ZA200208311B - Expression-regulating sequences and expression products in the field of filamentous fungi chrysosporium. - Google Patents
Expression-regulating sequences and expression products in the field of filamentous fungi chrysosporium. Download PDFInfo
- Publication number
- ZA200208311B ZA200208311B ZA200208311A ZA200208311A ZA200208311B ZA 200208311 B ZA200208311 B ZA 200208311B ZA 200208311 A ZA200208311 A ZA 200208311A ZA 200208311 A ZA200208311 A ZA 200208311A ZA 200208311 B ZA200208311 B ZA 200208311B
- Authority
- ZA
- South Africa
- Prior art keywords
- chrysosporium
- strain
- polypeptide
- expression
- strains
- Prior art date
Links
- 241000123346 Chrysosporium Species 0.000 title claims description 93
- 230000014509 gene expression Effects 0.000 title claims description 60
- 108090000623 proteins and genes Proteins 0.000 claims description 88
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 84
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 84
- 229920001184 polypeptide Polymers 0.000 claims description 75
- 102000004169 proteins and genes Human genes 0.000 claims description 67
- 238000000034 method Methods 0.000 claims description 58
- 150000007523 nucleic acids Chemical class 0.000 claims description 39
- 102000004190 Enzymes Human genes 0.000 claims description 35
- 108090000790 Enzymes Proteins 0.000 claims description 35
- 229940088598 enzyme Drugs 0.000 claims description 35
- 230000002538 fungal effect Effects 0.000 claims description 32
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 29
- 238000004519 manufacturing process Methods 0.000 claims description 28
- 108010059892 Cellulase Proteins 0.000 claims description 26
- 230000001105 regulatory effect Effects 0.000 claims description 25
- 108091005804 Peptidases Proteins 0.000 claims description 21
- 239000004365 Protease Substances 0.000 claims description 20
- 150000001413 amino acids Chemical class 0.000 claims description 18
- 241000233866 Fungi Species 0.000 claims description 17
- 102000035195 Peptidases Human genes 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 17
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 claims description 16
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 16
- 210000004027 cell Anatomy 0.000 claims description 15
- 229940106157 cellulase Drugs 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 15
- 241000223259 Trichoderma Species 0.000 claims description 14
- 241000499912 Trichoderma reesei Species 0.000 claims description 12
- 230000001747 exhibiting effect Effects 0.000 claims description 12
- 238000003556 assay Methods 0.000 claims description 11
- 102000004157 Hydrolases Human genes 0.000 claims description 10
- 108090000604 Hydrolases Proteins 0.000 claims description 10
- 108010059820 Polygalacturonase Proteins 0.000 claims description 10
- 230000028327 secretion Effects 0.000 claims description 10
- 230000009466 transformation Effects 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 230000035772 mutation Effects 0.000 claims description 9
- 230000007935 neutral effect Effects 0.000 claims description 8
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 102000039446 nucleic acids Human genes 0.000 claims description 8
- 108090001060 Lipase Proteins 0.000 claims description 7
- 102000004882 Lipase Human genes 0.000 claims description 7
- 239000004367 Lipase Substances 0.000 claims description 7
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 7
- 235000019421 lipase Nutrition 0.000 claims description 7
- 108090000371 Esterases Proteins 0.000 claims description 6
- 238000009396 hybridization Methods 0.000 claims description 5
- 102000004316 Oxidoreductases Human genes 0.000 claims description 4
- 108090000854 Oxidoreductases Proteins 0.000 claims description 4
- 102000004357 Transferases Human genes 0.000 claims description 4
- 108090000992 Transferases Proteins 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims description 4
