WO2023210168A1 - BOISSON AU CAFÉ NOIR CONTENANT DE L'ACIDE γ-AMINOBUTYRIQUE - Google Patents

BOISSON AU CAFÉ NOIR CONTENANT DE L'ACIDE γ-AMINOBUTYRIQUE Download PDF

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Publication number
WO2023210168A1
WO2023210168A1 PCT/JP2023/008262 JP2023008262W WO2023210168A1 WO 2023210168 A1 WO2023210168 A1 WO 2023210168A1 JP 2023008262 W JP2023008262 W JP 2023008262W WO 2023210168 A1 WO2023210168 A1 WO 2023210168A1
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WO
WIPO (PCT)
Prior art keywords
coffee
gaba
beverage
black coffee
black
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PCT/JP2023/008262
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English (en)
Japanese (ja)
Inventor
新 河野
鷹明 谷
Original Assignee
サントリーホールディングス株式会社
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Publication of WO2023210168A1 publication Critical patent/WO2023210168A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F5/00—Coffee; Coffee substitutes; Preparations thereof
    • A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee

Definitions

  • the present invention relates to a black coffee beverage with a pH of 6.0 to 7.5.
  • Coffee drinks are highly palatable and are widely enjoyed around the world. Coffee beverages are provided to consumers in a wide variety of ways.For example, in addition to being served directly at home or at a restaurant (so-called regular coffee), coffee beverages are provided in retail stores or vending machines in RTD (READY TO DRINK) formats. It is also available in the form of packaged coffee drinks.
  • regular coffee in addition to being served directly at home or at a restaurant (so-called regular coffee), coffee beverages are provided in retail stores or vending machines in RTD (READY TO DRINK) formats. It is also available in the form of packaged coffee drinks.
  • RTD READY TO DRINK
  • regular coffee has a slightly acidic pH of about 5, and some have a pH of less than 5.
  • the pH of powdered coffee (so-called instant coffee) diluted with predetermined hot water or water is also about 5.
  • the sourness of coffee indicates the fruitiness derived from coffee berries, and is considered to be one of the attractive features of coffee, which is linked to its freshness and sharpness.
  • a pH adjuster such as baking soda (sodium bicarbonate) is usually added to suppress the pH drop that occurs during heat sterilization or long-term storage under heating conditions.
  • the pH is adjusted to be in the neutral range of 5.5 or more, preferably 6.0 or more.
  • GABA ⁇ -aminobutyric acid
  • the present invention aims to provide a neutral range black coffee beverage with improved flavor.
  • the present inventors found that by containing a specific amount of ⁇ -aminobutyric acid, it is possible to impart a sour taste peculiar to coffee drinks to a black coffee drink in the neutral range.
  • the inventors have discovered that it is possible to provide a neutral black coffee beverage with improved flavor, and have completed the present invention.
  • the present invention includes, but is not limited to, the following aspects.
  • [1] A black coffee beverage containing 60 to 800 mg/kg of ⁇ -aminobutyric acid and having a pH of 6.0 to 7.5.
  • [2] The black coffee beverage according to [1], wherein the content of furfuryl acetate is 2.0 ⁇ g/kg or more.
  • [3] The black coffee beverage according to [1] or [2], which is a packaged beverage.
  • the present invention solves the problem caused by adjusting the pH to a neutral range in order to package regular coffee, which has an acidic liquid, and targets black coffee beverages in the neutral range.
  • the black coffee beverage as used herein refers to a coffee beverage that does not use dairy products.
  • milk-containing coffee beverages also have a neutral liquid level, but milk-containing coffee beverages are not subject to the present invention because their sharp, slightly sour taste, like that of black coffee beverages, is not important.
  • the black coffee beverage may be a sugar-free type that does not use sugar or a sweetened type that uses sugar, but from the viewpoint of easily enjoying the effects of the present invention, it is preferable that the sweetness level is 1 or less. Preferably, no sugars are used.
  • the sweetness level is a measure indicating the intensity of sweetness, and is a relative ratio when the sweetness of 1% by mass of sucrose (20° C.) is set to 1.
  • the degree of sweetness of a beverage is determined by converting the amount (mass concentration) of each sweet component contained in the beverage into the equivalent amount of sucrose based on the relative ratio of the sweetness of the sweet component to the sweetness of sucrose, and then It can be determined by summing up the sucrose equivalent amounts of all sweet ingredients contained in.
  • the relative ratio of the sweetness of various sweetness components to the sweetness 1 of sucrose can be determined from a known sugar sweetness conversion table (McMurray Organic Chemistry (7th edition) p. 988).
  • the coffee beverage is a beverage mainly containing roasted coffee bean extract, and specifically, the amount of roasted coffee beans used per 100g of coffee beverage is 1g or more in terms of green beans.
