WO2015185998A2 - Procédés de blocage de la voie de signalisation des cxcr-4/sdf-1 à l'aide d'inhibiteurs de kinase x de moelle osseuse - Google Patents

Procédés de blocage de la voie de signalisation des cxcr-4/sdf-1 à l'aide d'inhibiteurs de kinase x de moelle osseuse Download PDF

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WO2015185998A2
WO2015185998A2 PCT/IB2015/001425 IB2015001425W WO2015185998A2 WO 2015185998 A2 WO2015185998 A2 WO 2015185998A2 IB 2015001425 W IB2015001425 W IB 2015001425W WO 2015185998 A2 WO2015185998 A2 WO 2015185998A2
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alkyl
group
bmx
cxcr4
cancer
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PCT/IB2015/001425
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WO2015185998A3 (fr
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Raquel IZUMI
Francisco SALVA
Ahmed HAMDY
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Acerta Pharma B.V.
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the present invention relates to novel small molecule inhibitors that block the CXCR4-SDF-1 signaling pathway by directly inhibiting members of the Tec family of kinases, namely bone marrow X kinase (BMX) (also known as bone marrow tyrosine kinase gene in chromasone X and epithelial and endothelial tyrosine kinase (ETK)), and their use in treating diseases in which pathogenesis is mediated by the CXCR4/SDF-1 signaling pathway.
  • BMX bone marrow X kinase
  • ETK epithelial and endothelial tyrosine kinase
  • CXCR4 a G-protein-coupled receptor, and its naturally occurring ligand, stromal cell- derived factor- 1 (SDF-1; CXCL12), are a chemokine receptor- ligand pair.
  • CXCR4 is constitutive ly or over-expressed in a wide variety of human cancers (Table 1).
  • SDF-1 the only known ligand of CXCR4, is highly expressed in tumor microenvironments, as well as in bone marrow, lung, liver, and lymph nodes, i.e., organ sites most commonly involved in tumor metastasis.
  • CXCR4/SDF-1 interaction plays important roles in multiple stages of tumorigenesis, including tumor growth, invasion, angiogenesis, and metastasis (Furusato, et ah, Pathology International 2010, 60, 497-505).
  • the CXCR4/SDF1 axis also serves a role in attraction multiple leukocyte subsets and stimulation B cell production and myelopoeisis, all of which are implicated in autoimmune diseases (Chong and Mohan, Expert Opin. Ther. Targets 2009,
  • CXCR4/SDF-1 pathway may be an attractive therapeutic target (Tamamura and Fujii, Expert Opin. Ther. Targets 2005, 9(6), 1267-1282).
  • Tec kinases represent the second largest family of nonreceptor tyrosine kinases and are activated in response to cellular stimulation by antigen receptors, integrins, growth factors, cytokines and G protein-coupled receptors (Qiu and Kung, Oncogene 2000, 19, 5651-5661).
  • the mammalian Tec family consists of five members: Tec, BTK, Itk/Emt/Tsk, Rlk/Txk, and BMX/ETK (Mano, Cytokine Growth Factor Rev., 1999, 10, 267-280). With some exceptions, Tec kinases are expressed primarily in cells of hematopoietic lineages.
  • BMX also known as epithelial and endothelial tyrosine kinase (ETK)
  • ETK epithelial and endothelial tyrosine kinase
  • BMX contains an NH 2 -terminal Pleckstrin homology domain, a Src homology 3 domain, a Src homology 2 domain, and a COOH-terminal tyrosine kinase domain. Unlike other Tec-family kinases that are preferentially expressed in hematopoietic cells, BMX is expressed in other cell types as well, including endothelial, epithelial, and importantly metastatic carcinoma cells (Qiu, et al. , Proc. Natl. Acad. Sci. USA, 1998, 95, 3644-3649). BMX is activated by Gaq, Gal2 and Gal3. Thus, BMX plays an important role in G protein signaling and makes an attractive target for inhibition of CXCR4 signaling.
  • BMX inhibitors surprisingly block the CXCR4/SDF-1 signaling pathway. Accordingly, provided herein are methods of blocking the CXCR4/SDF-1 signaling in a subject in need thereof comprising administering to the subject a BMX inhibitor in an amount effective to block said CXCR4/SDF-1 signaling pathway. These methods may be used in the treatment of disorders associated with overexpression of CXCR4 or dysregulation of CXCR4 signaling, e.g., autoimmune disorders or hematologic or
  • the invention provides a BMX inhibitor capable of blocking the CXCR4/SDF-1 signaling pathway.
  • the present invention provides a pharmaceutical composition, comprising a BMX inhibitor or pharmaceutically acceptable salt thereof as variously described above, and a pharmaceutically acceptable carrier, diluent, or excipient.
  • the present invention provides a BMX inhibitor or pharmaceutically acceptable salt thereof as variously described above, for use in therapy.
  • the present invention provides a BMX inhibitor or pharmaceutically acceptable salt thereof as variously described above, for the treatment of autoimmune disorders or hematologic or nonhematologic malignancies.
  • the present invention provides the use of a BMX inhibitor or pharmaceutically acceptable salt thereof as variously described above, for the manufacture of a medicament for the treatment of autoimmune disorders or hematologic or nonhematologic malignancies.
  • the present invention provides a method of treating autoimmune disorders or hematologic or nonhematologic malignancies comprising administering to a patient in need thereof an effective amount of a BMX inhibitor or pharmaceutically acceptable salt thereof as variously described above.
  • FIG. 1 illustrates CD20 + B cell fluorescence, showing that CXCR-4/SDF-1 signalling is inhibited by the BMX inhibitor of Formula (II).
  • FIG. 2 illustrates CD14 + monocyte cell fluorescence, showing that CXCR-4/SDF-1 signalling is inhibited by the BMX inhibitor of Formula (II).
  • FIG. 3 illustrates CXCR4 surface expression in B-CLL (B cell chronic lymphocytic leukemia) following administration of the BMX inhibitor of Formula (II).
  • FIG. 4 illustrates CXCR4 surface expression in B-CLL following administration of the BMX inhibitor of Formula (II).
  • FIG. 5 illustrates that BMX inhibition is not impacting the ability of CD34 + cells to migrate towards SDF-1 (CXCL-12) in fresh CD34 + cells.
  • FIG. 6 illustrates that BMX inhibition is not impacting the ability of CD34 + cells to migrate towards SDF-1 (CXCL-12) in cryopreserved CD34 + cells.
  • FIG. 7 illustrates that BMX inhibition is not impacting the ability of U937 cells to migrate towards SDF-1 (CXCL-12).
  • FIG. 8 illustrates that BMX inhibition is not impacting the ability of CLL cells to migrate towards SDF-1 (CXCL-12).
  • FIG. 9 illustrates the results of a RANK MDA-MB231 mouse experiment.
  • SDF-1 includes two iso forms, SDF- ⁇ and SDF- ⁇ , currently understood to exhibit similar functionality, which are also called CXCL12.
  • Treatment refers to curative treatment of disorders associated CXCR4 receptor activity. Curative treatment refers to processes involving a slowing, interrupting, arresting, controlling, or stopping of disease progression, but does not necessarily involve a total elimination of all disease-related symptoms, conditions, or disorders.
  • co-administration encompass administration of two or more agents to a subject so that both agents and/or their metabolites are present in the subject at the same time.
  • Co-administration includes simultaneous administration in separate
  • compositions administration at different times in separate compositions, or administration in a composition in which two or more agents are present.
  • the term "effective amount” or “therapeutically effective amount” refers to that amount of a compound or combination of compounds as described herein that is sufficient to effect the intended application including, but not limited to, disease treatment.
  • a therapeutically effective amount may vary depending upon the intended application (in vitro or in vivo), or the subject and disease condition being treated (e.g., the weight, age and gender of the subject), the severity of the disease condition, the manner of administration, etc. which can readily be determined by one of ordinary skill in the art.
  • the term also applies to a dose that will induce a particular response in target cells, (e.g., the reduction of platelet adhesion and/or cell migration).
  • the specific dose will vary depending on the particular compounds chosen, the dosing regimen to be followed, whether the compound is administered in combination with other compounds, timing of administration, the tissue to which it is administered, and the physical delivery system in which the compound is carried.
  • a prophylactic effect includes delaying or eliminating the appearance of a disease or condition, delaying or eliminating the onset of symptoms of a disease or condition, slowing, halting, or reversing the progression of a disease or condition, or any combination thereof.
  • pharmaceutically acceptable salt refers to salts derived from a variety of organic and inorganic counter ions known in the art. Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and organic acids.
  • Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid and phosphoric acid.
  • Organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid and salicylic acid.
  • Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases.
  • Inorganic bases from which salts can be derived include, for example, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese and aluminum.
  • Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins. Specific examples include isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine.
  • the pharmaceutically acceptable base addition salt is chosen from ammonium, potassium, sodium, calcium, and magnesium salts.
  • cocrystal refers to a molecular complex derived from a number of cocrystal formers known in the art. Unlike a salt, a cocrystal typically does not involve hydrogen transfer between the cocrystal and the drug, and instead involves
  • intermolecular interactions such as hydrogen bonding, aromatic ring stacking, or dispersive forces, between the cocrystal former and the drug in the crystal structure.
  • “Pharmaceutically acceptable carrier” or “pharmaceutically acceptable excipient” is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions of the invention is contemplated. Supplementary active ingredients can also be incorporated into the described compositions.
  • Prodrug is intended to describe a compound that may be converted under
  • prodrug refers to a precursor of a biologically active compound that is pharmaceutically acceptable.
  • a prodrug may be inactive when administered to a subject, but is converted in vivo to an active compound, for example, by hydrolysis.
  • the prodrug compound often offers the advantages of solubility, tissue compatibility or delayed release in a mammalian organism (see, e.g., Bundgaard, H., Design of Prodrugs, Elsevier, Amsterdam, 1985).
  • prodrug is also intended to include any covalently bonded carriers, which release the active compound in vivo when administered to a subject.
  • Prodrugs of an active compound may be prepared by modifying functional groups present in the active compound in such a way that the modifications are cleaved, either in routine manipulation or in vivo, to yield the active parent compound.
  • Prodrugs include, for example, compounds wherein a hydroxy, amino or mercapto group is bonded to any group that, when the prodrug of the active compound is administered to a mammalian subject, cleaves to form a free hydroxy, free amino or free mercapto group, respectively.
  • prodrugs include, but are not limited to, acetates, formates and benzoate derivatives of an alcohol, various ester derivatives of a carboxylic acid, or acetamide, formamide and benzamide derivatives of an amine functional group in the active compound.
  • in vivo refers to an event that takes place in a subject's body.
  • in vitro refers to an event that takes places outside of a subject's body.
  • in vitro assays encompass cell-based assays in which cells alive or dead are employed and may also encompass a cell-free assay in which no intact cells are employed.
  • QD means quaque die, once a day, or once daily.
  • BID bis in die, twice a day, or twice daily.
  • TID means bis in die, twice a day, or twice daily.
  • TID means ter in die, three times a day, or three times daily.
  • QID means quater in die, four times a day, or four times daily.
  • Alkyl refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to ten carbon atoms (e.g., (Ci-io)alkyl or Cno alkyl).
  • a numerical range such as “1 to 10” refers to each integer in the given range - e.g., "1 to 10 carbon atoms” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms, although the definition is also intended to cover the occurrence of the term "alkyl” where no numerical range is specifically designated.
  • Typical alkyl groups include, but are in no way limited to, methyl, ethyl, propyl, isopropyl, n-butyl, iso-butyl, sec-butyl isobutyl, tertiary butyl, pentyl, isopentyl, neopentyl, hexyl, septyl, octyl, nonyl and decyl.
  • the alkyl moiety may be attached to the rest of the molecule by a single bond, such as for example, methyl (Me), ethyl (Et), n-propyl (Pr), 1-methylethyl (iso-propyl), n-butyl, n-pentyl, 1,1-dimethylethyl (t-butyl) and 3-methylhexyl.
  • an alkyl group is optionally substituted by one or more of substituents which are independently alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , - OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , - N(R a )C(0)R a , -N(R a )C(0)OR a , - N(R a )C(0)R
  • Alkylaryl refers to an -(alkyl)aryl radical where aryl and alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for aryl and alkyl respectively.
  • Alkylhetaryl refers to an -(alkyl)hetaryl radical where hetaryl and alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for aryl and alkyl respectively.
  • Alkylheterocycloalkyl refers to an -(alkyl) heterocycyl radical where alkyl and heterocycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heterocycloalkyl and alkyl respectively.
  • alkene refers to a group consisting of at least two carbon atoms and at least one carbon-carbon double bond
  • an "alkyne” moiety refers to a group consisting of at least two carbon atoms and at least one carbon-carbon triple bond.
  • the alkyl moiety, whether saturated or unsaturated, may be branched, straight chain, or cyclic.
  • alkenyl refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one double bond, and having from two to ten carbon atoms (i.e., (C 2 -io)alkenyl or C 2 - 10 alkenyl).
  • a numerical range such as “2 to 10” refers to each integer in the given range - e.g., "2 to 10 carbon atoms” means that the alkenyl group may consist of 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms.
  • the alkenyl moiety may be attached to the rest of the molecule by a single bond, such as for example, ethenyl (i.e., vinyl), prop-l-enyl (i.e., allyl), but-l-enyl, pent-l-enyl and penta-l,4-dienyl.
  • ethenyl i.e., vinyl
  • prop-l-enyl i.e., allyl
  • but-l-enyl but-l-enyl
  • pent-l-enyl and penta-l,4-dienyl.
  • an alkenyl group is optionally substituted by one or more substituents which are independently alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, - OR a , -SR a , -OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , - N(R a )C(0)OR a , -N(R a )C(0)R a , -N(R a )C(0)N(R a ) 2 , -N(R a
  • alkenyl-cycloalkyl refers to an -(alkenyl)cycloalkyl radical where alkenyl and cyclo alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for alkenyl and cycloalkyl respectively.
  • Alkynyl refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one triple bond, having from two to ten carbon atoms (i.e., (C 2 -io)alkynyl or C 2 - 10 alkynyl).
  • a numerical range such as “2 to 10” refers to each integer in the given range - e.g., "2 to 10 carbon atoms” means that the alkynyl group may consist of 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms.
  • alkynyl may be attached to the rest of the molecule by a single bond, for example, ethynyl, propynyl, butynyl, pentynyl and hexynyl.
  • an alkynyl group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano,
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl,
  • heterocycloalkylalkyl heteroaryl or heteroarylalkyl.
  • Alkynyl-cycloalkyl refers to an -(alkynyl)cycloalkyl radical where alkynyl and cycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for alkynyl and cycloalkyl respectively.
  • Cyano refers to a -CN radical.
  • Cycloalkyl refers to a monocyclic or polycyclic radical that contains only carbon and hydrogen, and may be saturated, or partially unsaturated. Cycloalkyl groups include groups having from 3 to 10 ring atoms (i.e. (C 3 -io)cycloalkyl or C 3 - 10 cycloalkyl). Whenever it appears herein, a numerical range such as “3 to 10" refers to each integer in the given range - e.g., "3 to 10 carbon atoms” means that the cycloalkyl group may consist of 3 carbon atoms, etc., up to and including 10 carbon atoms.
  • cycloalkyl groups include, but are not limited to the following moieties: cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloseptyl, cyclooctyl, cyclononyl, cyclodecyl, norbornyl, and the like.
  • a cycloalkyl group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , -OC(0)-R a , - N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , -N(R a )C(0)R a , -N(R a )C(0)OR a , -N(R a )
  • heterocycloalkylalkyl heteroaryl or heteroarylalkyl.
  • Cycloalkyl-alkenyl refers to a -(cycloalkyl)alkenyl radical where cycloalkyl and alkenyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for cycloalkyl and alkenyl, respectively.
  • Cycloalkyl-heterocycloalkyl refers to a -(cycloalkyl)heterocycloalkyl radical where cycloalkyl and heterocycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for cycloalkyl and
  • Cycloalkyl-heteroaryl refers to a -(cycloalkyl)heteroaryl radical where cycloalkyl and heteroaryl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for cycloalkyl and heteroaryl, respectively.
  • alkoxy refers to the group -O-alkyl, including from 1 to 8 carbon atoms of a straight, branched, cyclic configuration and combinations thereof attached to the parent structure through an oxygen. Examples include, but are not limited to, methoxy, ethoxy, propoxy, isopropoxy, cyclopropyloxy and cyclohexyloxy. "Lower alkoxy” refers to alkoxy groups containing one to six carbons.
  • substituted alkoxy refers to alkoxy wherein the alkyl constituent is substituted (i.e., -0-(substituted alkyl)).
  • the alkyl moiety of an alkoxy group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , -OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , - C(0)N(R a ) 2 , - C(0)N(R a )
  • a (Ci- 6 )alkoxy carbonyl group is an alkoxy group having from 1 to 6 carbon atoms attached through its oxygen to a carbonyl linker.
  • Lower alkoxycarbonyl refers to an alkoxycarbonyl group wherein the alkoxy group is a lower alkoxy group.
  • substituted alkoxycarbonyl refers to the group (substituted alkyl)-O-C(O)- wherein the group is attached to the parent structure through the carbonyl functionality.
  • the alkyl moiety of an alkoxycarbonyl group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , - OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a )
  • Acyl refers to the groups (alkyl)-C(O)-, (aryl)-C(O)-, (heteroaryl)-C(O)-,
  • heteroalkyl C(O)- and (heterocycloalkyl)-C(O)-, wherein the group is attached to the parent structure through the carbonyl functionality.
  • R radical is heteroaryl or heterocycloalkyl, the hetero ring or chain atoms contribute to the total number of chain or ring atoms.
  • the alkyl, aryl or heteroaryl moiety of the acyl group is optionally substituted by one or more substituents which are independently alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , - OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , - N(R a )C(0)R a , -N(R a )C(0)OR a
  • R of an acyloxy group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , - OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , - N(R a )C(0)R a , -N(R a )C(0)OR a , - N(R a )
  • Amino refers to a -N(R a ) 2 radical group, where each R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl, unless stated otherwise specifically in the specification.
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl, unless stated otherwise specifically in the specification.
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroaryl
  • -N(R a ) 2 is intended to include, but is not limited to, 1-pyrrolidinyl and 4-morpholinyl.
  • an amino group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano,
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl,
  • substituted amino also refers to N-oxides of the groups -NHR d , and NR d R d each as described above. N-oxides can be prepared by treatment of the corresponding amino group with, for example, hydrogen peroxide or m-chloroperoxybenzoic acid.
