WO2020047904A1 - 生物样本处理装置 - Google Patents

生物样本处理装置 Download PDF

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Publication number
WO2020047904A1
WO2020047904A1 PCT/CN2018/106460 CN2018106460W WO2020047904A1 WO 2020047904 A1 WO2020047904 A1 WO 2020047904A1 CN 2018106460 W CN2018106460 W CN 2018106460W WO 2020047904 A1 WO2020047904 A1 WO 2020047904A1
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WO
WIPO (PCT)
Prior art keywords
biological sample
upper cover
processing device
sample processing
bottle
Prior art date
Application number
PCT/CN2018/106460
Other languages
English (en)
French (fr)
Inventor
尤其敏
王震
周艳琼
杨帆
赵芯
孙刚
杨翔宇
赵雁林
赵冰
欧喜超
平国华
吴蓓蓓
Original Assignee
杭州优思达生物技术有限公司
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Publication date
Application filed by 杭州优思达生物技术有限公司 filed Critical 杭州优思达生物技术有限公司
Priority to GB2103090.3A priority Critical patent/GB2590574B/en
Priority to JP2021512783A priority patent/JP7188823B2/ja
Publication of WO2020047904A1 publication Critical patent/WO2020047904A1/zh
Priority to US17/249,587 priority patent/US20210190643A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/18—Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/047—Additional chamber, reservoir
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1418—Depression, aspiration
    • G01N2001/1427—Positive displacement, piston, peristaltic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Definitions

  • the invention relates to a biological sample processing device.
  • the collection and pretreatment of biological samples is an important part of clinical testing, which directly affects the accuracy of subsequent test results.
  • the current collection method of biological samples is to collect the samples (blood, urine, sputum, feces, etc.) into corresponding containers.
  • the inspector opens the container and takes a sample from it, or adds a processing liquid to the container to complete the pretreatment process of the sample.
  • a major drawback of this process is that inspectors are directly exposed to aerosols at risk of infection when opening containers, increasing the risk of nosocomial infections. Therefore, there is a need for a safer sampling container that can protect inspectors from aerosol exposure.
  • the present invention provides a biological sample processing device that solves the above problems.
  • the present invention adopts the following technical solutions:
  • a biological sample processing device includes a containing bottle for holding a biological sample to be processed, an upper end of the containing bottle is formed with a receiving opening and a lower end is formed with a liquid outlet, and the biological sample processing device further includes an upper cover component and a lower cover;
  • the upper cap assembly is connected to the upper end of the receiving bottle which is formed with the receiving port;
  • the lower cap is connected to the lower end of the receiving bottle which is formed with the liquid outlet;
  • the upper cap assembly includes an upper cap, a lower seal, a piston and a piercing member;
  • the upper cap is formed with A through hole, a lower seal is provided at the lower end of the through hole formed by the upper cover so that the upper cover forms a cavity for receiving a solution that reacts with a biological sample;
  • the puncturing member is connected to the piston;
  • the inner wall of the through hole formed by the piston and the upper cover Closely fit and slide along the inner wall to drive the piercing member to pierce the lower seal;
  • the biological sample processing device further includes a filter sheet; the filter sheet is placed in a cavity formed by the containing bottle to divide the cavity formed by the containing bottle into a first cavity and a second cavity.
  • the accommodating bottle includes an upper connection portion, an elastically deformable bottle body, and a lower connection portion; the bottle body connects the upper connection portion and the lower connection portion; the upper connection portion is formed with a receiving port; and the lower connection portion is formed with a liquid outlet.
  • an external thread is formed on the outer side of the upper connection portion; a sleeve portion with an internal thread on the inner wall is integrally formed on the upper end of the upper cover; and an internal thread is provided on the inner wall of the sleeve portion to cooperate with the external thread formed on the outside of the upper connection portion.
  • a gap is formed between the upper cover and the bottle body.
  • the lower connecting part is funnel-shaped; the liquid outlet is located at the tip of the lower connecting part; the lower cover is provided with a funnel-shaped inner cavity which is matched with the lower connecting part.
