WO2015029595A1 - 核酸分析装置およびその装置診断方法 - Google Patents
核酸分析装置およびその装置診断方法 Download PDFInfo
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- WO2015029595A1 WO2015029595A1 PCT/JP2014/067774 JP2014067774W WO2015029595A1 WO 2015029595 A1 WO2015029595 A1 WO 2015029595A1 JP 2014067774 W JP2014067774 W JP 2014067774W WO 2015029595 A1 WO2015029595 A1 WO 2015029595A1
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 125
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 124
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- 238000004458 analytical method Methods 0.000 title claims abstract description 82
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
- G01J1/44—Electric circuits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
- G01J1/44—Electric circuits
- G01J2001/444—Compensating; Calibrating, e.g. dark current, temperature drift, noise reduction or baseline correction; Adjusting
Definitions
- the present invention relates to a nucleic acid analysis device and a device diagnostic method thereof, for example, a nucleic acid analysis device for analyzing a biological sample by amplifying a nucleic acid contained in a biological sample and a device diagnostic method thereof.
- Patent Document 1 discloses an analysis disc and an inspection method of the analysis apparatus in an analyzer which irradiates light to the analysis disc and detects its reflected light and transmitted light.
- the presence or absence of defects in the analysis disc and the analysis device is determined based on the detection result of the transmitted light without the analysis disc attached and the detection result of the reflected light and the transmitted light with the analysis disc attached. It is determined.
- Patent Document 2 an excitation LED for irradiating excitation light to an object to be detected, a light receiving unit for receiving fluorescence generated by the object to be detected, and excitation light are monitored so that the intensity of excitation light becomes constant.
- a fluorescence detection device is shown that includes a mechanism for providing feedback to the excitation LED.
- Patent Document 3 shows a method of determining the degree of deterioration of a photodiode array in a spectrometric analysis apparatus that disperses measurement light and measures the light quantity of each wavelength with a photodiode array. In this method, after measuring the dark current and temperature of the photodiode array, the degree of deterioration is determined by comparing the dark current value with the allowable current value corrected according to the temperature.
- Patent No. 4525427 gazette JP 2012-37355 A JP 2004-37192 A
- PCR Polymerase Chain Reaction
- a reaction solution in which a reagent etc. is mixed with a nucleic acid to be analyzed is analyzed immediately after preparation.
- the reason for this is to first avoid the deterioration of the nucleic acid to be analyzed and the enzyme contained in the reagent with the passage of time.
- the nucleic acid and the reagent are prepared, there is a need to minimize the possibility of nonspecific annealing or extension reaction different from the desired nucleic acid sequence.
- the analyst launches the nucleic acid analyzer. Since some nucleic acid analyzers may not be able to start analysis immediately after start-up, the devices may be started before preparation.
- the nucleic acid analyzer emits light to the sample, receives light (fluorescent light) emitted from the sample, and performs analysis.
- important parts that carry out the nucleic acid analysis device ie, LED (Light Emitting Diode) serving as a light source, semiconductor laser, PD (Photodiode) serving as a detector, CCD (Charge Coupled Device), etc. If degraded, the correct analysis can not be performed. Especially in the medical field, errors in analysis results can be fatal. Therefore, the nucleic acid analyzer is required to detect and warn of failure or performance deterioration of these important parts at an early stage.
- the sample may not be wasted by keeping it refrigerated.
- the nucleic acid analyzer detect a failure or performance deterioration, for example, while the device is up and waiting for analysis (in other words, before starting a PCR reaction). At this time, in order to shorten the analysis waiting time and improve the throughput of the apparatus, it is required that the detection time for failure or performance deterioration be short. Furthermore, it is desirable to be able to detect which parts have broken so that repairs can be done quickly.
- Patent Document 1 it is possible to use the techniques of Patent Document 1 to Patent Document 3.
- the technique of Patent Document 1 it is possible to detect an abnormality of the measurement system before actual sample measurement.
- a component for example, a light source or a detector
- the technique of Patent Document 1 since the process of attaching and detaching the analysis disc is required, there is a risk that it may take time to detect an abnormality in the measurement system. That is, the throughput of the device may be reduced.
- the present invention has been made in view of the foregoing, and one of the objects thereof is to provide a nucleic acid analyzer capable of rapidly detecting an abnormality or the like of the device and a device diagnostic method therefor. is there.
- the nucleic acid analysis device has a holding member for holding a reaction container containing a sample, a photometer, and a device diagnosis unit.
- the photometer includes a light source for emitting light toward the holding member in a state in which the reaction container is held, and a first detector for receiving light emitted from the sample in response to the irradiation of light from the light source.
- the apparatus diagnosis unit performs, as the process related to the present embodiment, a first process of causing the light source to emit light toward the holding member in a state in which the reaction container is not held, and the first detector according to the first process. And a third process of diagnosing the photometer based on the intensity of the scattered light detected by the first detector.
