WO2020085301A1 - ろ過用フィルター、フィルター付容器、及び細胞懸濁液中の異物除去方法 - Google Patents
ろ過用フィルター、フィルター付容器、及び細胞懸濁液中の異物除去方法 Download PDFInfo
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- WO2020085301A1 WO2020085301A1 PCT/JP2019/041306 JP2019041306W WO2020085301A1 WO 2020085301 A1 WO2020085301 A1 WO 2020085301A1 JP 2019041306 W JP2019041306 W JP 2019041306W WO 2020085301 A1 WO2020085301 A1 WO 2020085301A1
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- filter
- welding frame
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- density polyethylene
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/08—Filter cloth, i.e. woven, knitted or interlaced material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/08—Filter cloth, i.e. woven, knitted or interlaced material
- B01D39/083—Filter cloth, i.e. woven, knitted or interlaced material of organic material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/08—Filter cloth, i.e. woven, knitted or interlaced material
- B01D39/086—Filter cloth, i.e. woven, knitted or interlaced material of inorganic material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
- B01D39/1607—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous
- B01D39/1623—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous of synthetic origin
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/20—Other self-supporting filtering material ; Other filtering material of inorganic material, e.g. asbestos paper, metallic filtering material of non-woven wires
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/20—Other self-supporting filtering material ; Other filtering material of inorganic material, e.g. asbestos paper, metallic filtering material of non-woven wires
- B01D39/2027—Metallic material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/20—Other self-supporting filtering material ; Other filtering material of inorganic material, e.g. asbestos paper, metallic filtering material of non-woven wires
- B01D39/2055—Carbonaceous material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07B—SEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
- B07B1/00—Sieving, screening, sifting, or sorting solid materials using networks, gratings, grids, or the like
- B07B1/46—Constructional details of screens in general; Cleaning or heating of screens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
- C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/12—Special parameters characterising the filtering material
- B01D2239/1216—Pore size
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
- B01D2239/12—Special parameters characterising the filtering material
- B01D2239/1233—Fibre diameter
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Definitions
- the technology of the present disclosure relates to a filter for filtration, a container with a filter, and a method for removing foreign matter contained in a cell suspension.
- the cell suspension after culturing contains medium, serum, carrier for culture, waste products and debris derived from cells, and these need to be separated and removed. Furthermore, it is necessary to wash and concentrate the cells even after removing them, and a centrifugation method is known as a method for performing these.
- a culture container for culturing cells a medium storage container for storing a medium and the like, a cell injection container for injecting cells, and a cell suspension after culturing.
- a cell culture kit has been proposed in which a closed cell environment is constructed by connecting a cell collection container for collecting a suspension with a conduit. According to such a cell culture kit, cell injection, medium addition, sampling, and collection can be performed while maintaining a closed system in the kit.
- the culture vessel in recovering the cultured cells, is left stationary to allow the cells in the cell culture solution to settle, and then the supernatant of the cell culture solution is discharged to determine the volume of the solution.
- An example is shown in which the cell culture solution that has been reduced and then concentrated is transferred from the culture container to the cell recovery container.
- the cells in the cell suspension must be allowed to settle by leaving the culture container stationary before discharging the supernatant of the cell culture solution. It takes time to settle. Furthermore, even if the cells in the cell culture medium are allowed to settle for a sufficient period of time, the cells may be mixed into the supernatant due to the discharging operation, and the cells may be discharged together with the supernatant.
- WO 2014/007382 describes that sufficient welding strength was obtained by laser welding in which a molten resin layer is welded to a resin layer facing each other by intruding the resin layer into a hole of a metal filter. There is. However, there is a problem in that the metal filter is poor in flexibility as a material used for closed cell treatment.
- JP 2006-231875 A discloses a vehicle fluid filter device in which a polyester resin fiber (nonwoven fabric) is used as a filter and is sandwiched between case constituent members.
- one of the case constituent members is a resin such as nylon 66 or nylon 6 which is not colored with a pigment or the like, and the other is a resin such as nylon 66 or nylon 6 which is colored with a pigment or the like. Is described.
- An object of the technology of the present disclosure is to filter a filter fixed between two polymer films with high welding strength, a filter-equipped container in which the filter is fused so as to partition the inside, and a container with a filter. It is intended to provide a method for removing foreign matter contained in a cell suspension by using.
- the inventors of the technology of the present disclosure produce a filter for filtration in which a filter is fixed between two polymer films, and a material for the polymer film that can obtain high welding strength between the polymer film and the filter. And the film thickness thereof and the porosity of the filter were found, and the technique of the present disclosure was completed based on this finding.
- the technology of the present disclosure is formed of, for example, a flexible polymer film containing a polymer and having a thickness of 120 ⁇ m or more, and formed in a frame shape having a first passage hole penetrating in the thickness direction on the inside.
- a second welding frame formed of a first welding frame and a flexible polymer film containing a polymer and having a thickness of 120 ⁇ m or more, and having a frame shape having a second through hole penetrating in the thickness direction inside
- High density polyethylene with a melting point of 120 ° C-140 ° C Len High Density Polyethylene
- LLDPE Linear Low Density Polyethylene
- a filter for filtration which is composed of a polymer containing a mixture.
- the "open area ratio” refers to the ratio of the area of the hole to the total area.
- the technology of the present disclosure includes a filter-equipped container in which such a filter for filtration is welded to the inside of a container made of a polymer, and thereby the inside of the container is partitioned.
- one embodiment of the technique of the present disclosure is to inject a cell suspension containing or possibly containing a foreign substance into one compartment inside the container in such a filter-equipped container, and filter the cell suspension.
- the present invention relates to a method of removing a foreign substance contained in a cell suspension by collecting the filtrate containing cells from the other compartment by passing through the column.
- the filter has pores that communicate with each other in the thickness direction, and the pores have a pore diameter that allows cells to pass through, but does not allow foreign matter of a certain size or more to pass through. Therefore, such foreign matter can be removed from the cell suspension by passing through the filtration filter.
- one embodiment of the technique of the present disclosure is to inject a cell suspension into one of the compartments inside the container in such a filter-equipped container, and pass the cell suspension through a filter for filtration to obtain a filtrate containing cells. Then, a solution for cell suspension was injected into the same compartment to resuspend the cells remaining in the same compartment, and the resuspended cell suspension was passed through a filtration filter to remove cells.
- the present invention relates to a method for removing foreign matter contained in a cell suspension, which comprises a step of collecting a filtrate containing
- a portion of the filter that is not sandwiched between the first welding frame and the second welding frame, that is, a portion corresponding to the first passage hole and the second passage hole is the filter.
- the filter and the first welding frame and the second welding frame are welded with high welding strength, the welding portion is unlikely to peel off, and there is little risk of fluid leaking from the welding portion.
- FIG. 2 is a sectional view taken along the line AA in FIG. 1.
- the material between the first welding frame 10 and the second welding frame 12 is higher than the material of the first welding frame 10 and the second welding frame 12.