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 238000010367 cloning Methods 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- 108091035707 Consensus sequence Proteins 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 claims description 2
- 108700005088 Fungal Genes Proteins 0.000 claims description 2
- 108090000787 Subtilisin Proteins 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 claims description 2
- 229940088710 antibiotic agent Drugs 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 238000002742 combinatorial mutagenesis Methods 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 4
- 238000003776 cleavage reaction Methods 0.000 claims 4
- 239000002243 precursor Substances 0.000 claims 4
- 230000007017 scission Effects 0.000 claims 4
- 238000013459 approach Methods 0.000 claims 3
- 229930010796 primary metabolite Natural products 0.000 claims 3
- 210000001938 protoplast Anatomy 0.000 claims 3
- 238000005215 recombination Methods 0.000 claims 3
- 230000006798 recombination Effects 0.000 claims 3
- 229930182555 Penicillin Natural products 0.000 claims 2
- 150000007524 organic acids Chemical class 0.000 claims 2
- 235000005985 organic acids Nutrition 0.000 claims 2
- 238000012216 screening Methods 0.000 claims 2
- 229930000044 secondary metabolite Natural products 0.000 claims 2
- 230000004083 survival effect Effects 0.000 claims 2
- 238000011426 transformation method Methods 0.000 claims 2
- QRBLKGHRWFGINE-UGWAGOLRSA-N 2-[2-[2-[[2-[[4-[[2-[[6-amino-2-[3-amino-1-[(2,3-diamino-3-oxopropyl)amino]-3-oxopropyl]-5-methylpyrimidine-4-carbonyl]amino]-3-[(2r,3s,4s,5s,6s)-3-[(2s,3r,4r,5s)-4-carbamoyl-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)- Chemical compound N=1C(C=2SC=C(N=2)C(N)=O)CSC=1CCNC(=O)C(C(C)=O)NC(=O)C(C)C(O)C(C)NC(=O)C(C(O[C@H]1[C@@]([C@@H](O)[C@H](O)[C@H](CO)O1)(C)O[C@H]1[C@@H]([C@](O)([C@@H](O)C(CO)O1)C(N)=O)O)C=1NC=NC=1)NC(=O)C1=NC(C(CC(N)=O)NCC(N)C(N)=O)=NC(N)=C1C QRBLKGHRWFGINE-UGWAGOLRSA-N 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229930186147 Cephalosporin Natural products 0.000 claims 1
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 claims 1
- 102100036238 Dihydropyrimidinase Human genes 0.000 claims 1
- 102000004961 Furin Human genes 0.000 claims 1
- 108090001126 Furin Proteins 0.000 claims 1
- 206010064571 Gene mutation Diseases 0.000 claims 1
- 108091027305 Heteroduplex Proteins 0.000 claims 1
- 101710198130 NADPH-cytochrome P450 reductase Proteins 0.000 claims 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims 1
- LTQCLFMNABRKSH-UHFFFAOYSA-N Phleomycin Natural products N=1C(C=2SC=C(N=2)C(N)=O)CSC=1CCNC(=O)C(C(O)C)NC(=O)C(C)C(O)C(C)NC(=O)C(C(OC1C(C(O)C(O)C(CO)O1)OC1C(C(OC(N)=O)C(O)C(CO)O1)O)C=1NC=NC=1)NC(=O)C1=NC(C(CC(N)=O)NCC(N)C(N)=O)=NC(N)=C1C LTQCLFMNABRKSH-UHFFFAOYSA-N 0.000 claims 1
- 108010035235 Phleomycins Proteins 0.000 claims 1
- 108700005078 Synthetic Genes Proteins 0.000 claims 1
- 241001515886 Tolypocladium geodes Species 0.000 claims 1
- 230000008238 biochemical pathway Effects 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 229940124587 cephalosporin Drugs 0.000 claims 1
- 150000001780 cephalosporins Chemical class 0.000 claims 1
- 238000012411 cloning technique Methods 0.000 claims 1
- 238000010276 construction Methods 0.000 claims 1
- 238000007796 conventional method Methods 0.000 claims 1
- 108091022884 dihydropyrimidinase Proteins 0.000 claims 1
- 238000001952 enzyme assay Methods 0.000 claims 1
- 238000003018 immunoassay Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 238000010369 molecular cloning Methods 0.000 claims 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims 1
- 238000005457 optimization Methods 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 229940049954 penicillin Drugs 0.000 claims 1
- 150000002960 penicillins Chemical class 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 230000004952 protein activity Effects 0.000 claims 1
- 238000000159 protein binding assay Methods 0.000 claims 1
- 238000012552 review Methods 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
- 239000007858 starting material Substances 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 62