  • the lower connection portion includes a first funnel portion, a cylindrical screw connection portion formed with external threads on the outside, and a second funnel portion; the first funnel portion is connected to the lower end of the bottle body; and one end of the screw connection portion is connected to the first funnel. And the other end is connected to the second funnel part; the liquid outlet is located at the tip of the second funnel part; Threaded internal threads.
  • one end of the threaded connection portion near the first funnel portion extends in a radial direction to form a support portion; the biological sample processing device further includes a filter sheet; the filter sheet is located in the lower connection portion and abuts against the support portion.
  • the lower ends of the lower caps are located in the same plane, and the plane is perpendicular to the central axis of the receiving bottle.
  • the upper cover assembly further includes an upper seal; the upper seal is disposed at the upper end of the through hole formed by the upper cover and cooperates with the lower seal to close the cavity surrounded by the upper cover and used for containing the solution reacting with the biological sample.
  • the invention is beneficial in that the provided biological sample processing device can realize the processing of biological samples without opening the upper cover.
  • the advantage of the invention is that the biological sample processing device provided can be used for centrifugally enriching the biological sample sediment and used for observation, cultivation or detection of pathogenic microorganisms.
  • FIG. 1 is an exploded view of a biological sample processing device according to the present invention
  • FIG. 2 is a cross-sectional view of the biological sample processing apparatus in FIG. 1;
  • FIG. 3 is a sectional view of another working state of the biological sample processing apparatus in FIG. 1;
  • Biological sample processing device 100 containing bottle 10, receiving port 11, liquid outlet 12, upper connection portion 13, bottle body 14, lower connection portion 15, first funnel portion 151, threaded connection portion 152, second funnel portion 153, The support portion 154, the upper cover assembly 20, the upper cover 21, the socket portion 211, the lower seal 22, the piston 23, the piercing member 24, the upper seal 25, the lower cover 30, and the filter sheet 40.
  • a biological sample processing apparatus 100 for processing a biological sample, wherein the biological sample may be blood, urine, sputum, feces, or the like.
  • the biological sample processing apparatus 100 includes a receiving bottle 10, an upper cap assembly 20 and a lower cap 30.
  • the receiving bottle 10 is used for containing the biological sample to be processed.
  • the receiving bottle 10 is formed with a receiving port 11 at the upper end and a liquid outlet 12 formed at the lower end.
  • the liquid outlet 12 is preferably a circular hole with an aperture ranging from 1-2 mm. , Preferably 1.5 mm.
  • the upper cap assembly 20 is connected to the upper end of the receiving bottle 10 formed with the receiving port 11 to close the receiving port 11, and the lower cap 30 is connected to the lower end of the receiving bottle 10 formed with the liquid discharging port 12 to close the liquid discharging port 12.
  • the upper cover assembly 20 includes an upper cover 21, a lower seal 22, a piston 23, and a puncture member 24.
  • the upper cover 21 is formed with a through hole
  • the lower seal 22 is disposed at the lower end of the through hole formed by the upper cover 21 so that the upper cover 21 forms a cavity for receiving a solution that reacts with a biological sample.
  • the lower seal 22 is a film or other
  • polypropylene aluminum foil composite film is preferred, with a diameter ranging from 25-30mm, preferably 28mm, and a thickness ranging from 0.04-0.06mm, preferably 0.05mm.
  • the piercing member 24 is connected to the piston 23, and the piston 23 closely cooperates with the inner wall of the through hole formed by the upper cover 21 to close the through hole, and the piston 23 can slide along the inner wall to drive the piercing member 24, wherein the piston 23 can be made of rubber
  • the surface of the piston 23 in contact with the inner wall of the through hole formed by the upper cover 21 is formed with a screw structure.
  • the piercing member 24 is preferably a cross-shaped piercing structure, and the material is preferably a polyvinyl chloride plastic, and the piercing angle ranges from 30 to 60 ° C, preferably 45 ° C.
  • the biological sample processing apparatus 100 further includes a filter 40.
