AR066196A1 - Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono - Google Patents
Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbonoInfo
- Publication number
- AR066196A1 AR066196A1 ARP080101213A ARP080101213A AR066196A1 AR 066196 A1 AR066196 A1 AR 066196A1 AR P080101213 A ARP080101213 A AR P080101213A AR P080101213 A ARP080101213 A AR P080101213A AR 066196 A1 AR066196 A1 AR 066196A1
- Authority
- AR
- Argentina
- Prior art keywords
- strain
- production
- carbon source
- propanediol
- microorganism
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B10/00—Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Procedimiento para la preparacion de una cepa del microorganismo para la produccion de 1,2-propanodiol a partir de una fuente de carbono. Este procedimiento comprende: el crecimiento de una cepa inicial bajo presion selectiva en un medio de crecimiento apropiado, dicha cepa bacteriana inicial formada por una atenuacion de la expresion del gen tpiA y una atenuacion de la expresion de al menos un gen que participa en la conversion del metilglioxal en lactato para promover la evolucion en dicha cepa inicial, la seleccion y aislamiento de la cepa evolucionada con un aumento de la tasa de produccion de 1,2-propanodiol, la reconstruccion de un gen tpiA funcional en la cepa evolucionada. También se refiere a la cepa evolucionada obtenida, que además puede estar genéticamente modificada para optimizar la conversion de una fuente de carbono en 1,2-propanodiol sin subproductos y con la mejor produccion posible.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IB2007001680 | 2007-03-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR066196A1 true AR066196A1 (es) | 2009-08-05 |
Family
ID=38814484
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP080101213A AR066196A1 (es) | 2007-03-23 | 2008-03-25 | Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono |
Country Status (14)
Country | Link |
---|---|
US (1) | US8298807B2 (es) |
EP (1) | EP2109681B1 (es) |
JP (1) | JP5570821B2 (es) |
KR (1) | KR101528943B1 (es) |
CN (1) | CN101641446A (es) |
AR (1) | AR066196A1 (es) |
BR (1) | BRPI0808972B1 (es) |
CA (1) | CA2679989C (es) |
DK (1) | DK2109681T3 (es) |
ES (1) | ES2525796T3 (es) |
IL (1) | IL200705A0 (es) |
MX (1) | MX2009010229A (es) |
TW (1) | TW200904984A (es) |
WO (1) | WO2008116852A1 (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
BRPI0823256B8 (pt) * | 2008-11-07 | 2020-05-26 | Metabolic Explorer Sa | uso de sacarose como substrato para produção fermentativa de 1,2-propanodiol |
EP2233562A1 (en) | 2009-03-24 | 2010-09-29 | Metabolic Explorer | Method for producing high amount of glycolic acid by fermentation |
MY187676A (en) | 2009-06-04 | 2021-10-08 | Genomatica Inc | Microorganisms for the production of 1,4-butanediol and related methods |
WO2011012702A1 (en) | 2009-07-30 | 2011-02-03 | Metabolic Explorer | Mutant glycerol dehydrogenase (glydh) for the production of a biochemical by fermentation |
BR112012002153A2 (pt) | 2009-07-30 | 2015-09-15 | Metabolic Explorer Sa | metilglioxal sintase mutante (mgs) para a produção de material bioquímico por fermentação |
WO2011012697A2 (en) | 2009-07-30 | 2011-02-03 | Metabolic Explorer | Mutant yqhd enzyme for the production of a biochemical by fermentation |
