ZA200302998B - Ethanol production - Google Patents
Ethanol production Download PDFInfo
- Publication number
- ZA200302998B ZA200302998B ZA200302998A ZA200302998A ZA200302998B ZA 200302998 B ZA200302998 B ZA 200302998B ZA 200302998 A ZA200302998 A ZA 200302998A ZA 200302998 A ZA200302998 A ZA 200302998A ZA 200302998 B ZA200302998 B ZA 200302998B
- Authority
- ZA
- South Africa
- Prior art keywords
- gene
- sequence
- gram
- bacterium
- ethanol
- Prior art date
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 168
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 64
- 238000000034 method Methods 0.000 claims abstract description 54
- 238000000855 fermentation Methods 0.000 claims abstract description 44
- 230000004151 fermentation Effects 0.000 claims abstract description 43
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 40
- 238000002744 homologous recombination Methods 0.000 claims abstract description 22
- 230000006801 homologous recombination Effects 0.000 claims abstract description 22
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 241000193390 Parageobacillus thermoglucosidasius Species 0.000 claims abstract 4
- 241000193749 Bacillus coagulans Species 0.000 claims abstract 2
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract 2
- 241001468175 Geobacillus thermodenitrificans Species 0.000 claims abstract 2
- 241000193761 [Bacillus] caldolyticus Species 0.000 claims abstract 2
- 241000193758 [Bacillus] caldotenax Species 0.000 claims abstract 2
- 241000894006 Bacteria Species 0.000 claims description 52
- 239000013612 plasmid Substances 0.000 claims description 41
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 24
- 238000003780 insertion Methods 0.000 claims description 21
- 230000037431 insertion Effects 0.000 claims description 21
- 229920001184 polypeptide Polymers 0.000 claims description 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 20
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 20
- 101150067366 adh gene Proteins 0.000 claims description 19
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 16
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 16
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims description 15
- 230000010354 integration Effects 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 15
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 13
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- 241000588902 Zymomonas mobilis Species 0.000 claims description 8
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- 230000000694 effects Effects 0.000 claims description 4
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- QVFWZNCVPCJQOP-UHFFFAOYSA-N chloralodol Chemical compound CC(O)(C)CC(C)OC(O)C(Cl)(Cl)Cl QVFWZNCVPCJQOP-UHFFFAOYSA-N 0.000 claims 1
- 238000010924 continuous production Methods 0.000 claims 1
- 230000006641 stabilisation Effects 0.000 claims 1
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- 229930027917 kanamycin Natural products 0.000 description 19
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- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 19
- 229930182823 kanamycin A Natural products 0.000 description 19
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- -1 carbon compound pyruvate Chemical class 0.000 description 5
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- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
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- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 101001004672 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Probable L-lactate dehydrogenase Proteins 0.000 description 1
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- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
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- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0024554.8A GB0024554D0 (en) | 2000-10-06 | 2000-10-06 | Ethanol production |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA200302998B true ZA200302998B (en) | 2006-07-26 |
Family
ID=9900827
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA200302998A ZA200302998B (en) | 2000-10-06 | 2003-04-16 | Ethanol production |
Country Status (6)
Country | Link |
---|---|
US (3) | US20020081677A1 (de) |
CN (2) | CN101775363B (de) |
AT (1) | ATE509114T1 (de) |
DK (1) | DK1322775T3 (de) |
GB (1) | GB0024554D0 (de) |
ZA (1) | ZA200302998B (de) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4463200B2 (ja) * | 2003-03-12 | 2010-05-12 | 株式会社ミツカングループ本社 | 酢酸菌のアルコール脱水素酵素遺伝子 |
NZ563043A (en) * | 2005-05-04 | 2010-04-30 | Tmo Renewables Ltd | Thermophilic microorganisms with inactivated lactate dehydrogenase gene (LDH) for ethanol production |
UA100673C2 (uk) | 2006-07-21 | 2013-01-25 | Ксилеко, Инк. | Спосіб переробки целюлозної або лігноцелюлозної біомаси (варіанти) |
WO2009078973A2 (en) * | 2007-12-13 | 2009-06-25 | Glycos Biotechnologies, Incorporated | Microbial conversion of oils and fatty acids to high-value chemicals |
US20090246841A1 (en) * | 2008-03-26 | 2009-10-01 | Jamieson Andrew C | Methods and compositions for production of acetaldehyde |
NZ600509A (en) | 2009-12-29 | 2014-08-29 | Butamax Tm Advanced Biofuels | Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols |
CN102741388B (zh) * | 2010-01-26 | 2015-02-25 | 斯科尔生物燃料公司 | 一种生产和获取乙醇的方法及装置 |
CN114410562B (zh) * | 2022-02-08 | 2023-05-30 | 安徽工程大学 | 一种克雷伯氏菌工程菌及其在生产乙醇中的应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58147540A (ja) | 1982-02-26 | 1983-09-02 | Hitachi Ltd | 薄膜永久磁石の製造方法 |
US5482846A (en) * | 1988-08-31 | 1996-01-09 | University Of Florida | Ethanol production in Gram-positive microbes |
DE69028890T2 (de) | 1989-08-11 | 1997-02-27 | Gist Brocades Nv | Ergiebige Herstellung von Protease-Mutanten |
JP2931939B2 (ja) | 1992-07-10 | 1999-08-09 | 明治乳業株式会社 | Lactobacillus delbrueckii 種染色体への遺伝子組込み方法及び遺伝子組込み体 |
US5827515A (en) | 1995-08-30 | 1998-10-27 | Sandoz Ltd. | Bacillus thuringiensis sporulation gene |
GB0000185D0 (en) | 2000-01-06 | 2000-03-01 | Agrol Limited | Ethanol production |
-
2000
- 2000-10-06 GB GBGB0024554.8A patent/GB0024554D0/en not_active Ceased
-
2001
- 2001-10-05 CN CN200910204036.0A patent/CN101775363B/zh not_active Expired - Fee Related
- 2001-10-05 US US09/971,361 patent/US20020081677A1/en not_active Abandoned
- 2001-10-05 AT AT01972318T patent/ATE509114T1/de not_active IP Right Cessation
- 2001-10-05 DK DK01972318.8T patent/DK1322775T3/da active
- 2001-10-05 CN CNA018195660A patent/CN1798846A/zh active Pending
-
2003
- 2003-04-16 ZA ZA200302998A patent/ZA200302998B/en unknown
-
2005
- 2005-04-19 US US11/108,870 patent/US20050181492A1/en not_active Abandoned
-
2009
- 2009-02-24 US US12/391,366 patent/US8097460B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US20050181492A1 (en) | 2005-08-18 |
US20100190259A1 (en) | 2010-07-29 |
CN101775363B (zh) | 2016-08-03 |
GB0024554D0 (en) | 2000-11-22 |
US8097460B2 (en) | 2012-01-17 |
CN1798846A (zh) | 2006-07-05 |
CN101775363A (zh) | 2010-07-14 |
ATE509114T1 (de) | 2011-05-15 |
US20020081677A1 (en) | 2002-06-27 |
DK1322775T3 (da) | 2011-08-29 |
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