- the filter 16 is a filtration filter 16 made of a material having a melting point and having a portion that is not sandwiched between the first welding frame 10 and the second welding frame 12 and fixed by welding.
- the material of the filter 14 is polyolefin such as polypropylene or polyethylene, polyester, polyvinyl chloride, polyvinyl alcohol, vinylidene chloride, acrylic polymer such as polymethylmethacrylate or polyacrylonitrile, polyamide such as nylon, polystyrene, polyurethane, polyimide, aramid, It is preferable to contain at least one selected from the group consisting of polyether ether ketone, polysulfone, rayon, cellulose, chitin, chitosan, cotton, hemp, glass, carbon fiber, and metal.
- polyester examples include polyethylene terephthalate, polybutylene terephthalate, and polymethylene terephthalate.
- polyamides examples include 6,6-nylon, 6-nylon and 12-nylon.
- the material of the filter 14 may be a porous body shape having a communication hole structure having a plurality of holes 14A, an aggregate of fibers, a non-woven fabric, a woven fabric, a knitted fabric or the like, but a woven fabric or a knitted fabric is preferable.
- the pore diameter of the pores 14A of the filter 14 is preferably 5 ⁇ m or more and 200 ⁇ m or less, which is a diameter necessary for capturing contaminants such as culture carrier residues other than cells. If the hole diameter of the hole portion 14A is smaller than 5 ⁇ m, the filter 14 may be clogged, and the efficiency of removing contaminants may decrease. On the other hand, if the pore diameter of the pores 14A is larger than 200 ⁇ m, it will be difficult to capture impurities and target cells.
- the pore diameter of the pores 14A is preferably 10 ⁇ m to 200 ⁇ m in view of the removal efficiency of contaminants such as a relatively large culture carrier residue and the ability to trap target cells.
- the porosity of the filter 14 is 10% or more and 80% or less in terms of welding strength. If the porosity is lower than 10%, the melted polymer film is less likely to be entangled with the filter 14 when it is welded to the polymer films forming the first welding frame 10 and the second welding frame 12, and problems such as delamination may occur. It is easy to cause. On the other hand, if the porosity of the filter 14 is larger than 80%, the strength of the filter 14 may decrease, and cutting, cracking, and cracking may occur, and also as the filtering filter 16, the mechanical strength may decrease. There is.
- the porosity of the filter 14 is preferably 10% or more and 70% or less, more preferably 10% or more and 50% or less.
- the material of the filter 14 has a higher melting point than the material of the first welding frame 10 and the second welding frame 12.
- the material of the filter 14 preferably has a higher melting point in the range of 80 ° C. or higher and 180 ° C. or lower, compared to the melting points of the materials of the first welding frame 10 and the second welding frame 12, and is 100 ° C. or higher.
- Those having a high melting point in the range of 160 ° C. or lower are more preferable, and those having a high melting point in the range of 110 ° C. or higher and 150 ° C. or lower are further preferable.
- the material of the first welding frame 10 and the second welding frame 12 is high density polyethylene having a melting point of 120 ° C.
- the melting point of the material of the filter 14 is preferably 200 ° C. or higher and 320 C. or less, more preferably 220.degree. C. or more and 300.degree. C. or less, still more preferably 230.degree. C. or more and 290.degree. C. or less.
- the melting point of the material of the filter 14 is preferably 185 ° C or more. It is 305 ° C. or less, more preferably 205 ° C. or more and 285 ° C. or less, still more preferably 215 ° C. or more and 275 ° C. or less.
- the thickness of the filter 14 is preferably 50 ⁇ m or more and 200 ⁇ m or less from the viewpoint of the strength of the filter 14 after welding. If the film thickness of the filter 14 is smaller than 50 ⁇ m, the strength of the filter 14 may decrease, and cutting, cracking, or cracking may occur, and the mechanical strength of the filtering filter 16 may decrease.
- the film thickness of the filter 14 is larger than 200 ⁇ m, when the polymer films forming the first welding frame 10 and the second welding frame 12 are melted, the pores of the filter 14 in the region near the surface of the filter 14 are formed. The melted polymer film material permeates only into 14A, and the first welding frame 10 and the second welding frame 12 facing each other are less likely to be welded, so that the welding strength may be insufficient.
- the materials of the first welding frame 10 and the second welding frame 12 are polypropylene, polyethylene, high density polyethylene, low density polyethylene, linear low density polyethylene, polyolefin such as ethylene-propylene copolymer, polyester, polyvinyl chloride, polyvinyl. Alcohol, vinylidene chloride, polystyrene, acrylic polymer such as polymethylmethacrylate or polyacrylonitrile, polyamide, polyurethane, polyimide, aramid, polyetheretherketone, polysulfone, and a mixture of one or more selected from the group consisting of carbon. Preferably there is.
- polypropylene, polyethylene, high density polyethylene, low density polyethylene, polyolefin polyester such as linear low density polyethylene, polyamide, and a mixture of one or more selected from the group consisting of polyvinyl alcohol are more preferable, and high density polyethylene or Most preferably it is a linear low density polyethylene.
- the melting point is preferably 120 ° C to 140 ° C, and when it is linear low density polyethylene, the melting point is preferably 105 ° C to 125 ° C.
- the density of linear low-density polyethylene having a temperature of from 0 ° C to 125 ° C is approximately 912 kg / m 3 to 930 kg / m 3 .
- the melting point of the above material is obtained as the temperature of the endothermic peak position in the measurement using a differential scanning calorimeter (DSC: Differential Scanning Calorimetry), and the density of the above material is JIS K 0061 It is obtained by the in-liquid weighing method described in “Determination of density and specific gravity of chemical products” and JIS Z8807 “Determination of density and specific gravity of solids”.
- both the first welding frame 10 and the second welding frame 12 have a frame shape having a first passage hole 10A or a second passage hole 12A penetrating in the thickness direction inside thereof. And, and i) The first passage hole 10A in the frame of the first welding frame 10. ii) A portion of the filter 14 that is not in contact with the frame portions 10B and 12B of the first welding frame 10 and the second welding frame 12, respectively. iii) The second passage hole 12A in the frame of the second welding frame 12 It is preferable that the first welding frame 10, the filter 14, and the second welding frame 12 are welded in such a manner that the fluid in the object to be filtered can move in the order of, or vice versa.
- first welding frame 10 and the second welding frame 12 are both frame-shaped, they may have the same shape or different shapes, but at the punching portion of the first welding frame 10, It is preferable that a certain first passage hole 10A and the second passage hole 12A that is a punched-out portion of the second welding frame 12 have the same shape, and among them, it is preferable that they have a rectangular shape (including a square shape). It is preferable that both the first welding frame 10 and the second welding frame 12 have similar shapes to the outer shapes of the first welding frame 10 and the second welding frame 12 (in this case, the widths of the frame portions 10B and 12B are substantially uniform). Will be).