  • Amide or “amido” refers to a chemical moiety with formula -C(0)N(R) 2 or
  • R is selected from the group consisting of hydrogen, alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon), each of which moiety may itself be optionally substituted.
  • the R 2 of -N(R) 2 of the amide may optionally be taken together with the nitrogen to which it is attached to form a 4-, 5-, 6- or 7- membered ring.
  • an amido group is optionally substituted independently by one or more of the substituents as described herein for alkyl, cycloalkyl, aryl, heteroaryl, or heterocycloalkyl.
  • An amide may be an amino acid or a peptide molecule attached to a compound disclosed herein, thereby forming a prodrug.
  • the procedures and specific groups to make such amides are known to those of skill in the art and can readily be found in seminal sources such as Greene and Wuts, Protective Groups in Organic Synthesis, 3 rd Ed., John Wiley & Sons, New York, 1999, which is incorporated herein by reference in its entirety.
  • Aromatic or "aryl” or “Ar” refers to an aromatic radical with six to ten ring atoms (e.g., C 6 -Cio aromatic or C 6 -Ci 0 aryl) which has at least one ring having a conjugated pi electron system which is carbocyclic (e.g., phenyl, fluorenyl, and naphthyl).
  • Bivalent radicals formed from substituted benzene derivatives and having the free valences at ring atoms are named as substituted phenylene radicals.
  • Bivalent radicals derived from univalent poly eye lie hydrocarbon radicals whose names end in "-yl” by removal of one hydrogen atom from the carbon atom with the free valence are named by adding "-idene” to the name of the corresponding univalent radical, e.g., a naphthyl group with two points of attachment is termed naphthylidene.
  • a numerical range such as “6 to 10” refers to each integer in the given range; e.g., "6 to 10 ring atoms” means that the aryl group may consist of 6 ring atoms, 7 ring atoms, etc., up to and including 10 ring atoms.
  • an aryl moiety is optionally substituted by one or more substituents which are independently alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, -OR a , -SR a , -OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , -N(R a ) 2 , -N(R a )C(0)OR a , -N(R a )C(0)OR a ,
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or
  • alkyl refers to an (aryl)alkyl-radical where aryl and alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for aryl and alkyl respectively.
  • Ester refers to a chemical radical of formula -COOR, where R is selected from the group consisting of alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon).
  • R is selected from the group consisting of alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon).
  • an ester group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano,
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl,
  • heterocycloalkylalkyl heteroaryl or heteroarylalkyl.
  • Fluoroalkyl refers to an alkyl radical, as defined above, that is substituted by one or more fluoro radicals, as defined above, for example, trifluoromethyl, difluoromethyl, 2,2,2- trifluoroethyl, l-fluoromethyl-2-fluoroethyl, and the like.
  • the alkyl part of the fluoroalkyl radical may be optionally substituted as defined above for an alkyl group.
  • Halo is intended to mean fluoro, chloro, bromo or iodo.
  • haloalkyl haloalkenyl
  • haloalkynyl haloalkoxy
  • Heteroalkyl “heteroalkenyl” and “heteroalkynyl” include optionally substituted alkyl, alkenyl and alkynyl radicals and which have one or more skeletal chain atoms selected from an atom other than carbon, e.g., oxygen, nitrogen, sulfur, phosphorus or combinations thereof.
  • a numerical range may be given - e.g., C1-C4 heteroalkyl which refers to the chain length in total, which in this example is 4 atoms long.
  • a heteroalkyl group may be substituted with one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, nitro, oxo, thioxo, trimethylsilanyl, -OR a , -SR a , -OC(0)-R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , - OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , -N(R a )C(0)R a , -N(R a )C(0)OR a , -N(R a )C(0)R a
  • R a is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl.
  • Heteroalkylaryl refers to an -(heteroalkyl)aryl radical where heteroalkyl and aryl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and aryl, respectively.
  • Heteroalkylheteroaryl refers to an -(heteroalkyl)heteroaryl radical where heteroalkyl and heteroaryl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and heteroaryl, respectively.
  • Heteroalkylheterocycloalkyl refers to an -(heteroalkyl)heterocycloalkyl radical where heteroalkyl and heterocycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and
  • Heteroalkylcycloalkyl refers to an -(heteroalkyl)cycloalkyl radical where heteroalkyl and cycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and cycloalkyl, respectively.
  • Heteroaryl or “heteroaromatic” or “HetAr” refers to a 5- to 18-membered aromatic radical (e.g., C5-C13 heteroaryl) that includes one or more ring heteroatoms selected from nitrogen, oxygen and sulfur, and which may be a monocyclic, bicyclic, tricyclic or tetracyclic ring system.
  • a numerical range such as “5 to 18” refers to each integer in the given range - e.g., "5 to 18 ring atoms” means that the heteroaryl group may consist of 5 ring atoms, 6 ring atoms, etc., up to and including 18 ring atoms.
  • Bivalent radicals derived from univalent heteroaryl radicals whose names end in "-yl” by removal of one hydrogen atom from the atom with the free valence are named by adding "-idene” to the name of the corresponding univalent radical - e.g., a pyridyl group with two points of attachment is a pyridylidene.
  • a N-containing "heteroaromatic” or “heteroaryl” moiety refers to an aromatic group in which at least one of the skeletal atoms of the ring is a nitrogen atom.
  • the polycyclic heteroaryl group may be fused or non-fused.
  • the heteroatom(s) in the heteroaryl radical are optionally oxidized.
  • heteroaryl may be attached to the rest of the molecule through any atom of the ring(s).
  • heteroaryls include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzindolyl, 1,3-benzodioxolyl, benzofuranyl, benzooxazolyl, benzo[ ⁇ i]thiazolyl, benzothiadiazolyl, benzo[£][l,4]dioxepinyl, benzo[£][l,4]oxazinyl, 1 ,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzoxazolyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranon
  • a heteroaryl moiety is optionally substituted by one or more substituents which are independently: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, nitro, oxo, thioxo, trimethylsilanyl, -OR a , -SR a , -OC(O)- R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , -N(R a )C(0)R a , -N(R a )C(0)OR a , -N(R a )C(0)
  • heterocycloalkylalkyl heteroaryl or heteroarylalkyl.
  • Substituted heteroaryl also includes ring systems substituted with one or more oxide (-0-) substituents, such as, for example, pyridinyl N-oxides.
  • Heteroarylalkyl refers to a moiety having an aryl moiety, as described herein, connected to an alkylene moiety, as described herein, wherein the connection to the remainder of the molecule is through the alkylene group.
  • Heterocycloalkyl refers to a stable 3- to 18-membered non-aromatic ring radical that comprises two to twelve carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. Whenever it appears herein, a numerical range such as “3 to 18" refers to each integer in the given range - e.g., "3 to 18 ring atoms” means that the heterocycloalkyl group may consist of 3 ring atoms, 4 ring atoms, etc., up to and including 18 ring atoms.
  • the heterocycloalkyl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which may include fused or bridged ring systems.
  • the heteroatoms in the heterocycloalkyl radical may be optionally oxidized.
  • One or more nitrogen atoms, if present, are optionally quaternized.
  • the heterocycloalkyl radical is partially or fully saturated.
  • the heterocycloalkyl may be attached to the rest of the molecule through any atom of the ring(s).
  • heterocycloalkyl radicals include, but are not limited to, dioxolanyl, thienyl[l,3]dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2- oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4- piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl, thiomorpholinyl, thiamorpholinyl, 1-oxox
  • a heterocycloalkyl moiety is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, nitro, oxo, thioxo, trimethylsilanyl, -OR a , -SR a , -OC(O)- R a , -N(R a ) 2 , -C(0)R a , -C(0)OR a , -OC(0)N(R a ) 2 , -C(0)N(R a ) 2 , -N(R a )C(0)OR a , -N(R a )C(0)R a , -N(R a )C(0)OR a , -N(R a
  • heterocycloalkylalkyl heteroaryl or heteroarylalkyl.
  • Heterocycloalkyl also includes bicyclic ring systems wherein one non-aromatic ring, usually with 3 to 7 ring atoms, contains at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms; and the other ring, usually with 3 to 7 ring atoms, optionally contains 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen and is not aromatic.
  • Niro refers to the -N0 2 radical.
  • Oxa refers to the -O- radical.
  • Stepoisomers are isomers that differ only in the way the atoms are arranged in space - i.e., having a different stereochemical configuration.
  • Enantiomers are a pair of stereoisomers that are non-superimposable mirror images of each other.
  • a 1 : 1 mixture of a pair of enantiomers is a “racemic” mixture.
  • the term “( ⁇ )” is used to designate a racemic mixture where appropriate.
  • “Diastereoisomers” are stereoisomers that have at least two asymmetric atoms, but which are not mirror-images of each other. The absolute stereochemistry is specified according to the Cahn- Ingold-Prelog R-S system.
  • stereochemistry at each chiral carbon can be specified by either (R) or (S).
  • Resolved compounds whose absolute configuration is unknown can be designated (+) or (-) depending on the direction (dextro- or levorotatory) which they rotate plane polarized light at the wavelength of the sodium D line.
  • Certain of the compounds described herein contain one or more asymmetric centers and can thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that can be defined, in terms of absolute stereochemistry, as (R) or (S).
  • the present chemical entities, pharmaceutical compositions and methods are meant to include all such possible isomers, including racemic mixtures, optically pure forms and intermediate mixtures.
  • Optically active (R)- and (5)-isomers can be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques.
  • the compounds described herein contain olefmic double bonds or other centers of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers.
  • Enantiomeric purity refers to the relative amounts, expressed as a percentage, of the presence of a specific enantiomer relative to the other enantiomer. For example, if a compound, which may potentially have an (R)- or an (5)-isomeric configuration, is present as a racemic mixture, the enantiomeric purity is about 50% with respect to either the (R)- or (iS)-isomer. If that compound has one isomeric form predominant over the other, for example, 80% (iS)-isomer and 20%> (i?)-isomer, the enantiomeric purity of the compound with respect to the (S)-isomeric form is 80%.
  • the enantiomeric purity of a compound can be determined in a number of ways known in the art, including but not limited to chromatography using a chiral support, polarimetric measurement of the rotation of polarized light, nuclear magnetic resonance spectroscopy using chiral shift reagents which include but are not limited to lanthanide containing chiral complexes or Pirkle's reagents, or derivatization of a compounds using a chiral compound such as Mosher's acid followed by chromatography or nuclear magnetic resonance spectroscopy.
  • the enantiomerically enriched composition has a higher potency with respect to therapeutic utility per unit mass than does the racemic mixture of that composition.
  • Enantiomers can be isolated from mixtures by methods known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts; or preferred enantiomers can be prepared by asymmetric syntheses.
  • an enantiomerically enriched preparation of the (S)-enantiomer means a preparation of the compound having greater than 50% by weight of the (5)-enantiomer relative to the ( ?)-enantiomer, such as at least 75% by weight, or such as at least 80% by weight.
  • the enrichment can be significantly greater than 80% by weight, providing a "substantially enantiomerically enriched” or a “substantially non-racemic” preparation, which refers to preparations of compositions which have at least 85% by weight of one enantiomer relative to other enantiomer, such as at least 90%> by weight, or such as at least 95% by weight.
  • enantiomerically pure or “substantially enantiomerically pure” refers to a composition that comprises at least 98% of a single enantiomer and less than 2% of the opposite enantiomer.
  • Moiety refers to a specific segment or functional group of a molecule. Chemical moieties are often recognized chemical entities embedded in or appended to a molecule.
  • Tautomers are structurally distinct isomers that interconvert by tautomerization.
  • Tautomerization is a form of isomerization and includes prototropic or proton-shift tautomerization, which is considered a subset of acid-base chemistry. "Prototropic
  • tautomerization e.g. in solution
  • keto-enol tautomerization A specific example of keto-enol tautomerization is the interconversion of pentane-2,4-dione and 4- hydroxypent-3-en-2-one tautomers.
  • phenol-keto tautomerization A specific example of phenol-keto tautomerization is the interconversion of pyridin-4-ol and pyridin-4(lH)-one tautomers.
  • a "leaving group or atom” is any group or atom that will, under selected reaction conditions, cleave from the starting material, thus promoting reaction at a specified site.
  • Examples of such groups include halogen atoms and mesyloxy, p- nitrobenzensulphonyloxy and tosyloxy groups.
  • Protecting group is intended to mean a group that selectively blocks one or more reactive sites in a multifunctional compound such that a chemical reaction can be carried out selectively on another unprotected reactive site and the group can then be readily removed after the selective reaction is complete.
  • a variety of protecting groups are disclosed, for example, in T. H. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, Third Edition, John Wiley & Sons, New York (1999).
  • Solvate refers to a compound in physical association with one or more molecules of a pharmaceutically acceptable solvent.
  • Substituted means that the referenced group may have attached one or more additional groups, radicals or moieties individually and independently selected from, for example, acyl, alkyl, alkylaryl, cycloalkyl, aralkyl, aryl, carbohydrate, carbonate, heteroaryl, heterocycloalkyl, hydroxy, alkoxy, aryloxy, mercapto, alkylthio, arylthio, cyano, halo, carbonyl, ester, thiocarbonyl, isocyanato, thiocyanato, isothiocyanato, nitro, oxo, perhaloalkyl,
  • perfluoroalkyl perfluoroalkyl, phosphate, silyl, sulfmyl, sulfonyl, sulfonamidyl, sulfoxyl, sulfonate, urea, and amino, including mono- and di-substituted amino groups, and protected derivatives thereof.
  • the substituents themselves may be substituted, for example, a cycloalkyl substituent may itself have a halide substituent at one or more of its ring carbons.
  • optionally substituted means optional substitution with the specified groups, radicals or moieties.
  • Sulfanyl refers to groups that include -S-(optionally substituted alkyl), -S -(optionally substituted aryl), -S-(optionally substituted heteroaryl) and -S -(optionally substituted
  • Sulfmyl refers to groups that include -S(0)-H, -S(0)-(optionally substituted alkyl), -S(0)-(optionally substituted amino), -S(0)-(optionally substituted aryl), -S(0)-(optionally substituted heteroaryl) and -S(0)-(optionally substituted heterocycloalkyl).
  • Sulfonyl refers to groups that include -S(0 2 )-H, -S(0 2 )-(optionally substituted alkyl), -S(0 2 )-(optionally substituted amino), -S(0 2 )-(optionally substituted aryl), -S(0 2 )-(optionally substituted heteroaryl), and -S(0 2 )-(optionally substituted heterocycloalkyl).
  • a sulfonamido group is optionally
  • a sulfonate group is optionally substituted on R by one or more of the substituents described for alkyl, cycloalkyl, aryl, heteroaryl, respectively.
  • Compounds of the invention also include crystalline and amorphous forms of those compounds, including, for example, polymorphs, pseudopolymorphs, solvates, hydrates, unsolvated polymorphs (including anhydrates), conformational polymorphs, and amorphous forms of the compounds, as well as mixtures thereof.
  • Crystalstalline form and polymorph are intended to include all crystalline and amorphous forms of the compound, including, for example, polymorphs, pseudopolymorphs, solvates, hydrates, unsolvated polymorphs (including anhydrates), conformational polymorphs, and amorphous forms, as well as mixtures thereof, unless a particular crystalline or amorphous form is referred to.
  • Inhibitors of BMX kinase activity include compounds which inhibits BMX activity with an IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar in an ADP-GLOTM, bioluminescent, homogeneous assay or in a HTRF (Homogeneous Time-Resolved Fluorescence) assay.
  • IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar in an ADP-GLOTM, bioluminescent, homogeneous assay or in a HTRF (Homogeneous Time-Resolved Fluorescence) assay.
  • IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than
  • Inhibitors of BMX kinase activity may inhibit phosphorylation of Y40 of BMX with an IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar.
  • Inhibitors of BMX kinase activity may inhibit phosphorylation of Y215 of BMX with an IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar.
  • Inhibitors of BMX kinase activity may inhibit phosphorylation of Y223 of BMX with an IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar.
  • Inhibitors of BMX kinase activity may inhibit phosphorylation of Y566 of BMX with an IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar.
  • Inhibitors of BMX kinase activity may inhibit phosphorylation of downstream PAK1 with an IC 50 of less than or equal to 10 micromolar, less than or equal to 1 micromolar, less than or equal to 500 nanomolar, less than or equal to 100 nanomolar, or less than or equal to 10 nanomolar in, e.g., a phospho-PAK assay.
