WO2022063296A1 - Additif pour aliment pour animaux pour améliorer la capacité d'obtention d'oxygène de volailles et son utilisation - Google Patents

Additif pour aliment pour animaux pour améliorer la capacité d'obtention d'oxygène de volailles et son utilisation Download PDF

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Publication number
WO2022063296A1
WO2022063296A1 PCT/CN2021/120945 CN2021120945W WO2022063296A1 WO 2022063296 A1 WO2022063296 A1 WO 2022063296A1 CN 2021120945 W CN2021120945 W CN 2021120945W WO 2022063296 A1 WO2022063296 A1 WO 2022063296A1
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WIPO (PCT)
Prior art keywords
feed
poultry
feed additive
activity
parts
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PCT/CN2021/120945
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English (en)
Chinese (zh)
Inventor
余璐璐
张广民
宋全芳
王海燕
李阳
高继磊
蔡辉益
李爽
Original Assignee
天津博菲德科技有限公司
北京挑战农业科技有限公司
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Publication of WO2022063296A1 publication Critical patent/WO2022063296A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof

Definitions

  • the invention relates to the technical field of feed additives, in particular to a feed additive which can be added in poultry raising and can effectively improve oxygen supply capacity and improve the survival rate and production performance of poultry and its application.
  • 5-aminolevulinic acid (-ALA), molecular formula C 5 H 9 NO 3 , is a ⁇ -amino acid with an amino group and a carboxyl group on both sides.
  • 5-ALA widely exists in animal, plant and microbial cells, and is non-toxic to humans and animals. It is easily degraded in the environment and has no residue. Currently, it is gradually popularized and applied in the domestic feed industry.
  • 5-ALA is closely related to the regulation of heme synthesis in the body, and heme is the main stable structure on the hemoglobin molecule and participates in the transport of oxygen and carbon dioxide. Therefore, adding 5-ALA to livestock and poultry feeds It can effectively improve the blood oxygen content of livestock and poultry, activate cell function, and accelerate intracellular redox reaction, thereby promoting vigorous metabolism of the body and improving feed conversion rate; at the same time, some studies have found that 5-ALA can significantly promote the body's hematopoietic function and increase the red blood cells in the blood. 5-ALA in livestock and poultry feeds can regulate the immune system, increase the number of immunoglobulins and white blood cells in the body, and enhance resistance to pathogenic microorganisms ability.
  • Feed enzyme preparation and Enterococcus faecalis are a new type of biological preparation products, which are non-toxic and non-residual, and have significant application effects in regulating the intestinal health of livestock and poultry and improving production performance.
  • Feed enzymes and Enterococcus faecalis can regulate the body's immune system by reducing the anti-nutritional factors in the feed, reducing the viscosity of the chyme in the intestinal tract, improving the micro-ecological balance of the intestinal flora, and improving the disease resistance of livestock and poultry. metabolic state, thereby improving the turnover efficiency of nutrients.
  • Enterococcus faecalis is a facultative anaerobic gram-positive lactic acid bacterium with a spherical or chain shape. Enterococcus faecalis can form a biofilm in the animal intestine and attach to the animal intestinal mucosa, and develop, grow and develop. Reproduction can form a barrier of lactic acid bacteria to resist the side effects of foreign bacteria, viruses and mycotoxins.
  • the commonly used feed additives such as the separate addition of feed compound enzymes and Enterococcus faecalis to the feed, cannot significantly improve the oxygen supply capacity of livestock and poultry, and the effects in terms of production performance are very limited.
  • the purpose of the present invention is to aim at the problems of limited growth performance and high lethality of poultry due to the problem of oxygen supply in the prior art, and to provide a feed additive for improving the ability of poultry to obtain oxygen, the active ingredient of which is 5-amino Levulinic acid has a significant effect on improving the efficiency of oxygen utilization in poultry.
  • a large number of animal tests have been screened and verified that 5-ALA, feed compound enzymes and Enterococcus faecalis have a synergistic effect, and their combined use can significantly improve the blood oxygen supply and oxygen supply of poultry, and improve the survival rate and production performance.