- FIG. 3 is a schematic block diagram showing a main configuration example in its functional aspect in the nucleic acid analysis device of FIG. 1 and FIG. 2; It is a flowchart which shows an example of the processing content of the apparatus diagnostic part in FIG. FIG.
- FIG. 7 is a diagram showing an example of temporal transition of detection signals obtained by an excitation light monitor detector and a fluorescence detector in the device diagnosis flow of FIG. 6.
- FIG. 7 is a diagram showing an example of a temporal transition of detection signals obtained by the excitation light monitor detector and the fluorescence detector when the fluorescence detector fails in the device diagnosis flow of FIG. 6.
- FIG. 7 is a diagram summarizing one example of the relationship between a combination of detection signals from an excitation light monitor detector and a fluorescence detector and an abnormality of a component that can be determined from the combination in the device diagnosis flow of FIG. 6. It is a figure following FIG. 9A. It is a figure following FIG. 9B. It is a figure following FIG. 9C.
- FIG. 9A It is a figure following FIG. 9B.
- FIG. 9C It is a figure following FIG. 9C.
- FIG. 8 is an explanatory view schematically showing an example of a device diagnosis method of the nucleic acid analysis device according to the second embodiment of the present invention.
- FIG. 17 is a diagram showing an example of temporal transition of detection signals obtained by the excitation light monitor detector and the fluorescence detector in the nucleic acid analysis device according to the third embodiment of the present invention.
- the nucleic acid analyzer according to the fourth embodiment of the present invention is a top view showing a schematic configuration example thereof.
- the constituent elements are not necessarily essential unless explicitly stated or considered to be obviously essential in principle. Needless to say.
- the shapes, positional relationships and the like of components etc. when referring to the shapes, positional relationships and the like of components etc., the shapes thereof are substantially the same unless particularly clearly stated and where it is apparently clearly not so in principle. It is assumed that it includes things that are similar or similar to etc. The same applies to the above numerical values and ranges.
- FIG. 1 is a top view showing a configuration example of a main part in a nucleic acid analysis device according to a first embodiment of the present invention.
- FIG. 2 is a cross-sectional view showing a configuration example between AA ′ of FIG.
- a plurality (12 in this example) of temperature control blocks 1 are arranged along the outer periphery around the central axis of the carousel 2, and rotates around the rotation axis 3. It is driven.
- Peltier elements 4 are disposed between the plurality of temperature control blocks 1 and the carousel 2 respectively.
- the temperature of the temperature control block 1 is adjusted by controlling the Peltier element 4 while monitoring the temperature with the temperature sensor 5 mounted in the temperature control block 1.
- the temperatures of the plurality of temperature control blocks 1 are adjusted independently of each other.
- a photometer 6 is disposed around the carousel 2.
- two photometers 6 using light of different wavelengths are shown as an example, but one or three or more photometers 6 may be disposed as long as the outer periphery of the carousel 2 is used. Since all the temperature control blocks 1 move on the same circumference by rotational drive, the relative positions of the photometer 6 and the temperature control block 1 when passing in front of the light meter 6 are the same in all the temperature control blocks 1 become.
- the plurality of temperature control blocks 1 are covered with a shielding plate 7 including the carousel 2 in order to reduce optical disturbance when analyzed by the photometer 6.
- a temperature control block (holding member) 1 holds a tube (reaction container) 10 including a reaction liquid (sample) in which reagents and the like are mixed with nucleic acid. All the temperature control blocks 1 are provided with an excitation light irradiation window 8 for receiving excitation light from the light meter 6 and a fluorescence detection window 9 for the light meter 6 to take in fluorescence.
- the excitation light irradiation window 8 is disposed on the lower surface side of the temperature control block 1 and the fluorescence detection window 9 is disposed on the side surface side of the temperature adjustment block 1, the arrangement of the windows is freely according to the structure of the photometer. It is possible to set.
- FIG. 3 is a schematic view showing a detailed configuration example of a photometer in the nucleic acid analyzer of FIG. 1 and FIG.
- excitation light emitted from an LED (Light Emitting Diode) 11 as a light source passes through the lens 12 to become parallel light, and passes through the excitation light filter (band pass filter) 13. Only the necessary wavelength components are extracted. Part of the light that has passed through the excitation light filter 13 is reflected by the half mirror 14 and is incident on the excitation light monitor detector (second detector) 15.
- the excitation light monitor detector 15 is configured of, for example, a photoelectric conversion diode (PD: Photodiode) or the like.
- PD photoelectric conversion diode
- the remaining light that has passed through the half mirror 14 is collected by the lens 16 and enters the excitation light irradiation window 8 of the temperature control block (holding member) 1.