- 108010076504 Protein Sorting Signals Proteins 0.000 description 18
- 241000228212 Aspergillus Species 0.000 description 16
- 239000002028 Biomass Substances 0.000 description 13
- 108010084185 Cellulases Proteins 0.000 description 10
- 102000005575 Cellulases Human genes 0.000 description 10
- 241000228245 Aspergillus niger Species 0.000 description 9
- 235000001014 amino acid Nutrition 0.000 description 9
- 241001674013 Chrysosporium lucknowense Species 0.000 description 8
- 108700010070 Codon Usage Proteins 0.000 description 8
- 108010065511 Amylases Proteins 0.000 description 7
- 102000013142 Amylases Human genes 0.000 description 7
- 101150092493 CREA gene Proteins 0.000 description 7
- 235000019418 amylase Nutrition 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 239000004382 Amylase Substances 0.000 description 6
- 241000223218 Fusarium Species 0.000 description 6
- 101710091977 Hydrophobin Proteins 0.000 description 6
- 241000235648 Pichia Species 0.000 description 6
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 6
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 6
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 5
- 241000223198 Humicola Species 0.000 description 5
- 241000235395 Mucor Species 0.000 description 5
- 241000221960 Neurospora Species 0.000 description 5
- 241001494489 Thielavia Species 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 108010005774 beta-Galactosidase Proteins 0.000 description 5
- 102000005936 beta-Galactosidase Human genes 0.000 description 5
- 235000019419 proteases Nutrition 0.000 description 5
- 241000754533 Bettsia alvei Species 0.000 description 4
- 241001453604 Bettsia fastidia Species 0.000 description 4
- 101710098247 Exoglucanase 1 Proteins 0.000 description 4
- 102100022624 Glucoamylase Human genes 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241000235402 Rhizomucor Species 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 241001313536 Thermothelomyces thermophila Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000003197 catalytic effect Effects 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000002703 mutagenesis Methods 0.000 description 4
- 231100000350 mutagenesis Toxicity 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 3
- 241000351920 Aspergillus nidulans Species 0.000 description 3
- 240000006439 Aspergillus oryzae Species 0.000 description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 241000228143 Penicillium Species 0.000 description 3
- 241000235403 Rhizomucor miehei Species 0.000 description 3
- 241000235070 Saccharomyces Species 0.000 description 3
- 241001495429 Thielavia terrestris Species 0.000 description 3
- 241001149964 Tolypocladium Species 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 230000001627 detrimental effect Effects 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 125000003147 glycosyl group Chemical group 0.000 description 3
- 210000004276 hyalin Anatomy 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 101150034227 xyl1 gene Proteins 0.000 description 3
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
- 101100272859 Arabidopsis thaliana BXL1 gene Proteins 0.000 description 2
- 241000203069 Archaea Species 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 2
- 241000228138 Emericella Species 0.000 description 2
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000228172 Penicillium canescens Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241001085826 Sporotrichum Species 0.000 description 2
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 2
- 241000228341 Talaromyces Species 0.000 description 2
- 241000223262 Trichoderma longibrachiatum Species 0.000 description 2
- 108010048241 acetamidase Proteins 0.000 description 2
- 229940025131 amylases Drugs 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- -1 chitanases) Proteins 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 208000021267 infertility disease Diseases 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000001937 non-anti-biotic effect Effects 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 235000019833 protease Nutrition 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 101150016309 trpC gene Proteins 0.000 description 2
