WO2011013728A1 - 経粘膜吸収性を付与したモチリン類似ペプチド化合物 - Google Patents
経粘膜吸収性を付与したモチリン類似ペプチド化合物 Download PDFInfo
- Publication number
- WO2011013728A1 WO2011013728A1 PCT/JP2010/062746 JP2010062746W WO2011013728A1 WO 2011013728 A1 WO2011013728 A1 WO 2011013728A1 JP 2010062746 W JP2010062746 W JP 2010062746W WO 2011013728 A1 WO2011013728 A1 WO 2011013728A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- motilin
- compound
- seq
- natural
- amino acid
- Prior art date
Links
- 0 *CN* Chemical compound *CN* 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/63—Motilins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Definitions
- the present invention relates to functional dyspepsia, diabetic gastric paresis, gastroesophageal reflux disease, irritable bowel syndrome, small intestinal bacterial hyperproliferation, colonic pseudoobstruction, paralytic intestinal obstruction, chronic idiopathic pseudointestinal obstruction, and surgery
- the present invention relates to a motilin-like peptide compound having a gastrointestinal motility stimulating activity useful for the treatment of symptoms characterized by gastrointestinal dysfunction such as post-intestinal obstruction and a high absorbability upon transmucosal administration.
- Motilin is a bioactive peptide consisting of 22 amino acids isolated from intestinal smooth muscle, and its structure was first revealed in 1973 by the isolation of porcine motilin (Non-patent Documents 1 and 2). In 1995, human motilin was isolated and identified, and was found to have the same structure as porcine motilin (Non-patent Document 3).
- IMC Interdigestive-Migrating Complex
- IMC is a type of physiological function that plays a role in cleaning the digestive tract by flushing the digestive tract contents such as epithelium, mucous membrane, and secretions that have fallen off the digestive tract into the lower part of the digestive tract.
- motilin is secreted from the duodenum and jejunum approximately 100 minutes in the fasting period, and IMC occurs with the increase in plasma motilin concentration (Non-patent Document 4), and motilin is administered to dogs, monkeys, and humans.
- Non-patent Documents 5, 6, and 7 When administered, IMC is induced (Non-patent Documents 5, 6, and 7), and the administration of anti-motilin serum suppresses physiological IMC generation. It is considered that digestive tract functions such as food digestion and digestive juice secretion are maintained normally by removing the residue (Non-patent Document 8).
- Non-patent Document 9 Non-patent Document 9
- Non-patent Document 13 There is a report that the occurrence of IMC is not reduced or observed in diseases with functional dysepsia, gastrointestinal dysfunction such as postoperative ileus and chronic idiopathic ileus.
- Non-patent Document 14 There is a report (Non-patent Document 14) that a decrease in the amount of endogenous motilin secreted, a decrease in motilin action, and an abnormality / decrease in gastrointestinal motility function.
- Irritable bowel syndrome has chronic abdominal discomfort such as abdominal pain and bloating due to lower gastrointestinal dysfunction mainly in the large intestine, although there are no basic diseases such as inflammation and tumor, such as constipation and diarrhea It is a chronic intestinal disorder that causes bowel movements.
- Non-patent Document 9 Intestinal accumulation of residues in the gastrointestinal tract is thought to be one of the causes of changes in the intestinal flora.
- the current first-line drugs are oral drugs for improving gastrointestinal motility such as dopamine receptor antagonists, selective serotonin 5-HT4 agonists, parasympathomimetic drugs and the like.
- dopamine receptor antagonists selective serotonin 5-HT4 agonists
- parasympathomimetic drugs and the like.
- treatment with these therapeutic agents may show temporary improvement in symptoms, there are many cases in which the therapeutic effect is not observed, and doctors and patients are less satisfied with current drugs for improving gastrointestinal motility function is the current situation. Therefore, the provision of a therapeutic drug based on a new mechanism of action is eagerly desired, and normalizing gastrointestinal function by administering a motilin receptor agonist from the outside to patients with gastrointestinal dysfunction, Expected to relieve symptoms or cure disease.
- Non-Patent Documents 19-20
- Non-Patent Documents 21 and 22 there are several oral agents that have been used in clinical trials.
- HERG inhibition, spermatogenesis, carcinogenicity unrelated to the action as motilin agonists, or the reduction of drug efficacy by repeated administration.
- Non-patent Documents 15 and 16 Clinical trials using natural motilin or peptidic motilin agonists have been carried out on patients with gastrointestinal dysfunction as peptide compounds.
- Non-patent Documents 15 and 16 Clinical trials and animal experiments using these peptide compounds, only intravenous administration has been reported as a route of administration, and long-term repeated administration to patients is difficult, and therapeutic effects have not been confirmed.
- Atilmotin (Patent Document 1) was developed by Baxter, and SK-896 (Patent Document 2) was developed by Sanwa Chemical.
- the former is a compound created for the purpose of improving the half-life in vivo, and is metabolically stable by metabolic experiments using the homogenate supernatant of the kidney (Non-patent Document 17), which is considered to be the main metabolic organ of motilin. Improvement has been confirmed (Patent Document 1), and the plasma half-life is about 10 minutes, which is about 3 times longer than that of motilin (Non-Patent Document 18).
- the latter is a compound designed for the purpose of improving production efficiency. In addition, both maintain gastrointestinal motility enhancing activity equivalent to motilin.
- any compound is similar to motilin in that the route of administration is limited to intravenous administration, and is limited to treatment in a medical facility and cannot be used for a long time. For this reason, drug discovery based on the action of maintaining normal gastrointestinal function by removing residues in the gastrointestinal tract inherent to motilin has not been achieved, and its therapeutic effect has not been confirmed.
- An object of the present invention is to provide a motilin-like peptide compound that maintains the gastrointestinal motility stimulating activity of natural type motilin and is given high absorbability in transmucosal administration.
- motilin would be degraded by non-invasive transmucosal administration, and conducted a motilin degradation experiment using the lung homogenate supernatant. It was. As a result, it was revealed that motilin was degraded, and by identifying the degradation product, it was presumed that the C-terminal was degraded by dicarboxypeptidase as a degradation pathway of motilin.
- the present inventors substituted the glycine at position 21 with proline, thereby inhibiting degradation by C-terminal dicarboxypeptidase and exhibiting high absorbability in transmucosal administration.
- the present invention has been completed.
- phenylalanine at the N-terminal is subject to degradation, so that the amide bond between the first amino acid and the second amino acid at the N-terminal is a non-peptide bond described below -psi [CH 2 NH] -bonding has also been shown to contribute to further improved metabolic stability.
- the present invention specifically includes the following formula (1) wherein the amino acid at position 21 is characterized by proline substitution: X1 Val X2 Ile Phe Thr Tyr Gly X3 Leu Gln Arg X4 Gln Glu Lys Glu Arg X5 Lys Pro Gln (Formula 1) (SEQ ID NO: 2) [In the formula, the bonds between amino acids are all amide bonds except for the bond between X1 and Val; X1 is an aromatic or heteroaromatic amino acid; The bond between X1 and Val is an amide bond or the following formula 2 A bond represented by: X2 is proline or sarcosine; X3 is glutamic acid or aspartic acid; X4 is methionine or leucine; X5 is asparagine or proline)
- a peptide compound comprising a sequence represented by or a pharmaceutically acceptable salt thereof and having both gastrointestinal motility stimulating activity equivalent to natural motilin and high absorbability in transmucosal
- X1 in (Formula 1) is ⁇ -amino acid such as phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp), ⁇ -homophenylglycine (Phg (C # CH 2 )), acetylnaphthyl
- X1 in (Formula 1) is phenylalanine or ⁇ -homophenylglycine.
- (Iv) A compound represented by a sequence selected from the group consisting of SEQ ID NO: 3 to 15, or a pharmaceutically acceptable salt thereof.
- V A compound represented by SEQ ID NO: 4, or a pharmaceutically acceptable salt thereof.
- Vi A compound represented by SEQ ID NO: 6, or a pharmaceutically acceptable salt thereof.
- Vii A compound represented by SEQ ID NO: 8, or a pharmaceutically acceptable salt thereof.
- Viii A pharmaceutical composition for treating a disease associated with abnormal gastrointestinal function, comprising the peptide compound according to any one of (i) to (vii) or a pharmaceutically acceptable salt thereof.
- the novel motilin-like peptide compound according to the present invention has motilin-like gastrointestinal motility stimulating activity and exhibits high absorption efficiency in transmucosal administration. Accordingly, the compounds of the present invention can be used to treat diseases associated with gastrointestinal dysfunction (eg, symptoms characterized by a reduction in basal levels of gastrointestinal motility activity). Diseases with gastrointestinal dysfunction include functional dyspepsia, diabetic gastric paresis, gastroesophageal reflux disease, irritable bowel syndrome, small intestinal bacterial hyperproliferation, colonic pseudoobstruction, paralytic intestinal obstruction, chronic idiopathic Symptoms such as pseudo intestinal obstruction and postoperative intestinal obstruction can be mentioned. Moreover, the absorption efficiency by transmucosal administration is higher than that of natural type motilin, and it is possible to achieve a plasma concentration that is effective for treatment of patients at a lower dose.