- a tube (reaction container) 10 containing a reaction liquid (sample) in which a reagent etc. is mixed with nucleic acid is held.
- the reaction liquid in the tube 10 emits fluorescence in response to the excitation light.
- the fluorescence emitted from the fluorescence detection window 9 of the temperature control block 1 becomes parallel light again by the lens 17, passes through the fluorescence filter (band pass filter) 18, and only necessary wavelength components are extracted.
- the light passing through the fluorescent filter 18 is collected by the lens 19 and is incident on the fluorescent detector (first detector) 20.
- the fluorescence detector 20 is configured of, for example, a photoelectric conversion diode (PD) or the like.
- the LED 11 which is a light source always emits excitation light
- the excitation light monitor detector 15 and the fluorescence detector 20 always perform detection.
- the excitation light monitor detector 15 and the fluorescence detector 20 generate a detection signal (current or voltage) according to the intensity of the light, and the detection signal is A / D converted through a signal amplification circuit to a signal processing circuit. It is transmitted.
- the load on the nucleic acid analyzer 31 is large, so the nucleic acid analyzer 31 actually applies a trigger just before the temperature control block 1 passes in front of the photometer 6. Immediately after passing, control of stopping acquisition of the detection signal is performed.
- a detection signal as shown in FIG. 4 is typically obtained.
- FIG. 4 is a diagram showing an example of temporal transition of detection signals obtained when analyzing nucleic acid in the excitation light monitor detector and the fluorescence detector of FIG. 3.
- the detection signal by the excitation light monitor detector 15 exhibits a substantially constant value.
- the detection signal by the fluorescence detector 20 becomes a signal having a mountain-like waveform over time, and reaches a peak at the moment when the center line of the temperature control block 1 to be measured passes the optical axis of the LED 11 of the photometer 6 . Since the signal contains electrical noise, many nucleic acid analyzers curve fit the waveform of the detection signal according to a certain rule to obtain an approximate curve, obtain the peak value of the approximate curve, and change the value Nucleic acid analysis is performed by observation.
- FIG. 5 is a schematic block diagram showing a main configuration example of the functional aspect in the nucleic acid analysis device of FIG. 1 and FIG.
- the nucleic acid analyzer 31 shown in FIG. 5 includes, in addition to the plurality of temperature control blocks 1, the carousel 2, and the photometer 6 described above, an analysis processing unit 36 that performs control and the like.
- the analysis processing unit 36 mainly includes a computer system or the like, and performs temperature adjustment of each temperature control block 1, rotation control of the carousel 2, control of the photometer 6, and the like based on a predetermined processing sequence. Further, the analysis processing unit 36 processes the signal obtained by each detector in the photometer 6.
- a device diagnosis unit 37 is included in the analysis processing unit 36.
- FIG. 6 is a flow chart showing an example of processing contents of the device diagnosis unit in FIG.
- the device diagnosis unit 37 is activated, for example, immediately after the power of the nucleic acid analyzer 31 is turned on, and executes the process of FIG.
- the apparatus diagnosis unit 37 installs or passes the temperature control block (holding member) 1 in a state in which the tube (reaction container) 10 shown in FIG. 3 is not held on the photometer 6 through the control of the carousel 2.
- Step S101 the device diagnostic unit 37 causes the LED (light source) 11 in the photometer 6 to emit excitation light toward the temperature control block 1 (step S102).
- the light source is unstable immediately after lighting, so it may be irradiated in advance.
- the LED (light source) 11 may be irradiated in advance.
- the device diagnosis unit 37 causes the excitation light monitor detector (second detector) 15 to detect the intensity of the excitation light associated with step S102 (step S103). Subsequently, the device diagnosis unit 37 causes the fluorescence detector (first detector) 20 to detect the intensity of the scattered light generated in step S102 (step S104).
- the scattered light can be generated not only at the temperature control block 1 made of, for example, aluminum etc. but also at various other places. For example, as shown in FIG. 2, since the temperature control block 1 is covered by the shielding plate 7 including the carousel 2, scattered light is generated at various places in the space covered by the shielding plate 7. , And enters the fluorescence detector 20 through the fluorescence detection window 9.
- the device diagnosis unit 37 diagnoses the presence or absence of abnormality (fault, performance deterioration) of the photometer 6 based on the intensity of the excitation light detected in step S103 and the intensity of the scattered light detected in step S104. (Step S105).
- Step S105 a specific method of diagnosing the presence or absence of an abnormality of the photometer 6 in step S105 will be described.
- FIG. 7 is a diagram showing an example of temporal transition of detection signals obtained by the excitation light monitor detector and the fluorescence detector in the apparatus diagnosis flow of FIG.
- the detection signal from the excitation light monitor detector 15 exhibits a substantially constant value.