  • the BMX inhibitor is a compound of Formula (I):
  • X is CH, N, O or S
  • Y is C(R 6 ), N, O or S;
  • Z is CH, N or bond
  • A is CH or N
  • Bi is N or C(R 7 );
  • B 2 is N or C(R 8 );
  • B 3 is N or C(R 9 );
  • R 2 is H, (Ci_ 3 )alkyl or (C 3 _ 7 )cycloalkyl;
  • R 3 is H, (Ci_ 6 )alkyl or (C 3 _ 7 )cycloalkyl); or
  • R 2 and R 3 form, together with the N and C atom they are attached to, a (C 3 _ 7 )heterocycloalkyl optionally substituted with one or more fluorine, hydroxyl, (Ci_ 3 )alkyl, (Ci_ 3 )alkoxy or oxo;
  • R4 is H or (Ci_ 3 )alkyl;
  • R 5 is H, halogen, cyano, (Ci_ 4 )alkyl, (Ci_ 3 )alkoxy, (C 3 _ 6 )cycloalkyl, any alkyl group of which is optionally substituted with one or more halogen; or R 5 is (C 6 -io)aryl or (C 2- 6 )heterocycloalkyl;
  • Re is H or (Ci_ 3 )alkyl
  • R 5 and R 6 together may form a (C 3 _7)cycloalkenyl or (C2_6)heterocycloalkenyl, each optionally substituted with (Ci_ 3 )alkyl or one or more halogens;
  • R 7 is H, halogen, CF 3 , (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy;
  • Rg is H, halogen, CF 3 , (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy; or
  • R9 is H, halogen, (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy;
  • Rio is H, halogen, (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy;
  • R11 is independently selected from the group consisting of (Ci_ 6 )alkyl, (C 2 _6)alkenyl and (C 2- 6 ) alkynyl, where each alkyl, alkenyl or alkynyl is optionally substituted with one or more substituents selected from the group consisting of hydroxyl, (Ci_ 4 )alkyl, (C 3 _7)cycloalkyl, [(Ci_ 4 )alkyl]amino, di[(Ci_ 4 )alkyl]amino, (Ci_ 3 )alkoxy, (C 3 _7)cycloalkoxy, (C 6 -io)aryl and (C 3 _
  • Rn is (Ci_ 3 )alkyl-C(0)-S-(Ci_ 3 )alkyl; or
  • R11 is (Ci_ 5 )heteroaryl optionally substituted with one or more substituents selected from the group consisting of halogen or cyano;
  • R12 and Ri 3 are independently selected from the group consisting of (C 2 _6)alkenyl or (C 2- 6 )alkynyl, both optionally substituted with one or more substituents selected from the group consisting of hydroxyl, (Ci_ 4 )alkyl, (C 3 _ 7 )cycloalkyl, [(Ci_ 4 )alkyl]amino, di[(Ci_ 4 )alkyl]amino, (Ci_ 3 )alkoxy, (C 3 _7)cycloalkoxy, (C 6 -io)aryl and (C 3 _7)heterocycloalkyl; or a (Ci_5)heteroaryl optionally substituted with one or more substituents selected from the group consisting of halogen and cyano; and
  • Ri 4 is independently selected from the group consisting of halogen, cyano, (C 2 _6)alkenyl and (C 2- 6 )alkynyl, both optionally substituted with one or more substituents selected from the group consisting of hydroxyl, (Ci_4)alkyl, (C 3 _ 7 )cycloalkyl, (Ci_4)alkylamino, di[(Ci_4)alkyl]amino, (Ci_ 3 )alkoxy, (C 3 _ 7 )cycloalkoxy, (C 6 _io)aryl, (Ci_ 5 )heteroaryl and (C 3 _ 7 )heterocycloalkyl; with the proviso that:
  • X, Y, Z can simultaneously be a heteroatom; when one atom selected from X, Y is O or S, then Z is a bond and the other atom selected from X, Y can not be O or S; when Z is C or N then Y is C(R 6 ) or N and X is C or N;
  • B ls B 2 , B 3 and B 4 are N; with the terms used having the following meanings:
  • (Ci_ 2 )alkyl means an alkyl group having 1 to 2 carbon atoms, being methyl or ethyl,
  • (Ci_ 3 )alkyl means a branched or unbranched alkyl group having 1-3 carbon atoms, being methyl, ethyl, propyl or isopropyl;
  • (Ci_ 4 )alkyl means a branched or unbranched alkyl group having 1-4 carbon atoms, being methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl and tert-butyl, (Ci_ 3 )alkyl groups being preferred;
  • (Ci_5)alkyl means a branched or unbranched alkyl group having 1-5 carbon atoms, for example methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl and isopentyl, (Ci_ 4 )alkyl groups being preferred.
  • (Ci_ 6 )Alkyl means a branched or unbranched alkyl group having 1-6 carbon atoms, for example methyl, ethyl, propyl, isopropyl, butyl, tert-butyl, n- pentyl and n-hexyl.
  • (Ci_ 5 )alkyl groups are preferred, (Ci_ 4 )alkyl being most preferred;
  • (Ci_ 2 )alkoxy means an alkoxy group having 1-2 carbon atoms, the alkyl moiety having the same meaning as previously defined;
  • (Ci_ 3 )alkoxy means an alkoxy group having 1-3 carbon atoms, the alkyl moiety having the same meaning as previously defined. (Ci_ 2 )alkoxy groups are preferred;
  • (Ci_ 4 )alkoxy means an alkoxy group having 1-4 carbon atoms, the alkyl moiety having the same meaning as previously defined.
  • (Ci_ 3 )alkoxy groups are preferred,
  • (Ci_ 2 )alkoxy groups being most preferred;
  • (C 2 -4)alkenyl means a branched or unbranched alkenyl group having 2-4 carbon atoms, such as ethenyl, 2-propenyl, isobutenyl or 2-butenyl;
  • (C 2 _ 6 )alkenyl means a branched or unbranched alkenyl group having 2-6 carbon atoms, such as ethenyl, 2-butenyl, and n-pentenyl, (C 2 _4)alkenyl groups being most preferred;
  • (C 2 _4)alkynyl means a branched or unbranched alkynyl group having 2-4 carbon atoms, such as ethynyl, 2-propynyl or 2-butynyl;
  • (C 2 _ 6 )alkynyl means a branched or unbranched alkynyl group having 2-6 carbon atoms, such as ethynyl, propynyl, n-butynyl, n-pentynyl, isopentynyl, isohexynyl or n-hexynyl.
  • (C 2 _4)alkynyl groups are preferred;
  • (C 3 _6)cycloalkyl means a cycloalkyl group having 3-6 carbon atoms, being cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl;
  • (C 3 _7)cycloalkyl means a cycloalkyl group having 3-7 carbon atoms, being cyclopropyl
  • (C 2 _6)heterocycloalkyl means a heterocycloalkyl group having 2-6 carbon atoms, preferably 3-5 carbon atoms, and one or two heteroatoms selected from N, O and/or S, which may be attached via a heteroatom if feasible, or a carbon atom; preferred heteroatoms are N or O; also preferred are piperidine, morpholine, pyrrolidine and piperazine; with the most preferred (C 2 _6)heterocycloalkyl being pyrrolidine; the heterocycloalkyl group may be attached via a heteroatom if feasible;
  • heterocycloalkyl means a heterocycloalkyl group having 3-7 carbon atoms, preferably 3-5 carbon atoms, and one or two heteroatoms selected from N, O and/or S. Preferred
  • heteroatoms are N or O; preferred (C 3-7 ) heterocycloalkyl groups are azetidinyl, pyrrolidinyl, piperidinyl, homopiperidinyl or morpholinyl; more preferred (C 3 _7)heterocycloalkyl groups are piperidine, morpholine and pyrrolidine; and the heterocycloalkyl group may be attached via a heteroatom if feasible;
  • (C3_7)cycloalkoxy means a cycloalkyl group having 3-7 carbon atoms, with the same meaning as previously defined, attached via a ring carbon atom to an exocyclic oxygen atom;
  • (C 6 -io)aryl means an aromatic hydrocarbon group having 6-10 carbon atoms, such as phenyl, naphthyl, tetrahydronaphthyl or indenyl; the preferred (C 6 -io)aryl group is phenyl;
  • (Ci_5)heteroaryl means a substituted or unsubstituted aromatic group having 1-5 carbon atoms and 1-4 heteroatoms selected from N, O and/or S; the (Ci_5)heteroaryl may optionally be substituted; preferred (Ci_ 5 )heteroaryl groups are tetrazolyl, imidazolyl, thiadiazolyl, pyridyl, pyrimidyl, triazinyl, thienyl or furyl, a more preferred (Ci_5)heteroaryl is pyrimidyl;
  • (Ci_9)heteroaryl means a substituted or unsubstituted aromatic group having 1-9 carbon atoms and 1-4 heteroatoms selected from N, O and/or S; the (Ci_9)heteroaryl may optionally be substituted; preferred (Ci_ 9 )heteroaryl groups are quinoline, isoquinoline and indole;
  • [(Ci_4)alkyl]amino means an amino group, monosubstituted with an alkyl group containing 1-4 carbon atoms having the same meaning as previously defined; preferred [(Ci_4)alkyl]amino group is methylamino; di[(Ci_ 4 )alkyl]amino means an amino group, disubstituted with alkyl group(s), each containing 1- 4 carbon atoms and having the same meaning as previously defined; preferred di[(Ci_4)
  • 4)alkyl] amino group is dimethylamino; halogen means means fluorine, chlorine, bromine or iodine;
  • (Ci_3)alkyl-C(0)-S-(Ci_3)alkyl means an alkyl-carbonyl-thio-alkyl group, each of the alkyl
  • (C3_7)cycloalkenyl means a cycloalkenyl group having 3-7 carbon atoms, preferably 5-7 carbon atoms; preferred (C3_ 7 )cycloalkenyl groups are cyclopentenyl or cyclohexenyl; cyclohexenyl groups are most preferred;
  • (C2_6)heterocycloalkenyl means a heterocycloalkenyl group having 2-6 carbon atoms, preferably 3-5 carbon atoms; and 1 heteroatom selected from N, O and/or S; preferred (C 2- 6)heterocycloalkenyl groups are oxycyclohexenyl and azacyclohexenyl group.
  • the attachment point is at the last group.
  • substituents are optionally substituted, this also includes the alkyl moiety of an alkoxy group.
  • a circle in a ring of Formula (I) indicates that the ring is aromatic.
  • the nitrogen if present in X or Y, may carry a hydrogen.
  • Bi is C(R 7 ); B 2 is C(R 8 ); B 3 is C(R 9 ); B 4 is C(R W ); R 7 , R9, and Rio are each H; and Rg is hydrogen or methyl.
  • the ring containing X, Y and Z is selected from the group consisting of pyridyl, pyrimidyl, pyridazyl, triazinyl, thiazolyl, oxazolyl and isoxazolyl.
  • the ring containing X, Y and Z is selected from the group consisting of pyridyl, pyrimidyl and pyridazyl.
  • the ring containing X, Y and Z is selected from the group consisting of pyridyl and pyrimidyl.
  • the ring containing X, Y and Z is pyridyl.
  • R 5 is selected from the group consisting of hydrogen, fluorine, methyl, methoxy and trifluoromethyl.
  • R 5 is hydrogen
  • R 2 and R 3 together form a heterocycloalkyl ring selected from the group consisting of azetidinyl, pyrrolidinyl, piperidinyl, homopiperidinyl and morpholinyl, optionally substituted with one or more of fluoro, hydroxyl, (Ci_ 3 )alkyl and (Ci_ 3 )alkoxy.
  • R 2 and R 3 together form a heterocycloalkyl ring selected from the group consisting of azetidinyl, pyrrolidinyl and piperidinyl.
  • R 2 and R 3 together form a pyrrolidinyl ring.
  • Ri is independently selected from the group consisting of (Ci_ 6 )alkyl, (C 2 _6)alkenyl or (C 2 _6)alkynyl, each optionally substituted with one or more substituents selected from the group consisting of hydroxyl, (Ci_4)alkyl, (C 3 _ 7 )cycloalkyl, [(Ci_4)alkyl]amino, di[(Ci_4)alkyl] amino, (Ci_ 3 )alkoxy, (C 3 _ 7 )cycloalkoxy, (C 6 -io)aryl and (C 3 _ 7 )heterocycloalkyl.
  • B ls B 2 , B 3 and B 4 are CH; X is N; Y and Z are CH; R 5 is CH 3 ; A is N; R 2 , R 3 and R 4 are H; and Ri is CO-CH 3 .
  • B ls B 2 , B 3 and B4 are CH; X and Y are N; Z is CH; R5 is CH 3 ; A is N; R 2 , R 3 and R 4 are H; and Ri is CO-CH 3 .
  • B ls B 2 , B 3 and B 4 are CH; X and Y are N; Z is CH; R 5 is CH 3 ; A is CH; R 2 and R 3 together form a piperidinyl ring; R4 is H; and Ri is CO-ethenyl.
  • B ls B 2 , B 3 and B 4 are CH; X, Y and Z are CH; R5 is H; A is CH; R 2 and R 3 together form a pyrrolidinyl ring; R4 is H; and Ri is CO-propynyl.
  • B ls B 2 , B 3 and B 4 are CH; X, Y and Z are CH; R5 is CH 3 ; A is CH; R 2 and R 3 together form a piperidinyl ring; R 4 is H; and Ri is CO-propynyl.
  • B ls B 2 , B 3 and B 4 are CH; X and Y are N; Z is CH; R5 is H; A is CH; R 2 and R 3 together form a morpholinyl ring; R4 is H; and Ri is CO-ethenyl.
  • B ls B 2 , B 3 and B 4 are CH; X and Y are N; Z is CH; R5 is CH 3 ; A is CH; R 2 and R 3 together form a morpholinyl ring; R 4 is H; and Ri is CO-propynyl.
  • the BMX inhibitor is a compound of Formula (II):
  • the BMX inhibitor is (5)-4-(8-amino-3-(l-(but-2-ynoyl)pyrrolidin- 2-yl)imidazo[l ,5-a]pyrazin-l-yl)-N-(pyridin-2-yl)benzamide or pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug therof.
  • the BMX inhibitors include, but are not limited to, those compounds described in International Patent Application Publication No. WO 2013/010868, the disclosures of each of which are specifically incorporated by reference herein.
  • the BMX inhibitor is a compound of Formula (III) or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof:
  • X is CH, N, O or S
  • Y is C(R 6 ), N, O or S;
  • Z is CH, N or bond
  • A is CH or N
  • Bi is N or C(R 7 );
  • B 2 is N or C(R 8 );
  • B 3 is N or C(R 9 );
  • Ri is RiiC(O), Ri 2 S(0), R 13 SO 2 or (Ci_ 6 )alkyl optionally substituted with R i4 ;
  • R 2 is H, (Ci_ 3 )alkyl or (C 3 _ 7 )cycloalkyl;
  • R 3 is H, (Ci_ 6 )alkyl or (C 3 _ 7 )cycloalkyl); or
  • R 2 and R 3 form, together with the N and C atom they are attached to, a (C 3 _ 7 )heterocycloalkyl optionally substituted with one or more fluorine, hydroxyl, (Ci_3)alkyl, (Ci_3)alkoxy or oxo;
  • R4 is H or (Ci_ 3 )alkyl;
  • R 5 is H, halogen, cyano, (Ci_4)alkyl, (Ci_ 3 )alkoxy, (C 3 _ 6 )cycloalkyl; all alkyl groups of R5 are optionally substituted with one or more halogen; or R 5 is (C 6 -io)aryl or (C 2- 6 )heterocycloalkyl;
  • Re is H or (Ci_ 3 )alkyl; or R 5 and Re together may form a (C 3 _ 7 )cycloalkenyl, or (C 2- 6 )heterocycloalkenyl; each optionally substituted with (Ci_ 3 )alkyl, or one or more halogen;
  • R 7 is H, halogen, CF 3 , (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy;
  • Rg is H, halogen, CF 3 , (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy; or
  • R9 is H, halogen, (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy; Rio is H, halogen, (Ci_ 3 )alkyl or (Ci_ 3 )alkoxy;
  • R 11 is independently selected from a group consisting of (Ci_ 6 )alkyl, (C 2 _ 6 )alkenyl and (C 2 _
  • alkynyl each alkyl, alkenyl or alkynyl optionally substituted with one or more groups selected from hydroxyl, (Ci_4)alkyl, (C 3 _ 7 )cycloalkyl, [(Ci_4)alkyl]amino, di[(Ci_
  • R 11 is (Ci_ 3 )alkyl-C(0)-S-(Ci_ 3 )alkyl; or
  • R 11 is (Ci_ 5 )heteroaryl optionally substituted with one or more groups selected from halogen or cyano.
  • Ri 2 and Ri 3 are independently selected from a group consisting of (C 2 _ 6 )alkenyl or (C 2 _ 6 )alkynyl both optionally substituted with one or more groups selected from hydroxyl, (Ci_ 4 )alkyl, (C 3 _
  • Ri4 is independently selected from a group consisting of halogen, cyano or (C 2 _ 6 )alkenyl or (C 2 _ 6)alkynyl both optionally substituted with one or more groups selected from hydroxyl, (Ci_ 4 )alkyl, (C 3 _ 7 )cycloalkyl, [(Ci_ 4 )alkyl] amino, di[(Ci_ 4 )alkyl]amino, (Ci_3)alkoxy, (C 3 _
  • X, Y, Z can simultaneously be a heteroatom
  • (Ci_ 3 )alkyl means a branched or unbranched alkyl group having 1-3 carbon atoms, being methyl, ethyl, propyl or isopropyl;
  • (Ci_ 4 )alkyl means a branched or unbranched alkyl group having 1-4 carbon atoms, being methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl and tert-butyl, (Ci_ 3 )alkyl groups being preferred;
  • (Ci_6)alkyl means a branched or unbranched alkyl group having 1-6 carbon atoms, for example methyl, ethyl, propyl, isopropyl, butyl, tert-butyl, n-pentyl and n-hexyl.