- FIG. 1 is a graph showing fasting phase IMC-inducing activity when natural motilin, compound 4 and compound 6 are administered intravenously.
- FIG. 2 is a graph showing changes in plasma concentration after intravenous administration of natural motilin to rats.
- FIG. 3 is a graph showing changes in plasma concentrations after natural pulmonary administration of natural motilin, compound 2 (MT114) and compound 6 (MT140).
- FIG. 4 is a graph showing changes in plasma concentration after intravenous administration of natural motilin and compound 2 (MT114) to monkeys.
- FIG. 5 is a graph showing changes in plasma concentration after nasal administration of natural motilin and compound 2 (MT114) to monkeys.
- Natural type motilin has an N-terminal part essential for activity expression, and natural type motilin forms an ⁇ -helix structure in solution from the vicinity of threonine at the 6th position to the C-terminal side [Andersson A. & Maler L., J Biomol. NMR, 24, 103-112 (2002)], it is also known that a derivative having a mutation introduced into the ⁇ helix structure outside the active center reduces the receptor activation ability [Miller P et al. , Peptides, 16, 11-18 (1995)]. Based on the findings presented by these documents, the present inventors presume that the ⁇ helix structure contributes to the stabilization of the active center at the N-terminus. That is, it was considered that the active center structure at the N-terminus was protected by the presence of the ⁇ helix structure.
- a site that does not affect the decrease in activity associated with the destabilization of the ⁇ -helix structure was selected in the structural modification that blocks the degradation pathway of motilin.
- X2 is a proline in natural type motilin and regulates the three-dimensional structure of the compound, we thought that it may be possible to control the receptor activation ability by increasing the degree of freedom.
- X3 is glutamic acid in natural motilin and is thought to contribute to the formation of an ⁇ -helical structure from the 6th position to the C-terminal side by forming a hydrogen bond between the 6th position threonine and the side chain [AnderssonersA. & Maler L , J. Biomol. NMR, 24, 103-112 (2002)]. It is known that when X3 is substituted with alanine or D-type glutamic acid, the receptor activation ability is known to be greatly reduced [Miller P.
- X3 is preferably selected from L-type acidic amino acids.
- X4 is methionine in natural motilin, and its receptor activation ability is reduced by side chain oxidation. Therefore, substitution with leucine having a side chain size almost equal to that of methionine is desirable.
- Motilin is degraded by dicarboxypeptidase from the C-terminal side. Replacing the amino acid on the C-terminal side of natural motilin with proline fixes the dihedral angle of the peptide bond and acquires degradation resistance due to a three-dimensional structural hindrance when coordinated to the substrate binding site or active center of dicarboxypeptidase I thought.
- the substitution site for proline which is difficult to form the ⁇ -helical structure, is considered to be limited. From the above viewpoints, the proline substitution at the X5 and / or 21 position was conceived.
- the present inventors designed and synthesized the derivatives, and conducted a decomposition experiment using the lung homogenate supernatant and a contraction experiment using the isolated intestinal tract.
- a series of motilin-like peptide compounds of the present invention in which glycine at position 21 of natural type motilin is substituted with proline exhibits high absorbability as compared with natural type motilin in a transmucosal administration experiment in vivo. It was confirmed that IMC-inducing activity similar to that of natural motilin was maintained, and the present invention was completed.
- the peptide compound of the present invention has the following general formula (1), wherein the amino acid at position 21 is characterized by proline substitution: X1 Val X2 Ile Phe Thr Tyr Gly X3 Leu Gln Arg X4 Gln Glu Lys Glu Arg X5 Lys Pro Gln (Formula 1) (SEQ ID NO: 2) [In the formula, the bonds between amino acids are all amide bonds except the bond between X1-Val; The bond between X1 and Val is an amide bond or the following formula 2 A bond represented by: X1 is an aromatic or heteroaromatic amino acid; X2 is proline or sarcosine; X3 is glutamic acid or aspartic acid; X4 is methionine or leucine; X5 is asparagine or proline) Or a pharmaceutically acceptable salt thereof, and has characteristics of having both gastrointestinal motility stimulating activity similar to that of natural type motilin and high absorbability in transmucosal administration.
- X1 is an aromatic amino acid or a heteroaromatic amino acid.
- aromatic amino acids or heteroaromatic amino acids refer to any amino acid containing an aromatic ring, for example, ⁇ -amino acids such as phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp).
- ⁇ -amino acids such as phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp).
- ⁇ -homophenylglycine Phg (C # CH 2 )
- acetylnaphthylalanine Ac-Nal
- phenylalanine, ⁇ -homophenylglycine (Phg (C # CH 2 )), and acetylnaphthylalanine (Ac-Nal) can be mentioned as more preferred examples of the amino acid at the X1 position.
- X1 may be an L-type amino acid or a D-type amino acid.
- a mutant in which an L-type amino acid is substituted with a D-type amino acid has been prepared so far (Miller P. et al., Peptides, 16, 11-18 (1995 )), And its activity is shown to be maintained.
- X2 is proline (Pro) or sarcosine (also called Sar, N-methylglycine, MeGly);
- X3 is glutamic acid (Glu) or aspartic acid (Asp);
- X4 is methionine ( Met) or leucine (Leu); and
- X5 is asparagine (Asn) or proline (Pro).
- the bond between the first amino acid (X1) and the second amino acid (Val) is an amide bond or the following formula 2 Is a combination of
- the bond of Formula 2 is a bond also referred to as a -psi [CH 2 NH]-bond.
- the bond between X1 and Val may be either an amide bond or a -psi [CH 2 NH] -bond regardless of the type of amino acid constituting X1.
- the above-mentioned compound of formula 1 of the present invention includes, for example, a compound represented by the following sequence, or a pharmaceutically acceptable salt thereof: Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Pro Gln (SEQ ID NO: 3); Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Pro Gln (SEQ ID NO: 4); Phe Val Pro Ile Phe Thr Tyr Gly Asp Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln (SEQ ID NO: 5); Phg (C # CH 2 ) Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln (SEQ ID NO: 6); Phg (C # CH 2) Val
- the compound according to the present invention can be obtained by a conventional method.
- a peptide consisting only of chemical synthesis or an L-type natural amino acid it can be produced by recombinant DNA technology or a combination thereof.
- peptides Various methods for chemical synthesis of peptides have already been established, and the compound of the present invention can also be easily produced by known methods.
- a classical peptide synthesis method or a solid phase method can be used.
- an amino acid with a protecting group is condensed by a liquid phase method and / or a solid phase method to extend the peptide chain, and if necessary, the N-terminal protecting group is cleaved with a base such as piperazine and then completely removed with an acid.
- the peptide of the present invention is obtained by removing the protecting group / resin and purifying the resulting crude product using a separation and purification method such as gel filtration, ultrafiltration, dialysis, SDS-PAGE, and various chromatography. You can also.
- a compound having a -psi [CH 2 NH]-bond represented by (Formula 2) as a bond between amino acids can also be obtained by chemical synthesis.
- the peptide chain is synthesized by the above method from the C-terminal to the 2nd amino acid (Val in Formula 1).
- ⁇ ⁇ ⁇ ⁇ Peptides consisting only of L-type natural amino acids can be produced by recombinant DNA technology. Specifically, a host cell transformed with an expression vector having a DNA encoding the peptide sequence of the present invention can be cultured, and the target peptide can be collected from the culture.
- Examples of the vector into which the gene is incorporated include E. coli vectors (pBR322, pUC18, pUC19, etc.), Bacillus subtilis vectors (pUB110, pTP5, pC194, etc.), yeast vectors (YEp type, YRp type, YIp type), or animal cells. These vectors (retrovirus, vaccinia virus, etc.) can be used, and any other vector can be used as long as it can stably hold the target gene in the host cell.
- the vector is introduced into a suitable host cell.
- a method for incorporating a target gene into a plasmid or a method for introducing it into a host cell for example, the method described in Molecular® Cloning (Sambrook et al., 1989) can be used.
- a promoter is connected upstream of the gene so as to function.
- the promoter used in the present invention may be any promoter as long as it is appropriate for the host cell used for expression of the target gene.
- the host cell to be transformed is Escherichia genus
- lac promoter, trp promoter, lpp promoter, ⁇ PL promoter, recA promoter, etc. can be used
- Bacillus genus is used, SPO1 promoter, SPO2 promoter, penP promoter, etc. are used.