- the detection signal from the fluorescence detector 20 is observed although weak. This is because when the temperature adjustment block 1 is irradiated with the excitation light, a part thereof is scattered and enters the fluorescence detector 20.
- the diagnosis is performed in the state where the light emission power of the LED 11 is set to the same level as in the case of FIG. It is also possible to make a diagnosis in a state of increasing the scattered light and increasing the
- FIG. 8 is a diagram showing an example of temporal transition of detection signals obtained by the excitation light monitor detector and the fluorescence detector when the fluorescence detector fails in the device diagnosis flow of FIG.
- the detection signal from the excitation light monitor detector 15 exhibits a substantially constant value.
- the detection signal from the fluorescence detector 20 is weaker than in the case of FIG. This weak signal is an electrical noise or an external optical disturbance.
- the fluorescence detector 15 It becomes possible to detect 20 abnormalities. Similarly, if a detection signal from the excitation light monitor detector 15 is provided with a threshold in advance and an alarm is issued if the detection signal falls below the threshold, an abnormality in the excitation light monitor detector 15 is detected. It becomes possible.
- FIG. 9A to 9D are diagrams summarizing one example of the relationship between the combination of detection signals from the excitation light monitor detector and the fluorescence detector in the device diagnosis flow of FIG. .
- FIG. 9A schematically shows FIG.
- the intensity of excitation light represented by the level of the excitation light monitor detection signal is higher than the intensity (second reference value) of the reference represented by the predetermined excitation light monitor signal threshold, and the intensity of scattered light represented by the fluorescence detection signal is Higher than the reference intensity (first reference value) represented by the defined fluorescence detection signal threshold.
- the device diagnosis unit 37 diagnoses that the LED 11, the excitation light monitor detector 15, and the fluorescence detector 20 are all normal.
- FIG. 9B is a schematic representation of FIG.
- the intensity of the excitation light is higher than the intensity of the reference (second reference value), and the intensity of the scattered light is lower than the intensity of the reference (first reference value).
- the device diagnosis unit 37 determines that the LED 11 and the excitation light monitor detector 15 have no abnormality.
- the intensity of the scattered light is lower than the reference intensity (first reference value) despite the fact that the excitation light is output normally, the device diagnosis unit 37 diagnoses the fluorescence detector 20 as abnormal. Do.
- the intensity of the scattered light is higher than the intensity of the reference (first reference value)
- the intensity of the excitation light is lower than the intensity of the reference (second reference value).
- the fact that the intensity of the scattered light is higher than the reference intensity (first reference value) means that the operation of the fluorescence detector 20 is normal and the scattered light is normally incident on the fluorescence detector 20. , Means that the LED 11 is on. Therefore, in this case, the device diagnosis unit 37 diagnoses the excitation light monitor detector 15 as abnormal.
- the intensity of excitation light is lower than the intensity of the reference (second reference value), and the intensity of scattered light is also lower than the intensity of the reference (first reference value).
- Case 1 LED 11 is abnormal (Whether the excitation light monitor detector 15 and the fluorescence detector 20 are normal or abnormal is unknown)
- Case 2 LED 11 is normal and both the excitation light monitor detector 15 and the fluorescence detector 20 are There are two possible causes. Generally, it is considered that the probability that two elements will fail simultaneously is extremely small. In this case, the device diagnosis unit 37 diagnoses the LED 11 as abnormal.
- the first reference value and the second reference value described above do not necessarily have to be fixed values, and may be changed as necessary.
- the diagnosis of the device can be performed at the time of Further, by detecting both the excitation light and the scattered light with the irradiation of the excitation light, it becomes possible to specify a component that causes an apparatus abnormality unlike the cases of Patent Document 1 to Patent Document 3. Furthermore, in the device diagnosis, the advanced monitor function as in Patent Document 3 is not particularly required, and the device diagnosis can be performed using the parts that can usually be mounted on the nucleic acid analysis device as it is. .
- a system may be considered in which the apparatus is diagnosed using a tube including a standard sample or the like for abnormality detection in a diagnosis period of the apparatus immediately after the power is turned on.
- the nucleic acid analyzer it is not always easy to create a standard sample itself, and even if a standard sample is created, processing such as insertion and discharge of a tube is required.
- processing such as insertion and discharge of a tube becomes unnecessary, so that the diagnosis period can be shortened and the cost associated with the preparation of a standard sample can be reduced.
- the ejected normal sample may be automatically discarded from the viewpoint of the safety of the apparatus.
- the apparatus is diagnosed by detecting the excitation light and the scattered light with respect to one temperature control block 1 in a state where the tube 10 is not present.
- the intensity of the scattered light ie, the detection signal of the fluorescence detector 20
- the scattered light is a slight shape difference of each part
- FIG. 10 is an explanatory view schematically showing an example of a device diagnostic method of the nucleic acid analysis device according to the second embodiment of the present invention.