- first passage hole 10A of the first welding frame 10 and the second passage hole 12A of the second welding frame 12 have the same rectangular shape, and the outer shape of the first welding frame 10 and the second welding frame 12 are the same. It is preferable that each of the outer shapes is a rectangular shape similar to that, and most preferably, the outer shapes of the first welding frame 10 and the second welding frame 12 are the same rectangular shape.
- the function of the filter 14 is exerted by the portion not sandwiched between the first welding frame 10 and the second welding frame 12, that is, the first welding frame 10 It is a portion corresponding to the second passage hole 12A of the passage hole 10A and the second welding frame 12, but in the filtration filter 16 of the technique of the present disclosure, both the first welding frame 10 and the second welding frame 12 are frame-shaped.
- the fluid in the filtration target is, as described above, the first passage hole 10A in the frame of the first welding frame 10, the first passage hole 10A of the first welding frame 10 in the filter 14, and the second welding frame.
- the first welding frame 10 and the second welding frame 12 are moved while the portion corresponding to the second passage hole 12A of 12 and the second passing hole 12A in the frame of the second welding frame 12 are moved in this order or vice versa. In the part where is welded, Movement of the fluid is prevented that.
- the filter 14 in the filtering filter 16 of the technology of the present disclosure exerts its function.
- the outer shape of the filter 14 is the same as that of the first welding frame 10 and the second welding frame 12 in the entire circumference of the first passage hole 10A of the first welding frame 10 and the second passage hole 12A of the second welding frame 12. It must be capable of forming an overlap.
- the shape of the filter 14 used in the technique of the present disclosure is preferably a rectangular shape (including a square shape), and in that case, the first welding frame 10 and the second welding frame 12 are both of the shape of the filter 14 having such a shape. It is preferable that they have the same shape in which they overlap at the peripheral portion.
- the film thicknesses of the first welding frame 10 and the second welding frame 12 are both 120 ⁇ m or more in order to obtain high welding strength with the filter 14.
- the upper limit of the film thickness of the first welding frame 10 and the second welding frame 12 is not particularly limited from the viewpoint of welding strength with the filter 14, but is preferably 500 ⁇ m or less from the viewpoint of providing flexibility.
- the film thickness of the first welding frame 10 and the second welding frame 12 is more preferably 200 ⁇ m or more and 400 ⁇ m or less, and most preferably 200 ⁇ m or more and 300 ⁇ m or less.
- the welding strength of the welded portion 34 is preferably 20 N / 15 mm or more, more preferably 23 N / 15 mm or more, and further preferably, when measured by the method described in Example B (evaluation method of welding strength). It is 30 N / 15 mm or more.
- the welding strength of the welding portion 34 is 20 N / 15 mm to 80 N / 15 mm, 23 N / 15 mm to 60 N / 15 mm, or 30 N / 15 mm to 60 N / 15 mm.
- the standard value of JIS Z0238 is 23 N / 15 mm, but also in the filter 16 for filtration of the technique of the present disclosure, this numerical value is a standard of the welding strength of the welding portion 34 to be achieved. However, even if the welding strength of the welded portion 34 falls below this value, it does not mean that the welded portion 34 cannot be used immediately.
- the filter 16 for filtration in the technique of the present disclosure sandwiches the filter 14 between the first welding frame 10 and the second welding frame 12, and sets them above the melting point of the first welding frame 10 or the second welding frame 12.
- the molten polymer of the first welding frame 10 and the second welding frame 12 is caused to enter the hole portion 14A of the unmelted filter 14, and then, for example, heat radiation is performed.
- solidifying the polymer by means of the above, it means a laminated body including three layers in which each of the first welding frame 10 and the second welding frame 12 and the filter 14 are fixed via the welding portion 34.
- the polymer melted from both sides of the filter 14 is connected by penetrating through the holes 14A of the filter 14 which are not melted, and the first welding frame 10 and the second welding frame 12 facing each other are fixed to each other. Those that have been described are preferable.
- the filter 16 for filtration of the technique of the present disclosure may be welded to another member via at least one of the first welding frame 10 and the second welding frame 12.
- at least one of the first welding frame 10 and the second welding frame 12 in the technique of the present disclosure functions as a medium for realizing welding with another member.
- one embodiment of the technology of the present disclosure is that a filter 16 for filtration is welded inside a container 20 including two polymer sheets (20A, 20B), thereby the inside of the container 20.
- a container 22 with a filter which is partitioned.
- the filter-equipped container in the technique of the present disclosure will be described in detail by taking the filter-equipped container 22 shown in FIG. 4 as an example.
- partitioned means that, as shown in FIG. 4, from the compartment S1 which is a space formed by one surface of the filter 16 for filtration and the inside of the container 20, to the other of the filter 16 for filtration.
- the flow path for the fluid to move to the compartment S2, which is a space formed by the surface and the inside of the container 20, does not occur except for the filter 14 of the filter 16 for filtration, and the filter 16 for filtration causes the flow path of the container 20 It means that the inside is partitioned.
- the container 22 with a filter according to the technology of the present disclosure functions as a filter.
- the container 20 in the technique of the present disclosure includes two polymer sheets (20A, 20B).
- the material of these polymer sheets is a flexible plastic, and specific examples thereof include the same as those described above for the second welding frame 12 in the technique of the present disclosure, and the preferred ones also have the same physical properties (especially melting point). Among them, those having compatibility with the material of the first welding frame 10 and the material of the second welding frame 12 are particularly preferable. This is because, when fixing the filter 16 for filtration of the technique of the present disclosure to the inside of the container 20, a welding method can be used.
- the material of the first welding frame, the second welding frame, and the flexible plastic is high-density polyethylene having a melting point of 120 to 140 ° C.
- the material of the flexible plastic is the same as that of the high-density polyethylene.
- Density polyethylene can be preferably used.
- the material of the first welding frame and the second welding frame is linear low density polyethylene having a melting point of 105 ° C. to 125 ° C.
- Polyethylene can be preferably used.
- the shape of the container 20 preferably, one manufactured by welding two sheets of polymer sheets having the same shape, for example, a rectangular shape, facing each other and welding the peripheral edge portions thereof.
- the filter 16 for filtration is also made into a substantially similar shape which is slightly smaller than the rectangular shape of the container 20 (see FIG. 1), and the first welding frame 10 is welded to one inner surface of the container 20 at the first welding portion 24. It is preferable that the second welding frame 12 is welded to the other inner surface of the container 20 at the second welding portion 26.
- the polymer “sheet” forming the container 20 and the polymer “film” forming the first welding frame 10 and the second welding frame 12 are substantially the same in that the materials are flexible, In the present embodiment, in order to make the parts used easy to understand, they will be described separately as “sheet” and “film”.
- the first welding portion 24 is formed on one polymer sheet of the container 20 as viewed in the thickness direction of the first welding frame 10 (in this embodiment, one end of the container 20 in the longitudinal direction of the container 20).
- Part side is formed in a substantially U-shape that is open.