  • the weight is preferably 2.5 g or more, more preferably 5 g or more, and even more preferably 5 g or more.
  • the green bean equivalent value is that 1g of roasted coffee beans is equivalent to 1.3g of green coffee beans (revised new edition, Soft Drinks, supervised by: National Soft Drink Industry Association, published by: Korin, 1989) Published on December 25th, page 421).
  • the type of coffee beans used in the black coffee beverage of the present invention is not particularly limited.
  • cultivated tree species include Arabica, Robusta, and Liberica, and examples of coffee varieties include Mocha, Brazil, Colombia, Guatemala, Blue Mountain, Kona, Mandhelin, and Kilimanjaro.
  • One type of coffee beans may be used, or a mixture of multiple types may be used.
  • coffee beans are roasted to obtain roasted coffee beans, and the roasted coffee beans are extracted to obtain a roasted coffee bean extract, which is used in a beverage.
  • the coffee bean extract referred to in this specification includes a coffee bean extract and a powder obtained by drying and powdering the extract. Coffee bean extract can be produced by conventional methods.
  • roasting roasted coffee beans there are no particular restrictions on the method of roasting roasted coffee beans, and there are no restrictions on the roasting temperature or roasting environment, and any conventional method can be used. Furthermore, there are no restrictions on the method of extraction from roasted coffee beans; for example, using water or hot water (0 to 100°C) from roasted coffee beans that have been ground into coarse, medium, or fine grinds. A method of extracting for 10 seconds to 30 minutes is mentioned. Extraction methods include drip, siphon, boiling, jet, and continuous methods.
  • the neutral region of the black coffee beverage refers to a pH of the beverage at 20° C. of 6.0 or higher. From the viewpoint of quality stability during storage, it is preferably 6.1 or more, more preferably 6.2 or more, even more preferably 6.3 or more, particularly preferably 6.4 or more. From the viewpoint of conspicuousness of the effect of the present invention, lower pH is preferable. From this, the upper limit of pH is about 7.5, preferably 7.3 or less, more preferably 7.0 or less, and still more preferably 6.8 or less.
  • the present invention aims to add ⁇ -aminobutyric acid (hereinafter abbreviated as "GABA”) to the unique fruit-like acidity of coffee, which tends to be lacking in black coffee drinks adjusted to a neutral range. It is intended to be strengthened by Even in black coffee drinks with a pH of less than 6.0, the acidity is enhanced by adding GABA, but it does not have the fruit-like sourness unique to coffee, but has a strong, stimulating sourness like acetic acid, which has a negative impact on the flavor. This is undesirable as it may cause harmful effects.
  • GABA ⁇ -aminobutyric acid
  • a pH adjuster is used to adjust the pH of the black coffee beverage of the present invention.
  • the pH adjuster is not limited as long as it is a substance that shows alkalinity when dissolved in water, and specific examples include baking soda (sodium hydrogen carbonate), sodium carbonate, potassium carbonate, sodium hydroxide, potassium hydroxide, and phosphoric acid. Examples include trisodium and tripotassium phosphate.
  • the present invention improves the flavor of a black coffee beverage adjusted to a neutral range by adding ⁇ -aminobutyric acid (GABA).
  • GABA ⁇ -aminobutyric acid
  • the flavor improvement as used in the present invention refers to the effect of imparting the acidity characteristic of coffee drinks, which tends to be lacking in the neutral region, and compared to the case where GABA is not added, weakly acidic regular coffee This means that it has a flavor similar to that of Japanese radish, specifically a taste with a sharp, slightly sour taste.
  • GABA is a type of amino acid that is widely contained in vegetables, fruits, grains, fermented foods, etc.
  • the origin of GABA used in the present invention is not particularly limited, and for example, GABA extracted from vegetables, fruits, grains, etc., GABA produced by fermentation, GABA obtained by organic synthesis, etc. can be used. From the viewpoint of minimizing the influence on the flavor of the beverage itself, it is preferable to use a purified GABA product containing GABA in an amount of 80% by mass or more, preferably 85% by mass or more, more preferably 90% by mass or more. Various forms of the purified product can be used, such as solid, aqueous solution, and slurry.
  • Commercially available purified products of GABA include, for example, GABA 100% Pure Powder (NOW FOODS Co., Ltd.), Oryza GABA (registered trademark) Extract HC-90 (Oryza Yuka Co., Ltd.), and the like.
  • GABA is added to the black coffee beverage so that the concentration of GABA in the beverage is 60 mg/kg or more, preferably 100 mg/kg or more, more preferably 150 mg/kg or more, particularly preferably 200 mg/kg or more. do.
  • the content of GABA in the beverage is preferably 800 mg/kg or less, more preferably 700 mg/kg or less, and even more preferably 600 mg/kg or less.
  • the content of GABA can be measured using an amino acid analyzer.