  • the filter sheet 40 is placed in the cavity formed by the receiving bottle 10 to divide the cavity formed by the receiving bottle 10 into a first cavity and a second cavity.
  • the filter sheet 40 can be used to separate large particles of sediment.
  • the filter sheet 40 is preferably circular, and the pore diameter range is 2-5 mm, preferably 3 mm.
  • the receiving bottle 10 includes an upper connecting portion 13, a bottle body 14, and a lower connecting portion 15.
  • the bottle body 14 connects the upper connecting portion 13 and the lower connecting portion 15.
  • the upper connecting portion 13 is formed with a receiving port 11.
  • the lower connection portion 15 is formed with a liquid outlet 12.
  • the material of the containing bottle 10 is preferably a polypropylene resin, wherein the bottle body 14 can be elastically deformed, and the liquid in the containing bottle 10 can be squeezed out of the liquid outlet 12 by squeezing the bottle body 14 to ensure the bottle body 14 With a suitable elastic force, the thickness of the bottle body 14 ranges from 0.05 to 0.10 mm, preferably 0.07 mm.
  • an external thread is formed on the outer side of the upper connection portion 13.
  • the upper end of the upper cover 21 is integrally formed with a socket portion 211 with an internal thread on the inner wall.
  • the inner wall of the socket part 211 is provided with an internal screw thread that cooperates with an external screw thread formed outside the upper connection part 13.
  • a gap is formed between the upper cover 21 and the bottle body 14.
  • a ratio of a gap formed between the upper cover 21 and the bottle body 14 and an inner diameter of the bottle body 14 ranges from 0.05 to 0.2, and preferably 0.07.
  • the bottle body 14 when the bottle body 14 is squeezed to release the liquid contained in the bottle 10, the bottle body 14 is recessed inward.
  • the upper cover 21 and the bottle body 14 are required. A sufficiently large gap is formed.
  • the gap between the upper cover 21 and the bottle body 14 affects the volume of the cavity in the upper cover 21 for containing the solution reacting with the biological sample, and is not easy to be too small.
  • the ratio of the gap formed between the upper lid 21 and the bottle body 14 and the inner diameter of the bottle body 14 is greater than or equal to 0.05 and less than or equal to 0.2.
  • the volume of the inner cavity of the upper lid 21 is sufficiently large while ensuring that the bottle body 14 is sufficiently inward. Depressed space.
  • the lower connecting portion 15 is funnel-shaped.
  • the liquid outlet 12 is located at the tip of the lower connection portion 15.
  • the lower cover 30 is provided with a funnel-shaped inner cavity which cooperates with the lower connecting portion 15.
  • the lower connection portion 15 includes a first funnel portion 151, a cylindrical screw connection portion 152 formed with an external thread on the outside, and a second funnel portion 153.
  • the first funnel portion 151 is connected to the lower end of the bottle body 14.
  • One end of the threaded connection portion 152 is connected to the first funnel portion 151 and the other end is connected to the second funnel portion 153.
  • the liquid outlet 12 is located at the tip of the second funnel portion 153.
  • the inner wall of the funnel-shaped inner cavity of the lower cover 30 that cooperates with the lower connection portion 15 is provided with an internal thread that cooperates with an external thread formed on the outside of the screw connection portion 152.
  • the slope range of the first funnel portion 151 is 30-60 ° C, preferably 45 ° C
  • the slope range of the second funnel portion 153 is 30-60 ° C, preferably 45 ° C, so that the sample can be completely collected at the bottom of the containing bottle 10 .
  • the biological sample processing apparatus 100 further includes a filter sheet 40.
  • the filter sheet 40 is located in the lower connection portion 15 and abuts against the support portion 154.
  • the lower ends of the lower caps 30 are located in the same plane, and the plane is perpendicular to the central axis of the receiving bottle 10.
  • the lower end of the lower cover 30 is located in the same plane, and the plane is perpendicular to the central axis of the accommodating bottle 10, so that the biological sample processing device 100 can stably stand on the water platform surface through the lower cover 30.