- the excitation light and the scattered light are detected by the photometer 6 for each of the 12 temperature control blocks 1 System to diagnose the
- the device diagnosis unit 37 of FIG. 5 controls rotation of the carousel 2 such that the twelve temperature control blocks 1 sequentially pass the photometer 6, while the temperature control block 1 of FIG.
- the processes of steps S102 to S104 are performed.
- the process of step S103 is only for less than 12 (for example, one) temperature control block 1 May be targeted.
- the apparatus diagnostic unit 37 predetermines 12 detection signals from the fluorescence detector 20 and 12 or less detection signals from the excitation light monitor detector 15 obtained in steps S102 to S104. This compares the diagnostic criteria with which to diagnose the device.
- the device diagnostic unit 37 has 12 excitation light intensity detected by the excitation light monitor detector 15 higher than the intensity of the reference (second reference value) and 12 fluorescence detectors 20 If all the intensities of the scattered light corresponding to the detection signals are lower than the reference intensity (first reference value), the fluorescence detector 20 is determined to be abnormal.
- the diagnostic criteria are not necessarily limited to this.
- an abnormality may be judged by the average value of 12 detection signals, and the intensity of the reference (first reference value) is lower than 12 out of 12 detection signals. It does not matter if you judge by the ratio. Further, it is not necessary to use all 12 detection signals from the fluorescence detector 20, and it may be determined by a plurality of which some are omitted.
- FIG. 11 is a diagram showing an example of temporal transition of detection signals obtained by the excitation light monitor detector and the fluorescence detector in the nucleic acid analysis device according to the third embodiment of the present invention.
- maintain the empty tube 10 is shown by FIG.
- the detection signal from the excitation light monitor detector 15 exhibits a substantially constant value.
- the detection signal from the fluorescence detector 20 has a peak waveform over time. This is because the presence of the tube 10 increases the scattered light. In general, if a reagent or the like is contained in the tube 10, the signal level is further increased by the influence.
- the threshold of the detection signal from the fluorescence detector 20 is set to a threshold higher than the initial threshold (that is, the state where the tube 10 is not in the temperature control block 1).
- the initial threshold that is, the state where the tube 10 is not in the temperature control block 1.
- the fluorescence detection signal can be at the same level as that shown in FIG. There is a case.
- the detection signal from the excitation light monitor detector 15 does not change even before the start of the analysis or during the analysis, so it is possible to diagnose the device under analysis without setting the threshold separately. It is.
- the fluorescence detector 20 after the analysis is started, it is desirable to avoid making a diagnosis from the waveform of one detection signal as in the case of the second embodiment. Therefore, in the third embodiment as well, as in the second embodiment, for example, a plurality of detection signals from the fluorescence detector 20 are acquired while sequentially passing a plurality of temperature control blocks 1 to the photometer 6 It is desirable to determine the abnormality of the fluorescence detector 20 based on predetermined diagnostic criteria.
- the method of performing device diagnosis in a preparation period waiting for analysis is performed, and in the third embodiment, device diagnosis is performed during analysis.
- the device diagnosis may be performed during the preparation period waiting for the next analysis after the analysis as in the first and second embodiments.
- device diagnosis may be performed before the power is turned off.
- FIG. 12 is a top view showing a schematic configuration example of the nucleic acid analysis device according to the fourth embodiment of the present invention.
- the nucleic acid analyzer 32 shown in FIG. 12 includes a nucleic acid extraction unit 33 for extracting nucleic acid from a sample, a reagent mixing unit 34 for dispensing and mixing a reagent with the extracted nucleic acid, and temperature control of the reaction solution after mixing. And a nucleic acid analysis unit 35 for detecting
- the nucleic acid extraction unit 33 includes a sample setting unit 41, a centrifugal unit 42, a evacuation chamber 43, a tube setting unit 44, an extraction reagent storage 45, a consumable storage 46, etc. It is responsible for removing components and extracting only nucleic acids necessary for analysis.
- the reagent mixing unit 34 includes an analysis reagent storage 47, a consumable storage 48, a mixing unit 49 and the like, and although a detailed description is omitted, a reagent for analysis is mixed with the nucleic acid extracted by the nucleic acid extraction unit 33. Take on the function.
- the configuration of the nucleic acid analysis unit 35 is the same as that of the nucleic acid analysis device 31 shown in FIG. 1 and has a function of analyzing the nucleic acid to be the final step. The transfer of the tubes between the units is performed by the robot arm 50.
- the person who executes the analysis starts up the nucleic acid analyzer 32, sets consumables such as samples, reagents, tubes and the like, and starts the analysis.