  • the black coffee beverage of the present invention contains furfuryl acetate.
  • Furfuryl acetate is an aroma component known as a ripe fruit-like aroma component.
  • the present invention imparts or enhances the subtle sourness that tends to be lacking in black coffee drinks adjusted to a neutral range by adding GABA, but when the coffee drink contains a specific amount of furfuryl acetate. It was discovered that the sourness unique to coffee beverages imparted/enhanced by GABA can be perceived more as a fruit-like natural sourness unique to coffee.
  • the furfuryl acetate content in the black coffee beverage of the present invention is preferably 2.0 ⁇ g/kg or more, more preferably 5.0 ⁇ g/kg or more, even more preferably 7.0 ⁇ g/kg or more, and particularly preferably 10 ⁇ g/kg or more.
  • the upper limit of the furfuryl acetate content is preferably 200 ⁇ g/kg or less, more preferably 100 ⁇ g/kg or less.
  • Furfuryl acetate content can be quantified by gas chromatography mass spectrometry (GC/MS analysis).
  • the furfuryl acetate content may be added as a component derived from coffee beans (coffee bean extract, ground coffee beans, etc.) or as a flavor component.
  • the beverage of the present invention contains a roasted coffee bean extract, a pH adjuster, and GABA, but other ingredients such as flavoring agents, sugars, etc. are included as long as they do not deviate from the intended purpose of the present invention.
  • a sweetening component components commonly added to black coffee beverages, such as antioxidants and quality stabilizers (emulsifiers, thickening stabilizers, etc.), can be added as appropriate.
  • a packaged beverage is one that is sealed after the beverage is filled into a container.
  • containers include conventional packaging containers such as molded containers containing polyethylene terephthalate as a main component (so-called PET bottles), metal cans, paper containers composited with metal foil or plastic film, and bottles.
  • heat sterilization may be performed.
  • the conditions for the heat sterilization treatment can be selected, for example, from a method that provides the same effect as the conditions stipulated in the Food Sanitation Act, and specifically, from 60 to 150°C, preferably from 90 to 150°C, or from 90 to 150°C.
  • the temperature is 110 to 150° C.
  • the time can be selected in the range of 1 second to 60 minutes, preferably 1 second to 30 minutes. More specifically, when a heat-resistant container (metal can, glass, etc.) is used as the container, retort sterilization (110 to 140°C, for 1 to several tens of minutes depending on the temperature) may be performed.
  • the prepared liquid when using a non-heat resistant container (PET bottle, paper container, etc.) as a container, for example, the prepared liquid should be sterilized at high temperature for a short period of time (UHT sterilization: 110 to 150°C, depending on the temperature) using a plate heat exchanger, etc. After cooling to a certain temperature (for 1 to several tens of seconds), it can be aseptically filled into the non-heat resistant container.
  • a black coffee beverage that has been heat sterilized by severe heating conditions at a temperature exceeding 100° C. is a particularly preferred embodiment of the present invention because it significantly exhibits the intended effects of the present invention.
  • the volume of the beverage in the container is not particularly limited, but is, for example, about 150 mL to 1000 mL, preferably about 160 mL to 600 mL.
  • the black coffee beverage of the present invention can be produced in the same manner as a regular black coffee beverage, except that GABA is added as part of the raw materials. That is, it is manufactured by a process including a process of mixing roasted coffee bean extract and GABA to prepare a liquid mixture, and a process of blending a pH adjuster to adjust the pH of the final beverage to 6.0 to 7.5. be done. If the beverage is a packaged beverage, it includes a step of filling the container with the coffee mixture and a step of heating and sterilizing it.
  • the pH decreases due to heat sterilization.
  • the pH of the packaged beverage can be controlled by measuring in advance how much the pH decreases due to heat sterilization and adjusting the pH of the liquid preparation before heat sterilization.
  • the pH of the liquid preparation before heat sterilization should normally be adjusted to about 6.5 to 8.0.
  • the manufacturing method preferably includes a step of adjusting the content of furfuryl acetate in the beverage to 2.0 ⁇ g/kg or more.
  • ⁇ Analysis method of aroma components Put 5 ml of the sample solution into a 20 ml glass bottle with a screw (diameter 18 mm, manufactured by Gestel), seal it with a metal lid with a PTFE septum (manufactured by Gestel), and extract the aroma components using solid phase microextraction (SPME). I did it. Quantification was performed using the standard addition method using the peak area detected in the EIC mode of GC/MS. The equipment and conditions used are shown below.
  • ⁇ SPME fiber StableFlex/SS, 50/30 ⁇ m DVB/CAR/PDMS, (manufactured by Superco)