- GAP promoter, PHO5 promoter, ADH promoter and the like can be used, and in the case of animal cells, SV40 promoter, CMV promoter, retrovirus-derived promoter and the like can be used.
- the host cell When a host cell is transformed using the vector containing the target gene obtained as described above, the host cell may be a bacterium (eg, Escherichia genus, Bacillus genus, etc.), yeast (Saccharomyces genus, Pichia genus, Candida Genus etc.), animal cells (CHO cells, COS cells etc.), etc. can be used.
- a liquid medium is suitable as a medium for culturing, and it is particularly preferable that the medium contains a carbon source, a nitrogen source, and the like necessary for the growth of the transformed cells to be cultured. If desired, vitamins, growth promoting factors, serum and the like can be added.
- the same separation and purification method as in the case of obtaining the peptide compound by chemical synthesis can be used.
- the compounds of the present invention can be used to treat diseases associated with gastrointestinal dysfunction (for example, symptoms characterized by a decrease in the basic level of gastrointestinal motility activity).
- Gastrointestinal dysfunction is a condition that shows uncomfortable symptoms of the gastrointestinal tract despite the absence of obvious organic abnormalities such as inflammation and ulcers in endoscopy and blood tests.
- the ROMEIII standard (Gastroenterology 130: 1377-1556, 2006)
- the disease group which is collectively called functional gastrointestinal disorders, is classified and defined in detail according to its symptoms.
- Diseases with gastrointestinal dysfunction include functional dyspepsia, diabetic gastric paresis, gastroesophageal reflux disease, irritable bowel syndrome, small intestinal bacterial hyperproliferation, colonic pseudoobstruction, paralytic intestinal obstruction, chronic idiopathic Symptoms such as pseudo intestinal obstruction and postoperative intestinal obstruction can be mentioned.
- gastrointestinal dysfunction includes abdominal pain, nausea, vomiting, diarrhea, constipation, heartburn, abdominal bloating, loss of appetite, gastrointestinal pressure measurement, gastric juice test, gastric pH monitoring, gastric emptying test Diagnosed by gastrointestinal X-ray examination, endoscopy.
- the compound of the present invention or a pharmaceutically acceptable salt thereof may be used as it is or in a known amount of a pharmaceutically acceptable carrier, excipient, bulking agent, etc., in an amount sufficient to give the desired gastrointestinal motility stimulating activity. And can be used for animals including humans.
- salts include, for example, salts with inorganic bases, salts with organic bases, salts with inorganic acids, salts with organic acids, salts with basic or acidic amino acids, and the like.
- the salt is not limited to the above as long as it is a commonly used salt.
- the peptide compound according to the present invention can also be administered by an injection route such as subcutaneous injection, intramuscular injection, intravenous injection or the like. Further, since the peptide compound according to the present invention gives a high absorption amount in transmucosal administration, the administration route is through the mucosa, that is, oral, nasal, pulmonary, oral mucosa, transvaginal, eye drops, etc.
- the non-injection route can be used. Desirable are oral, nasal and pulmonary administration, and most desirably pulmonary and nasal administration.
- the dosage varies depending on the patient's condition, the target therapeutic effect, the route of administration, the number of administrations, etc., and the dosage to humans is as low as 0.01 ⁇ g / kg. Can be changed.
- a preferable dose is 0.01 to 500 ⁇ g / kg, more preferably 0.05 to 100 ⁇ g / kg. It is desirable to administer this amount 1 to 3 times per day.
- the peptide compound of the present invention or a pharmaceutically acceptable salt thereof can be used in combination with a pharmaceutically acceptable carrier.
- a pharmaceutically acceptable carrier various organic or inorganic carrier substances commonly used as pharmaceutical materials are used. Excipients, lubricants, binders, disintegrants in solid preparations; solvents, dissolution aids in liquid preparations , Suspending agents, tonicity agents, buffers, preservatives, antioxidants and the like.
- a typical example of the excipient is lactose, and a typical example of the lubricant is talc or colloidal silica.
- Typical examples of the solvent include water for injection, propylene glycol, sesame oil, and corn oil, and suspending agents such as stearyltriethanolamine, sodium lauryl sulfate, laurylaminopropionic acid, lecithin, glyceryl monostearate, etc.
- suspending agents such as stearyltriethanolamine, sodium lauryl sulfate, laurylaminopropionic acid, lecithin, glyceryl monostearate, etc.
- active agents but other than these may be used as long as they are commonly used pharmaceutical materials.
- Fmoc Fluorenylmethyloxycarbonyl Boc: t-Butyloxycarbonyl TFA: trifluoroacetic acid
- Trt Trityl HBTU: N-[(1H-benzotriazol-1-yl (dimethylamino) methylene] -N-methylmethanaminium hexafluorophosphate N-oxide HOBt: 1-hydroxybenzotriazole
- DCC N, N'-dicyclohexylcarbodiimide
- TIPS Triisopropylsilane.
- the peptide chain was extended mainly by using a peptide synthesizer (433A, Applied Biosystems), constructing a protected peptide derivative-resin by the Fmoc method, and finally introducing a Boc-amino acid at the N-terminus.
- the obtained protected peptide resin was deprotected with trifluoroacetic acid (TFA) or diluted TFA containing various scavengers, and the released peptide was subjected to purification. While purifying by reverse phase HPLC using a C18 column, the purity was confirmed, and the structure was confirmed by mass spectrometry.
- the peptide compound of the present invention can be synthesized by an ordinary peptide synthesis method.
- an ordinary peptide synthesis method examples of synthesis of compound 1 (MT095) and compound 6 (MT140) are shown below.
- Example 1 Synthesis of Compound 1 (MT095) This example was performed for the purpose of chemically synthesizing Compound 1 (MT095, SEQ ID NO: 3).
- Fmoc-Gln (Trt) -Alko resin (Watanabe Chemical Co., Ltd., 47.6 mg, 0.03 mmol) was treated with 20% piperazine for 20 minutes, then SEQ ID NO: Fmoc- by HBTU / HOBt sequentially from the C-terminal side.
- Fmoc-peptide-resin was constructed by repeating amino acid introduction and piperazine de-Fmoc.
- Boc-Phe-OH with DCC / HOBt add 90% trifluoroacetic acid, other phenol, water, and TIPS-containing deprotection reagent (5.0 mL) to the resulting protected peptide resin at room temperature. Stir for 2 hours. The resin was removed by filtration, the filtrate was concentrated, and ether was added to the residue to precipitate. The precipitate was collected by filtration and dried to obtain about 30 mg of crude peptide.
- the obtained target product was added to an Inertsil PREP ODS column ( ⁇ 4.6 mm ⁇ 250 mm, manufactured by GL Sciences Inc.), and a linear gradient (flow rate: 30%) from 0.1% to 65% acetonitrile in 0.1% trifluoroacetic acid.
- the purity of the target product was measured by elution at 1.5 mL / min) and monitoring with UV (220 nm).
- the obtained target product was confirmed by MALDI ⁇ TOF-MS (Daltonics BIFLEX III, Bruker) to confirm the molecular weight and amino acid analysis (hydrolysis to amino acid by treatment at 110 ° C in 6 N-HCl for 24 hours, The peptide content was confirmed by quantifying each amino acid by HPLC.
- MALDI-TOF MS measured value: 2738.718 (theoretical value 2739.2), analytical HPLC purity 96.0%, peptide content by amino acid analysis: 643 ⁇ g / mg.
- Example 2 Synthesis of Compound 6 (MT140) This example was performed for the purpose of chemically synthesizing Compound 6 (MT140, SEQ ID NO: 8).
- Fmoc-Gln (Trt) -Alko resin (Watanabe Chemical Co., Ltd., 47.6 mg, 0.03 mmol) was treated with 20% piperazine for 20 minutes, then SEQ ID NO: Fmoc- by HBTU / HOBt sequentially from the C-terminal side.
- Fmoc-peptide-resin was constructed by repeating amino acid introduction and piperazine de-Fmoc.
- Boc-Phe aldehyde with sodium cyanotrihydroborate / 1% acetic acid the obtained protected peptide resin was treated with 90% trifluoroacetic acid, other phenol, water, and TIPS deprotection reagent (5.0 mL). ) And stirred at room temperature for 2 hours.
- the resin was removed by filtration, the filtrate was concentrated, and ether was added to the residue to form a precipitate.
- the precipitate was collected by filtration and dried to obtain about 30 mg of crude peptide.
- the obtained target product was added to an Inertsil PREP ODS column ( ⁇ 4.6 mm ⁇ 250 mm, manufactured by GL Sciences Inc.), and a linear gradient (flow rate: 30%) from 0.1% to 65% acetonitrile in 0.1% trifluoroacetic acid.
- the purity of the target product was measured by elution at 1.5 mL / min) and monitoring with UV (220 nm).
- the molecular weight of the obtained target product was confirmed by MALDI® TOF-MS® (Daltonics® BIFLEX® III, manufactured by Bruker).