- the light source and detector are at the stage of starting the apparatus (ie, the preparation stage of the apparatus immediately after power on). It is possible to detect early abnormality (fault, performance deterioration) of the photometer 6 including the When the photometer 6 is normal, the nucleic acid analyzer 32 shifts to the normal operation of the device and starts pretreatment for analysis by the nucleic acid extraction unit 33 and the reagent mixing unit 34.
- the photometer 6 can be repaired, and no sample is wasted.
- the nucleic acid extraction unit 33, the reagent mixing unit 34, and the nucleic acid analysis unit 35 are respectively configured by separate devices, it may occur that mixing of reagents has already been performed.
- the diagnosis of the device can be performed in a short time immediately after the power supply of the device is turned on. It becomes possible to raise.
- the present invention is not limited to the above-mentioned embodiment, and can be variously changed in the range which does not deviate from the gist.
- the above-described embodiments are described in detail in order to explain the present invention in an easy-to-understand manner, and are not necessarily limited to those having all the described configurations.
- part of the configuration of one embodiment can be replaced with the configuration of another embodiment, and the configuration of another embodiment can be added to the configuration of one embodiment. .
- the nucleic acid analyzer which is a particularly useful application example of the device diagnostic method according to the present embodiment has been described.
- the present invention is not necessarily limited to the nucleic acid analysis device, and any device that sets a reaction container to a holding member and analyzes a sample in the reaction container using a photometer is similarly applied, and the same effect is obtained. May be obtained.
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Abstract
Description
《核酸分析装置の主要部の構成》
図1は、本発明の実施の形態1による核酸分析装置において、その主要部の構成例を示す上面図である。図2は、図1のA-A’間の構成例を示す断面図である。図1および図2の核酸分析装置31において、温調ブロック1は、カローセル2の中心軸周りで外周に沿って複数個(この例では12個)配置されており、回転軸3を中心に回転駆動される。複数の温調ブロック1とカローセル2との間にはそれぞれペルチェ素子4が配置される。温調ブロック1の温度は、温調ブロック1内に搭載された温度センサ5で温度をモニタしながらペルチェ素子4を制御することで調整される。複数の温調ブロック1のそれぞれに対応してペルチェ素子4及び温度センサ5を一組ずつ配置することで、複数の温調ブロック1の温度は、それぞれ独立に調整される。
図3は、図1および図2の核酸分析装置における光度計の詳細な構成例を示す模式図である。図3の光度計6において、光源であるLED(Light Emitting Diode、発光ダイオード)11から照射された励起光は、レンズ12を通過して平行光となり、励起光フィルタ(バンドパスフィルタ)13を通過して必要な波長成分だけが取り出される。励起光フィルタ13を通過した一部の光は、ハーフミラー14で反射し、励起光モニタ検出器(第2検出器)15へ入射する。励起光モニタ検出器15は、例えば、光電変換用ダイオード(PD:Photodiode)等で構成される。