  • the upper cover assembly 20 further includes an upper seal 25.
  • the upper seal 25 is disposed at the upper end of the through hole formed by the upper cover 21 and cooperates with the lower seal 22 to close the cavity surrounded by the upper cover 21 for containing a solution reacting with the biological sample.
  • the upper seal 25 and the lower seal 22 completely seal the cavity surrounded by the upper cover 21 for containing the solution reacting with the biological sample, so that the solution reacting with the biological sample is completely isolated from the outside.
  • the upper seal 25 can also prevent the piston 23 from being pressed by mistake.
  • the use steps of the biological sample processing device 100 are as follows: the user unscrews the upper cover 21, collects the biological sample in the containing bottle 10, and screwes the upper cover 21 tightly. Tear the upper seal 25, push the piston 23, so that the cross-shaped puncture member 24 at the lower part of the piston 23 pierces the lower seal 22, and the solution reacting with the biological sample enters the containing bottle 10 and is mixed with the biological sample in it, and is left at room temperature for 20-30 minutes .
  • the biological sample processing device 100 was placed in a centrifuge, and centrifuged at 5000 g for 15 minutes at room temperature. Unscrew the lower cover 30, squeeze the elastic bottle body 14, the precipitate is filtered by the filter 40, and the supernatant flows out from the liquid outlet 12.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hydrology & Water Resources (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