- MALDI-TOF MS measured value: 275.040 (theoretical value 2725.2), analytical HPLC purity 98.7%, peptide content by amino acid analysis: 707 ⁇ g / mg.
- Example 3 Measurement of fasting IMC-inducing activity This example uses the compounds prepared by the methods described in Examples 1 and 2, using the compound prepared by the method described in Examples 1 and 2, anal fast-transmitting strong contraction zone ( IMC) was performed for the purpose of measuring the inducing activity.
- IMC strong contraction zone
- the fasting phase IMC-inducing activity of the compounds was determined by the ITOH et al. Method (Zen ⁇ Itoh et al, Gastroenterologia Japonica 12; 275-283, 1977). This was done by measuring exercise. That is, under anesthesia, the operation of attaching a force transducer along the circular muscle to the serosal surface of the dog's gastric antrum, duodenum, jejunum, and ileum is output to a recorder via an amplifier. did.
- Fig. 1 shows the diagram of fasting IMC-inducing activity when intravenous administration of 0.1 ⁇ g / kg of natural motilin, compound 4 (MT124) and compound 6 (MT140), respectively, and Table 2 shows natural motilin and motilin. The presence or absence of fasting IMC-inducing activity of the derivative compound was shown.
- the arrows indicate the timing of administration of natural motilin and compounds 4 and 6.
- FIG. 1 when natural type motilin is administered (at the time indicated by “ ⁇ ” in the figure), contraction at the anterior floor of the stomach starts and the contraction continues for about 10 minutes.
- Comparative Example 1 Pharmacokinetics experiment of natural type motilin by intravenous administration using rats Natural type motilin was intravenously administered to rats and the plasma concentration was measured.
- Intravenous administration was performed using rats in which a polyethylene tube (PE-50, manufactured by Clay Adams) was previously inserted into the femoral artery.
- a polyethylene tube PE-50, manufactured by Clay Adams
- three 7-week-old male SD rats manufactured by Charles River Japan
- Dissolve natural motilin in 5% mannitol solution to prepare a 100 ⁇ g / mL solution.
- Use this solution at a dose of 1 ⁇ mL / kg, and insert a syringe and 26G needle (both from Terumo) from the tail vein.
- Administered Blood was collected from a polyethylene tube inserted into the femoral artery before administration and 1, 3, 5, 10, 20, 30, and 60 minutes after administration.
- the collected blood was immediately added with 1/100 volume of 10% EDTA ⁇ 2Na ⁇ 2H 2 O solution and centrifuged to separate the plasma. Immediately 1/10 volume of 5,000 IU / mL aprotinin solution was added to the plasma, mixed, and stored at ⁇ 80 ° C. until measurement.
- the plasma concentration of natural motilin was measured by a radioimmunoassay (RIA) method using an anti-motilin antibody. That is, after adding an anti-motilin antibody to a plasma sample, [ 125 I-Tyr7] motilin was added to cause a competitive reaction. A secondary antibody was added thereto to precipitate the motilin bound to the anti-motilin antibody, and after separation of the supernatant, the radioactivity in the precipitated fraction was measured with a ⁇ -counter (Perkin Elmer).
- the resulting plasma natural motilin concentration transition is shown in FIG. Moreover, from the obtained plasma natural motilin concentration transition, the plasma concentration at time 0 (C0) and the area under the plasma concentration curve (AUC) were calculated as pharmacokinetic parameters. C0 was obtained by extrapolation, and AUC was obtained by trapezoidal method. As a result, when natural type motilin was intravenously administered at a dose of 100 ⁇ g / kg, C0 was calculated to be 1797 ng / mL, and AUC was calculated to be 3598 ng ⁇ min / mL.
- Example 4 Pharmacokinetic Experiment of Natural Motilin and Motilin Derivative Compound by Pulmonary Administration in Rats
- natural motilin and motilin derivative compounds were administered to rats by pulmonary administration, and then the plasma concentration of The aim was to measure changes using the RIA method.
- the RIA method is used, all substances showing an antigen-antibody reaction with the anti-motilin antibody are detected, so that there is a possibility of detecting a metabolite that reacts with the antibody in addition to the unchanged substance.
- natural motilin and motilin derivative compounds are transpulmonary administered to rats, and the resulting plasma is fractionated by HPLC and the immunoreactivity in each fraction is measured, so that the substance that reacts with the antibody is unchanged. It was clarified that the RIA method was suitable as a method for measuring changes in plasma concentration.
- a polyethylene tube (PE-240, made by Clay Adams) was inserted into the trachea of a 7-week-old male SD rat (made by Charles River Japan), and natural motilin or compound 2 (MT114) was added to 0.1N N acetic acid.
- a 1 mg / mL solution was prepared by dissolving in an aqueous solution, and 25 ⁇ L of this solution was administered into a tracheal polyethylene tube using an endotracheal liquid spray device (MicroSprayer: manufactured by Penn Century). Five minutes after administration, blood was collected from the abdominal aorta, and the collected blood was treated in the same manner as in Comparative Example 1 to separate plasma.
- the collected plasma was pretreated with a Sep-Pak C18 cartridge (Waters), and then the sample was injected into an HPLC (LC-10A, Takashimazu Corp.) equipped with a Cosmosil 5C18 column (Nacalai Tesque). Fractionation was performed. For each sample, the eluate was collected every minute from immediately after sample injection to 40 minutes, and the immunoreactivity in each fraction was measured by the RIA method. As a result, in any of the plasma samples, an immune activity peak was detected only at the elution position corresponding to the administered compound (unchanged), and no other peak was observed. From the above results, it was suggested that the compound detected in plasma after transpulmonary administration was an unchanged compound and the possibility of the presence of metabolites was very small.
- the RIA method was shown to be suitable as a method.
- the pulmonary administration of the sample was also performed using rats in which a polyethylene tube had been inserted into the femoral artery in the same manner as described above.
- rats in which a polyethylene tube had been inserted into the femoral artery in the same manner as described above.
- three male SD rats manufactured by Charles River Japan Co., Ltd.
- a natural motilin or motilin derivative compound is dissolved in a 0.1 N aqueous acetic acid solution to prepare a 1 mg / mL solution, and 25 ⁇ L of this solution is used for tracheal polyethylene using an intratracheal liquid spray device (MicroSprayer: manufactured by Penn Century). It was administered into the tube.
- Blood was collected from a polyethylene tube inserted into the femoral artery before administration and 5, 10, 20, 30 and 60 minutes after administration.
- the collected blood was treated in the same manner as in Comparative Example 1 to separate plasma, and the plasma concentrations of natural motilin and motilin derivative compounds were measured by the RIA method.
- a calibration curve was prepared using each derivative as a standard substance, and the plasma concentration was determined.
- FIG. 3 shows changes in plasma concentrations of these compounds after pulmonary administration of natural motilin, compound 2 (MT114) and compound 6 (MT140).
- Compound 2 (MT114) and Compound 6 (MT140) were pulmonary administered to rats compared with the transition of plasma motilin concentration after pulmonary administration of natural motilin to rats. Later plasma compound concentrations were higher in peak plasma concentrations after administration, indicating that higher concentrations could be maintained in vivo for longer periods of time compared to natural motilin.
- Cmax The maximum plasma concentration (Cmax) and the area under the plasma concentration curve (AUC) were calculated as pharmacokinetic parameters from the time course of plasma natural motilin or motilin derivative compound concentration obtained by these tests.
- Cmax was determined from the actual measurement value and AUC was determined by the trapezoidal method.
- bioavailability bioavailability: BA (%) was calculated by the following formula.
- BA (%) [(AUC / Dose) / (AUC (motilin_iv) / Dose (motilin_iv))] ⁇ 100
- AUC AUC after pulmonary administration (ng ⁇ min / mL)
- Dose Dose for transpulmonary administration ( ⁇ g / kg)
- Comparative Example 2 Pharmacokinetic Experiment of Natural Motilin and Motilin Derivative Compound by Intravenous Administration Using Monkeys
- natural motilin and motilin derivative compounds were intravenously administered to monkeys, and plasma concentrations were measured.
- the collected blood was immediately added with 1/100 volume of 10% EDTA ⁇ 2Na ⁇ 2H 2 O solution and centrifuged to separate the plasma. Immediately 1/10 volume of 5,000 IU / mL aprotinin solution was added to the plasma, mixed, and stored at ⁇ 80 ° C. until measurement.
- the plasma concentrations of natural motilin and motilin derivative compounds were measured by a radioimmunoassay (RIA) method using an anti-motilin antibody. That is, after adding an anti-motilin antibody to a plasma sample, [ 125 I-Tyr7] motilin was added to cause a competitive reaction. A secondary antibody was added thereto to precipitate the motilin bound to the anti-motilin antibody, and after separation of the supernatant, the radioactivity in the precipitated fraction was measured with a ⁇ -counter (Perkin Elmer).