次に、以上のように構成した核酸分析装置において、光源や検出器の異常(故障、性能劣化)を検出するための装置診断方法について説明する。
以上、本実施の形態1の核酸分析装置を用いることで、装置の異常等を迅速に検出することが可能となり、その原因となる部品(光源なのか検出器なのか)を特定することが可能になる。すなわち、例えば特許文献1のように、部材の着脱等の処理を経ることなく、単に、チューブ10が保持されない状態の温調ブロック1に対して励起光を照射し、その散乱光を検出することで装置の診断が行えるため、短い期間で診断を行うことができる。また、励起光の照射に伴いその励起光と散乱光を共に検出することで、特許文献1~特許文献3の場合と異なり、装置異常の原因となる部品を特定することが可能になる。さらに、装置診断に際して、特許文献3のような高度なモニタ機能は特に必要とされず、核酸分析装置に通常搭載され得る部品をそのまま用いて装置の診断が行えるため、コストの観点でも有益となる。
《装置診断方法の詳細(応用例)》
前述した実施の形態1では、チューブ10が無い状態の1個の温調ブロック1を対象に励起光および散乱光を検出することで、装置の診断を行った。ただし、実際には、温調ブロック1にチューブ10が無い状態での散乱光の強度(すなわち蛍光検出器20の検出信号)は微弱であり、また、散乱光は、各部品のわずかな形状差などによっても増減する恐れがある。したがって、部品の異常という重要な判断を、1個の温調ブロック1を対象とした診断結果から下すことは、誤診断のリスクを高める場合がある。
以上、本実施の形態2の核酸分析装置を用いることで、部品の異常という重要な判断の信頼性を上げることができ、誤診断のリスクを低くすることが可能になる。
《装置診断方法の詳細(変形例)》
前述した実施の形態1および2で述べた装置診断方法は、温調ブロック1にチューブ10が入っていない状態で行えるため、例えば、核酸分析装置の電源投入後、分析を待機している準備期間にて容易にかつ短時間で実施することができる。一方で、光度計6(すなわち光源や検出器)の異常は、準備期間の後の核酸の分析中(すなわち通常動作時)にも起こり得る。分析中に異常が起きた場合は、その分析結果が正しくない可能性を示す必要があり、やはり装置の異常を検出することが必要である。
《核酸分析装置の構成》
図12は、本発明の実施の形態4による核酸分析装置において、その概略的な構成例を示す上面図である。図12の核酸分析装置32は、検体から核酸を抽出する核酸抽出ユニット33と、抽出した核酸に試薬を分注し、混合する試薬混合ユニット34と、混合後の反応液を温調して蛍光を検出する核酸分析ユニット35とを備える。
2 カローセル
3 回転軸
4 ペルチェ素子
5 温度センサ
6 光度計
7 遮蔽板
8 励起光照射窓
9 蛍光検出窓
10 チューブ
11 LED
12,16,17,19 レンズ
13 励起光フィルタ
14 ハーフミラー
15 励起光モニタ検出器(第2検出器)
18 蛍光フィルタ
20 蛍光検出器(第1検出器)
31,32 核酸分析装置
33 核酸抽出ユニット
34 試薬混合ユニット
35 核酸分析ユニット
36 分析処理部
37 装置診断部
41 検体架設部
42 遠心部
43 退避室
44 チューブ架設部
45 抽出試薬保管庫
46,48 消耗品保管庫
47 分析試薬保管庫
49 混合部
50 ロボットアーム
Claims (15)
- 試料が含まれる反応容器を保持する保持部材と、光度計と、装置診断部と、を有する核酸分析装置であって、
前記光度計は、
前記反応容器が保持された状態の前記保持部材に向けて光を照射する光源と、
前記光源からの光の照射に応じて前記試料から放射される光を受光する第1検出器と、
を備え、
前記装置診断部は、
前記光源に、前記反応容器が保持されない状態の前記保持部材に向けて光を照射させる第1処理と、
前記第1検出器に、前記第1処理に応じて生じる散乱光を検出させる第2処理と、
前記第1検出器で検出された前記散乱光の強度に基づいて前記光度計の診断を行う第3処理と、
を実行する、核酸分析装置。 - 請求項1記載の核酸分析装置において、
前記光度計は、さらに、前記光源から照射された光の強度を検出する第2検出器を備え、
前記装置診断部は、前記第2処理の際に、前記第2検出器に、前記光源から照射された光の強度を検出させ、前記第3処理の際に、前記第1検出器で検出された前記散乱光の強度と前記第2検出器で検出された前記光の強度とに基づいて前記光度計の診断を行う、核酸分析装置。 - 請求項2記載の核酸分析装置において、
前記装置診断部は、前記第3処理の際に、
前記第1検出器で検出された前記散乱光の強度が予め定めた第1基準値よりも低く、かつ前記第2検出器で検出された前記光の強度が予め定めた第2基準値よりも高い場合には、前記第1検出器を異常と診断し、
前記第1検出器で検出された前記散乱光の強度が前記第1基準値よりも高く、かつ前記第2検出器で検出された前記光の強度が前記第2基準値よりも低い場合には、前記第2検出器を異常と診断する、核酸分析装置。 - 請求項3記載の核酸分析装置において、
前記装置診断部は、前記第3処理の際に、前記第1検出器で検出された前記散乱光の強度が前記第1基準値よりも低く、かつ前記第2検出器で検出された前記光の強度が前記第2基準値よりも低い場合には、前記光源を異常と診断する、核酸分析装置。 - 請求項3記載の核酸分析装置において、
前記保持部材は、複数設けられ、
前記装置診断部は、前記複数の保持部材のそれぞれに対して前記第1および第2処理を実行し、前記第3処理の際に、前記複数の保持部材毎の前記第1および第2検出器での検出結果を予め定めた診断基準と比較することで前記光度計の診断を行う、核酸分析装置。 - 請求項5記載の核酸分析装置において、
