (es)
AR (1) AR066196A1 (es)
BR (1) BRPI0808972B1 (es)
CA (1) CA2679989C (es)
DK (1) DK2109681T3 (es)
ES (1) ES2525796T3 (es)
IL (1) IL200705A0 (es)
MX (1) MX2009010229A (es)
TW (1) TW200904984A (es)
WO (1) WO2008116852A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
BRPI0823256B8 (pt) * 2008-11-07 2020-05-26 Metabolic Explorer Sa uso de sacarose como substrato para produção fermentativa de 1,2-propanodiol
EP2233562A1 (en) 2009-03-24 2010-09-29 Metabolic Explorer Method for producing high amount of glycolic acid by fermentation
MY187676A (en) 2009-06-04 2021-10-08 Genomatica Inc Microorganisms for the production of 1,4-butanediol and related methods
WO2011012702A1 (en) 2009-07-30 2011-02-03 Metabolic Explorer Mutant glycerol dehydrogenase (glydh) for the production of a biochemical by fermentation
BR112012002153A2 (pt) 2009-07-30 2015-09-15 Metabolic Explorer Sa metilglioxal sintase mutante (mgs) para a produção de material bioquímico por fermentação
WO2011012697A2 (en) 2009-07-30 2011-02-03 Metabolic Explorer Mutant yqhd enzyme for the production of a biochemical by fermentation
EP2532751A1 (en) * 2011-06-10 2012-12-12 Metabolic Explorer Use of inducible promoters in the fermentative production of 1,2-propanediol
EP2721167A1 (en) 2011-06-15 2014-04-23 B.R.A.I.N. Biotechnology Research And Information Network AG New means and methods for producing propanediol
EP3050970B1 (en) 2015-01-28 2019-09-18 Metabolic Explorer Modified microorganism for optimized production of 1,4-butanediol
US10415062B2 (en) 2015-04-07 2019-09-17 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxybutyrate
CN107771214B (zh) 2015-04-07 2022-01-18 代谢探索者公司 用于具有增加的2,4-二羟基丁酸外排物的优化的2,4-二羟基丁酸产生的修饰的微生物
PL3331998T3 (pl) 2015-08-07 2020-12-28 Evonik Operations Gmbh Wytwarzanie przez fermentację l-metioniny zależne od tiokarboksylanu białka
EP3347478B1 (en) 2015-09-10 2022-09-21 Metabolic Explorer New lactaldehyde reductases for the production of 1,2-propanediol
EP3365427B1 (en) 2015-10-23 2020-08-19 Metabolic Explorer Microorganism modified for the assimilation of levulinic acid
MX2018006434A (es) 2015-11-27 2018-09-28 Evonik Degussa Gmbh Metodo para producir l-metionina.
KR102430878B1 (ko) 2016-07-08 2022-08-09 메타볼릭 익스플로러 당 포스포트랜스퍼라제 시스템 (pts)을 코딩하는 유전자를 포함하는 미생물에 의한 관심 분자의 발효적 생산을 위한 방법
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
EP3342873A1 (en) 2016-12-29 2018-07-04 Metabolic Explorer Conversion of methylglyoxal into hydroxyacetone using enzymes and applications thereof
EP3470512A1 (en) 2017-10-10 2019-04-17 Metabolic Explorer Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
KR102245274B1 (ko) 2019-01-28 2021-04-27 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 최소 유전체를 갖는 신규 미생물 및 이의 제조 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087140A (en) 1997-02-19 2000-07-11 Wisconsin Alumni Research Foundation Microbial production of 1,2-propanediol from sugar
ES2356526T3 (es) * 2002-10-04 2011-04-08 E.I. Du Pont De Nemours And Company Procedimiento para la producción biológica de 1,3-propanodiol con elevado rendimiento.
JP2006517796A (ja) 2003-02-18 2006-08-03 メタボリック エクスプローラー 代謝経路の生成または改変を可能とする進化した微生物の生産方法
FR2864967B1 (fr) 2004-01-12 2006-05-19 Metabolic Explorer Sa Microorganisme evolue pour la production de 1,2-propanediol

Also Published As

Publication number Publication date
WO2008116852A1 (en) 2008-10-02
TW200904984A (en) 2009-02-01
BRPI0808972B1 (pt) 2016-11-29
CA2679989C (en) 2015-07-07
CN101641446A (zh) 2010-02-03
EP2109681B1 (en) 2014-10-15
ES2525796T3 (es) 2014-12-30
DK2109681T3 (en) 2014-12-08
US8298807B2 (en) 2012-10-30
CA2679989A1 (en) 2008-10-02
JP2010521958A (ja) 2010-07-01
IL200705A0 (en) 2010-05-17
US20100285547A1 (en) 2010-11-11
BRPI0808972A2 (pt) 2014-08-26
MX2009010229A (es) 2009-10-26
EP2109681A1 (en) 2009-10-21
JP5570821B2 (ja) 2014-08-13
KR101528943B1 (ko) 2015-06-15
KR20100015810A (ko) 2010-02-12

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