- RIA radioimmunoassay
- the resulting plasma natural motilin concentration transition is shown in FIG. Further, from the plasma concentration transition of the obtained natural type motilin and motilin derivative compound, the plasma concentration at time 0 (C0) and the area under the plasma concentration curve (AUC) were calculated as pharmacokinetic parameters. C0 was obtained by extrapolation, and AUC was obtained by trapezoidal method. As a result, when natural motilin and Compound 2 were intravenously administered at a dose of 10 ⁇ g / kg, C0 was 233 and 273 ng / mL, and AUC was calculated as 786 and 926 ng ⁇ min / mL.
- Example 5 Pharmacokinetic experiment of natural type motilin and motilin derivative compounds by nasal administration using monkeys
- natural type motilin and motilin derivative compounds were administered nasally to monkeys, and then the plasma concentration of The purpose was to measure changes by RIA method.
- a nasal preparation was prepared by mixing natural motilin or compound 2 and crystalline cellulose in a ratio of 1:40, and 20 mg was filled in a gelatin capsule.
- One capsule was administered into the right nasal cavity of a cynomolgus monkey using a nasal administration device (manufactured by Hitachi Automotive System). Blood was collected from the cephalic vein before administration and 5, 10, 15, 20, 30, 60, 120 and 180 minutes after administration. The collected blood was treated in the same manner as in Comparative Example 2 to separate plasma, and the plasma concentrations of natural motilin and motilin derivative compounds were measured by the RIA method. In the measurement, a calibration curve was prepared using each derivative as a standard substance, and the plasma concentration was determined.
- FIG. 5 shows changes in plasma concentrations of these compounds after nasal administration of natural motilin and compound 2 (MT114) to monkeys.
- the plasma compound concentration after nasally administering Compound 2 (MT114) to monkeys was compared with the transition of plasma motilin concentration after nasally administering natural motilin to monkeys.
- the transition of plasma motilin concentration after nasally administering natural motilin to monkeys was found to have a high peak plasma concentration after administration, and to maintain a high concentration in vivo for a longer period of time compared to natural motilin.
- Cmax The maximum plasma concentration (Cmax) and the area under the plasma concentration curve (AUC) were calculated as pharmacokinetic parameters from the time course of plasma natural motilin or motilin derivative compound concentration obtained by these tests.
- Cmax was determined from the actual measurement value and AUC was determined by the trapezoidal method.
- bioavailability bioavailability: BA (%) was calculated by the following formula.
- BA (%) [(AUC / Dose) / (AUC (iv) / Dose (iv))] x 100
- AUC AUC after nasal administration (ng ⁇ min / mL)
- Dose Dose for nasal administration ( ⁇ g / kg)
- the bioavailability (hereinafter abbreviated as BA) when Compound 2 was administered nasally to monkeys was 5.1%. It was higher than that (3.6%).
- BA bioavailability
- the absorption efficiency from the mucosa to the living body is improved, and as a result, absorption compared to natural type motilin is improved. It has been shown that the concentration of the compound in vivo remains high for a longer time.
- the present invention relates to a novel motilin-like peptide compound. Since the motilin-like peptide compound of the present invention or a pharmaceutically acceptable salt thereof has effective gastrointestinal motility stimulating activity and high absorbability in transmucosal administration, diseases caused by gastrointestinal dysfunction (eg, gastrointestinal tract) Symptoms characterized by reduced basal levels of motor activity can be treated. Diseases caused by gastrointestinal dysfunction include functional dyspepsia, diabetic gastric paresis, gastroesophageal reflux disease, irritable bowel syndrome, small intestinal bacterial hyperproliferation, colonic pseudoobstruction, paralytic intestinal obstruction, chronic idiopathic Symptoms such as sexual pseudo-intestinal obstruction and postoperative intestinal obstruction.
- gastrointestinal dysfunction eg, gastrointestinal tract
- Diseases caused by gastrointestinal dysfunction include functional dyspepsia, diabetic gastric paresis, gastroesophageal reflux disease, irritable bowel syndrome, small intestinal bacterial hyperproliferation, colonic pseudoobstruction, paralytic intestinal obstruction
- SEQ ID NO: 1 is the amino acid sequence of human motilin.
- SEQ ID NO: 2 is the amino acid sequence of the motilin analog.
- SEQ ID NO: 3 is the amino acid sequence of compound 1 (MT095).
- SEQ ID NO: 4 is the amino acid sequence of Compound 2 (MT114).
- SEQ ID NO: 5 is the amino acid sequence of compound 3 (MT116).
- SEQ ID NO: 6 is the amino acid sequence of compound 4 (MT124).
- SEQ ID NO: 7 is the amino acid sequence of compound 5 (MT126).
- SEQ ID NO: 8 is the amino acid sequence of compound 6 (MT140).
- SEQ ID NO: 9 is the amino acid sequence of compound 7 (MT141).
- SEQ ID NO: 10 is the amino acid sequence of Compound 8 (MT107).
- SEQ ID NO: 11 is the amino acid sequence of compound 9 (MT115).
- [SEQ ID NO: 12] is the amino acid sequence of compound 10 (MT125).
- SEQ ID NO: 13 is the amino acid sequence of Compound 11 (MT128).
- SEQ ID NO: 14 is the amino acid sequence of Compound 12 (MT154).
- SEQ ID NO: 15 is the amino acid sequence of compound 13 (MT155).
- SEQ ID NO: 16 is the amino acid sequence of comparative compound 1 ( 13 Leu-motilin).
- SEQ ID NO: 17 is the amino acid sequence of Comparative Compound 2 (MT139).
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Nutrition Science (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
X1 Val X2 Ile Phe Thr Tyr Gly X3 Leu Gln Arg X4 Gln Glu Lys Glu Arg X5 Lys Pro Gln (式1)(SEQ ID NO: 2)
〔式中、各アミノ酸間の結合は、X1-Val間の結合以外は全てアミド結合であり;
X1は芳香族アミノ酸または複素芳香族アミノ酸であり;
X1-Val間の結合はアミド結合または以下の式2
X2はプロリンまたはサルコシンであり;
X3はグルタミン酸またはアスパラギン酸であり;
X4はメチオニンまたはロイシンであり;
X5はアスパラギンまたはプロリンである〕
により示される配列を含む化合物、またはその薬学的に許容可能な塩であり、天然型モチリンと同等の消化管運動刺激活性と経粘膜投与における高い吸収性をあわせ持つペプチド化合物を作製することにより、上述した課題を解決することが出来ることを明らかにした。
(ii)(式1)のX1がフェニルアラニン(Phe)、チロシン(Tyr)、およびトリプトファン(Trp)等のα-アミノ酸の他、β-ホモフェニルグリシン(Phg(C#CH2))、アセチルナフチルアラニン(Ac-Nal)である、(i)に記載の化合物またはその薬学的に許容可能な塩。
(iii)(式1)のX1がフェニルアラニンまたはβ-ホモフェニルグリシンである、(ii)に記載の化合物またはその薬学的に許容可能な塩。
(iv)SEQ ID NO: 3~15から成る群から選択される配列で示される化合物またはその薬学的に許容可能な塩。