  • (Ci_ 5 )alkyl groups are preferred, (Ci_ 4 )alkyl being most preferred;
  • (Ci_ 2 )alkoxy means an alkoxy group having 1-2 carbon atoms, the alkyl moiety having the same meaning as previously defined;
  • (Ci_ 3 )alkoxy means an alkoxy group having 1-3 carbon atoms, the alkyl moiety having the same meaning as previously defined, with (Ci_ 2 )alkoxy groups preferred;
  • (C 2 _ 3 )alkenyl means an alkenyl group having 2-3 carbon atoms, such as ethenyl or 2- propenyl;
  • (C 2 _ 4 )alkenyl means a branched or unbranched alkenyl group having 2-4 carbon atoms, such as ethenyl, 2-propenyl, isobutenyl or 2-butenyl;
  • (C 2 _ 6 )alkenyl means a branched or unbranched alkenyl group having 2-6 carbon atoms, such as ethenyl, 2-butenyl, and n-pentenyl, with (C 2 _ 4 )alkenyl groups preferred, and (C 2 _ 3 )alkenyl groups even more preferred;
  • (C 2 _ 4 )alkynyl means a branched or unbranched alkynyl group having 2-4 carbon atoms, such as ethynyl, 2-propynyl or 2-butynyl;
  • (C 2 _3)alkynyl means an alkynyl group having 2-3 carbon atoms, such as ethynyl or 2-propynyl;
  • (C 2 _ 6 )alkynyl means a branched or unbranched alkynyl group having 2 _ 6 carbon atoms, such as ethynyl, propynyl, n-butynyl, n-pentynyl, isopentynyl, isohexynyl or n-hexynyl, wtih (C 2 _ 4)alkynyl groups preferred, and (C 2 _ 3 )alkynyl groups more preferred;
  • (C 3 _6)cycloalkyl means a cycloalkyl group having 3-6 carbon atoms, being cyclopropyl
  • (C 3 _ 7 )cycloalkyl means a cycloalkyl group having 3-7 carbon atoms, being cyclopropyl
  • (C 2 _6)heterocycloalkyl means a heterocycloalkyl group having 2-6 carbon atoms, preferably 3-5 carbon atoms, and one or two heteroatoms selected from N, O and/or S, which may be attached via a heteroatom if feasible, or a carbon atom; preferred heteroatoms are N or O; preferred groups are piperidine, morpholine, pyrrolidine and piperazine; a most preferred (C 2 _ 6)heterocycloalkyl is pyrrolidine; and the heterocycloalkyl group may be attached via a heteroatom if feasible;
  • (C 3 _ 7 )heterocycloalkyl means a heterocycloalkyl group having 3-7 carbon atoms, preferably 3-5 carbon atoms, and one or two heteroatoms selected from N, O and/or S; preferred
  • heteroatoms are N or O; preferred (C 3 _ 7 ) heterocycloalkyl groups are azetidinyl, pyrrolidinyl, piperidinyl, homopiperidinyl or morpholinyl; more preferred (C 3 _ 7 )heterocycloalkyl groups are piperidine, morpholine and pyrrolidine; even more preferred are piperidine and pyrrolidine; and the heterocycloalkyl group may be attached via a heteroatom if feasible;
  • (C 3 _ 7 )cycloalkoxy means a cycloalkyl group having 3-7 carbon atoms, with the same meaning as previously defined, attached via a ring carbon atom to an exocyclic oxygen atom;
  • (C 6 -io)aryl means an aromatic hydrocarbon group having 6-10 carbon atoms, such as phenyl, naphthyl, tetrahydronaphthyl or indenyl; the preferred (C 6 -io)aryl group is phenyl;
  • (Ci_5)heteroaryl means a substituted or unsubstituted aromatic group having 1-5 carbon atoms and 1-4 heteroatoms selected from N, O and/or S, wherein the (Ci_5)heteroaryl may optionally be substituted.; preferred (Ci_ 5 )heteroaryl groups are tetrazolyl, imidazolyl, thiadiazolyl, pyridyl, pyrimidyl, triazinyl, thienyl or furyl, and the more preferred (Ci_
  • heteroaryl is pyrimidyl
  • 4)alkyl] amino group is methylamino; di[(Ci_ 4 )alkyl]amino means an amino group, disubstituted with alkyl group(s), each containing 1- 4 carbon atoms and having the same meaning as previously defined; the preferred di[(Ci_ 4)alkyl] amino group is dimethylamino; halogen means means fluorine, chlorine, bromine or iodine;
  • (Ci_3)alkyl-C(0)-S-(Ci_3)alkyl means an alkyl-carbonyl-thio-alkyl group, each of the alkyl
  • (C 3 _7)cycloalkenyl means a cycloalkenyl group having 3-7 carbon atoms, preferably 5-7 carbon atoms; preferred (C 3 _7)cycloalkenyl groups are cyclopentenyl or cyclohexenyl; and cyclohexenyl groups are most preferred;
  • (C2_6)heterocycloalkenyl means a heterocycloalkenyl group having 2-6 carbon atoms, preferably 3-5 carbon atoms; and 1 heteroatom selected from N, O and/or S; the preferred (C 2-
  • heterocycloalkenyl groups are oxycyclohexenyl and azacyclohexenyl groups.
  • the attachment point is at the last group.
  • substituents are optionally substituted, this also includes the alkyl moiety of an alkoxy group.
  • a circle in a ring of Formula (III) indicates that the ring is aromatic.
  • the nitrogen if present in X or Y, may carry a hydrogen.
  • the invention relates to a compound according to Formula (XIX) wherein Bi is C(Ry); B 2 is C(R 8 ); B 3 is C(R 9 ) and B 4 is C(Rio).
  • the BMX inhibitors include, but are not limited to, those compounds described in International Patent Application Publication No. WO 2013/010869, the disclosures of each of which are specifically incorporated by reference herein.
  • the BMX inhibitor is a compound of Formula (IV):
  • L a is CH 2 , O, NH or S;
  • Ar is a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl
  • Y is an optionally substituted group selected from the group consisting of alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl;
  • R 7 and R 8 are each independently H; or R 7 and R 8 taken together form a bond;
  • R 6 is H
  • R is H or (Ci- 6 )alkyl.
  • the BMX inhibitor is ibrutinib or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof.
  • the BMX inhibitor is (R)-l- (3-(4-amino-3-(4-phenoxyphenyl)- lH-pyrazolo[3,4-d]pyrimidin- 1 -yl)piperidin- 1 -yl)prop-2-en- 1-one.
  • the BMX inhibitor is l-[(3 ?)-3-[4-amino-3-(4-phenoxyphenyl)-lH- pyrazolo[3,4- ⁇ i]pyrimidin-l-yl]piperidin-l-yl]prop-2-en-l-one.
  • the BMX inhibitor is (S)- 1 -(3 -(4-amino-3 -(4-phenoxyphenyl)- 1 H-pyrazolo [3 ,4- ]pyrimidin- 1 -yl)piperidin- l-yl)prop-2-en-l-one.
  • the BMX inhibitor has the structure of Formula (V), or an enantiomer thereof, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof.
  • the BMX inhibitor is a compound of Formula (VI):
  • L a is CH 2 , O, NH or S
  • Ar is a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl
  • Y is an optionally substituted group selected from the group consisting of alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl;
  • R 7 and R 8 are each H; or R 7 and R 8 taken together form a bond;
  • R 6 is H
  • R is H or (Ci- 6 )alkyl.
  • the BMX inhibitor is a compound of Formula (VII):
  • L a is CH 2 , O, NH or S
  • Ar is a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl
  • Y is an optionally substituted group selected from the group consisting of alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl;
  • R 7 and R 8 are each H; or R 7 and R 8 taken together form a bond;
  • R 6 is H
  • the BMX inhibitor is a compound of Formula (VIII):
  • L a is CH 2 , O, NH or S
  • Ar is a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl
  • Y is an optionally substituted group selected from the group consisting of alkyl, heteroalkyl, cycloalkyl, heterocycloalkyl, aryl and heteroaryl;
  • R 7 and R 8 are each H; or R 7 and R 8 taken together form a bond;
  • R 6 is H; and R is H or (Ci- 6 )alkyl.
  • the BMX inhibitor is a compound disclosed in U.S. Patent No. 7,459,554, the disclosure of which is specifically incorporated herein by reference.
  • the BMX inhibitor is a compound of Formula (IX):
  • Q 1 is aryl 1 , heteroaryl 1 , cycloalkyl, heterocyclyl, cycloalkenyl, or heterocycloalkenyl, any of which is optionally substituted by one to five independent G 1 substituents;
  • R 1 is alkyl, cycloalkyl, bicycloalkyl, aryl, heteroaryl, aralkyl, heteroaralkyl, heterocyclyl, or heterobicycloalkyl, any of which is optionally substituted by one or more independent G 11 substituents;
  • G 1 and G 41 are each independently halo, oxo, -CF 3 , -OCF 3 , -OR 2 , -NR 2 R 3 (R 3a ) j i, -C(0)R
  • R 2 , R 2a , R 3 , R 3a , R 222 , R 222 a, R 333 , R 333a , R 21 , R 2al , R 31 , R 3al , R 2221 , R 222al , R 3331 , and R 333al are each independently equal to (Co-io)alkyl, (C 2 -io)alkenyl, (C 2 -io)alkynyl, (Ci-io)alkoxy(Ci- io)alkyl, (C 1 - 10 )alkoxy(C 2 - 10 )alkenyl, (C 1 - 10 )alkoxy(C 2 - 10 )alkynyl, (C 1 - 10 )alkylthio(C 1 - io)alkyl, (Ci-i 0 )alkylthio(C 2 -i 0 )alkenyl, (Ci-i 0
  • Y 1 and Y 1 are each independently represented by one of the following structural formulas:
  • R taken together with the phosphinamide or phosphonamide, is a 5-, 6-, or 7-membered aryl, heteroaryl or heterocyclyl ring system;
  • R 5 , R 6 , and G 111 are each independently a (Co-io)alkyl, (C 2 -io)alkenyl, (C 2 -io)alkynyl, (Ci- io)alkoxy(Ci-io)alkyl, (Ci-i 0 )alkoxy(C 2 -i 0 )alkenyl, (Ci-i 0 )alkoxy(C 2 -i 0 )alkynyl, (C io)alkylthio(Ci-io)alkyl, (Ci-io)alkylthio(C 2 -io)alkenyl, (Ci-io)alkylthio(C 2 -io)alkynyl, cyclo(C 3 -8)alkyl, cyclo(C 3 -s)alkenyl, cyclo(C 3 -8)alkyl(Ci-io)alkyl
  • R 7 and R 8 are each independently H, acyl, alkyl, alkenyl, aryl, heteroaryl, heterocyclyl or
  • R 4 is H, alkyl, alkenyl, alkynyl, aryl, heteroaryl, cycloalkyl, heterocyclyl, cycloalkenyl, or
  • heterocycloalkenyl any of which is optionally substituted by one or more G 41 substituents;
  • R 69 is equal to halo, -OR 78 , -SH, -NR 78 R 88 , -C0 2 R 78 , -CONR 78 R 88 , -N0 2 , -CN, -S(0) j8 R 78 ,
  • heterocyclyl-(C 2 -io)alkynyl any of which is optionally substituted with one or more independent halo, cyano, nitro, -OR 778 , -S0 2 NR 778 R 888 , or -NR 778 R 888 substituents; or aryl- (Co-io)alkyl, aryl-(C 2 -io)alkenyl, or aryl-(C 2 -io)alkynyl, any of which is optionally substituted with one or more independent halo, cyano, nitro, -OR 778 , (Ci-io)alkyl, (C 2 -i 0 )alkenyl, (C 2 - io)alkynyl, halo(Ci-io)alkyl, halo(C 2 -io)alkenyl, halo(C 2 -io)alkynyl, -COOH, (Ci
  • R 77 , R 78 , R 87 , R 88 , R 778 , and R 888 are each independently (C 0 -io)alkyl, (C 2 -i 0 )alkenyl, (C 2 - io)alkynyl, (Ci-i 0 )alkoxy(Ci-i 0 )alkyl, (Ci-i 0 )alkoxyC 2 -i 0 )alkenyl, (Ci-i 0 )alkoxy(C 2 - io)alkynyl, (Ci-io)alkylthio(Ci-io)alkyl, (Ci-io)alkylthio(C 2 -io)alkenyl, (Ci-io)alkylthio(C 2 -io)alkynyl, cyclo(C 3 -s)alkyl, cyclo(C 3 -s)alkyl
  • the BMX inhibitor is a compound selected from the structures dislosed in U.S. Patent Nos. 8,450,335 and 8,609,679, and U.S. Patent Application Publication Nos. 2010/0029610 Al , 2012/0077832 Al , 2013/0065879 Al , 2013/0072469 Al , and
  • the BMX inhibitor is a compound of Formula (X) or Formula (XI):
  • Ring A is an optionally substituted group selected from phenyl, a 3-7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 7-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ring B is an optionally substituted group selected from phenyl, a 3-7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 7-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 1 is a warhead group
  • R y is hydrogen, halogen,— CN,— CF 3 , Ci_ 4 aliphatic, Ci_ 4 haloaliphatic,— OR,— C(0)R, or— C(0)N(R) 2 ; each R group is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, phenyl, a 4-7 membered heterocylic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • W 1 and W 2 are each independently a covalent bond or a bivalent Ci_ 3 alkylene chain wherein one methylene unit of W 1 or W 2 is optionally replaced by— NR 2 — ,— N(R 2 )C(0)— ,—
  • R 2 is hydrogen, optionally substituted Ci_ 6 aliphatic, or— C(0)R, or:
  • R 2 and a substituent on Ring A are taken together with their intervening atoms to form a 4-6 membered saturated, partially unsaturated, or aromatic fused ring, or:
  • R 2 and R y are taken together with their intervening atoms to form a 4-7 membered partially
  • R x and R v are independently selected from— R, halogen,—OR,— 0(CH 2 ) q OR,— CN,— N0 2 , — S0 2 R,— S0 2 N(R) 2 ,— SOR,— C(0)R,— C0 2 R,— C(0)N(R) 2 ,— NRC(0)R,—
  • NRC(0)NR 2 ,— NRS0 2 R, or— N(R) 2 , wherein q is 1-4; or:
  • R x and R 1 when concurrently present on Ring B are taken together with their intervening atoms to form a 5-7 membered saturated, partially unsaturated, or aryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is substituted with a warhead group and 0-3 groups independently selected from oxo, halogen,— CN, or Ci_ 6 aliphatic; or
  • R v and R 1 when concurrently present on Ring A are taken together with their intervening atoms to form a 5-7 membered saturated, partially unsaturated, or aryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is substituted with a warhead group and 0-3 groups independently selected from oxo, halogen,— CN, or Ci_ 6 aliphatic.
  • the BMX inhibitor is a compound of Formula (X) or Formula (XI), wherein:
  • Ring A is an optionally substituted group selected from phenyl, a 3-7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 7-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ring B is an optionally substituted group selected from phenyl, a 3-7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 7-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 1 is -L-Y, wherein:
  • Y is hydrogen, Ci_ 6 aliphatic optionally substituted with oxo, halogen, or CN, or a 3-10
  • Q is a covalent bond or a bivalent Ci_ 6 saturated or unsaturated, straight or branched,
  • hydrocarbon chain wherein one or two methylene units of Q are optionally and
  • Z is hydrogen or Ci_ 6 aliphatic optionally substituted with oxo, halogen, or CN;
  • R y is hydrogen, halogen,— CN,— CF 3 , Ci_ 4 aliphatic, Ci_ 4 haloaliphatic,— OR,— C(0)R, or— C(0)N(R) 2 ; each R group is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, phenyl, a 4-7 membered heterocylic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • W 1 and W 2 are each independently a covalent bond or a bivalent Ci_ 3 alkylene chain wherein one methylene unit of W 1 or W 2 is optionally replaced by— NR 2 — ,— N(R 2 )C(0)— ,—
  • R 2 is hydrogen, optionally substituted Ci_ 6 aliphatic, or— C(0)R, or:
  • R 2 and a substituent on Ring A are taken together with their intervening atoms to form a 4-6 membered partially unsaturated or aromatic fused ring;
  • R 2 and R y are taken together with their intervening atoms to form a 4-6 membered saturated, partially unsaturated, or aromatic fused ring; m and p are independently 0-4; and
  • R x and R v are independently selected from— R, halogen,—OR,— 0(CH 2 ) q OR,— CN,— N0 2 , — S0 2 R,— S0 2 N(R) 2 ,— SOR,— C(0)R,— C0 2 R,— C(0)N(R) 2 ,— NRC(0)R,—
  • NRC(0)NR 2 — NRS0 2 R, or— N(R) 2 , or:
  • R x and R 1 when concurrently present on Ring B are taken together with their intervening atoms to form a 5-7 membered saturated, partially unsaturated, or aryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is substituted with a warhead group and 0-3 groups independently selected from oxo, halogen,— CN, or Ci_ 6 aliphatic; or
  • R v and R 1 when concurrently present on Ring A are taken together with their intervening atoms to form a 5-7 membered saturated, partially unsaturated, or aryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is substituted with a warhead group and 0-3 groups independently selected from oxo, halogen,— CN, or Ci_ 6 aliphatic.
  • Ring A is an optionally substituted group selected from phenyl, a 3- 7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 7-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • phenyl a 3- 7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring
  • Ring A is an optionally substituted phenyl group. In some embodiments, Ring A is an optionally substituted naphthyl ring or a bicyclic 8-10 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In certain other embodiments, Ring A is an optionally substituted 3-7 membered carbocyclic ring. In other embodiments, Ring A is an optionally substituted 4-7 membered heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • Ring A is substituted as defined herein.
  • Ring A is substituted with one, two, or three groups independently selected from halogen, R°, or— (CH 2 )o- 4 0R°, or— 0(CH 2 )o- 4 R°, wherein each R° is as defined herein.
  • substituents on Ring A include Br, I, CI, methyl,— CF 3 ,— C ⁇ CH,— OCH 2 phenyl,— OCH 2 (fluorophenyl), or— OCH 2 pyridyl.
  • the BMX inhibitor is a compound of Formula (XII), also known as CC-292 (Celgene):
  • the BMX inhibitor is a compound selected from the structures disclosed in U.S. Patent Application Publication No. 2010/0029610 Al or No. 2012/0077832 Al , the disclosures of which are incorporated by reference herein.
  • the BMX inhibitor is N-(3-((5-fluoro-2-((4-(2- methoxyethoxy)phenyl)amino)pyrimidin-4-yl)amino)phenyl)acrylamide or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, or a hydrochloride salt thereof.
  • the preparation of this compound is described in U.S. Patent Application Publication No.
  • the BMX inhibitor is (N-(3-(5-fluoro-2-(4-(2- methoxyethoxy)phenylamino)pyrimidin-4-ylamino)phenyl)acrylamide), or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, or a besylate salt thereof.
  • the preparation of this compound is described in U.S. Patent Application Publication No.
  • the BMX inhibitor is a compound of Formula (XIII):
  • L represents (1) -0-, (2) -S-, (3) -SO-, (4) -S0 2 - (5) -NH-, (6) -C(O)-, (7) -CH 2 0- (8) -O- CH 2 - (9) -CH 2 - or (10) -CH(OH)-;
  • R 1 represents (1) a halogen atom, (2) a Ci_ 4 alkyl group, (3) a Ci_ 4 alkoxy group, (4) a Ci_ 4
  • ringl represents a 4- to 7-membered cyclic group, which may be substituted by from one to five substituents each independently selected from the group consisting of (1) halogen atoms, (2) Ci_4 alkyl groups, (3) Ci_ 4 alkoxy groups, (4) nitrile, (5) Ci_ 4 haloalkyl groups, and (6) Ci_ 4 haloalkoxy groups, wherein when two or more substituents are present on ringl, these substituents may form a 4- to 7-membered cyclic group together with the atoms in ringl to which these substituents are bound; ring2 represents a 4- to 7-membered saturated heterocycle, which may be substituted by from one to three -K-R 2 ; K represents (1) a bond, (2) a Ci_ 4 alkylene, (3) -C(O)-, (4) -C(0)-CH 2 - , (5)
  • R 2 represents (1) a Ci_ 4 alkyl, (2) a C 2 _ 4 alkenyl, or (3) a C 2 _ 4 alkynyl group, each of which may be substituted by from one to five substituents each independently selected from the group consisting of (1) NR 3 R 4 , (2) halogen atoms, (3) CONR 5 R 6 , (4) C0 2 R 7 , and (5) OR 8 ;
  • R 3 and R 4 each independently represent (1) a hydrogen atom, or (2) a Ci_ 4 alkyl group which may be substituted by OR 9 or CONR 10 R n ; R 3 and R 4 may, together with the nitrogen atom to which they are bound, form a 4- to 7-membered nitrogenous saturated heterocycle, which may be substituted by an oxo group or a hydroxyl group;
  • R 5 and R 6 each independently represent (1) a hydrogen atom, (2) a Ci_ 4 alkyl group, or (3) a phenyl group;
  • R 7 represents (1) a hydrogen atom or (2) a Ci_ 4 alkyl group
  • R 8 represents (1) a hydrogen atom, (2) a Ci_ 4 alkyl group, (3) a phenyl group, or (4) a
  • R 9 represents (1) a hydrogen atom or (2) a Ci_ 4 alkyl group
  • R 10 and R 11 each independently represent (1) a hydrogen atom or (2) a Ci_ 4 alkyl group; n represents an integer from 0 to 4; m represents an integer from 0 to 2; and when n is two or more, the R s may be the same as each other or may differ from one another).