- the second welded portion 26 is opened to the other polymer sheet of the container 20 on the other side (the other end side in the longitudinal direction of the container 20 in the present embodiment) when viewed in the thickness direction of the second welded frame 12.
- the container 20 used in the technique of the present disclosure is provided with at least one of the injection port 30 and the pouring port 32. It is particularly preferable that one of the compartments in the container 20 partitioned by the filter 16 is provided with the injection port 30 and the other compartment is provided with the pouring port 32.
- the injection port 30 and the pouring port 32 may be plural in number, and for example, the pouring port 30 and the pouring port 32 may be provided in both of the compartments.
- the injection port has a passage that connects the inside and the outside of the container, and is used to inject the cell suspension into the container 20. There is no other passage through which the cell suspension can be injected into the container 20 of the filter-equipped container 22.
- the pouring port is for pouring out the cell suspension from the container 20.
- the container 20 of the filter-equipped container 22 There is no other passage through which the cell suspension can be poured into the container 20 of the filter-equipped container 22. That is, in the open portions of the first welded portion 24 and the second welded portion 26, the polymer sheet 20A and the polymer sheet 20B are welded to each other except where the injection port and the pouring port are attached. There is.
- the pouring port and the pouring port are welded or adhered to the polymer sheet 20A and the polymer sheet 20B, and the inside of the container 20 is hermetically sealed except for the passages of the ports.
- the container 22 with a filter may be one in which the inside of the container 20 is partitioned into a space of n + 1 by n (n is a natural number of 2 or more) filtration filters 16.
- n is a natural number of 2 or more
- some or all of the compartments inside the container 20 may be provided with at least one of the injection port 30 and the pouring port 32 that can be connected to the interior of each compartment.
- the technique of the present disclosure injects a cell suspension (not shown) into one compartment S1 inside the container 20 in the filter-equipped container 22 of the technique of the present disclosure, and from the other compartment S2. It is a method for removing foreign matter contained in a cell suspension, which comprises a step of collecting a cell suspension free of foreign matter.
- a cell suspension not shown
- foreign substances such as unnecessary culture carriers and cell aggregates remain.
- the cell suspension is injected into one compartment S1 inside the container 20 in the container 22 with the filter of the present disclosure, the cells are filtered by the filter 14, and the cells are resuspended.
- a method for removing foreign matter contained in the cell suspension which comprises the steps of injecting a liquid for treatment into the same section S1, resuspending the cells, and collecting the cell suspension from the same section S1.
- an operation of separating cell suspension components by a filter 14 is indispensable.
- the force for example, the weight of the cell suspension itself, pressurization of the compartment S1 inside the container 20 in which the cell suspension is injected, or decompression of the other compartment S2 can be used.
- a method for measuring the melting point, the density, and the film thickness of the polymer film used as the first polymer film forming the first welding frame 10 and the second polymer film forming the second welding frame 12 and the hole portion 14A are provided.
- the measuring method of the porosity, the pore diameter, and the fiber diameter of the filter used as the soot filter 14 is as follows.
- A-1) Measuring method of melting point (Tm) of polymer film The melting point of the polymer film was measured by DSC (TA Instruments, Q20). The DSC measurement conditions were a nitrogen atmosphere (50 ml / min), a measurement temperature range of 30 ° C. to 200 ° C., and a temperature rising rate of 10 ° C./min. As the melting point of the polymer film, the temperature at the peak position of the endothermic peak seen by melting in DSC was used.
- A-2 Method for measuring density of polymer film
- the density of the polymer film was measured by an in-liquid weighing method.
- a specific gravity measuring jig made by Mettler Toledo
- a balance made by Mettler Toledo, Blance XS105
- the polymer film was weighed in air, and then weighed in ethanol.
- the temperature of the liquid is measured, the density of ethanol is determined by the method described in the literature (Dweight E. Gray, American Institute of Physics Handbook, McGraw-Hill Book Company Inc., 1957), and the density of ethanol is determined according to the following formula. I calculated.
- ⁇ ⁇ A / (AB) ⁇ ⁇ ( ⁇ 0-d) + d
- ⁇ is the density of the sample
- A is the weight in the air
- B is the weight in the liquid
- ⁇ 0 is the density of the liquid
- d is the density of the air (0.002 g / cm 3 ).
- the outer circumference of the filter 14 was sandwiched between the first polymer film forming the first welding frame 10 and the second polymer film forming the second welding frame 12 over the entire circumference.
- the first welding frame 10 is heated by using an impulse sealer (SURE NL-102JW manufactured by Ishizaki Electric Co., Ltd.) set at 230 ° C. which is higher than the melting point of the polyethylene film and lower than the melting point of the PET filter 14.
- the filter 14 and the second welding frame 12 were welded together via the welding portion 34.
- the welding portion 34 was formed over the entire circumference of the portion of the filter 14 sandwiched between the first welding frame 10 and the second welding frame 12.
- a portion of the filter that is not sandwiched between the first welding frame 10 and the second welding frame 12, that is, a portion corresponding to the first passage hole 10A and the second passage hole 12A serves as a filter.
- the materials of the first polymer film forming the first welding frame 10, the second polymer film forming the second welding frame 12 and the filter 14 were changed variously, and the following Example 1 was used. -18 and the materials described in Comparative Examples 1-18 were prepared.
- Comparative Examples 19 to 20 the first polymer film and the second polymer film forming the second welding frame 12 are welded, which does not correspond to the filter 16 for filtration. The welding strength was evaluated.
- Ez-Test-Ez-SX tensile tester
- the standard value of JIS Z0238 is 23 N / 15 mm, but also in the filter 16 for filtration of the technique of the present disclosure, this numerical value is a standard of the welding strength of the welding portion 34 to be achieved.
- the welding strength of the welded portion 34 was 33.3 N / 15 mm.
- the welding strength of the welded portion 34 was 26.7 N / 15 mm.
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 NBC mesh
- T-380T pore diameter 28 ⁇ m, fiber diameter 35 ⁇ m, porosity 23%
- Tm 254 ° C., length 80 mm, width 15 mm
- the welding strength of the welded portion 34 was 13.9 N / 15 mm.
- the welding strength of the welded portion 34 was 13.9 N / 15 mm.
- the welding strength of the welded portion 34 was 14.9 N / 15 mm.
- LDPE low-density polyethylene films
- Tm 111 ° C., density 0.915 g / cm 3 , film thickness 500 ⁇ m, length 80 mm, width 15 mm.
- T-380T, pore diameter 28 ⁇ m, fiber diameter 35 ⁇ m, porosity 23%, Tm 254 ° C., length 80 mm, width 15 mm) were sandwiched and welded in this state to obtain a filter 16 for filtration.
- the welding strength of the welded portion 34 was 14.4 N / 15 mm.
- the welding strength of the welded portion 34 was 29.0 N / 15 mm.
- the welding strength of the welded portion 34 was 24.2 N / 15 mm.
- LLDPE linear low-density polyethylene films
- the welding strength of the welded portion 34 was 24.2 N / 15 mm.