(v)SEQ ID NO: 4で示される化合物またはその薬学的に許容可能な塩。
(vi)SEQ ID NO: 6で示される化合物またはその薬学的に許容可能な塩。
(vii)SEQ ID NO: 8で示される化合物またはその薬学的に許容可能な塩。
(viii) (i)~(vii)のいずれか1項に記載のペプチド化合物またはその薬学的に許容される塩を含む、消化管機能異常を伴う疾患の治療用医薬組成物。
(ix)消化管機能異常を伴う疾患が消化管運動活性の基礎レベルの低下を伴うものである(viii)に記載の医薬組成物。
(x)消化管機能異常を伴う疾患が機能性ディスペプシア、糖尿病性胃不全麻痺、胃食道逆流症、過敏性腸症候群、小腸内細菌過剰増殖症、結腸偽性閉塞症、麻痺性腸閉塞、慢性特発性偽性腸閉塞または術後性腸閉塞である(viii)に記載の医薬組成物。
(xi)医薬組成物が経粘膜投与用である(viii)~(x)のいずれか1項に記載の医薬組成物。
(xii)経粘膜投与が経肺投与または経鼻投与である(xi)に記載の医薬組成物。
(xiii)経粘膜投与が経鼻投与である(xii)に記載の医薬組成物。
(xiv) (viii)に記載の医薬組成物を個体に投与することを含む、機能性ディスペプシア、糖尿病性胃不全麻痺、胃食道逆流症、過敏性腸症候群、小腸内細菌過剰増殖症、結腸偽性閉塞症、麻痺性腸閉塞、慢性特発性偽性腸閉塞、および術後性腸閉塞等の消化管運動活性の基礎レベルの低下を特徴とする症状の治療方法。
X1 Val X2 Ile Phe Thr Tyr Gly X3 Leu Gln Arg X4 Gln Glu Lys Glu Arg X5 Lys Pro Gln (式1)(SEQ ID NO: 2)
〔式中、各アミノ酸間の結合はX1-Val間の結合以外は全てアミド結合であり;
X1-Val間の結合はアミド結合または以下の式2
X1は芳香族アミノ酸または複素芳香族アミノ酸であり;
X2はプロリンまたはサルコシンであり;
X3はグルタミン酸またはアスパラギン酸であり;
X4はメチオニンまたはロイシンであり;
X5はアスパラギンまたはプロリンである〕
により示される配列を含む化合物、またはその薬学的に許容可能な塩であり、天然型モチリンと同様の消化管運動刺激活性と経粘膜投与における高い吸収性をあわせ持つ特徴を有する。
Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 3);
Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 4);
Phe Val Pro Ile Phe Thr Tyr Gly Asp Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 5);
Phg(C#CH2) Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 6);
Phg(C#CH2) Val Sar Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 7);
Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 8);
Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 9);
PheValProIlePheThrTyrGlyGluLeuGlnArgMetGlnGluLysGluArgProLysProGln(SEQ ID NO: 10);
PheValProIlePheThrTyrGlyGluLeuGlnArgLeuGlnGluLysGluArgProLysProGln(SEQ ID NO: 11);
Phg(C#CH2)ValSarIlePheThrTyrGlyGluLeuGlnArgMetGlnGluLysGluArgProLysProGln(SEQ ID NO: 12);
Ac-NalValProIlePheThrTyrGlyGluLeuGlnArgMetGlnGluLysGluArgAsnLysProGln(SEQ ID NO: 13);
Phe ValProIlePheThrTyrGlyAspLeuGlnArgLeuGlnGluLysGluArgProLysProGln(SEQ ID NO: 14);
Phe ValProIlePheThrTyrGlyAspLeuGlnArgMetGlnGluLysGluArgProLysProGln(SEQ ID NO: 15)。
Phg(C#CH2):L-β-ホモフェニルグリシン
Ac-Nal:アセチルナフチルアラニン
Sar:L-サルコシン
CH2-NH:-psi[CH2NH]-結合(シュード結合ともいう)
Fmoc:フルオレニルメチルオキシカルボニル
Boc:t-ブチルオキシカルボニル
TFA:トリフルオロ酢酸
Trt:トリチル
HBTU:N-[(1H-ベンゾトリアゾール-1-イル(ジメチルアミノ)メチレン]-N-メチルメタンアミニウムヘキサフルオロホスフェートN-オキシド
HOBt:1-ヒドロキシベンゾトリアゾール
DCC:N,N'-ジシクロヘキシルカルボジイミド
TIPS:トリイソプロピルシラン。
モチリンの分解酵素による分解経路、13位メチオニンの安定性、またモチリン生理活性の維持などを考え、以下に示すモチリン類似ペプチド化合物を合成した。また比較対象として、天然型モチリンの合成も実施した。比較化合物1および2、化合物1~13のアミノ酸配列は以下の通りである。
比較化合物1(13Leu-モチリン):Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Gly Gln(SEQ ID NO: 16);
比較化合物2(MT139):Phe*Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Gly Gln(SEQ ID NO: 17);
化合物1(MT095):Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 3);
化合物2(MT114):Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 4);
化合物3(MT116):Phe Val Pro Ile Phe Thr Tyr Gly Asp Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 5);
化合物4(MT124):Phg(C#CH2) Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 6);
化合物5(MT126):Phg(C#CH2) Val Sar Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 7);
化合物6(MT140):Phe*Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 8);
化合物7(MT141):Phe*Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 9);
化合物8(MT107):Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 10);
化合物9(MT115):Phe Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 11);
化合物10(MT125):Phg(C#CH2)ValSarIlePheThrTyrGlyGluLeuGlnArgMetGlnGluLysGluArgProLysProGln(SEQ ID NO: 12);
化合物11(MT128):Ac-Nal Val Pro Ile Phe Thr Tyr Gly Glu Leu Gln Arg Met Gln Glu Lys Glu Arg Asn Lys Pro Gln(SEQ ID NO: 13);
化合物12(MT154):Phe*Val Pro Ile Phe Thr Tyr Gly Asp Leu Gln Arg Leu Gln Glu Lys Glu Arg Pro Lys Pro Gln(SEQ ID NO: 14);
化合物13(MT155):Phe*ValProIlePheThrTyrGlyAspLeuGlnArgMetGlnGluLysGluArgProLysProGln(SEQ ID NO: 15)。
本実施例は、化合物1(MT095、SEQ ID NO: 3)を化学的に合成することを目的として行った。
本実施例は、化合物6(MT140、SEQ ID NO: 8)を化学的に合成することを目的として行った。
本実施例は、実施例1および2に記載された方法により作製された化合物を用いて、イヌの体内における空腹期の肛側伝播性強収縮帯(IMC)惹起活性を測定することを目的として行った。
天然型モチリンをラットに静脈内投与し、血漿中濃度を測定した。
本実施例においては、天然型モチリンおよびモチリン誘導体化合物をラットに経肺投与し、その後の血漿中濃度の変化をRIA法を用いて測定することを目的とした。なおRIA法を用いた場合、抗モチリン抗体と抗原抗体反応を示す物質が全て検出されるため、未変化体のほかに抗体に反応する代謝物をも検出する可能性がある。そこで天然型モチリンおよびモチリン誘導体化合物をラットに経肺投与し、得られた血漿をHPLC分画して各画分中の免疫活性を測定することにより、抗体に反応する物質が未変化体であることを明らかにし、血漿中濃度の変化を測定方法としてRIA法が適していることを確認した。
BA(%)=[(AUC/Dose)/(AUC(motilin_iv)/Dose(motilin_iv))]×100
AUC:経肺投与後のAUC(ng・分/mL)
Dose:経肺投与における投与量(μg/kg)
AUC(motilin_iv):天然型モチリン静脈内投与後のAUC(ng・分/mL)
Dose(motilin_iv):天然型モチリン静脈内投与における投与量(μg/kg)。
本比較例では、天然型モチリンおよびモチリン誘導体化合物をサルに静脈内投与し、血漿中濃度を測定した。
本実施例においては、天然型モチリンおよびモチリン誘導体化合物をサルに経鼻投与し、その後の血漿中濃度の変化をRIA法により測定することを目的とした。
BA(%)=〔(AUC/Dose)/(AUC(iv)/Dose(iv))〕×100
AUC:経鼻投与後のAUC(ng・分/mL)
Dose:経鼻投与における投与量(μg/kg)
AUC(iv):各化合物を静脈内投与後のAUC(ng・分/mL)
Dose(iv):各化合物を静脈内投与における投与量(μg/kg)。
Claims (7)
- 次の一般式1:
X1 Val X2 Ile Phe Thr Tyr Gly X3 Leu Gln Arg X4 Gln Glu Lys Glu Arg X5 Lys Pro Gln(式1)
〔式中、各アミノ酸間の結合はX1-Val間の結合以外は全てアミド結合であり;
X1-Val間の結合はアミド結合または以下の式2
X1は芳香族アミノ酸または複素芳香族アミノ酸であり;
X2はプロリンまたはサルコシンであり;
X3はグルタミン酸またはアスパラギン酸であり;
X4はメチオニンまたはロイシンであり;
X5はアスパラギンまたはプロリンである〕
で表される配列で示される化合物、またはその薬学的に許容可能な塩。 - X1がフェニルアラニンまたはβ-ホモフェニルグリシンである、請求項1に記載の化合物、またはその薬学的に許容可能な塩。
- SEQ ID NO:3ないし15から成る群から選択される配列で示される化合物、またはその薬学的に許容可能な塩。
- 請求項1ないし3のいずれかひとつに記載される化合物、またはその薬学的に許容可能な塩を含む、消化管機能異常を伴う疾患の治療用医薬組成物。
- 消化管機能異常を伴う疾患が、機能性ディスペプシア、糖尿病性胃不全麻痺、胃食道逆流症、過敏性腸症候群、小腸内細菌過剰増殖症、結腸偽性閉塞症、麻痺性腸閉塞、慢性特発性偽性腸閉塞、および術後性腸閉塞からなる群から選択される、請求項4に記載の医薬組成物。
- 医薬組成物が経粘膜投与用医薬組成物である、請求項4または5に記載の医薬組成物。
- 経粘膜投与が経肺または経鼻である、請求項6に記載の医薬組成物。
Priority Applications (16)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10804474.4A EP2460826B1 (en) | 2009-07-29 | 2010-07-29 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
BR112012000719A BR112012000719A2 (pt) | 2009-07-29 | 2010-07-29 | composto de peptídeo do tipo motilina tendo absorção transmucosal concendida ao mesmo |
RU2012107290/10A RU2540012C2 (ru) | 2009-07-29 | 2010-07-29 | Мотилин-подобное пептидное соединение, обладающее приданной ему трансмукозальной абсорбционной способностью |
JP2011524821A JP5647609B2 (ja) | 2009-07-29 | 2010-07-29 | 経粘膜吸収性を付与したモチリン類似ペプチド化合物 |
SG2012001715A SG177592A1 (en) | 2009-07-29 | 2010-07-29 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
US13/388,021 US8710184B2 (en) | 2009-07-29 | 2010-07-29 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
CN201080043683.9A CN102574904B (zh) | 2009-07-29 | 2010-07-29 | 赋予了经粘膜吸收性的胃动素样肽化合物 |
AU2010279076A AU2010279076B2 (en) | 2009-07-29 | 2010-07-29 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
MX2012001193A MX2012001193A (es) | 2009-07-29 | 2010-07-29 | Compuesto peptidico similar a motilina con capacidad de absorcion transmucosa impartida al mismo. |