  • the BMX inhibitor is a compound of Formula (XIV): Formula (XIV)
  • R 1 represents (1) a halogen atom, (2) a Ci_ 4 alkyl group, (3) a Ci_ 4 alkoxy group, (4) a Ci_ 4
  • ringl represents a benzene, cyclohexane, or pyridine ring, each of which may be substituted by from one to five substituents each independently selected from the group consisting of (1) halogen atoms, (2) Ci_ 4 alkyl groups, (3) Ci_ 4 alkoxy groups, (4) nitrile, (5) CF 3 ;
  • ring2 represents a 4- to 7-membered nitrogenous saturated heterocycle, which may be substituted by from one to three -K-R 2 ; wherein K represents (1) a bond, (2) a Ci_ 4 alkylene, (3) -C(O)-, (4) -C(0)-CH 2 - (5) -CH 2 -C(0)-, (6) -C(0)0- or (7) -S0 2 - (wherein the bond on the left is bound to the ring2);
  • R 2 represents (1) a Ci_ 4 alkyl, (2) a C 2 _ 4 alkenyl, or (3) a C 2 _ 4 alkynyl group, each of which may be substituted by from one to five substituents each independently selected from the group consisting of (1) NR 3 R 4 , (2) halogen atoms, (3) CONR 5 R 6 , (4) C0 2 R 7 , and (5) OR 8 ;
  • R 3 and R 4 each independently represent (1) a hydrogen atom, or (2) a Ci_ 4 alkyl group which may be substituted by OR 9 or CONR 10 R n ; R 3 and R 4 may, together with the nitrogen atom to which they are bound, form a 4- to 7-membered nitrogenous saturated heterocycle, which may be substituted by an oxo group or a hydroxyl group;
  • R 5 and R 6 each independently represent (1) a hydrogen atom, (2) a Ci_ 4 alkyl group, or (3) a
  • R 7 represents (1) a hydrogen atom or (2) a Ci_ 4 alkyl group
  • R 8 represents (1) a hydrogen atom, (2) a Ci_ 4 alkyl group, (3) a phenyl group, or (4) a benzotriazolyl group
  • R 9 represents (1) a hydrogen atom or (2) a Ci_ 4 alkyl group
  • R 10 and R 11 each independently represent (1) a hydrogen atom or (2) a Ci_ 4 alkyl group; n represents an integer from 0 to 4; m represents an integer from 0 to 2; and when n is two or more, the R s may be the same as each other or may differ from one another).
  • the BMX inhibitor is a compound of Formula (XV):
  • the BMX inhibitor is 6- amino-9-( 1 -(but-2-ynoyl)pyrrolidin-3 -yl)-7-(4-phenoxyphenyl)-7,9-dihydro-8H-purin-8-one or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, or a
  • the BMX inhibitor is 6-amino-9-[(35)-l-(2- butynoyl)-3-pyrrolidinyl]-7-(4-phenoxyphenyl)-7,9-dihydro-8H-purin-8-one or a
  • the BMX inhibitor is 6-amino-9-[(3R)-l-(2-but noyl)-3- pyrrolidinyl]-7-(4- phenoxyphenyl)-7,9-dihydro-8H-purin-8-one or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, or a hydrochloride salt thereof.
  • Step 1 A solution of dibenzylamine (10.2 g) in dichloromethane (30 mL) is dripped into a solution of 4,6-dichloro-5-nitropyrimidine (10 g) in dichloromethane (70 mL) on an ice bath. Then triethylamine (14.4 mL) is added, and the mixture is stirred for 1 hour. Water is added to the reaction mixture, the organic layer is washed with a saturated aqueous sodium chloride solution and dried over anhydrous sodium sulfate, and the solvent is concentrated under reduced pressure to obtain N,N-dibenzyl-6-chloro-5-nitropyrimidine-4-amine (19.2 g).
  • Step 2 The compound prepared in Step 1 (19 g) and tert-butyl (3R)-3- aminopyrrolidine-l-carboxylate (10.5 g) are dissolved in dioxane (58 mL). Triethylamine (8.1 mL) is added, and the mixture is stirred for 5 hours at 50° C. The reaction mixture is returned to room temperature, the solvent is distilled off, water is added, and extraction is performed with ethyl acetate. The organic layer is washed with saturated aqueous sodium chloride solution, then dried over anhydrous sodium sulfate, and the solvent is distilled off.
  • Step 3 An ethyl acetate (360 mL) solution of the compound prepared in Step 2 (17.5 g) is dripped into a mixture of zinc (23.3 g) and a 3.0 M aqueous ammonium chloride solution (11.4 g) on an ice bath, and the temperature is immediately raised to room temperature. After stirring for 2 hours, the reaction mixture is filtered through CELITE and the solvent is distilled off. The residue is purified by silica gel column chromatography to obtain tert-butyl (3i?)-3- ⁇ [5- amino-6-(dibenzylamino)pyrimidin-4-yl] amino ⁇ pyrrolidine- 1 -carboxylate ( 12.4 g).
  • Step 4 The compound prepared in Step 3 (8.4 g) and ⁇ , ⁇ -carbonyl diimidazole (5.9 g) are dissolved in tetrahydrofuran (120 mL) and the solution is stirred for 15 hours at 60° C. The solvent is distilled off from the reaction mixture, water is added, and extraction with ethyl acetate is performed. The organic layer is washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulfate, and the solvent is distilled off.
  • Step 5 The compound prepared in Step 4 (7.8 g) is dissolved in methanol (240 mL) and ethyl acetate (50 mL), 20% Pearlman's catalyst (Pd(OH) 2 /C) (8.0 g, 100 wt %) is added, hydrogen gas replacement is carried out, and stirring is performed for 7.5 hours at 60° C.
  • the reaction mixture is filtered through CELITE and the solvent is distilled off to obtain tert-butyl (3i?)-3-(6-amino-8-oxo-7,8-dihydro-9H-purin-9-yl)pyrrolidine- 1 -carboxylate (5.0 g).
  • Step 6 At room temperature p-phenoxy phenyl boronic acid (2.1 g), copper(II) acetate (1.48 g), molecular sieve 4A (2.5 g), and pyridine (0.82 mL) are added to a dichloromethane suspension (200 mL) of the compound prepared in Step 5 (2.5 g), followed by stirring for 21 hours.
  • reaction mixture is filtered through CELITE and the residue is purified by silica gel column chromatography to obtain tert-butyl (3i?)-3-[6-amino-8-oxo-7-(4-phenoxyphenyl)-7,8- dihydro-9H-purin-9-yl]pyrrolidine-l-carboxylate (1.3 g).
  • Step 7 At room temperature 4 N HCl/dioxane (13 mL) is added to a methanol (13 mL) suspension of the compound prepared in Step 6 (1.3 g 2.76 mmol, 1.0 equivalent), and the mixture is stirred for 1 hour. The solvent is then distilled off to obtain (3R)-6-amino-9- pyrrolidin-3-yl-7-(4-phenoxyphenyl)-7,9-dihydro-8H-purin-8-one dihydrochloride (1.5 g).
  • Step 8 After 2-butylnoic acid (34 mg), l-ethyl-3-(3-dimethylaminopropyl)
  • the hydrochloride salt of the compound of Formula (XVI) can be prepared as follows: 6-amino-9-[(3i?)-l-(2-butynoyl)-3-pyrrolidinyl]-7-(4-phenoxyphenyl)-7,9-dihydro-8H-purin-8- one (3.0 g) (which may be prepared as described above) is placed in a 300 mL 3-neck pear- shaped flask, ethyl acetate (30 mL) and 1-propanol (4.5 mL) are added, and the external temperature is set at 70° C (internal temperature 61° C).
  • the BMX inhibitor is a compound selected from the structures disclosed in U.S. Patent Application Publication No. US 2014/0330015 Al, the disclosure of which is incorporated by reference herein.
  • the BMX inhibitor is a compound of Formula (XVII):
  • X-Y-Z is N-C-C and R 2 is present, or C-N-N and R 2 is absent;
  • R 1 is a 3-8 membered, N-containing ring, wherein the N is unsubstituted or substituted with R 4 ;
  • R 2 is H or lower alkyl, particularly methyl, ethyl, propyl or butyl; or
  • R 1 and R 2 together with the atoms to which they are attached, form a 4-8 membered ring,
  • a 5-6 membered ring selected from cycloalkyl, saturated or unsaturated heterocycle, aryl, and heteroaryl rings unsubstituted or substituted with at least one substituent L-R 4 ;
  • R 3 is in each instance, independently halogen, alkyl, S-alkyl, CN, or OR ; n is 1, 2, 3, or 4, preferably 1 or 2;
  • L is a bond, NH, heteroalkyl, or heterocyclyl
  • R 4 is COR, C0 2 R', or S0 2 R, wherein R' is substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl;
  • R 5 is H or unsubstituted or substituted heteroalkyl, alkyl, cycloalkyl, saturated or unsaturated heterocyclyl, aryl, or heteroaryl.
  • the BMX inhibitor is one of the following particular embodiments of Formula (XVII): X--Y--Z is C--N--N and R 2 is absent; and R 1 is 3-8 membered, N-containing ring, N-substituted with R 4 ;
  • X--Y--Z is N--C--C and R 2 is present, R 1 is 3-8 membered, N-containing ring, N-substituted with R 4 ; and R 2 is H or lower alkyl;
  • X--Y--Z is N--C--C and R 2 is present; and R 1 and R 2 together with the atoms to which they are attached, form a 4-8 membered ring selected from cycloalkyl, saturated or unsaturated heterocycle, aryl, and heteroaryl rings unsubstituted or substituted with at least one substituent L-R 4 , wherein preferred rings of R 1 and R 2 are 5-6-membered, particularly dihydropyrrole, tetrahydropyridine, tetrahydroazepine, phenyl, or pyridine;
  • X--Y--Z is N--C--C and R 2 is present; and R 1 and R 2 together with the atoms to which they are attached, form a 5-6 membered ring, preferably (a) phenyl substituted with a single -L-R 4 , or (b) dihydropyrrole or tetrahydropyridine, N-substituted with a single -L-R 4 wherein L is bond;
  • R 1 is piperidine or azaspiro[3.3]heptane, preferably N-substituted with R 4 ;
  • R 4 is COR or S0 2 R, particularly wherein R' is substituted or unsubstituted alkenyl, particularly substituted or unsubstituted ethenyl; or
  • R 5 is unsubstituted or substituted alkyl or aryl, particularly substituted or unsubstituted phenyl or methyl, such as cyclopropyl-substituted methyl with or tetrabutyl-substituted phenyl.
  • the BMX inhibitor is one of the following particular
  • R 1 is piperidine or azaspiro[3.3]heptane, N-substituted with R 4 , wherein R 4 is H, COR or S0 2 R, and R is substituted or unsubstituted alkenyl, particularly substituted or unsubstituted ethenyl;
  • R 3 is -OR 5 , R 5 is phenyl, and n is 1;
  • R 1 and R 2 together with the atoms to which they are attached, form a 5-6 membered ring,
  • tetrahydropyridine N-substituted with a single -L-R 4 wherein L is bond; R 3 is -OR 5 ; n is 1; R 4 is COR, and R is ethenyl; and R 5 is phenyl; and X--Y-Z is C--N--N and R 2 is absent; R 1 is piperidine, N-substituted with R 4 ; R 3 is -OR 5 ; n is 1; R 4 is COR, and R is unsubstituted or substituted alkenyl, particularly ethenyl; and R 5 is substituted or unsubstituted aryl, particularly phenyl.
  • the BMX inhibitor is a compound of Formula (XVIII), Formula (XIX), or Formula (XX):
  • BMX inhibitor of Formula (XVIII) can be prepared by the following procedure.
  • Step 4 Preparation of tert-butyl 3 -(tosyloxy)piperidine- 1 -carboxylate :
  • Step 5 Preparation of tert-butyl 3-(5-amino-4-cyano-3-(4-phenoxyphenyl)-lH- pyrazol- 1 -yl)piperidine- 1 -carboxylate :
  • Step 6 Preparation of tert-butyl 3-(5-amino-4-carbamoyl-3-(4-phenoxyphenyl)-lH- pyrazol- 1 -yl)piperidine- 1 -carboxylate :
  • Step 8 Preparation of l-(l-acryloylpiperidin-3-yl)-5-amino-3-(4-phenoxyphenyl)-lH- pyrazole-4-carboxamide:
  • the enantiomers of Formula (XVIII) provided by the procedure above may be prepared from 5-amino-3-(phenoxyphenyl)-lH-pyrazole-4-carbonitrile and (S)-tert-butyl 3- hydroxypiperidine-l-carboxylate using a similar procedure (step 4 to 8) for Formula (XIX), or from (R)-tert-butyl 3-hydroxypiperidine-l-carboxylate using a similar procedure (step 4 to 8) for Formula (XX).
  • a racemic mixture of Formula (XVIII) may be separated by chiral HPLC, the crystallization of chiral salts, or other means described above to yield Formula (XIX) and Formula (XX) of high enantiomeric purity.
  • the BMX inhibitor is a compound selected from the structures disclosed in U.S. Patent Application Publication No. US 2015/0005277A1, the disclosure of which is incorporated by reference herein.
  • the BMX inhibitor is PD168393, as described in Fry, et ah, Proc. Natl. Acad. Sci. USA 1998, 95, 12022-27.
  • the BMX inhibitor is a compound of Formula (XXI):
  • the BMX inhibitor is CI- 1033, as described in Hur, et ah, Bioorg. Med. Chem. Lett. 2008, 18, 5916-5919.
  • the BMX inhibitor is a compound of Formula (XXII):
  • BMX inhibitors suitable for use in the described combinations also include, but are not limited to, those described in International Patent Application Publication Nos. WO 2013/010868, WO 2012/158843, WO 2012/135944, WO 2012/135937, U.S. Patent Application Publication No. 2011/0177011, and U.S. Patent Nos. 8,501,751, 8,476,284, 8,008,309,
  • the invention provides pharmaceutical compositions for treating solid tumor cancers, lymphomas and leukemia.
  • the pharmaceutical compositions are typically formulated to provide a therapeutically effective amount of a BMX inhibitor as the active ingredients, or a pharmaceutically acceptable salt, ester, prodrug, solvate, hydrate or derivative thereof.
  • the pharmaceutical compositions contain a pharmaceutically acceptable salt and/or coordination complex thereof, and one or more pharmaceutically acceptable excipients, carriers, including inert solid diluents and fillers, diluents, including sterile aqueous solution and various organic solvents, permeation enhancers, solubilizers and adjuvants.
  • compositions are administered as a BMX inhibitor.
  • other agent(s) may be mixed into a preparation or both components may be formulated into separate preparations for use in combination separately or at the same time.
  • the concentration of each of the BMX inhibitors provided in the pharmaceutical compositions of the invention is independently less than, for example, 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 19%, 18%, 17%, 16%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.4%, 0.3%, 0.2%, 0.1%, 0.09%, 0.08%, 0.07%, 0.06%, 0.05%, 0.04%, 0.03%, 0.02%, 0.01%, 0.009%, 0.008%, 0.007%, 0.006%, 0.005%, 0.004%, 0.003%, 0.002%, 0.001%, 0.0009%, 0.0008%, 0.0007%, 0.0006%, 0.0005%, 0.0004%, 0.0003%, 0.0002% or 0.0001% w/w, w/v or v/v, relative to the total mass or volume
  • the concentration of each of the BMX inhibitors provided in the pharmaceutical compositions of the invention is independently greater than 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 19.75%, 19.50%, 19.25% 19%, 18.75%, 18.50%, 18.25% 18%, 17.75%, 17.50%, 17.25% 17%, 16.75%, 16.50%, 16.25% 16%, 15.75%, 15.50%, 15.25% 15%, 14.75%, 14.50%, 14.25% 14%, 13.75%, 13.50%, 13.25% 13%, 12.75%, 12.50%, 12.25% 12%, 11.75%, 11.50%, 11.25% 11%, 10.75%, 10.50%, 10.25% 10%, 9.75%, 9.50%, 9.25% 9%, 8.75%, 8.50%, 8.25% 8%, 7.75%, 7.50%, 7.25% 7%, 6.75%, 6.50%, 6.25% 6%, 5.75%, 5.50%, 5.25%
  • the concentration of each of the BMX inhibitors of the invention is independently in the range from approximately 0.0001% to approximately 50%, approximately 0.001% to approximately 40%, approximately 0.01% to approximately 30%, approximately 0.02% to approximately 29%, approximately 0.03% to approximately 28%, approximately 0.04% to approximately 27%, approximately 0.05% to approximately 26%, approximately 0.06% to approximately 25%, approximately 0.07% to approximately 24%, approximately 0.08% to approximately 23%, approximately 0.09% to approximately 22%, approximately 0.1% to approximately 21%, approximately 0.2% to approximately 20%, approximately 0.3% to approximately 19%, approximately 0.4% to approximately 18%, approximately 0.5% to approximately 17%, approximately 0.6% to approximately 16%, approximately 0.7% to approximately 15%, approximately 0.8% to approximately 14%, approximately 0.9% to approximately 12% or approximately 1% to approximately 10% w/w, w/v or v/v, relative to the total mass or volume of the pharmaceutical composition.
  • the concentration of each of the BMX inhibitors of the invention is independently in the range from approximately 0.001% to approximately 10%, approximately 0.01% to approximately 5%, approximately 0.02% to approximately 4.5%, approximately 0.03% to approximately 4%, approximately 0.04% to approximately 3.5%, approximately 0.05% to approximately 3%, approximately 0.06% to approximately 2.5%, approximately 0.07% to approximately 2%, approximately 0.08% to approximately 1.5%, approximately 0.09% to approximately 1%, approximately 0.1% to approximately 0.9% w/w, w/v or v/v, relative to the total mass or volume of the pharmaceutical composition.