- the welding strength of the welded portion 34 was 32.7 N / 15 mm.
- LLDPE linear low-density polyethylene films
- the welding strength of the welded portion 34 was 29.4 N / 15 mm.
- the welding strength of the welding portion 34 was 32.6 N / 15 mm.
- LLDPE linear low-density polyethylene films
- the welding strength of the welded portion 34 was 30.5 N / 15 mm.
- the welding strength of the welded portion 34 was 52.2 N / 15 mm.
- LLDPE linear low-density polyethylene films
- the welding strength of the welded portion 34 was 35.8 N / 15 mm.
- the welding strength of the welded portion 34 was 53.6 N / 15 mm.
- LLDPE linear low-density polyethylene films
- the welding strength of the welded portion 34 was 35.6 N / 15 mm.
- the welding strength of the welded portion 34 was 13.1 N / 15 mm.
- the welding strength of the welded portion 34 was 12.3 N / 15 mm.
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 made by Sefar
- pore diameter 6 ⁇ m, fiber diameter 34 ⁇ m, porosity 5%, Tm 257 ° C., length 80 mm, width 15 mm
- the welding strength of the welded portion 34 was 6.0 N / 15 mm.
- the welding strength of the welded portion 34 was 18.5 N / 15 mm.
- ⁇ Comparative Example 12> Two linear low-density polyethylene films (LLDPE, manufactured by Mitsui Chemicals Tohcello, HC # 100, Tm 124 ° C., density 0.922 g / cm 3 , film thickness 100 ⁇ m, length 80 mm, width 15 mm) were stacked and welded to form a film. A film having a thickness of 200 ⁇ m was prepared.
- LLDPE linear low-density polyethylene films
- the welding strength of the welded portion 34 was 17.1 N / 15 mm.
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 made by Sefar
- PET15 pore diameter 15 ⁇ m, fiber diameter 37 ⁇ m, porosity 9%
- Tm 257 ° C., length 80 mm, width 15 mm
- the welding strength of the welded portion 34 was 8.1 N / 15 mm.
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 made by Sefar
- Tm 257 ° C., a length of 80 mm, and a width of 15 mm
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 made by Sefar
- Tm 256 ° C., length 80 mm, width 15 mm
- the welding strength of the welding portion 34 was 13.8 N / 15 mm.
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 NBC mesh
- pore diameter 28 ⁇ m fiber diameter 35 ⁇ m, porosity 23%
- Tm 254 ° C., length 80 mm, width 15 mm
- the welding strength of the welded portion 34 was 13.9 N / 15 mm.
- LDPE low-density polyethylene film
- Tm 112 ° C., density 0.923 g / cm 3 , film thickness 200 ⁇ m, length 80 mm, width 15 mm
- PET filters 14 NBC mesh
- pore diameter 86 ⁇ m fiber diameter 55 ⁇ m, porosity 37%
- Tm 255 ° C., length 80 mm, width 15 mm
- the welding strength of the welded portion 34 was 21.6 N / 15 mm.
- the porosity of the filter 14 affects the welding strength. That is, when the porosity of the filter 14 is in the range of approximately 10% to 40%, the higher the porosity, the higher the welding strength with the film tends to be. However, when the film material is HDPE or LLDPE, the porosity is higher. The welding strength tended to be saturated when the ratio was about 40%. If the porosity of the filter 14 exceeds 80%, the function as a filter will not work.
- the filter 16 for filtration having the PET filter produced in this manner is welded to the inside surface of the container 20 made of a high-density polyethylene sheet by three sides each, so that the PET filter is produced.
- a filter 16 for filtration having 14 was fixed between two high density polyethylene sheets.
- the polyethylene injection port 30 and the injection port 32 were attached to the high-density polyethylene sheet by welding, and a polyvinyl chloride tube 36 was attached to each port.
- the edges of the high-density polyethylene sheet other than the portions sandwiching the filter 16 for filtering by the PET are welded together, so that the filter 16 for filtering by the PET filter 14 is inside the container 20.
- a container 22 with a filter welded to was prepared.
- Compressed air of 0.01 MPa was introduced from the polyvinyl chloride tube 36 of the prepared filter-equipped container 22 through the injection port 30. At this time, the pouring port 32 on the pouring side was opened, and the bag internal pressure was adjusted to 0.01 MPa. Although the air leakage from the welded part was confirmed by putting the swollen filter-equipped container 22 in water, the air leak from the filter-equipped container 22 was not observed.
- the filtration filter 16 includes the first welding frame 10, the second welding frame 12 arranged to face the first welding frame 10 in the thickness direction, and the outer peripheral portion of the first welding frame 10. And a filter 14 welded in a state of being sandwiched between the entire periphery of the second welding frame 12 and the entire periphery of the second welding frame 12. Then, the first welding frame 10 is formed of a flexible film made of a polymer and having a thickness of 120 ⁇ m or more into a frame shape having a first passage hole 10A penetrating in the thickness direction inside, and at the same time, the second welding frame 12 is formed.
- the filter 14 is made of a material having a melting point higher than that of the first welding frame 10 and the second welding frame 12 and having a hole portion, and the opening ratio of the hole portion is 10% or more and 80% or less.
- the melted first welding frame 10 and the second welding frame 12 from both sides of the filter 14 penetrate through the holes 14A of the unmelted filter 14 to be connected. Therefore, the first welding frame 10 and the second welding frame 12 facing each other can be coupled to each other to firmly fix the filter 14.
- the filter 14 and the first welding frame 10 and the second welding frame 12 are welded with high welding strength, so that the welding portion 34 is less likely to peel off, and the risk of fluid leakage from the welding portion 34 is reduced.
- first welding frame 10 and the second welding frame 12 are made of high density polyethylene having a melting point of 120 ° C. to 140 ° C., linear low density polyethylene having a melting point of 105 ° C. to 125 ° C., or a mixture thereof.
- the welded portion 34 is more unlikely to be peeled off because the welded portion 34 is made of the polymer containing the welded portion 34.
- first welding frame 10 and the second welding frame 12 are made of high density polyethylene having a melting point of 120 ° C. to 140 ° C., linear low density polyethylene having a melting point of 105 ° C. to 125 ° C., or a mixture thereof.
- the welded portion 34 is more unlikely to come off by being made of such a polymer.
- the fluid in the filtration target is movable in the order of the first passage hole 10A, the portion of the filter 14 that is not in contact with any of the first welding frame 10 and the second welding frame 12, and the second passage hole 12A. Since the first welding frame 10, the filter 14, and the second welding frame 12 are welded in the form, the fluid can be reliably flowed to the filter 14.
- the strength of the filter 14 itself can be secured by configuring the filter 14 to include at least one type of polyester, polyamide, polyolefin, polyether ether ketone, polyether sulfone, carbon fiber, and metal.
- the filter 14 is woven or knitted, so that the filter 14 has flexibility.