IN569DEN2012 IN2012DN00569A (ja) | 2009-07-29 | 2010-07-29 | |
ES10804474.4T ES2653523T3 (es) | 2009-07-29 | 2010-07-29 | Compuesto peptídico de tipo motilina que tiene una susceptibilidad de absorción transmucosa impartida al mismo |
NZ597777A NZ597777A (en) | 2009-07-29 | 2010-07-29 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
CA2769013A CA2769013C (en) | 2009-07-29 | 2010-07-29 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
KR1020127002980A KR101789605B1 (ko) | 2009-07-29 | 2010-07-29 | 경점막 흡수성을 가진 모틸린 유사 펩타이드 화합물 |
ZA2012/00440A ZA201200440B (en) | 2009-07-29 | 2012-01-19 | Motilin-like peptide compound having transmucosal absorbability imparted thereto |
IL217781A IL217781A (en) | 2009-07-29 | 2012-01-26 | A peptide compound similar to mucilage with mucosal uptake |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009177107 | 2009-07-29 | ||
JP2009-177107 | 2009-07-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2011013728A1 true WO2011013728A1 (ja) | 2011-02-03 |
Family
ID=43529381
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2010/062746 WO2011013728A1 (ja) | 2009-07-29 | 2010-07-29 | 経粘膜吸収性を付与したモチリン類似ペプチド化合物 |
Country Status (20)
Country | Link |
---|---|
US (1) | US8710184B2 (ja) |
EP (1) | EP2460826B1 (ja) |
JP (1) | JP5647609B2 (ja) |
KR (1) | KR101789605B1 (ja) |
CN (1) | CN102574904B (ja) |
AU (1) | AU2010279076B2 (ja) |
BR (1) | BR112012000719A2 (ja) |
CA (1) | CA2769013C (ja) |
CO (1) | CO6430471A2 (ja) |
ES (1) | ES2653523T3 (ja) |
IL (1) | IL217781A (ja) |
IN (1) | IN2012DN00569A (ja) |
MX (1) | MX2012001193A (ja) |
MY (1) | MY161842A (ja) |
NZ (1) | NZ597777A (ja) |
RU (1) | RU2540012C2 (ja) |
SG (1) | SG177592A1 (ja) |
TW (1) | TWI471138B (ja) |
WO (1) | WO2011013728A1 (ja) |
ZA (1) | ZA201200440B (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016532684A (ja) * | 2013-07-31 | 2016-10-20 | ライナット ニューロサイエンス コーポレイション | 操作されたポリペプチドコンジュゲート |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2792749C (en) * | 2010-03-19 | 2016-08-23 | Daiichi Sankyo Company, Limited | Composition for nasal administration and method for preparing it |
KR101470793B1 (ko) | 2014-06-30 | 2014-12-08 | 순천향대학교 산학협력단 | 흡수촉진제로서의 펩타이드와 이를 포함하는 조성물 |
RU2662310C9 (ru) * | 2015-12-04 | 2018-09-21 | Виктор Вениаминович Тец | Средство (варианты) и способы восстановления микрофлоры |
CN107383169A (zh) * | 2017-04-24 | 2017-11-24 | 青岛大学 | 一种具有胃肠运动刺激活性的多肽 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5246068A (en) | 1975-10-03 | 1977-04-12 | Max Planck Gesellschaft | Production of llleucinee 133mochilin and compound containing the same |
JPH03218395A (ja) * | 1989-01-06 | 1991-09-25 | Sanwa Kagaku Kenkyusho Co Ltd | モチリン様活性を有するポリペプチド及びその用途 |
JPH0770178A (ja) | 1993-08-06 | 1995-03-14 | Ohmeda Pharmaceut Prod Division Inc | 胃腸運動刺激活性を有するモチリン類似ポリペプチド |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2753274B2 (ja) * | 1988-08-24 | 1998-05-18 | 株式会社三和化学研究所 | モチリン様ポリペプチドの製法並びにそのための組換えdna及び発現用プラスミド |
EP0378078A1 (en) * | 1989-01-06 | 1990-07-18 | Sanwa Kagaku Kenkyusho Co., Ltd. | Motilin-like polypeptide and use thereof |
DE3922764A1 (de) | 1989-07-11 | 1991-01-17 | Babcock Werke Ag | Verfahren und vorrichtung zum abtrennen von feststoff aus einem gas |
JPH04364131A (ja) * | 1991-04-03 | 1992-12-16 | Sanwa Kagaku Kenkyusho Co Ltd | 易吸収性モチリン製剤 |
BR9808059A (pt) * | 1997-03-24 | 2000-03-08 | Zimogenetics Inc | Molécula de plinucleotìdeo isolada codificando um polipeptìdeo, polinucleotìdeo, vetor de expressão, célula cultivada, polipeptìdeo isolado, composição farmacêutica, anticorpo, processos para produzir polipeptìdeo zsig33, e, para estimular a motilidde gástrica |
US5972939A (en) * | 1997-10-28 | 1999-10-26 | Ortho-Mcneil Pharmaceutical, Inc. | Cyclopentene derivatives useful as antagonists of the motilin receptor |
SE9802080D0 (sv) * | 1998-06-11 | 1998-06-11 | Hellstroem | Pharmaceutical composition for the treatment of functional dyspepsia and/or irritable bowel syndrome and new use of substances therein |
US6849714B1 (en) * | 1999-05-17 | 2005-02-01 | Conjuchem, Inc. | Protection of endogenous therapeutic peptides from peptidase activity through conjugation to blood components |
US6887470B1 (en) * | 1999-09-10 | 2005-05-03 | Conjuchem, Inc. | Protection of endogenous therapeutic peptides from peptidase activity through conjugation to blood components |
DK1105409T3 (da) * | 1999-05-17 | 2006-07-03 | Conjuchem Inc | Beskyttelse af endogene terapeutiske peptider fra peptidaseaktivitet ved konjugering med blodkomponenter |
US6511980B2 (en) * | 2000-05-05 | 2003-01-28 | Ortho Mcneil Pharmaceutical, Inc. | Substituted diamine derivatives useful as motilin antagonists |
RU2252779C1 (ru) * | 2003-09-23 | 2005-05-27 | Институт Молекулярной Генетики Российской Академии Наук (Имг Ран) | Способ профилактики и лечения язв желудочно-кишечного тракта |
CA2792749C (en) * | 2010-03-19 | 2016-08-23 | Daiichi Sankyo Company, Limited | Composition for nasal administration and method for preparing it |
-
2010
- 2010-07-29 IN IN569DEN2012 patent/IN2012DN00569A/en unknown
- 2010-07-29 US US13/388,021 patent/US8710184B2/en not_active Expired - Fee Related
- 2010-07-29 MY MYPI2012000392A patent/MY161842A/en unknown
- 2010-07-29 NZ NZ597777A patent/NZ597777A/xx not_active IP Right Cessation
- 2010-07-29 MX MX2012001193A patent/MX2012001193A/es active IP Right Grant
- 2010-07-29 JP JP2011524821A patent/JP5647609B2/ja not_active Expired - Fee Related
- 2010-07-29 EP EP10804474.4A patent/EP2460826B1/en not_active Not-in-force
- 2010-07-29 ES ES10804474.4T patent/ES2653523T3/es active Active
- 2010-07-29 WO PCT/JP2010/062746 patent/WO2011013728A1/ja active Application Filing
- 2010-07-29 CN CN201080043683.9A patent/CN102574904B/zh not_active Expired - Fee Related
- 2010-07-29 SG SG2012001715A patent/SG177592A1/en unknown
- 2010-07-29 AU AU2010279076A patent/AU2010279076B2/en not_active Ceased
- 2010-07-29 RU RU2012107290/10A patent/RU2540012C2/ru not_active IP Right Cessation
- 2010-07-29 BR BR112012000719A patent/BR112012000719A2/pt not_active Application Discontinuation
- 2010-07-29 KR KR1020127002980A patent/KR101789605B1/ko active IP Right Grant
- 2010-07-29 TW TW99125019A patent/TWI471138B/zh not_active IP Right Cessation
- 2010-07-29 CA CA2769013A patent/CA2769013C/en not_active Expired - Fee Related
-
2012
- 2012-01-19 ZA ZA2012/00440A patent/ZA201200440B/en unknown
- 2012-01-26 IL IL217781A patent/IL217781A/en not_active IP Right Cessation