  • the present invention provides a feed additive for improving the oxygen acquisition capacity of poultry, the feed additive comprises active ingredients, and the active ingredients are 5-aminolevulinic acid, 5-aminolevulinic acid derivatives or 5-aminolevulinic acid at least one of levulinate.
  • the 5-aminolevulinic acid derivative can be 5-aminolevulinic acid ester, N-acyl-5-aminolevulinic acid, N-acyl-5-aminolevulinic acid ester, etc.;
  • Described 5-aminolevulinic acid salt can be 5-aminolevulinic acid hydrochloride, 5-aminolevulinic acid hydrobromide, 5-aminolevulinic acid phosphate, 5-aminolevulinic acid nitrate, 5-aminolevulinic acid sulfate, etc.
  • the feed additive may be a composite additive in the following mass ratio, the feed additive comprising 2-10 parts by weight of active ingredients, 10-45 parts by weight of composite enzymes for feed, 10 parts by weight of Enterococcus faecalis - parts by weight and carrier 0-75 parts by weight.
  • the feed additive comprises 2-10 parts by weight of active ingredients, 10-45 parts by weight of complex enzymes for feed, and 10-45 parts by weight of Enterococcus faecalis.
  • the feed additive comprises 5 parts by weight of active ingredients, 30 parts by weight of complex enzymes for feed, 30 parts by weight of Enterococcus faecalis and 35 parts by weight of carrier.
  • the multi-enzyme for feed comprises ⁇ -mannanase, xylanase, ⁇ -glucanase, pectinase and cellulase
  • ⁇ -mannanase activity is 2000-6000U/g
  • xylanase activity is 8000-20000U/g
  • ⁇ -glucanase activity is 400-1000U/g
  • pectinase activity is 200-1000U/g
  • cellulase activity of 300-600U/g cellulase activity of 300-600U/g.
  • the forage complex enzyme comprises ⁇ -mannanase activity of 5000U/g, xylanase activity of 15000U/g, ⁇ -glucanase activity of 800U/g,
  • the pectinase activity was 600 U/g and the cellulase activity was 600 U/g.
  • the viable count of Enterococcus faecalis is 5 ⁇ 10 9 to 2 ⁇ 10 10 cfu/g.
  • the viable count of Enterococcus faecalis is 1 ⁇ 10 10 cfu/g.
  • the carrier is selected from corn starch, wheat starch, tapioca starch, potato starch, beta-cyclodextrin, maltodextrin, modified starch.
  • the present invention provides a feed for improving the oxygen acquisition capacity of poultry, the feed comprising the above-mentioned feed additive.
  • the invention provides a poultry breeding method, which includes the step of using the feed additive for poultry feeding.
  • the present invention provides a poultry breeding method, comprising the steps of using the feed to feed the poultry.
  • the following examples facilitate a better understanding of the present invention, but do not limit the present invention.
  • the experimental methods in the following examples are conventional methods unless otherwise specified.
  • the test materials used in the following examples were purchased from conventional biochemical reagent stores unless otherwise specified.
  • the quantitative tests in the following examples are all set to repeat the experiments three times, and the results are averaged.
  • the effective active ingredient of 5-ALA in the embodiment of the present invention is ⁇ 99%, purchased from Beijing Challenge Biotechnology Co., Ltd., and the trade name is Wuleyi ; From Beijing Challenge Biotechnology Co., Ltd., the trade name is You C-1000; the compound feed enzyme preparation is ⁇ -mannanase activity of 5000U/g, xylanase activity of 15000U/g, ⁇ -glucanase activity The activity was 800U/g, the pectinase activity was 600U/g, and the cellulase activity was 600U/g, which were purchased from Beijing Challenge Biotechnology Co., Ltd. under the trade name of Teji NSPase.
  • the control group was fed with the basal diet + 500g/t corn starch; the experimental group was fed with 25g/t5-ALA + 475g/t corn starch in the basal diet; the experimental group was fed with 150g/t in the basal diet Enzyme preparation + 150g/t Enterococcus faecalis + 200g/t corn starch; experimental group 3 was supplemented with 25g/t 5-ALA + 150g/t feed enzyme preparation + 150g/t Enterococcus faecalis + 175g in the basal diet /t corn starch, the experimental design is shown in Table 1:
  • the feeding test was carried out at the Zunhua Animal Test Base of Beijing Challenge Group.
  • the test process was routine feeding, free access to food and water, and immunization and medication were operated in accordance with the "Immunization Procedures" and "Medication Guidelines” of the chicken farm.
  • the feeding experiment was performed on an empty stomach in the morning at the beginning and the end of the experiment, and the daily feed intake was recorded during the experiment.
  • 6 broilers that had been fasted for 12 hours were randomly selected from each group, half male and female, and blood was collected from the heart in an EDTA-K 2 anticoagulation tube, and the number of red blood cells (RBC) and the concentration of hemoglobin (HGB) were detected by an automatic blood analyzer. and hematocrit PCV.