- SBKVPJHMSUXZTA-MEJXFZFPSA-N (2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-5-amino-2-[[2-[[(2S)-1-[(2S)-6-amino-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-indol-3-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-methylpentanoyl]amino]-5-oxopentanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]pyrrolidine-2-carbonyl]amino]acetyl]amino]-5-oxopentanoyl]pyrrolidine-2-carbonyl]amino]-4-methylsulfanylbutanoyl]amino]-3-(4-hydroxyphenyl)propanoic acid Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 SBKVPJHMSUXZTA-MEJXFZFPSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 101150029062 15 gene Proteins 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- 108010011619 6-Phytase Proteins 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000004400 Aminopeptidases Human genes 0.000 description 1
- 108090000915 Aminopeptidases Proteins 0.000 description 1
- 108010037870 Anthranilate Synthase Proteins 0.000 description 1
- 241001313476 Arthroderma ciferrii Species 0.000 description 1
- 241001336563 Arthroderma vespertilii Species 0.000 description 1
- 108010017640 Aspartic Acid Proteases Proteins 0.000 description 1
- 102000004580 Aspartic Acid Proteases Human genes 0.000 description 1
- 241001513093 Aspergillus awamori Species 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 101710130006 Beta-glucanase Proteins 0.000 description 1
- 108700038091 Beta-glucanases Proteins 0.000 description 1
- 102100032487 Beta-mannosidase Human genes 0.000 description 1
- 102000005367 Carboxypeptidases Human genes 0.000 description 1
- 108010006303 Carboxypeptidases Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241000221955 Chaetomium Species 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 241001336579 Chrysosporium carmichaelii Species 0.000 description 1
- 241001336578 Chrysosporium europae Species 0.000 description 1
- 241001313448 Chrysosporium indicum Species 0.000 description 1
- 241000985909 Chrysosporium keratinophilum Species 0.000 description 1
- 241001674015 Chrysosporium lobatum Species 0.000 description 1
- 241001674010 Chrysosporium mephiticum Species 0.000 description 1
- 241001556045 Chrysosporium merdarium Species 0.000 description 1
- 241000393428 Chrysosporium merdarium var. roseum Species 0.000 description 1
- 241000131962 Chrysosporium parvum Species 0.000 description 1
- 241001556043 Chrysosporium pilosum Species 0.000 description 1
- 241001154026 Chrysosporium pseudomerdarium Species 0.000 description 1
- 241000080524 Chrysosporium queenslandicum Species 0.000 description 1
- 241001556041 Chrysosporium sulfureum Species 0.000 description 1
- 241001674001 Chrysosporium tropicum Species 0.000 description 1
- 241001336565 Chrysosporium undulatum Species 0.000 description 1
- 241001556036 Chrysosporium vallenarense Species 0.000 description 1
- 241000355696 Chrysosporium zonatum Species 0.000 description 1
- 208000032544 Cicatrix Diseases 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241000198691 Cleisostoma filiforme Species 0.000 description 1
- 241001252397 Corynascus Species 0.000 description 1
- 241001503016 Ctenomyces Species 0.000 description 1
- 241001262649 Emmonsia crescens Species 0.000 description 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 108050008938 Glucoamylases Proteins 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- 101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000167881 Hirundo Species 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108010054377 Mannosidases Proteins 0.000 description 1
- 102000001696 Mannosidases Human genes 0.000 description 1
- 108010038049 Mating Factor Proteins 0.000 description 1
- 241000226677 Myceliophthora Species 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 102000007981 Ornithine carbamoyltransferase Human genes 0.000 description 1