- the filter-equipped container 22 is welded so as to partition the inside of the polymer-made container 20 so that the filter-equipped container 22 can be easily shaken or rubbed, and the cell suspension. It becomes easy to remove the foreign matter contained in the suspension.
- the pore diameter of the pores 14A in the filter 14 is 10 to 200 ⁇ m, it is possible to capture impurities and target cells while preventing clogging of the filter 14.
- the filter 14 is formed in a rectangular sheet shape, processing when manufacturing the filter 14 becomes easy.
- the shapes of the first welding frame 10 and the second welding frame 12 are substantially the same, the work efficiency at the time of producing the filter 16 for filtration can be improved.
- the container 20 is configured by facing two rectangular polymer sheets of substantially the same shape and welding the peripheral portions of the polymer sheets together, and in the filter 16 for filtration provided inside the container 20.
- the first welding frame 10 is welded to one polymer sheet of the container 20 by a substantially U-shaped first welding portion 24, one of which is open when viewed in the thickness direction, and the second welding of the filtration filter 16 is performed.
- the frame 12 is welded to the other polymer sheet of the container 20 by a substantially U-shaped second welded portion 26, the other of which is open when viewed in the thickness direction, and the opening of the first welded portion 24 and the second welded portion.
- the opening of the portion 26 is oriented in the opposite direction. Therefore, the fluid flowing from the opening of the first welding portion 24 to the filter 14 flows to the outside from the opening of the second welding portion 26 without largely changing the flow direction. That is, since the fluid flows easily, it is possible to efficiently capture the contaminants and the target cells.
- the cell suspension is injected into one compartment S1 inside the container 20 and the cell suspension in which foreign matter is removed from the other compartment S2. It becomes easy to collect the suspension.
- the cell suspension is injected into one of the compartments S1 inside the container 20 in the filter-equipped container 22, the cells are filtered by the filtration filter 16, and the liquid for resuspending the cells is injected into the one compartment S1. Then, the cells can be resuspended, and the cell suspension can be easily collected from the one compartment S1.
- the filter 14 is sandwiched between the entire circumference of the first welding frame 10 and the entire circumference of the second welding frame 12 so that the first welding frame 10 and the second welding frame 12 respectively have the first welding frame. 10 or the pores of the filter 14 in which the molten polymer of the first welding frame 10 and the second welding frame 12 is not melted by being welded at a temperature higher than the melting point of the second welding frame 12 and lower than the melting point of the filter 14. 14A, and then the polymer can be solidified by, for example, heat dissipation.
- the polymer of the first welding frame 10 and the second welding frame 12 that has entered the hole 14A serves as an anchor, so to speak, so that the welding strength between the filter 14 and the first welding frame 10 and the second welding frame 12 is improved. be able to.
- the filter-equipped container 22 described above is configured such that the filter 14 is sandwiched between the first welding frame 10 and the second welding frame 12, the configuration is not limited to this, and the container 20 is not shown.
- the filter 14 is directly welded to the inside of the container 20 so as to divide the space, and the space formed by one surface of the filter 14 and the inside of the container 20 is constituted by the other surface of the filter 14 and the inside of the container 20.
- the inside of the container 20 may be partitioned by the filter 14 in such a manner that a flow path for moving the fluid to the space to be formed does not occur other than the filter 14. With such a configuration, it is possible to function as a filter while reducing the number of components.
- the filter-equipped container 22 in which the filtration filter 16 of the technique of the present disclosure is incorporated in the container 20 is used, for example, to remove foreign substances in cell suspension or to wash cells. Therefore, the filter 16 for filtration of the technique of this indication and the container 22 with a filter using the same can be utilized in, for example, the manufacturing industry of filtration equipment.
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Abstract
Description
i)第1溶着枠10の枠内の第1通過孔10A
ii)フィルター14のうち、第1溶着枠10及び第2溶着枠12のそれぞれの枠部10B、12Bに接していない部分
iii)第2溶着枠12の枠内の第2通過孔12A
の順、またはその逆の順にろ過対象物中の流体が移動可能な形態で、第1溶着枠10、フィルター14、及び第2溶着枠12が溶着されていることが好ましい。
ポリマーフィルムの融点測定は、DSC(TA Instruments製、Q20)にて行った。DSC測定条件は、窒素雰囲気下で(50ml/min)、測定温度範囲は30℃~200℃、昇温速度は10℃/minであった。ポリマーフィルムの融点としては、DSCでの融解でみられる吸熱ピークのピーク位置の温度を用いた。