  • the amount of each of the BMX inhibitors of the invention is independently equal to or less than 3.0 g, 2.5 g, 2.0 g, 1.5 g, 1.0 g, 0.95 g, 0.9 g, 0.85 g, 0.8 g, 0.75 g, 0.7 g, 0.65 g, 0.6 g, 0.55 g, 0.5 g, 0.45 g, 0.4 g, 0.35 g, 0.3 g, 0.25 g, 0.2 g, 0.15 g, 0.1 g, 0.09 g, 0.08 g, 0.07 g, 0.06 g, 0.05 g, 0.04 g, 0.03 g, 0.02 g, 0.01 g, 0.009 g, 0.008 g, 0.007 g, 0.006 g, 0.005 g, 0.004 g, 0.003 g, 0.002 g, 0.001 g, 0.0009 g, 0.0008 g, 0.000
  • the amount of each of the BMX inhibitors of the invention is independently more than 0.0001 g, 0.0002 g, 0.0003 g, 0.0004 g, 0.0005 g, 0.0006 g, 0.0007 g, 0.0008 g, 0.0009 g, 0.001 g, 0.0015 g, 0.002 g, 0.0025 g, 0.003 g, 0.0035 g, 0.004 g, 0.0045 g, 0.005 g, 0.0055 g, 0.006 g, 0.0065 g, 0.007 g, 0.0075 g, 0.008 g, 0.0085 g, 0.009 g, 0.0095 g, 0.01 g, 0.015 g, 0.02 g, 0.025 g, 0.03 g, 0.035 g, 0.04 g, 0.045 g, 0.05 g, 0.055 g, 0.06 g, 0.065 g,
  • Each of the BMX inhibitors according to the invention is effective over a wide dosage range.
  • dosages independently range from 0.01 to 1000 mg, from 0.5 to 100 mg, from 1 to 50 mg per day, and from 5 to 40 mg per day are examples of dosages that may be used.
  • the exact dosage will depend upon the route of administration, the form in which the compound is administered, the gender and age of the subject to be treated, the body weight of the subject to be treated, and the preference and experience of the attending physician.
  • compositions for Oral Administration are provided.
  • the invention provides a pharmaceutical composition for oral administration containing the BMX inhibitor, and a pharmaceutical excipient suitable for oral administration.
  • the invention provides a solid pharmaceutical composition for oral administration containing: (i) an effective amount of a BMX inhibitor and (ii) a
  • composition further contains (iii) an effective amount of a fourth compound.
  • the pharmaceutical composition may be a liquid
  • compositions of the invention suitable for oral administration can be presented as discrete dosage forms, such as capsules, cachets, or tablets, or liquids or aerosol sprays each containing a predetermined amount of an active ingredient as a powder or in granules, a solution, or a suspension in an aqueous or non-aqueous liquid, an oil-in-water emulsion, or a water-in-oil liquid emulsion.
  • dosage forms can be prepared by any of the methods of pharmacy, but all methods include the step of bringing the active ingredient(s) into association with the carrier, which constitutes one or more necessary ingredients.
  • compositions are prepared by uniformly and intimately admixing the active ingredient(s) with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product into the desired presentation.
  • a tablet can be prepared by compression or molding, optionally with one or more accessory ingredients.
  • Compressed tablets can be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as powder or granules, optionally mixed with an excipient such as, but not limited to, a binder, a lubricant, an inert diluent, and/or a surface active or dispersing agent. Molded tablets can be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
  • the invention further encompasses anhydrous pharmaceutical compositions and dosage forms since water can facilitate the degradation of some compounds.
  • water may be added (e.g., 5%) in the pharmaceutical arts as a means of simulating long-term storage in order to determine characteristics such as shelf-life or the stability of formulations over time.
  • Anhydrous pharmaceutical compositions and dosage forms of the invention can be prepared using anhydrous or low moisture containing ingredients and low moisture or low humidity conditions.
  • Pharmaceutical compositions and dosage forms of the invention which contain lactose can be made anhydrous if substantial contact with moisture and/or humidity during manufacturing, packaging, and/or storage is expected.
  • An anhydrous pharmaceutical composition may be prepared and stored such that its anhydrous nature is maintained.
  • anhydrous compositions may be packaged using materials known to prevent exposure to water such that they can be included in suitable formulary kits.
  • suitable packaging include, but are not limited to, hermetically sealed foils, plastic or the like, unit dose containers, blister packs, and strip packs.
  • Each of the BMX inhibitors as active ingredients can be combined in an intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques.
  • the carrier can take a wide variety of forms depending on the form of preparation desired for administration.
  • any of the usual pharmaceutical media can be employed as carriers, such as, for example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents, and the like in the case of oral liquid preparations (such as suspensions, solutions, and elixirs) or aerosols; or carriers such as starches, sugars, micro-crystalline cellulose, diluents, granulating agents, lubricants, binders, and disintegrating agents can be used in the case of oral solid preparations, in some embodiments without employing the use of lactose.
  • suitable carriers include powders, capsules, and tablets, with the solid oral preparations. If desired, tablets can be coated by standard aqueous or nonaqueous techniques.
  • Binders suitable for use in pharmaceutical compositions and dosage forms include, but are not limited to, corn starch, potato starch, or other starches, gelatin, natural and synthetic gums such as acacia, sodium alginate, alginic acid, other alginates, powdered tragacanth, guar gum, cellulose and its derivatives (e.g., ethyl cellulose, cellulose acetate, carboxymethyl cellulose calcium, sodium carboxymethyl cellulose), polyvinyl pyrrolidone, methyl cellulose, pre- gelatinized starch, hydroxypropyl methyl cellulose, microcrystalline cellulose, and mixtures thereof.
  • natural and synthetic gums such as acacia, sodium alginate, alginic acid, other alginates, powdered tragacanth, guar gum, cellulose and its derivatives (e.g., ethyl cellulose, cellulose acetate, carboxymethyl cellulose calcium, sodium carboxymethyl cellulose), polyvinyl pyrrol
  • suitable fillers for use in the pharmaceutical compositions and dosage forms disclosed herein include, but are not limited to, talc, calcium carbonate (e.g., granules or powder), microcrystalline cellulose, powdered cellulose, dextrates, kaolin, mannitol, silicic acid, sorbitol, starch, pre-gelatinized starch, and mixtures thereof.
  • Disintegrants may be used in the compositions of the invention to provide tablets that disintegrate when exposed to an aqueous environment. Too much of a disintegrant may produce tablets which disintegrate in the bottle. Too little may be insufficient for disintegration to occur, thus altering the rate and extent of release of the active ingredients from the dosage form. Thus, a sufficient amount of disintegrant that is neither too little nor too much to detrimentally alter the release of the active ingredient(s) may be used to form the dosage forms of the compounds disclosed herein. The amount of disintegrant used may vary based upon the type of formulation and mode of administration, and may be readily discernible to those of ordinary skill in the art.
  • Disintegrants that can be used to form pharmaceutical compositions and dosage forms of the invention include, but are not limited to, agar-agar, alginic acid, calcium carbonate, microcrystalline cellulose, croscarmellose sodium, crospovidone, polacrilin potassium, sodium starch glycolate, potato or tapioca starch, other starches, pre-gelatinized starch, other starches, clays, other algins, other celluloses, gums or mixtures thereof.
  • Lubricants which can be used to form pharmaceutical compositions and dosage forms of the invention include, but are not limited to, calcium stearate, magnesium stearate, mineral oil, light mineral oil, glycerin, sorbitol, mannitol, polyethylene glycol, other glycols, stearic acid, sodium lauryl sulfate, talc, hydrogenated vegetable oil (e.g., peanut oil, cottonseed oil, sunflower oil, sesame oil, olive oil, corn oil, and soybean oil), zinc stearate, ethyl oleate, ethylaureate, agar, or mixtures thereof.
  • Additional lubricants include, for example, a syloid silica gel, a coagulated aerosol of synthetic silica, or mixtures thereof.
  • a lubricant can optionally be added, in an amount of less than about 1 weight percent of the pharmaceutical composition.
  • the essential active ingredient therein may be combined with various sweetening or flavoring agents, coloring matter or dyes and, if so desired, emulsifying and/or suspending agents, together with such diluents as water, ethanol, propylene glycol, glycerin and various combinations thereof.
  • the tablets can be uncoated or coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period.
  • a time delay material such as glyceryl monostearate or glyceryl distearate can be employed.
  • Formulations for oral use can also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example, peanut oil, liquid paraffin or olive oil.
  • Surfactants which can be used to form pharmaceutical compositions and dosage forms of the invention include, but are not limited to, hydrophilic surfactants, lipophilic surfactants, and mixtures thereof. That is, a mixture of hydrophilic surfactants may be employed, a mixture of lipophilic surfactants may be employed, or a mixture of at least one hydrophilic surfactant and at least one lipophilic surfactant may be employed.
  • a suitable hydrophilic surfactant may generally have an HLB value of at least 10, while suitable lipophilic surfactants may generally have an HLB value of or less than about 10.
  • An empirical parameter used to characterize the relative hydrophilicity and hydrophobicity of non- ionic amphiphilic compounds is the hydrophilic-lipophilic balance ("HLB" value).
  • HLB hydrophilic-lipophilic balance
  • Surfactants with lower HLB values are more lipophilic or hydrophobic, and have greater solubility in oils, while surfactants with higher HLB values are more hydrophilic, and have greater solubility in aqueous solutions.
  • Hydrophilic surfactants are generally considered to be those compounds having an HLB value greater than about 10, as well as anionic, cationic, or zwitterionic compounds for which the HLB scale is not generally applicable.
  • lipophilic (i.e., hydrophobic) surfactants are compounds having an HLB value equal to or less than about 10.
  • HLB value of a surfactant is merely a rough guide generally used to enable formulation of industrial, pharmaceutical and cosmetic emulsions.
  • Hydrophilic surfactants may be either ionic or non-ionic. Suitable ionic surfactants include, but are not limited to, alkylammonium salts; fusidic acid salts; fatty acid derivatives of amino acids, oligopeptides, and polypeptides; glyceride derivatives of amino acids,
  • oligopeptides, and polypeptides lecithins and hydrogenated lecithins; lysolecithins and hydrogenated lysolecithins; phospholipids and derivatives thereof; lysophospholipids and derivatives thereof; carnitine fatty acid ester salts; salts of alkylsulfates; fatty acid salts; sodium docusate; acylactylates; mono- and di-acetylated tartaric acid esters of mono- and di-glycerides; succinylated mono- and di-glycerides; citric acid esters of mono- and di-glycerides; and mixtures thereof.
  • ionic surfactants include, by way of example:
  • lecithins lysolecithin, phospholipids, lysophospholipids and derivatives thereof; carnitine fatty acid ester salts; salts of alkylsulfates; fatty acid salts; sodium docusate; acylactylates; mono- and di-acetylated tartaric acid esters of mono- and di-glycerides; succinylated mono- and di- glycerides; citric acid esters of mono- and di-glycerides; and mixtures thereof.
  • Ionic surfactants may be the ionized forms of lecithin, lysolecithin,
  • phosphatidylcholine phosphatidylethanolamine
  • phosphatidylglycerol phosphatidic acid
  • phosphatidylserine lysophosphatidylcholine
  • lysophosphatidylethanolamine phosphatidylethanolamine
  • lysophosphatidylglycerol lysophosphatidic acid, lysophosphatidylserine, PEG- phosphatidylethanolamine, PVP-phosphatidylethanolamine, lactylic esters of fatty acids, stearoyl-2-lactylate, stearoyl lactylate, succinylated monoglycerides, mono/diacetylated tartaric acid esters of mono/diglycerides, citric acid esters of mono/diglycerides, cholylsarcosine, caproate, caprylate, caprate, laurate, myristate, palmitate, oleate, ricinoleate, linoleate, linolenate, stearate, lauryl sulfate, teracecyl sulfate, docusate, lauroyl carnitines, palmitoyl carnitines, myristoyl carn
  • Hydrophilic non-ionic surfactants may include, but not limited to, alkylglucosides; alkylmaltosides; alkylthioglucosides; lauryl macrogolglycerides; polyoxyalkylene alkyl ethers such as polyethylene glycol alkyl ethers; polyoxyalkylene alkylphenols such as polyethylene glycol alkyl phenols; polyoxyalkylene alkyl phenol fatty acid esters such as polyethylene glycol fatty acids monoesters and polyethylene glycol fatty acids diesters; polyethylene glycol glycerol fatty acid esters; polyglycerol fatty acid esters; polyoxyalkylene sorbitan fatty acid esters such as polyethylene glycol sorbitan fatty acid esters; hydrophilic transesterification products of a polyol with at least one member of the group consisting of glycerides, vegetable oils, hydrogenated vegetable oils, fatty acids, and sterols; polyoxyethylene sterol
  • hydrophilic-non-ionic surfactants include, without limitation, PEG- 10 laurate, PEG- 12 laurate, PEG-20 laurate, PEG-32 laurate, PEG-32 dilaurate, PEG- 12 oleate, PEG- 15 oleate, PEG-20 oleate, PEG-20 dioleate, PEG-32 oleate, PEG-200 oleate, PEG-400 oleate, PEG- 15 stearate, PEG-32 distearate, PEG-40 stearate, PEG- 100 stearate, PEG-20 dilaurate, PEG-25 glyceryl trioleate, PEG-32 dioleate, PEG-20 glyceryl laurate, PEG-30 glyceryl laurate, PEG-20 glyceryl stearate, PEG-20 glyceryl oleate, PEG-30 glyceryl oleate, PEG-30 glyce
  • Suitable lipophilic surfactants include, by way of example only: fatty alcohols; glycerol fatty acid esters; acetylated glycerol fatty acid esters; lower alcohol fatty acids esters; propylene glycol fatty acid esters; sorbitan fatty acid esters; polyethylene glycol sorbitan fatty acid esters; sterols and sterol derivatives; polyoxyethylated sterols and sterol derivatives; polyethylene glycol alkyl ethers; sugar esters; sugar ethers; lactic acid derivatives of mono- and di-glycerides;
  • hydrophobic transesterification products of a polyol with at least one member of the group consisting of glycerides, vegetable oils, hydrogenated vegetable oils, fatty acids and sterols; oil- soluble vitamins/vitamin derivatives; and mixtures thereof.
  • preferred lipophilic surfactants include glycerol fatty acid esters, propylene glycol fatty acid esters, and mixtures thereof, or are hydrophobic transesterification products of a polyol with at least one member of the group consisting of vegetable oils, hydrogenated vegetable oils, and triglycerides.
  • the composition may include a solubilizer to ensure good
  • solubilization and/or dissolution of the compound of the present invention and to minimize precipitation of the compound of the present invention. This can be especially important for compositions for non-oral use - e.g., compositions for injection.
  • a solubilizer may also be added to increase the solubility of the hydrophilic drug and/or other components, such as surfactants, or to maintain the composition as a stable or homogeneous solution or dispersion.
  • solubilizers include, but are not limited to, the following: alcohols and polyols, such as ethanol, isopropanol, butanol, benzyl alcohol, ethylene glycol, propylene glycol, butanediols and isomers thereof, glycerol, pentaerythritol, sorbitol, mannitol, transcutol, dimethyl isosorbide, polyethylene glycol, polypropylene glycol, polyvinylalcohol,
  • alcohols and polyols such as ethanol, isopropanol, butanol, benzyl alcohol, ethylene glycol, propylene glycol, butanediols and isomers thereof, glycerol, pentaerythritol, sorbitol, mannitol, transcutol, dimethyl isosorbide, polyethylene glycol, polypropylene glycol, polyvinylalcohol,
  • solubilizers may also be used. Examples include, but not limited to, triacetin, triethylcitrate, ethyl oleate, ethyl caprylate, dimethylacetamide, N-methylpyrrolidone, N-hydroxyethylpyrrolidone, polyvinylpyrrolidone, hydroxypropyl methylcellulose,
  • hydroxypropyl cyclodextrins ethanol, polyethylene glycol 200-100, glycofurol, transcutol, propylene glycol, and dimethyl isosorbide.
  • Particularly preferred solubilizers include sorbitol, glycerol, triacetin, ethyl alcohol, PEG-400, glycofurol and propylene glycol.
  • the amount of solubilizer that can be included is not particularly limited.
  • the amount of a given solubilizer may be limited to a bioacceptable amount, which may be readily determined by one of skill in the art.
  • the solubilizer can be in a weight ratio of 10%, 25%, 50%>, 100%, or up to about 200% by weight, based on the combined weight of the drug, and other excipients.
  • very small amounts of solubilizer may also be used, such as 5%, 2%, 1% or even less.
  • the solubilizer may be present in an amount of about 1% to about 100%, more typically about 5% to about 25% by weight.
  • the composition can further include one or more pharmaceutically acceptable additives and excipients.
  • additives and excipients include, without limitation, detackifiers, anti- foaming agents, buffering agents, polymers, antioxidants, preservatives, chelating agents, viscomodulators, tonicifiers, flavorants, colorants, odorants, opacifiers, suspending agents, binders, fillers, plasticizers, lubricants, and mixtures thereof.
  • an acid or a base may be incorporated into the composition to facilitate processing, to enhance stability, or for other reasons.
  • pharmaceutically acceptable bases include amino acids, amino acid esters, ammonium hydroxide, potassium hydroxide, sodium hydroxide, sodium hydrogen carbonate, aluminum hydroxide, calcium carbonate, magnesium hydroxide, magnesium aluminum silicate, synthetic aluminum silicate, synthetic hydrocalcite, magnesium aluminum hydroxide, diisopropylethylamine, ethanolamine, ethylenediamine, triethanolamine, triethylamine, triisopropanolamine, trimethylamine, tris(hydroxymethyl)aminomethane (TRIS) and the like.
  • bases that are salts of a pharmaceutically acceptable acid, such as acetic acid, acrylic acid, adipic acid, alginic acid, alkanesulfonic acid, amino acids, ascorbic acid, benzoic acid, boric acid, butyric acid, carbonic acid, citric acid, fatty acids, formic acid, fumaric acid, gluconic acid, hydroquinosulfonic acid, isoascorbic acid, lactic acid, maleic acid, oxalic acid, para-bromophenylsulfonic acid, propionic acid, p-toluenesulfonic acid, salicylic acid, stearic acid, succinic acid, tannic acid, tartaric acid, thioglycolic acid, toluenesulfonic acid, uric acid, and the like.