- 101710113020 Ornithine transcarbamylase, mitochondrial Proteins 0.000 description 1
- 102100037214 Orotidine 5'-phosphate decarboxylase Human genes 0.000 description 1
- 108010055012 Orotidine-5'-phosphate decarboxylase Proteins 0.000 description 1
- 101710118538 Protease Proteins 0.000 description 1
- 240000000296 Sabal minor Species 0.000 description 1
- 241000223255 Scytalidium Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- 241000228178 Thermoascus Species 0.000 description 1
- 241000223260 Trichoderma harzianum Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000409279 Xerochrysium dermatitidis Species 0.000 description 1
- 241001453498 Xerochrysium xerophilum Species 0.000 description 1
- 241001556033 [Chrysosporium] synchronum Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108010030291 alpha-Galactosidase Proteins 0.000 description 1
- 102000005840 alpha-Galactosidase Human genes 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 101150008194 argB gene Proteins 0.000 description 1
- 229940091771 aspergillus fumigatus Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 108010055059 beta-Mannosidase Proteins 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 108010083912 bleomycin N-acetyltransferase Proteins 0.000 description 1
- 239000001058 brown pigment Substances 0.000 description 1
- 108010085318 carboxymethylcellulase Proteins 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 108020001778 catalytic domains Proteins 0.000 description 1
- 238000010960 commercial process Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 235000021474 generally recognized As safe (food) Nutrition 0.000 description 1
- 235000021473 generally recognized as safe (food ingredients) Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 101150087371 gpd1 gene Proteins 0.000 description 1
- 239000001056 green pigment Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229940094991 herring sperm dna Drugs 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 108010059345 keratinase Proteins 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 101150039489 lysZ gene Proteins 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000012666 negative regulation of transcription by glucose Effects 0.000 description 1
- 101150095344 niaD gene Proteins 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
- KCRZDTROFIOPBP-UHFFFAOYSA-N phosphono 2,3-dihydroxypropanoate Chemical compound OCC(O)C(=O)OP(O)(O)=O KCRZDTROFIOPBP-UHFFFAOYSA-N 0.000 description 1
- 239000001965 potato dextrose agar Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 101150054232 pyrG gene Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 108010038196 saccharide-binding proteins Proteins 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000003584 silencer Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000000196 viscometry Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Dairy Products (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00201343 | 2000-04-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA200208311B true ZA200208311B (en) | 2003-10-15 |
Family
ID=8171350
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA200208311A ZA200208311B (en) | 2000-04-13 | 2002-10-15 | Expression-regulating sequences and expression products in the field of filamentous fungi chrysosporium. |
Country Status (22)
Country | Link |
---|---|
US (2) | US7906309B2 (cs) |
EP (2) | EP1276876B1 (cs) |
JP (3) | JP4922524B2 (cs) |
KR (1) | KR100903780B1 (cs) |
CN (1) | CN100420753C (cs) |
AT (1) | ATE358724T1 (cs) |
AU (1) | AU782105B2 (cs) |
BR (1) | BR0110090A (cs) |
CA (1) | CA2405954C (cs) |
CZ (1) | CZ303980B6 (cs) |
DE (1) | DE60127661T2 (cs) |
DK (1) | DK1276876T3 (cs) |
ES (1) | ES2283402T3 (cs) |
HU (1) | HUP0300469A2 (cs) |
IL (1) | IL152272A (cs) |
MX (1) | MXPA02010155A (cs) |
NO (1) | NO20024945L (cs) |
NZ (1) | NZ521990A (cs) |
PL (1) | PL207374B1 (cs) |
RU (1) | RU2272835C2 (cs) |
WO (1) | WO2001079507A2 (cs) |
ZA (1) | ZA200208311B (cs) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