ポリマーフィルムの密度測定は、液中秤量法にて行った。液中秤量法は、天秤(Mettler Toledo製、Blance XS105)に、比重測定治具(Mettler Toledo製)を取り付け、ポリマーフィルムを空気中で秤量した後、エタノール中で秤量した。液温を測定し、文献(Dweight E.Gray,American Institute of Physics Handbook,McGraw-Hill Book Company Inc.,1957)に記載された方法により、エタノールの密度を求め、次式によりポリマーフィルムの密度を計算した。
ρ={A/(A-B)}×(ρ0-d)+d
ポリマーフィルムの膜厚は、フィルムメーカーが提示したカタログ値を用いた。
フィルターの開孔率は、フィルターメーカーが提示したカタログ値を用いた。
フィルターの孔部の孔径は、フィルターメーカーが提示したカタログ値を用いた。
フィルターの繊維径は、フィルターメーカーが提示したカタログ値を用いた。
第1溶着枠10を構成する第1のポリマーフィルムと第2溶着枠12を構成する第2のポリマーフィルムとの間に、フィルター14の外周が全周にわたって挟み込まれた状態で、ポリエチレンフィルムの融点以上であってPET製のフィルター14の融点以下である230℃に設定したインパルスシーラー(石崎電機製 SURE NL-102JW)を用いて加熱することにより、第1溶着枠10、フィルター14及び第2溶着枠12とを、溶着部34を介して溶着させた。溶着部34は、フィルター14の第1溶着枠10及び第2溶着枠12に挟み込まれた部分の全周にわたって形成させた。こうして作製したろ過用フィルター16は、フィルターにおける第1溶着枠10及び第2溶着枠12に挟まれていない部分、すなわち第1通過孔10Aと第2通過孔12Aとに対応した部分がフィルターとしての機能を発揮する。ここで、ろ過用フィルター16は、第1溶着枠10を構成する第1のポリマーフィルム、第2溶着枠12を構成する第2のポリマーフィルム及びフィルター14の材質を種々変えて、下記実施例1~18、比較例1~18に記載の材質のものを作製した。なお、比較例19~20は、第1のポリマーフィルムと第2溶着枠12を構成する第2のポリマーフィルムとを溶着させたものであり、ろ過用フィルター16には相当しないが、同様にして溶着強度を評価した。
ろ過用フィルター16における溶着部34の溶着強度を以下に詳述する評価モデルを用いて測定した。
溶着強度の測定はJIS Z0238を参考にした剥離試験によって実施した。インパルスシーラーで溶着させたろ過用フィルター16の溶着部34を、長さ25mm、幅15mmの大きさで3個切出した。これを引張試験機(Ez-Test-Ez-SX、島津製作所製)による180°剥離試験法に供した。ここで、引張試験機は引張速度を10mm/sec、チャック間距離を20mm/secに設定した。溶着強度は、溶着部34の剥離もしくは破断が起こるまでの最大の荷重として求め(N/15mm)、N=3の平均値で評価した。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET(ポリエチレンテレフタレート)製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は32.6N/15mmであった。
高密度ポリエチレンフィルム(HDPE、細川洋行製 POLYELITE EH、Tm=126℃、密度0.947g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は33.3N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は30.5N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、東洋紡製 L4102、Tm=123℃、密度0.907g/cm3、膜厚100μm、L4102、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は33.7N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、タマポリ製 SE620L、Tm=113℃、密度0.921g/cm3、膜厚140μm、SE620L、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が280μmのフィルムを調製した。膜厚を280μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は26.7N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、タマポリ製 SE620L、Tm=113℃、密度0.921g/cm3、膜厚140μm、SE620L、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は20.2N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は13.9N/15mmであった。
低密度ポリエチレンフィルム(LDPE、細川洋行製 POLYELITE EL、Tm=115℃、密度0.907g/cm3、膜厚250μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ28mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は13.9N/15mmであった。
低密度ポリエチレンフィルム(LDPE、サンプラテック製、Tm=110℃、密度0.915g/cm3、膜厚300μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は14.9N/15mmであった。
低密度ポリエチレンフィルム(LDPE、サンプラテック製、Tm=111℃、密度0.915g/cm3、膜厚500μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は14.4N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は12.3N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、東洋紡製 L4102、Tm=123℃、密度0.907g/cm3、膜厚100μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 No.T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は16.0N/15mmであった。
実施例1から6及び比較例1から6の結果を表1として整理する。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にナイロン製のフィルター14(Sefar製 03-30/18、孔径30μm、繊維径40μm、開孔率18%、Tm=252℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は29.0N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にナイロン製のフィルター14(Sefar製 03-30/18、孔径30μm、繊維径40μm、開孔率18%、Tm=252℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は23.4N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にナイロン製のフィルター14(Sefar製 03-30/18、孔径30μm、繊維径40μm、開孔率18%、Tm=252℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は12.0N/15mmであった。
実施例7、8及び比較例7の結果を表2として整理する。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 PET24、孔径21μm、繊維径41μm、開孔率12%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は24.2N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(Sefar製 PET24、孔径21μm、繊維径41μm、開孔率12%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は24.2N/15mmであった。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 07-27/19、孔径27μm、繊維径35μm、開孔率19%、Tm=256℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は32.7N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(Sefar製 07-27/19、孔径27μm、繊維径35μm、開孔率19%、Tm=256℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は29.4N/15mmであった。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は32.6N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(NBCメッシュテック製 T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は30.5N/15mmであった。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 T-180T、孔径86μm、繊維径55μm、開孔率37%、Tm=255℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は52.2N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(NBCメッシュテック製 T-180T、孔径86μm、繊維径55μm、開孔率37%、Tm=255℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は35.8N/15mmであった。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 T-100T、孔径183μm、繊維径71μm、開孔率52%、Tm=255℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は53.6N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(NBCメッシュテック製 T-100T、孔径183μm、繊維径71μm、開孔率52%、Tm=255℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は35.6N/15mmであった。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 PET6-HD、孔径6μm、繊維径34μm、開孔率5%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は13.1N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(Sefar製 PET6-HD、孔径6μm、繊維径34μm、開孔率5%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は12.3N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 PET6-HD、孔径6μm、繊維径34μm、開孔率5%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は6.0N/15mmであった。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 PET15、孔径15μm、繊維径37μm、開孔率9%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は18.5N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした2枚の線状低密度ポリエチレンフィルムの間にPET製のフィルター14(Sefar製 PET15、孔径15μm、繊維径37μm、開孔率9%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は17.1N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 PET15、孔径15μm、繊維径37μm、開孔率9%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は8.1N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 PET24、孔径21μm、繊維径41μm、開孔率12%、Tm=257℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は11.1N/15mmであった。