  • the control group was fed the basal diet; the experimental group was supplemented with 15g/t 5-ALA+150g/t feed enzyme preparation+150g/t Enterococcus faecalis in the basal diet; the experimental group was supplemented with the basal diet 25g/t 5-ALA+150g/t feed enzyme preparation+150g/t Enterococcus faecalis; the three groups in the experiment were basal diet supplemented with 35g/t 5-ALA+150g/t feed enzyme preparation+150g/t Enterococcus faecalis, the experimental design is shown in Table 4:
  • the 5-ALA described in this test is the effective active ingredient ⁇ 99%; the enzymatic activity of the feed enzyme preparation is ⁇ -mannanase activity of 5000U/g, xylanase activity of 15000U/g, ⁇ -glucanase activity
  • the carbohydrase activity is 800U/g
  • the pectinase activity is 600U/g
  • the cellulase activity is 600U/g
  • the viable count of the Enterococcus faecalis for feeding is 1*10 10 cfu/g.
  • the feeding test was carried out in the Zunhua Animal Test Base of Beijing Challenge Group.
  • the test process was conventional feeding, free access to food and water, and immunization and medication were operated in accordance with the "Immunization Procedures" and "Medication Guidelines” of the chicken farm.
  • the feeding test was weighed on an empty stomach in the morning at the beginning and end of the test, and the daily feed intake was recorded during the test. After the test, the average feed intake, daily gain and feed-to-meat ratio of the broilers were calculated. The feeding test was carried out on 42d. At age, 6 broilers that had been fasted for 12 hours were randomly selected from each group, half male and female, and blood was collected from the heart in an EDTA-K 2 anticoagulant tube to detect the number of red blood cells RBC, hemoglobin concentration HGB and red blood cells by an automatic blood analyzer. Accumulated PCV.
  • each test group can significantly increase the number of red blood cells RBC of broiler chickens on 42 days (P ⁇ 0.05).
  • RBC red blood cells
  • HGB hemoglobin content
  • hematocrit PCV of broiler chickens on 42 days were significantly different from those of the control group (P ⁇ 0.05); Significantly improve the oxygen supply capacity of broilers.
  • a total of 1380 healthy Korean quails aged 36 weeks were selected and randomly divided into 4 groups with 6 replicates (cage) in each group.
  • the feeding amount of each repeated (cage) quail in one test group is 60/cage, and the feeding amount of quail in two groups, three groups, four groups, five groups, six groups and seven groups is 65/cage.
  • Cages, experimental groups 1 and 2 were fed basal diet, experimental group 3 was supplemented with 25 g/t 5-ALA in the basal diet, experimental group 4 was supplemented with 35 g/t 5-ALA in the basal diet, and experimental group 5 was fed with basal diet.
  • the six experimental groups were to add 25g/t 5-ALA + 150g/t feed enzyme preparation + 150g/t to the basal diet Enterococcus faecalis; the test quails were housed in stacked cages, and the area of the quail cages was 0.7345m 2 .
  • the pre-trial period is 3d, and the trial period is 28d.
  • the 5-ALA described in this test is the effective active ingredient ⁇ 99%; the enzyme activity of the feed enzyme preparation is 5000U/g of ⁇ -mannanase activity, 15000U/g of xylanase activity, and ⁇ -glucan.
  • the carbohydrase activity is 800U/g
  • the pectinase activity is 600U/g
  • the cellulase activity is 600U/g;
  • the viable count of the Enterococcus faecalis for feeding is 1*10 10 cfu/g.
  • the feeding test was conducted at the Zunhua Animal Test Base of Beijing Challenge Group.
  • the test quails were fed and drank ad libitum according to the conventional feeding method of the farm.
  • the quails in each group were fed the same diet, and the diet was self-made conventional quail diet, which could meet the nutritional needs of the quails in the experimental period.
  • Quail eggs were collected and weighed at 08:00 every day after the start of the experiment, the total number and total egg weight of each replicate quail eggs were recorded, and the egg production rate and average egg weight of each replicate quail were calculated.
  • the application of the additive of the present invention in poultry can significantly improve the blood oxygen supply capacity of the poultry, reduce the death rate of the poultry, improve the survival rate (especially reduce the incidence of ascites in broilers), and can improve the production performance of the poultry.
  • the product has strong economic application benefit and market promotion value.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Birds (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