EA005682B1 (ru) * | 1998-10-06 | 2005-04-28 | Марк Аарон Эмалфарб | Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов |
CZ303980B6 (cs) * | 2000-04-13 | 2013-07-31 | Dyadic International (Usa), Inc. | Expresi regulující sekvence nukleové kyseliny a produkty exprese v oblasti vláknitých plísní |
ES2397267T3 (es) | 2001-06-26 | 2013-03-05 | Novozymes A/S | Polipéptidos con actividad de celobiohidrolasa I y polinucleótidos que codifican los mismos |
WO2006012904A1 (en) * | 2004-08-06 | 2006-02-09 | Novozymes A/S | Polypeptides of botryosphaeria rhodina |
DK1989302T3 (en) * | 2006-02-14 | 2018-07-23 | Bp Corp North America Inc | XYLANASES, NUCLEIC ACID CODING THEM AND PROCEDURES FOR THEIR PREPARATION AND USE |
US9862956B2 (en) | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
EP2505651A3 (en) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
CN101855345A (zh) | 2007-05-10 | 2010-10-06 | 诺维信股份有限公司 | 用于增强含纤维素材料的降解或转化的组合物和方法 |
US8551751B2 (en) | 2007-09-07 | 2013-10-08 | Dyadic International, Inc. | BX11 enzymes having xylosidase activity |
US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
EP2408910B1 (en) | 2009-03-16 | 2018-04-25 | Danisco US Inc. | Chrysosporium lucknowense protein production system |
AU2010241099A1 (en) * | 2009-04-22 | 2011-10-27 | Dsm Ip Assets B.V. | Process for the production of a recombinant polypeptide of interest |
WO2010129287A2 (en) | 2009-04-27 | 2010-11-11 | The Board Of Trustees Of The University Of Illinois | Hemicellulose-degrading enzymes |
DK2483415T3 (en) | 2009-09-30 | 2015-02-09 | Codexis Inc | RECOMBINANT C1 B-glucosidase FOR MANUFACTURE OF sugars from cellulosic BIOMASS |
JP5735775B2 (ja) * | 2009-10-21 | 2015-06-17 | 花王株式会社 | 改変プロモーター |
CN101831448A (zh) * | 2010-03-26 | 2010-09-15 | 武汉大学 | 一种植物三磷酸甘油醛脱氢酶基因及制备方法和应用 |
US9493802B2 (en) | 2010-08-20 | 2016-11-15 | Codexis, Inc. | Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose |
WO2012024662A2 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Expression constructs comprising fungal promoters |
JP2013539971A (ja) * | 2010-09-15 | 2013-10-31 | ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア | Trichodermareeseiのエンドグルカナーゼiの好熱性変異体 |
US9868958B2 (en) * | 2011-04-22 | 2018-01-16 | Danisco Us Inc. | Filamentous fungi having an altered viscosity phenotype |
CN103517916B (zh) * | 2011-04-22 | 2017-05-10 | 丹尼斯科美国公司 | 具有粘度改变表型的丝状真菌 |
WO2013048661A1 (en) | 2011-09-30 | 2013-04-04 | Codexis, Inc. | Fungal proteases |
US9249418B2 (en) | 2011-10-31 | 2016-02-02 | Bp Corporation North America Inc. | Use of plant promoters in filamentous fungi |
CA2851308A1 (en) | 2011-10-31 | 2013-05-10 | Bp Corporation North America Inc. | Use of mammalian promoters in filamentous fungi |
WO2014070856A2 (en) | 2012-11-02 | 2014-05-08 | Bp Corporation North America Inc. | Thermotolerant beta-glucosidase variants |
WO2014145768A2 (en) | 2013-03-15 | 2014-09-18 | Bp Corporation North America Inc. | Use of non-fungal 5' utrs in filamentous fungi |
KR101598824B1 (ko) | 2015-09-25 | 2016-03-02 | 경인엔지니어링 주식회사 | 역 v형 루버 구조를 이용한 배전반 방열장치 |
EP3672630A4 (en) * | 2017-08-21 | 2021-06-02 | Dyadic International Inc. | MANUFACTURING OF A FLU VACCINE IN MYCELIOPHTHORA THERMOPHILA |
WO2024132947A1 (en) * | 2022-12-19 | 2024-06-27 | Basf Se | New cellulase promoters for fungal protein production |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK115890D0 (da) * | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
GB9400623D0 (en) * | 1994-01-14 | 1994-03-09 | Univ Leeds | Exploitation of the cellulase enzyme complex of neurospora |
GB2289218A (en) * | 1994-05-06 | 1995-11-15 | Merck & Co Inc | Inhibition of TNFalpha production with agonists of the A2b subtype of the adenosine receptor |
US5550020A (en) * | 1994-07-08 | 1996-08-27 | Visible Genetics Inc. | Method, reagents and kit for diagnosis and targeted screening for retinoblastoma |
JP3107977B2 (ja) * | 1994-08-29 | 2000-11-13 | 明治製菓株式会社 | 新規セルラーゼおよびその遺伝子 |
WO1997013853A2 (en) * | 1995-10-13 | 1997-04-17 | Gist-Brocades B.V. | Protein detection |
DE69704845T2 (de) | 1996-01-26 | 2001-12-20 | Novozymes A/S, Bagsvaerd | Herstellung von toilettenpapier |