一种生物样本处理装置（100），包括容纳瓶（10），上盖组件（20）和下盖（30）；容纳瓶（10）的上端形成有接纳口（11）且下端形成有出液口（12）；上盖组件（20）连接至容纳瓶（10）的形成有接纳口（11）的上端；下盖（30）连接至容纳瓶（10）的形成有出液口（12）的下端；上盖组件（20）包括上盖（21）、下密封件（22）、活塞（23）和穿刺件（24）；上盖（21）形成有通孔，下密封件（22）设置于上盖（21）形成的通孔的下端以使上盖（21）形成一用于容纳与生物样本反应的溶液的腔；活塞（23）与上盖（21）形成的通孔的内壁紧密配合且可沿该内壁滑动以带动穿刺件（24）刺破下密封件（22）。

Description

生物样本处理装置 技术领域
本发明涉及一种生物样本处理装置。
背景技术
生物样本的采集和预处理是临床检验的重要环节，直接影响后续检测结果的准确性。目前生物样本的采集方式是将样本(血液、尿液、痰液、粪便等)收集到相应的容器中。检验人员打开容器，从中取出样本，或向容器中添加处理液，完成样本的预处理过程。该流程存在的一个主要缺陷是，检验人员在打开容器时直接暴露在有传染风险的气溶胶中，增加了院内感染的风险。因此，临床需要一种更加安全的，能保护检验人员避免气溶胶暴露的采样容器。
发明内容
为解决现有技术的不足，本发明提供了一种解决上述问题的生物样本处理装置。
为了实现上述目标，本发明采用如下的技术方案：
一种生物样本处理装置，包括容纳瓶，用于盛放待处理的生物样本，容纳瓶的上端形成有接纳口且下端形成有出液口，生物样本处理装置还包括上盖组件和下盖；上盖组件连接至容纳瓶的形成有接纳口的上端；下盖连接至容纳瓶的形成有出液口的下端；上盖组件包括上盖、下密封件、活塞和穿刺件；上盖形成有通孔，下密封件设置于上盖形成的通孔的下端以使上盖形成一用于容纳与生物样本反应的溶液的腔；穿刺件连接至活塞；活塞与上盖形成的通孔的内壁紧密配合且可沿该内壁滑动以带动穿刺件刺破下密封件；上盖至少部分位于容纳瓶所形成的腔内。
进一步地，生物样本处理装置还包括过滤片；过滤片置于容纳瓶所形成的腔内以将容纳瓶所形成的腔分割成第一腔和第二腔。
进一步地，容纳瓶包括上连接部、可弹性形变的瓶体和下连接部；瓶体连接上连接部和下连接部；上连接部形成有接纳口；下连接部形成有出液口。
进一步地，上连接部外侧形成有外螺纹；上盖的上端一体形成有内壁设有内螺纹的套接部；套接部内壁设有与形成于上连接部外侧的外螺纹配合的内螺纹。
进一步地，上盖与瓶体之间形成有间隙。
进一步地，下连接部为漏斗形；出液口位于下连接部的尖端；下盖设有与下连接部相配合的漏斗形内腔。
进一步地，下连接部包括第一漏斗部、外侧形成有外螺纹的圆筒形的螺纹连接部和第二 漏斗部；第一漏斗部连接至瓶体下端；螺纹连接部一端连接至第一漏斗部且另一端连接至第二漏斗部；出液口位于第二漏斗部的尖端；下盖的与下连接部相配合的漏斗形内腔的内壁上设有与形成于螺纹连接部外侧的外螺纹相配合的内螺纹。
进一步地，螺纹连接部靠近第一漏斗部的一端沿径向方向延伸形成支撑部；生物样本处理装置还包括过滤片；过滤片位于下连接部内并抵靠支撑部。
进一步地，下盖的下端位于同一平面内，且该平面与容纳瓶的中心轴线垂直。
进一步地，上盖组件还包括上密封件；上密封件设置于上盖形成的通孔的上端且与下密封件配合以封闭上盖围成的用于容纳与生物样本反应的溶液的腔。
本发明的有益之处在于提供的生物样本处理装置无需打开上盖即可实现对生物样本的处理。
发明的有益之处在于提供的生物样本处理装置可离心富集生物样本沉淀物，并用于病原微生物观察、培养或检测。
附图说明
图1是本发明的一种生物样本处理装置的***图；
图2是图1中的生物样本处理装置的剖视图；
图3是图1中的生物样本处理装置的另一工作状态的剖视图；
生物样本处理装置100，容纳瓶10，接纳口11，出液口12，上连接部13，瓶体14，下连接部15，第一漏斗部151，螺纹连接部152，第二漏斗部153，支撑部154，上盖组件20，上盖21，套接部211，下密封件22，活塞23，穿刺件24，上密封件25，下盖30，过滤片40。
具体实施方式
以下结合附图和具体实施例对本发明作具体的介绍。
如图1至图3所示，一种用于处理生物样本的生物样本处理装置100，其中，生物样本可为血液、尿液、痰液、粪便等。该生物样本处理装置100包括容纳瓶10、上盖组件20和下盖30。其中，容纳瓶10用于盛放待处理的生物样本，容纳瓶10的上端形成有接纳口11且下端形成有出液口12，该出液口12优选为圆形孔，孔径范围1-2mm，优选1.5mm。上盖组件20连接至容纳瓶10的形成有接纳口11的上端以封闭接纳口11，下盖30连接至容纳瓶10的形成有出液口12的下端以封闭出液口12。