La présente invention concerne un additif pour aliment pour animaux pour améliorer la capacité d'obtention d'oxygène de volailles, ledit additif comprenant au moins l'un des composants actifs parmi l'acide 5-aminolévulinique, un dérivé d'acide 5-aminolévulinique ou un 5-aminolévulinate. L'additif peut en outre être un additif ajouté en parties en masse, qui comprend 2 à 10 parties d'un composant actif, 10 à 45 parties d'un composé enzymatique pour utilisation dans l'alimentation animale, 10 à 45 parties d'Enterococcus faecalis et 0 à 75 parties d'un véhicule. L'invention concerne en outre l'utilisation de l'additif pour aliment pour animaux dans l'élevage de volailles.
PCT/CN2021/120945 2020-09-28 2021-09-27 Additif pour aliment pour animaux pour améliorer la capacité d'obtention d'oxygène de volailles et son utilisation WO2022063296A1 (fr)

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CN202011037608.3A CN112075546A (zh) 2020-09-28 2020-09-28 一种用于提高家禽获氧能力的饲料添加剂及其应用
CN202011037608.3 2020-09-28

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CN112075546A (zh) * 2020-09-28 2020-12-15 北京挑战农业科技有限公司 一种用于提高家禽获氧能力的饲料添加剂及其应用
CN112931713A (zh) * 2021-03-23 2021-06-11 山东深海生物科技股份有限公司 一种改善母仔猪铁营养状况的促进剂及其应用
CN112890006B (zh) * 2021-05-07 2021-08-13 北京大北农动物保健科技有限责任公司 一种造血营养饲料添加剂及其制备方法和其用途
CN113170840A (zh) * 2021-05-20 2021-07-27 河南邑鸿善成生物技术有限公司 含5-氨基乙酰丙酸和甘氨酸铁的饲料添加剂及制备方法与应用
CN114158657A (zh) * 2021-12-20 2022-03-11 北京挑战农业科技有限公司 一种提高家禽抗氧化能力和免疫力的功能性氨基酸及其应用
CN115024434B (zh) * 2022-08-11 2022-10-28 北京挑战生物技术有限公司 一种延长蛋鸡产蛋周期的饲料添加剂及其应用

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