US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
US6001595A (en) * | 1996-11-29 | 1999-12-14 | Rohm Enzyme GmbH | Promoters and uses thereof |
WO1999025735A1 (en) * | 1997-11-19 | 1999-05-27 | Microbia, Inc. | Chimeric pre-activated transcription factors |
EA005682B1 (ru) * | 1998-10-06 | 2005-04-28 | Марк Аарон Эмалфарб | Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов |
US6121034A (en) * | 1999-05-13 | 2000-09-19 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Coniothyrium minitans xylanase gene Cxy1 |
WO2001025468A1 (en) * | 1999-10-06 | 2001-04-12 | Mark Aaron Emalfarb | High-throughput screening of expressed dna libraries in filamentous fungi |
CZ303980B6 (cs) * | 2000-04-13 | 2013-07-31 | Dyadic International (Usa), Inc. | Expresi regulující sekvence nukleové kyseliny a produkty exprese v oblasti vláknitých plísní |
DK3219806T3 (da) * | 2004-03-25 | 2020-07-13 | Novozymes Inc | Fremgangsmåder til nedbrydning eller omdannelse af plantecellevægspolysaccharider |
-
2001
- 2001-04-17 CZ CZ20023435A patent/CZ303980B6/cs not_active IP Right Cessation
- 2001-04-17 HU HU0300469A patent/HUP0300469A2/hu unknown
- 2001-04-17 KR KR1020027014055A patent/KR100903780B1/ko not_active IP Right Cessation
- 2001-04-17 US US10/257,629 patent/US7906309B2/en not_active Expired - Fee Related
- 2001-04-17 CA CA2405954A patent/CA2405954C/en not_active Expired - Fee Related
- 2001-04-17 IL IL152272A patent/IL152272A/en not_active IP Right Cessation
- 2001-04-17 MX MXPA02010155A patent/MXPA02010155A/es active IP Right Grant
- 2001-04-17 AT AT01923991T patent/ATE358724T1/de not_active IP Right Cessation
- 2001-04-17 EP EP01923991A patent/EP1276876B1/en not_active Expired - Lifetime
- 2001-04-17 PL PL358491A patent/PL207374B1/pl not_active IP Right Cessation
- 2001-04-17 WO PCT/NL2001/000301 patent/WO2001079507A2/en active IP Right Grant
- 2001-04-17 AU AU50663/01A patent/AU782105B2/en not_active Ceased
- 2001-04-17 CN CNB018110681A patent/CN100420753C/zh not_active Expired - Fee Related
- 2001-04-17 NZ NZ521990A patent/NZ521990A/en unknown
- 2001-04-17 JP JP2001577490A patent/JP4922524B2/ja not_active Expired - Fee Related
- 2001-04-17 EP EP07102273A patent/EP1854888A3/en not_active Withdrawn
- 2001-04-17 RU RU2002130278/13A patent/RU2272835C2/ru not_active IP Right Cessation
- 2001-04-17 ES ES01923991T patent/ES2283402T3/es not_active Expired - Lifetime
- 2001-04-17 DE DE60127661T patent/DE60127661T2/de not_active Expired - Lifetime
- 2001-04-17 BR BR0110090-4A patent/BR0110090A/pt not_active IP Right Cessation
- 2001-04-17 DK DK01923991T patent/DK1276876T3/da active
-
2002
- 2002-10-14 NO NO20024945A patent/NO20024945L/no not_active Application Discontinuation
- 2002-10-15 ZA ZA200208311A patent/ZA200208311B/en unknown
-
2011
- 2011-03-14 US US13/046,772 patent/US20130143271A1/en not_active Abandoned
- 2011-07-15 JP JP2011156864A patent/JP2011234729A/ja active Pending
-
2014
- 2014-06-19 JP JP2014126246A patent/JP2014236730A/ja active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ZA200208311B (en) | Expression-regulating sequences and expression products in the field of filamentous fungi chrysosporium. | |
US8871493B2 (en) | Transformation system in the field of filamentous fungal hosts | |
EP1272669B1 (en) | High-throughput screening of expressed dna libraries in filamentous fungi | |
WO2001025468A1 (en) | High-throughput screening of expressed dna libraries in filamentous fungi | |
CN107109424A (zh) | 真菌宿主菌株、dna构建体及使用方法 | |
RU2238974C2 (ru) | Фрагмент днк мицелиального гриба penicillium verruculosum, кодирующий синтез секретируемой эндоглюканазы iii и группа штаммов penicillium canescens, синтезирующих эндоглюканазу iii penicillium verruculosum, сконструированных методами трансформации и генетической инженерии на основе этого фрагмента днк | |
MXPA01003462A (en) | Transformation system in the field of filamentous fungal hosts | |
BRPI0110090B1 (pt) | Construction of nucleic acid, process for producing a polipeptde, and microbial recombinant cepa |