具体而言，上盖组件20包括上盖21、下密封件22、活塞23和穿刺件24。上盖21形成有通孔，下密封件22设置于上盖21形成的通孔的下端以使上盖21形成一用于容纳与生物样本反应的溶液的腔，下密封件22为薄膜或其他易被戳破的材料制备，优选聚丙烯铝箔复合膜，直径范围25-30mm，优选28mm，厚度范围0.04-0.06mm，优选0.05mm。穿刺件24连接至 活塞23，活塞23与上盖21形成的通孔的内壁紧密配合以封闭通孔，且活塞23可沿该内壁滑动以带动穿刺件24运动，其中，活塞23可由橡胶制成，且活塞23与上盖21形成的通孔的内壁接触的表面形成有螺纹结构。当穿刺件24向上盖21下端运动时可刺破下密封件22，此时存储于上盖21中的与生物样本反应的溶液流入容纳瓶10内与位于该容纳瓶10内的生物样本反应，上盖21至少部分位于容纳瓶10所形成的腔内。其中，穿刺件24优选为十字形穿刺结构，材料优选聚氯乙烯塑料，穿刺角范围30-60℃，优选45℃。
作为一种优选的实施方式，生物样本处理装置100还包括过滤片40。过滤片40置于容纳瓶10所形成的腔内以将容纳瓶10所形成的腔分割成第一腔和第二腔。
具体而言，过滤片40可用于分隔大颗粒沉淀物，过滤片40优选圆形，孔径范围2-5mm，优选3mm。
作为一种优选的实施方式，容纳瓶10包括上连接部13、瓶体14和下连接部15，瓶体14连接上连接部13和下连接部15，上连接部13形成有接纳口11，下连接部15形成有出液口12。
具体而言，容纳瓶10材料优选聚丙烯树脂，其中，瓶体14可弹性变形，可通过挤压瓶体14将容纳瓶10中的液体从出液口12挤压出去，为保证瓶体14具有合适的弹性力，瓶体14厚度范围0.05-0.10mm，优选0.07mm。
作为一种优选的实施方式，上连接部13外侧形成有外螺纹。上盖21的上端一体形成有内壁设有内螺纹的套接部211。套接部211的内壁设有与形成于上连接部13外侧的外螺纹配合的内螺纹。安装上盖21时，将套接部211套在上连接部13上，再旋转上盖21将上盖21安装至容纳瓶10。
作为一种优选的实施方式，上盖21与瓶体14之间形成有间隙。上盖21与瓶体14之间形成的间隙和瓶体14的内径的比值范围为大于等于0.05小于等于0.2，优选为0.07。
具体而言，当挤压瓶体14释放容纳瓶10内的液体时，瓶体14向内凹陷，为了保证瓶体14有足够的向内凹陷的空间，上盖21与瓶体14之间需要形成有足够大的间隙，同时，上盖21与瓶体14之间的间隙影响上盖21内用于容纳与生物样本反应的溶液的腔的体积，不易过小。上盖21与瓶体14之间形成的间隙和瓶体14的内径的比值范围为大于等于0.05小于等于0.2可以实现上盖21内腔的体积足够大的同时保证瓶体14有足够的向内凹陷的空间。
作为一种优选的实施方式，下连接部15为漏斗形。出液口12位于下连接部15的尖端。下盖30设有与下连接部15相配合的漏斗形内腔。
作为一种优选的实施方式，下连接部15包括第一漏斗部151、外侧形成有外螺纹的圆筒形的螺纹连接部152和第二漏斗部153。第一漏斗部151连接至瓶体14下端。螺纹连接部152 一端连接至第一漏斗部151且另一端连接至第二漏斗部153。出液口12位于第二漏斗部153的尖端。下盖30的与下连接部15相配合的漏斗形内腔的内壁上设有与形成于螺纹连接部152外侧的外螺纹相配合的内螺纹。
具体而言，第一漏斗部151的坡度范围30-60℃，优选45℃，第二漏斗部153的坡度范围30-60℃，优选45℃，可使样本能够完全集留在容纳瓶10底部。
作为一种优选的实施方式，螺纹连接部152靠近第一漏斗部151的一端沿径向方向延伸形成支撑部154。生物样本处理装置100还包括过滤片40。过滤片40位于下连接部15内并抵靠支撑部154。
作为一种优选的实施方式，下盖30的下端位于同一平面内，且该平面与容纳瓶10的中心轴线垂直。
具体而言，下盖30的下端位于同一平面内，且该平面与容纳瓶10的中心轴线垂直，以使得生物样本处理装置100可以通过下盖30稳定竖立于水平台面。