前記複数の保持部材は、それぞれ独立に温度調整が可能な機構を備える、核酸分析装置。 - 反応容器内で核酸に試薬を混合して試料を作成する試薬混合ユニットと、
前記試料を分析する分析ユニットと、
を有する核酸分析装置であって、
前記分析ユニットは、前記反応容器を保持する保持部材と、光度計と、前記核酸分析装置の電源投入直後に起動される装置診断部と、を有し、
前記光度計は、
前記反応容器が保持された状態の前記保持部材に向けて光を照射する光源と、
前記光源からの光の照射に応じて前記試料から放射される光を受光する第1検出器と、
を備え、
前記装置診断部は、
前記光源に、前記反応容器が保持されない状態の前記保持部材に向けて光を照射させる第1処理と、
前記第1検出器に、前記第1処理に応じて生じる散乱光を検出させる第2処理と、
前記第1検出器で検出された前記散乱光の強度に基づいて前記光度計の診断を行う第3処理と、
前記第3処理の診断結果が正常であった場合に前記試薬混合ユニットの処理を開始させる第4処理と、
を実行する、核酸分析装置。 - 請求項7記載の核酸分析装置において、
前記光度計は、さらに、前記光源から照射された光の強度を検出する第2検出器を備え、
前記装置診断部は、前記第2処理の際に、前記第2検出器に、前記光源から照射された光の強度を検出させ、前記第3処理の際に、前記第1検出器で検出された前記散乱光の強度と前記第2検出器で検出された前記光の強度とに基づいて前記光度計の診断を行う、核酸分析装置。 - 請求項8記載の核酸分析装置において、
前記装置診断部は、前記第3処理の際に、
前記第1検出器で検出された前記散乱光の強度が予め定めた第1基準値よりも低く、かつ前記第2検出器で検出された前記光の強度が予め定めた第2基準値よりも高い場合には、前記第1検出器を異常と診断し、
前記第1検出器で検出された前記散乱光の強度が前記第1基準値よりも高く、かつ前記第2検出器で検出された前記光の強度が前記第2基準値よりも低い場合には、前記第2検出器を異常と診断する、核酸分析装置。 - 請求項9記載の核酸分析装置において、
前記装置診断部は、前記第3処理の際に、前記第1検出器で検出された前記散乱光の強度が前記第1基準値よりも低く、かつ前記第2検出器で検出された前記光の強度が前記第2基準値よりも低い場合には、前記光源を異常と診断する、核酸分析装置。 - 試料が含まれる反応容器を保持する保持部材と、
前記反応容器が保持された状態の前記保持部材に向けて光を照射する光源、および前記光源からの光の照射に応じて前記試料から放射される光を受光する第1検出器を備える光度計と、
を有する核酸分析装置の装置診断方法であって、
前記光源が、前記反応容器が保持されない状態の前記保持部材に向けて光を照射する第1工程と、
前記第1検出器が、前記第1工程に応じて生じる散乱光を検出する第2工程と、
前記核酸分析装置が、前記第1検出器で検出された前記散乱光の強度に基づいて前記光度計の診断を行う第3工程と、
を有する、核酸分析装置の装置診断方法。 - 請求項11記載の核酸分析装置の装置診断方法において、
前記光度計は、さらに、前記光源から照射された光の強度を検出する第2検出器を備え、
前記第2工程の際に、前記第2検出器は、前記光源から照射された光の強度を検出し、
前記第3工程の際に、前記核酸分析装置は、前記第1検出器で検出された前記散乱光の強度と前記第2検出器で検出された前記光の強度とに基づいて前記光度計の診断を行う、核酸分析装置の装置診断方法。 - 請求項12記載の核酸分析装置の装置診断方法において、
前記核酸分析装置は、前記第3工程の際に、
前記第1検出器で検出された前記散乱光の強度が予め定めた第1基準値よりも低く、かつ前記第2検出器で検出された前記光の強度が予め定めた第2基準値よりも高い場合には、前記第1検出器を異常と診断し、
前記第1検出器で検出された前記散乱光の強度が前記第1基準値よりも高く、かつ前記第2検出器で検出された前記光の強度が前記第2基準値よりも低い場合には、前記第2検出器を異常と診断する、核酸分析装置の装置診断方法。 - 請求項13記載の核酸分析装置の装置診断方法において、
前記核酸分析装置は、前記第3工程の際に、前記第1検出器で検出された前記散乱光の強度が前記第1基準値よりも低く、かつ前記第2検出器で検出された前記光の強度が前記第2基準値よりも低い場合には、前記光源を異常と診断する、核酸分析装置の装置診断方法。 - 請求項13記載の核酸分析装置の装置診断方法において、
前記保持部材は、複数設けられ、
前記第1および第2工程は、前記複数の保持部材のそれぞれに対して行われ、
前記核酸分析装置は、前記第3工程の際に、前記複数の保持部材毎の前記第1および第2検出器での検出結果を予め定めた診断基準と比較することで前記光度計の診断を行う、核酸分析装置の装置診断方法。
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Also Published As
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DE112014003484B4 (de) | 2023-03-02 |
CN105518439A (zh) | 2016-04-20 |
DE112014003484T5 (de) | 2016-04-14 |
US9857219B2 (en) | 2018-01-02 |
JPWO2015029595A1 (ja) | 2017-03-02 |
CN105518439B (zh) | 2018-02-16 |
US20160245690A1 (en) | 2016-08-25 |
JP6130917B2 (ja) | 2017-05-17 |
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