  • ⁇ Fully automatic volatile component extraction and introduction device MultiPurposeSampler MPS2XL (manufactured by Gestel)
  • MPS2XL manufactured by Gestel
  • Prewarming 40°C for 5 minutes
  • ⁇ Stirring None
  • ⁇ Volatile component extraction 40°C for 30 minutes
  • ⁇ Volatile component desorption time 3 minutes
  • ⁇ GC oven: GC7890A manufactured by Agilent Technologies
  • VF-WAXms 60m x 0.25mm. d.
  • df 0.50 ⁇ m (manufactured by Agilent Technologies) ⁇ GC temperature conditions: 40°C (5 minutes) ⁇ 5°C/min ⁇ 260°C (11 minutes) ⁇ Carrier gas: Helium, 1.2ml/min, constant flow rate mode ⁇ Injection: Splitless method ⁇ Inlet temperature: 250°C ⁇ Mass spectrometer: GC/MS Triple Quad7000 (manufactured by Agilent Technologies) ⁇ Ionization method: EI (70eV) ⁇ Measurement method: Scan measurement or simultaneous scan and SIM measurement ⁇ Scan parameters: m/z35 to 350.
  • a sodium hydroxide aqueous solution (100-fold dilution) was added to this beverage to obtain a black coffee beverage with a pH of 6.4.
  • GABA (purity of 99% or more) was added to this neutral range black coffee beverage so that the content of ⁇ -aminobutyric acid (GABA) was 30 to 800 mg/kg to prepare a GABA-containing black coffee beverage.
  • the sourness of these beverages was sensory evaluated using a GABA-free beverage at pH 6.4 as a control. The evaluation was performed by a panel of six experts.
  • the sourness of the drink with no GABA added (control) at pH 6.4 is 1 point, and the sourness of the black coffee drink (pH 5.7, GABA content: 0mg/kg) before adding NaOH is 3 points.
  • the scores were evaluated using the following five-point scale.
  • the quality evaluation standard was a two-level evaluation when drinking: (+): Good sourness with a fruit-like sourness that does not feel strange, (-): An unpleasant sourness that feels strange.
  • (+) Good sourness with a fruit-like sourness that does not feel strange
  • (-) An unpleasant sourness that feels strange.
  • GABA content 60 to 600 mg/kg
  • the strength of the fragrance was also evaluated.
  • the evaluation criteria are: (-): The scent is weak compared to the control, ( ⁇ ): Not different from the control (same level), (+): The scent is strong compared to the control. did. In the case of GABA content of 60 to 800 mg/kg, all 6 panel members evaluated it as ⁇ . In other words, even when GABA was added, the aroma did not change.
  • Experimental Example 2 Preparation of GABA-containing black coffee beverage (2) A GABA-containing black coffee beverage was prepared in the same manner as in Experimental Example 1, except that the amount of sodium hydroxide was changed and the pH was adjusted to 7.3 or 6.2. Similar to Experimental Example 1, the sourness of these beverages was sensory evaluated using a GABA-free beverage with a neutral pH range as a control.
  • Experimental Example 3 Preparation of GABA-containing black coffee drink (3)
  • a commercially available canned unsweetened black coffee drink type: coffee
  • raw material coffee (coffee beans (Brazil, Ethiopia, others)
  • This black coffee drink The pH was 5.8, and the furfuryl acetate content was 1.5 ⁇ g/kg.
  • a GABA-containing black coffee beverage with a pH of 6.2 was prepared and sensory evaluated.
  • GABA purity of 99% or more
  • GABA ⁇ -aminobutyric acid
  • furfuryl acetate was added.
  • Sodium hydrogen carbonate pH adjuster
  • a packaged black coffee beverage in a PET bottle was produced in the same manner except that GABA was not added.
  • These black coffee drinks contain 5.6g of roasted coffee bean extract in terms of green coffee beans per 100g of the drink, have a Brix value of 1.2-1.3%, a pH of 6.4, and a Furfuryl acetate content was 7.5 ⁇ g/kg.
  • the obtained black coffee drink in a PET bottle was subjected to a sensory evaluation by five expert panelists.
  • the panel evaluated whether there was a difference in sourness between the paired drinks, and if so, which drink felt more sour.
  • the results are shown in Table 5.
  • a black coffee drink in a neutral range pH 6.4
  • the majority of panels evaluated that adding GABA to the drink so that the content in the drink was 60 mg/kg or more increased the acidity.
  • GABA was added at a concentration of 100 mg/kg or more
  • the GABA content in the drink is 800mg/kg
  • one panel member evaluated that the taste is unique and different from fruity sourness, so the upper limit for the GABA content should be around 800mg/kg. It was suggested that this is preferable.
  • This mixture is filled into 190ml cans, the air in the headspace is replaced with nitrogen gas, the cans are immediately sealed, heat sterilized at 115°C for 20 minutes, and cooled to produce packaged black coffee beverages. did.
  • a canned black coffee beverage was produced in the same manner except that GABA was not added.
  • the concentration of furfuryl acetate in these black coffee drinks was 75 ⁇ g/kg, the Brix value was 1.4%, and the pH was 6.8.
  • the resulting canned black coffee beverage was subjected to sensory evaluation in the same manner as in Experimental Example 4. The results are shown in Table 6. All the panel members evaluated that the refreshing sour taste unique to coffee was enhanced by including GABA.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Tea And Coffee (AREA)