  • a pharmaceutically acceptable acid such as acetic acid, acrylic acid, adipic acid, alginic acid, alkanesulfonic acid, amino acids
  • Salts of polyprotic acids such as sodium phosphate, disodium hydrogen phosphate, and sodium dihydrogen phosphate can also be used.
  • the cation can be any convenient and pharmaceutically acceptable cation, such as ammonium, alkali metals and alkaline earth metals.
  • Example may include, but not limited to, sodium, potassium, lithium, magnesium, calcium and ammonium.
  • Suitable acids are pharmaceutically acceptable organic or inorganic acids.
  • suitable inorganic acids include hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, boric acid, phosphoric acid, and the like.
  • suitable organic acids include acetic acid, acrylic acid, adipic acid, alginic acid, alkanesulfonic acids, amino acids, ascorbic acid, benzoic acid, boric acid, butyric acid, carbonic acid, citric acid, fatty acids, formic acid, fumaric acid, gluconic acid, hydroquinosulfonic acid, isoascorbic acid, lactic acid, maleic acid, methanesulfonic acid, oxalic acid, para-bromophenylsulfonic acid, propionic acid, p- toluenesulfonic acid, salicylic acid, stearic acid, succinic acid, tannic acid, tartaric acid, thioglycolic acid, toluenesulfonic acid and uric acid.
  • the invention provides a pharmaceutical composition for injection containing the BMX inhibitors and a pharmaceutical excipient suitable for injection.
  • Components and amounts of agents in the compositions are as described herein.
  • the forms in which the compositions of the present invention may be incorporated for administration by injection include aqueous or oil suspensions, or emulsions, with sesame oil, corn oil, cottonseed oil, or peanut oil, as well as elixirs, mannitol, dextrose, or a sterile aqueous solution, and similar pharmaceutical vehicles.
  • Aqueous solutions in saline are also conventionally used for injection.
  • Ethanol, glycerol, propylene glycol and liquid polyethylene glycol (and suitable mixtures thereof), cyclodextrin derivatives, and vegetable oils may also be employed.
  • the proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, for the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • the prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid and thimerosal.
  • Sterile injectable solutions are prepared by incorporating the BMX inhibitors in the required amounts in the appropriate solvent with various other ingredients as enumerated above, as required, followed by filtered sterilization.
  • dispersions are prepared by
  • a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above.
  • certain desirable methods of preparation are spray-drying, vacuum-drying and freeze-drying (lyophilization) techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • Other lyophilized or spray-dried antibody formulations known to those of skill in the art may also be employed with the present invention. Such formulations include those disclosed in U.S. Patent Nos. 5,908,826, 6,267,958, 7,682,609, 7,592,004, and 8,298,530, and U.S. Patent Application Publication No. 2010/0158925, the teachings of which are specifically incorporated by reference herein.
  • the invention provides a pharmaceutical composition for transdermal delivery containing the BMX inhibitors and a pharmaceutical excipient suitable for transdermal delivery.
  • compositions of the present invention can be formulated into preparations in solid, semi-solid, or liquid forms suitable for local or topical administration, such as gels, water soluble jellies, creams, lotions, suspensions, foams, powders, slurries, ointments, solutions, oils, pastes, suppositories, sprays, emulsions, saline solutions, dimethylsulfoxide (DMSO)-based solutions.
  • DMSO dimethylsulfoxide
  • carriers with higher densities are capable of providing an area with a prolonged exposure to the active ingredients.
  • a solution formulation may provide more immediate exposure of the active ingredient to the chosen area.
  • compositions also may comprise suitable solid or gel phase carriers or excipients, which are compounds that allow increased penetration of, or assist in the delivery of, therapeutic molecules across the stratum corneum permeability barrier of the skin.
  • suitable solid or gel phase carriers or excipients which are compounds that allow increased penetration of, or assist in the delivery of, therapeutic molecules across the stratum corneum permeability barrier of the skin.
  • penetration-enhancing molecules known to those trained in the art of topical formulation.
  • humectants e.g., urea
  • glycols e.g., propylene glycol
  • alcohols e.g., ethanol
  • fatty acids e.g., oleic acid
  • surfactants e.g., isopropyl myristate and sodium lauryl sulfate
  • pyrrolidones e.g., isopropyl myristate and sodium lauryl sulfate
  • pyrrolidones e.glycerol monolaurate, sulfoxides, terpenes (e.g., menthol)
  • amines amides, alkanes, alkanols, water, calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.
  • transdermal delivery devices patches
  • Such transdermal patches may be used to provide continuous or discontinuous infusion of the BMX inhibitors in controlled amounts, either with or without another agent.
  • transdermal patches for the delivery of pharmaceutical agents is well known in the art. See, e.g., U.S. Patent Nos. 5,023,252; 4,992,445 and 5,001,139. Such patches may be constructed for continuous, pulsatile, or on demand delivery of
  • compositions may also be prepared from compositions described herein and one or more pharmaceutically acceptable excipients suitable for sublingual, buccal, rectal, intraosseous, intraocular, intranasal, epidural, or intraspinal administration. Preparations for such pharmaceutical compositions are well-known in the art. See, e.g., Anderson et al,
  • Administration of the BMX inhibitors or pharmaceutical compositions of these compounds can be effected by any method that enables delivery of the compounds to the site of action. These methods include oral routes, intraduodenal routes, parenteral injection (including intravenous, intraarterial, subcutaneous, intramuscular, intravascular, intraperitoneal or infusion), topical (e.g., transdermal application), rectal administration, via local delivery by catheter or stent or through inhalation.
  • parenteral injection including intravenous, intraarterial, subcutaneous, intramuscular, intravascular, intraperitoneal or infusion
  • topical e.g., transdermal application
  • rectal administration via local delivery by catheter or stent or through inhalation.
  • the combination of compounds can also be administered
  • Parenteral administration forms include solutions or suspensions of active compound in sterile aqueous solutions, for example, aqueous propylene glycol or dextrose solutions. Such dosage forms can be suitably buffered, if desired.
  • kits include the BMX inhibitors, either alone or in combination in suitable packaging, and written material that can include instructions for use, discussion of clinical studies and listing of side effects.
  • kits may also include information, such as scientific literature references, package insert materials, clinical trial results, and/or summaries of these and the like, which indicate or establish the activities and/or advantages of the composition, and/or which describe dosing, administration, side effects, drug interactions, or other information useful to the health care provider. Such information may be based on the results of various studies, for example, studies using experimental animals involving in vivo models and studies based on human clinical trials.
  • the kit may further contain another agent.
  • the BMX inhibitors and the agent are provided as separate compositions in separate containers within the kit. In selected embodiments, the BMX inhibitors and the agent are provided as a single composition within a container in the kit. Suitable packaging and additional articles for use (e.g., measuring cup for liquid preparations, foil wrapping to minimize exposure to air, and the like) are known in the art and may be included in the kit. Kits described herein can be provided, marketed and/or promoted to health providers, including physicians, nurses, pharmacists, formulary officials, and the like. Kits may also, in selected embodiments, be marketed directly to the consumer.
  • BMX inhibitors administered will be dependent on the mammal being treated, the severity of the disorder or condition, the rate of administration, the disposition of the compounds and the discretion of the prescribing physician. However, an effective dosage is in the range of about 0.001 to about 100 mg per kg body weight per day, such as about 1 to about 35 mg/kg/day, in single or divided doses. For a 70 kg human, this would amount to about 0.05 to 7 g/day, such as about 0.05 to about 2.5 g/day.
  • dosage levels below the lower limit of the aforesaid range may be more than adequate, while in other cases still larger doses may be employed without causing any harmful side effect - e.g., by dividing such larger doses into several small doses for administration throughout the day.
  • the BMX inhibitor is administered in a single dose.
  • Such administration will be by injection, for example by intravenous injection, in order to introduce the agents quickly.
  • other routes may be used as appropriate.
  • a single dose of the BMX inhibitor may also be used for treatment of an acute condition.
  • the BMX inhibitor is administered in multiple doses. Dosing may be about once, twice, three times, four times, five times, six times, or more than six times per day. Dosing may be about once a month, once every two weeks, once a week, or once every other day. In other embodiments, the BMX inhibitor is administered about once per day to about 6 times per day. In another embodiment the administration of the combination of the BMX inhibitor continues for less than about 7 days. In yet another embodiment the administration continues for more than about 6, 10, 14, 28 days, two months, six months, or one year. In some cases, continuous dosing is achieved and maintained as long as necessary.
  • the BMX inhibitors of the invention may continue as long as necessary.
  • the BMX inhibitor is administered for more than 1, 2, 3, 4, 5, 6, 7, 14, or 28 days.
  • the BMX inhibitor is administered for less than 28, 14, 7, 6, 5, 4, 3, 2, or 1 day.
  • the BMX inhibitor is administered chronically on an ongoing basis - e.g., for the treatment of chronic effects.
  • an effective dosage of each of the BMX inhibitors is in the range of about 1 mg to about 500 mg, about 10 mg to about 300 mg, about 20 mg to about 250 mg, about 25 mg to about 200 mg, about 10 mg to about 200 mg, about 20 mg to about 150 mg, about 30 mg to about 120 mg, about 10 mg to about 90 mg, about 20 mg to about 80 mg, about 30 mg to about 70 mg, about 40 mg to about 60 mg, about 45 mg to about 55 mg, about 48 mg to about 52 mg, about 50 mg to about 150 mg, about 60 mg to about 140 mg, about 70 mg to about 130 mg, about 80 mg to about 120 mg, about 90 mg to about 110 mg, about 95 mg to about 105 mg, about 150 mg to about 250 mg, about 160 mg to about 240 mg, about 170 mg to about 230 mg, about 180 mg to about 220 mg, about 190 mg to about 210 mg, about 195 mg to about 205 mg, or about 198 to about 202 mg.
  • an effective dosage of each of the BMX inhibitors is about 25 mg, about 50 mg, about 75 mg, about 100 mg, about 125 mg, about 150 mg, about 175 mg, about 200 mg, about 225 mg, about 250 mg, about 275 mg, about 300 mg, about 325 mg, about 350 mg, about 375 mg, about 400 mg, about 425 mg, about 450 mg, about 475 mg, or about 500 mg.
  • an effective dosage of each of the BMX inhibitors is 25 mg, 50 mg, 75 mg, 100 mg, 125 mg, 150 mg, 175 mg, 200 mg, 225 mg, 250 mg, 275 mg, 300 mg, 325 mg, 350 mg, 375 mg, 400 mg, 425 mg, 450 mg, 475 mg, or 500 mg.
  • an effective dosage of each of the BMX inhibitors is in the range of about 0.01 mg/kg to about 4.3 mg/kg, about 0.15 mg/kg to about 3.6 mg/kg, about 0.3 mg/kg to about 3.2 mg/kg, about 0.35 mg/kg to about 2.85 mg/kg, about 0.15 mg/kg to about 2.85 mg/kg, about 0.3 mg to about 2.15 mg/kg, about 0.45 mg/kg to about 1.7 mg/kg, about 0.15 mg/kg to about 1.3 mg/kg, about 0.3 mg/kg to about 1.15 mg/kg, about 0.45 mg/kg to about 1 mg/kg, about 0.55 mg/kg to about 0.85 mg/kg, about 0.65 mg/kg to about 0.8 mg/kg, about 0.7 mg/kg to about 0.75 mg/kg, about 0.7 mg/kg to about 2.15 mg/kg, about 0.85 mg/kg to about 2 mg/kg, about 1 mg/kg to about 1.85 mg/kg, about 1.15 mg/kg to about 1.7
  • an effective dosage of each of the BMX inhibitors is about 0.35 mg/kg, about 0.7 mg/kg, about 1 mg/kg, about 1.4 mg/kg, about 1.8 mg/kg, about 2.1 mg/kg, about 2.5 mg/kg, about 2.85 mg/kg, about 3.2 mg/kg, or about 3.6 mg/kg.
  • a inhibitor of each of the BMX inhibitors is adminstered at a dosage of 10 to 400 mg BID, including a dosage of 5 mg, 10 mg, 12.5 mg, 25 mg, 50 mg, 75 mg, 100 mg, 150 mg, 175 mg, 200 mg, 225 mg, 250 mg, 275 mg, 300 mg, 325 mg, 350 mg, 375 mg, 400 mg, 425 mg, 450 mg, 475 mg, and 500 mg BID.
  • An effective amount of the BMX inhibitor may be administered in either single or multiple doses by any of the accepted modes of administration of agents having similar utilities, including rectal, buccal, intranasal and transdermal routes, by intra-arterial injection,
  • the effective amount of the BMX inhibitor is in the range of from about 1 mg to about 300 mg, more preferably from about 1 mg to about 200 mg, more preferably from about 1 mg to about 100 mg, and even more preferably from about 1 mg to about 50 mg, on a daily or twice daily basis.
  • the invention relates to a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a mammal that comprises administering to said mammal a therapeutically effective amount of a BMX inhibitor, or a pharmaceutically acceptable salt or ester, prodrug, solvate or hydrate of the BMX inhibitor.
  • the invention relates to a method of treating, with a BMX inhibitor, a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a mammal selected from the group consisting of bladder cancer, head and neck cancer, pancreatic ductal adenocarcinoma (PDA), pancreatic cancer, colon carcinoma, mammary carcinoma, breast cancer, fibrosarcoma, mesothelioma, renal cell carcinoma, lung carcinoma, thyoma, prostate cancer, colorectal cancer, ovarian cancer, acute myeloid leukemia, thymus cancer, brain cancer, squamous cell cancer, skin cancer, eye cancer, retinoblastoma, melanoma, intraocular melanoma, oral cavity and oropharyngeal cancers, gastric cancer, stomach cancer, cervical cancer, head, neck, renal cancer, kidney cancer, liver
  • the invention relates to a method of treating an inflammatory, immune, or autoimmune disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a mammal with a BMX inhibitor.
  • the invention also relates to a method of treating a disease with a BMX inhibitor, wherein the disease is selected from the group consisting of tumor angiogenesis, chronic inflammatory disease, rheumatoid arthritis, atherosclerosis, inflammatory bowel disease, skin diseases such as psoriasis, eczema, and scleroderma, diabetes, diabetic retinopathy, retinopathy of prematurity, age-related macular degeneration, hemangioma, glioma and melanoma, ulcerative colitis, atopic dermatitis, pouchitis, spondylarthritis, uveitis, Behcets disease, polymyalgia rheumatica, giant-cell arteritis, sarcoidosis, Kawasaki disease, juvenile idiopathic arthritis, instaenitis suppurativa, Sjogren's syndrome, psoriatic arthritis, juvenile rheumato
  • the disease
  • the invention relates to a method of treating with a BMX inhibitor a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway including but not limited to acute myeloid leukemia, thymus cancer, brain cancer, lung cancer, squamous cell cancer, skin cancer, eye cancer, retinoblastoma, intraocular melanoma, oral cavity and oropharyngeal cancer, bladder cancer, gastric cancer, stomach cancer, pancreatic cancer, bladder cancer, breast cancer, cervical, head cancer, neck cancer, renal cancer, kidney cancer, liver cancer, ovarian cancer, prostate cancer, colorectal cancer, esophageal cancer, testicular cancer, gynecological cancer, thyroid cancer, and CNS, PNS, AIDS-related (e.g., lymphoma and Kaposi's sarcoma) or viral-induced cancers.
  • CXCR4/SDF-1 signaling pathway
  • said pharmaceutical composition is for the treatment of a non-cancerous hyperproliferative disorder such as benign hyperplasia of the skin (e.g., psoriasis), restenosis, or prostate (e.g., benign prostatic hypertrophy (BPH)).
  • a non-cancerous hyperproliferative disorder such as benign hyperplasia of the skin (e.g., psoriasis), restenosis, or prostate (e.g., benign prostatic hypertrophy (BPH)).
  • Efficacy of the compounds and combinations of compounds described herein in treating, preventing and/or managing the indicated diseases or disorders can be tested using various models known in the art. For example, models for determining efficacy of treatments for overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway are described, e.g., in Bachelerie, Disease Markers, 2010, 29, 189-198. Models for determining efficacy of treatments for pancreatic cancer are described in Herreros-Villanueva, et al, World J. Gastroenterol. 2012, 18, 1286-1294. Models for determining efficacy of treatments for breast cancer are described e.g.
  • Models for determining efficacy of treatments for ovarian cancer are described, e.g., in Mullany, et al, Endocrinology 2012, 153, 1585-92; and Fong et al, J. Ovarian Res. 2009, 2, 12.
  • Models for determining efficacy of treatments for melanoma are described, e.g., in Damsky, et al, Pigment Cell & Melanoma Res. 2010, 23, 853-859.
  • Models for determining efficacy of treatments for lung cancer are described, e.g., in Meu Giveaway, et al, Genes & Development, 2005, 19, 643-664.
  • Models for determining efficacy of treatments for lung cancer are described, e.g., in Kim, Clin. Exp. Otorhinolaryngol. 2009, 2, 55-60; and Sano, Head Neck Oncol. 2009, 1, 32. Models for determining efficacy of treatments for colorectal cancer, including the CT26 model, are described below in the examples.
  • Efficacy of the compounds and combinations of compounds described herein in treating, preventing and/or managing other indicated diseases or disorders described here can also be tested using various models known in the art. Efficacy in treating, preventing and/or managing asthma can be assessed using the ova induced asthma model described, for example, in Lee, et al, J. Allergy Clin. Immunol. 2006, 118, 403-9. Efficacy in treating, preventing and/or managing arthritis (e.g. , rheumatoid or psoriatic arthritis) can be assessed using the autoimmune animal models described in, for example, Williams, et al, Chem. Biol.
  • Efficacy in treating, preventing and/or managing psoriasis can be assessed using transgenic or knockout mouse model with targeted mutations in epidermis, vasculature or immune cells, mouse model resulting from spontaneous mutations, and immuno-deficient mouse model with xenotransplantation of human skin or immune cells, all of which are described, for example, in Boehncke, et al, Clinics in
  • Efficacy in treating, preventing and/or managing fibrosis or fibrotic conditions can be assessed using the unilateral ureteral obstruction model of renal fibrosis, which is described, for example, in Chevalier, et al, Kidney International 2009, 75, 1145-1152; the bleomycin induced model of pulmonary fibrosis described in, for example, Moore, et al, Am. J. Physiol. Lung. Cell. Mol. Physiol. 2008, 294, L152-L160; a variety of liver/biliary fibrosis models described in, for example, Chuang, et al, Clin. Liver Dis.