<比較例15>
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(Sefar製 07-27/19、孔径27μm、繊維径35μm、開孔率19%、Tm=256℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は13.8N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 T-380T、孔径28μm、繊維径35μm、開孔率23%、Tm=254℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は13.9N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 T-180T、孔径86μm、繊維径55μm、開孔率37%、Tm=255℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は21.6N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)2枚の間にPET製のフィルター14(NBCメッシュテック製 T-100T、孔径183μm、繊維径71μm、開孔率52%、Tm=255℃、長さ80mm、幅15mm)を挟み込み、この状態で溶着させてろ過用フィルター16を得た。その溶着部34の溶着強度は32.1N/15mmであった。
実施例9から18及び比較例8から18の結果を表3として整理する。
高密度ポリエチレンフィルム(HDPE、タマポリ製 HD、Tm=131℃、密度0.912g/cm3、膜厚200μm、長さ80mm、幅15mm)を2枚重ね、両面溶着をした後、その溶着強度を測定した。溶着強度は51.8N/15mmであった。
線状低密度ポリエチレンフィルム(LLDPE、三井化学東セロ製 HC#100、Tm=124℃、密度0.922g/cm3、膜厚100μm、長さ80mm、幅15mm)を2枚重ねて溶着し、膜厚が200μmのフィルムを調製した。こうして膜厚を200μmとした線状低密度ポリエチレンフィルムを2枚重ね、両面溶着をした後、その溶着強度を測定した。溶着強度は33.5N/15mmであった。
低密度ポリエチレンフィルム(LDPE、タマポリ製 V-2、Tm=112℃、密度0.923g/cm3、膜厚200μm、長さ80mm、幅15mm)を2枚重ね、両面溶着をした後、その溶着強度を測定した。溶着強度は27.9N/15mmであった。
比較例19から21の結果を表4として整理する。
フィルムの材料とPET製のフィルター14との溶着強度の関係については、表1からわかるように、フィルムの材料としてHDPEを用いたものが最も溶着強度が高く、LDPEを用いたものが最も低く、LLDPEを用いたものはその間の溶着強度であることがわかる。同様なことは、フィルター14の材料としてナイロンを用いた表2の結果からもわかる。
D.フィルター付容器の作製
高密度ポリエチレンフィルム(HDPE、細川洋行製、POLYELITE EH、Tm=126℃、密度0.947g/cm3、膜厚200μm)からなる枠(サイズ:外枠300mm×200mm、内枠270mm×170mm、枠幅は両端15mmずつ)の間に、PET製のフィルター14(Sefar製 07-27/19、孔径27μm、開孔率19%、Tm=256℃)を挟み、周辺部をインパルスシーラーを用い、230℃で溶着を行った。こうして作製したPET製のフィルターを有するろ過用フィルター16を図4に示すように、高密度ポリエチレンシート製の容器20の内側の面に対して、それぞれ3辺ずつ溶着することで、PET製のフィルター14を有するろ過用フィルター16を2枚の高密度ポリエチレンシート間に固定した。また、ポリエチレン製の注入用ポート30及び注出用ポート32を高密度ポリエチレンシートに溶着で取り付けたうえ、各ポートにはポリ塩化ビニル製のチューブ36を取り付けた。最後に、高密度ポリエチレンシートのうち、PET製のフィルター14によるろ過用フィルター16を挟む部分以外の辺縁部同士を溶着することで、PET製のフィルター14によるろ過用フィルター16が容器20の内部に溶着されたフィルター付容器22を作製した。
次に、本実施形態の作用並びに効果を説明する。
Claims (18)
- ポリマーを含む膜厚120μm以上の可撓性フィルムにて構成され、厚さ方向に貫通された第1通過孔を内側に有する枠状に形成された第1溶着枠と、
ポリマーを含む膜厚120μm以上の可撓性フィルムにて構成され、前記第1溶着枠と厚さ方向にて対向して配置され、前記第1通過孔に対応した位置に厚さ方向に貫通された第2通過孔を内側に有する枠状に形成された第2溶着枠と、
前記第1溶着枠及び前記第2溶着枠より高い融点をもちかつ孔部を備え当該孔部による開孔率が10%以上80%以下とされた材質にて構成されると共に、外周部が前記第1溶着枠の全周と前記第2溶着枠の全周との間に挟み込まれた状態で前記第1溶着枠と前記第2溶着枠とにそれぞれ溶着されたフィルターと、を有し、
前記第1溶着枠が、融点が120℃~140℃である高密度ポリエチレン、融点が105℃~125℃である線状低密度ポリエチレン、又は前記高密度ポリエチレン及び前記線状低密度ポリエチレンの少なくとも一方を混合した混合物を含むポリマーにより構成されている、
ろ過用フィルター。 - 前記第1溶着枠が、融点が120℃~140℃である高密度ポリエチレン、融点が105℃~125℃である線状低密度ポリエチレン、又は前記高密度ポリエチレン及び前記線状低密度ポリエチレンの少なくとも一方をそれぞれを混合した混合物からなるポリマーにより構成されている、
請求項1に記載のろ過用フィルター。 - 前記第1通過孔、前記フィルターにおける前記第1溶着枠及び前記第2溶着枠のいずれにも接していない部分、前記第2通過孔、の順にろ過対象物中の流体が移動可能な形態で前記第1溶着枠、前記フィルター、及び前記第2溶着枠が溶着されている、
請求項1又は請求項2に記載のろ過用フィルター。 - 前記第2溶着枠が、融点が120℃~140℃である高密度ポリエチレン、融点が105℃~125℃である線状低密度ポリエチレン、又は前記高密度ポリエチレン及び前記線状低密度ポリエチレンの少なくとも一方を混合した混合物を含むポリマーにより構成されている、
請求項1~請求項3のいずれか一項に記載のろ過用フィルター。 - 前記第2溶着枠が、融点が120℃~140℃である高密度ポリエチレン、融点が105℃~125℃である線状低密度ポリエチレン、又は前記高密度ポリエチレン及び前記線状低密度ポリエチレンの少なくとも一方を混合した混合物からなるポリマーにより構成されている、
請求項1~請求項3のいずれか一項に記載のろ過用フィルター。 - 前記フィルターが、ポリエステル、ポリアミド、ポリオレフィン、ポリエーテルエーテルケトン、ポリエーテルスルフォン、炭素繊維、及び金属の少なくとも一種類を含んでいる、
請求項1~請求項5のいずれか一項に記載のろ過用フィルター。 - 前記フィルターが織物または編物である、
請求項1~請求項6のいずれか一項に記載のろ過用フィルター。 - 前記フィルターにおける前記孔部の孔径が5~200μmである、
請求項1~請求項7のいずれか一項に記載のろ過用フィルター。 - 前記フィルターが矩形シート状である、
請求項1~請求項8のいずれか一項に記載のろ過用フィルター。 - 前記第1溶着枠及び前記第2溶着枠の形状が略同一である、
請求項1~請求項9のいずれか一項に記載のろ過用フィルター。 - 前記フィルターは、前記第1溶着枠の全周と前記第2溶着枠の全周との間に挟み込まれた状態で、前記第1溶着枠及び前記第2溶着枠に、それぞれ前記第1溶着枠又は前記第2溶着枠の融点以上前記フィルターの融点以下の温度で加熱することにより溶着されている、
請求項1~請求項10のいずれか一項に記載のろ過用フィルター。 - 請求項1から請求項11のいずれか一項に記載のろ過用フィルターが、ポリマーからなる容器の内部を区画するように溶着されている、
フィルター付容器。 - 前記容器が略同一形状の矩形状の可撓性ポリマーシートを2枚対向しかつ当該ポリマーシートの周縁部同士を溶着して構成されており、
前記容器の内部に設けられた前記ろ過用フィルターにおける前記第1溶着枠が、前記容器の一方の前記可撓性ポリマーシートに厚さ方向視にて一方が開放される略U字状の第1溶着部により溶着されており、
前記ろ過用フィルターにおける前記第2溶着枠が、前記容器の他方の前記可撓性ポリマーシートに厚さ方向視にて他方が開放される略U字状の第2溶着部により溶着されており、
前記第1溶着部の開口と前記第2溶着部の開口とが、反対側の向きとされている、
請求項12に記載のフィルター付容器。 - 前記容器に注入用ポート及び注出用ポートの少なくとも一方を備えた、
請求項12又は請求項13に記載のフィルター付容器。 - 前記ろ過用フィルターにより区画された前記容器の内部の一方に注入用ポートを、他方に注出用ポートを備えた、
請求項14に記載のフィルター付容器。 - ポリマーシートを2枚対向しかつ当該ポリマーシートの周縁部同士を溶着して構成された容器と、
前記容器の内部を区画するように前記容器の内部に溶着されていると共に、孔部を備え当該孔部による開孔率が10%以上80%以下とされた材質にて構成されたフィルターと、
を有するフィルター付容器。 - 請求項12から請求項16のいずれか一項に記載の前記フィルター付容器における容器内部の一方の区画に細胞懸濁液を注入し、この細胞懸濁液をろ過用フィルターに通過させ、他方の区画から細胞を含むろ液を回収する工程を含む、
細胞懸濁液に含まれる異物を除く方法。 - 請求項12から請求項16のいずれか一項に記載の前記フィルター付容器における容器内部の一方の区画に細胞懸濁液を注入し、この細胞懸濁液をろ過用フィルターに通過させ、他方の区画から細胞を含むろ液を回収し、更に、当該一方の区画に細胞懸濁用の溶液を注入し、同区画に残存する細胞を再懸濁させ、この再懸濁させた細胞懸濁液をろ過用フィルターに通過させて他方の区画から細胞を含むろ液を回収する工程を含む、
細胞懸濁液に含まれる異物を除く方法。
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