EP2532751A1 (en) * | 2011-06-10 | 2012-12-12 | Metabolic Explorer | Use of inducible promoters in the fermentative production of 1,2-propanediol |
EP2721167A1 (en) | 2011-06-15 | 2014-04-23 | B.R.A.I.N. Biotechnology Research And Information Network AG | New means and methods for producing propanediol |
EP3050970B1 (en) | 2015-01-28 | 2019-09-18 | Metabolic Explorer | Modified microorganism for optimized production of 1,4-butanediol |
US10415062B2 (en) | 2015-04-07 | 2019-09-17 | Metabolic Explorer | Modified microorganism for the optimized production of 2,4-dihydroxybutyrate |
CN107771214B (zh) | 2015-04-07 | 2022-01-18 | 代谢探索者公司 | 用于具有增加的2,4-二羟基丁酸外排物的优化的2,4-二羟基丁酸产生的修饰的微生物 |
PL3331998T3 (pl) | 2015-08-07 | 2020-12-28 | Evonik Operations Gmbh | Wytwarzanie przez fermentację l-metioniny zależne od tiokarboksylanu białka |
EP3347478B1 (en) | 2015-09-10 | 2022-09-21 | Metabolic Explorer | New lactaldehyde reductases for the production of 1,2-propanediol |
EP3365427B1 (en) | 2015-10-23 | 2020-08-19 | Metabolic Explorer | Microorganism modified for the assimilation of levulinic acid |
MX2018006434A (es) | 2015-11-27 | 2018-09-28 | Evonik Degussa Gmbh | Metodo para producir l-metionina. |
KR102430878B1 (ko) | 2016-07-08 | 2022-08-09 | 메타볼릭 익스플로러 | 당 포스포트랜스퍼라제 시스템 (pts)을 코딩하는 유전자를 포함하는 미생물에 의한 관심 분자의 발효적 생산을 위한 방법 |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
EP3342873A1 (en) | 2016-12-29 | 2018-07-04 | Metabolic Explorer | Conversion of methylglyoxal into hydroxyacetone using enzymes and applications thereof |
EP3470512A1 (en) | 2017-10-10 | 2019-04-17 | Metabolic Explorer | Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate |
KR102245274B1 (ko) | 2019-01-28 | 2021-04-27 | 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 | 최소 유전체를 갖는 신규 미생물 및 이의 제조 방법 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6087140A (en) | 1997-02-19 | 2000-07-11 | Wisconsin Alumni Research Foundation | Microbial production of 1,2-propanediol from sugar |
ES2356526T3 (es) * | 2002-10-04 | 2011-04-08 | E.I. Du Pont De Nemours And Company | Procedimiento para la producción biológica de 1,3-propanodiol con elevado rendimiento. |
JP2006517796A (ja) | 2003-02-18 | 2006-08-03 | メタボリック エクスプローラー | 代謝経路の生成または改変を可能とする進化した微生物の生産方法 |
FR2864967B1 (fr) | 2004-01-12 | 2006-05-19 | Metabolic Explorer Sa | Microorganisme evolue pour la production de 1,2-propanediol |
-
2008
- 2008-03-21 WO PCT/EP2008/053445 patent/WO2008116852A1/en active Application Filing
- 2008-03-21 ES ES08714246.9T patent/ES2525796T3/es active Active
- 2008-03-21 MX MX2009010229A patent/MX2009010229A/es active IP Right Grant
- 2008-03-21 KR KR1020097022123A patent/KR101528943B1/ko active IP Right Grant
- 2008-03-21 CN CN200880009616A patent/CN101641446A/zh active Pending
- 2008-03-21 TW TW097110003A patent/TW200904984A/zh unknown
- 2008-03-21 BR BRPI0808972A patent/BRPI0808972B1/pt not_active IP Right Cessation
- 2008-03-21 JP JP2009554045A patent/JP5570821B2/ja not_active Expired - Fee Related
- 2008-03-21 US US12/532,405 patent/US8298807B2/en active Active
- 2008-03-21 DK DK08714246.9T patent/DK2109681T3/en active
- 2008-03-21 CA CA2679989A patent/CA2679989C/en active Active
- 2008-03-21 EP EP08714246.9A patent/EP2109681B1/en not_active Not-in-force
- 2008-03-25 AR ARP080101213A patent/AR066196A1/es active IP Right Grant