- 2012-02-13 CO CO12024411A patent/CO6430471A2/es active IP Right Grant
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5246068A (en) | 1975-10-03 | 1977-04-12 | Max Planck Gesellschaft | Production of llleucinee 133mochilin and compound containing the same |
JPH03218395A (ja) * | 1989-01-06 | 1991-09-25 | Sanwa Kagaku Kenkyusho Co Ltd | モチリン様活性を有するポリペプチド及びその用途 |
JPH0742319B2 (ja) | 1989-01-06 | 1995-05-10 | 株式会社三和化学研究所 | モチリン様活性を有するポリペプチド及びその用途 |
JPH0770178A (ja) | 1993-08-06 | 1995-03-14 | Ohmeda Pharmaceut Prod Division Inc | 胃腸運動刺激活性を有するモチリン類似ポリペプチド |
Non-Patent Citations (33)
Title |
---|
"Seikagaku Jikken Koza 1 Tanpakushitu no Kagaku", vol. 4, TOKYO KAGAKU DOJIN |
"Zoku Iyakuhinn no Kaihatsu 14 Peptide Gosei", HIROKAWA SHOTEN |
ANDERSSON A.; MALER L., J. BIOMOL. NMR, vol. 24, 2002, pages 103 - 112 |
BOULANGER YVAN. ET AL.: "Structural effects of the selective reduction of amide carbonyl groups in motilin 1-12 as determined by nuclear magnetic resonance.", INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, vol. 46, 1995, pages 527 - 534, XP008149329 * |
BROWN J ET AL., CAN J BIOCHEM, vol. 51, 1973, pages 533 |
CASTIGLIONE F ET AL., ALIMENT PHARMACOL THER., vol. 18, 2003, pages 1107 |
CHOI MG ET AL., J PHARMACOL EXP THER, vol. 285, 1998, pages 37 |
DE CLERCQ ET AL., REGUL PEPT, vol. 55, 1995, pages 79 |
GASTROENTEROLOGY, vol. 130, 2006, pages 1377 - 1556 |
HAANS J ET AL., NEUROGASTROENTEROL MOTIL, vol. 18, 2006, pages 637 |
HENRY C. LIN, JAMA, vol. 292, 2004, pages 852 |
ITOH; NISSHOUSHI, JOURNAL OF THE JAPANESE SOCIETY OF GASTROGENTEROLOGY, vol. 93, 1996, pages 517 |
JANSSENS J ET AL., N ENGL J MED, vol. 322, 1990, pages 1028 |
JENSSEN TG ET AL., SCAND J GASTROENTEROL, vol. 19, 1984, pages 717 |
KAMERLING I ET AL., AM J PHYSIOL GASTROINTEST LIVER PHYSIOL, vol. 284, 2003, pages G776 |
KUSANO ET AL., MB GASTRO, vol. 1, 1991, pages 47 |
KUSANO M ET AL., AM J GASTROENTEROL, vol. 92, 1997, pages 481 |
LABO G ET AL., GASTROENTEROLOGY, vol. 90, 1986, pages 20 |
MILLER P ET AL., PEPTIDES, vol. 16, 1995, pages 11 - 18 |
MILLER P. ET AL., PEPTIDES, vol. 16, 1995, pages 11 - 18 |
NAKAYA M ET AL., PEPTIDES, vol. 4, 1983, pages 439 |
NETZER P ET AL., ALIMENT PHARMACOL THER, vol. 16, 2002, pages 1481 |
PARDO A ET AL., HEPATOLOGY, vol. 31, 2000, pages 858 |
PARK M-I ET AL., NEUROGASTROENTEROL MOTIL, vol. 18, 2006, pages 28 |
PAUL MILLER. ET AL.: "Structure-function studies of motilin analogues", PEPTIDES, vol. 16, 1995, pages 11 - 18, XP002040813 * |
PEETERS T.L. ET AL., PEPTIDES, vol. 13, 1992, pages 1103 - 1107 |
PEETERS TL ET AL., NEUROGASTROENTEROL MOTIL, vol. 18, 2006, pages 1 |
PIMENTEL M ET AL., DIG DIS SCI, vol. 47, 2002, pages 2639 |
SCHUBERT H ET AL., CAN J BIOCHEM, vol. 52, 1974, pages 7 |
See also references of EP2460826A4 |
STACHER G ET AL., GUT, vol. 34, 1993, pages 166 |
YOGO K ET AL., DIG DIS SCI, vol. 52, 2007, pages 3112 |
ZEN ITOH ET AL., GASTROENTEROLOGIA JAPONICA, vol. 12, 1977, pages 275 - 283 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016532684A (ja) * | 2013-07-31 | 2016-10-20 | ライナット ニューロサイエンス コーポレイション | 操作されたポリペプチドコンジュゲート |
Also Published As
Publication number | Publication date |
---|---|
RU2540012C2 (ru) | 2015-01-27 |
CA2769013C (en) | 2015-04-07 |
MX2012001193A (es) | 2012-03-14 |
TW201106964A (en) | 2011-03-01 |
IL217781A (en) | 2015-09-24 |
BR112012000719A2 (pt) | 2016-11-01 |
CN102574904B (zh) | 2014-11-26 |
AU2010279076B2 (en) | 2014-09-25 |
CO6430471A2 (es) | 2012-04-30 |
IL217781A0 (en) | 2012-03-29 |
EP2460826B1 (en) | 2017-10-18 |
IN2012DN00569A (ja) | 2015-06-12 |
CA2769013A1 (en) | 2011-02-03 |
US8710184B2 (en) | 2014-04-29 |
SG177592A1 (en) | 2012-02-28 |
JPWO2011013728A1 (ja) | 2013-01-10 |
ES2653523T3 (es) | 2018-02-07 |
KR101789605B1 (ko) | 2017-10-25 |
AU2010279076A1 (en) | 2012-03-01 |
JP5647609B2 (ja) | 2015-01-07 |
CN102574904A (zh) | 2012-07-11 |
RU2012107290A (ru) | 2013-09-10 |
EP2460826A1 (en) | 2012-06-06 |
ZA201200440B (en) | 2013-04-24 |
KR20120052274A (ko) | 2012-05-23 |
NZ597777A (en) | 2013-03-28 |
TWI471138B (zh) | 2015-02-01 |
EP2460826A4 (en) | 2013-11-20 |
MY161842A (en) | 2017-05-15 |
US20120129762A1 (en) | 2012-05-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11008375B2 (en) | GIP-GLP-1 dual agonist compounds and methods | |
US11814417B2 (en) | GIP agonist compounds and methods | |
AU2014345569A1 (en) | GIP-GLP-1 dual agonist compounds and methods | |
NZ591178A (en) | Glucagon-like-peptide-2 (glp-2) analogues | |
JP5647609B2 (ja) | 経粘膜吸収性を付与したモチリン類似ペプチド化合物 | |
KR102520348B1 (ko) | 페길화 생활성 펩타이드 및 그의 용도 | |
AU2018318672A1 (en) | Acylated oxyntomodulin peptide analog | |
CN114787183A (zh) | 肠促胰岛素类似物及其用途 | |
US6949513B2 (en) | Polypeptides of covalently linked synthetic bioactive peptide analog(s) for treatment of cancer |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 201080043683.9 Country of ref document: CN |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 10804474 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2011524821 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 569/DELNP/2012 Country of ref document: IN |
|
REEP | Request for entry into the european phase |
Ref document number: 2010804474 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2010804474 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2769013 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 217781 Country of ref document: IL Ref document number: 12012500169 Country of ref document: PH |
|
WWE | Wipo information: entry into national phase |
Ref document number: MX/A/2012/001193 Country of ref document: MX |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1201000206 Country of ref document: TH |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 13388021 Country of ref document: US |
|
ENP | Entry into the national phase |
Ref document number: 20127002980 Country of ref document: KR Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2010279076 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12024411 Country of ref document: CO |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112012000719 Country of ref document: BR Kind code of ref document: A2 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2012107290 Country of ref document: RU |
|
ENP | Entry into the national phase |
Ref document number: 2010279076 Country of ref document: AU Date of ref document: 20100729 Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 112012000719 Country of ref document: BR Kind code of ref document: A2 Effective date: 20120111 |