作为一种优选的实施方式，上盖组件20还包括上密封件25。上密封件25设置于上盖21形成的通孔的上端且与下密封件22配合以封闭上盖21围成的用于容纳与生物样本反应的溶液的腔。
具体而言，上密封件25和下密封件22将上盖21围成的用于容纳与生物样本反应的溶液的腔完全密封，使与生物样本反应的溶液与外界完全隔离。同时，上密封件25也可以防止活塞23被误按。
本生物样本处理装置100使用步骤如下：使用者旋开上盖21，收集生物样本于容纳瓶10内，旋紧上盖21。撕开上密封件25，推动活塞23，使活塞23下部十字形穿刺件24刺破下密封件22，与生物样本反应的溶液进入容纳瓶10与其内生物样本混合，在室温静置20～30min。将生物样本处理装置100置于离心机中，5000g室温离心15min。旋开下盖30，挤压弹性瓶体14，沉淀被过滤片40过滤、上清液从出液口12流出。
以上显示和描述了本发明的基本原理、主要特征和优点。本行业的技术人员应该了解，上述实施例不以任何形式限制本发明，凡采用等同替换或等效变换的方式所获得的技术方案，均落在本发明的保护范围内。

Claims (10)

  1. 一种生物样本处理装置，包括容纳瓶，用于盛放待处理的生物样本，所述容纳瓶的上端形成有接纳口且下端形成有出液口，其特征在于，所述生物样本处理装置还包括上盖组件和下盖；所述上盖组件连接至所述容纳瓶的形成有接纳口的上端；所述下盖连接至所述容纳瓶的形成有出液口的下端；所述上盖组件包括上盖、下密封件、活塞和穿刺件；所述上盖形成有通孔，所述下密封件设置于所述上盖形成的通孔的下端以使所述上盖形成一用于容纳与生物样本反应的溶液的腔；所述穿刺件连接至所述活塞；所述活塞与所述上盖形成的通孔的内壁紧密配合且可沿该内壁滑动以带动所述穿刺件刺破所述下密封件；所述上盖至少部分位于所述容纳瓶所形成的腔内。
  2. 根据权利要求1所述的生物样本处理装置，其特征在于，
    所述生物样本处理装置还包括过滤片；所述过滤片置于所述容纳瓶所形成的腔内以将所述容纳瓶所形成的腔分割成第一腔和第二腔。
  3. 根据权利要求1所述的生物样本处理装置，其特征在于，
    所述容纳瓶包括上连接部、可弹性形变的瓶体和下连接部；所述瓶体连接所述上连接部和所述下连接部；所述上连接部形成有接纳口；所述下连接部形成有出液口。
  4. 根据权利要求3所述的生物样本处理装置，其特征在于，
    所述上连接部外侧形成有外螺纹；所述上盖的上端一体形成有内壁设有内螺纹的套接部；所述套接部内壁设有与形成于所述上连接部外侧的外螺纹配合的内螺纹。
  5. 根据权利要求4所述的生物样本处理装置，其特征在于，
    所述上盖与所述瓶体之间形成有间隙。
  6. 根据权利要求3所述的生物样本处理装置，其特征在于，
    所述下连接部为漏斗形；所述出液口位于所述下连接部的尖端；所述下盖设有与所述下连接部相配合的漏斗形内腔。
  7. 根据权利要求6所述的生物样本处理装置，其特征在于，
    所述下连接部包括第一漏斗部、外侧形成有外螺纹的圆筒形的螺纹连接部和第二漏斗部；所述第一漏斗部连接至所述瓶体下端；所述螺纹连接部一端连接至所述第一漏斗部且另一端连接至所述第二漏斗部；所述出液口位于所述第二漏斗部的尖端；所述下盖的与所述下连接部相配合的漏斗形内腔的内壁上设有与形成于所述螺纹连接部外侧的外螺纹相配合的内螺纹。
  8. 根据权利要求7所述的生物样本处理装置，其特征在于，
    所述螺纹连接部靠近所述第一漏斗部的一端沿径向方向延伸形成支撑部；所述生物样本处理装置还包括过滤片；所述过滤片位于所述下连接部内并抵靠所述支撑部。
  9. 根据权利要求1所述的生物样本处理装置，其特征在于，
    所述下盖的下端位于同一平面内，且该平面与所述容纳瓶的中心轴线垂直。
  10. 根据权利要求1所述的生物样本处理装置，其特征在于，
    所述上盖组件还包括上密封件；所述上密封件设置于所述上盖形成的通孔的上端且与所述下密封件配合以封闭所述上盖围成的用于容纳与生物样本反应的溶液的腔。
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