Abstract

L'invention concerne une boisson au café noir d'une gamme neutre ayant un arôme amélioré. Une boisson au café noir ayant un pH de 6,0 à 7,5 est amenée à contenir de l'acide γ-aminobutyrique en une concentration de 60 à 800 mg/kg.
PCT/JP2023/008262 2022-04-28 2023-03-06 BOISSON AU CAFÉ NOIR CONTENANT DE L'ACIDE γ-AMINOBUTYRIQUE WO2023210168A1 (fr)

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JP2022-074576 2022-04-28
JP2022074576A JP7329100B1 (ja) 2022-04-28 2022-04-28 γ－アミノ酪酸を含有するブラックコーヒー飲料

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010187554A (ja) * 2009-02-16 2010-09-02 Oriza Yuka Kk γ−アミノ酪酸含有コーヒー豆抽出物
CN106900954A (zh) * 2017-02-25 2017-06-30 康田 一种含有γ‑氨基丁酸的咖啡
JP2022069272A (ja) * 2020-10-23 2022-05-11 サントリーホールディングス株式会社 γ－アミノ酪酸を含有する容器詰コーヒー飲料

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010187554A (ja) * 2009-02-16 2010-09-02 Oriza Yuka Kk γ−アミノ酪酸含有コーヒー豆抽出物
CN106900954A (zh) * 2017-02-25 2017-06-30 康田 一种含有γ‑氨基丁酸的咖啡
JP2022069272A (ja) * 2020-10-23 2022-05-11 サントリーホールディングス株式会社 γ－アミノ酪酸を含有する容器詰コーヒー飲料

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YAMATSU, A. ET AL.: "The beneficial effects of coffee on stress and fatigue can be enhanced by the addition of GABA -A randomized, double-blind, placebo controlled, crossover-designed study-", JAPANESE PHARMACOLOGY AND THERAPEUTICS, RAIFU SAIENSU SHUPPAN, TOKYO., JP, vol. 43, no. 4, 1 January 2015 (2015-01-01), JP , pages 515 - 519, XP009550023, ISSN: 0386-3603 *

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JP7329100B1 (ja) 2023-08-17
JP2023163587A (ja) 2023-11-10
JP2023164428A (ja) 2023-11-10

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