  • Efficacy in treating, preventing and/or managing dermatomyositis can be assessed using a myositis mouse model induced by immunization with rabbit myosin as described, for example, in Phyanagi, et al, Arthritis & Rheumatism, 2009, 60(10), 3118-3127.
  • Efficacy in treating, preventing and/or managing lupus can be assessed using various animal models described, for example, in Ghoreishi, et al, Lupus, 2009, 19, 1029-1035; Ohl, et al., J. Biomed.
  • the invention provides a method of treating a treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to bleeding events, comprising the step of administering a therapeutically effective dose of a BMX inhibitor or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to bleeding events, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to bleeding events, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), and wherein the disorder is selected from the group consisting of bladder cancer, squamous cell carcinoma including head and neck cancer, pancreatic ductal adenocarcinoma (PDA), pancreatic cancer, colon carcinoma, mammary carcinoma, breast cancer, fibrosarcoma, mesothelioma, renal cell carcinoma, lung carcinoma, thyoma, prostate cancer, colorectal cancer, ovarian cancer, acute myeloid leukemia, thymus cancer, brain cancer, squamous cell cancer, skin cancer, eye cancer, retinoblastoma, melanoma, intra
  • a BMX inhibitor where
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • a BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • a BMX inhibitor wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), or a
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet- mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof, further comprising the step of administering a therapeutically effective dose of an anticoagulent or antiplatelet agent.
  • a BMX inhibitor wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof, further comprising the step of administering a therapeutically effective dose of an anticoagulent or antiplatelet agent.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof, further comprising the step of administering a therapeutically effective dose of an anticoagulent or antiplatelet agent, wherein the anticoagulent or antiplatelet agent is selected from the group consisting of clopidogrel, prasugrel, ticagrelor, ticlopidine, warfarin, acenocoumarol, dicumarol, phenprocoumon, heparain, low molecular weight heparin, fondaparinux, and idraparin
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof, further comprising the step of administering a therapeutically effective dose of an anticoagulent or antiplatelet agent, wherein the anticoagulent or antiplatelet agent is selected from the group consisting of clopidogrel, prasugrel, ticagrelor, ticlopidine, warfarin, acenocoumarol, dicumarol, phenprocoumon, heparain, low molecular weight heparin, fondaparinux, and idraparinux.
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), and wherein the disorder is selected from the group consisting of bladder cancer, squamous cell carcinoma including head and neck cancer, pancreatic ductal adenocarcinoma (PDA), pancreatic cancer, colon carcinoma, mammary carcinoma, breast cancer, fibrosarcoma, mesothelioma, renal cell carcinoma, lung carcinoma, thyoma, prostate cancer, colorectal cancer, ovarian cancer, acute myeloid leukemia, thymus cancer, brain cancer, squamous cell cancer, skin cancer, eye cancer, retinoblastoma,
  • a BMX inhibitor where
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is Formula (II), and wherein the disorder is selected from the group consisting of bladder cancer, squamous cell carcinoma including head and neck cancer, pancreatic ductal adenocarcinoma (PDA), pancreatic cancer, colon carcinoma, mammary carcinoma, breast cancer, fibrosarcoma, mesothelioma, renal cell carcinoma, lung carcinoma, thyoma, prostate cancer, colorectal cancer, ovarian cancer, acute myeloid leukemia, thymus cancer, brain cancer, squamous cell cancer, skin cancer, eye cancer, retinoblastoma,
  • a BMX inhibitor where
  • the BMX inhibitor and the anticoagulent or the antiplatelet active pharmaceutical ingredient are administered sequentially. In selected embodiments, the BMX inhibitor and the anticoagulent or the antiplatelet active pharmaceutical ingredient are administered concomittently. In selected embodiments, the BMX inhibitor is administered before the anticoagulent or the antiplatelet active pharmaceutical ingredient. In selected embodiments, the BMX inhibitor is administered after the anticoagulent or the antiplatelet active pharmaceutical ingredient.
  • Selected anti-platelet and anticoagulent active pharmaceutical ingredients for use in the methods of the present invention include, but are not limited to, cyclooxygenase inhibitors (e.g., aspirin), adenosine diphosphate (ADP) receptor inhibitors (e.g., clopidogrel and ticlopidine), phosphodiesterase inhibitors (e.g., cilostazol), glycoprotein Ilb/IIIa inhibitors (e.g., abciximab, eptifibatide, and tirofiban), adenosine reuptake inhibitors (e.g., dipyridamole), and acetylsalicylic acid (aspirin).
  • cyclooxygenase inhibitors e.g., aspirin
  • ADP adenosine diphosphate
  • phosphodiesterase inhibitors e.g., cilostazol
  • glycoprotein Ilb/IIIa inhibitors e.g., ab
  • examples of anti-platelet active pharmaceutical ingredients for use in the methods of the present invention include anagrelide, aspirin/extended-release dipyridamole, cilostazol, clopidogrel, dipyridamole, prasugrel, ticagrelor, ticlopidine, vorapaxar, tirofiban HC1, eptifibatide, abciximab, argatroban, bivalirudin, dalteparin, desirudin, enoxaparin, fondaparinux, heparin, lepirudin, apixaban, dabigatran etexilate mesylate, rivaroxaban, and warfarin.
  • the invention includes a method of treating a disorder associated with overexpression of CXCR4 or dysregulated signaling of CXCR4, comprising the step of orally administering, to a human in need thereof, a Bruton's tyrosine kinase (BMX) inhibitor, wherein the BMX inhibitor is (5)-4-(8-amino-3-(l-(but-2-ynoyl)pyrrolidin-2-yl)imidazo[l ,5- a]pyrazin-l-yl)-N-(pyridin-2-yl)benzamide or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, further comprising the step of administering a Bruton's tyrosine kinase (BMX) inhibitor, wherein the BMX inhibitor is (5)-4-(8-amino-3-(l-(but-2-ynoyl)pyrrolidin-2-yl)imidazo[l
  • an anticoagulant or antiplatelet active pharmaceutical ingredient wherein the anticoagulant or antiplatelet active pharmaceutical ingredient is selected from the group consisting of acenocoumarol, anagrelide, anagrelide hydrochloride, abciximab, aloxiprin, antithrombin, apixaban, argatroban, aspirin, aspirin with extended-release dipyridamole, beraprost, betrixaban, bivalirudin, carbasalate calcium, cilostazol, clopidogrel, clopidogrel bisulfate, cloricromen, dabigatran etexilate, darexaban, dalteparin, dalteparin sodium, defibrotide, dicumarol, diphenadione, dipyridamole, ditazole, desirudin, edoxaban, enoxaparin, enoxaparin sodium, eptifibatide, fond
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human with a history of thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the invention provides a method of treating a disorder associated with
  • the invention provides a method of treating a disorder associated with overexpression of CXCR4, dysregulated signaling of CXCR4, or pathogenesis mediated by the CXCR4/SDF-1 signaling pathway in a human sensitive to platelet-mediated thrombosis, method of treating a cancer in a human with a history of thrombosis, comprising the step of administering a therapeutically effective dose of a BMX inhibitor, wherein the BMX inhibitor is a compound of Formula (XXVIII) or a pharmaceutically-acceptable salt, cocrystal, hydrate, solvate, or prodrug thereof.
  • the BMX inhibitor and the anticoagulent or the antiplatelet agent are administered sequentially. In selected embodiments, the BMX inhibitor and the anticoagulent or the antiplatelet agent are administered concomittently. In selected
  • the BMX inhibitor is administered before the anticoagulent or the antiplatelet agent. In selected embodiments, the BMX inhibitor is administered after the anticoagulent or the antiplatelet agent.
  • Preferred anti-platelet and anticoagulent agents for use in the methods of the present invention include, but are not limited to, cyclooxygenase inhibitors (e.g., aspirin), adenosine diphosphate (ADP) receptor inhibitors (e.g., clopidogrel and ticlopidine), phosphodiesterase inhibitors (e.g., cilostazol), glycoprotein Ilb/IIIa inhibitors (e.g., abciximab, eptifibatide, and tirofiban), adenosine reuptake inhibitors (e.g., dipyridamole), and acetylsalicylic acid (aspirin).
  • cyclooxygenase inhibitors e.g., aspirin
  • ADP adenosine diphosphate
  • phosphodiesterase inhibitors e.g., cilostazol
  • glycoprotein Ilb/IIIa inhibitors e.g., abcix
  • examples of anti-platelet agents for use in the methods of the present invention include anagrelide, aspirin/extended-release dipyridamole, cilostazol, clopidogrel, dipyridamole, prasugrel, ticagrelor, ticlopidine, vorapaxar, tirofiban HCl, eptifibatide, abciximab, argatroban, bivalirudin, dalteparin, desirudin, enoxaparin, fondaparinux, heparin, lepirudin, apixaban, dabigatran etexilate mesylate, rivaroxaban, and warfarin.
  • Recombinant human SDF-1 can be purchased from ProSpec (East Brunswick, NJ).
  • Antibodies All phospho-specific antibodies can be purchased from BD Bioscience (San Jose, CA) or Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody R- phycoerythrin-conjugated anti-human CXCR4 antibody (clone 12G5) is available from R&D Systems (Minneapolis, MN).
  • Table 2 shows phospho-specific antibodies. Each antibody is conjugated to either Alexa-488 or Alexa 647 fluorophores. TABLE 2. Phospho-specific antibodies.
  • SJSA-1 osteosarcoma
  • U-937 leukemia
  • MOLM13 leukemia
  • T24 blade cancer
  • PC3 prostate cancer
  • mice Female BALB/c mice (6 to 8 weeks old) can be purchased from The Jackson Laboratory (Bar Harbor, ME).
  • CXCR4- SDF1 assay The blocking potential of an agent to inhibit BMX activity is tested using a CXCR4- SDF1 assay.
  • Cancer cells that express high levels of CXCR4 e.g., SJSA-1 (osteosarcoma), U- 973 (leukemia), MOLM13 (leukemia), T24 (bladder cancer), and PC3 (prostate cancer)
  • CXCR4 e.g., SJSA-1 (osteosarcoma), U- 973 (leukemia), MOLM13 (leukemia), T24 (bladder cancer), and PC3 (prostate cancer)
  • SJSA-1 osteosarcoma
  • U- 973 leukemia
  • MOLM13 leukemia
  • T24 bladedder cancer
  • PC3 prostate cancer
  • a separate control sample is incubated with a BMX inhibitor (e.g., ASTX-1304).
  • the signaling reaction is stopped by the addition of 2% paraformalde
  • Cells are permeabilized with ice-cold 90% methanol for 1 hour on ice, and the supernatant removed by centrifugation for 5 minutes at 300XG. Permeabilized cells are resuspended with a PBS 1% BSA solution.
  • the cells are fixed cells in a staining cocktail that includes cell surface (anti-CXCR4 antibody) and phospho-specific antibodies (Table 2) conjugated to specific fluorescent dyes, and mean fluorescence intensity (MFI) obtained by flow cytometric analysis. MFI values are compared across the stimulation time course to calculate a fold-change based from the basal state MFI value (0 minute time point). Any fold change values >1.5 for a specific phospho-protein readout will be regarded as a positive signaling result.
  • a staining cocktail that includes cell surface (anti-CXCR4 antibody) and phospho-specific antibodies (Table 2) conjugated to specific fluorescent dyes, and mean fluorescence intensity (MFI) obtained by flow cytometric analysis. MFI values are compared across the stimulation time course to calculate a fold-change based from the basal state MFI value (0 minute time point). Any fold change values >1.5 for a specific phospho-protein readout will be regarded as a positive signaling result.
  • the results show the signaling nodes activated by SDF-1 at the particular time points in each cancer cell line.
  • BMX activity on the CXCR4/SDF-1 driven signaling network in each CXCR4 + cancer cell line is investigated by including ASTX-1304, or other specific BMX inhibitors, across a range of concentrations.
  • other specific kinase inhibitors such as GDC-0941 (PI3K inhibitor) are tested separately and compared to the results using ASTX-1304.
  • the CXCR4 inhibitor, plerixafor is included as a control for total blockade of SDF-1 induced signaling.
  • CXCR4 osteosarcoma
  • U-973 leukemia
  • MOLM13 leukemia
  • T24 blade cancer
  • PC3 prostate cancer
  • polyvinylpyrrolidone-free polycarbonate filters (Nucleopore, Corning Costar Corp., Cambridge, MA). Briefly, the lower compartment of the chamber is loaded with aliquots of serum-free medium plus rhSDF-1 (100 ng/mL). Before the invasion assay, filters are coated with 100 ⁇ g/well Matrigel (Becton Dickinson) diluted in culture medium. Tumor cells are seeded onto the reconstituted basement for 24 hours (5 x 10 4 cells per well) at 37 °C in humidified atmosphere with 5% C0 2 . Cells that pass the synthetic basement are fixed with ethanol and stained with Giemsa solution (Diff-Quik kit, Baxter Diagnostics, Milan, Italy). Each experiment is performed in triplicate.
  • BMX inhibition On abating invasion is assessed by preincubating (> 30 minutes) the tumor cells with increasing concentrations (10 nM, 100 nM, and ⁇ ⁇ ) of ASTX-1304, or other BMX inhibitors, before seeding them onto the reconstituted basement. Results are expressed as the percentage of migrated cells.
  • Cancer cells (described above) are grown in lOOcc tissue culture plates and treated with increasing concentrations (10 nM, 100 nM, and ⁇ ⁇ ) of ASTX-1304, or other BMX inhibitors, or left untreated overnight. 8-strip wells coated with fibronectin, vitronectin, laminin, collagen type I or collagen type IV (Chemicon, Temecula, CA) is hydrated with 200 ⁇ of PBS for 15 min. Cells are harvested by the addition of Cellstripper (Mediatech, Herndon, VA) solution for 10 min.
  • Cellstripper Mediatech, Herndon, VA
  • ASTX-1304 (or other BMX inhibitor)-treated and untreated cells are centrifuged and resuspended in media to a final concentration of 500,000 cells/ml. After removal of the PBS from the coated 8-well strips, 50,000 cells in a volume of 100 ⁇ are added to each well, incubated for 1 h at 37°C in a tissue culture incubator and the media removed. The wells are washed twice with PBS containing calcium and magnesium. 100 ⁇ of crystal violet solution (0.2% dissolved in 10% ethanol) is added and incubated for 5 min at room temperature.
  • mice are sacrificed 5 weeks after injection and lungs examined macro scopically and microscopically for the presence of metastases.
  • lungs are collected and fixed in 10% formalin and embedded in paraffin. Sections of 4 ⁇ thickness will be stained with H&E for conventional histology.
  • Healthy PBMCs were incubated with and without Formula (II) at the shown concentrations for 2 hours and then stimulated with anti-IgM [10 ug/mL] or anti-IgM + SDF-1 [50 ng/mL] for 5 minutes at 37 °C.
  • Cells were then analyzed by phosphoflow cytometry and p- Btk(Y223) was measured in the CD20+ B cell gate and the CD14 + monocyte gate separately. Results are shown in FIG. 1 and FIG. 2.
  • BTK phosphorylation in anti-IgM stimulated B cells is increased with the addition of SDF-1 (CXCL-12) in the "No drug" condition.
  • the BTK phosphorylation is inhibited by the BMX inhibitor Formula (II) at low nM concentrations in both stimulation conditions. In monocytes, BTK phosphorylation is only observed when SDF-1 is present. Formula (II) at low nM concentrations in both stimulation conditions.
  • FIG. 3 and FIG. 4 show that CXCR4 surface expression is decreased in B-CLL (B cell chronic lymphocytic leukemia) following administration of the BMX inhibitor of Formula (II).
  • CLL patients with the 17p deletion were administered Formula (II) at 100 mg BID (FIG. 3) and 200 mg QD (FIG. 4). Drug was given for 3 days. Blood was drawn predose, and then 4, 24, and 48 hours after the day 3 dose. The results show that in B-CLL cells, CXCR4 surface expression in samples after Formula (II) administration is reduced compared to the predose levels.
  • FIG. 5 and FIG. 6 illustrate that BMX inhibition is not impacting the ability of CD34 cells to migrate towards SDF-1 (CXCL-12) in both fresh and cryopreserved CD34 + cells.
  • FIG. 7 illustrates that BMX inhibition is not impacting the ability of U937 cells to migrate towards SDF-1 (CXCL-12).
  • FIG. 8 illustrates that BMX inhibition is not impacting the ability of CLL cells to migrate towards SDF-1 (CXCL-12).
  • FIG. 9 illustrates the results of a RANK MDA-MB231 mouse experiment.
  • the primary endpoint was
  • SDF-1 binds to CXCR4 and amplifies B cell receptor-induced BMX phosphorylation.
  • the contribution of SDF-1 in amplifying the BMX pathway in B cells may contribute to a pro- survival phenotype. This may be especially important in the anti-apoptotic phenotype of malignant cells (i.e., CLL B cells).
  • BMX inhibition by Formula (II) inhibits the SDF-1/CXCR4 signaling in B cells.
  • Monocytes also express CXCR4 and express BMX.
  • SDF-1 induced p-Btk can be measured by phosphoflow cytometry.
  • BMX inhibition by Formula (II) inhibits the SDF-1/CXCR4 signaling in monocytes.
  • BMX inhibition by itself, does not appear to inhibit in vitro cell migration towards SDF-1, suggesting that non-BMX pathways may be more relevant for cell migration.
  • BMX inhibition over a period of days results in decreased expression of CXCR4 in some patients, which would likely limit the migration potential towards SDF-1 and possibly the prosurvival signals as well.

Abstract

Dans certains modes de réalisation, cette invention concerne de nouveaux inhibiteurs à petites molécules qui bloquent la voie de signalisation des CXCR4-SDF-1 par inhibition directe des membres de la famille des kinases Tec, à savoir la kinase X de moelle osseuse (BMX) (également connue sous le nom de tyrosine kinase épithéliale et endothéliale (ETK)), et leur utilisation pour traiter les maladies chez lesquelles la pathogenèse est médiée par la voie de signalisation des CXCR4-SDF-1.
PCT/IB2015/001425 2014-04-11 2015-04-10 Procédés de blocage de la voie de signalisation des cxcr-4/sdf-1 à l'aide d'inhibiteurs de kinase x de moelle osseuse WO2015185998A2 (fr)

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CN108137605A (zh) * 2016-10-05 2018-06-08 杭州领业医药科技有限公司 Acp-196的晶型及其制备方法和药物组合物
US10899767B2 (en) 2016-10-05 2021-01-26 Hangzhou Solipharma Co., Ltd. Crystal form of ACP-196, preparation method therefor and pharmaceutical composition thereof
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