-
2009
- 2009-09-03 IL IL200705A patent/IL200705A0/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
WO2008116852A1 (en) | 2008-10-02 |
TW200904984A (en) | 2009-02-01 |
BRPI0808972B1 (pt) | 2016-11-29 |
CA2679989C (en) | 2015-07-07 |
CN101641446A (zh) | 2010-02-03 |
EP2109681B1 (en) | 2014-10-15 |
ES2525796T3 (es) | 2014-12-30 |
DK2109681T3 (en) | 2014-12-08 |
US8298807B2 (en) | 2012-10-30 |
CA2679989A1 (en) | 2008-10-02 |
JP2010521958A (ja) | 2010-07-01 |
IL200705A0 (en) | 2010-05-17 |
US20100285547A1 (en) | 2010-11-11 |
BRPI0808972A2 (pt) | 2014-08-26 |
MX2009010229A (es) | 2009-10-26 |
EP2109681A1 (en) | 2009-10-21 |
JP5570821B2 (ja) | 2014-08-13 |
KR101528943B1 (ko) | 2015-06-15 |
KR20100015810A (ko) | 2010-02-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AR066196A1 (es) | Procedimiento para la preparacion de un microorganismo para la produccion de 1,2- propaindiol a partir de una fuente de carbono | |
PE20140009A1 (es) | Produccion de alquenos mediante conversion enzimatica combinada de acidos 3-hidroxialcanoicos | |
AR065833A1 (es) | Procedimiento para la produccion biologica de 1,3-propanodiol a partir de glicerina con un alto rendimiento | |
ES2673865T3 (es) | Producción de xilitol a partir de glucosa mediante una cepa recombinante | |
AR086772A1 (es) | Variantes del promotor del gen gap que codifica gliceraldehido-3-fosfato deshidrogenasa | |
PE20141569A1 (es) | Metodo para producir una sustancia diana mediante fermentacion | |
AR074092A1 (es) | Uso de sacarosa como sustrato para la produccion fermentativa de 1,2-propanodiol | |
UA108468C2 (en) | Microorganism that expresses xylose isomerase | |
AR082114A1 (es) | Metodo para la preparacion de 1,3-propanodiol a partir de sacarosa | |
AR075927A1 (es) | Procedimiento para la produccion de glioxilato mediante fermentacion | |
ES2667718T3 (es) | Métodos y composiciones para producir hidrocarburos | |
MX2010002825A (es) | Aceites biologicos y produccion y usos de los mismos. | |
ES2597877T3 (es) | Promotor mejorado y método para la producción de L-lisina mediante el uso del mismo | |
MX2009010224A (es) | Microorganismos y metodos para la produccion de 1,2-propanodiol y acetol. | |
AR102678A1 (es) | Producción de ácidos grasos y derivados | |
CO2018011096A2 (es) | Procedimiento para la producción de ácido láctico | |
BRPI0916147A8 (pt) | Cepa de saccharomyces ap., método para a produção de um produto de fermentação e massa celular, e, uso da cepa de saccharomyces sp. | |
AR082517A1 (es) | Produccion de isomaltooligosacaridos y sus usos | |
WO2012018699A3 (en) | Improved fermentation process for the production of organic acids | |
WO2008005988A3 (en) | Production of stilbenes and derivatives in plant hairy root cultures | |
AR083613A1 (es) | Proceso para la produccion de acido polilactico usando monascus | |
AR100382A1 (es) | Método para producir nailon | |
PH12021550347A1 (en) | MICROORGANISM FOR PRODUCING L-AMINO ACID WITH ENHANCED ACTIVITY OF a-GLUCOSIDASE AND METHOD FOR PRODUCING L-AMINO ACID USING THE SAME | |
AR079764A1 (es) | Aumento de la produccion de metionina mediante la sobreexpresion de succinato deshidrogenasa | |
CU23849A3 (es) | Producción aumentada de etanol por células bacterianas |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant, registration |